[Histonet] RE: Tissue falling off

[Susan.Walzer]

Tissue that has been properly processed, cut thin and dried well should not be 
falling off.. We have never needed any additive in our water baths but if we 
have  tough bones or very bloody tissue we use plus slides. PS: we soak tough 
or bloody tissue in soapy water with some ammonia as it REALLY makes a 
difference.

Susan Walzer HT(ASCP)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Friday, August 15, 2014 1:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Tissue falling off

Hi - Just a couple of suggestions -
I've seen this periodically crop up during the years.  There are a couple of 
things to consider:
Water baths are completely clean - after dumpling water and removing paraffin 
debris with whatever you use - wipe out the bath with clean absolute alcohol.
Be careful where you spray - make sure that if you use anything like Paraguard, 
or similar stuff that keeps paraffin from sticking, that it is used sparingly, 
or preferably, not at all.  We had a situation where we had a trial sample that 
someone was using to clean her microtome and forceps and the stuff got 
everywhere and tissues started falling off.
Your water in the waterbaths is clean - Fresh DI water.  Once we had a 
contaminant in our DI water, and the patient tissues slid right off our slides. 
 We use clean slides, good quality water and super clean waterbaths.
Is your heater up and running? - Our slides are stained on a stainer with built 
in slide dryers.  We used to have a problem with the first rack of patient 
slides' tissue falling off because the slide dryer was not hot enough for our 
short dry cycle.  We now send a rack through first, which turns on the heaters, 
then load the patient rack.
Hope this gives you a few ideas
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

   7. tissue falling off slides (Burnett, Brandy)   
From: Burnett, Brandy bburn...@capecodhealth.org
Subject: [Histonet] tissue falling off slides

Our lab has encountered an issue with tissue falling off of the slides.
It is mostly the patient tissue
that is falling off. Some of the Techs are using control slides that have been 
pre-cut and I am wondering if this might be part of the problem. Any 
information would be very much appreciated.
Thanks Again,
Brandy Burnett, HTL


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RE: [Histonet] biopsy bags for processing - alternatives

[Susan.Walzer]

When you open nylon bags they sometime pop the biopsy out and you can lose 
it.  Tiny tissue gets stuck in the the seams of  the bags. Blue sponges made 
artifact indents in tissue. We use round Obex papers and have never lost a 
biopsy. They are wonderful! You can fold them like filter paper ,use a small 
funnel and pour tiny bits of tissue thru catching everything.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Wednesday, August 13, 2014 1:18 PM
To: Timothy Morken
Cc: Histonet
Subject: Re: [Histonet] biopsy bags for processing - alternatives



We also use the nylon bags and have to pay extreme attention when embedding.  
We no longer use sponges due to cross contamaination.  We use to buy tissue 
bags from Fisher and they were similar to the blue paper we use from Leica.  
I have been looking at tea bag vendors (like we get Lipton and Salda tea in).  
They are paper the only issue is the quantity you have to purchase.  Mopec make 
a bag but the issue the open end is even and this is a real pain when trying to 
get inside, as I mentioned before the old Fisher bags had one end longer than 
the other so you were able to place your forceps inside with ease.  Another 
reason I would like to replace the nylon bag is that when you get an aggregate 
of tissue some of it gets inside the mesh and if there is minimal tissue I fear 
you may miss some diagnostic material. 



Sue PaturzoTJUH 




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RE: [Histonet] On the lighter side...

[Susan.Walzer]

Also heard Histotechs are good embed!!! LOL

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: Monday, August 11, 2014 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...


Old histologists never die, they are just well fixed...
Claire

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones 
[mjo...@metropath.com]
Sent: Monday, August 11, 2014 9:07 AM
To: Edwards, Richard; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...

25 years, (what¹s that in micron¹s?²) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) 
Histology Manager Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/11/14, 5:17 AM, Edwards, Richard r...@leicester.ac.uk wrote:

Sniffed my  first formalin and  saw  first post-mortem November 1965.

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RE: [Histonet] On the lighter side...

[Susan.Walzer]

For me 36years..will not be 37, I retire in 3 weeks! Off to visit my son 
and family in England!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar
Sent: Friday, August 08, 2014 11:01 AM
To: Elizabeth Chlipala
Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard
Subject: Re: [Histonet] On the lighter side...

3 years in histology, 2 years registered!

Beth Brinegar HTL (ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403

Beth Brinegar HTL(ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403


On Fri, Aug 8, 2014 at 9:53 AM, Elizabeth Chlipala l...@premierlab.com
wrote:

 Too funny Richard - you would pass with flying colors

 For me its 31 years - HTL-930

 I have really been blessed, I love my job and I have really enjoyed my 
 career.  Every day there is something new to learn or to work on, for 
 example the lab is putting the final touches on a poster that will be 
 displayed at NSH this year, working on that has been exciting and a 
 lot of fun too.

 Liz

 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO 
 Box 18592 Boulder, CO 80308
 (303) 682-3949 office
 (303) 881-0763 cell
 (303) 682-9060 fax
 l...@premierlab.com

 Ship to address:

 Premier Laboratory, LLC
 1567 Skyway Drive, Unit E
 Longmont, CO 80504
 
 From: histonet-boun...@lists.utsouthwestern.edu [ 
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, 
 Richard [ richard.car...@hhchealth.org]
 Sent: Friday, August 08, 2014 8:32 AM
 To: Douglas Porter; histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] On the lighter side...

 36 years; however, not registered.   Hopefully, I can take the QIHC
 someday and pass.

 Richard

 Richard W. Cartun, MS, PhD
 Director, Histology  Immunopathology
 Director, Biospecimen Collection Programs Assistant Director, Anatomic 
 Pathology Hartford Hospital
 80 Seymour Street
 Hartford, CT  06102
 (860) 972-1596 Office
 (860) 545-2204 Fax
 richard.car...@hhchealth.org


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter
 Sent: Thursday, August 07, 2014 2:39 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] On the lighter side...

 How long have you been a registered histotech?  36 years here.  You???



 Douglas A. Porter, HT (ASCP)
 Grossing Technician
 IT Coordinator

 Cancer Registrar


 CAP-Lab, PLC
 2508 South Cedar Street
 Lansing, MI 48910-3138

 517-372-5520 (phone)
 517-372-5540 (fax)

  mailto:doug.por...@caplab.org doug.por...@caplab.org

  http://www.caplab.org/ www.caplab.org



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[Histonet] RE: Ideas for a Retiring Pathologist

[Susan.Walzer]

Over the years we saved interesting gallstones, all shapes and sizes...when it 
came time for the head Doc to retire we glued them on a black background board 
in an interesting swirl.. had it framed on a really nice shadowbox.. It looked 
beautiful.. Weird but beautiful!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Tuesday, July 01, 2014 11:30 AM
To: Disher Lori; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Ideas for a Retiring Pathologist

Depends on the pathologist.   :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
lori.dis...@hcahealthcare.com
Sent: Tuesday, July 01, 2014 11:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ideas for a Retiring Pathologist

I'm looking for creative ideas to celebrate a retiring pathologist sometime 
this year.  What have you all done in the past?

Lori Disher


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RE: [Histonet] Microtome

[Susan.Walzer]

Leica 2125

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Monday, June 02, 2014 10:44 PM
To: Sylvia Shockey
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome

Leica all the way 2025

Sent from my iPhone

 On Jun 2, 2014, at 5:19 PM, Sylvia Shockey 
 sylvia.shoc...@clinicalpathologyassoc.com wrote:
 
 
 Hello,
   My name is Sylvia Shockey I work in a histology lab. One of 
 our microtomes is not working properly and would appreciate some advice on 
 purchasing a new one or maybe refurbished.  I have had some quotes on a 
 Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon 
 Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has 
 new and used on both of these models. Help me please I don't want to buy a 
 clunker.  Thanks for your time.
 
 
 
 
 
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[Histonet] RE: Tissue Tek VIP 5 cleaning cycle

[Susan.Walzer]

Amen!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Wednesday, May 21, 2014 4:23 PM
To: Clements, Mary Ann; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Tissue Tek VIP 5 cleaning cycle

The cleaning cycle is not dependent on the number of blocks you process. Even 
if you process one block the processor has sucked up all the reagents and the 
paraffin into the retorty and has to go thru a clean cycle to clean out the 
lines and the valves to prevent them from getting a paraffin clog. The VIP has 
a built in minimum amount of times that the xylene and the alcohol (and water 
if you use it after the alcohol) have to get sucked up into the retort and spit 
out through the lines and valves. This is to make sure any paraffin residue is 
dissolved. This is also the reason the VIP alerts you after every five clean 
cycles so that you can change the solutions because they may be saturated with 
paraffin and could leave some behind in the lines. Trust me - you do not want 
to clog up your processor. Not fun to clean it out. I, myself, have never done 
that but in another life I was in sales and I saw some awful things that people 
did to their processors. 

Andi

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Clements, Mary Ann 
[cleme...@evms.edu]
Sent: Wednesday, May 21, 2014 12:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Tek VIP 5 cleaning cycle

Please excuse my ignorance as I am new to the world of Histology!
We currently run a 96 minute cleaning cycle after processing only 10-30 blocks. 
 Is it feasible to run a 60 minute cleaning cycle since our volume is so low or 
is length of cleaning cycle independent of block count?
Thank you in advance for your expertise,


Mary Ann Clements, B.S.
Biorepository Manager
Eastern Virginia Medical School
Leroy T. Canoles Jr. Cancer Research Center Lewis Hall, Room 3018
700 West Olney Road
Norfolk, VA  23507

email: cleme...@evms.edumailto:cleme...@evms.edu
phone: (757) 446-7910
fax: (757) 446- 7059

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RE: [Histonet] Basement Lab

[Susan.Walzer]

Why do they always want to put us in the basement? We have a lot of the 
hospitals explosives and flammables??
Still, as long as they have a good ventilation (you often need a roof or 
outside wall to do this) it might be ok. I hope they have good environmental 
engineers.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Saturday, May 03, 2014 3:51 PM
To: John Smallwood
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Basement Lab

Not a good idea

Sent from my iPhone

 On May 3, 2014, at 12:10 PM, John Smallwood jrsmallw...@bell.net wrote:
 
   Our small Hospital with growth plans, is considering a new Laboratory in 
 the basement of the planned tower. I consider this a less than desirable 
 location. Spills , fumes, chemical allotments etc. What are Histonet members 
 thoughts and ideas ??
 
 Than you,
 John Smallwood, MLT.
 London, Ont. Can.
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[Histonet] RE: Tissue Requisition

[Susan.Walzer]

Our req's come over the computer , not with specimen. We then check the req 
against the label on the specimen.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Monday, March 24, 2014 5:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Requisition

With the constant changes in our CAP checklists and the changes in electronic 
ordering and moves to go paperless a question has come up that I know used to 
be in the checklists but I can't seem to find it now. In the past surgical 
pathology specimens had to be sent with a requisition.  Our hospital is 
reviewing our process for handling specimens from various hospital departments. 
  Currently we still require that a requisition is brought with the specimen.  
We have a system  where the various OR staff could place an electronic order 
for a surgical specimen in the computer and then the paper requisition will 
then print in the surgical pathology grossing area.   We are trying to decide 
if  a copy of the requisition still needs to accompany the specimen when it is 
delivered to the grossing room.   The specimens have electronic labels on them 
with patient identifiers and the exact site of the specimen is also listed on 
the label.  Please tell me how others are handling these changes.   Obviously I 
can see some advantages to no longer have illegible hand-written reqs from the 
OR docs or nurses, but something bothers me that a specimen will arrive without 
a requisition.   Any thoughts?

Jim

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor Memorial Medical Center
217-788-4046



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RE: [Histonet] What is a level?

[Susan.Walzer]

10 turns of the wheel is fine for most tissue however we often get FNA's that 
are so tiny that several turns of the wheel and you will be completely through 
it. It is often a judgment call by the tech.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Monday, March 17, 2014 5:58 PM
To: Bruce Gapinski; histonet
Subject: RE: [Histonet] What is a level?

You should ask the pathologist what their definition of a level. My 
dermatopathologists want 40 microns (10 rotations of the microtome wheel, 
cutting at 4 microns) for a level. This is the typical size of most organells 
they are looking at. Again, the level depth should be discussed and agreed upon 
by the pathologist and microtomist. This takes the guess work out of the 
process. once you have a specific description, make sure this is added to your 
SOP.

William DeSalvo, BS HTL(ASCP)
 
 From: bgapin...@pathgroup.com
 To: histonet@lists.utsouthwestern.edu
 Date: Mon, 17 Mar 2014 21:19:40 +
 Subject: [Histonet] What is a level?
 
 What is a level?
 Levels, deepers, we all call it something different, but what exactly is a 
 level?  I know it is all relative. One would never cut into a prostate needle 
 biopsy the same way they'd cut into a skin.
 But if we suppose are tissue sample is 3mm thick (don't laugh). How deeply 
 would you cut when the pathologist asks for a level? I guess I'm talking to 
 those of us with automated microtomes where we can set the trim to 10, 20, 30 
 microns. I started on a manual microtome where it's impossible to gauge this 
 altogether.
 So let's say I have a nicely processed ellipse of skin grossed 3mm thick.  
 The pathologist asks me for a level. If we assume I gave them a full face on 
 the first slide, how much deeper should I go to get the level? 60-80 microns? 
 Deeper? Less?
 Your thoughts please,
 
 
 Respectfully,
 Bruce Gapinski HT (ASCP)
 
 
 
 
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RE: [Histonet] GI Biopsies

[Susan.Walzer]

We do 3 levels on all..taking  hp immuno on 2nd level of gastrics and a couple 
of extra on esophs. ( in case of alcian blues or pas/fungus).

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
imhype...@aol.com
Sent: Thursday, March 13, 2014 2:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GI Biopsies


Good afternoon all,
 I was just curious about how your institutions handle GI biopsies, 
specifically how many slides you cut off the bat.  We presently cut 2 levels on 
each GI biopsy block, but I'm hearing that more and more places only cut 1 
slide per GI biopsy block.  Please share what you are doing at your 
establishment.
Thank you 
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[Histonet] RE: Low vs. High Profile Blades

[Susan.Walzer]

We use the low profile for everything...Accu-Edge. They are superior.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor 
Jordan
Sent: Friday, March 07, 2014 4:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Low vs. High Profile Blades

Hey does anyone know the difference between a High Profile Blade vs. a Low 
Profile Blade when using for sectioning of Paraffin embedded tissues specimens? 
Which one would ya'll prefer for decalcified bone?


Trevor Jordan Wait
University of Texas Health Science Center, San Antonio Class of 2017 MD 
Candidate Abilene Christian University Class of 2013 Graduate B.S.  
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[Histonet] RE: Slides for control/patient

[Susan.Walzer]

We like Fisher's Red Control Slide with Adhesion #22-042-910

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, February 28, 2014 9:58 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slides for control/patient

Happy Friday!
I was wondering, for the labs that are using the slides for IHC that have a 
place for the control tissue and the patient tissue, which vendor would you 
recommend. I also would not mind being contacted directly by vendors who wish 
to inform me of their products.

Thanks,
Toni
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RE: [Histonet] Soaking artifact

[Susan.Walzer]

Incredible! Lee Luna (who wrote the book on histology) always said rehydrate! 
rehydrate, rehydrate Where are these so called supervisors coming from??? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of b427...@aol.com
Sent: Sunday, January 05, 2014 9:41 PM
To: dea.les...@gmail.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Soaking artifact

Your supervisor is wrong, and inexperienced.  What artifact?  Some tissues MUST 
be soaked on wet ice, spleen, liver, eye lens, anything bloody -   You just 
can't get quality sections without soaking some tissues. You can tell her I 
said so.  I'd like to know what artifact she is 'seeing'.
Jackie O'Connor



-Original Message-
From: Deanna Leslie dea.les...@gmail.com
To: histonet histonet@lists.utsouthwestern.edu
Sent: Sun, Jan 5, 2014 3:44 pm
Subject: [Histonet] Soaking artifact


Has anybody in histoland ever heard of this?  I have been cutting tissue for 25 
yrs and until recently I had never heard of this!
I am under contract to a facility and the supervisor there does not want 
anybody to soak their tissue or use ice!  Your are supposed to use the cold 
plate, because as I have stated soaking them causing an artifact. I have not 
disputed this because it is not my place or in my job discription as a 
traveler.  I am not even sure what it is supposed to look like or what type of 
problems it causes.

Thanks for listening!
Deanna Leslie HT ASCP
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RE: [Histonet] Faxitron Xray digital imaging

[Susan.Walzer]

Right, it's the only way to be sure bone is really decaled enough. We also used 
to use it to find calcium in lumpectomies. I am sure we were not doing proper 
monitoring of it though as it was in our dept. and radiology never checked it. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
pru...@ihctech.net
Sent: Wednesday, November 06, 2013 9:39 PM
To: 'WILLIAM DESALVO'; 'Scott, Allison D'; 'histonet'
Subject: RE: [Histonet] Faxitron Xray digital imaging

Years ago/maybe more than 25 we used a faxitron xray machine to measure bone 
calcification on research samples and samples being decaled, it was the best 
thing since sliced bread, had to go to the radiology dept to use it and was 
told they were dying off the market, loved it, so easy to use and so useful.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
rueggihcconsultin...@outlook.com



 

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
Sent: Tuesday, November 05, 2013 9:47 AM
To: Scott, Allison D; histonet
Subject: RE: [Histonet] Faxitron Xray digital imaging

We have used the Faxitron instrument for the past 3 years at multiple sites.
Faxitron creates a digital image of a specimen quickly and safely and a t
multiple magnifications. The instrument is placed in either Surgery or
Surgical Pathology (SP) and we use it primarily on breast cases to assist in
orientation and gross dissection, identifying radioactive seeds or locating
micro calcification. The instrument is easy to use, safe and you can connect
to multiple LIS systems and a Hospital PACS. There is no code to charge for
use when used in SP, only when used by Radiology or Surgery.
 
There is only one company that manufactures and sells the instrument:
Faxitron Bioptics, LLC
Tucson, AZ
877-910-0030
 
The are willing to bring a unit to your site for demonstration and you may
be able to talk them into leaving for a evaluation.
William DeSalvo, BS HTL(ASCP)

 
 From: allison.sc...@harrishealth.org
 To: histonet@lists.utsouthwestern.edu
 Date: Tue, 5 Nov 2013 16:00:11 +
 Subject: [Histonet] Faxitron Xray digital imaging
 
 Hello to all in histoland.  Our chief pathologist is interested in getting
a faxitron xray digital imaging machine.  Is anyone out there using this
technology and can you suggest a company in which to order one from.  Any
help in this will be greatly appreciated.
 
 
 
 
 Allison Scott HT(ASCP)
 Supervisor, Histology Lab
 LBJ Hospital
 Harris Health System
 Office: 713-566-2148
 Lab: 713-566-5287
 
 
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RE: [Histonet] Unregistered HT

[Susan.Walzer]

I had a pathologist who said we were all  laboratory scientists, now if the 
pay reflected that

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Wednesday, September 11, 2013 9:58 AM
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unregistered HT

Do you get paid more with that title? Just curious, I'm not sure what 
laboratory professional means, as I'm not in that kind of lab.
Or is it a respect thing?

Emily

By bitching and bitching and bitching, they could exhaust the drama of their 
own horror stories. Grow bored. Only then could they accept a new story for 
their lives. Move forward.

-Chuck Palahniuk, Haunted


On Tue, Sep 10, 2013 at 7:32 PM, Jennifer MacDonald jmacdon...@mtsac.eduwrote:

 As long as we do not need certification, licensure and minium 
 education requirements we will not be recognized as Laboratory Professionals.


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RE: [Histonet] Blade Rationing Follow-up

[Susan.Walzer]

You can shop all the blades in the world( and we have tried many) but if you 
care about quality sections the Accu-edges are the only game in town. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper
Sent: Wednesday, June 19, 2013 3:20 PM
To: 'Teresa Moore'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Blade Rationing Follow-up

You could start with cost comparison of blades (since this whole thing started 
as a blade issue).  Reps are willing to let you demo some before committing and 
you might save money if everyone likes a less expensive blade.  You can do the 
same for paraffin and slides as well as reagent alcohols and xylene.  Again, 
these are things the manager should be getting after.  Does your lab adhere to 
Lean principles?  There may be some cost savings there if you can accomplish 
tasks more efficiently; get the work done in less time or cutting back on 
overtime.  It's a bit difficult to say as much depends on the nature and scope 
of your service.  All situations are unique - do you send a lot of work out?  
Could you take it on in-house?  Or are you trying to do things in-house that 
could be sent out and have a cost positive effect?  Lots of questions for the 
manager...

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teresa Moore
Sent: Tuesday, June 18, 2013 1:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blade Rationing Follow-up

I really appreciate everyone's constructive comments regarding my post on blade 
rationing.  Lots of you said there are many other ways to cut costs in the lab. 
 I would like to hear some of your suggestions so I can take them back to my 
manager.  I'd like to give her some legitimate alternatives to her proposal. 
Would like to contribute to solving the problem of cutting costs.

Thanks again



Teresa Moore, HT
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RE: [Histonet] Blade Rationing

[Susan.Walzer]

You need to tell your manager that you cannot do your job without proper tools. 
Only the tech cutting knows how many blades he or she needs to cut a days work. 
These micro managers need to do some bench work and get a reality check. 
Unbelievable! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Monday, June 17, 2013 5:49 PM
To: Teresa Moore
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Blade Rationing

From a managers point of view, whoin my opinion that is a poor way to try to 
cut expenses. It will only lead to recuts and possible loss of important 
tissue. For the techs to understand the necessity to conserve is important but 
the tech needs to use their discretion as to when a blade needs changing.  

Sent from my iPhone

On Jun 17, 2013, at 5:08 PM, Teresa Moore tmoor...@gmail.com wrote:

 I work in a hospital, there are three of us on this particular shift 
 and we cut approx. 200 blocks, give or take a few.  Our histo lab 
 manager is telling us we should only be using one pack of blades (50 
 per pack) a month.  I'm wondering what other techs think of this 
 especially lab managers and supervisors.
 
 tmoor...@gmail.com
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RE: [Histonet] Re-use of plastic embedding molds

[Susan.Walzer]

We tried them but they have a tendency to warp and you will not get a flat 
surface for cutting.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Thursday, May 09, 2013 2:04 PM
To: Myers, Karyn; histonet
Subject: RE: [Histonet] Re-use of plastic embedding molds

We tried using the disposable molds and they work for reusing  2-3 times( 
cracks started after just a few uses), but we found the cost much higher that 
using the metal molds and cleaning.

William DeSalvo, BS HTL(ASCP)
Production Manager-Anatomic Pathology
Chair, NSH Quality Management Committee
Owner/Consultant, Collaborative Advantage Consulting

 

 From: karynmy...@texashealth.org
 To: histonet@lists.utsouthwestern.edu
 Date: Thu, 9 May 2013 17:26:13 +
 Subject: [Histonet] Re-use of plastic embedding molds
 
 Just wondering... Does anyone re-use their plastic embedding base molds?
 
 Thanks for your input!
 
 
 
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[Histonet] RE: Histonet Digest, Vol 112, Issue 9

[Susan.Walzer]

PS.. Even with the clamp problem I think the Leicas are superior.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth 
A (NIH/NIDCR) [E]
Sent: Friday, March 08, 2013 2:01 PM
To: 'Weaver, Stephanie'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9

Stephanie,
This good to know because I'm considering a new microtome.
Ruth
N.I.H.

-Original Message-
From: Weaver, Stephanie [mailto:swea...@tvmdl.tamu.edu] 
Sent: Friday, March 08, 2013 1:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9

We purchased a Leica RM2125RMS manual rotary microtome new from Leica less than 
a year ago and you may want to be aware of the issues that we are having.  We 
purchased it for multiple reasons, including the excellent reputation of Leica 
microtomes and the great sectioning of tissues (even bone) that we experienced 
during the demo.  It still cuts great sections but I am not pleased with this 
purchase.  We have replaced the cassette clamp at least 6 times since we've 
purchased it because the solid steel level to pull to open the cassette clamp 
breaks.  Each new cassette clamp seems to last less time than the last.  We are 
still under warranty so they have replaced this part for free every time and 
they are always kind when I tell them it broke again, but this is just not 
acceptable.  We have been told that it is a known manufacturing problem and 
that they are working on a resolution but I would still be wary since this is 
obviously not fixed yet.  The body of the microtome is also already showing 
discoloration and signs of corrosion but since these parts do not move and are 
not under stress it does not seem to be affecting the microtome.  At this point 
I don't think I would ever buy Leica again, but I know that a lot of people 
have loved their equipment and have had very good longevity out of it.

Good luck,
Stephanie Weaver, HT (ASCP)

From: kgrob...@rci.rutgers.edu kgrob...@rci.rutgers.edu
To: histonet histonet@lists.utsouthwestern.edu
Sent: Thursday, March 7, 2013 2:32 PM
Subject: [Histonet] Microtome upgrade, planning stages...

We are starting to look into upgrading to higher-end, but used, microtomes.? 
What we have is still working, but the writing is on the wall for them in terms 
of repair/parts.

Which brands/models are considered higher-end in the used market these days?? 
(Leica is already on my list, but which models?)

Thanks so much!

Kathleen


Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology  Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(848) 445-1443
FAX (732) 445-6905


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[Histonet] RE: Histonet Digest, Vol 112, Issue 9

[Susan.Walzer]

The problem seems to be that the clamp screw handle is welded on and that is 
the weakness. On the old tall Leicas (does anyone remember them?) the  screw-in 
lever was solid and we never had this problem. Also the springs get rusty 
inside the clamps probably from corrosive surface decal. It seems to be an 
ongoing problem.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bouchal, Rena L
Sent: Friday, March 08, 2013 2:08 PM
To: 'Weaver, Stephanie'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9

We have had the same experiences with the clamps. At one point we were told we 
had to purchase a complete $2000 head when one of our clamps went. We got 
ugly and they gave it to us for free, but it was not pleasant. We have several 
Leica's and I am going to look at a Microm this year because of these problems. 
No one has ever admitted to us that there is a known manufacturing problem. 
Its too bad because other than that we have been quite pleased with the units. 
I just don't want to fight with the company routinely over clamps.  

Please note that my email address  as of Jan 3, 2011 is 
bouch...@wvuhealthcare.com  .   Please make the appropriate changes in your 
address book.

Rena Bouchal, M.S.
Anatomic Pathology Manager
West Virginia University Hospitals
304-293-7765

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weaver, 
Stephanie
Sent: Friday, March 08, 2013 1:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9

We purchased a Leica RM2125RMS manual rotary microtome new from Leica less than 
a year ago and you may want to be aware of the issues that we are having.  We 
purchased it for multiple reasons, including the excellent reputation of Leica 
microtomes and the great sectioning of tissues (even bone) that we experienced 
during the demo.  It still cuts great sections but I am not pleased with this 
purchase.  We have replaced the cassette clamp at least 6 times since we've 
purchased it because the solid steel level to pull to open the cassette clamp 
breaks.  Each new cassette clamp seems to last less time than the last.  We are 
still under warranty so they have replaced this part for free every time and 
they are always kind when I tell them it broke again, but this is just not 
acceptable.  We have been told that it is a known manufacturing problem and 
that they are working on a resolution but I would still be wary since this is 
obviously not fixed yet.  The body of the microtome is also already showing 
discoloration and signs of corrosion but since these parts do not move and are 
not under stress it does not seem to be affecting the microtome.  At this point 
I don't think I would ever buy Leica again, but I know that a lot of people 
have loved their equipment and have had very good longevity out of it.

Good luck,
Stephanie Weaver, HT (ASCP)

From: kgrob...@rci.rutgers.edu kgrob...@rci.rutgers.edu
To: histonet histonet@lists.utsouthwestern.edu
Sent: Thursday, March 7, 2013 2:32 PM
Subject: [Histonet] Microtome upgrade, planning stages...

We are starting to look into upgrading to higher-end, but used, microtomes.? 
What we have is still working, but the writing is on the wall for them in terms 
of repair/parts.

Which brands/models are considered higher-end in the used market these days?? 
(Leica is already on my list, but which models?)

Thanks so much!

Kathleen


Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology  Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(848) 445-1443
FAX (732) 445-6905


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RE: [Histonet] streaks

[Susan.Walzer]

Many vendors are pushing different blades...but the only blade that is superior 
is the Accu-edge. If you are using any other you will very likely get inferior 
sections. That is 30 years of experience using Accu-edge blades always trying 
others and always going back to the best.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner
Sent: Tuesday, February 26, 2013 12:34 PM
To: cthorn...@dahlchase.com; Histonet
Subject: [Histonet] streaks

Hi Claire,
 
I may be grasping at straws here, but I went to an NSH workshop once where this 
very question was posed.  You won't believe what the cause was.  They had a bad 
lot of blades.  The whole lot was nicked, so changing to a new knife or a new 
box of knives did not help the problem.  Changing the lot did the trick.  I 
don't know if this is your problem or not, but I thought it was very 
interesting.
 
Sheila HT (ASCP)
Knoxville, TN
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[Histonet] RE: CAP checklist question

[Susan.Walzer]

We put in a procedure stating that should we change the schedule we will test a 
variety of tissue (10 different types) and have the pathologist assess the run. 
We have not changed a schedule in years but we seek to comply...

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
Sent: Monday, February 25, 2013 1:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP checklist question

Hi gang..

I am working on a self-inspection CAP checklist and came across the following:

ANP.23120   Tissue Processor

Tissue processing schedules are validated.   Note: New tissue 
processing schedules must be validated against the standard laboratory 
processing schedule.

  Evidence of compliance:   Written procedure for validation of new tissue 
processing schedules AND
 WC records documenting validation

What do you all make of this??  We have not had any new processing schedules 
introduced into our lab in years. So, I really don't know how to proceed on 
this one.
Any insite will be greatly appreciated.

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com


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[Histonet] RE: Sonority Question

[Susan.Walzer]

I would have to consider the tech who is working continuously as senior. That 
is the tech that I would rely on the most.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Tuesday, February 12, 2013 4:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Senority Question

I have 2 employees that are wondering about seniority... one HT has been on 
payroll for 5 years and has gone from FT to PRN and back to FT without any 
breaks.  The other tech has been FT for 3 years. Which one is the senior in the 
lab?


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[Histonet] RE: Processor dehydration cycles..

[Susan.Walzer]

70,80 95,95,100,100

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of PRESZLER, 
JEREMIAH C MSgt USAF AETC 59 LSQ/SGVLH
Sent: Thursday, February 07, 2013 11:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: Processor dehydration cycles..


I agree with Joyce on this: Formalin salt precipitate tends to become more 
common if you start above 70%. WE use a 70%, then 80% and two 95% in our 
process here.


Very Respectfully,

Jeremiah C. Preszler, MSgt, USAF HT (ASCP) Flight Chief, Anatomic Pathology
959 CSPS/ SGVLH
WHASC
JBSA-Lackland AFB, TX 78236
(210) 292-5519
DSN: 662-5519




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[Histonet] RE: Temperatures

[Susan.Walzer]

We have the clinical lab check our friges and freezers on the 
weekends..everything else is off.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sullivan, 
Beatrice
Sent: Friday, February 08, 2013 7:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperatures

I'm looking for a fix to our problem of no temperatures being taken on the 
weekends. We are closed and this is creating an issue. Our processors are not 
running until Sunday night but the paraffin in both the processors and 
embedding center are kept molten. Any help would be greatly appreciated

Beatrice L. Sullivan HT(ASCP)HTL
Corporate Histology Manager
Virtua, Voorhees
856-247-3144


This message, and any included attachments, are from Virtua Health or its 
related affiliates and is intended only for the addressee(s). The information 
contained herein is privileged, proprietary or may include confidential 
information and/or protected patient health information. Any unauthorized 
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or taking any action based on such information is strictly prohibited. If you 
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authorized to receive it, please delete this message promptly and notify the 
sender by e-mail with a copy to issecur...@virtua.org. 

Thank you

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RE: [Histonet] Alcian Blue

[Susan.Walzer]

We've always used 30 minutes and it has never failed.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey
Sent: Thursday, December 06, 2012 8:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Alcian Blue


Hi Everyone:
Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue 
and some say 10 min?Our control works well at 10 minutes but today I had a Dr. 
say that he expected a small amount of cells to stain and they didn't in an 
esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read 
say 30 min, that's what would be best?
Thanks
Sheila
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RE: [Histonet] Tissue Processors

[Susan.Walzer]

VIP always!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, December 04, 2012 3:57 PM
To: Tim Wheelock; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue Processors

I always used VIP because of reliability, toughness and customer service.
René J.

From: Tim Wheelock twheel...@mclean.harvard.edu
To: histonet@lists.utsouthwestern.edu 
Sent: Tuesday, December 4, 2012 3:48 PM
Subject: [Histonet] Tissue Processors

Hi Everyone:

I am currently evaluating three tissue processors.
They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior 
ES.

I was wondering if people could give me their critical opinions and preferences 
on these three machines.
In addition to reliability and ease of use,  I am interested in people's 
experience with tech support, software, or any other factor-positive or 
negative-that prompted your decision.
I currently have a 14 year old Shandon Hypercenter XP.

Thank you,

Tim Wheelock
Neuropathology Laboratory
Harvard Brain Bank
McLean Hospital
Belmont, MA



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RE: [Histonet] RE: Metal molds

[Susan.Walzer]

We put our molds in the VIP before running the cleaning cycle daily. Then we 
dip them in alcohol containing mold release..air dry and store.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Tuesday, October 09, 2012 3:27 PM
To: valerie.han...@parrishmed.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


I always cleaned them daily, either the very hot water, soapy water method, 
with water running over them in the sink with them on their sides so it passes 
over them, not upright so the water sits in them- then a rinse in alcohol and 
completely air dry. Or you can always do the clean cycle with the racks, 
running them through xylene, etc. They come out very clean this way- used an 
old processor that was a backup for this most of the time. But I always did 
them daily, but also wiped each one out with gauze if I used them twice in an 
embedding session ( for more than one specimen in that large batch). Also I 
like metal, I hate those plastic ones. If you keep the block face surface of 
the mold warm-hot,  and flatten before it turns completely white the specimen 
is at the surface and you are able to see the edges easily without a lot of 
facing. I think this saves time cutting through paraffin, and saves blades. 
Plus if the specimen is not flat enough, you see it right away and know if you 
must re-embed to get a complete, representative section, rather than after you 
have cut some superficial parts of some edges away and not others, only to have 
to re-embed anyhow. The other problems I see are when people are afraid of 
big molds- please if you are only taking one section, use one large enough to 
leave a perimeter. Don't try to squeeze it into a medium mold, you are unlikely 
to need multiple sections on one slide and it is much easier to get flat and 
get a good section.  Also please  put enough paraffin on top, so that when it 
is cool the layer over the grooves in the cassette is not so thin that you can 
clearly see the depressions. That little bit of paraffin is much cheaper than 
tech time in re-embedding and fussing with a block longer than you should.  Not 
so much a big issue for many specimens, but anything hard/ dense, such as bone, 
cervix, uterus, leeps, ( you get the idea) it is not anchored enough without a 
good dose of paraffin, causing more chatter when you section, and maybe 
chipping out more frequently, or even the whole bottom surface to lift off the 
cassette. I guess I have some pet peeves with this topic,  so thanks for 
letting me get that out!




Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: valerie.han...@parrishmed.com
 To: billodonn...@catholichealth.net; histonet@lists.utsouthwestern.edu
 Date: Tue, 9 Oct 2012 10:51:01 -0400
 CC: 
 Subject: [Histonet] RE: Metal molds
 
 We clean our molds once a week. Soak them in Xylene to remove paraffin, soak 
 in 100% alcohol to remove xylene, rinse in running water, dry and spray with 
 mold release solution.
 
 Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
 Histology Section Chief
 Parrish Medical Center
 951 N. Washington Ave.
 Titusville, Florida 32976
 Phone:(321) 268-6333 ext. 7506
 Fax: (321) 268-6149
 valerie.han...@parrishmed.com
  
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, 
 Bill
 Sent: Monday, October 08, 2012 4:32 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Metal molds
 
 
  OK folks, I know I should be smarter than this and I haven't seen discussion 
 on it lately 
 
 Are people cleaning their metal embedding molds after evey embedding session?
 
 If not, how often do you clean them? 
 
 Do you clean them at all?
 
 If you clean them, how do you do it? 
 
 Thanks
 
 Bill
 William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan 
 Hospital 10 East 31st Street Kearney, NE 68847 
 
 SERENITY is not freedom from the storm, but peace amid the storm.
 
 Cultivate it in PRAYER!
 
  
 
 
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RE: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray

[Susan.Walzer]

Ice is still the best coolant because it adds moisture. As Lee Luna used to 
say Rehydrate, rehydrate, rehydrate For tough blocks, face it then add soapy 
water to ice with some ammonium hydroxide. Like magic!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, 
Thomas
Sent: Monday, October 01, 2012 2:01 PM
To: joelle weaver; histotech...@gmail.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray

I was once worked at a place that had this sign on the wall that everyone saw 
as you entered the training room.

When you think you are through learning, you are through here

Blows my mind when I run into people that refuse to learn anything current.



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Saturday, September 29, 2012 7:12 PM
To: histotech...@gmail.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray


JennyYou don't need to respond to this, but I will post in case there is anyone 
else out there who is going through the same experiences and feeling 
discouraged.  There are many people in the field like this. I have been out 
there at least a little while and I went through the same response once out of 
histology school and get attitude all the time, still to this day, even though 
I have jumped through all the usual hoops at this point. I can tell you that 
while  working in some labs, I thought of quitting histology almost weekly 
because I got so sick of this kind of thing  People have gotten into the field 
in various ways,  and they sometimes get into ruts and they don't get out there 
much to learn all the new techniques and information. People coming in with 
ideas threaten the status quo,  and sometimes it is just difficult many 
people to change.  I haven't quit histology yet though,   and you shouldn't  
let other people drive you out or make you doubt yourself either. Trust me, we 
need educated and trained people in this field in a desperate way. Look, we all 
have stuff to learn,  new and old! If you stop learning and believe you know 
it all,  you are a real drag to everyone and holding back others who want to 
learn and grow. If you are a newer tech, I think that can be a plus. You are 
fresh and full of enthusiasm and new ideas. The seasoned can share what they 
have learned from time and experience, and you can bring new ideas and your 
fresh enthusiasm and energy- I don't see why that can't be a win-win. Anyhow, 
all of this are just my opinions, and I may get slammed for these comments like 
I have many times before,  but as far as I can tell  from what you have posted, 
 you are not wrong, and your techniques seem reasonable for the hospital 
clinical setting. In addition, you seem to understand why they work  for you 
and the technical rationale behind them. To me this is good. Many people have 
been taught how to do things, but not why.  We need more people who want to 
know why and who care about quality. Please read the other posts about blades ( 
they said it best already) but I feel that is crap about skimping  on them ( 
sorry) . No patient is worth less than any disposable blade. That is why they 
call them disposable. You should not waste supplies, that is irresponsible, but 
within reason you need decent supplies to get the best quality you can possible 
can. Ice is cheap, much cheaper than freeze it spray..(as someone mentioned and 
a good point) .. so there is no valid economic/operational reason there that I 
can see to justify any of that.




Joelle Weaver MAOM, HTL (ASCP) QIHC
 Date: Sat, 29 Sep 2012 18:26:38 -0400
Subject: Re: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray
From: histotech...@gmail.com
To: joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu

Thanks everybody for your answers. I cant respond them all but I concluded that 
the best way to get good sections is too chill the blocks on ice because I 
agree that it facilitates the process.

I really don't understand why my supervisor depends so much on freezing sprays 
to cut and the pathologist has never complained about artifacts caused by them 
but I do believe that they are present because I have seen them getting formed. 
It makes sectioning difficult because you try to get sections free of holes and 
that contributes to the problem.


At my lab is the same thing. My supervisor is in charge of the embedding and 
she just use the ice only for hardening the paraffin block. We don't have a 
standard embedding center with cold plate. Since is a small lab we just have a 
heating plate where we handle the 

RE: [Histonet] DAB precipitate on H. pylori

[Susan.Walzer]

We never had a problem until they changed AB's on us. We sent them slides 
procedures etc to test but in the mean time we tried Cell Marques AB and it was 
clean. Problem solved. Now we all need to demand that Ventana switch back to 
Cell Marques H pylori so that we do not have to pay for those #@*% prep kits.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez
Sent: Monday, August 20, 2012 2:11 PM
To: Dorothy Glass; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] DAB precipitate on H. pylori

Are you using the ventana h. pylori?  If you are we are having that problem 
with some of our satellite labs.  Ventana said its an issue with that new 
antibody and some labs but not all are experiencing this.  Have you called it 
in the Customer support???

Vanessa Perez Garcia
Histology Supervisor
Pathology Reference Lab
210-892-3746
210-892-3732
vpe...@pathreflab.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dorothy Glass
Sent: Monday, August 20, 2012 1:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] DAB precipitate on H. pylori

I have been getting something on some slides, not all that looks like bacteria 
but it is not. It looks like Dab precipitate. It looks like the Dab is breaking 
down,  but only noticeable on the stains for H. pylori.  I wash my water bath, 
and changes my blades. It not occurs all the time, but it has happened about 
four times.  Could it be the water not being filtered properly. We have a water 
filtering system, but it has not been working well lately. Would that have 
anything to do with the brown patches on H. pylori?
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[Histonet] RE: Teabags

[Susan.Walzer]

I was also tired of digging bone marrow particles and biopsies out of the 
stitching. 
Some people like them because they can just dump tissue in them but they do not 
have to fight with them when embedding. Biopsy cassettes can trap air and 
float. The best all around product is Obex round papers. For people who like to 
dump you can fold them into cones and use like filter paper. They are the best 
thing for all around protection of small and friable tissue.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Monday, August 06, 2012 10:37 AM
To: Mayer,Toysha N; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Teabags 

I don't know if this would work for everyone, but we process our hair with skin 
scraping samples (animal source) in lens paper.  We form a small packet with 
all folds on one side of the packet and embed the entire thing with the one 
layer of lens paper down. Keeps everything corralled so to speak.




Tresa Goins
Montana Veterinary Diagnostic Lab
Bozeman, Montana 59718

406-994-6353


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N
Sent: Monday, August 06, 2012 8:10 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Teabags (Contact HistoCare)

You can use teabags to give specimens shape such as EMB and ECC.  Those lose 
particles may not wash through a microcassette, but would just be loose pieces 
in there if not in a bag.  You try to pick up those tiny pieces out of the 
corners.  In a bag you can scrape them off, shape them and put them in the 
mold. Small individual samples such as GI biopsies can go in a biopsy cassette. 
Great question, I am going to add it to my exam for my students.


Toysha N. Mayer, MBA, HT (ASCP)
Instructor, Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnma...@mdanderson.org

Hi all,

Just a curiosity of mine, having contracted for many places I've seen many 
different processes, some efficient and some inefficient. I find a lot of labs 
do what they've always done just because they've always done something a 
certain way for so long whether it's useful or not and generally are not 
interested in change.

One of these things I'm referring to is using teabags. I know some of you LOVE 
them, but there are few things I loathe more than trying to dig out a tiny 
biopsy sample from a teabag along with trying to open it while being stuck 
together by the wax. 

Why in the world would anyone ever use teabags when there are microcassettes 
and even biopsy cassettes?

Please let me hear it.


www.HistoCare.com
Histology Staffing for your Lab



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RE: [Histonet] Slide distribution amongst Pathologists

[Susan.Walzer]

Boy, they sure like to put us in the middle of what should be their own 
problem. Thank heavens I only work now for one Dr at a time now but when I was 
at a larger place they rotated. They still used to tell us to give them 
particular cases when it was not their turn so that we got the flak when 
someone did not get what they thought was theirs. You can never win! :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Monday, June 04, 2012 2:34 PM
To: 'Sheila Adey'
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Slide distribution amongst Pathologists

Here the slides go through the hands of one pathologist, who distributes the
cases. 
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sheila
Adey
Gesendet: Montag, 04. Juni 2012 20:19
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Slide distribution amongst Pathologists


Hello Netters:
I am looking for some ideas regarding slide distribution amongst
pathologists.
Currently one Dr. reads all the surgicals and one other reads the cytology
and bone marrows.
We now have 5 Dr.s and they are looking for ways to disperse the work evenly
per day.
Thanks
:)
 
Sheila Adey
Charge Technologist
Laboratory - Histology Department
Bluewater Health
89 Norman Street
Sarnia, ON  N7T 6S3
519-464-4500 x 7063
 
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[Histonet] Uranyl nitrate

[Susan.Walzer]

Does anyone know a retic stain that does not use uranyl nitrate?
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RE: [Histonet] Fwd: Plants

[Susan.Walzer]

I read an article once that said spider plants absorb formalin fumes we have 
kept them in the histo lab for years.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey
Sent: Friday, May 11, 2012 4:07 PM
To: Victoria Baker
Cc: histonet@lists.utsouthwestern.edu; Behnaz Sohrab
Subject: Re: [Histonet] Fwd: Plants

I keep a pothos and spider plant in my lab. EHS has never complained,
though I can't say one way or another if it's technically allowed.

While my plants are mostly just decorative (I don't think I have enough of
them to make much of a difference), it doesn't hurt that they may be
filtering our air somewhat. NASA compiled a list of air-filtering plants
that can eliminate significant amounts of formaldehyde, xylene, benzene,
etc. (Source:   http://en.wikipedia.org/wiki/List_of_air-filtering_plants).

Lucie
UCSD
Dept. of Pathology


On Friday, May 11, 2012, Victoria Baker wrote:

 It's probably more toxic for the plants, but I like having them and no one
 has told me I had to remove them.  Ivy's are the most sturdy and the green
 color just perks up things.
 On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab sohra...@ah.org wrote:

 
  I was told by infectious control person that plants are not allowed in
 the
  lab?? IS this true? any experience with this?
  Thank you, Behnaz
 
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RE: [Histonet] Re: Redneck Lent

[Susan.Walzer]

I'm Frisbeetarian, we believe when you die your soul goes up on the roof and 
you can't get it down.  :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood 
(SCHN)
Sent: Tuesday, March 27, 2012 7:28 PM
To: 'Bob Richmond'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Redneck Lent

Roman or Anglican or Episcopalian?

Well I'm Australian!!


Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Wednesday, 28 March 2012 8:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Redneck Lent

I think this thread is the longest I've seen in fifteen years on Histonet! I 
find the joke a little lame, but the thread gets funnier and funnier.

If the joke gives offense, it's because its writer clearly has only a very dim 
idea of Catholic practice (either Roman or Anglican - I'm an Episcopalian). It 
would be lot funnier if these details were rewritten.

Lesley Weston then tried to tell a comparable story from Jewish history, only 
to have it intercepted by his corporate netnanny, apparently because it 
contained the word J-w. I think of playwright Herman Wouk's book about his 
Orthodox faith he published about 50 years ago - This Is My God is I think 
the most profound work of religious apologetic written in the 20th century - 
well, maybe C.S.
Lewis's Mere Christianity - Wouk the playwright wryly observed that the word 
J-w uttered on the stage is always a shocker.

As an aside, when you forward anything on the Web, make sure always to delete 
all the accumulated e-mail addresses in it - they're an invitation to spammers.

Bob Richmond
Samurai Pathologist
Knoxville TN

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RE: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY

[Susan.Walzer]

Overnight processing is usually optimal for average size tissue. Larger tissue 
may not get optimal processing and biopsies may be a little over processed. It 
is the nature of tissue processing in a general lab that does not have several 
processors with different programs.  A solution is to rehydrate after facing in 
the block. I keep soapy water( any liquid soap will work) to which I have added 
some ammonium hydroxide and pour it on my ice. ( ammonia is great for bloody 
tissue) for soaking. Remember what Lee Luna said, rehydrate, rehydrate, 
rehydrate.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wilson A
Sent: Friday, February 24, 2012 10:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY

 
    Hi,
   Please we are having problems getting great slides from tissue/biopsy 
specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our 
pathologists are not happy at all because of this situation.
   I will really, really appreciate if  you guys in histoland could suggest 
some solutions that could stop the problem.
   Hoping to read from you guys asap. You guys are the best.
 
Thanks,
 
Wilson
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[Histonet] RE: Opinions on Leica stainer

[Susan.Walzer]

Workhorse...has all the requirements we need.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Giracello, 
Darlene
Sent: Thursday, February 16, 2012 12:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Opinions on Leica stainer


We would appreciate any opinions on the Leica Autostainer XL ST5010 and the 
ST5020.
Any likes, dislikes, has it been reliable? Consumables? Service?
We are a veterinary diagnostic lab, so flexibility is very important for us.

I bow to the wisdom of Histonet!!!

Thanks
Darlene
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RE: [Histonet] RE: time off

[Susan.Walzer]

Time off is one thing but holidays are another. Holidays need to be rotated. 
Seniority should apply to things like choice of hours but not holidays.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting
Sent: Wednesday, January 04, 2012 8:47 AM
To: Andrea; Toni Rathborne
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: time off

I've witnessed that granting time by seniority lets you open to abuse. I know 
of an employee with high seniority who takes the whole week after Christmas 
every year. Our policy allows only one person off per day per shift. So then no 
one else ever can take off at Christmastime. Her peers complain to management 
about it, but they won't say anything to her.  
Just my two cents.
 
 
 Andrea abeharry...@gmail.com 1/3/2012 5:40 PM 
We also have a policy on the number of people that can be off during peak 
times. Our vacation schedule runs from June to June of the following year. 
Staff have up until a deadline of January 31 to put in their request for the 
next vacation year, whether it is one day or weeks. The requests handed in by 
this time period are granted based on seniority. ( in our institution they 
figure it's one of the only perks to being a senior!) After the January 31 
deadline it is first come first serve. All vacation requests must be submitted 
on a vacation request form and time stamped when handed in. This way if two 
people ask for the same day the person who handed it in with an earlier time 
stamp is granted the time off.
All of this is written in our scheduling guidelines. It seems to work pretty 
well.



On 2012-01-03, at 4:32 PM, Rathborne, Toni 
trathbo...@somerset-healthcare.com wrote:

 We have a Laboratory policy which states that holidays will be rotated. There 
 is also a section which gives a limit to the amount of time an employee can 
 have off during peak vacation time. For example, our staff can only have a 
 maximum of two weeks off during the peak summer time, and no more that 2 days 
 off during the last two weeks of December. I personally have no problem with 
 staff requesting time off early in the year, but I do ask that they discuss 
 with their coworkers before giving me the request. 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
 Sent: Tuesday, January 03, 2012 4:10 PM
 To: histonet@lists.utsouthwestern.edu 
 Subject: [Histonet] time off
 
 
 Those of you who are supervisors, how do you handle your co-workers asking 
 for time off?  I have 2 employees that have asked off already (jan 3rd) for 
 every day they want off for the entire year!  Do you grant them the days off 
 since no one else has asked off yet, or tell them it's not fair to 
 continuously get off around every holiday by asking off  5 - 12 months in 
 advance? 
 
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[Histonet] RE: time off

[Susan.Walzer]

We usually go by first asked days off. (We keep a calendar for this purpose) 
But for holidays we rotate. If someone had the day after Thanksgiving off last 
year they let the next in line have the choice.( I keep the previous years 
calendars for checking) If no one else wants it they can then have it. It has 
worked out pretty well over the years.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Tuesday, January 03, 2012 4:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] time off


Those of you who are supervisors, how do you handle your co-workers asking for 
time off?  I have 2 employees that have asked off already (jan 3rd) for every 
day they want off for the entire year!  Do you grant them the days off since no 
one else has asked off yet, or tell them it's not fair to continuously get off 
around every holiday by asking off  5 - 12 months in advance? 

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[Histonet] RE: Tissue Loss

[Susan.Walzer]

When tissue falls off the slide it is usually poorly cut sections or a problem 
with under processing or bad tissue ( bones) GI biopsies should not be falling 
off if cut well and processed correctly. Keep an eye on how she is cutting, she 
may have a problem with her microtome ( are all screws tight and is the angle 
right?) Is she cutting too thick? Is she ribboning? If a tech cannot get a 
ribbon on a biopsy she is doing something wrong.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith
Sent: Monday, December 05, 2011 9:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Loss

I would like some input on a situation I am faced with in our lab. We
are constantly having tissue coming off the slide on GI bx's. This is
coming from one tech, she is a senior tech and takes offense to
questioning her about the problem. From what I can tell all of the techs
are following the same protocal.
 
Judith Gale Pardue HT(ASCP), QIHC
Histology Supervisor
423-495-5756
judith_par...@memorial.org
Memorial Healthcare System
 
To the world I'm one, to one I'm the world
 

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RE: [Histonet] Xylene sensitivity

[Susan.Walzer]

If you use Clearium mounting media from Surgipath you can coverslip out of 
isopropyl alcohol.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Tuesday, September 27, 2011 11:35 PM
To: D'Attilio, Shelley
Cc: Histonet Listserv (E-mail); histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene sensitivity

I understand the reluctance to discontinue the use of the xylene in the 
tissue processor for FDA compliance, but why not change the solvent for 
staining and coverslipping?  Just curious.
Jennifer MacDonald





D'Attilio, Shelley sdatt...@stormontvail.org 
Sent by: histonet-boun...@lists.utsouthwestern.edu
09/27/2011 01:25 PM

To
Sarah Holmes sa...@kidneybiopsy.com
cc
Histonet Listserv \(E-mail\) histonet@lists.utsouthwestern.edu
Subject
RE: [Histonet] Xylene sensitivity






Hi Sarah,
Thanks so much for the information.  We do have a small fume extractor 
next to our formalin containers in the Gross Room.  This might be worth a 
try for the coverslipper anyway.  I believe that the piece of equipment I 
am thinking of buying while we evaluate xylene replacements is a larger 
version of that type of device, so it might work quite well.

Thanks for taking the time to respond,
Shelley

-Original Message-
From: Sarah Holmes [mailto:sa...@kidneybiopsy.com]
Sent: Tuesday, September 27, 2011 12:22 PM
To: D'Attilio, Shelley
Subject: Re: [Histonet] Xylene sensitivity


Not fully understanding your setup, we have had great success with 
inexpensive local fume extractors, like welders use, at our grossing and 
coverslipping sites.  We still hand coverslip in front of a container of 
xylene (holding roughly 400ml) and we set the fume extractor right behind 
it 
to divert all fumes thru the charcoal filter.  The fan competes so 
strongly 
with airflow that xylene (formalin) fumes are never in the breathing zone 
of 
the worker, and they are run thru an activated charcoal filter which we 
change every 3 months.

Available at technitool.com, item number 272SO350 is the extractor, 
272SO352 
are filters.

Hope this helps!

Sarah Holmes
Laboratory Manager
Laboratory for Kidney Pathology, Inc.
1916 Patterson St, Suite 501
Nashville, TN 37203
615-321-5729






- Original Message - 
From: D'Attilio, Shelley sdatt...@stormontvail.org
To: Histonet Listserv (E-mail) histonet@lists.utsouthwestern.edu
Sent: Tuesday, September 27, 2011 8:27 AM
Subject: [Histonet] Xylene sensitivity


Hi all,
I have a new employee who is developing a scratchy, painful throat and 
some 
difficulty breathing when exposed to xylene (for instance, when the cover 
is 
raised on the coverslipper).  This is her first job in a lab of any sort. 
We are investigating all the usual culprits--air handling system, hoods, 
allergies or virus unrelated to histology, etc.  Right now she is wearing 
a 
PAPR to work, which is obviously not a long-term solution.  Ultimately, I 
think we will conclude that this employee has a sensitivity to xylene and 
possibly other chemicals in the histology lab, as other employees are not 
complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?  I 
am 
looking at a portable unit that sits on casters and provides 4 air 
exchanges 
per hour.  It's not cheap at $1000, but well worth it if it will provide 
relief for this employee and allow her to continue her employment.  Our 
lab 
is approximately 800-1000 sq. ft in size with 8 foot ceilings.  We have 1 
standard bio-hood for processing cytology fluids and 2 wall-mounted air 
suckers above our processors.  I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




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[Histonet] RE: Assisting with Autopsies

[Susan.Walzer]

We are a small hospital and though we no longer do in house autopsies we still 
had an autopsy assistant.(most prefer to be called this) We always had a pool 
of people available to do this job. Training as a histotech does not include 
this job and I have always refused to do it. I know there are techs who do not 
mind and some who supplement their income doing it but histotechs should not be 
forced to do them, certainly not free of charge. If enough histotechs in an 
area stand together and refuse the pathologists will find assistants.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self
Sent: Tuesday, July 19, 2011 3:12 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Assisting with Autopsies 

Hello All,

We are small hospital that does approximately 5-10 autopsies a year.  This 
being said our administration department does not want to hire a diener to 
assist with these autopsies. So I have decided to turn to all of you out there 
in histoland for a little poll.

Does your facility use histotechs or a diener to assist with the autopsy?


Thanks in advance for all of your help, Amy


Amy Self
Georgetown Hospital System
843-527-7179
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RE: [Histonet] How many tissues an histo tech is suppose to cut per

[Susan.Walzer]

Would you ask a surgeon to speed up his procedure? We are also using sharp 
blades and cutting off the tip of a finger is a real outcome to speed cutting. 
What we do effects patient outcome and quality must always come first. Speed 
come gradually after. When you treat patient tissue on a assembly line you 
diminish the importance of what we do. The pathologist cannot diagnose disease 
without quality slides.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
sgoe...@mirnarx.com
Sent: Monday, June 27, 2011 1:52 PM
To: lbla...@digestivespecialists.com; joanne0...@comcast.net; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per

I second this motion!!

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Monday, June 27, 2011 12:10 PM
To: 'Joanne'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut
per

Unless you are in some remote area that there aren't any other
facilities around, I would look for a new job!  I don't think your age
should have any bearing on finding one.  If you were close to me I'd
hire you.  Working under that kind of condition is unacceptable in my
opinion.  It promotes errors and that isn't what we are all about.
Those blocks are our patients.

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne
Sent: Saturday, June 25, 2011 2:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] How many tissues an histo tech is suppose to cut per


 
i've only been working 2 months.  although older, i am new as a
histotech (graduated in may 2010, found a job in april 2011).  seems
management is setting a goal of a block per minute as far as cutting
goes for me.  i have until october to attain this goal. this minute for
cutting is to include facing, writing out slides, cutting, and putting
tray into symphony stainer (not to mention getting up to answer the
phone, fielding questions regarding send-out cases, and other slight
cutting interruptions).   this seems an extreme, possibly unattainable
goal.  i'm up for a challenge  at age 53, but any advice would be
SWONDERFUL :)   
 
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RE: [Histonet] PAS with Diastase Digestion

[Susan.Walzer]

Not spit ,saliva and we leave it on for 15/20 minutes and then rinse well. I 
have had techs who did not like doing it so we bought the enzyme and after all 
the hassle she found it did not work as well...now we all think about chocolate 
and salivate. :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks
Sent: Wednesday, February 09, 2011 7:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS with Diastase Digestion

Hi Histonet-



I recently ran a PAS/D stain and had some issues with it.  Both with and
without slides came out looking the same so I'm guessing my digestion step
didn't work!  I used a Malt Diastase solution (0.5g to 500mL water) for my
digestion.  This is the procedure I used:



1.Deparaffinize and hydrate to water.

2.Place the sections labeled “with” in diastase solution preheated to
37˚C for 1 hour.  Hold the sections labeled “without” in distilled water.

3.Wash in running water for 5 minutes

4.Place all section (with and without) in 0.5% periodic acid solution
for 5 minutes

5.Wash in 3 changes of distilled water

6.Place in Schiff reagent for 15 minutes

7.Wash in lukewarm tap water for 5 minutes (immediately sections turn
dark pink color).

8.Counterstain in Mayer’s Hematoxylin for 3 minutes.

9.Wash in tap water for 10 minutes

10.   Dehydrate starting with 95% ETOH, clear, and coverslip.



I am wondering if my solution possibly got too warm in the oven and hindered
the enzyme activity, or is it possible I left it in too long?  Any tips
would be much appreciated!  Oh, and I have about 300 slides to stain, so
spitting on them is my last last last resort!  Haha!  Thanks in advance for
all your help!







Erin Sarricks, HT (ASCP)

Histology Laboratory Technician

USAMRICD Comparative Pathology Branch

Office: Bldg E-3081 Room 178

E-mail: erin.p.sarri...@us.army.mil
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[Histonet] RE: Use of ammonia water

[Susan.Walzer]

We have always used water with liquid soap and some ammonia to soften blocks. 
It has had no effect on IHC.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
Sharon.Davis-Devine
Sent: Monday, December 27, 2010 3:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Use of ammonia water

Happy Holidays Histonetters, I have a question for you. Do any of you use 
ammonia water to soften the blocks before cutting? If so, does this have any 
effect  on IHC staining? Any help will be greatly appreciated. Thanks.

Sharon Davis-Devine, CT (ASCP)
Cytology-Histology  Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinois 61801
217-383-3572
sharon.davis-dev...@carle.com

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RE: [Histonet] Eosin to dye small Biopsies

[Susan.Walzer]

We use about the same amount but in our last 100% Alc.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histot...@imagesbyhopper.com
Sent: Thursday, October 21, 2010 4:40 PM
To: Scott, Allison D
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin to dye small Biopsies

We use about 40ml of eosin in the first 100% alcohol in both of our large 
specimen  small biopsy machines.

Michelle



On Oct 21, 2010, at 4:33 PM, Scott, Allison D allison_sc...@hchd.tmc.edu 
wrote:

 Hello to all in histoland.  Are any of you using eosin on the processor
 to dye your small bx's?  If so, are you putting it in the 100% alcohol
 to do so?  Any help in this matter will be greatly appreciated.
 
 
 Allison Scott HT(ASCP)
 Histology Supervisor
 LBJ Hospital
 Houston, Texas 77026
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[Histonet] RE: Plain Vanilla Autostainer?

[Susan.Walzer]

Leica Auto Stainer is the only way to go. It does all that you ask for and ours 
is old and still a work horse.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin
Sent: Tuesday, October 19, 2010 2:50 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Plain Vanilla Autostainer?

I am a simple man in a simple lab with simple needs.  I work in a multiple-lab 
research environment, typically providing paraffin/frozen sections + HE to 
individuals who then perform their own manual IHC.  A simple man, yes, but also 
a busy man, who needs to be freed from staining dishes in order to pursue other 
tasks.

What we need: (1) Deparaffinization only; (2) Deparaffinization -- HE; (3) 
HE only system, preferably with (4) a relatively small, preferably benchtop 
footprint (current unit lives in a 30 X 33 X 34 vented workstation).  
Automatic coverslipping extremely optional.

What we have: Thermo Shandon Varistain 24-4, which performed these mundane 
tasks admirably until going electronically insane.  It's been denied a service 
contract, the production line has been discontinued and the writing is on the 
wall.

What seem to be available:  Special staining systems with a whole lot of bells 
and whistles, designed primarily for a high-throughput hospital setting.

Is there anything out there that would serve my needs, even if it is a fancy 
system that would be slumming it here?  Or is mine just too obsolete a market?  
Price up to $15K, slightly negotiable.  (Bench space only slightly negotiable; 
floor model if I must.)

Many thanks,

Kevin Johnson
University of Miami
Diabetes Research Institute
Miami, FL
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[Histonet] RE: Cleaning molds

[Susan.Walzer]

Ours go on the VIP during the cleaning cycle. Then we dip them in a bucket of 
alcohol containing mold release and spread them out to dry on a clean towel.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L
Sent: Thursday, August 26, 2010 11:54 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Cleaning molds

Does anyone in histoland clean their embedding molds in a dishwasher?  
Otherwise, besides placing in the cleaning cycle of your processor, how do 
sites clean their molds??  Simple, but plaguing question!!!  Thanks all!

Dorothy Webb



  
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RE: [Histonet] assisting with patient bone marrows

[Susan.Walzer]

Everyplace I have worked it was done by hematology. When hemo tried to dump 
this job on us the pathologist stepped in to remind them that this is their 
area of expertise. We do not expect hemo to do frozen sections and we do not do 
bone marrows. After they have made their smears and sent the flow they drop off 
the core and asp. and one smear for iron to us. We handle decaling the bone.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
wanda.sm...@hcahealthcare.com
Sent: Wednesday, July 14, 2010 8:29 AM
To: lynnlee2...@live.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] assisting with patient bone marrows

Good Morning,
At this point, our Hematology Dept assists on BMs on the floors.  If they 
could, they would pass it on to us, but we do not have the staff (or desire) to 
do it.  My past 2 HT hires came from places where the histotechs assisted on 
the BM collection.
Hope that helps!
Wanda 


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynn L
Sent: Tuesday, July 13, 2010 9:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] assisting with patient bone marrows


Can anyone provide some input about which department  (Hematology, Histology 
etc.) assists on the patient floors with bone marrow aspirations and to what 
extent? Thanks in advance!

 

Lynn Lee

Casa Grande, AZ

 
  
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RE: [Histonet] Help! In need of positive Gram Control

[Susan.Walzer]

Take some fresh tissue (we use umbilical cord)  to micro and let them incubate 
in for a few days in gram + and/or negative. Then fix it and you will have good 
controls.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of connie grubaugh
Sent: Tuesday, June 22, 2010 9:06 PM
To: cg...@marylandgeneral.org; jcbrit...@cheshire-med.com; dianar...@aol.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help! In need of positive Gram Control


Tried the slim jim and all of my doctors did not like it.  Don't waste your 
time.



Connie G.



 

 Date: Tue, 22 Jun 2010 10:16:14 -0400
 From: cg...@marylandgeneral.org
 To: jcbrit...@cheshire-med.com; dianar...@aol.com; 
 histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Help! In need of positive Gram Control
 CC: 
 
 You have got to be kidding!! That's hysterical. So process a slim jim
 and you have 
 Gram - and + controls. If you're serious I'm trying it. 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Josie
 Britton
 Sent: Tuesday, June 22, 2010 6:10 AM
 To: dianar...@aol.com; histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Help! In need of positive Gram Control
 
 
 
 
 
 Have you tried a Slim Jim? They have gram positive and negative rods in
 
 them. Regardless, I still enjoy eating them once and a while! 
 
 
 
 
 
 
 
 Josie Britton Ht
 
 
 
 Cheshire Medical Center
 
 
 
 Keene, NH 03431
 
 
 
 
 
 
 
 
 
 
 
 -Original Message-
 
 From: histonet-boun...@lists.utsouthwestern.edu
 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
 
 dianar...@aol.com
 
 Sent: Monday, June 21, 2010 7:43 PM
 
 To: histonet@lists.utsouthwestern.edu
 
 Subject: [Histonet] Help! In need of positive Gram Control
 
 
 
 
 
 
 
 Help! We are in need of positive Gram Control Blocks if anyone has any 
 
 
 
 extra they are willing to part with. I have lots of Fungus,
 
 Pneumocystis and 
 
 
 
 HPV tissue blocks to trade.
 
 
 
 
 
 
 
 Diana Ripley
 
 
 
 John Muir Histology
 
 
 
 Concord Campus
 
 
 
 2540 East Street
 
 
 
 Concord, CA 94520
 
 
 
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 Histonet mailing list
 
 
 
 Histonet@lists.utsouthwestern.edu
 
 
 
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 
 
 
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RE: [Histonet] Masson Trichome Staining: Can I use Fast Green?

[Susan.Walzer]

We use 5 minutes on FG.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cscam...@uci.edu
Sent: Thursday, April 08, 2010 6:42 PM
To: HistoNet
Subject: [Histonet] Masson Trichome Staining: Can I use Fast Green?

Hi Histonet,

I am currently using the protocol from Stainsfile for staining heart
tissue: http://stainsfile.info/StainsFile/stain/conektv/masson.htm

My lab had Fast Green readily available so I substituted it for Light
Green in Solution C. The slides have not been coming out well. I failed to
account for the difference in timing between FG and LG - running the slide
in FG for the full 10 minutes. Has anyone used FG in Masson's Trichrome?
If so, how long did it take to get the desired colors in the solution? Or,
is Fast Green a poor substitute for Light Green?

Thanks for your advice!
-Colin


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