[Histonet] RE: Nuclear Artifact

[Podawiltz, Thomas]

I had this issue a couple of years back. Found out that there was a delay of 
over an hour from the time the specimen was collected to the time it was being 
put into fixative. 

Tom 


Tom Podawiltz HT (ASCP)
AP  Section Head 
LRGHealthcare
 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roy, Lisa
Sent: Tuesday, April 21, 2015 1:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nuclear Artifact

Hi HistoNetters:
I have run into quite a problem.  My lab currently processes all tissue types 
from 3 different sites.  Recently, we have been getting complaints from one of 
the sites that the biopsies have a nuclear artifact.  It is described as 
washed out or poor to no nuclear detail.  Pictures have been uploaded 
(Nuclear Artifact).  The Medical Director at said site is convinced that a 
processor error is occurring.  Our site is not seeing this on any of our 
slides.  Biopsies from all three sites are processed and embed together.  We 
have done all trouble shooting that we can think of.  Leica service has come to 
inspect our Peloris processor and all areas checked out as functioning 
properly.  The problem is not consistent daily.  Seems to be worst toward the 
end of the week.
We have been running the same processing protocol, staining protocol and 
cutting protocols for years now.  This problem has just developed over the last 
2 months.  Any ideas, no matter how far-fetched, would be greatly appreciated 
at this point.

Lisa Roy, HT(ASCP)
Histology Supervisor
LabCorp at St. Vincent Hospital
123 Summer St
Worcester, MA
(508)363-9420

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RE: [Histonet] prevent wrinkles when cutting

[Podawiltz, Thomas]

Check you blade angle. Keep your blocks cold, change your blade often and just 
be patient. 


Tom Podawiltz HT (ASCP)
AP  Section Head 
LRGHealthcare
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rachel M 
Gonzalez
Sent: Monday, April 20, 2015 12:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] prevent wrinkles when cutting

Hi

Thursday was the first time I ever used a microtome I move to a lab that 
does not have someone dedicated to cutting. I already miss her.

I have no problems getting ribbons of 10-30 sections long but the pieces are 
half the size of the original block. I am guessing they are wrinkling.
What am I doing wrong?

Thanks
Rachel
Senior Scientist
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RE: [Histonet] BS in Histotechnology

[Podawiltz, Thomas]

Tim for a Pathology Manager you seem to have a low opinion of the education and 
training of the Histo Techs that work for you. Is your training program 
accredited with one of the Histology schools or is your staff left to rend for 
themselves? 

By the way, the lab that I work at basis the starting salaries on your degree 
first, then specialty so MT, HT/HTL with BS degrees earn the same.  

Tom

-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] 
Sent: Tuesday, March 24, 2015 12:42 PM
To: Podawiltz, Thomas; Jennifer MacDonald; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] BS in Histotechnology

Tom, no, Histo does start lower than med techs, but consider that a med tech 
has specialty training from the time they decide to go that route while most 
histotechs have general biology degrees and nothing but on the job training. 
Even with a certification a Histotech is not at the same level as a med  tech 
simply due to the unstructured nature of their self-education and training.  In 
30+ years I have met only a handful of people who got any sort of degrees in 
Histotechnology, so waiting for those people to come along is not going to  
work for hiring. Most of our staff got their certification while working here 
and did it on their own. Only one has a degree in Histotechnology, and a BS at 
that!.

 A starting salary here is $36/hr and it is a $3 to $4 increase per level. The 
lab staff is unionized, and we compete with many large service labs (ie Kaiser) 
and many, many large biotech companies for the same pool of techs. Plus, it is 
expensive to live in the San Francisco Bay Area.

We only recently (a few years ago) started this requirement in order to get our 
staff to a higher level. We still have staff without BA/BS degrees. The degree 
just needs to meet the requirements for certification so does not need to be a 
specialty degree.

Tim

-Original Message-
From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org] 
Sent: Tuesday, March 24, 2015 9:06 AM
To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] BS in Histotechnology

So just out of curiosity is the pay on the same level as that of a Med Tech 
with a BS? 
Does the BA/BS have to be in Histotechnology or is the BA/BS followed by one of 
the on-line certificate programs?  

Tom 


Tom Podawiltz HT (ASCP)
AP  Section Head 
LRGHealthcare
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Tuesday, March 24, 2015 11:47 AM
To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] BS in Histotechnology

Jennifer, we require a BA/BS degree for all Histotechnologist positions. 
However, in our 4 step categories Level 1 does not require certification, just 
the degree and the requirement that they get the certification within a year. 
Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = 
entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 
is Lead tech). Supervisor requires and HTL.

Considering that we already require a BA/BS degree for all levels, the fact a 
person has a HT or HTL is not going to matter much for levels 1 thru 4, only 
for supervisor level.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Monday, March 23, 2015 7:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] BS in Histotechnology

In what areas would a facility hire an HTL over an HT?  Is there a need for 
more HTL programs?  4 Thank you, ___
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RE: [Histonet] BS in Histotechnology

[Podawiltz, Thomas]

So just out of curiosity is the pay on the same level as that of a Med Tech 
with a BS? 
Does the BA/BS have to be in Histotechnology or is the BA/BS followed by one of 
the on-line certificate programs?  

Tom 


Tom Podawiltz HT (ASCP)
AP  Section Head 
LRGHealthcare
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Tuesday, March 24, 2015 11:47 AM
To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] BS in Histotechnology

Jennifer, we require a BA/BS degree for all Histotechnologist positions. 
However, in our 4 step categories Level 1 does not require certification, just 
the degree and the requirement that they get the certification within a year. 
Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = 
entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 
is Lead tech). Supervisor requires and HTL.

Considering that we already require a BA/BS degree for all levels, the fact a 
person has a HT or HTL is not going to matter much for levels 1 thru 4, only 
for supervisor level.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Monday, March 23, 2015 7:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] BS in Histotechnology

In what areas would a facility hire an HTL over an HT?  Is there a need for 
more HTL programs?  4 Thank you, ___
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RE: [Histonet] Embedding Question

[Podawiltz, Thomas]

Not what I call a lean system. Why the change?

Tom 


Tom Podawiltz HT (ASCP)
AP  Section Head 
LRGHealthcare
603-524-3211 ext: 3220




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Thursday, March 12, 2015 9:29 AM
To: HistoNet
Subject: [Histonet] Embedding Question

It has been proposed to move the embedding centers to a room about 210 ft away 
from the tissue processors.

The trip from processor to embedding center would take over 2 minutes and 
require the histotechs to carry the baskets full of cassettes down a much used 
hallway.

Opinions?

Do you feel this is a good idea-yes or no and why?

Thanks in advance,

Paula
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RE: [Histonet] Embedding Question

[Podawiltz, Thomas]

Well said. 

As a tech who once dropped a tray of cassettes on the floor I would be more 
concerned about the travel from the processors to the embedding unit. Just 
imagine a bunch of gastric biopsy cassettes scattered down the hallway and one 
or two open cassettes on the floor.  Increase the travel time and distance and 
increase the risk. 


Tom Podawiltz HT (ASCP)
AP  Section Head 
LRGHealthcare
603-524-3211 ext: 3220




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Thursday, March 12, 2015 1:22 PM
To: Lucie Guernsey
Cc: HistoNet; Morken, Timothy
Subject: Re: [Histonet] Embedding Question

Cooling the paraffin and then re melting will not affect the tissue, unless the 
combined heated, liquified state period becomes extended. Cooling the paraffin 
is a great protector of the tissue, no different than what you have with a 
completed block. Be cautious at how fast and at what temperature you reheat at.
With dry embedding, you have to be cautious about small tissue pieces obtaining 
a drying or heat affect. The small tissue pieces are typically at the bottom of 
the cassette and closest to the heating element, without the insulation of the 
liquid paraffin. Wet embedding there is a possibility of debris tissue 
fragments floating amongst the cassette. Both methods require cleanliness and 
short times in liquid paraffin to embedding. 

Sent from my iPhone

 On Mar 12, 2015, at 10:09 AM, Lucie Guernsey lguern...@ucsd.edu wrote:
 
 If I may, I'd like to piggy-back onto what Paula has mentioned 
 regarding allowing paraffin infiltrated tissue to cool before 
 embedding it. Hopefully someone can help both of us out, even if we 
 seem to warm our infiltrated tissue differently (Paula's in a dry bin and 
 mine in a wax bath).
 
 I work in a research lab where we work in large batches and time is 
 not a priority like it is in a clinical setting. Rather than leaving 
 60-80 cassettes of infiltrated tissue soaking in a hot wax bath for 
 hours at a time, we've begun to allow the cassettes to cool and just 
 toss a handful of cassettes into the wax bath 5-10 minutes before 
 we're ready to embed that batch of cassettes. Sometimes we don't even 
 embed the cooled tissue until the next day or later that week. I 
 haven't noticed an obvious difference in how our blocks section, 
 though we have troublesome batches sometimes and we haven't been able to put 
 our finger on why.
 
 Anyone know if allowing infiltrated tissue to cool and then reheat 
 before embedding is better or worse than keeping the tissue soaking in 
 wax for hours at a time?
 
 Thanks!
 Lucie
 
 Lucie Guernsey
 UC San Diego
 lguern...@ucsd.edu
 
 
 
 On Thu, Mar 12, 2015 at 9:43 AM, Paula Sicurello pat...@gmail.com wrote:
 
 Hi Tim,
 
 There are several embedding events through-out the day, though mostly 
 in the wee hours of the morning.  The embedding centers would be in 
 the same room as the  microtomes (another question about those tomorrow).
 
 I worry about the small (GI, needles, etc) biopsies freezing before 
 they reach the embedding stations.  In my experience, once they 
 freeze they get this outer wax coating (like a permeability barrier) 
 which doesn't melt when placed in the dry (no paraffin inside) but hot, 
 holding bin.
 
 They just don't seem to embed that well and have a tendency to drop 
 out of the sections when cutting.
 
 Has anyone else had that happen?
 
 Paula
 
 On Thu, Mar 12, 2015 at 9:07 AM, Morken, Timothy 
 timothy.mor...@ucsf.edu
 wrote:
 
 Paula,
 How many times per day?
 Is the embedding close to the cutting area?
 
 Of course any extra walking is a problem, especially in busy areas. 
 Is this a non-patient area (hopefully!)? Any restructuring should be 
 to move things closer together, not further away!
 
 Having said that, If it comes to that I would be more concerned 
 about embedding proximity to the cutting area since having embedding 
 near
 cutting
 enhances workflow and cross coverage. If you don't unload processors 
 very often then having them distant might not be too bad. Not ideal, 
 but not a necessarily a deal killer.
 
 
 Tim Morken
 Pathology Site Manager, Parnassus
 Supervisor, Electron Microscopy/Neuromuscular Special Studies 
 Department of Pathology UC San Francisco Medical Center
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula 
 Sicurello
 Sent: Thursday, March 12, 2015 6:29 AM
 To: HistoNet
 Subject: [Histonet] Embedding Question
 
 It has been proposed to move the embedding centers to a room about 
 210 ft away from the tissue processors.
 
 The trip from processor to embedding center would take over 2 
 minutes and require the histotechs to carry the baskets full of 
 cassettes down a much used hallway.
 
 Opinions?
 
 Do you feel this is a good idea-yes or no and why?

[Histonet] RE: Body storage refrigerator temperatures

[Podawiltz, Thomas]

Not at the moment in New England. 

Sorry, I just could not resist with the way this winter has been. 

We did at one time and we ended up replacing the cooling unit in our morgue. 

Tom 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya
Sent: Monday, March 02, 2015 1:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Body storage refrigerator temperatures

Does anyone else have a problem getting their body storage refrigerators to 
maintain the 1.1°C to 4.4°C temps to meet CAP guidelines?
Thanks in advance for your responses!
Tanya


Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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RE: [Histonet] Block Counts

[Podawiltz, Thomas]

I have never really cared how fast or how many blocks can be embedded or cut 
per hour. I have always focused on the quality. In a hospital setting there is 
too much variety to have set numbers. 



Tom Podawiltz HT (ASCP)
Histology Section Head 
LRGHealthcare
Laconia, NH 03246
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Monday, January 12, 2015 6:18 PM
To: Morken, Timothy
Cc: histonet@lists.utsouthwestern.edu; Goins, Tresa; Diana McCaig; Ellen
Subject: Re: [Histonet] Block Counts 

This discussion is exactly why we do not count blocks at microtomy, only 
slides. Counting slides is the equalizer for multiple levels and slides per 
block. Count the most appropriate unit at a task (i. e. slides at microtomy, 
blocks at embedding, specimens at grossing, specimens at accession inc) that 
allows you to set work pace and creates a corresponding quality measure.

William DeSalvo
william.desa...@sonoraquest.com
602-768-3692
Sent from my iPhone

 On Jan 12, 2015, at 4:05 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote:
 
 I agree with Diana, I found we had over a dozen different task mixes in a 
 given day for various techs. That includes mix of block types (bx, extensive 
 lists of stain requests per block, single HE, recuts, mega block, research 
 cases), other tasks (Staining, speicals, ihc, tissue processor . Grossing 
 would be even more complicated. Instead of a per-day count, use per hour or 
 per two hours - Some period when the person is concentrating on a single task 
 without interruption. 
 
 Tim Morken
 Supervisor, Histology, Electron Microscopy and Neuromuscular Special 
 Studies UC San Francisco Medical Center San Francisco, CA
 
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 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana 
 McCaig
 Sent: Monday, January 12, 2015 2:46 PM
 To: 'Goins, Tresa'; Ellen; histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Block Counts
 
 I have always found there are so many variables that having an expectation of 
 setting a set number is not always possible.  I feel they should be compared 
 to their own standards and not of their co-workers.  If they can cut or embed 
 a set number on a particular than they should maintain or gradually increase 
 (for newer techs) over time.  .
 I have seen where in one day they cut so many blocks when the work load 
 mandates it but on a slower day it takes them just as much time to cut half 
 as many blocks.  The work pace should be at their comfort level but should be 
 a standard rate.
 
 Diana
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, 
 Tresa
 Sent: January-12-15 4:58 PM
 To: Ellen; histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Block Counts
 
 Depends on the type of tissue.
 Depends on the length of time your day is for a repetitive task.
 Assigning an arbitrary number is counterproductive.
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen
 Sent: Monday, January 12, 2015 1:57 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Block Counts
 
 I'm looking for raw data on time studies that directly deal with the number 
 of blocks a PA can produce in a day and how many a histo tech can cut a day. 
 
 
 Thanks
 
 Sent from my iPhone
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[Histonet] RE: Non-GYN Prep Safety

[Podawiltz, Thomas]

Unfixed specimen should be done under a biological safety cabinet. 

Tom 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amanda Reichard
Sent: Wednesday, December 03, 2014 11:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Non-GYN Prep Safety

Question for you all

Do you process Non-GYN specimens under a hood?
What type of precautions are taken for suspect mycobacterium specimens?
Are your ThinPrep machines under a hood as well?

Thanks in advance

Amanda Reichard, HTL (ASCP)cm
Histotechnologist
Laboratory
Licking Memorial Health Systems
1320 W. Main St.
Newark, OH 43055
(740) 348-4157


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[Histonet] RE: Is there a Law for refusal of pathology services.

[Podawiltz, Thomas]

I would start with CAP/CLIA for their guidelines. Then check to see what the 
state regulations would be. 


Tom 


Tom Podawiltz HT (ASCP)
Histology Section Head 
LRGHealthcare
Laconia, NH 03246
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Tuesday, August 19, 2014 10:45 AM
To: Histonet
Subject: [Histonet] RE: Is there a Law for refusal of pathology services.

I believe the question was asking for laws. 

A law is different than what insurance companies may or may not prefer. They 
have 'rules' but have to follow 'laws'. Laws that might concern this are also 
enacted at both the national and state levels. Any state might differ over 
another.  All I can offer beyond that is, if really concerned, and it sounds 
like you are, take up the question with your Risk Management folks, or whatever 
department deals with that kind of stuff. 

This case is one you are aware of. It might shock you to learn how many times 
patients do not have their tissues examined, especially in clinics. The best 
record might the op-notes, as far as the insurance companies are concerned. 

To assume the patient won't be covered, that she has anterior motive, or that 
the lab is the sole documentation of a procedure is to make legal assumptions 
and, frankly, that's not a good idea. 

All of that said - it is, in my mind, prudent to get a diagnosis, but prudence 
is different than necessity and patient rights are pretty broad and are rarely 
narrowly interpreted. 

Thanks for reading this far down -

Bill (Not A Lawyer) O'Donnell

 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs
Sent: Tuesday, August 19, 2014 8:52 AM
To: Sue; Paula Pierce
Cc: Histonet
Subject: [Histonet] RE: Is there a Law for refusal of pathology services.

Also, legally speaking, if it isn't documented, it didn't happen in the eyes of 
the law.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu on behalf of Sue 
suetp...@comcast.net
Sent: Monday, August 18, 2014 9:43 PM
To: Paula Pierce
Cc: Histonet
Subject: Re: [Histonet] Is there a Law for refusal of pathology services.

I agree, if you do not document that a specimen was removed most likely 
insurance will deny the clain.

SPaturzo
TJU
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RE: [Histonet] On the lighter side...

[Podawiltz, Thomas]

29 years. 


Tom Podawiltz HT (ASCP)
Histology Section Head 
LRGHealthcare
Laconia, NH 03246
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter
Sent: Thursday, August 07, 2014 2:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] On the lighter side...

How long have you been a registered histotech?  36 years here.  You???

 

Douglas A. Porter, HT (ASCP) 
Grossing Technician 
IT Coordinator

Cancer Registrar 


CAP-Lab, PLC 
2508 South Cedar Street
Lansing, MI 48910-3138 

517-372-5520 (phone) 
517-372-5540 (fax) 

 mailto:doug.por...@caplab.org doug.por...@caplab.org 

 http://www.caplab.org/ www.caplab.org   

 

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[Histonet] RE: Re: Friday histology trivia

[Podawiltz, Thomas]

The navy lab I was stationed at in VA, we had a picture of Quincy on our dart 
board. Yeah, he was our favorite target. 

Tom 

-Original Message-
From: Shirley A. Powell [mailto:powell...@mercer.edu] 
Sent: Friday, June 27, 2014 2:22 PM
To: Podawiltz, Thomas; Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 
'Teri Johnson'; 'histonet@lists.utsouthwestern.edu'
Subject: RE: Re: Friday histology trivia

Most of you guys are too young to remember Quincy, who told his lab assistant 
that if he did not come up with an answer he would be demoted to the histology 
lab to count specimens.  Never watched that show again.  Good thing the writers 
were on the other side of the country at that time.  But hey I have mellowed 
since then.  All will agree that medical shows take license with truth and 
reality in view of the almighty $$$.  

Shirley   

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, 
Thomas
Sent: Friday, June 27, 2014 1:54 PM
To: Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 
'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Re: Friday histology trivia

The last medical show I watched was ER. Sent NBC an irritated e-mail after the 
episode where Dr. Wylie gave a resident a tube of blood and told her to take to 
the lab and wait there for the results since the lab loses everything. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Friday, June 27, 2014 1:30 PM
To: 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Re: Friday histology trivia

Remember the episode of House where the physicians assisting House dropped some 
red liquid on a slide and had an immuno?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Friday, June 27, 2014 1:25 PM
To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Re: Friday histology trivia

Are all news stories are as faked as the ones showing something in a lab? One 
had two doctors(?) in lab coats peering at a microscope slide they are holding 
to the light above their head and the reporter is saying they are examining 
samples from a cancer patient. 

Wow, good eyesight!

Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San 
Francisco Medical Center San Francisco, CA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson
Sent: Friday, June 27, 2014 10:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Friday histology trivia

Carefully placed scientific equipment, as well as spokespeople dressed in lab 
coats, are great marketing tools for anything touted to cure us or make us 
healthier.

Next time you watch an actual medical or research-based news story, check out 
how they ALWAYS show someone pipetting. Always.
It's become a game for me to spot it.

Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752

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[Histonet] RE: Re: Friday histology trivia

[Podawiltz, Thomas]

Oh man, how can I forget. Microtome grease and his cover's head band. 

Tom 

-Original Message-
From: O'Donnell, Bill [mailto:billodonn...@catholichealth.net] 
Sent: Friday, June 27, 2014 4:10 PM
To: Podawiltz, Thomas; Shirley A. Powell; Sanders, Jeanine (CDC/OID/NCEZID); 
'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu'
Subject: RE: Re: Friday histology trivia

Second favorite - don't forget Roger! - Bill

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, 
Thomas
Sent: Friday, June 27, 2014 2:11 PM
To: Shirley A. Powell; Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 
'Teri Johnson'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Re: Friday histology trivia

The navy lab I was stationed at in VA, we had a picture of Quincy on our dart 
board. Yeah, he was our favorite target. 

Tom 

-Original Message-
From: Shirley A. Powell 
[https://urldefense.proofpoint.com/v1/url?u=http://mailto:POWELL_SA%40mercer.eduk=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0Ar=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0Am=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0As=760f9835534c28a8d5a039ae8d8ca02cad445502b7e1ceb7dfe700a3d4a471e4]
 
Sent: Friday, June 27, 2014 2:22 PM
To: Podawiltz, Thomas; Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 
'Teri Johnson'; 'histonet@lists.utsouthwestern.edu'
Subject: RE: Re: Friday histology trivia

Most of you guys are too young to remember Quincy, who told his lab assistant 
that if he did not come up with an answer he would be demoted to the histology 
lab to count specimens.  Never watched that show again.  Good thing the writers 
were on the other side of the country at that time.  But hey I have mellowed 
since then.  All will agree that medical shows take license with truth and 
reality in view of the almighty $$$.  

Shirley   

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.eduk=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0Ar=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0Am=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0As=e0366327209d09783810b3f56489aa120062fa2a8c34700228309c97db308192]
 On Behalf Of Podawiltz, Thomas
Sent: Friday, June 27, 2014 1:54 PM
To: Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 
'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Re: Friday histology trivia

The last medical show I watched was ER. Sent NBC an irritated e-mail after the 
episode where Dr. Wylie gave a resident a tube of blood and told her to take to 
the lab and wait there for the results since the lab loses everything. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.eduk=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0Ar=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0Am=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0As=e0366327209d09783810b3f56489aa120062fa2a8c34700228309c97db308192]
 On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID)
Sent: Friday, June 27, 2014 1:30 PM
To: 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Re: Friday histology trivia

Remember the episode of House where the physicians assisting House dropped some 
red liquid on a slide and had an immuno?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.eduk=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0Ar=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0Am=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0As=e0366327209d09783810b3f56489aa120062fa2a8c34700228309c97db308192]
 On Behalf Of Morken, Timothy
Sent: Friday, June 27, 2014 1:25 PM
To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Re: Friday histology trivia

Are all news stories are as faked as the ones showing something in a lab? One 
had two doctors(?) in lab coats peering at a microscope slide they are holding 
to the light above their head and the reporter is saying they are examining 
samples from a cancer patient. 

Wow, good eyesight!

Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San 
Francisco Medical Center San Francisco, CA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.eduk=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0Ar=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0Am=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0As=e0366327209d09783810b3f56489aa120062fa2a8c34700228309c97db308192]
 On Behalf Of Teri Johnson
Sent: Friday, June 27, 2014 10:15 AM
To: histonet

[Histonet] RE: Recycled or not? NO PHI

[Podawiltz, Thomas]

We have never had an issue with either our recycled xylene or alcohol that was 
not self inflicted. When our system is running there are no fumes. 


Tom Podawiltz HT (ASCP)
Histology Section Head 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs
Sent: Thursday, June 26, 2014 9:06 AM
To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Recycled or not?

While I can agree that recycling alcohol and xylene is both environmentally and 
economically advantageous, technically it's awful.  There's no way to make used 
alcohol and xylene as pure as it was originally.  There's also the issue of 
fumes from recycling a solvent.  The company I had used years ago swore that 
there were no fumes when using their machine but the personnel working in the 
laboratory would vigorously disagree. 

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine 
(CDC/OID/NCEZID) j...@cdc.gov
Sent: Thursday, June 26, 2014 9:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Recycled or not?

Morning All!

I have heard for years the general problems with using recycled alcohols on HE 
stainers, but do the same problems occur when using recycled xylene?

Thanks!

Jeanine H. Sanders
Centers for Disease Control and Prevention Infectious Diseases Pathology Branch
404-639-3590
j...@cdc.gov


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[Histonet] RE: Recycled or not? NO PHI

[Podawiltz, Thomas]

They are very fussy as you put it. However, I am even more anal than they are 
about our work. We do QA checks both before and after we recycle. 



Tom Podawiltz HT (ASCP)
Histology Section Head 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220




-Original Message-
From: Barbara Tibbs [mailto:barbara.ti...@accuratediagnosticlabs.com] 
Sent: Thursday, June 26, 2014 10:37 AM
To: Podawiltz, Thomas; Sanders, Jeanine (CDC/OID/NCEZID); 
histonet@lists.utsouthwestern.edu
Subject: RE: Recycled or not? NO PHI

Maybe your pathologists aren't as fussy as the pathologists I worked with at 
the time.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: Podawiltz, Thomas tpodawi...@lrgh.org
Sent: Thursday, June 26, 2014 1:33 PM
To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); 
histonet@lists.utsouthwestern.edu
Subject: RE: Recycled or not?  NO PHI

We have never had an issue with either our recycled xylene or alcohol that was 
not self inflicted. When our system is running there are no fumes.


Tom Podawiltz HT (ASCP)
Histology Section Head
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs
Sent: Thursday, June 26, 2014 9:06 AM
To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Recycled or not?

While I can agree that recycling alcohol and xylene is both environmentally and 
economically advantageous, technically it's awful.  There's no way to make used 
alcohol and xylene as pure as it was originally.  There's also the issue of 
fumes from recycling a solvent.  The company I had used years ago swore that 
there were no fumes when using their machine but the personnel working in the 
laboratory would vigorously disagree.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine 
(CDC/OID/NCEZID) j...@cdc.gov
Sent: Thursday, June 26, 2014 9:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Recycled or not?

Morning All!

I have heard for years the general problems with using recycled alcohols on HE 
stainers, but do the same problems occur when using recycled xylene?

Thanks!

Jeanine H. Sanders
Centers for Disease Control and Prevention Infectious Diseases Pathology Branch
404-639-3590
j...@cdc.gov


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THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential 
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[Histonet] RE: Formalin in operating (surgery) rooms

[Podawiltz, Thomas]

We do not allow any formalin in the surgery rooms. They come to the Histology 
department to pre-fill their containers since they do not a fume hood in the 
OR. Those containers go into a utility room and a scrub tech will leave the 
surgical suite to place the collected specimen in the appropriate sized 
container. 

They had to start doing this several years ago after a spill in one the 
surgical suites. 


Tom 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candace J. 
Wagner
Sent: Thursday, June 12, 2014 1:50 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Formalin in operating (surgery) rooms

Hello all out in Histoland,
I had a surgery tech ask me if there was a specific amount of formalin allowed 
in the surgery rooms.
I could not find anywhere any documentation on a specific amount.  We supply 
our surgery dept. with the formalin they need, usually about 2 gallons in each 
room now, but just wondering if anyone has any idea if there is such a 
specific amount?? Thanks

-CJ-




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[Histonet] RE: IHC antibody optimizing validating

[Podawiltz, Thomas]

Don't even get me going. 

Tom 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Tuesday, March 25, 2014 10:28 AM
To: Davis, Cassie; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: IHC antibody optimizing  validating

Hi Cassie,

I have tried going 'requisition free a number of times in at least three 
institutions. The problems I've always encountered have been with the OR 
nursing staff, who frankly tell us that they are too busy to have to do one 
more thing - even if they were doing the 'thing' on paper anyway. 

(Now - for a little soapbox time) I have found that while there are a lot of 
hard working, diligent OR nurses, far too many see patient care as done once 
the tissue is out.  This means that many times, insufficient information or 
even - God help us - incorrect information ends up on the requisitions.  But it 
didn't get any better electronically, in fact it was worse. Having required 
fields before submission helps - but only if they bother to put something 
useful in them.  This may not be everyone's experience, in fact I hope it is 
not. My problem is not with paperless (which is never really paperless) but 
with trusting the people who do the ordering. 

Any way, thanks for letting me have a mini-rant. It felt good!

Shalom - Bill

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie
Sent: Tuesday, March 25, 2014 8:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC antibody optimizing  validating

Will you help me? I understand we are to use the known positives controls that 
the manufactures' recommends in the package insert when optimizing the stains, 
but I need to know what is your general procedure for optimizing (how many 
different staining protocols do you test) and validating a new antibody (how 
many different or known positive and negative tissues do you test [predictive 
markers I understand are 20])?

Cassandra Davis
cda...@che-east.org
302-575-8095



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RE: [Histonet] Soaking artifact

[Podawiltz, Thomas]

I agree Peggy. Just one question. What is a small histology lab to do when they 
only have one processor and cannot run separate cycles and do not have staffing 
to run short cycles throughout the day?



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee  Peggy Wenk
Sent: Monday, January 06, 2014 8:07 AM
To: Deanna Leslie; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Soaking artifact

The only soaking artifacts that I can think of would be caused by:
- soaking too long in water (minutes instead of a few seconds)
- soaking under-processed tissue in water

In both cases, the tissue is supposed to be protected by the wax, and if it 
is not (under-processed), or if the faced block is in water too long, the 
tissue can start re-absorbing water. The tissue then turns white and swells out 
of the block. So all that swelled out tissue is cut away and lost when the 
tissue is put back on the microtomy for sectioning the ribbon.

If you soak for just a few seconds, such as a gauze with water being held 
against the block on the microtome, after it has been faced, then you will get 
a little bit of water absorbed into just a few layers of cells. Just enough to 
cut 2-4 sections. And you won't see that swelling artifact.

For those of you saying - but I have to face all the blocks, put them back on 
ice and/or water while I cut a bunch more blocks, and then go back and cut each 
block - that is an artifact also. You have over-dehydrated your tissue during 
processing, and you are putting back the water that you should not have taken 
out. Processing is supposed to remove the unbound water (not attached to 
proteins), and some of the bound water (attached to proteins), and leave some 
of the bound water (attached to proteins) in the tissue. If you HAVE to soak 
EVERY block for more than a couple of seconds, then you are wasting time 
rehydrating and wasting time while microtoming. Cut down the time in the 
alcohols on the tissue processor, to leave a little bound water in the tissues. 
And you can NOT processing little biopsies on the same long processing cycle as 
the larger pieces of tissue (uterus, breast, etc.). 
Those little biopsies will be over-dehydrated. They HAVE to be run on a 
separate cycle of much shorter time intervals (10-20 minutes in each solution 
(once fixed), instead of 45-60 minutes in each solution).

You should be able (on nearly every tissue block) to rough trim the tissue, and 
immediately start cutting ribbons. Possibly, you will need to put an ice cube 
on some of the harder tissue (cervix, uterus, bone, lens, etc.) just to get the 
paraffin hardness to match the hardness of the tissue. That being said, some 
tissues are naturally brittle or crumbly, and always need some water put back 
in the tissue, such as spleen or bloody tissue, but again, some wet gauze on 
the faced block for a few seconds should be enough time to get 2-4 sections. 
And that's all the tissue we usually need from those blocks. If you need more 
for IHC, put the wet gauze back on the faced block, and cut a few more sections.

Peggy A. Wenk, HTL(ASCP)SLS

-Original Message-
From: Deanna Leslie
Sent: Sunday, January 05, 2014 4:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Soaking artifact

Has anybody in histoland ever heard of this?  I have been cutting tissue for 25 
yrs and until recently I had never heard of this!
I am under contract to a facility and the supervisor there does not want 
anybody to soak their tissue or use ice!  Your are supposed to use the cold 
plate, because as I have stated soaking them causing an artifact. I have not 
disputed this because it is not my place or in my job discription as a 
traveler.  I am not even sure what it is supposed to look like or what type of 
problems it causes.

Thanks for listening!
Deanna Leslie HT ASCP
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RE: [Histonet] RE: Yahoo link NO PHI

[Podawiltz, Thomas]

My current lab the air exchange rate is 58/hr.
The good news: you do not smell fumes of any kind.
The bad news: we are negative pressure and the unvented bathrooms are just 
outside our lab.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. 
Powell
Sent: Wednesday, December 04, 2013 11:42 AM
To: Weems, Joyce K.; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Yahoo link

I had the same experience, the clinical lab techs asked the supervisor to keep 
our door closed so they would not have to smell the fumes, just lock us up in 
it. I was considering calling EPA in to check it out.   Thank goodness those 
days are gone.  Use those fume hoods and all the other ppe you can get.  :)
Shirley Powell

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Wednesday, December 04, 2013 10:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Yahoo link

I worked in a non-ventilated lab once - back in the 70s - just before the 
xylene study was released. I knew something was wrong and I knew it was xylene 
and finally was able to get an exhaust put in the window . I could taste it, 
smell it, and seemed to cough it up!! And my brain was fuzzier than my usual 
fuzz! The lab director's office was next to my lab and she moved, because she 
said she couldn't stand to be next to me!

So thankful for good regs now and labs since then that have been very well 
ventilated. We have come a long way, baby!!

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Wednesday, December 04, 2013 9:52 AM
To: 'Lee  Peggy Wenk'; Elizabeth Cameron; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Yahoo link

I remember participating in the health study in the 80's.   Xylene and 
Formaldehyde levels are monitored in all labs.  If our hospitals/research 
centers would allow us to forward that information on to someone who could 
compile data it would be a starting place for a health study.

I strongly believe this profession can be danger to health.  I can name at 
least 10 histotechs who have died from cancer.  Yes, it may have been when they 
were older but I feel certain it was from all the chemical exposure in 
histology before safety became a priority.   I know others will remember no 
ventilation, smoking and eating in the lab, no real requirements for gloves, 
etc. There were also no MSDS information available and we were exposed 
through lack of knowledge.

I believe the lab is much safer today with all of the safety precautions we 
take and the knowledge we have on chemicals/stains and their toxicity.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee  Peggy Wenk
Sent: Wednesday, December 04, 2013 8:24 AM
To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Yahoo link

Couple of studies that I know of.

One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH 
Symposiums, and did different tests on people who volunteered to participate. 
Published findings in the late 1980's that said that histotechs had lower 
pulmonary function than average population, and decreased memory, equilibrium 
and dexterity than the general population.

In Letters to the Editor, people pointed out statistical flaws (low numbers of 
participants, for example). I also feel there were flaws, such as testing 
people after traveling over time zones, who were up late at the parties, and 
had possibly been drinking the night before. There was no way to measure how 
much exposure to formaldehyde or xylene people were really exposed to. I didn't 
participate, but if I though the amount I was being exposed to was medium, 
someone else being exposed to the same amount might have said low amount and 
someone else could have said high 

[Histonet] Microtome Blade safety, in or out when not in use? NO PHI

[Podawiltz, Thomas]

We do not re-use the blades or leave them in the microtome it is against our 
safety practices. We handle the blades twice, when we place them in the blade 
holder and when we remove them and drop them into the sharps container. 


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian
Sent: Friday, October 25, 2013 12:35 PM
To: Marcum, Pamela A; Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use?

AMEN TO THAT!

-Original Message-
From: Marcum, Pamela A [mailto:pamar...@uams.edu]
Sent: Friday, October 25, 2013 10:05 AM
To: McAnn, Sherrian; Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use?

The rule here is a blade is cheaper than a cut.  Anytime you are walking away 
and returning within a few minutes to cut use the knife guard otherwise throw 
the blade out.  It is an accident looking to happen.  Recently we had a tech 
decide not use the knife guard and seriously cut himself by misjudging the 
distance from his elbow reaching an knife he was no longer able to use.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian
Sent: Friday, October 25, 2013 8:46 AM
To: Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use?

I was taught that when leaving your microtome for any length of time to always 
take the blade out. We had a tech that had the habit of leaving the blade on 
her microtome and even though she had the safety guard up someone from  
biomed still  managed to lean on it and get cut (go
figure)
   If I want to save a slightly used blade to maybe trim with the next time 
then I will put it into a slide mailer (plastic with attached
lid) but that is a safety issue .  The safety officer says that when a blade is 
used and taken out then it should be thrown away..because the more it is being 
handled the greater the chance of injury. Just saying what they told me.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Friday, October 25, 2013 6:03 AM
To: Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Blade safety, in or out when not in use?

Left in but covered with the blade guard.  Not spanking new, but usable (for 
facing) get stored in an old box that the slides came in.  I like the slide 
mailer idea, and will switch to that.

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University Health 
System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P:  919.684.2091

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On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons
leah_simmon...@hotmail.comwrote:

 Hello all :-)
 I am doing a quick microtome blade safety survey, When you finish 
 work, do you leave your blade in the microtome behind the blade guard 
 or do you take it out?
 If you take it out and it is a new blade or a blade still useful for 
 trimming  where do you store it?
 Thank you for your feedback, I really appreciate it.
 Regards
 Leah Simmons

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RE: [Histonet] RE: bunsen burner at the embedding center

[Podawiltz, Thomas]

This thread reminds me of the time in the Navy, while assigned to the histology 
department full time we sometimes had to cover nights in hematology. I noticed 
the light on in the histology lab. I quietly opened the door to see one of the 
night chemistry techs smoking a joint while he stood next to our staining fume 
hood. I coughed and he tossed the joint into the sink. 
When asked by the chief if I was writing him up for getting stoned on duty I 
answered No I am writing him up for being stupid, he could of blown both of us 
up

Tom

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, September 20, 2013 10:09 AM
To: Weems, Joyce K.; 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: bunsen burner at the embedding center

 and it was always snowing and I had to walk 19 miles to work 
uphillboth ways. . We had to stock our cryostat with fresh cut blocks of 
ice from the river 10 miles away. (uphill, both ways) We had to start our 
Bunsen burner with steel and flint and we used our fingers instead of forceps.  
G. Gorden Liddy was a wimp compared to us 

Kids today don't know how good they got it! (They also don't know who G. Gordon 
Liddy was but can instinctively Google it) 

- Just some Friday fun for what has turned into a fun thread. - Oh wait, I 
forgot, this forum is for professional questions - so- Can you please 
unsubscribe me?

Have a great weekend! - Bill

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Friday, September 20, 2013 8:48 AM
To: 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: bunsen burner at the embedding center

With no ventilation

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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contain information that is privileged and confidential.  Any unauthorized 
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intended recipient, please delete this message, and reply to the sender 
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards, 
Richard E.
Sent: Friday, September 20, 2013 9:46 AM
To: 'Jim Burchette'; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: bunsen burner at the embedding center

And sit around smoking as  we  mounted the  sections from  xylene, in an open 
lab

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jim Burchette
Sent: 20 September 2013 14:41
To: Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: bunsen burner at the embedding center

Back in the 70's we would boil metal base molds in water using a bunsen burner 
and a 3 legged ring stand.
On Sep 20, 2013 9:37 AM, Davis, Cassie cda...@che-east.org wrote:

 Hi Valerie,
 When I started in Histo in 90' everybody used the alcohol 
 burners...Open flame concern became a concerned and the separate 
 forcep warmers were purchase because the old embedding centers did not 
 have the nice warmers like the new ones do. The last place I worked at 
 had an old embedding center when I started but we weren't allowed open flames.
 Fortunately, we found an unused Bacteria Incinerator that Micro.
 wasn't using and used that until that embedding center died. That worked 
 great!

 Cassandra Davis
 cda...@che-east.org
 302-575-8095


 From: Hannen, Valerie valerie.han...@parrishmed.com
 To: Histonet Post (histonet@lists.utsouthwestern.edu)  
 histonet@lists.utsouthwestern.edu
 Sent: Thursday, September 19, 2013 11:04 AM
 Subject: [Histonet] Bunsen Burner


 Hi all..

 We are having a discussion/ disagreement in our department as far as 
 whether using a bunsen burner at the embedding center is against fire codes.

 What is the consensus??


 Thanks,

 Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief 
 Parrish Medical Center
 951 N. Washington Ave.
 Titusville, Florida 32976
 Phone:(321) 268-6333 ext. 7506
 Fax: (321) 268-6149
 valerie.han...@parrishmed.com

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 Health East and is intended for the sole use of the intended 
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 unauthorized review, use, disclosure, or 

RE: [Histonet] Blade Rationing

[Podawiltz, Thomas]

Is your manager a Tech? Sure does not sound like one. Anyone that is willing to 
compromise the quality of diagnostic slides to save a dollar should not be in a 
management position. 


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teresa Moore
Sent: Monday, June 17, 2013 5:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blade Rationing

I work in a hospital, there are three of us on this particular shift and we cut 
approx. 200 blocks, give or take a few.  Our histo lab manager is telling us we 
should only be using one pack of blades (50 per pack) a month.  I'm wondering 
what other techs think of this especially lab managers and supervisors.

tmoor...@gmail.com
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RE: [Histonet] urine hemosiderin

[Podawiltz, Thomas]

We use ThinPrep. 



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson
Sent: Friday, May 17, 2013 9:39 AM
To: histonet
Subject: [Histonet] urine hemosiderin

What kind of slide preparation are you using for urine? Cytospin vs thin prep 
vial is the discussion we are having. And are you doing a manual or automated 
iron stain? Thanks for any info you can provide.



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com



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[Histonet] Histology Technician Opening.

[Podawiltz, Thomas]

As of May 24th I will have an opening for a Tech. Days only 40 hours per week. 
No weekends doing routine histology, no IHC.
Located in the beautiful Lakes Region of New Hampshire.

* If interested here is the link: LRGHealthcare | Career 
Centerhttp://www.lrgh.org/Jobs
www.lrgh.org/Jobs


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220

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RE: [Histonet] Waste Alcohol

[Podawiltz, Thomas]

Reagent Alcohol contains methanol, which is a U-listed EPA hazardous chemical 
and cannot be poured down the drain. 



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Tuesday, February 26, 2013 10:27 AM
To: 'Rene J Buesa'; Scott, Allison D; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Waste Alcohol

The ability to put alcohol down the drain is regulated by your wastewater 
utility.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, February 25, 2013 3:53 PM
To: Scott, Allison D; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Waste Alcohol

I always dumped the ethanol down the drain. I never recycled it because it 
takes almost 3 times more time that recycling xylene (3 times the cost) and the 
recycled alcohol is seldom of good quality.
When I stopped using xylene I had no more use (and exposure) when recycling.
René J.

From: Scott, Allison D allison.sc...@harrishealth.org
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Monday, February 25, 2013 3:27 PM
Subject: [Histonet] Waste Alcohol

Hello to all in histoland.  Is there anyone putting alcohol into a waste drum 
like for xylene.  We have a company that takes away our xylene.  Now the safety 
people are suggesting that we treat the alcohol the same way.  We have always 
poured the alcohol down the drain.  It seems very expensive.  Your help in this 
matter will be greatly appreciated.



Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287


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[Histonet] RE: Cassette labeling

[Podawiltz, Thomas]

That is basically what we do. 

Tom

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margiotta-Watz, 
Michele
Sent: Friday, February 08, 2013 7:55 AM
To: 'SimmsColon, Janine'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cassette labeling

We print out an embedding log and note the # of pieces/block from the Doc's 
dictation. We are small scale so it works fine for us.

Michele

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of SimmsColon, 
Janine
Sent: Friday, February 08, 2013 7:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette labeling

Good morning all, 

 

I hope all here in the East are prepared for the storm, (considering I am in 
New England and it is February it is not all that surprising to me yet I am 
always amazed at the commotion that occurs with every impending snow, but I 
digress). I know many out there may have cassette labelers so this may not be 
an issue but for those who still label by hand I am curious as to how you 
label. At my previous lab besides the case number (S13-accession number), we 
also indicated how to embed the pieces (= for on edge), and the number of 
pieces in the cassette. I am just curious as to how common this practice is or 
if there is any other way you annotate the number of pieces expected in a 
cassette. Thank you in advance for any feedback you provide as I have received 
many helpful responses to my previous posts.

 

Janine Simms Colon, BHA, CPhT, HT(ASCP)

Histology/Pathology

Johnson Memorial Hospital

201 Chestnut Hill Road

Stafford Springs, CT 06076

Office: 860-684-8230 ext. 5197

janine.simmsco...@jmmc.com

 


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RE: [Histonet] Re: Number of blocks

[Podawiltz, Thomas]

Yes, they would prefer quality cut sections over speed. They would rather wait 
for the slides than get junk. I do most of the final QA on the slides and will 
order any recuts for quality. It's a team approach to quality and when an issue 
does rise up, it's a group discussion on how to avoid it in the future instead 
of some of the finger pointing I have seen happen at other places. We are not 
perfect, but we are a good team. 

 
Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare


  


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Friday, October 26, 2012 12:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Number of blocks

As I asked before, do your pathologists have any input into any of this?

About embedding: I heard of a recently trained pathologist who, asked about an 
embedding problem, replied, What's embedding?

We spend thousands of dollars on a bronchoscopy or an EGD to get a tiny bit of 
tissue that contains a life-changing diagnosis. The specimen comes to the 
pathology lab and is grossed by a prosector who isn't allowed an embedding 
sheet. The embedder has no idea how many bits of tissue to look for. Then the 
microtomist is expected to cut 50 blocks an hour. Then the pathologist has to 
make a diagnosis on a venetian-blind section.

Good Management I'm sure. Bad medicine.

Bob Richmond
Samurai Pathologist
Maryville TN

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RE: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray

[Podawiltz, Thomas]

I was once worked at a place that had this sign on the wall that everyone saw 
as you entered the training room. 

When you think you are through learning, you are through here

Blows my mind when I run into people that refuse to learn anything current. 



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Saturday, September 29, 2012 7:12 PM
To: histotech...@gmail.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray


JennyYou don't need to respond to this, but I will post in case there is anyone 
else out there who is going through the same experiences and feeling 
discouraged.  There are many people in the field like this. I have been out 
there at least a little while and I went through the same response once out of 
histology school and get attitude all the time, still to this day, even though 
I have jumped through all the usual hoops at this point. I can tell you that 
while  working in some labs, I thought of quitting histology almost weekly 
because I got so sick of this kind of thing  People have gotten into the field 
in various ways,  and they sometimes get into ruts and they don't get out there 
much to learn all the new techniques and information. People coming in with 
ideas threaten the status quo,  and sometimes it is just difficult many 
people to change.  I haven't quit histology yet though,   and you shouldn't  
let other people drive you out or make you doubt yourself either. Trust me, we 
need educated and trained people in this field in a desperate way. Look, we all 
have stuff to learn,  new and old! If you stop learning and believe you know 
it all,  you are a real drag to everyone and holding back others who want to 
learn and grow. If you are a newer tech, I think that can be a plus. You are 
fresh and full of enthusiasm and new ideas. The seasoned can share what they 
have learned from time and experience, and you can bring new ideas and your 
fresh enthusiasm and energy- I don't see why that can't be a win-win. Anyhow, 
all of this are just my opinions, and I may get slammed for these comments like 
I have many times before,  but as far as I can tell  from what you have posted, 
 you are not wrong, and your techniques seem reasonable for the hospital 
clinical setting. In addition, you seem to understand why they work  for you 
and the technical rationale behind them. To me this is good. Many people have 
been taught how to do things, but not why.  We need more people who want to 
know why and who care about quality. Please read the other posts about blades ( 
they said it best already) but I feel that is crap about skimping  on them ( 
sorry) . No patient is worth less than any disposable blade. That is why they 
call them disposable. You should not waste supplies, that is irresponsible, but 
within reason you need decent supplies to get the best quality you can possible 
can. Ice is cheap, much cheaper than freeze it spray..(as someone mentioned and 
a good point) .. so there is no valid economic/operational reason there that I 
can see to justify any of that. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 Date: Sat, 29 Sep 2012 18:26:38 -0400
Subject: Re: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray
From: histotech...@gmail.com
To: joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu

Thanks everybody for your answers. I cant respond them all but I concluded that 
the best way to get good sections is too chill the blocks on ice because I 
agree that it facilitates the process. 

I really don't understand why my supervisor depends so much on freezing sprays 
to cut and the pathologist has never complained about artifacts caused by them 
but I do believe that they are present because I have seen them getting formed. 
It makes sectioning difficult because you try to get sections free of holes and 
that contributes to the problem. 


At my lab is the same thing. My supervisor is in charge of the embedding and 
she just use the ice only for hardening the paraffin block. We don't have a 
standard embedding center with cold plate. Since is a small lab we just have a 
heating plate where we handle the specimens and place them in the molds and we 
cool them on ice trays. After they are removed from the molds they are placed 
on the counter in numerical order and they reach room temperature and get warm. 
I do think that if they get cold and moistened since the beginning it can 
facilitate the sectioning process except for certain tissues that are not well 
processed. In my lab I change the reagents in the tissue processor weekly but 
they get dirty too quickly because we processes a lot of breast and colon 
tissue so is hard to get perfectly processed 

RE: [Histonet] Slide distribution amongst Pathologists

[Podawiltz, Thomas]

We have one simple way of doing it. He who Grosses is he who reads. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
susan.wal...@hcahealthcare.com
Sent: Tuesday, June 05, 2012 3:21 AM
To: gu.l...@gmx.at; sa...@hotmail.ca
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide distribution amongst Pathologists

Boy, they sure like to put us in the middle of what should be their own 
problem. Thank heavens I only work now for one Dr at a time now but when I was 
at a larger place they rotated. They still used to tell us to give them 
particular cases when it was not their turn so that we got the flak when 
someone did not get what they thought was theirs. You can never win! :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Monday, June 04, 2012 2:34 PM
To: 'Sheila Adey'
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Slide distribution amongst Pathologists

Here the slides go through the hands of one pathologist, who distributes the
cases. 
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sheila
Adey
Gesendet: Montag, 04. Juni 2012 20:19
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Slide distribution amongst Pathologists


Hello Netters:
I am looking for some ideas regarding slide distribution amongst
pathologists.
Currently one Dr. reads all the surgicals and one other reads the cytology
and bone marrows.
We now have 5 Dr.s and they are looking for ways to disperse the work evenly
per day.
Thanks
:)
 
Sheila Adey
Charge Technologist
Laboratory - Histology Department
Bluewater Health
89 Norman Street
Sarnia, ON  N7T 6S3
519-464-4500 x 7063
 
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RE: [Histonet] (no subject)

[Podawiltz, Thomas]

The last person I hired for a tech position, was just finishing her on-line 
program. I helped her complete that and then she was given two years to get her 
certification. This was all part of her hiring agreement, so she knew this 
coming in and completely understood that failing to achieve either one would 
affect her employment.



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
 
 

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Tuesday, May 22, 2012 1:43 PM
To: sherrian.mc...@va.gov; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)


That seems to be the unfortunate situation at this time...




Joelle Weaver MAOM, HTL (ASCP) QIHC
  Date: Tue, 22 May 2012 12:39:39 -0500
 From: sherrian.mc...@va.gov
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] (no subject)
 
 I agree and would like to add.  This is one scenario that I have seen
 many times, where hospitals  or wherever will hire histotechs without
 certifications .  I am thinking that saves them money and they still
 have a histotech.  I have seen good histotechs  that have no
 certification and likewise some bad ones with certification.  Lately I
 have seen these  schools turn out histotechs   ,  it seems with little
 encouragement to get certified.  If places will hire them without being
 certified,  there seems little incentive (unless you are self motivated
 for more money) to move on up to certification.
 
  
 
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[Histonet] RE: Flammable cabinets

[Podawiltz, Thomas]

Depends of the amount the cabinet is rated for. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Tuesday, April 10, 2012 4:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Flammable cabinets


Can anybody explain how much alcohol or other flammables we can store in a 
flammable cabinet in a room?  I have read the CAP guidelines and am still 
confused.  Do the CAP guidelines  only have to do with stored reagents outside 
of a flammable cabinet?
What am I missing?

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



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RE: [Histonet] FW: Redneck Lent

[Podawiltz, Thomas]

I help run an online first person shooter game server and one of the few rules 
we have is you need a thick skin to play here. 
The other few rules are No Racism, politics or religion discussions while 
playing. Come to have fun. 

To me the same should apply here. I come here for advice, sometimes give some, 
but mostly to learn. Work related jokes are fine, but I think we should avoid 
the hot buttons. Just stating my opinion.  


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette
Sent: Tuesday, March 27, 2012 10:09 AM
To: 'Davide Costanzo'; JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA 
FRAZEE; mike  tony siltman
Subject: RE: [Histonet] FW: Redneck Lent

For goodness sakes--lighten up.  It was a funny 'joke' and I didn't see 
anything to get so upset about. I do see that if everyone did it then it could 
disrupt what 'Histonet' is supposed to be about so I guess any joke telling or 
anything not work related is inappropriate

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo
Sent: Monday, March 26, 2012 6:56 PM
To: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike  tony 
siltman
Subject: RE: [Histonet] FW: Redneck Lent

Religious humor on this listserv is remarkably inappropriate. I cannot believe 
anyone would post this here. Tasteless.

Sent from my Windows Phone
From: JOSEPH FRAZEE
Sent: 3/26/2012 3:49 PM
To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike  tony siltman
Subject: [Histonet] FW: Redneck Lent


 Date: Mon, 26 Mar 2012 19:55:27 +0100
From: spoeri...@yahoo.com
Subject: Fw: Fwd: Redneck Lent
To: karen.green...@hobbylobby.com; stewartdap...@hotmail.com; 
yvette.fette...@basf.com; footch...@yahoo.com; frazeeli...@hotmail.com; 
jfra...@hotmail.com; donna.lu...@gmail.com




Kerri



- Forwarded Message -
From: Sharen Pray praysha...@yahoo.com
To: Ruth Posey ruthalpo...@yahoo.com; LueAnn Root lar...@ymail.com; 
Marjorie Norris nursenor...@yahoo.com; Tom Voss, Sr. 
tomv...@wildblue.net; Taber Stewart texcon.ta...@gmail.com; MONTIE L 
WINTERS molo...@yahoo.com; Terry Maloney maloney_te...@yahoo.com; kerri 
spoering spoeri...@yahoo.com; Kenny  Debbie Hager kanddha...@att.net
Sent: Saturday, 24 March 2012, 21:06
Subject: Fw: Fwd: Redneck Lent


















Each Friday night after work, Bubba would fire up his outdoor grill and cook a 
venison steak. But, all of





Bubba's neighbors were Catholic. And since it was Lent, they were forbidden 
from eating meat on Friday.


The delicious aroma from the grilled venison steaks was causing such a problem 
for the Catholic faithful that they finally talked to their priest.

The Priest came to visit Bubba, and suggested that he become a Catholic.

After several classes and much study, Bubba attended Mass...and as the priest 
sprinkled holy water over him, he said, You were born a Baptist, and raised a 
Baptist, but now you are a Catholic.

Bubba's neighbors were greatly relieved, until Friday night arrived, and the 
wonderful aroma of grilled venison filled the neighborhood.

The Priest was called immediately by the neighbors, and, as he rushed into 
Bubba's yard, clutching a rosary and prepared to scold him, he stopped and 
watched in amazement.

There stood Bubba, clutching a small bottle of holy water which he carefully 
sprinkled over the grilling meat and chanted:
You wuz born a
 deer, you wuz raised a deer, but now you is a catfish.



Blessings, love and light, Live simply, love generously, care deeply, speak 
kindly.





  
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RE: [Histonet] re: April fools prank

[Podawiltz, Thomas]

The one that almost got us in a lot of trouble was the one that we had everyone 
convinced that I had been TDY'd to Quantico instead of going on leave. The best 
part about that one, was while the Officers in charge were running around 
trying to figure out who changed my orders from leave to TDY, no one thought to 
go to Sue's lab and ask her were I really was. The only thing that kept us from 
getting in trouble was none of the officers wanted to explain to the base 
Admiral why the fell for the joke. I still laugh when I think about that. 


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: O'Donnell, Bill [mailto:billodonn...@catholichealth.net] 
Sent: Thursday, March 22, 2012 2:40 PM
To: Podawiltz, Thomas; Kara Lee; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] re: April fools prank

Tom,

Not always a simple, fun prank, but bordering on the, I don't
know..psycopathic? (I won't mention them, as someone might actually
do them, then I'd have all those court appearences to deal with and
phycological evaluations I just don't have that kind of time
anymore) Of all the stunts we pulled, the ONLY one that ever got me in
trouble was putting carbonated water in someones waterbath. Certainly
one of the more benign efforts. I will likely do a lot of time in
purgatory for some of those, mostly because they still cause me to
smile, even after all these years. And to think we have become respected
professionals and pillars of society. Who'd-a-thunk it?  - Bill

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Podawiltz, Thomas
Sent: Thursday, March 22, 2012 1:20 PM
To: Kara Lee; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] re: April fools prank

I used to be real bad about pulling pranks in my younger days. If fact,
there were three of use in our Navy lab that pranked each other all the
time. Then to the horror of the rest of the staff we joined forces and
starting pranking everyone else. It finally got to the point that all we
had to do was make a comment about something and watch everyone's
paranoia go up. Funny thing was on April first we never did a
thing...and that drove the staff nuts. 



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee
Sent: Thursday, March 22, 2012 11:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] re: april fools prank




One must always consider who the prank is being pulled on.  If the
person is sensitive or not quite in line with your own Humor a prank
should not be pulled.  As people working in the science industry, we
have all been trained to observe.  If a person is not the type to deal
well with that type of humor, don't bother them and let them be.  Or,
ask if they want to be included in playing a prank on someone else who
lives for seeing what their co-workers are doing to them next..I had a
co-worker who was bummed when we were all too busy to mess with each
others stuff one April 1st one year. 

Pranks and jokes should always make the other person laugh or smile just
as much as you do, and don't forget to help them clean up the mess you
made...but I think we all know that, right? ;)

Enjoy life with your co-worker friends, and laugh! I mean, we do spend
over 50% of our lives with these people.

Cheers everyone!
Kara 


 From: s...@stowers.org
 To: hmarlat...@gmail.com; histonet@lists.utsouthwestern.edu
 Date: Thu, 22 Mar 2012 07:14:41 -0500
 Subject: RE: [Histonet] re: april fools prank
 CC: 
 
 Heather, I see no harm in a fake spider.  That would be fun.  But, I
totally agree with what Barry just wrote.  Some of these other pranks
are out of line, in my opinion.  I'm all for having fun and enjoying the
spirit of the day, but I don't believe in aggravating or embarrassing
someone for your satisfaction.  I have also pulled the hot dog trick on
a resident pathologist.  He loved it. 
 Sharon
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
 heather marlatt
 Sent: Wednesday, March 21, 2012 10:25 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] re: april fools prank
 
 I've been known to leave a fake spider in an embedder for the morning 
 person.although it wasn't april fools just for fun it got a great 
 reaction :) ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 ___
 Histonet mailing list
 Histonet

RE: [Histonet] re: April fools prank

[Podawiltz, Thomas]

So true, the person who found it the funniest was the Master Chief at 
Personnel, once he told me the whole story of what happened and wiped the laugh 
tears from his eyes. He warned me to keep a very, very, very low profile for 
awhile, which I passed on to you, Jesse and the rest of our crew. 

-Original Message-
From: O'Donnell, Bill [mailto:billodonn...@catholichealth.net] 
Sent: Friday, March 23, 2012 10:40 AM
To: Podawiltz, Thomas; Kara Lee; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] re: April fools prank

We were safe as long as their egos were intact. 

-Original Message-
From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org] 
Sent: Friday, March 23, 2012 9:34 AM
To: O'Donnell, Bill; Kara Lee; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] re: April fools prank

The one that almost got us in a lot of trouble was the one that we had
everyone convinced that I had been TDY'd to Quantico instead of going on
leave. The best part about that one, was while the Officers in charge
were running around trying to figure out who changed my orders from
leave to TDY, no one thought to go to Sue's lab and ask her were I
really was. The only thing that kept us from getting in trouble was none
of the officers wanted to explain to the base Admiral why the fell for
the joke. I still laugh when I think about that. 


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: O'Donnell, Bill [mailto:billodonn...@catholichealth.net]
Sent: Thursday, March 22, 2012 2:40 PM
To: Podawiltz, Thomas; Kara Lee; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] re: April fools prank

Tom,

Not always a simple, fun prank, but bordering on the, I don't
know..psycopathic? (I won't mention them, as someone might actually
do them, then I'd have all those court appearences to deal with and
phycological evaluations I just don't have that kind of time
anymore) Of all the stunts we pulled, the ONLY one that ever got me in
trouble was putting carbonated water in someones waterbath. Certainly
one of the more benign efforts. I will likely do a lot of time in
purgatory for some of those, mostly because they still cause me to
smile, even after all these years. And to think we have become respected
professionals and pillars of society. Who'd-a-thunk it?  - Bill

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Podawiltz, Thomas
Sent: Thursday, March 22, 2012 1:20 PM
To: Kara Lee; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] re: April fools prank

I used to be real bad about pulling pranks in my younger days. If fact,
there were three of use in our Navy lab that pranked each other all the
time. Then to the horror of the rest of the staff we joined forces and
starting pranking everyone else. It finally got to the point that all we
had to do was make a comment about something and watch everyone's
paranoia go up. Funny thing was on April first we never did a
thing...and that drove the staff nuts. 



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee
Sent: Thursday, March 22, 2012 11:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] re: april fools prank




One must always consider who the prank is being pulled on.  If the
person is sensitive or not quite in line with your own Humor a prank
should not be pulled.  As people working in the science industry, we
have all been trained to observe.  If a person is not the type to deal
well with that type of humor, don't bother them and let them be.  Or,
ask if they want to be included in playing a prank on someone else who
lives for seeing what their co-workers are doing to them next..I had a
co-worker who was bummed when we were all too busy to mess with each
others stuff one April 1st one year. 

Pranks and jokes should always make the other person laugh or smile just
as much as you do, and don't forget to help them clean up the mess you
made...but I think we all know that, right? ;)

Enjoy life with your co-worker friends, and laugh! I mean, we do spend
over 50% of our lives with these people.

Cheers everyone!
Kara 


 From: s...@stowers.org
 To: hmarlat...@gmail.com; histonet@lists.utsouthwestern.edu
 Date: Thu, 22 Mar 2012 07:14:41 -0500
 Subject: RE: [Histonet] re: april fools prank
 CC: 
 
 Heather, I see no harm in a fake spider.  That would be fun.  But, I
totally agree with what Barry just wrote.  Some of these other pranks
are out of line, in my opinion.  I'm all for having fun and enjoying the
spirit of the day, but I don't believe in aggravating or embarrassing
someone for your

RE: [Histonet] re: April fools prank

[Podawiltz, Thomas]

I used to be real bad about pulling pranks in my younger days. If fact, there 
were three of use in our Navy lab that pranked each other all the time. Then to 
the horror of the rest of the staff we joined forces and starting pranking 
everyone else. It finally got to the point that all we had to do was make a 
comment about something and watch everyone's paranoia go up. Funny thing was on 
April first we never did a thing...and that drove the staff nuts. 



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee
Sent: Thursday, March 22, 2012 11:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] re: april fools prank




One must always consider who the prank is being pulled on.  If the person is 
sensitive or not quite in line with your own Humor a prank should not be 
pulled.  As people working in the science industry, we have all been trained to 
observe.  If a person is not the type to deal well with that type of humor, 
don't bother them and let them be.  Or, ask if they want to be included in 
playing a prank on someone else who lives for seeing what their co-workers are 
doing to them next..I had a co-worker who was bummed when we were all too busy 
to mess with each others stuff one April 1st one year. 

Pranks and jokes should always make the other person laugh or smile just as 
much as you do, and don't forget to help them clean up the mess you made...but 
I think we all know that, right? ;)

Enjoy life with your co-worker friends, and laugh! I mean, we do spend over 50% 
of our lives with these people.

Cheers everyone!
Kara 


 From: s...@stowers.org
 To: hmarlat...@gmail.com; histonet@lists.utsouthwestern.edu
 Date: Thu, 22 Mar 2012 07:14:41 -0500
 Subject: RE: [Histonet] re: april fools prank
 CC: 
 
 Heather, I see no harm in a fake spider.  That would be fun.  But, I totally 
 agree with what Barry just wrote.  Some of these other pranks are out of 
 line, in my opinion.  I'm all for having fun and enjoying the spirit of the 
 day, but I don't believe in aggravating or embarrassing someone for your 
 satisfaction.  I have also pulled the hot dog trick on a resident 
 pathologist.  He loved it. 
 Sharon
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of heather 
 marlatt
 Sent: Wednesday, March 21, 2012 10:25 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] re: april fools prank
 
 I've been known to leave a fake spider in an embedder for the morning 
 person.although it wasn't april fools just for fun it got a great 
 reaction :) ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 ___
 Histonet mailing list
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 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  
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RE: [Histonet] Re: Does xylene cause skin cancer?

[Podawiltz, Thomas]

I was sent this link a couple of weeks ago, thought I would share it. 

http://www.darkdaily.com/health-of-pathology-laboratory-technicians-at-risk-from-common-solvents-like-xylene-and-toluene-070511#axzz1hkVgUzSf



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, February 24, 2012 11:48 AM
To: histonet@lists.utsouthwestern.edu; Jerry Ricks
Subject: Re: [Histonet] Re: Does xylene cause skin cancer?

There is an extensive literature linking xylene with some types of cancers, 
especially blood cancers, as well as with birth defects caused by it and many 
other aromatic compounds.
The real solution is to just eliminate it, and that be done from ALL, and I 
mean ALL, the histology phases where it is used now. That is what I did.
René J.

--- On Thu, 2/23/12, Jerry Ricks rosenfeld...@hotmail.com wrote:


From: Jerry Ricks rosenfeld...@hotmail.com
Subject: [Histonet] Re: Does xylene cause skin cancer?
To: histonet@lists.utsouthwestern.edu
Date: Thursday, February 23, 2012, 5:31 PM



As far as I know Xylene is not a conformed carcinogen.  On the other hand the 
structure is close to that of Benzene which is a confirmed human carcinogen.  
It's an aromatic hydrocarbon, so why take chances.  It makes sense to 1) 
minimize use. 2) Use fume hoods when possible.  3) Wear PPE--nitrile gloves not 
latex!




http://en.wikipedia.org/wiki/Xylene



http://www.ccohs.ca/oshanswers/chemicals/chem_profiles/xylene/health_xyl.html



http://www.ccohs.ca/oshanswers/chemicals/chem_profiles/benzene/health_ben.html#_1_6




Jerry Ricks
Research Scientist
University of Washington
Department of Pathology



 Date: Thu, 23 Feb 2012 13:33:00 -0500
 From: rsrichm...@gmail.com
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Re: Does xylene cause skin cancer?
 
 What's badly needed is a registry of death certificates of
 histotechnologists. The thing I'd want to examine would be the
 prevalence of myeloid leukemia and related diseases - known to be
 elevated in workers exposed to benzene. If such a correlation were
 found, it would mandate eliminating xylene from histology and cytology
 labs, and increasing precautions for handling resinous mounting media.
 
 The American Medical Association maintains such a registry of American
 physicians (or used to). It was used to establish that exposure to
 ionizing radiation (fluoroscopes and other X-ray equipment) was
 correlated with deaths from myeloid leukemia. Pathologists didn't have
 any particular problems - here I'd wonder about formaldehyde exposure
 and upper airway cancer. Pathologists get more exposure to
 formaldehyde than do histotechnologists.
 
 Bob Richmond
 Samurai Pathologist
 Knoxville TN
 
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RE: [Histonet] Union positions?

[Podawiltz, Thomas]

Union position? Only if I was starving. 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Bakken
Sent: Friday, February 17, 2012 10:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Union positions?

Trying to put together some numbers, any and all responses to these questions 
would be greatly appreciated.  How many HT's are in union positions?  If you 
were applying for a position, how much of a factor would it be if it was a 
union position?
 
Thanks in advance for your help!

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[Histonet] RE: Breast IHC testing

[Podawiltz, Thomas]

This abstract is in the January 2012 edition of CAP Today. 

Effect of prolonged fixation on evaluation of ER, PR, and HER2 expression in 
breast cancer 

Expression of estrogen receptor, progesterone receptor, and human epidermal 
growth factor receptor 2 is important in predicting a response to targeted 
therapies in breast cancer. Therefore, immunohistochemical assays to determine 
hormone receptor (HR) and human epidermal growth factor receptor 2 (HER2) 
status must be accurate and reproducible. Tissue fixation has been shown to 
play a crucial role in determining consistency in quality. Although guidelines 
impose upper limits for the fixation period, the data on which these limits are 
based are scant. The authors conducted a study to prospectively examine the 
effect of fixation of longer than 72 hours on these assays. In 101 invasive 
breast cancer samples, HR and HER2 status were compared between tumor blocks 
undergoing a short fixation period and those undergoing a period of prolonged 
fixation. Discordances were classified as an incremental change between 
categories of (i) a single order of magnitude-that is, a difference in the 
status of low positive (Allred score, 3) compared with positive (Allred score, 
4 to 8) or negative (Allred score, 0 or 2) and vice versa for HRs and a 
difference in HER2 status of equivocal compared with negative or positive and 
vice versa or (ii) greater than a single order of magnitude-that is, a 
difference in the status of positive compared with negative or vice versa. The 
median fixation time for the short fixation group was 13 hours and 18 minutes 
(mean, 13 hours and 17 minutes; range, 10 hours and 33 minutes to 17 hours and 
45 minutes) and for the prolonged fixation group was 79 hours and 22 minutes 
(mean, 79 hours and 35 minutes; range, 73 hours and 33 minutes to 102 hours and 
30 minutes). Eight cases showed discordances, all of which were of a single 
order of magnitude, including one for ER, five for PR, and two for HER2. In six 
of these, a higher score was seen in the prolonged fixation group. The authors 
concluded that fixation for limited periods beyond 72 hours does not reduce 
assay sensitivity in determining ER, PR, or HER2 immunohistochemical status. 

Tong LC, Nelson N, Tsourigiannis J, et al. The effect of prolonged fixation on 
the immunohistochemical evaluation of estrogen receptor, progesterone receptor, 
and HER2 expression in invasive breast cancer: a prospective study. Am J Surg 
Pathol. 2011;35:545-552.

Correspondence: Anna Marie Mulligan at mullig...@smh.ca
[ Top ]


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220




Anatomic pathology abstracts editors: Michael Cibull, MD, professor and vice 
chair, Department of Pathology and Laboratory Medicine, University of Kentucky 
College of Medicine, Lexington; Rouzan Karabakhtsian, MD, assistant professor 
of pathology and laboratory medicine, University of Kentucky College of 
Medicine; and Thomas Cibull, MD, dermatopathologist, Evanston Hospital, 
NorthShore University HealthSystem, Evanston, Ill.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Friday, February 03, 2012 11:14 AM
To: 'Gale Limron'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Breast IHC testing

Remember that you can validate your testing for longer fixation. Run parallel  
ER/PR/Her2 testing with tissue fixed for various lengths of  times and look at 
the staining correlation between groups. Most likely your longer fixation won't 
be a problem.  If you do see a drop off with longer times you can probably 
adjust antigen retrieval time to alleviate that.

Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gale Limron
Sent: Friday, February 03, 2012 7:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Breast IHC testing

I would like to know what other hospitals are doing with breast specimens that 
are resected on Friday and are in formalin longer than the maximum number of 
hours that CAP allows for ER/PR  and HER2/neu testing. We are running into this 
problem since we don't currently work Saturday hours.
Thank you!
Gale Limron CT,HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562



This e-mail is intended only for the person or entity to which it is addressed 
and may contain information that is privileged, confidential or otherwise 
protected from disclosure. Dissemination, distribution or copying of this 
e-mail or the information herein by anyone other than the intended recipient, 
or an 

RE: [Histonet] Music in the Laboratory

[Podawiltz, Thomas]

I have always allowed music to play at work usually left on a radio station 
since it will keep us updated on the day's information and leave the more 
offensive language out. I bought the department a system that will play cd and 
iPods'. The only rule is while playing our iPods', the songs with the strong 
language must be skipped. 


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang 
[gu.l...@gmx.at]
Sent: Wednesday, January 18, 2012 5:12 AM
To: tracz...@aol.com
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Music in the Laboratory

It would be fine, if there could be found a compromise. We have the radio on
during cutting time, but it is quiet enough not to overblow work. And in
the minutes nobody talks it is quite amousing to listen daily infos and
music.
And yes, also for me it would be a pain to listen to hard music at work
all the time.
On the other hand, a pathologist in our department loves classic music at
the microscope. - that's also too much for me. ;)

Gudrun


-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von
tracz...@aol.com
Gesendet: Mittwoch, 18. Jänner 2012 03:37
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Music in the Laboratory

Greetings.
I would like to know what other histology laboratories allow for music
players while working.  Do you have formal policies about music content or
volume?  Do you allow lab space doors to remain closed to muffle the volume
of
what is being played?  Are headsets allowed?
I am a terrible judge of this because I personally prefer to work in a
quiet environment.  I am trying to be open minded, as long as the work gets

done.  However, one of the techs had a song playing today that I  believe
was
inappropriate for general listening in the lab.  Am I just out  of touch?
Is that dang F word just something I'm going to have to learn  to accept?

Do you have a written policy?  When/how/why was it  implemented?
I should mention that it's a small private lab, with minimal patient
traffic.  We do see our share of FedEx, UPS, sales  service  reps.
Your ideas on this is very much appreciated.
Dorothy
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RE: [Histonet] Respirators and Routine Histology

[Podawiltz, Thomas]

We have our fume hoods inspected twice per year and the air exchange rate in 
our room is 52 times per hour, so we do not wear respirators in the lab. We 
only wear the full face respirators in the hazardous waste storage are while we 
are transferring the waste into the storage containers. 

We do wear the xylene and formalin badges for testing once per year then have 
the results sent to employee health. 



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
jsjurc...@comcast.net
Sent: Thursday, January 05, 2012 3:31 PM
To: Linda Blazek
Cc: histonet@lists.utsouthwestern.edu; Amy Self
Subject: Re: [Histonet] Respirators and Routine Histology

They must be in a liberal part of Michigan. 

- Original Message -
From: Linda Blazek lbla...@digestivespecialists.com 
To: Lisa Brenner li...@hollandhospital.org, Amy Self 
as...@georgetownhospitalsystem.org, histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu 
Sent: Thursday, January 5, 2012 2:30:20 PM 
Subject: RE: [Histonet] Respirators and Routine Histology 

Nothing at all??? 

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lisa Brenner 
Sent: Thursday, January 05, 2012 3:22 PM 
To: Amy Self; 'histonet@lists.utsouthwestern.edu' 
Subject: Re: [Histonet] Respirators and Routine Histology 

No, not here. We don't wear anything at all. We have excellent air flow, and we 
wear the badges that test for exposure to xylene and formalin annually which I 
think is a CAP requirement. Our results have been better than acceptable every 
year. 


Lisa Brenner HTL (ASCP) 
Histology Technical Consultant 
Holland Hospital 
phone: (616)394-3184 
li...@hollandhospital.org Amy Self as...@georgetownhospitalsystem.org 
1/5/2012 3:09 PM  Happy New Year to All, 

I need some help from all of you out there in histoland. 

How many of you wear respirators during your entire 8 hour work day for routine 
histology? If you don't wear a respirator do you wear any type of mask or 
shield at all for routine histology? 

Also if any of you have any histology safety procedures or information that you 
would be willing to share with me I would greatly appreciate it. 

Thanks in advance for all of your help, Amy 


Amy Self 
Georgetown Hospital System 
843-527-7179 
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RE: [Histonet] Chemical Recycling - B/R Instruments

[Podawiltz, Thomas]

Have never had a problem with ours. 


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo
Sent: Monday, November 21, 2011 12:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Chemical Recycling - B/R Instruments

Can anyone provide me information on B/R Instruments Pro series recyclers?
I am considerning them vs. CBG Biotech. I have heard rumors that the B/R
units throw a lot of heat from the lower boiler and damage the underlying
flooring. Can anyone confirm this, or any other troubles with their units?
Thank you.

-- 
*David Costanzo, MHS, PA (ASCP)*
Project Manager
*Blufrog Path Lab Solutions*
9401 Wilshire Blvd. Ste 650
Beverly Hills, CA 90212
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RE: [Histonet] SALARY

[Podawiltz, Thomas]

I like your world. 
I have seen more places that try to price Histology in the same range as CLA's 
not even close to MLT's. 

Tom

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Friday, October 28, 2011 12:52 PM
To: kcasti...@frii.com; Histonet@lists.utsouthwestern.edu; Caroline Pratt
Subject: RE: [Histonet] SALARY

I think that's low.  If you find a good candidate with years of experience I 
would pay them whatever it takes to get them in the door.  It's like Free 
Agency in baseball; if you want a good player, you need to put the money on 
table.  Histotechnologists are the most valuable employees in the laboratory 
today! 

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


 Pratt, Caroline caroline.pr...@uphs.upenn.edu 10/28/2011 9:21 AM 
I would say $28 to $32 dollars an hour depending on experience and education.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
kcasti...@frii.com 
Sent: Friday, October 28, 2011 7:54 AM
To: Histonet@lists.utsouthwestern.edu 
Subject: [Histonet] SALARY

HI EVERYONE,

WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID 
THESE DAYS.  HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM 
LAB AND DOING MOHS ALSO.  THANKS FOR YOUR HELP.  KRISTY

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RE: [Histonet] Ann Preece (was decal [sic] question)

[Podawiltz, Thomas]

I had a dachshund named Willey, he ate my copy, guess he like the pictures.


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Tuesday, October 04, 2011 1:23 PM
To: Bob Richmond; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Ann Preece (was decal [sic] question)

Ah, yes. That edition featured 105 illustrations, including 2
four-color plates (back in the day when that kind of stuff was worth
mentioning on the title page) 

Have a great day - Bill
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob
Richmond
Sent: Tuesday, October 04, 2011 10:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ann Preece (was decal [sic] question)

Bernice Frederick HTL (ASCP), Senior Research Tech at the  Pathology
Core Facility of the  Robert. H. Lurie Cancer Center at Northwestern
University in Chicago notes

Ann Preece states acid decal uses aqueous solutions of either formic, 
nitric, or trichloroacetic acid. Other methods mentioned are 
Ion-exchange resin, electrical ionization and chelation. The histo 
bible!

You've got to be almost as geezer as me to remember when Ann Preece's A
Manual for Histologic Technicians was the histo bible. I was fortunate
to be able to purloin a pristine (no stain spills) copy of the third
edition (1972) from the wreckage of an old histology lab about 20 years
ago.

Indeed, Patsy Ruegg! Decal is a trademark of the Decal Chemical
Corporation and should not be used generically for decalcifying
solutions. See decal-bone.com

Bob Richmond
Samurai Pathologist
Knoxville TN

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RE: [Histonet] Ann Preece (was decal [sic] question)

[Podawiltz, Thomas]

Yes he was, and yes he did



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: O'Donnell, Bill [billodonn...@catholichealth.net]
Sent: Tuesday, October 04, 2011 2:29 PM
To: Podawiltz, Thomas; Bob Richmond; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Ann Preece (was decal [sic] question)

Was Willey the dog you and sue had in VA? Seems he ate everything -
including an electrical cord -

-Original Message-
From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org]
Sent: Tuesday, October 04, 2011 1:27 PM
To: O'Donnell, Bill; Bob Richmond; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Ann Preece (was decal [sic] question)

I had a dachshund named Willey, he ate my copy, guess he like the
pictures.


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
O'Donnell, Bill
Sent: Tuesday, October 04, 2011 1:23 PM
To: Bob Richmond; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Ann Preece (was decal [sic] question)

Ah, yes. That edition featured 105 illustrations, including 2
four-color plates (back in the day when that kind of stuff was worth
mentioning on the title page)

Have a great day - Bill


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob
Richmond
Sent: Tuesday, October 04, 2011 10:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ann Preece (was decal [sic] question)

Bernice Frederick HTL (ASCP), Senior Research Tech at the  Pathology
Core Facility of the  Robert. H. Lurie Cancer Center at Northwestern
University in Chicago notes

Ann Preece states acid decal uses aqueous solutions of either formic,
nitric, or trichloroacetic acid. Other methods mentioned are
Ion-exchange resin, electrical ionization and chelation. The histo
bible!

You've got to be almost as geezer as me to remember when Ann Preece's A
Manual for Histologic Technicians was the histo bible. I was fortunate
to be able to purloin a pristine (no stain spills) copy of the third
edition (1972) from the wreckage of an old histology lab about 20 years
ago.

Indeed, Patsy Ruegg! Decal is a trademark of the Decal Chemical
Corporation and should not be used generically for decalcifying
solutions. See decal-bone.com

Bob Richmond
Samurai Pathologist
Knoxville TN

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This e-mail message and any attachments are proprietary and confidential 
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RE: [Histonet] Slide/Block Retention

[Podawiltz, Thomas]

We are state inspected under CLIA, which block retention is two years, however 
we follow the more stricter CAP of ten years.  



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney
Sent: Monday, October 03, 2011 10:23 AM
To: rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu; mjdess...@wvhcs.org
Subject: RE: [Histonet] Slide/Block Retention


CAP standard is 10 years for tissue blocks.  I would caution not keeping them 
for that period for several reasons.  One is that it may put you in a bad 
position regarding legal cases.  You could be asked why you don't follow the 
most strict standard and be expected to explain why.  The second is for 
subsequent patient care.  When Her-2 first come on the scene we were pulling 
blocks from cases older than 2 years old.  You never know what new 
prognostic/predictive markers will appear on the scene.  If you don't have the 
original tumor material you may be doing your patients a disservice.  If I had 
blocks at your institution I would want to keep them myself rather than have 
them destroyed.Jan M.Omaha

 Date: Mon, 3 Oct 2011 07:00:51 -0700
 From: rjbu...@yahoo.com
 To: histonet@lists.utsouthwestern.edu; mjdess...@wvhcs.org
 Subject: Re: [Histonet] Slide/Block Retention
 CC: 
 
 We keep our blocks 9 years.
 René J.
 
 --- On Mon, 10/3/11, Dessoye, Michael J mjdess...@wvhcs.org wrote:
 
 
 From: Dessoye, Michael J mjdess...@wvhcs.org
 Subject: [Histonet] Slide/Block Retention
 To: histonet@lists.utsouthwestern.edu
 Date: Monday, October 3, 2011, 9:50 AM
 
 
 Hello Histonet,
 
 What policy is everyone following for slide and block retention?  We are
 not CAP, however we currently keep slides and blocks for 10 years.  My
 director wants to decrease that period to 2 years, which is the Joint
 Commission standard.  I would like to keep 10 years because we
 frequently are asked to send slides for consult and review that are
 several years old.  It is also helpful when looking for hard-to-find
 control tissue.  However these reasons are apparently not sufficient.  
 
 I'm leaning towards keeping the 10 year policy, but I need additional
 justification.  Or does anyone think the 2 year period is sufficient?
 
 Thanks,
 Mike
 
 Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health
 Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org  |
 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax:
 570-552-1526 
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[Histonet] RE: Microwave ventilation

[Podawiltz, Thomas]

Mine is ducted directly into our ventilation system 


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Duffett, 
Christopher
Sent: Wednesday, September 28, 2011 11:00 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microwave ventilation

Hi All,

I am looking for anyone that is using a vented microwave for special
stains. I need a company to come and inspect the microwave to ensure the
ventilation  system is working correctly. Any help would be great. 

Chris Duffett 
Pathology Manager
St. Joseph's Regional Medical Center
703 Main Street
Paterson, NJ 07503 
Office 973-754-3541
Fax 973-754-3649
duffe...@sjhmc.org



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RE: [Histonet] Xylene sensitivity

[Podawiltz, Thomas]

Sometimes a new employee is like the old canary miners took with them. They 
alert you to bad air. Sometimes, after you have used a product for a while your 
body has adjusted to it and you cease to notice it. 

We use xylene, the last time a substitute was looked at one of the techs had a 
reaction to it so we stayed with xylene. 
The lab is negative pressure, the air exchange rate is 52 times per hour and 
all the equipment that generates fumes is either in a large hood or directly 
vented into the fume system. We also test for xylene and formalin once a year 
which is a OSHA requirement and the main hoods are inspected and tested twice 
per year. 

We have done everything we can to keep our air fresh no matter what chemical or 
reagent we use. 

Bottom line: Not all people can work in a Histology lab plain and simple. If 
someone starts reactions to our reagents as per our guidelines they will be 
sent to Employee Health for review. 

Tom

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Glen Dawson
Sent: Tuesday, September 27, 2011 12:27 PM
To: rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene sensitivity


Rene',
 
I must respectfully ask: what happens after you modify your procedures for this 
new employee  2 days after everything has been standardized/validated another 
employee begins employment, but exhibits sensitivity to the new xylene 
substitute?  I realize that getting another job NOW is not easy, but redoing 
standardized procedures over and over could drive a lab into the ground and ALL 
of the techs in that lab could be searching for another job.
 
It is a mistake to automatically assume that substitutes cannot be toxic or 
cause sensitivity themselves.
 
Glen D.
 
 
 



Date: Tue, 27 Sep 2011 08:44:35 -0700
From: rjbu...@yahoo.com
Subject: RE: [Histonet] Xylene sensitivity
To: sdatt...@stormontvail.org; histonet@lists.utsouthwestern.edu; 
ihcman2...@hotmail.com






Glen:
I respectfully disagree. Do you have any idea what would mean to that employee 
to try to get another job NOW?
Changing the procedure is what we (as histotechnique refers to) have been doing 
for ever.
Perhaps you do not remember other methods, but I do because I started working 
in this field in 1952.
Back then I processed tissues manually and used aniline oil to clear, benzene 
 as ante medium and was very glad when I switched to white gasoline and later 
to xylene.
Do you know why xylene became so prevalent? Because in 1943 the AutioTechnicon 
was developed and they recommended xylene as the way to go.
That selection also was the choice of the VIP manufacturers, and we all started 
to change from the toxic benzene and aniline oil to the toxic xylene.
There are other ways of doing things and changing work or exposing to toxic 
chemicals is not the correct path, no matter how much we are used to.
René J.

--- On Tue, 9/27/11, Glen Dawson ihcman2...@hotmail.com wrote:


From: Glen Dawson ihcman2...@hotmail.com
Subject: RE: [Histonet] Xylene sensitivity
To: sdatt...@stormontvail.org, histonet@lists.utsouthwestern.edu
Date: Tuesday, September 27, 2011, 11:11 AM



Shelley,

Perhaps this employee should look into other job opportunities.  The histo lab 
is filled with chemicals that can cause sensitivity in those people who may be 
prone to that.  Unfortunately, some of the main players in the histo lab 
(xylene  formalin) are often the culprit, but they are the best products in 
terms of how well they work, how universally they are used, how well their 
characteristics are known, etc...  I hate to sound like I'm not sympathetic, 
because I truly am, but I think it is a mistake to modify a histology lab's 
procedures to accomodate a new employee.

Just my opinion,

Glen Dawson  BS, HT(ASCP)  QIHC
Milwaukee, WI


 Date: Tue, 27 Sep 2011 08:27:19 -0500
 From: sdatt...@stormontvail.org
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Xylene sensitivity
 
 Hi all,
 I have a new employee who is developing a scratchy, painful throat and some 
 difficulty breathing when exposed to xylene (for instance, when the cover is 
 raised on the coverslipper). This is her first job in a lab of any sort. We 
 are investigating all the usual culprits--air handling system, hoods, 
 allergies or virus unrelated to histology, etc. Right now she is wearing a 
 PAPR to work, which is obviously not a long-term solution. Ultimately, I 
 think we will conclude that this employee has a sensitivity to xylene and 
 possibly other chemicals in the histology lab, as other employees are not 
 complaining about symptoms related to chemicals.
 
 Does anyone have any experience with activated charcoal air cleaners? I am 
 looking at a portable unit that sits on casters and provides 4 air exchanges 
 per hour. It's not cheap at $1000, but well worth it if it will provide 
 relief for this employee and allow her to continue 

[Histonet] RE: AFIP Closing

[Podawiltz, Thomas]

While I was in the Navy 77-83, I spent a week at AFIP for special stain course 
they had. I got to meet Lee Luna, but did not get to spend a lot of time with 
the man. However, my most memorable trip to AFIP was when I was stationed at 
the National Naval Medical Center in Bethesda, Maryland (the old center). 
I was sent to AFIP with a box of specimens to deliver to one of the labs and my 
duty driver got lost. I had read the manifest of the contents and when the 
driver asked me if I had any idea of where were, I said No and then followed 
up with this statement. You mean to tell me that we have five brains in this 
car and we can't figure out where we are? Yep, I was delivering three brains 
from an air crash accident for study. My duty driver could not wait to get rid 
of the box. 


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220

Tom

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne
Sent: Monday, September 12, 2011 1:14 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] AFIP Closing

 Sad that is where I went thru Histology Training at the Tri-Service School of 
Histotechnology in 1985.  I was in a class of 10 Air Force guys and I was Navy 
and there was one other Navy guy.  I was the only female in my class.  It was 
interesting and have been doing Histology non-stop since then- wow 25+ years 
has gone by fast.  


Helayne Parker, H.T. (ASCP)
Pathology Section Head

Skaggs Regional Medical Center
The Best Place to Get Better

P.O. Box 650, Branson Missouri 65615
Direct: 417-335-7254
Fax: 417-335-7127
E-Mail: hpar...@skaggs.net
Web: www.skaggs.net
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RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin

[Podawiltz, Thomas]

I have had one tech finish an online HT course and currently a second person 
looking at going the online route to her HT. In both cases as their supervisor 
I have had to sign on as their in house trainer. As a trainer it is my job to 
make sure that they know how to function well in Histology when they are 
finished. 

My first tech was working elsewhere when she started school and receive little 
support with her studies, one of the main reasons she came to work for me was 
how appalled I was to hear that she was getting not support. By the time she 
was finished I would of let her work on any specimen removed from either myself 
or anyone in my family. 

We get the HT's that we work to get. As a supervisor and trainer, I am only as 
good as my staff makes me look. If I can go on vacation and the lab does not 
miss a beat, then I have done my job correctly. 

So to everyone that gets a fresh tech who got short changed on their training, 
don't whine about it, take it as an opportunity to shape them to the HT that 
you need them to be. 

Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta
Sent: Wednesday, August 31, 2011 10:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a 
Pin

You might be surprised I too agree with Joyce and Richard. 

I understand very well that a new graduate will not be up to the skill
level of an individual that has been working for a while. What I am
surprised about is that this program seemed to teach to pass the test
and has left all of the technical skills left to be taught by the
persons 1st job. This could lead to several painful experiences for not
only the facility but the new HT.

Were is the disconnect. If a person is doing the OJT route they need to
have at least one year of experience signed-off by a pathologist. If a
person goes through a program who is responsible for making sure that
the base-skills are there? Is there not some standards that a new
graduate should be able to cut/embed so many blocks in an hour? Is it
reasonable for a new graduate to take 1.5 hours to cut 5 (uterus,
appendix, tonsil) one-cuts? From facing to lifting the slide off the
water-bath? Or to take 2hrs to embed 15 (large tissue sections) one-cut
blocks?

So Richard is also right how is a new grad to get experiance without
that 1st gig? But how much resposiblity should be placed on the 1st gig
to train a new HT that is supose to have base skills in lab equipment,
cutting, embeding, staining etal? 

More thoughts
ciao
Matt Lunetta BS HT(ASCP)




Edwards, Richard E. r...@leicester.ac.uk

If you do not employ recently trained individuals, how on earth are they
going to obtain the experience that Matt craves, it's beyond me. My
daughter recently qualified as an Occupational Therapist, when she asked
for the reason that she was unsuccessful in obtaining a post, they said
that as a recently graduated student she had no experience, what
rubbish!!.Thankfully she has now obtained a position under more
enlightened management that is to be found here!.

Cheers

Richard Edwards
 Shirley A. Powell 08/30/11 6:32 PM  
I second that Joyce. 
sp 

 
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
[jwe...@sjha.org] 
Sent: Tuesday, August 30, 2011 6:17 PM 
To: Matthew Lunetta; histonet@lists.utsouthwestern.edu 
Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To
stick a Pin 

I don't understand how a student of any program would have not a portion
of their program dedicated to these skills. We partner with Darton
College and their students to do a certain number of hours for their
Clinicals. They know how to do those things, are trained by the
clinical coordinator for the program, and are graded on their work. 

Are they prepared to go into a lab and work like they've done OJT for
1-2 years? Not at all, but they need to be hired with the understanding
that they will need time and patience to develop their speed and their
skill. 

My 2 cents... 


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew
Lunetta 
Sent: Tuesday, August 30, 2011 13:59 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To
stick a Pin 

Hey all, 

I found Peggy's comments on why the practical was discontinued to be
very interesting. Of late I have had some experience with a new HT that
graduated from a 

RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin

[Podawiltz, Thomas]

Just to let you know, I don't let new techs near prostate, liver or any other 
kind of needle biopsy for at least a year. Why put them under that kind of 
pressure? I want them to stay in the field. I figure working for me is torture 
enough. We give new techs up to three years to get up to speed and pass the 
exam. 


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: Matthew Lunetta [mlune...@luhcares.org]
Sent: Wednesday, August 31, 2011 1:29 PM
To: joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu; Podawiltz, 
Thomas; b-freder...@northwestern.edu; hborg...@wakehealth.edu; rjbu...@yahoo.com
Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a 
Pin

Tom,

I feel that this is very important conversation, not whining about the 
situation but shining a very bright light on an important subject. I agree that 
it is an opportunity to help shape a new HT if several factors are in place, 
the facility can afford the time, cost and personnel that it takes to train.

Oh by and by the new HT is coming along and it will take months (much longer 
than the 3 probationary) to get them to the right place, luckily we can afford 
(just barley) the time, cost and personnel. At this time 2 months into the 
process they are not cutting small BX, STATS, embedding of BX, no immuno work 
and are not to be left alone in the lab. We are hopeful for a good outcome.

Matt Lunetta BS HT (ASCP)





 Podawiltz, Thomas tpodawi...@lrgh.org 08/31/11 8:59 AM 
I have had one tech finish an online HT course and currently a second person 
looking at going the online route to her HT. In both cases as their supervisor 
I have had to sign on as their in house trainer. As a trainer it is my job to 
make sure that they know how to function well in Histology when they are 
finished.

My first tech was working elsewhere when she started school and receive little 
support with her studies, one of the main reasons she came to work for me was 
how appalled I was to hear that she was getting not support. By the time she 
was finished I would of let her work on any specimen removed from either myself 
or anyone in my family.

We get the HT's that we work to get. As a supervisor and trainer, I am only as 
good as my staff makes me look. If I can go on vacation and the lab does not 
miss a beat, then I have done my job correctly.

So to everyone that gets a fresh tech who got short changed on their training, 
don't whine about it, take it as an opportunity to shape them to the HT that 
you need them to be.

Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta
Sent: Wednesday, August 31, 2011 10:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a 
Pin

You might be surprised I too agree with Joyce and Richard.

I understand very well that a new graduate will not be up to the skill
level of an individual that has been working for a while. What I am
surprised about is that this program seemed to teach to pass the test
and has left all of the technical skills left to be taught by the
persons 1st job. This could lead to several painful experiences for not
only the facility but the new HT.

Were is the disconnect. If a person is doing the OJT route they need to
have at least one year of experience signed-off by a pathologist. If a
person goes through a program who is responsible for making sure that
the base-skills are there? Is there not some standards that a new
graduate should be able to cut/embed so many blocks in an hour? Is it
reasonable for a new graduate to take 1.5 hours to cut 5 (uterus,
appendix, tonsil) one-cuts? From facing to lifting the slide off the
water-bath? Or to take 2hrs to embed 15 (large tissue sections) one-cut
blocks?

So Richard is also right how is a new grad to get experiance without
that 1st gig? But how much resposiblity should be placed on the 1st gig
to train a new HT that is supose to have base skills in lab equipment,
cutting, embeding, staining etal?

More thoughts
ciao
Matt Lunetta BS HT(ASCP)




Edwards, Richard E. r...@leicester.ac.uk

If you do not employ recently trained individuals, how on earth are they
going to obtain the experience that Matt craves, it's beyond me. My
daughter recently qualified as an Occupational Therapist, when she asked
for the reason that she was unsuccessful in obtaining a post, they said
that as a recently graduated student she had no experience, what
rubbish!!.Thankfully she has now obtained a position under more
enlightened management that is to be found here!.

Cheers

Richard Edwards
 Shirley A. Powell 08/30/11 6:32 PM 
I second that Joyce

RE: [Histonet] RE: Embedding process improvementand competencyassessment

[Podawiltz, Thomas]

Man did that bring back some memories. Bill and I worked together in the same 
Navy lab and he is correct if a block was embedded wrong you would get it in 
either the back of the head or between the shoulders. They stopped throwing 
them at me the day I throw the block back at the microtomist and hit him in the 
head. We were a tough crew that saw a lot, did a lot, partied together a lot, 
but never lost the fact that we were there for patient care and treated all the 
specimens and bodies as if they came from a love one. When it came time for 
Bill and I to become the trainers we were brutal in how to embed, section and 
all other aspects of histology, I mean we only wanted perfection.


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Thursday, August 25, 2011 1:57 PM
To: Heath, Nancy L.; Jennifer MacDonald
Cc: Histonet Listserv (E-mail); histonet-boun...@lists.utsouthwestern.edu; 
D'Attilio, Shelley
Subject: RE: [Histonet] RE: Embedding process improvementand 
competencyassessment

Just to throw a somewhat funny situation into the mix. I learned my
embedding skills in the Navy. The basic method for learning all tasks
was 1. explain it, 2. demonstrate it, 3. do it. Each microtomist was
responsible for taking a good look at the block before cutting it. If
the embedding was not spot-on, the block was always returned for
reembedding. The method of return was to throw it at the back of my
head. They rarely missed. Very quickly, I got tired of being hit in the
head and my embedding improved. Can't do that kind of stuff now, even in
the military. Who knew that histology used to be a contact sport? We've
gotten so soft!

As to the other situation, as a histology supervisor, I was over
cytology in one of the labs I worked at. I doubt that I was much of a
manager to them as I knew next to nothing about their work. However, I
took the time to learn some aspects, and then just pretty much left them
alone. (It was a mutual respect, they pretty much left me alone too.)
They did a fine job of making me look good. I trusted them, and they
didn't do anything to betray that trust. Because of that, I would simply
sign-off on the occasional request. Evals were pretty easy as I simply
interviewed the pathologists, checked attendance and moved on. That
being said, I wouldn't want to have to do it again. They deserved
better, but we made it work, since it wasn't going to change.

Have a great day!

William (Bill) O'Donnell, HT (ASCP) QIHC 
Senior Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 
Check out my podcast at DeaconCast.Net


SERENITY is not freedom from the storm, but peace amid the storm.

Cultivate it in PRAYER!

 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heath,
Nancy L.
Sent: Thursday, August 25, 2011 11:39 AM
To: Jennifer MacDonald
Cc: Histonet Listserv (E-mail);
histonet-boun...@lists.utsouthwestern.edu; D'Attilio,Shelley
Subject: RE: [Histonet] RE: Embedding process improvementand
competencyassessment

Regardless of wether there were 8 blocks or eighteen blocks taking the
practical taught me to be precise with all of the hands on aspects of
Histology. Shame on the older techs from the practical days of not
keeping on top of their game with embedding. My comment was geared more
towards the newbies coming out of histo schools who can pass the exam
with flying colors but sit them in front of an embedding center or
microtome and they are all thumbs! As far as a manager, I myself would
rather have someone who has experience with histology over seeing my
work. Just once again the lack of respect of having the HT/HTL behind
your name.



From: Jennifer MacDonald [mailto:jmacdon...@mtsac.edu]
Sent: Thursday, August 25, 2011 10:58 AM
To: Heath, Nancy L.
Cc: Histonet Listserv (E-mail);
histonet-boun...@lists.utsouthwestern.edu; D'Attilio, Shelley;
Podawiltz, Thomas
Subject: RE: [Histonet] RE: Embedding process improvement and
competencyassessment



I fail to see the correlation of a non HT person supervising the
Histology lab and the lack of a practical exam for HT/HTL staff.  One of
the issues that Shelley brought up was the staff lost or did not develop
their embedding skills.  Submission of a practical exam is not proof of
highly developed embedding skills.  For the HT exam there were 8 blocks
that were submitted (9 slides).  I know of cases where the blocks were
not even embedded or cut by the applicant. 




Heath, Nancy L. nhe...@lifespan.org 
Sent by: histonet-boun...@lists.utsouthwestern.edu 

08/25/2011 07:11 AM 

To
D'Attilio, Shelley sdatt...@stormontvail.org, Podawiltz, Thomas
tpodawi...@lrgh.org

RE: [Histonet] RE: New CAP question

[Podawiltz, Thomas]

I might be wrong here but I thought that each time that you brought in new 
testing, you had to validate it at that time, then had to keep those records 
for the life of the test, plus a couple of years. 


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Thursday, August 25, 2011 12:46 PM
To: Vickroy, Jim; Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New CAP question

I think it may depend on the inspector.  We had something similar happen
in Cytology during inspection.  They had no validation records for their
Thin Prep processing, which they had been doing for years.  They were
required to validate and provide documentation to CAP.
Laurie Colbert

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy,
Jim
Sent: Thursday, August 25, 2011 9:31 AM
To: Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: New CAP question

I hope you're correct.

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046

-Original Message-
From: Martha Ward [mailto:mw...@wakehealth.edu] 
Sent: Thursday, August 25, 2011 11:30 AM
To: Carol Bryant; Vickroy, Jim; histonet@lists.utsouthwestern.edu
Subject: RE: New CAP question

We too have been performing ER and PR for at least 15 years, participate
in CAP proficiency testing and, when we switched staining platforms a
few years ago, validated the new antibody we switched to.   I have
interpreted the standard as necessary if you are introducing ER/PR in
your lab.  In my opinion you would not have to go back and revalidate
something you did years ago just to have something to show at inspection
time.  We had our CAP inspection this summer and a similar question
pertains to the HER2 assay, which we have also been doing for many
years, and that is what I told our inspector, which seemed to satisfy
them. 


Martha Ward, MT (ASCP) QIHC
Manager, Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104

  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, August 25, 2011 12:10 PM
To: 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: New CAP question

Please respond to all.  I would like the information also. 
Thank you,
Carol 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy,
Jim
Sent: Thursday, August 25, 2011 12:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] New CAP question

One of the new CAP questions is ANP.22976 ER/PgR validation.

If the laboratory performs immunohistochemistry for estrogen receptor
and/or progesterone receptor as a prognostic/predictive marker on breast
carcinoma, the laboratory has documented appropriate validation for the
assays.  In the note it says should include a minimum of 40 cases and
validation should be performed by comparing the laboratory's results
with another assay that has been appropriately validated.

We have been doing ER/PR's for over ten years.  Originally we compared
our ER/PR testing with the old immunology method that used frozen breast
tissue.   We also compared our ER/PR results with another hospital.
Problem is that this has been over ten years and we do not keep quality
control records that long.   Am I missing something?
I know we use the FDA approved protocol from Ventana on our Ventana
Benchmark XT.
Should we do another validation study using Ventana or another hospital
that is using the FDA approved method?   Anybody understand what CAP is
wanting and how to accomplish this?

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



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[Histonet] CSF billing.

[Podawiltz, Thomas]

What is the standard CPT code that everyone uses for CSF specimens

Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.

THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential 
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RE: [Histonet] Formalin down the drain??

[Podawiltz, Thomas]

Not just OSHA, but the EPA prohibits it 
http://www.epa.gov/lawsregs/topics/waste.html#hazwaste 



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, July 25, 2011 4:54 PM
To: mtitf...@aol.com; histonet@lists.utsouthwestern.edu; BillO'Donnell
Subject: RE: [Histonet] Formalin down the drain??

Your rant is interesting but wrong.
OSHA (which is a FEDERAL agency) prohibits dumping ANY type of hazardous 
materials down the drain. 
I was also taken aback by Amy's posting.
No, regardless of what your state law may or may not permit you to dump in the 
drain, you should not put some $avings over the well being of the environment 
and the drinking water of people.
Formaldehyde is toxic and recently officially declared carcinogen.
In the same way that frackting methods to obtain gas from shale has been 
deemed dangerous, equally dumping formaldehyde, xylene and any other chemical 
ought to be the source of concern. This in my rant!
René J.

--- On Mon, 7/25/11, O'Donnell, Bill billodonn...@catholichealth.net wrote:


From: O'Donnell, Bill billodonn...@catholichealth.net
Subject: RE: [Histonet] Formalin down the drain??
To: mtitf...@aol.com, histonet@lists.utsouthwestern.edu
Date: Monday, July 25, 2011, 4:19 PM


One should not automtically assume that laws are broken here. 

(Rant begins here)

First of all, it is the States that set the limits of what can and
cannot be dumped. All States must meet Federal standards,but States are
free to determine how they do that. (It's one of the benefits of the
American Revolution) Some states are more heavily regulated than others.
California and Colorado come to mind immediately.

Different organizations, locations and circumstances may allow for
disposal of products that may be diluted to such a degree as to be
negligable in the waste stream. Our institution generates 65,000 gallons
of waste water daily, which allows us to make the dilution limits of
anything that our histo lab could produce in a day. 

No laws are broken if I should pour xylene, formalin, alcohols or other
common compounds that we might generate on even our busiest days into
the waste stream. 

HOWEVER, while we may be allowed to do so by state and local
regulations, we have decided it is not prudent to do so and so we
collect, ship, neutralize or recycle most all that the histo lab
generates. We do this at the lab level, with lab funding. It is the
responsible thing to do, and we are morally and ethically bound to do
so, but we are not outside the law if we do not.

If your local municipal waste systems people give you the green light on
dumping formalin down the drain. you are not breaking the law,
federal or otherwise, in doing so. 

It is true that if you wish to affect things globally, one has to be
responsible locally.

Here is what my rant comes down to Make certain that you are meeting
local standards for your chemical disposal or you may well be breaking
the law. And a big thank you (from myself, my children, grandchildren
and great-grand children and that lady who sells me the slurpee at the
local convenience store) for anything anyone is doing above and beyond
that. 

:)Rant is over... Have a nice day :)  

You cannot Like this rant on Facebook or follow this rant on Twitter. 

Bill


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
mtitf...@aol.com
Sent: Monday, July 25, 2011 12:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin down the drain??



I was a little distressed to read the message from Amy in Camp Hill,
Pennsylvania declaring she dumps everything (and I mean everything)
from her histology lab down the drain. There are a bunch of Federal Laws
governing handling and disposal of chemicals used in the histology
laboratory and she appears to be breaking several. The wastewater law
limits how much formalin you can discard down the sink (and you cannot
dilute as you go). The same law forbids disposal of organic solvents
like xylene, or solutions containing organic solvents. Local laws in
Pennsylvania may be more strict.

I recommend to Amy that she purchases a book like, Hazardous materials
in the histopathology laboratory by Janet  Richard Dapson and read the
whole thing cover to cover!

Michael Titford
Pathology USA
Mobile AL USA

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RE: [Histonet] Paraffin Wax Waste Disposal

[Podawiltz, Thomas]

We dispose of our waste paraffin as a chemical waste. It gets hauled away, 
since we have no way to incinerate it.



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa 
[rjbu...@yahoo.com]
Sent: Saturday, July 23, 2011 10:46 AM
To: histonet@lists.utsouthwestern.edu; Joanne Clark
Subject: Re: [Histonet] Paraffin Wax Waste Disposal

I always incinerated my used paraffin
René J

--- On Fri, 7/22/11, Joanne Clark jcl...@pcnm.com wrote:


From: Joanne Clark jcl...@pcnm.com
Subject: [Histonet] Paraffin Wax Waste Disposal
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Friday, July 22, 2011, 4:22 PM


Hi All, we had our CAP inspection yesterday and were cited for disposing of our 
waste wax from the processors in regular waste.  In all my 20+ years of working 
in histology I have never disposed of the dirty wax in biohazard waste.  
Especially now with the newer processors that have very little carry over.  I 
know this is probably state regulated by is anyone aware of a regulation or 
documentation that states what the amount of hazardous chemical in a substance 
must be before it is considered hazardous?  And if so, does anyone know of a 
way to measure the amount of xylene in waste paraffin?

Thanks in advance.
Joanne Clark, HT
Histology Supervisor
PCNM
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[Histonet] RE: Assisting with Autopsies

[Podawiltz, Thomas]

We have always helped with autopsies, just comes with the territory. 



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
susan.wal...@hcahealthcare.com
Sent: Wednesday, July 20, 2011 3:32 AM
To: as...@georgetownhospitalsystem.org; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Assisting with Autopsies 

We are a small hospital and though we no longer do in house autopsies we still 
had an autopsy assistant.(most prefer to be called this) We always had a pool 
of people available to do this job. Training as a histotech does not include 
this job and I have always refused to do it. I know there are techs who do not 
mind and some who supplement their income doing it but histotechs should not be 
forced to do them, certainly not free of charge. If enough histotechs in an 
area stand together and refuse the pathologists will find assistants.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self
Sent: Tuesday, July 19, 2011 3:12 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Assisting with Autopsies 

Hello All,

We are small hospital that does approximately 5-10 autopsies a year.  This 
being said our administration department does not want to hire a diener to 
assist with these autopsies. So I have decided to turn to all of you out there 
in histoland for a little poll.

Does your facility use histotechs or a diener to assist with the autopsy?


Thanks in advance for all of your help, Amy


Amy Self
Georgetown Hospital System
843-527-7179
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RE: [Histonet] Retirement

[Podawiltz, Thomas]

And that is why I never let you use my knives. 

Tom

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Monday, June 20, 2011 10:31 AM
To: histot...@imagesbyhopper.com; Paula Sicurello
Cc: histonet@lists.utsouthwestern.edu; Amos Brooks
Subject: RE: [Histonet] Retirement

You had COTTON in your pipettes? We used hollowed out reeds with a bit
of papyrus in one end. No calibration But plenty accurate enough for
histo in those days.

However, there are a few things I do not miss from back in da' day.
Hand-stropping a knife for an hour, only to have it nicked by a staple
in the next hour. 

-Bill

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histot...@imagesbyhopper.com
Sent: Sunday, June 19, 2011 7:48 PM
To: Paula Sicurello
Cc: histonet@lists.utsouthwestern.edu; Amos Brooks
Subject: Re: [Histonet] Retirement

And remember mouth pipeting? oops, that's the cotton...

Eating and smoking in the lab was the norm.

Our alcohol had the tax stamp on it!  :o)

Michelle


On Jun 19, 2011, at 4:19 PM, Paula Sicurello pat...@gmail.com wrote:

 Sheesh!  We used to have people smoke while working with propylene
oxide.
 
 Eating in your control pigs was part of the benefit of being a 
 graduate student to save on grocery money.
 
 Film?  My TEM used glass plates.
 
 Lab mates used to routinely drink diet coke and 100% ethanol on
Fridays.
 
 Wearing closed toed shoes was for wimps, you were just fast if you 
 dropped a steel wedge blade.
 
 We even wrote using the entire word and proper grammar, none of this 
 acronym stuff for us.
 
 Retirement?  What's that?
 
 Paula :-)
 
 On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks amosbro...@gmail.com
wrote:
 Agarose Gels!
... Listen you whipersnapper Agarose is the easy way out. When I 
 learned it we used to have to make up our own polyacrylamide gels. 
 That was after having to walk to work up hill both ways in 30 feet of
snow!
 
 (No nearer retirement)
 Crotchety Amos
 
 
 
 Message: 7
 Date: Fri, 17 Jun 2011 13:24:12 -0400
 From: Emily Sours talulahg...@gmail.com
 Subject: Re: [Histonet] Retirement
 To: histonet@lists.utsouthwestern.edu
 Message-ID: banlktinejxtxyop-byfweuxn3yw-ff3...@mail.gmail.com
 Content-Type: text/plain; charset=UTF-8
 
 Retirement? I think by the time I get to that point, social security 
 will have run out.
 Then again, technology will be so advanced, I can tell stories about 
 the old days, where I logged on to the bbs by modem to post messages 
 to my friends and typed in my own html coding.
 We didn't have google when I was young!! Our cameras used film! And 
 you couldn't see how bad your pictures were until you developed that
film!!
 There was no PCR to sequence your DNA, you ran an agarose gel and 
 hoped for the best!! You could drink the 100% ethanol, there was no 
 denaturing! (okay that was before my time) You could smoke in the lab

 while you sectioned without gloves!! (okay that was too)
 
 Emily
 
 A great book should leave you with many experiences, and slightly
exhausted.
 You should live several lives while reading it.
 -William Styron
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RE: [Histonet] Retirement

[Podawiltz, Thomas]

I 'm game for that as long as there is Tequila around. 



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
 ___
From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson 
[robin...@mercyhealth.com]
Sent: Friday, June 17, 2011 12:13 PM
To: Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retirement

Bill,

I am about the same age as you are and would like the same type of locale. 
Since there are so many of us in this field due to retire in the next 15-18 
years maybe we should consider investing in a Histotech Retirement Community. 
Specimens could be shipped to us for processing and staining. We could set up 
microtome stations in the common area and we could get together and talk and 
cut at the same time and do it in a wonderful climate with nice views and of 
courseat our own pace which will be relaxed and more of a shuffle that a 
full out sprint. Anyone have any suggestions for naming such a paradise?

Ok...I'm just getting old and it is Friday..so hope you appreciate my humor.

Have a good weekend everyone!
From here in the Midwest, where waterfront property is in abundance along the 
Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


 O'Donnell, Bill billodonn...@catholichealth.net 6/17/2011 11:00 AM 

 OK, I know it is Friday, and I know that this may sound like a bit of a
jokebut I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC  PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at b...@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC
Senior Histologist/Safety Officer
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.






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RE: [Histonet] Retirement

[Podawiltz, Thomas]

For our own commune, I would look at Bend, Oregon, nothing against northern CA  
it is so beautiful, buts its still CA. 
Plus the fact, I was born and raised in Oregon. 

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
 
From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garcia, Lori, Sr. 
Scientist [lori.gar...@medtronic.com]
Sent: Friday, June 17, 2011 4:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

 Count me in too! Somebody will have to be around for when the robots shut down 
or revolt and try to take over the world.

I'm a midwesterner like Cindi, but have relocated to northern CA and it is 
paradise out here. We could get some land out in the boonies and start our own 
histo commune.

Lori

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, June 17, 2011 11:33 AM
To: Cynthia Pyse; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement


 I have recently traveled back from the future, and it does not bode well for 
histologists. The robots are pretty reliable and can operate 14 hours on a 
single charge. They also do not have to wear safety glasses when working with 
the laser microtomes. No vacations, no calling in sick, no travelling to 
seminars (new software version upgrades come to
them)

I noticed that someone still has to come in in the morning to flip the 
switches, but since CLIA will not likely consider that testing Well, who'll 
need a histologist for that?

Funny though, pathologists are still around, but they are all workiing in a 
single building near Area 72. (you don't want to know about area 72 unless you 
are still around in 2035, then EVERYONE will know about area
72)

They spend their full 6 hour day looking at scanned images on big-screen 
monitors and drinking coffee. It's not that the future really needs them, but 
the AMA still lobbies for them more effectively than the ASCP for us.

- Bill

With apologies to all the really decent pathologists who monitor this forum. (I 
have to be nice because my only usable skill someday might be making a mean cup 
of coffee)

-Original Message-
From: Cynthia Pyse [mailto:cp...@x-celllab.com]
Sent: Friday, June 17, 2011 12:51 PM
To: O'Donnell, Bill; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

As long as we don't have to go back to steel knives, old AO micrtomes and the 
autotechnicon I'm in. I am also 15 to 18 years out. What is the world of 
histology going to do without us. Who will know how to make a solution of 
mucicarmine (not that I do anymore, but I could) or eosin?
The tech I train now look at me like I am speaking a foreign language 
sometimes. Make me feel old, but closer to retirement.
Cindy

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, June 17, 2011 12:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement


 OK, I know it is Friday, and I know that this may sound like a bit of a 
jokebut I am 15-18 years out from retirement and my wife and I want to 
retire someplace tropical And it would be smart to get settled in such a 
location. So, if anyone knows of any openings in Hawaii, Virgin Islands, St. 
Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC  PLEASE let me know. Would be open to others, but would prefer 
a US territory. I can be reached at b...@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety Officer Good 
Samaritan Hospital 10 East 31st Street Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.






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[Histonet] Unlabeled specimens

[Podawiltz, Thomas]

What are your procedures for rejection and correction when you receive either a 
surgical or Nongyn specimen that  has incomplete information on the 
requisitions or the container is not labeled properly?
Do you send it back to the provider's office?
Have them come to your lab to make the corrections?
Throw away the specimen?

Tom

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RE: [Histonet] Histology Waste in Florida

[Podawiltz, Thomas]

Thought it was a EPA offense to pour flammables down the drain, especially 
anything that contains methanol. 


Tom Podawiltz HT (ASCP) 
Histology Section Head/Laboratory Safety Officer


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Saturday, April 30, 2011 10:41 AM
To: histonet@lists.utsouthwestern.edu; histot...@imagesbyhopper.com
Subject: Re: [Histonet] Histology Waste in Florida

Formalin → towed away by a safety contractor.
Alcohol → down the drain (recycling alcohol is too costly and time consuming).
René J.

--- On Fri, 4/29/11, histot...@imagesbyhopper.com 
histot...@imagesbyhopper.com wrote:


From: histot...@imagesbyhopper.com histot...@imagesbyhopper.com
Subject: [Histonet] Histology Waste in Florida
To: histonet@lists.utsouthwestern.edu
Date: Friday, April 29, 2011, 6:04 PM


Hi Histonetters,



This is a question specific to Florida.  Can you share with me what your
facilities do with regards to the disposal of formalin and alcohol?  With
regards to formalin, do you recycle, haul it away, neutralize etc?  With
regards to alcohol, do you haul away, pour down the drain, recycle etc?
Does anyone have any specific regs that could help answer these questions?



Thanks!

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RE: [Histonet] IMEB

[Podawiltz, Thomas]

Since my Navy time I have had two major careers in my life time. Histology and 
Sales(17 yrs). 
My experience gained me this prospective: All companies go through periods were 
everything they do is done right, then a few key people leave and suddenly they 
can't get out of their own way. Sometimes they figure it out and get back on 
track and sometimes they don't and fail. I am not familiar with IMEB at all and 
cannot comment on their status. I will comment on what I always told my staff. 
It is unforgivable not to call a customer back, no matter how bad the news 
is. Never lie, that way you don't have to remember what you said the last 
time you talked to them. Remember this, a bad experience of any kind with us 
travels faster that a good one. Above all never ever lie to me. We had 
customers that loved us because we did what we said were going to do and others 
that hated us because sometimes we just could not get out of our own way. 

By the way, I left my last sales position because of the President of Sales 
(minor owner), would not call back customers and would downright lie to them. 
He was later bought out and terminated. 
Thus my return to Histology. 
I think the only point I am trying to make is that there is always two sides to 
the story. 

Tom Podawiltz HT (ASCP) 
Histology Section Head/Laboratory Safety Officer



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, April 28, 2011 10:52 AM
To: Sheila Haas; histonet@lists.utsouthwestern.edu; Kaye Ryan
Subject: RE: [Histonet] IMEB

Kaye:
In no way this posting is aimed at roasting yours, not at all, it is just to 
explain MY philosophy when participating in HistoNet.
When I read a posting first I consider if I have something useful to contribute 
and if that is the case I post my answer and I really do not care if it will 
follow a consensus, it is my answer based on my experience and from the start 
I consider that I may be wrong or that others' answers will reflect reality in 
a better way.
The fact that you may have a good commercial experience with IMEB in absolutely 
no way excludes the possibility that others' experiences could have been 
disastrous.
Additionally, it does not really matter how others have related to this company 
if the one posting the initial query has had a bad experience.
Summing up do as I do: post your answer if you consider will help, and 
absolutely disregard what others may think of it, and never try to impose your 
criterion.
René J.

--- On Thu, 4/28/11, Kaye Ryan kr...@nfderm.com wrote:


From: Kaye Ryan kr...@nfderm.com
Subject: RE: [Histonet] IMEB
To: Sheila Haas micropathl...@yahoo.com, histonet@lists.utsouthwestern.edu
Date: Thursday, April 28, 2011, 10:32 AM


I usually do not respond to postings because I have seen people that may not 
agree with a posting get roasted by others but I felt like I had to chance it 
with this posting.  I have had many excellent transactions with IMEB and would 
recommend them to anyone.  Their products are trustworthy and their prices are 
excellent compared to other companies that sell the same products.  I have 
known the owner and several of the reps for many years and have never heard of 
an incident like this.  I too, had to put a deposit down when I made my first 
order because we did not have an established account with them.  I am assuming 
that you did not have an account already established since they were checking 
your credit status?  It does take time to fill out the paper work and for them 
to check your credit references.  It is that way with almost any new company 
that you place a large order with.  In this day and time trust is a hard 
thing to come by on a new
 account.  It is terrible that people that may not have used them now have a 
negative thought in their minds about the company. We all have at some time 
something that we can find not quite to our liking about any company and I 
think we need to be careful when we are saying statements like  It appears 
there have only been a couple of successful recent transactions with them.  
This can be interpreted by some to mean that there have been many negative 
interactions so stay clear of them.  Maybe a lot of their customers do not 
reply to posting or do not even subscribe to the histonet.

Kaye Ryan
Histology Manager




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Thursday, April 28, 2011 7:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IMEB

Thanks to everyone for the feedback on IMEB. It appears there have only been a 
couple of successful recent transactions with them. Most of the feedback I 
received was not favorable so it appears the company is in
a good bit of disarray for reasons unknown. At this point, I've not gotten
a response from the owner 

RE: [Histonet] Competancies for handling hazardous material

[Podawiltz, Thomas]

We did a self audit about three or four years ago. My area is considered a 
small waste storage area and yes there is required training. In New Hampshire, 
we can get that training through the state. 




Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fortin, Joyce 
[joyce.for...@uhsinc.com]
Sent: Wednesday, March 02, 2011 1:21 PM
To: Liz Chlipala; Rae Staskiewicz; Histonet
Subject: RE: [Histonet] Competancies for handling hazardous material

Could I PLEASE have this information, too?  I would really appreciate it.


Joyce Fortin
Histology Supervisor
Palmdale Regional Medical Center
38600 Medical Center Drive
Palmdale, California  93551
Phone 661-382-5723
Fax   661-382-5747
email:  joyce.for...@uhsinc.com

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala 
[l...@premierlab.com]
Sent: Wednesday, February 23, 2011 6:51 PM
To: Rae Staskiewicz; Histonet
Subject: RE: [Histonet] Competancies for handling hazardous material

Rae

We have, you need standard training, but the individuals who inspected our lab 
did not mention compentances.  But our inspection happened because we needed to 
switch from a conditionally exempt small quantity generator to a small 
quantitiy generator and we then needed to register with the state, etc.  They 
primarily focused on our waste streams and what chemicals were in the lab.  I'm 
not at work right now, but I'll check tomorrow to see if I have anything that I 
can share with you.

Liz



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rae Staskiewicz
Sent: Wed 2/23/2011 6:19 PM
To: Histonet
Subject: [Histonet] Competancies for handling hazardous material





Has anyone been inspected by the EPA regarding removal of hazardous
chemicals?  It came up in a safety meeting that I should have training and
competencies (beyond general safety training and PPE) for pouring xylene
from staining dishes and processors into accumulation containers.



Rae Ann Staskiewicz

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RE: [Histonet] How many tissues an histotech is suppose to cut per hour?

[Podawiltz, Thomas]

Worry more about quality than speed at this time. I measure my staff on quality 
rather than how fast things get done. It's a waste of time, energy and 
resources to have to re-cut blocks because of poor quality. Speed comes with 
time and experience. 



Tom Podawiltz HT (ASCP) 
Histology Section Head/Laboratory Safety Officer
LRGHealthcare 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole
Sent: Tuesday, March 01, 2011 9:14 AM
To: histonet@lists.utsouthwestern.edu; 'Jenny Vega'
Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per 
hour?

I agree with Cynthia.  Practice and setting up a standard system of how you 
cut.  I do the same thing every time with each block I cut.  Cutting faster is 
not necessarily a good thing if you are jeopardizing quality just to cut more 
slides.  It's quality over quantity in my book any day! 

Nicole Anne Konop BS, HTL(ASCP)
Histology Team Lead
Children's Hospital of Wisconsin
(414)266-6580 Direct Line
(414)907-0366 Pager
(414)266-2524 Histology Department


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse
Sent: Tuesday, March 01, 2011 7:51 AM
To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per 
hour?

Practice, practice, practice!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega
Sent: Monday, February 28, 2011 5:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] How many tissues an histotech is suppose to cut per
hour?

I would like to know how many tissues does a experienced histotech is
suppose to cut per hour (routine he slides) in the microtome. I am a new
histotech and I would like to know how much tissue do an experienced
histotech cuts per hour. For example if you have 100 tissues per total in
how many hours are you suppose to cut this tissue? or.tell me how much
you cut at your lab.

Do you have any suggestions on how to cut faster? Thank You.

Thanks
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RE: [Histonet] CPT code 88363

[Podawiltz, Thomas]

It is my understanding that it used for examaination and selection of retrieved 
archival (ie,previously diagnosed) tissue for molecular analysis (eg, KRAS 
mutational analysis). 
page 440 of the 2011 AMA CPT , professional edition. 



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun 
[rcar...@harthosp.org]
Sent: Wednesday, February 09, 2011 1:12 PM
To: Histonet
Subject: [Histonet] CPT code 88363

Is anyone using the above CPT code for reviewing old pathology slides prior 
to ordering IHC testing or can it only be used for slide review prior to 
ordering molecular testing?  Thanks.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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RE: [Histonet] mislabeling of slides and blocks

[Podawiltz, Thomas]

So who gets fired the person that made the error or the person who let the 
slide out of the lab after verifying that the block matched the number and 
tissue on the slide? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A
Sent: Friday, October 08, 2010 12:41 PM
To: Amy Farnan; histonet@lists.utsouthwestern.edu
Cc: Dean, Sherry
Subject: RE: [Histonet] mislabeling of slides and blocks

I'd be happy to share mine but it is very strict.  People are given a final 
written warning for the first mislabel not caught in the department and can be 
fired for the second within a year.
I highly suggest the Ventana Vantage (there are others but in my opinion 
Vantage is the best) bar code system for patient safety.  It also relieves the 
stress on your Histo techs because the bar coding helps them assure they have 
the correct slide, block, etc.  Techs are hard to find and if we start firing 
for inevitable human error we will cause even more tech shortages.
When you look at all the costs that a mislabel can incur, (law suits, replacing 
personnel, rework, etc.) the cost of the software is not too bad.
Janice Mahoney HT(ASCP)
Histology/Cytology Coordinator
Alegent Health Laboratory
4955 F Street
Omaha, NE
(402)717-2889
fax(402)717-5231

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Farnan 
[farn...@nehealth.com]
Sent: Friday, October 08, 2010 7:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] mislabeling of slides and blocks

Hello everyone,

I was wondering if you could share your policy for employee infractions of 
mislabeling blocks and slides? I would like to get an idea of what the standard 
is out there.  Any help would be appreciated.

Thank you

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RE: [Histonet] Quality Assurance for Histology

[Podawiltz, Thomas]

I actually randomly review the slides before they are sent to the Pathologist 
any slide with incomplete sections, chatter or other major defects get re-cut 
at that time. Since doing this complaints from the Pathologist disappeared 
about the quality of the slides they were getting. They get the QA form with 
the last book of slides for the day. They fill it out then give it back to me. 
Works well for us. I do know this will not work for others, but it works for 
us. 

Tom 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, October 11, 2010 11:21 AM
To: histonet@lists.utsouthwestern.edu; Laurie Colbert
Subject: Re: [Histonet] Quality Assurance for Histology

After many different forms and many efforts to make the pathologists to provide 
feed back about the quality of the sections and procedures, this is what I 
finished doing:
to ask the pathologists to simply separate the slides they considered of poor 
quality and those unacceptable for diagnoses.
It was then my job to define the problem and to addressed it with the histotech 
who made the slide, to determine the re-training or any other administrative 
action deemed necessary.
After I did that I started to receive slides while before seldom any 
pathologist was willing to use any time to evaluate the slides. In all reality 
they are quite busy to take time to fill forms that, in any event, I also had 
to review, re-evaluate and discuss with the histotech.
This procedure worked very well for me and the quality of the work was improved 
considerably, as well as the rejections diminished.
Try this approach.
René J.

--- On Mon, 10/11/10, Laurie Colbert laurie.colb...@huntingtonhospital.com 
wrote:


From: Laurie Colbert laurie.colb...@huntingtonhospital.com
Subject: [Histonet] Quality Assurance for Histology
To: histonet@lists.utsouthwestern.edu
Date: Monday, October 11, 2010, 11:08 AM


I am revising our daily QA sheet that we hand out to the pathologists
with the HE's in the morning. I would like to gather some ideas from
other sites.  Does anyone have a form/chart that they would be willing
to share with me?



Laurie Colbert

Huntington Hospital

Pasadena CA

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RE: [Histonet] FORMALIN DISPOSAL

[Podawiltz, Thomas]

Our gross room is set up a with a fume hood that runs the entire length of one 
wall, this includes our sink. When we need to dump tissue, we plug the drain 
then lay down our formalin pads to collect any spills. We place a large 
specimen container on the pads add a large screen then pour off the formalin 
into the container. We use another container to collect the various parts and 
when full we cap it then place in the waste box that gets incinerated. 
The waste formalin is then de-formalized (Surgipath product), aldehyde and ph 
are check and if they pass we can then drain dispose. If not, we collect it as 
waste which is then stored in drums to be hauled away. This procedure was 
approved by our state DES. 

If you cannot dump the tissue under a hood like this, you will need to wear a 
chemical respirator, all of my staff have their own full face respirators and 
have been fit tested for them. 


Tom Podawiltz HT (ASCP) 
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara 
Baldwin/mhhcc.org
Sent: Wednesday, September 22, 2010 11:56 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] FORMALIN DISPOSAL

Histonetters:
Our disposal company for formalin wants us to separate our stored tissues from 
the formalin and then pour the formalin into a drum.
Does anyone have a procedure that doesn't expose your histotechs when pouring 
formalin off stored tissues, the company states there isn't a container that we 
can use to put the formalin and tissues in without being non-complient.  Does 
anyone have a suggestion?

Thanks
Pathology Supervisor
Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbald...@mhhcc.org
Ph 812-482-0210, 482-0216,  Fax 812-482-0232, 
Pager 812-481-0897
Confidential information, Authorized use only.
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RE: [Histonet] new lab design

[Podawiltz, Thomas]

FYI, 

I have been to Steve's lab. They have a great layout. A lot of time and effort 
was spent in the design of it and it shows. 


Tom Podawiltz HT (ASCP) 
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen
Sent: Monday, September 13, 2010 12:29 PM
To: Blazek, Linda; Histonet
Subject: RE: [Histonet] new lab design

Hi Linda,

We designed one from scratch without having a previous Path Lab in the
hospital before.  We are doing a workshop to that end at NSH in Seattle
(WS 50).  If you cannot attend the workshop, I will be happy to help in
any that I can. 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Monday, September 13, 2010 8:10 AM
To: 'Histonet'
Subject: [Histonet] new lab design

Good morning all.  I am in the process of designing a new lab.  We have
grown beyond our walls and will be moving to a new building.  If anyone
has any great suggestions or ideas they would like to share I'd love
your input!
I'm still looking for a couple of tech too!
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services Linda Blazek HT
(ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists,
Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email:
lbla...@digestivespecialists.commailto:lbla...@digestivespecialists.com


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RE: [Histonet] Acceptable way to bake sections onto slides?

[Podawiltz, Thomas]

Totally agree with Rene. You will need to use some type of drying oven 


Tom Podawiltz HT (ASCP) 
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, August 13, 2010 8:36 AM
To: histonet@lists.utsouthwestern.edu; PhilipGibson
Subject: Re: [Histonet] Acceptable way to bake sections onto slides?

The correct answer is b: if there is water underneath the section that is 
going to be placed on the hot plate, the most likely artifact is that of empty 
nuclei that will ruin the sections' usefulness.
René J.

--- On Fri, 8/13/10, Gibson, Philip philip.gib...@nuth.nhs.uk wrote:


From: Gibson, Philip philip.gib...@nuth.nhs.uk
Subject: [Histonet] Acceptable way to bake sections onto slides?
To: histonet@lists.utsouthwestern.edu
Date: Friday, August 13, 2010, 8:23 AM


Hi



In our fairly large histopathology lab we're hoping to consolidate our
autostainers and coverslippers to only two (Sakura) machines.

In order to efficiently do this, we would need to bypass the
autostainer's oven so that multiple racks of slides can be processed
continuously without any 10-15 minute hold-ups. Therefore, we would like
our four separate microtomist teams to place freshly-cut sections on a
hotplate to bake for 10 minutes, before being picked up into racks and
transferred directly to xylene on the autostainer.



My question:



Do hotplates work well enough to do this? Two conflicting views in my
lab are (a) Yes, this would work in my experience, and (b) No, this
creates artifacts because water trapped underneath the sections boils
and does damage.



Of course, the more conventional approach would be to use ovens, but
loading and unloading an oven before the autostainer is an additional
wasteful step.



What do my fellow histonetters think?



Many Thanks



Phil







Phil Gibson

Senior Biomedical Scientist

Histopathology Dept

Royal Victoria Infirmary

Newcastle Upon Tyne

NE1 4LP



Ext. 24565

Tel. 0191 2824565





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RE: [Histonet] Special Stain Storage

[Podawiltz, Thomas]

That is a new one on me. It has never come up during any of our inspections. 

 
Tom Podawiltz HT (ASCP) 
Histology Section Head/Laboratory Safety Officer
LRGHealthcare




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson
Sent: Monday, July 26, 2010 12:08 PM
To: histonet
Subject: [Histonet] Special Stain Storage

Hello All,
We were recently inspected by CLIA and our inspector noticed that we didn't 
have reagent storage temperatures written in our Special Stains procedures.  We 
do our stains by hand so we do have some stored in the refrigerator and others 
stored at room temp.  I went through some of the Histo books and I cannot find 
any specifics on storage of reagents.  Any suggestions??
 
Thanks!!
Kristen


  
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RE: [Histonet] (no subject)

[Podawiltz, Thomas]

That is a hard question. Here when we hire a un registered tech, they are given 
a time limit (3 years) to get their certification. At the end of three years, 
we reserve the right to terminate if they have not achieved their certification.

What are the levels for your lab? Do you have anything like Lab Scientist 1, 2 
or 3 and if so are there pay ranges between each level? 

If there are levels like that, I as manager,  would keep him in level 1 with no 
salary increase until the degree is finished. 


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
  
From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fredrickson, Mona 
[mfredrick...@nrh-ok.com]
Sent: Wednesday, July 07, 2010 1:03 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hello All in Histoland,
I have a tech who is employed as Histotech eligible, but he was not able to 
pass the HT exam and now is no longer eligible to thake the test because he has 
to get his associates degree in science.  The lab management wants to change 
his title from histotech eligible to histotech non-registered without pay 
increase.  But I feel the pay should be increased. So I would appreciate 
comments on the following:
1.) should title be changed from eligible to non-registered?
2.) After having done this for 8 years should his pay stay the same?
3. Should job responsibilities remain the same
 Thank you in advance for feedback!
Histotech in Oklahoma

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RE: [Histonet] Specimen Delivery

[Podawiltz, Thomas]

Here, all the surgical specimens are delivered directly to the Histology lab. 
All other specimens are delivered to the Specimen processing area (SPA). This 
way all specimens that need to be shared can be done there.


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
mwh...@mcleodhealth.org [mwh...@mcleodhealth.org]
Sent: Monday, May 10, 2010 2:35 PM
To: histonet@lists.utsouthwestern.edu
Cc: tbai...@mcleodhealth.org
Subject: [Histonet] Specimen Delivery

For those of you who do tissue/cytology  processing in a hospital or
similar facility: Are tissue or fluid specimens brought directly to the
Anatomic Pathology/Cytology area, or are specimens dropped off in a
clinical accessioning area ?  We are debating the pros/cons of having our
specimens dropped at a centralized location  since our Lab is becoming more
spread out and nurses are confused about where to bring stuff.


Melanie S. White, MT(ASCP)
Laboratory Supervisor, Systems/Anatomic Pathology
McLeod Regional Medical Center
(843) 777-2072


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RE: [Histonet] number of slides

[Podawiltz, Thomas]

We basically do the same at Tom's lab, the only exception is we file the 
unstained slides with the stained slides. Especially with the FNA biopsies. We 
have had to pull the unstained slide to send out for IHC's. 


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar 
[tmcne...@lmhealth.org]
Sent: Wednesday, March 24, 2010 6:11 AM
To: Sue; anita dudley
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] number of slides

We do 2 levels on all GIs, 3 on all needle bx, 3 on cervical, and 2 on bone 
marrows.  We also do special stains up front on the bone marrows, H Pyloris, 
prostates, etc.

We tend to cut a ton of extra slides that we just throw away.  I don't like it 
but I don't think there's any way around it.  Probably like most places, we get 
more and more smaller and smaller biopsies so it's better to keep a few extras 
than to try to go back later.



Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, March 23, 2010 6:08 PM
To: anita dudley
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] number of slides

We do three levels on all diagnostic biopsies. Liver and Kidney also get 
upfront special stains, as do gastric biopsies (h pylori)
We did cut down on extra unstained slides since we were discarding most of 
them. As far as stopping levels, my pathologist thinks it goes against standard
of care. As for prostate biopsies, they are so small any more, that we are 
thinking o cutting 4 slides staining 1and 4 for HE and holding 2 and 3 for 
possible IHC. We
are finding that when we have to go back there is minimal tumor for IHC 
demonstration.

Susan T. Paturzo
Thomas Jefferson University Hospital


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[Histonet] RE: Histo Professionals Day

[Podawiltz, Thomas]

I got into Histology while I was in the Navy. I was out of MLT school by about 
three maybe four months, when my wife who was also active duty finally got 
transferred to the same base as I. There were two openings for her, Blood Bank 
and Histology. The Navy did not want to put a Med Tech in lowly Histology and 
we did not want her working for the Officer in charge of Blood Bank. She had 
previously work for him at another base where his management style got him 
removed from the lab. 

So I gave up my position in Hematology and went to Histology, were I met the 
biggest group of clowns ever. That was 1981. Certified 1985.  Took several 
years off and went into sales. Came back to Histology in 2004 and found that my 
skills were still there and more important the love for Histology. 

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara 
[sbree...@nmda.nmsu.edu]
Sent: Wednesday, March 10, 2010 10:21 AM
To: histonet
Subject: [Histonet] Histo Professionals Day

Perhaps what each of us ought to do is write a quick line naming the one
person that got you involved in histology and how they did that.  I'd be
willing to gather them and pass them on to NSH so they could post it at
the Seattle meeting.  If you'd like to do this, send the email to
nmhi...@comcast.net and I'll collect.  Just an idea...



Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576



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RE: [Histonet] Paraffin Accumulation on floor

[Podawiltz, Thomas]

We just bought everyone skates. 

Seriously, we have a scraper that we use, along with the various mats. What we 
just did was lay the traction strips on the floor outside of the Histology lab. 
That was the area were most people, myself included  have slipped and fallen. 
Ironically, we found no paraffin on the floor. Which is why the traction strips 
were but on the floor. 

The other observation that we made was that most of the people that slipped 
were wearing dress shoes, not idustrial/nursing style foot wear. 

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine 
(CDC/CCID/NCZVED) [j...@cdc.gov]
Sent: Wednesday, February 24, 2010 1:34 PM
To: Scott, Allison D; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Paraffin Accumulation on floor

We have a long-handled scraper and use it periodically.


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Wednesday, February 24, 2010 1:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin Accumulation on floor

How is everyone dealing with the accumulation and tracking of paraffin
problems.  We have mats on the floor where we cut and embed.  We also
have the mats that you walk on and it takes the paraffin off of the
bottom of your shoes and you peel off a layer a day.  Our medical
director wants us to find a way to keep the floors clear, so that no one
will slip and fall.  I have never seen a histololgy lab that did not
have a paraffin tracking problem.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026

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RE: [Histonet] discarding old dry chemicals with no expiration date

[Podawiltz, Thomas]

What CLIA regulation did they use for reference for that statement?

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd 
[kdboydhi...@yahoo.com]
Sent: Wednesday, September 30, 2009 1:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] discarding old dry chemicals with no expiration date

Our lab recently had our CLIA inspection and the surveyor informed me that we 
need to discard any dry chemicals with an opened date of more than 10 years, 
even though none of the dry chemicals have an expiration date. This was not 
written up as a deficiency, but it was suggested we follow up on this for our 
next re-certification.
 I am sure it is best to keep the chemicals current, but is this what all labs 
are doing?


Kelly D. Boyd, BS, HTL (ASCP)
Lab Manager
Harris Histology Services
2025 Eastgate Dr. Ste. F
Greenville, NC 27858
www.harrishisto.com

Tele (252)-830-6866
(800)-284-0672
Cell (252)-943-9527
Fax  (252)-830-0032








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RE: [Histonet] formalin neutralization

[Podawiltz, Thomas]

What does your state require? New Hampshire requires to test with the test kit 
and if at zero we can drain dispose. The state also requires us to keep our 
test records for five years.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stephanie Weaver
Sent: Tuesday, September 29, 2009 11:25 AM
To: histonet post
Subject: [Histonet] formalin neutralization

For those who are better at chemistry than I am:

I have just purchased some Formalex Green to neutralize our waste 10% formalin 
for safe drain disposal.  I noticed that I can also purchase a test kit that 
will tell me if the aldehydes are safely neutralized.  Instead of that, 
couldn't I just add a drop of Schiff's reagent to the final product, and that 
would indicate if there are any unreacted aldehydes?

Thank you in advance,

Stephanie Weaver
Texas Veterinary Diagnostic Lab


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RE: [Histonet] air quality in the lab

[Podawiltz, Thomas]

Our last inspection our exchange rate tested out at 52/hour. We had increased 
from 26/hour because we could still had fumes in the lab. (CMS inspector could 
smell xylene from our stainer) For the life of me cannot remember what the min. 
rate is .

Tom

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of godsgal...@aol.com 
[godsgal...@aol.com]
Sent: Wednesday, September 16, 2009 10:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] air quality in the lab

Does anyone know what the regulations or requirements  are for the air turnover 
in the lab?



 Thanks.
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RE: [Histonet] Company email

[Podawiltz, Thomas]

Just curious, were you trying to use a company credit card? If not try setting 
up a paypal account that is link to your credit card.

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas 
[micropathl...@yahoo.com]
Sent: Monday, September 14, 2009 12:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Company email

I just ran into an interesting issue that I thought I'd share. I was trying to 
place a credit card order with Beckman Coulter but was denied due to them not 
liking my email address. Mind you, I was willing to pay with a credit card. I 
placed the order on 9/2 and have been fighting with them ever since. 
Ridiculous! Today they were standing by their policy of not accepting my email 
address as a business address so I'm taking my business elsewhere. Just thought 
I'd share this with those of you looking for a company to do business with.

Sheila Haas
Laboratory Supervisor
Micro Path Laboratories



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RE: [Histonet] Company email

[Podawiltz, Thomas]

Talk about being ridiculous. Almost sounds like Navy logic


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: Sheila Haas [micropathl...@yahoo.com]
Sent: Monday, September 14, 2009 1:49 PM
To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Company email

Yes, it was a company card. They indicated payment wasn't the issue they
needed the email address for their records. Go figure!

Sheila Haas
Laboratory Supervisor
Micro Path Laboratories




From: Podawiltz, Thomas tpodawi...@lrgh.org
To: Sheila Haas micropathl...@yahoo.com; histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Sent: Monday, September 14, 2009 1:41:06 PM
Subject: RE: [Histonet] Company email

Just curious, were you trying to use a company credit card? If not try setting 
up a paypal account that is link to your credit card.

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: 
histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu
 
[histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu]
 On Behalf Of Sheila Haas 
[micropathl...@yahoo.commailto:micropathl...@yahoo.com]
Sent: Monday, September 14, 2009 12:34 PM
To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
Subject: [Histonet] Company email

I just ran into an interesting issue that I thought I'd share. I was trying to 
place a credit card order with Beckman Coulter but was denied due to them not 
liking my email address. Mind you, I was willing to pay with a credit card. I 
placed the order on 9/2 and have been fighting with them ever since. 
Ridiculous! Today they were standing by their policy of not accepting my email 
address as a business address so I'm taking my business elsewhere. Just thought 
I'd share this with those of you looking for a company to do business with.

Sheila Haas
Laboratory Supervisor
Micro Path Laboratories



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RE: [Histonet] formalin storage

[Podawiltz, Thomas]

I think the 1 gallon per 100 square feet is OSHA.  What you have to make sure 
not to exceed the amount your cabinet is rated. for. We do not store are 10 % 
in a flammable cabinet.



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hayes, Tina J. 
[tina.ha...@va.gov]
Sent: Friday, September 11, 2009 9:37 AM
To: Jean Warren; Riesen, Rebecca; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] formalin storage

WE do not store our 10 NBF in flammable storage.

However, Could you tell me where you found this direction of storing
only 1 gallon per 100 sq. feet in a flammable cabinet, and 1 gallon
outside?  More specific than NFPA.  I have been unable to find that
anywhere, our safety folks tell us that if they are in a flammable
cabinet the amount per room size doesn't matter.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean
Warren
Sent: Friday, September 11, 2009 9:25 AM
To: Riesen, Rebecca; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] formalin storage

No, it is ridiculous. Safety people tried to argue this with us years
ago.
One of our pathologists told them, How can something that is almost 90%

water be a combustion hazard?


- Original Message -
From: Riesen, Rebecca rebecca.rie...@nchmd.org
To: histonet@lists.utsouthwestern.edu
Sent: Friday, September 11, 2009 9:15 AM
Subject: [Histonet] formalin storage




We have been directed by our Safety Officer to store all formalin (37%
and 10% NBF) in a flammable storage room, cabinet or container.  Yes,
37% Formalin we do store in this manner, but I have never heard of this
requirement for 10%NBF.  I looked on line to many MSDS sheets from
different vendors and found only one that stated such storage
requirements for 10% NBF.  During this search I found all but one
company states that formalin is not flammable.  I brought this to the
Safety Officer.  He agrees that it is not flammable but that it IS
combustible.  Combustible=Flash point of 100F to 200F.  Of the dozen
sites I visited I found the following data concerning the Flash Point of
10% NBF: from NA / 200F / 122F to 185F.  The NFPA (National Fire
Protection Agency) guideline of no more than 1 gallon in a flammable
storage container and 1 gallon outside of a safety cabinet/container per
100 square feet is already quite limiting.  Using this guideline, we
have calculated acceptable volumes of the known flammables (Alcohols and
Xylenes) we can store.  Adding 10% NBF to the equation will have us
traveling to our bulk storage area constantly.  Does anyone out there
store 10%NBF in flammable cans/cabinets?
Riesen, Rebecca
rebecca.rie...@nchmd.org
NCH Healthcare Systems
Direct 239-436-5000 x2188
Fax 239-436-6767


Visit our website at http://www.nchmd.org


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RE: [Histonet] Start Up Lab

[Podawiltz, Thomas]

I went to the Navy's MLT program in 79-80 and we did drug screens manually and 
the chemicals that we used would give us a buzz. Thought it was ironic that I 
was getting high while looking for drugs in somebody else.


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello 
[vapat...@yahoo.com]
Sent: Thursday, July 23, 2009 1:30 PM
To: Lynette Pavelich; HistoNet
Subject: RE: [Histonet] Start Up Lab

In the 80's I worked in a drug screening lab for the Navy and one step in the 
extraction involved fuming HCl (meaning pure HCl).  Several of the techs got 
nose bleeds every time they used the stuff.  I finally pried open a window 
(they were painted shut) and we turned on a fan the size of a propeller to blow 
the fumes out the window.

We used Xylene in a room with no ventilation and found out that high 
concentrations of xylene cause transient euphoria.  No wonder we thought 
every thing was funny during those days.

Safety people, they take all the fun out of research!  ;-)

Paula Sicurello

VA Medical Center San Diego

Veterans Medical Research Foundation (VMRF)

Core Research Imaging Center (CRIC)

3350 La Jolla Village Dr., MC151

San Diego, CA 92161

858-552-8585 x2397



Your images flow through our CRIC.

--- On Thu, 7/23/09, Lynette Pavelich lpave...@hurleymc.com wrote:

From: Lynette Pavelich lpave...@hurleymc.com
Subject: RE: [Histonet] Start Up Lab
To: cb...@lexclin.com, montina.vanme...@pbrc.edu, theci...@yahoo.com
Cc: histonet@lists.utsouthwestern.edu
Date: Thursday, July 23, 2009, 3:53 PM

Ah, yes.  The '70's.  Our lab had 2 windows, both on the same side of
the wall.  The pathologists cut under one of the windows and it had one
of those box fans, pointing out.  In Michigan.  No hoods, an old
duo-autotechnicon and xylene in full use with disposal down the drain.
Every day, I would go home not knowing if I went thru a red light or
not, arriving home , head still cloudy for about 30 minutes until it
cleared.  Ah..those were the days??  Although I miss it, I'm glad I had
to give up my coffee and donut when cutting.  Treats in the drawer are
gone, mouth pipetting is gone (not mine, but yes, the mouth does turn
black from silver nitrate!!)  Progress is good.  But I still like my
'70's music!!!  Bob Seger and old motown!!! ;)

 Montina Van Meter montina.vanme...@pbrc.edu 07/23/09 11:31 AM

I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their personal drawer.  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.




Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: theci...@yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
theci...@yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/
Sent from my Verizon Wireless BlackBerry

-Original Message-
From: Lynette Pavelich lpave...@hurleymc.com

Date: Wed, 22 Jul 2009 18:07:07
To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu;
cing...@uwhealth.org
Subject: RE: [Histonet] Start Up Lab


Gosh.I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh..

 Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM 
(lol some labs have a bench area as well as a desk area where food is
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire
cing...@uwhealth.org wrote:

 In the lab?!? For shame. :)

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy
DuBois
 Sent: Wed 7/22/2009 10:29 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Start Up Lab



 And a coffee pot.

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RE: [Histonet] Re: Eosin in alcohol

[Podawiltz, Thomas]

Are the prostate biopsies, fixed in formalin or Bouins?


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda 
[lbla...@digestivespecialists.com]
Sent: Wednesday, July 01, 2009 1:47 PM
To: 'Jennifer Johnson'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re:  Eosin in alcohol

We use about the same amount or eosin in the first 100% alcohol and also do 
immunos on the biopsies and have no problem.  We do all GI biopsies though not 
prostate.  I'm not sure why the eosin would interfere with the immuno staining 
since it disappears during the hydration and retrieval process of staining 
immunos.





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson
Sent: Wednesday, July 01, 2009 1:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Eosin in alcohol


We have used Eosin in the last 95% alcohol on the tissue processor for several 
years.  I usually add approximately 5 ml to the full jug.  It is a great tool 
to use for embedding.  However, we received a letter from the lab that we send 
our prostate biopsies to saying that it was undesirable because it interfered 
with their immuno staining.  They sent us some cobalt blue to use in the place 
of eosin along with mixing instructions and the whole batch of tissues came out 
such a dark blue.  There is no delineations in the color of the blue and I 
found it to be useless for helping to embed.  I would rather do without 
anything than use cobalt blue.  I guess the point of my rambling is, Eosin is a 
wonderful tool to use unless you are doing immunos on prostate biopsies.

Thanks,

Jennifer Johnson, HTL (ASCP)

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RE: [Histonet] tracking turnaround time of intraoperative consultations

[Podawiltz, Thomas]

This is basically how we have always done. On scheduled frozens we have 15 
minutes for turnaround, unscheduled 30 minutes.


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky 
[becky.garri...@jax.ufl.edu]
Sent: Tuesday, June 23, 2009 5:51 PM
To: Della Speranza, Vinnie; histonet
Subject: RE: [Histonet] tracking turnaround time of intraoperative  
consultations

We have just started tracking from order to sign out for frozen
sections.

(In addition, frozens are tracked from receipt in pathology to sign out
using the CAP guidelines).

The trouble with the electronic order (in our institution) is that the
OR may place the pathology order in hospital computer system early in
the surgery so that the order time that prints on the requisition is
substantially different than the actual collect time.

We have resolved this by having the OR staff write the actual collect
time
on the requisition and initial it.  This collect time is also noted in
the OR documentation notes for the surgery.  When OR forgets to note
collect time manually on the requisition (and they do), I call back and
have them  look up and verify the collect time.

This was started with the cooperation and support of the OR
administration.

For the pathology accession staff, this means they can not use the order
time that crosses the interface to the LIS (lab computer system)  but
must enter the handwritten time as noted on the requisition.

We have set a goal of 40 minutes from frozen order to sign out. This may
be lowered to 30 - 35 minutes depending on how our data looks over
several months.  Our pathology dept. is located on the first floor and
the OR on second floor of same building.

As for noting collect times for multiple specimens, same case: We have
always required the OR to generate a requisition for each container.
The collect time is written on each requisition.  This is no different
than
writing the collect date/time and initials that nursing/phlebotomy does
for each tube of blood drawn hospitalwide.

Would be interested in hearing from others on how this is handled.

Becky Garrison
Pathology supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-24-6237



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della
Speranza, Vinnie
Sent: Tuesday, June 23, 2009 4:02 PM
To: histonet
Subject: [Histonet] tracking turnaround time of intraoperative
consultations

CAP utilizes the term intraoperative consultation to describe the
utilization of frozen (cryo) sections to provide a rapid diagnosis back
to a surgeon in the operating room.

The CAP checklist requires a turnaround time of 20 minutes for single
specimen submitted for intraoperative consultation. My understanding is
that the turnaround time is measured from the time the sample is
received in the laboratory until the time the report is issued to the
surgeon.

Is anyone tracking or measuring turnaround time from the time the
consult is ordered in/by the Operating Room until the time the result
is issued?
If so, would you share how you are able to determine the time the test
was ordered  and to what extent you have elicited the cooperation of
Operating Room personnel.

We receive many complex surgical cases and our intraoperative consults
frequently consist of multiple surgical samples from the same patient
arriving in the lab at the same time. Our head and neck cases, for
example, consist of 6-8 biopsies that are sent to pathology at the same
time. In this example, we have no knowledge of which biopsies was
excised first or last and because the surgeon chooses to allow multiple
samples to accumulate before sending them all off to the lab, it's clear
that the true pre-analytical time will not be the same for each
sample.

If you are tracking turnaround from the time of order to the time of
result reporting, how are you determining what is an acceptable
turnaround time? CAP's standard is the only national standard I am aware
of for frozen section turnaround times.



Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974




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RE: [Histonet] (no subject)

[Podawiltz, Thomas]

We do not throw them out, we file them with the stained slides for future 
reference.

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V 
[hor...@archildrens.org]
Sent: Tuesday, March 31, 2009 10:50 AM
To: debra.or...@uchospitals.edu; corne...@hotmail.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

I agree with Lorraine on this one.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
debra.or...@uchospitals.edu
Sent: Tuesday, March 31, 2009 9:43 AM
To: corne...@hotmail.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

Thank you for your very prompt response. I do agree that we are here for
the patient.

Unfotunately I am under the gun to bring down costs and I thought that
might be one way. Thanks again, and look forward to other responses.



Debi





From: Lorraine Cornett [mailto:corne...@hotmail.com]
Sent: Tuesday, March 31, 2009 9:36 AM
To: Ortiz, Debra [UCH]
Subject: RE: [Histonet] (no subject)



Yes,
Unfortunately(financially) it is current practice at our facility as
well.  As a preemptive measure, I keep a record of the number of all
unstained slides(total number only) thrown out on a monthly basis.  The
only alternative is to not cut the extra's and take a chance on not
getting the proper area on the slide for diagnostic stains.  I think
that patient care is of the utmost importance, and this probably is a
small price to pay in the grand scheme of things.


Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division,
Kingsport, TN 423 224-5793 fax 423 224-5349



 Date: Tue, 31 Mar 2009 09:30:18 -0500
 From: debra.or...@uchospitals.edu
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] (no subject)

 We are currently handling all prostate, lung, heart, and breast
biopsies
 as fine needle biopsies (FNB). We cut 10 slides 3 of which are used
for
 HE's and the rest as unstained plus slides. What has happened is that
 we throw thousands of unused expensive slides a year because they are
 not used. Is there a general standard in practice out there for FNBs?
 Unfortunately, with the economy the way it is; and trying always to
find
 ways to bring down expenses this seems counteractive.



 Thank you

 Debi



 Debra Ann Ortiz

 Chief Medical Technologist

 The University of Chicago Medical Center

 Room E-602-A

 5841 S. Maryland Avenue

 Chicago, Il 60637

 phone: 773.702.5237






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 communication is prohibited. If you have received this e-mail in
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RE: [Histonet] Paraffin Block trimming

[Podawiltz, Thomas]

We have one timmer. I don't use it, I hand trim simply because I am quicker 
with it. The other techs use the timmer to save their hands.


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd 
[kdboydhi...@yahoo.com]
Sent: Thursday, March 19, 2009 10:03 AM
To: histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald; Scott; 
Angela Bitting; rjbu...@yahoo.com
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin Block trimming

My techs use our Para Trimmer from Thermo Scientific Shandon. It will save your 
hands, but it takes a lot more time than the old fashion hand scraping. I 
prefer to hand scrape. I can scrape at least twice as many by hand than with 
the para trimmer.


Kelly D. Boyd, BS, HTL (ASCP)
Lab Manager
Harris Histology Services
2025 Eastgate Dr. Ste. F
Greenville, NC 27858
www.harrishisto.com

Tele (252)-830-6866
Cell  (252)-943-9527
Fax  (252)-830-0032



--- On Thu, 3/19/09, Rene J Buesa rjbu...@yahoo.com wrote:


From: Rene J Buesa rjbu...@yahoo.com
Subject: Re: [Histonet] Paraffin Block trimming
To: histonet-boun...@lists.utsouthwestern.edu, Jennifer MacDonald 
jmacdon...@mtsac.edu, Scott lsc...@sfcn.org, Angela Bitting 
akbitt...@geisinger.edu
Cc: Histonet@lists.utsouthwestern.edu
Date: Thursday, March 19, 2009, 9:25 AM


You will have to time worself as I did to find out that waiting fo the paraffin 
to melt in order to eliminate it takes 2.5 times more time than doing it 
manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 
secks./block
René J.

--- On Thu, 3/19/09, Angela Bitting akbitt...@geisinger.edu wrote:

From: Angela Bitting akbitt...@geisinger.edu
Subject: Re: [Histonet] Paraffin Block trimming
To: histonet-boun...@lists.utsouthwestern.edu, Jennifer MacDonald 
jmacdon...@mtsac.edu, Scott lsc...@sfcn.org
Cc: Histonet@lists.utsouthwestern.edu
Date: Thursday, March 19, 2009, 9:13 AM

I've been told that they are slower than scraping by hand. What's the
consensus?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916

No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!


 Jennifer MacDonald jmacdon...@mtsac.edu 3/19/2009 12:26 AM

We have two in our student lab.  It is safer, quicker, and there are a lot
less paraffin shavings to clean up.
Jennifer MacDonald




Scott lsc...@sfcn.org
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/18/2009 09:24 PM

To
Histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] Paraffin Block triming






Hi,
Does anybody use a paraffin block dewaxer ?  If so does it save any
time, how well does it work?

Thanks,

Scott Hendricksen  HT (ASCP)
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RE: [Histonet] Disposal of Formaldehyde

[Podawiltz, Thomas]

We dump very little down the drain. Formalin has not been drained dump since 
1988. We used to have it hauled away, because the former administration never 
got into re-cycling the formalin.
What we do today, is too de-formalize the formalin, test it and then drain 
dumped if it passes. We buy the de-formalizer from Surgipath. We had to get 
this approved the State and we have to keep our records for 5 years. I keep the 
original and send a copy to the Director of Hazard Waste.


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret  
[msherw...@partners.org]
Sent: Friday, March 13, 2009 11:00 AM
To: Jessica Piche; histonet
Subject: RE: [Histonet] Disposal of Formaldehyde

We are discouraged from putting most any chemical down the drain.  The MWRA
(Massachusetts Water Resources Authority) monitors mercury levels, etc. in
Boston Harbor (as well as waterways throughout the state) and will issue steep
fines if labs are found to be dumping such waste down the drain.  Consequently,
our in-house Safety Office and our outside waste management company (Triumverate
Environmental) monitor our waste.Alcohols, stains, and fixatives are
disposed of as hazardous waste.

Peggy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Piche
Sent: Friday, March 13, 2009 10:11 AM
To: histonet
Subject: [Histonet] Disposal of Formaldehyde


Hi All,
We have a question regarding the disposal of formaldehyde. We were told at our
hospital that a consultant said it was okay to dump formaldehyde down the drain.
I believe they said it was okay to dump 15 gallons or so a day! We are not to
fond of this idea and would like to know what everyone else is doing. How is
everyone disposing of their formaldehyde? We would be especially interested in
what other hospitals in CT are doing.
Thanks,
Jessica Piche-Grocki, HT(ASCP)
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RE: [Histonet] log book

[Podawiltz, Thomas]

The OR delivery all the specimens to us. We do not go to the OR at all for 
specimens. Whom ever brings the OR specimens must lay out all specimens with 
the slips. Any case that is missing a specimen, we reject the case and the OR 
runner takes it back to the people that work that case to figure out what they 
did wrong. If we accept a case, we time/date stamp as we sign the OR book. If 
it is after hours the OR delivers the specimens to the clinical specimen 
processing area and they sign for the specimens, then they take them to the 
Histology lab.


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy Gorham 
[gorh...@verizon.net]
Sent: Sunday, March 01, 2009 8:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] log book

Good Monday Morning,  We had a serious incident Friday with O.R.  My aide went 
down to get the specimens from O.R. about 9am. (which were left overs from the 
night before).  She did not stamp in the specimens before she left.  When I had 
time to stamp them in and record them in the log book I discovered that the 
colon was not there.  Two other specimens from that patient where in the bag 
but no colon.  So I went down to O.R. to see where it was.  Of course no one 
knows what happened to the colon.  The doctors are furious by all means.  Now 
the O.R. thinks the path lab screwed up.  So my questions is how do others log 
in the specimens as they come into the lab.  We have 2 couriers that brings 
specimens when we are not in the lab from other hospitals.  How do you make 
sure that whom ever brings the specimens actually brings the ones they say they 
do?  Do you have a log book that every specimen that is brought into the lab is 
written down by the person who brings it in?  Right now we have a log book but 
it is written in as we are accessing  the specimens.  So the specimens may have 
been there overnight. We are a very small lab and we do almost everything by 
hand including writing in the log book.  Someday we want to be able to scan by 
bar codes but right now we can not do that. Thanks for any help you can give me.
Kathy Gorham, H.T.
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RE: [Histonet] Practical Exam

[Podawiltz, Thomas]

Rene, I can't and won't put words into Bill's mouth, especially since we have 
known each other for almost 30 years, but I think his question was, was there 
really a problem with  people turning in someone else's work as their own. 
Where are the facts that it even happen?


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa 
[rjbu...@yahoo.com]
Sent: Friday, February 20, 2009 3:36 PM
To: Histonet; O'Donnell, Bill
Subject: RE: [Histonet] Practical Exam

Bill:
In my time when you accepted somebody else doing YOUR job and YOU being 
remunerated or accredited for it was called CHEATING and that is a moral 
turpitude issue, and really a problem, not as you say that was not much of a 
problem!.
René J.

--- On Fri, 2/20/09, O'Donnell, Bill billodonn...@catholichealth.net wrote:

From: O'Donnell, Bill billodonn...@catholichealth.net
Subject: RE: [Histonet] Practical Exam
To: Histonet Histonet@lists.utsouthwestern.edu
Date: Friday, February 20, 2009, 11:00 AM

If, indeed, these were the two primary reasons for eliminating the practical
exam, they are weak and lazy reasons. An increase in the fee to apply would
cover costs, and, well, was it really that much of a problem of people doing
other peoples practicals.

I can't imagine it to be out of proportion to what might (I emphasize
might and add but likely did not) have occurred
sporadically in all the years prior.

I won't pass judgement on a single source, but would love to hear from
someone who was a part of the decision process that eliminated this practicum.

However, if it is true, my estimation of the ASCP has grossly deteriorated.

William (Bill) O'Donnell, HT (ASCP) QIHC
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, February 20, 2009 8:01 AM
To: Victor Tobias; Histonet; Rittman, Barry R
Subject: RE: [Histonet] Practical Exam

There were 2 fundamental reasons why ASCP eliminated the practical part of the
examination:
1- they got to the conclusion that there was no way to determine if the person
sending the slides was the one who really made them, and
2- it was getting too costly to send the slides to review or to gather the
reviewers to qualify the sections, so they decided to eliminate the practical
and made the changes we have now (renewal and CEU).
René J.

--- On Thu, 2/19/09, Rittman, Barry R barry.r.ritt...@uth.tmc.edu
wrote:

From: Rittman, Barry R barry.r.ritt...@uth.tmc.edu
Subject: RE: [Histonet] Practical Exam
To: Victor Tobias vic...@pathology.washington.edu,
Histonet Histonet@lists.utsouthwestern.edu
Date: Thursday, February 19, 2009, 6:30 PM

Victor
I cannot believe that you have said this.
Although I did not think that the practical examination was the ultimate test
of skill ,  it did at least provide some uniformity.
With an extension of the logic that you use it is just as easy to allow the
pathologist to certify that the technician is qualified even without a written
examination.
Without a somewhat standardized practical there is no guarantee that the
technician will have any practical knowledge outside their individual
laboratory.
Didactic without adequate practical knowledge is, as far as I am concerned,
useless.
What is really needed is a national standardized written and practical test
that is administered by NSH.
I am not holding my breath that this will happen.
Barry


From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias
[vic...@pathology.washington.edu]
Sent: Thursday, February 19, 2009 5:03 PM
To: Histonet
Subject: [Histonet] Practical Exam

There has been discussion regarding the removal of the practical exam.
To me it has not been removed, but the responsibility has shifted to whomever
signs off on the student. In the case of OJT, the pathologist has verified that
this student can cut and stain. Of course what is acceptable to one pathologist
may not be to another. Do they get tested in the art of troubleshooting. As
far as the schools go, they shouldn't be graduating anyone that can't
cut, stain and troubleshoot.
So I don't really see a problem with the absence of the practical. It is
Friday somewhere.

Victor

--
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be contained
in this message. This information is meant only for the use 

RE: [Histonet] Practical Exam

[Podawiltz, Thomas]

Based on what you just said, the students that come from your school do not 
have to do a 90 probationary period while someone you hire from the outside 
does? Man, could I have fun from a HR perspective on unequal treatment of new 
hires.


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise 
[sscal...@beaumonthospitals.com]
Sent: Friday, February 20, 2009 7:00 PM
To: Bill O'Donnell; Esther Peters
Cc: Histonet
Subject: Re: [Histonet] Practical Exam

It is up to each individual institution to test a new hire for their 
competency.  We are lucky to have both an HT and HTL program at our hospital so 
when we hire a graduating student we already know their work habits and skill 
level.  If I do hire someone from the outside, they have 90 days that they are 
in a probationary period.  If during this 90 day period they have not proven 
their ability to complete the required work according to our standards, they 
could be let go or I have the option to extend the probationary period.  
Whether they have completed a practical exam or not, they still need to prove 
they are competent after they are hired.
We have had to hire from the outside (yes it is a pain going through this 
process) and you hope that your new hire is as competent as they claim to be.  
But in the end you will be the one stuck with an incompetent employee if you 
don't test them and catch it before their probationary period ends.  Whether 
or not they have taken a practical exam really does not matter in the end, 
almost anyone can redo a stain enough times to get it right.  The real question 
is, can they do it right on a daily basis and in a timely manner?

Sharon E. Scalise, HTL (ASCP)
Histology Supervisor
William Beaumont Hospital
Royal Oak, MI 48073
248 898-5981
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[Histonet] RE: Disinfecting Cryostat

[Podawiltz, Thomas]

I believe CAP recommends 70% etoh.


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Louise Hartson 
[louise_hart...@urmc.rochester.edu]
Sent: Friday, February 06, 2009 10:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Disinfecting Cryostat

Can someone suggest a good universal method for disinfecting the Cryostat
after each use?
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[Histonet] RE: Frozen section staining line

[Podawiltz, Thomas]

We have both the line and jars labeled.


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Johnson 
[ajohn...@aipathology.com]
Sent: Tuesday, January 20, 2009 12:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen section staining line

How are labs out there labeling their frozen section staining line?  Do
you label each coplin jar individually

or can there be some way to combine the labeling?



Thanks

Amy Johnson

Associates in Pathology

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[Histonet] RE: Re: As Thanksgiving Approaches

[Podawiltz, Thomas]

Vinnie, its all in how the Physicians explain it to the patients and state 
requirements, it has never been an issue, yet. But you know how that goes, 
haven't meant a hospital lawyer that has not rolled over when push came to 
shove.


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: Della Speranza, Vinnie [EMAIL PROTECTED]
Sent: Tuesday, November 25, 2008 10:29 AM
To: Podawiltz, Thomas; Judy Collins; Histonet@lists.utsouthwestern.edu
Subject: RE: Re: As Thanksgiving Approaches

Tom, I'd normally take this approach but if push came to shove I don't believe 
it would hold up in court. That may depend on the language in your surgical 
consent signed by the patient but that aside, the cost of responding to a 
patient's legal action would be much greater than the small effort it takes to 
render the specimen safer to turn over.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974


-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Podawiltz, Thomas
Sent: Tuesday, November 25, 2008 9:51 AM
To: Judy Collins; Histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Re: As Thanksgiving Approaches

Once specimens arrive at our lab, they are ours. We do not give anything back 
to the patient. No legal liability that way.

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: [EMAIL PROTECTED] [EMAIL PROTECTED] On Behalf Of Judy Collins [EMAIL 
PROTECTED]
Sent: Tuesday, November 25, 2008 9:37 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: As Thanksgiving Approaches

One thing to keep in mind about Vinegar, however, is that fungus will still 
grow in it after a while.  Kind of gross!

Judy Collins
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RE: [Histonet] embedding

[Podawiltz, Thomas]

I would say 2 to 4 hours, depending on how they are submitted. Were biopsy bags 
or foam pads used?, size of pcs.


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: [EMAIL PROTECTED] [EMAIL PROTECTED] On Behalf Of KELLY BOYD [EMAIL 
PROTECTED]
Sent: Thursday, November 06, 2008 1:25 PM
To: histonet
Subject: [Histonet] embedding

Just trying to get some feedback on how much time (estimated) it should take an 
average histotech with experience to embed 150 shave biopsies. Let us just say 
the majority have multiple pieces to be embeded on edge. Any clue?


Kelly D. Boyd, BS, HTL (ASCP)
Lab Manager
Harris Histology Services
2025 Eastgate Dr. Ste. F
Greenville, NC 27858
www.harrishisto.com

Tele (252)-830-6866
Cell  (252)-943-9527
Fax  (252)-830-0032





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RE: [Histonet] Presidential Voting Infomation

[Podawiltz, Thomas]

And this deals with the sharing of Histology ideas How?

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: [EMAIL PROTECTED] [EMAIL PROTECTED] On Behalf Of Edwards, R.E. [EMAIL 
PROTECTED]
Sent: Monday, November 03, 2008 11:19 AM
To: '[EMAIL PROTECTED]'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Presidential Voting Infomation

Thanks but no thanks..

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Justin Thomas
Sent: 03 November 2008 16:11
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Presidential Voting Infomation

Barack Obama will raise taxes on hardworking Americans to give a government 
handout to the 40% of Americans who pay no income taxes.
John McCain and Sarah Palin have an economic plan that celebrates the American 
dream of opportunity, not government giveaways. In this country, we believe in 
spreading opportunity, for those who need jobs and those who create them. While 
Barack Obama is ready to spread the wealth around, John McCain has a plan to 
get our economy moving so everyone has access to good jobs, a quality education 
and the opportunity to succeed.

The next President won't have time to get used to the office. America faces 
many challenges here at home, and many enemies abroad in this dangerous world. 
We cannot spend the next four years as we have spent much of the last eight: 
hoping for our luck to change at home and abroad. We need a new direction, and 
John McCain and Sarah Palin will fight for it.

Time and time again this team of mavericks has stood up, taken on tough issues 
and delivered. They're the real deal. They have a clear record that can deliver 
results, not just rhetoric that delivers votes.


PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK 
ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE.  THIS IS 
A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE 
AHEAD OF US WITH JOHN LEADING THE WAY!!!



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RE: [Histonet] ASCP HT maintenance fees

[Podawiltz, Thomas]

If you were certified in 2004 or after you need to turn in 36 credit hours of 
continuing education in order to maintain your certification. You do not need 
to be a member of ASCP to be certified, however you do get some free CE hours 
with your membership. My certification was in 85, so yes, I am one of the old 
farts that is exempt. However, I have stayed current with my education. even in 
the years that I did not practice Histology.

As a supervisor, I would not look at a resume that had an expired 
certification. Right or wrong I would assume that, the applicant did not take 
this field seriously enough by letting their certification lapse.

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: [EMAIL PROTECTED] [EMAIL PROTECTED] On Behalf Of Kimberly Tuttle [EMAIL 
PROTECTED]
Sent: Monday, November 03, 2008 2:08 PM
To: R C; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] ASCP HT maintenance fees

Really? I never pay to maintain HT certification. As far as I know theres a 
ASCP membership fee, but you dont have to be a member to be certified. Am I 
wrong here?

Kimberly C. Tuttle  HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax


 R C [EMAIL PROTECTED] 11/3/2008 12:49 pm 
Can someone assist me in rationalize the annual cost of maintining HT
certification (roughly $100 annually) and its benefit? Point accumulation is
generally low for classes you must pay for, and those who obtained
certification prior to 2004 are exempt. Should one not pay the annual fee,
certification is dropped Is this correct?). In that case, can one advertise
HT certification for future employment opportunities then, offer full
explanation (and expired certification) during interview and that be
sufficient?

What I generally receive from ASCP is an annual bill and a random newsletter
from time to time. Furthermore, when a bill isn't paid on time,
the termingology in the subsequent bills become similar to that of a
collection agency. Frankly, I find this mailing submission as well as state
and national meetings more informative.

Someone please clarify something I might be missing and any benefits of the
pay out.
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RE: [Histonet] Plus Slides

[Podawiltz, Thomas]

We buy our slides from Mercdes, usually do not have a problem with them
Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: [EMAIL PROTECTED] [EMAIL PROTECTED] On Behalf Of Meghan Tucker [EMAIL 
PROTECTED]
Sent: Monday, September 29, 2008 10:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Plus Slides

Good Morning!

I was wondering where labs purchase their Plus slides from and also if anyone 
is having trouble with debris on their slides?  We have been having a lot of 
trouble with dirty slides right out of the box.

Thanks!

Meghan Tucker




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RE: [Histonet] FEDERAL GOVERNMENT LABS

[Podawiltz, Thomas]

Unless its changed over the years, My Naval lab in Virgina was both JCAHO and 
CAP inspected. Of course I am going back 20 years.

Tom Podawiltz, HT (ASCP)
 
From: [EMAIL PROTECTED] [EMAIL PROTECTED] On Behalf Of TIMOTHY MALLOY [EMAIL 
PROTECTED]
Sent: Friday, September 26, 2008 4:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FEDERAL GOVERNMENT LABS

Does anybody know if the Veterans or Government labs are CAP, JCAHO, AABB, 
ASCP, are Accedited, and if you are a employee how do you keep up an ascp cert. 
with no in house educational credits.


TM. HT ( ASCP ) A.A.S.
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