Re: [Histonet] Advice for new grads?

[Victoria Baker via Histonet]

Pam,

So far a lot of excellent advice has been given. Along the lines of what
Tim has said, learn new tests. Any chance to get scope time with a
pathologist one on one or in a multi scope setting is an excellent learning
tool. It shows your interest & you will get to know your pathologists. This
will also encourage them to feel more comfortable in the lab.
Try different lab settings. Academic research you have a lot of interaction
with scientific people & you will have to figure things out/research them
yourself.  Clinical is where most people start out & what Tim said is spot
on. Pharma is really incredible, especially now. It will most likely mean
working with animal, cell culture & archival materials. You’ll need to
bring your A game to work each day, but most of the people in all these
settings will be willing to show an eager learner.
Good days come with bad days. It’s hard to not get discouraged, but
remember it is just one day.
Go get ‘em!

On Mon, May 9, 2022 at 1:16 PM Pam Barker RELIA Solutions via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi Histopeeps!
>
> I hope this is the beginning of a fantastic week for you.  On Wednesday I
> will be speaking to a group of newly graduated histotechs.
>
> I wanted to ask if you could give someone just entering the field of
> histology a single piece of advice what would it be?
>
>
>
> Thanks-Pam
>
>
>
> Right Time, Right Place, Right Move with RELIA!
>
> Providing excellent service exclusively to the Histology Community!
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>
>
> Thank You!
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>
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> President/Senior Recruiting Specialist-Histology
> RELIA Solutions
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>  
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Re: [Histonet] autopsy blocks

[Victoria Baker via Histonet]

Hi!

We are required to keep all of our materials for 20 years.

CAP I think is less, but we have to follow NYS.

Vikki Baker

On Fri, Jun 4, 2021 at 1:22 PM Nancy Schmitt via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello-
>
> Can you tell me what you are doing with autopsy blocks?
> How long are keeping these blocks/slides?
> Appreciated,
> Nancy
> Dubuque, IA
>
>
>
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Re: [Histonet] Question - Bone marrow core biopy specimens

[Victoria Baker via Histonet]

Hi Richard!

That is the duplicate of what we do in our lab.  We were putting both the
core and the clot on the same slide for in house testing, but den outs we
sometimes have to split the specimen and only on a slide.

I have a question for you about ISH Kappa/Lambda.  What decal protocol do
you use and do you see edge non specific detection/substrate on your
slides?  We use the Roche Iview blue.

I hope what I said helps.

Vikki

On Mon, May 3, 2021 at 4:12 PM Cartun, Richard via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello everyone:
>
> I'm wonder what your protocol is for cutting formalin-fixed,
> paraffin-embedded bone marrow core biopsy specimens?  Do you cut a limited
> number of slides upfront and then go back to the block if the pathologist
> requests histochemical stains or immunohistochemical tests?  We are
> implementing a protocol today where we will cut "15" slides upfront; 3 H
> at different levels and 12 unstains for potential histochemical stains
> and/or IHC tests.  Are we crazy?
>
> Thank you, and I hope everyone is doing well.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic
> Proteomics Laboratory
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 972-1596 (Office)
> (860) 545-2204 (Fax)
> richard.car...@hhchealth.org
>
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[Histonet] Frozen section QA

[Victoria Baker via Histonet]

Happy Monday morning!

We recently had an incident which led us to review our policy on frozen
section QA.
In your institution does the same pathologist who did the frozen section
read the permanent sections?  Also if they do then who is the pathologist
responsible for doing the QA?  How is this work flow completed?
Last, does any facility with less than 450 beds have their pathologists
specialized.

Thank you in advance for your help.

Have a wonderful week ahead!

Vikki Baker
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Re: [Histonet] QIHC Prep

[Victoria Baker via Histonet]

Ariel,

Go to the NSH learning they have a very good tool there.

Vikki

On Mon, Sep 14, 2020, 10:29 AM Ariel Liberda via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello all!
> I hope everyone is staying safe!
>
> I'm looking to get my QIHC and I was wondering what prep materials
> everyone found most useful? I've looked at the histonet archives and
> everything I found was mostly out of date. I'm also looking for info about
> the test itself. How difficult did you find it? Someone mentioned that they
> believed it was a test taken with notes available but I haven't been able
> to find confirmation of this. Any info will be helpful! I've worked with
> IHC's for a few years now so I'm looking forward to getting the official
> accreditation!
>
> Thank you all in advance! Histonet is such a wonderful wealth of knowledge!
> -Ari
>
> --
>
> Ariel Liberda, HT(ASCP)
> Lead Histotech
>
> CTALab
> c. [503.906.7300](
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Re: [Histonet] question about Digital Pathology Certificate

[Victoria Baker via Histonet]

Ranna,

I took the course and it was well worth the cost for me.  As to how it is
assisting my career right now it isn't as my facility stopped using imaging
about a year and a half ago.  With the pandemic they may be rethinking
this, but it won't be immediate.
This type of skill will be more important, I believe, with the advancement
of telemedicine and what we should be learning from the COVID-19 shut down
or limited resources of certain services.  Plus with so many facilities
merging to survive in the current environment this is going to be necessary.

What I would like to see is more follow up courses and more attention drawn
to this program.

Vikki



On Mon, Apr 6, 2020, 7:48 PM Ranna Mehta via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi All,
> I need inputs from my histo colleagues about Digital Pathology
> certification from NSH. If anyone has done this program, how is it helping
> in their career?
> what are pros and cons? Do they include MatLab  and R programming in
> syllabus? I know it is very resourceful in research lab but what about
> Clinical labs?
> Right now i am working on multiplex staining using Vectra inform and Halo.
> I am self learner; I don't have any help except company's technical
> support. I am wondering- attending this program will help me understanding
> algorithm and analysis? Thanks in advanced. Stay safe.
> sorry for lots of questions.
> Regards
> Ranna Mehta
> Associate Scientist
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Re: [Histonet] CPT Code 88172

[Victoria Baker via Histonet]

Hi,

I just referred back to my sometimes frustrating clear as mud 2019 CPT
coding binder.
88172 is not saying that it has to be done by a pathologist.  In many cases
it is completed by a Cytotechnologist.
The precise wording says
"Cytopathology, evaluation of fine needle aspirate; immediate
cytohistologic study to determine adequacy for diagnosis, first evaluation
episode each site
The professional code comes in on 88173 where it involves the
interpretation and report.

The stance that it is a tech component comes from the
Cytologist/pathologist obtaining the specimen.  However they are
responsible for the adequacy of of the specimen.  The argument goes both
ways.

At this institution we use it as a professional code only.

Vikki

On Wed, Sep 4, 2019, 11:43 AM Knutson, Deanne via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello Histonetters!
>
> I am wondering how others may be using the cpt code 88172-T?  Like in what
> type of scenarios?
>
> Thank you for your responses.
>
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknut...@primecare.org
>
>
>
>
>
>
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[Histonet] Roche Symphony and Ultra users

[Victoria Baker via Histonet]

Happy Friday!

I'm looking to see if anyone out there is using these two specific
instruments together.

For about 8 or 9 months we have been experiencing what looks like water or
water droplets on the IHC slides.  It's not on all slides and we can go a
span of time with no issues only to have it come back.  It's not antibody
specific either.  We wash (Dawn dish detergent) and then load into the
Symphony.

I know there is the human factor, but right now I am just looking to see if
anyone is or has experienced this.

Thanks and enjoy your weekend.

Vikki
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Re: [Histonet] CD27

[Victoria Baker via Histonet]

On Mon, Jul 29, 2019, 9:45 PM Victoria Baker  wrote:

> Hi!
>
> Is anyone currently using CD27 on FFPE human tissue?
>
> I found one source and it is for lyophilized CD27.  I'd like to find one
> that is a concentrate.  I have given up on a pre-dilute.  I would like to
> see if there is both a mono and a poly clonal ab available and is I'm use.
>
> Thank you in advance for your help.
>
> Vikki
>
> Thank
>
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[Histonet] CD27

[Victoria Baker via Histonet]

Hi!

Is anyone currently using CD27 on FFPE human tissue?

I found one source and it is for lyophilized CD27.  I'd like to find one
that is a concentrate.  I have given up on a pre-dilute.  I would like to
see if there is both a mono and a poly clonal ab available and is I'm use.

Thank you in advance for your help.

Vikki

Thank
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Re: [Histonet] Use of Freeze Spray in a Cryostat

[Victoria Baker via Histonet]

Jennifer,

I called Leica as we have 2 cryostats at different locations so we needed
to be sure that we could use our current spray that we get from Leica.

We were told that their spray with the 1,1,1,2 Tetrafluoroethane is safe to
use.
I would therefore check to see what you have in stock and if it is this you
should follow the guidelines on the can.

We do not use spray on possible airborne pathogen cases and we avoid
spraying the specimen directly at all times.

Hope this helps.

Vikki


On Tue, May 28, 2019, 1:11 PM Schumacher, Jennifer J via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi all,
> Looking for some documentation regarding the use of Freeze Spray in
> cryostats.
>
> A Best Practice document would be ideal, but in the absence of such a
> document, I would like to hear how others are planning to address the
> "recall" by Leica where they do not recommend the use of the Freeze Spray
> in their cryostats due to the possibility of a fire (flammable reagent).
>
> I personally have always had concerns around infection since the specimens
> are fresh and using the spray could aerosolize any infectious agents that
> are present.
>
> There is a lot of pressure to freeze things rapidly to meet the FS TAT, so
> the battle goes back and forth.
>
> Any feedback is much appreciated.
>
> Jennifer
>
>
> 
>
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[Histonet] ISH kappa lambda on bone marrow core biopsy

[Victoria Baker via Histonet]

Good morning!

Just recently we started having an issue with a heavy blue precipitate on
our bone marrow cores.  We have changed nothing in our protocol and even
did parallel testing with a reference lab.  Both had the precipitate, but
to a lesser intensity.

Has anyone else been seeing this?

Thank you in advance any and responses are appreciated.

Vikki
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Re: [Histonet] Air pocket in paraffin blocks

[Victoria Baker via Histonet]

Thank you everyone for your responses.  It is very much appreciated.

It is an up hill issue as most of the technical staff have been here and
maybe one other lab in their entire career and "no one's complained
before".  I'd like that phrase and "that's the way we've always done it"
stricken from all languages and any alien dialects we might ever encounter
in our lives.

Happy Friday and have a great weekend!

Vikki

On Fri, Mar 8, 2019, 2:38 PM Mayer,Toysha N via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Vikki,
>
> I teach students that they should not have any air bubbles in their
> blocks.  This means those under the lip of the cassette as well as
> surrounding the tissues.  Air bubbles can cause instability in the block
> especially if the tissue is hard or very small.  When they go to a clinical
> rotation no preceptor checks their blocks as closely as I do, but they can
> get a 'perfect' block without much effort.  Our tissues and cassettes have
> mostly been donated, so the quality may not be as optimal as that in a
> clinical lab, so if they can get a good block embedded with me, they can do
> it anywhere.
> It is actually a scored item on our graded checklist, along with the
> overall embedded time.
> You might want to do an in-service on air bubbles and give them a CEU for
> it.
>
> Hope this helps.
>
> Toysha N. Mayer D.H.Sc., MBA, HT(ASCP)
> Assistant Professor/Associate Program Director
> HTL Program
> MD Anderson School of Health Professions
> 713.563.3481
> tnma...@mdanderson.org
>
>
>
> Message: 1
> Date: Fri, 22 Feb 2019 18:32:41 +
> From: "deGuzman, Jose R" 
> To: Victoria Baker ,
> "histonet@lists.utsouthwestern.edu"
> 
> Subject:
> Message-ID:
> <
> by2pr13mb06966aaed227c932c333509196...@by2pr13mb0696.namprd13.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="utf-8"
>
> Hello Vikki,
>
> What you are experiencing is the lack of quality feedback. The
> "experienced" techs may have never been told that their quality needs to
> improve due to complacency or management is afraid they might leave. This
> gets worse if they have gotten away with producing poor quality for a long
> time and nobody has provided much needed feedback to them. You may have an
> uphill battle ahead of you but very much worth taking.
>
> Jose
>
> -Original Message-
> From: Victoria Baker 
> Sent: Thursday, February 21, 2019 5:49 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Air pocket in paraffin blocks
>
> Hi,
>
> When I was trained to do embedding there were many things that the
> professor stressed to me need to be done in order to have the tissue block
> acceptable for sectioning.  One of these was air bubbles.
>
> Recently we had a new tech embed a derm block that had an bubble that was
> pretty big.  The other (experienced) techs didn't think anything of it
> either and sectioned it.  When I got the block for IHC screening I made a
> QA form stating that the block should have been re-embedded before giving
> it to be sectioned, or when the first tech sectioned it could have repaired
> the block or melted it down.  This air bubble was big enough to be seen so
> I don't think it could have been missed - unless the block wasn't checked
> right after embedding.
>
> What has me a little upset is that no one seemed to care about this.
>
> I would really appreciate some feedback about this from other people.
>
> Thanks in advance.
>
> Vikki
>
>
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Re: [Histonet] Roche "bashing"

[Victoria Baker via Histonet]

Kelly,

It is not bashing, it is frustration and concern.

We need our instruments and reagents to work so that we deliver reliable
and consistent results to our pathologists.  They trust our work and
dedication to them and ultimately the patient.  When something like this
happens and we can't get a real resolution it undermines many things, but
trust is first for me.

Vikki

On Tue, Mar 5, 2019, 10:44 AM Terri Braud via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Such a wonderful insight into the thought process of Roche.  Accused of
> bashing when I am telling the truth about failed lots when they claim the
> problem is fixed.
> If only they would put that much effort into repairing the problem and
> fostering better customer relations, than how to market their product
> around this annoying truth.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Today's Topics:
>
>1. Re: Histonet Digest, Vol 184, Issue 1 (Jordan, Kelley)
> Message: 1
> Date: Fri, 1 Mar 2019 13:15:58 -0500
> From: "Jordan, Kelley" 
> Content-Type: text/plain; charset="UTF-8"
> More bad press in histonet...not sure if we should pass it on to
> Marketing??
> @Will, Who as Teri Blaud at   Holy Redeemer Hospital ? Teri is always
> always bashing us.
> Kelley Jordan
> Strategic Account Manager - SC, NC, TN and KY
> A Member of the Roche Group
> Ventana Medical Systems
> Mobile:  803.504.1135
> Customer/Technical Support: 1.800.227.2155
> kelley.jor...@roche.com
> www.ventana.com
>
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Re: [Histonet] Ventana dispensers - last word

[Victoria Baker via Histonet]

Terri,

We went through hell with the Roche dispensers in late 2017 to early 2018.
The only thing positive I have to say is that the field engineer and our
FAS are great.  I have little respect for the sales staff or inside
support.

We pay top dollar for these instruments and the required reagents and all
Roche shows they are truly concerned with is the next sale

We also had a sit-down with their scientific people as well,  and here we
are again.  We haven't had the magnitude of failures this time but it is
disheartening to see that what they said they were going to fix has not
been fixed.

I was also wondering why they would send product failure notifications to
clinicians and not to the laboratories that are using the reagents.  I got
a notification from one of our oncology groups - but I never received one
in our lab.

Ventana doesn't exist and I miss that very much.  With each buy out,
merger or supposed partnership that I see with suppliers and manufacturers
things are turned more to the bottom line and in the end the patient
suffers and it's wrong.

But that's big business.

Vikki



On Mar 5, 2019 3:05 PM, "Terri Braud via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

To those of you who wrote in support of my comments, thank you sincerely.
To the Company's  credit, I did receive a call of apology and a listening
ear concerning the ongoing problems associated with the dispensers.  I
sincerely hope they understand the frustration that such a problem can
cause.
I want to state that I really do like the Benchmark Ultra, but the
instrument is only as good as the individual parts.  Based on many private
responses that I received, I think that dispenser failure is probably being
vastly underreported. I can only hope that a moment's indiscretion on their
part will lead to a more open dialogue in their company concerning a true
solution to this problem.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal



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Re: [Histonet] Air pocket in paraffin blocks

[Victoria Baker via Histonet]

Yes, it does. The air bubble went directly under the tissue.

Vikki

On Thu, Feb 21, 2019, 8:43 PM Jennifer Phinney 
wrote:

> Vikki,
> Did the air bubble affect the section quality on the slide? If not, would
> it have been worth the delay to the case in order to melt and re-embed the
> tissue?
>
> I only re-embed blocks with air bubbles if it will affect the quality of
> the slide myself.
>
>
> Jennifer
>
> -Original Message-
> From: Victoria Baker via Histonet 
> Sent: Thursday, February 21, 2019 4:49 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Air pocket in paraffin blocks
>
> Hi,
>
> When I was trained to do embedding there were many things that the
> professor stressed to me need to be done in order to have the tissue block
> acceptable for sectioning.  One of these was air bubbles.
>
> Recently we had a new tech embed a derm block that had an bubble that was
> pretty big.  The other (experienced) techs didn't think anything of it
> either and sectioned it.  When I got the block for IHC screening I made a
> QA form stating that the block should have been re-embedded before giving
> it to be sectioned, or when the first tech sectioned it could have repaired
> the block or melted it down.  This air bubble was big enough to be seen so
> I don't think it could have been missed - unless the block wasn't checked
> right after embedding.
>
> What has me a little upset is that no one seemed to care about this.
>
> I would really appreciate some feedback about this from other people.
>
> Thanks in advance.
>
> Vikki
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[Histonet] Air pocket in paraffin blocks

[Victoria Baker via Histonet]

Hi,

When I was trained to do embedding there were many things that the
professor stressed to me need to be done in order to have the tissue block
acceptable for sectioning.  One of these was air bubbles.

Recently we had a new tech embed a derm block that had an bubble that was
pretty big.  The other (experienced) techs didn't think anything of it
either and sectioned it.  When I got the block for IHC screening I made a
QA form stating that the block should have been re-embedded before giving
it to be sectioned, or when the first tech sectioned it could have repaired
the block or melted it down.  This air bubble was big enough to be seen so
I don't think it could have been missed - unless the block wasn't checked
right after embedding.

What has me a little upset is that no one seemed to care about this.

I would really appreciate some feedback about this from other people.

Thanks in advance.

Vikki
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Re: [Histonet] Closed doors in Histology

[Victoria Baker via Histonet]

Mike,

Yes that is correct.  Histology labs because of their toxic chemicals and
less than pleasant odors need to keep the doors shut for proper
ventilation.  This doesn't always happen when there is a high volume of
people going in and out.
I like it to the requirement that there be a sink (clean sink) in every
Histology lab too.  We have two sinks, 1 for special stains and the other
for tissue processor loading and formalin neutralization.  Which sink do
you think might be cleaner

Vikki Baker

On Fri, Nov 16, 2018, 11:35 AM Mike Pence via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> OK, How many of you keep your doors to your Histology Dept. closed all the
> time? We were told by a CIHQ inspector that Histology Dept must be under
> negative air flow ALL THE TIME. This was a new one for me. The standard is
> from the ASHRAE 170 table 7.1
> This is just an FYI for you all that these are the kinds of things CMS is
> looking at now days.
>
> Michael S. Pence | Supervisor of Laboratory Services
> Great River Health Systems
> 1221 S. Gear Ave. | West Burlington, IA 52655
> Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557
> mpe...@grhs.net | www.greatrivermedical.org<
> http://www.greatrivermedical.org>.
> www.Facebook.com/GreatRiverHealthSystems<
> http://www.Facebook.com/GreatRiverHealthSystems> |
> www.Twitter/GreatRiverMed
>
>
> Information in this communication, including attachments, is confidential
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[Histonet] HIF-1alpha and EBNA1

[Victoria Baker via Histonet]

To All,

I am seaching for a lab that can run both of these antibodies on human FFPE
tissue.

HIF-1 alpha is Hypoxia Inducible 1 alpha
EBNA1 is Epstein Barr Nuclear Antigen 1

If anyone performs these tests or knows of a lab that does, please contact
me.

Thank you in advance

Vikki
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Re: [Histonet] HT Position in Alaska

[Victoria Baker via Histonet]

Me as well.  It's beautiful there!

On Wed, Jun 6, 2018, 12:27 PM Colleen Forster via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> This sounds wonderful..I wish I were in a position to swoop it up!
>
> Colleen Forster HT(ASCP)QIHC
>
> On Wed, Jun 6, 2018 at 9:24 AM, Arrington, Karla A via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
> > Hello All,
> >
> > I hope everyone is enjoying the early part of summer.
> >
> > In beautiful Alaska, our hospital caters to the Alaskan Native population
> > with their entire medical needs being met.
> > Currently we have a Histology Technician position available in our
> > pathology department. This position is M-F, no weekends and a rotating
> > on-call schedule.
> > This position comes with excellent benefits and a team work environment.
> > This also offers relocation assistance for the correct candidate. Must be
> > ASCP certified or eligible.
> >
> > For immediate consideration, email or FAX a current resume to
> > kaarring...@anthc.org and it will be
> > forwarded to our hiring manager. Also visit our website at anthc.org and
> > select Careers to apply online.
> >
> > If you have questions regarding this position or how to submit an online
> > application, please call ANTHC Human Resources at (907) 729-1301 or email
> > care...@anthc.org.
> >
> >
> > Karla Arrington, HT(ASCP) HIT(AHIMA)
> > Pathology Supervisor
> > Alaska Native Medical Center
> > 4315 Diplomacy Drive
> > Anchorage, AK 99508
> > Tele: 907-729-1810
> > Fax:  907-729-1226
> >
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> >
>
>
>
> --
> Colleen Forster HT(ASCP)QIHC
> BLS Histology and IHC Laboratory
> B173 PWB  612-626-1930
>
> *If submitting histology request please also forward to Lori Holm at
> ho...@umn.edu *
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[Histonet] Epic and Cerner users, looking for feedback on these systems

[Victoria Baker via Histonet]

Happy Friday!

Our hospital is starting to look at new HIS vendors.  The two that are
being strongly looked at are Cerner and Epic.

Of course the hospital is using a consulting firm who have sort of tied our
hands in terms of following up with vendors and any after the demo
questions we are to filter through them.  We have been told though, by one
of the hospital directors that we can reach out to colleagues and questions.
Currently we have SoftPath DX.  The system is labor intensive and the
version we have is not in keeping with our needs.  But it has taken us
further than we were before in terms of being able to track cases and
monitor our workflow.  We are paired in the hospital with several different
systems such as NexGen and Sorian for financials.  There is also Oracle
database which causes some headaches as well.
I would like to get an idea of what people think that use either of these
systems.  Do they slow down work flow?
  Is it generally user friendly?
 Can you track all entries and produce accurate statistics?
 Are either of these companies strong in support before, during and after
GO LIVE?
Can you incorporate PDF files into reports or access information from the
HIS into the LIS  with little or no hand to computer combat?

These are just surface type questions, but I would truly appreciate
anyone's input.

Thank you and have a nice weekend.

Vikki Baker
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Re: [Histonet] Ann Preece Manual

[Victoria Baker via Histonet]

We currently have a student in our lab.  She took her classes through
Broome community college were the program is in its infancy.  I think she
would benefit from this book.

I can give you contact information off line and it would be a hospital
address.

Vikki Baker
UHS Wilson Hospital


On Oct 28, 2017 6:31 PM, "Victor via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> I have a copy, 1st edition of A Manual for Histologic Technicians by Ann
> Preece. On the inside cover it says Wyoming Valley Hospital Laboratory
> 1962. I don’t recall how I got it, but I never worked for the facility.
> Great for a new student or Histology program. I’ll pay shipping to whomever
> is interested.
>
> Victor
>
> Sent from Mail for Windows 10
>
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Re: [Histonet] Ann Preece Manual

[Victoria Baker via Histonet]

We currently have a student in our lab.  She took her classes through
Broome community college were the program is in its infancy.  I think she
would benefit from this book.

I can give you contact information off line and it would be a hospital
address.

Vikki Baker
UHS Wilson Hospital


On Oct 28, 2017 6:31 PM, "Victor via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> I have a copy, 1st edition of A Manual for Histologic Technicians by Ann
> Preece. On the inside cover it says Wyoming Valley Hospital Laboratory
> 1962. I don’t recall how I got it, but I never worked for the facility.
> Great for a new student or Histology program. I’ll pay shipping to whomever
> is interested.
>
> Victor
>
> Sent from Mail for Windows 10
>
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Re: [Histonet] IHC counterstain offline

[Victoria Baker via Histonet]

Curt,

I am not sure how much you will actually save if you factor in the cost of
a person hand counter staining these slides.  I will be the first to say
Ventana is over priced, but off line counter staining brings in an
additional variable and cost.  In the end is it a real savings?  It's an
individual call, but I wouldn't do it.

Vikki

On Oct 12, 2017 4:43 PM, "Curt via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> We currently use the benchmark ultra and are, in an effort to save a
> little money, looking at running out counter stain offline. Anyone have any
> suggestions or thoughts they might offer? We currently run our H with
> Richard Allen Hematoxylin 7111. I am curious if that is suitable for use in
> your experience or if you recommend a different Hematoxylin for IHC counter
> stains. How much bluing time, etc.
>
>
>
> Thanks,
>
> Curt
>
>
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Re: [Histonet] on call autopsy services

[Victoria Baker via Histonet]

Have  you looked at an ad locum pathologist?  Or a retired pathologist that
would be willing to work as a per diem?

The county coroner might even be willing to get you information or know of
a pathologist willing to come in.  But do you have a dinner or a
Histologist willing to do the assisting?  There are also funeral or
certified morticians who can do assisting.

I apologise for any misspelled words.  I haven't had my tea and my phone
thinks it's a spelling scholar.
Vikki

On Aug 17, 2017 6:53 AM, "Eck, Allison via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> Good morning Histonet,
> I am not sure if anyone can help me with this question, but I was asked to
> look into the possibility of finding a pathologist who would be willing to
> come in and perform our autopsies.  We do only few each year so we are not
> looking to hire someone full time but are exploring the option of
> performing the autopsies in house.  Can anyone point me in a direction to
> start?
>
> Thanks in advance
> Allison
>
> Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT)
> Lead Tech Histology
> Doylestown Hospital
> 595 W State St
> Doylestown, PA 18901
> 215-345-2264
> a...@dh.org
>
>
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[Histonet] For NYS laboratories

[Victoria Baker via Histonet]

I'm trying to reach out to other NYS Histology labs.  I can't really get a
real answer regarding a non-CLT doing the embedding/microtomy and staining
of just Autopsy material.  I think that they can't but the actual
documentation is not completely clear.

What are other labs doing?

Thank you in advance for any information.
Vikki
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Re: [Histonet] Stand Alone Heat Extractor

[Victoria Baker via Histonet]

ThermoFisher.  It is on there website.   I don't have the catalog number
but I just ordered one

On May 15, 2017 12:48 PM, "Amanda Reichard via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> Hi Everyone
>
> I am hoping someone can help me out here...  I am in search of stand-alone
> heat extractors for our cryostat.  I am told they are hard to come by and
> was curious if anyone knew of any companies that have them?  The one I know
> of isn't an approved vendor yet, and I am trying to avoid going through
> that process.
>
> Thanks in advance!
>
> Amanda Reichard, HTL (ASCP)cm
> Histology/Cytology Supervisor
> Licking Memorial Health Systems
> 1320 W. Main St.
> Newark, OH 43055
> (220) 564-4163
> areich...@lmhealth.org
>
>
> 
>
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> have received this in error, please advise the sender by reply e-mail and
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> guaranteed to be secure or error-free as information could be intercepted,
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Re: [Histonet] Great input on recycled reagents

[Victoria Baker via Histonet]

By using a hygrometer (did I spell that right?) you should be able to see
the purity of the alcohol.  We usually get between 97 to 98 percent.  We do
use fresh absolute alcohol always in the processor.  We start with 80, 95
all recycled.

On Feb 22, 2017 4:55 PM, "Gareth Davis via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> Thank you so much.  I will start using them on the processor, but start
> slowly.  Then I can monitor to make sure no problems occur.  I really
> appreciate all the responses.
> Thanks,
>
> --
> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm
> Yuma Gastroenterology
> Yuma, AZ 85364
> 928-248-5259
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Re: [Histonet] Recycled reagents in VIP processor

[Victoria Baker via Histonet]

We routinely recycle xylene and alcohol on a CBG recycler without issues.

We also run ER PR HER2 breast panel, ISH and routine IHC

Vikki Baker

On Feb 22, 2017 3:37 PM, "Gareth Davis via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> Hi,
> I was always told not to use recycled reagents, i.e. Alcohol and Xylene, in
> processors.  I am using a VIP 300, refurbished, and I would rather not use
> recycled reagents in it.  But, during the last CAP inspection they
> suggested I use the recycled to save money.  And now my administration
> wants to cut cost.  Just wanted to know what labs were doing.
> Thanks,
>
>
> --
> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm
> Yuma Gastroenterology
> Yuma, AZ 85364
> 928-248-5259
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[Histonet] Dialyzed iron solution

[Victoria Baker via Histonet]

Hi.

Back when I was in research we did Hale's Colloidal Iron stain.  Making the
dialyzed iron was really the big part and back then we were using a
cellulose tubing that we had from another group.  I've been trying to find
the right type but there is just too many choices.  I've also been trying
to find a company that makes it as well.
If anyone can forward some information on where I could find either of
these, I would be grateful for it.

Have a happy weekend!

Vikki
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[Histonet] Cell Blocks

[Victoria Baker via Histonet]

Hi - Happy Friday!

Is anyone on makeing cellblocks with thromboplasting/plasma on cells fixed
in sodium citrate?  These would be pleural fluids and bronch brushings and
washings.

Thanks in advance.

Vikki
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[Histonet] Thromboplastin for cell blocks

[Victoria Baker via Histonet]

Hi

I am looking for Thromboplastin to make cell blocks.  I can't remember
who we got it from years ago.

Any information is much appreciated.

Vikki

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Re: [Histonet] IHC Weekend Coverage

[Victoria Baker via Histonet]

We have Saturday lab coverage which includes IHC.
Case/slides vary between 8-15/25-85.  This includes multiplex and ISH.

Vikki
On Aug 23, 2015 10:49 AM, Cartun, Richard via Histonet 
histonet@lists.utsouthwestern.edu wrote:

 How many of you working in hospital-based pathology laboratories run IHC
 on weekends?  Thank you.

 Richard

 Richard W. Cartun, MS, PhD
 Director, Histology  Immunopathology
 Director, Biospecimen Collection Programs
 Assistant Director, Anatomic Pathology
 Hartford Hospital
 80 Seymour Street
 Hartford, CT  06102
 (860) 972-1596
 (860) 545-2204 Fax


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Re: [Histonet] Breast fixation

[Victoria Baker via Histonet]

Karen
We take ours off on Sunday.  The tissue processor is set up with 6 hours of
fixation the minimum time.  We have VIP 5's so on Friday the tissue goes on
a delay run that is set to come out of formalin at 12:30 AM Sunday
morning.  The end of the process is at 9 AM Sunday morning.  Just take the
tissue off and let it solidify.   When your next tech comes in they can put
the cassette in the embedding center to heat up again and embed it.  You
will follow your same protocol and record your times accordingly.  It might
mean having to ask someone to come in for the 5 minutes it takes to do this
but it works.  If needed you can adjust your times to fit your schedule for
someone being available to take them off.
I hope this will help.
Vikki
On Jul 29, 2015 11:33 AM, Heckford, Karen - SMMC-SF via Histonet 
histonet@lists.utsouthwestern.edu wrote:

 Good Morning,
 I have a question about breast fixation.   I am in a little bit of a
 pickle with the 6-72 hour rule for the fixation on breast tissue.   Friday
 I am getting 2 breast cases in the afternoon and both will not have the
 required minimum 6 hour formalin fixation for my per diem to cut early
 Saturday morning.   He will not be able to make it in again until Monday
 night.  The tissue will be about 3-4 hours (this includes time on the
 processor)  over the 72 hour maximum.Does anyone have any suggestions
 on what can be done?  We are a one person show here.

 Thanks,

 Karen Heckford HT ASCP CE
 Lead Histology Technician
 St. Mary's Medical Center
 450 Stanyan St.
 San Francisco, Ca. 94117
 415-668-1000 ext. 6167
 karen.heckf...@dignityhealth.org

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[Histonet] Decontamination of Ventana Ultra

[Victoria Baker]

Hi,

Recently we were told that we could no longer use our Bio-med
department for doing decontamination of our instruments and that the
techs would be given an in-service to learn how to do them.  I
remember this process as long and messy.  The Ventana rep has
explained to the staff as being approx. 5 hours and not difficult to
do.  I remember it differently so I'd like to see what other Ventana
users are doing with this process.

Here are my questions:

Are you currently using in house Bio-engineering to do your quarterly cleanings?

Do you pay Ventana to come in and do it?

Are you having technical staff do it on off hours?  Then give them
time off another day?

How extensive of a decontamination do you do?  (ex - cleaning filters,
extra DI rinses, pH testing of containers to be sure all cleaning
reagents have been removed, forced air drying of bulk containers -
etc)

Have you had problems with air pressure error codes or do you increase
the pressure to handle the increased fluid flow?

Have you ever had overflow problems that damaged the instrument?

How many instruments do you have?

These are the basic questions, but if anyone has any further
information or ideas - I would appreciate hearing them.

Thanks

Vikki

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Re: [Histonet] RE: embedding

[Victoria Baker]

I have found really hot molds more difficult to work with but it is really
an individual preference.
What has worked for me is - put just enough paraffin in base mold to come
up to rim of embedding area.  Embed tissue.  Add just a little more
paraffin to bring it up to where plastic cassette top comes into contact
with hot paraffin. Tilt mold gently to spread paraffin.  Press cassette top
down and add enough paraffin to cassette top to come up half way. Do 5 - 6
blocks and then top off with hot paraffin.
The paraffin has had a chance to harden just enough to where it won't run
out from under the cassette and remain pretty clean.
Everyone has there tricks you just need to see what works best for you.
Vikki
 On Jan 21, 2015 10:28 AM, Anne Murvosh amurv...@advancederm.net wrote:

 My first job the molds were kept room temp or cool and I didn't have to
 scrape as much. My 2nd job they were kept hot and had to scrape all the
 time.  Have fun with it and test some cold, some room temp, some hot see if
 there is a difference. The problem with too cool molds is it takes a bit
 longer to embed cause the bottom takes longer to warm up so it's a trade
 off for time. Fun experiment though!  Anne

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M.
 Sent: Wednesday, January 21, 2015 7:12 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] embedding

 Hello everyone, I was just curious if anyone had tips on how to embed
 without getting paraffin on the outside of the cassettes so I don't have to
 scrape the blocks or at least not scrape very much.  Thanks!!









 Tasha Campbell, B.S.,HTL(ASCP)

 Frederick Gastroenterology Associates

 310 W. 9th St.

 Frederick, MD 21701

 301-695-6800 ext. 144 (w)

 304-685-9307 (c)



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Re: [Histonet] RE: A sticky subject....

[Victoria Baker]

Before I start cutting I spray StaticGard on the arms of my lab coat.  I
don't know what lotion you use but a non lanolin based moisturizer helped
me (corn huskers lotion - it is glycerin based).  This works for some but
it comes down to trying different things to see what will work for you.
Vikki
On Dec 30, 2014 8:53 AM, Patrick Laurie foreig...@gmail.com wrote:

 I used to have one of those anti-static brushes, it had a small radioactive
 piece of metal that discharged the static.  It worked sometimes. Try
 putting moistened gauze, or the kimwipes used to clean your waterbath in
 your microtome wax waste tray.  That seems to help me.

 Good luck.

 Patrick Laurie(HT)ASCP QIHC

 Histology Manager

 Celligent Diagnostics, LLC

 101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

 Work: 704-970-3300  Cell: 704-266-0869

 On Tue, Dec 30, 2014 at 8:45 AM, Marcum, Pamela A pamar...@uams.edu
 wrote:

  I am not sure where you live so this may or may not apply.  I have lived
  in several placed and one I learned no matter where I am when they turn
 on
  the heat and you wear anything with polyester or that can become
  electrostatic we had issues with static.  I am not sure how to fit it as
 I
  have made sure I touch something metal to discharge the static.  It does
  not cure the problem only humidity will and being careful not to start to
  cut fully charged so to say.
 
  Pam Marcum
  UAMS
 
  -Original Message-
  From: histonet-boun...@lists.utsouthwestern.edu [mailto:
  histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie
  Sent: Tuesday, December 30, 2014 7:20 AM
  To: histonet@lists.utsouthwestern.edu
  Subject: [Histonet] A sticky subject
 
  Good morning Histo folks,
 
   I am hoping somebody can help me. Today was a horrid microtomy
  day, the static was brutal. I tried putting lotion on my hands, the
 fabric
  softener sheet, and wearing gloves but to no avail. I heard rumors
 somebody
  used to manufactor an anti-static brush but have yet to find one. Does
  anybody have any other tricks that could be shared?
 
  Cassandra Davis
  cda...@che-east.org
  302-575-8095
 
 
 
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RE: [Histonet] Flames at embedding centers

[Victoria Baker]

My very first Histo job was with Metpath in Hackensack NJ.  We had Bunsen
burners right next to a xylene bath for dirty molds.  One day a tech
accidentally had a xylene Bunsen burner collision and started a fire.  We
had another tech who was quick with head and feet who was able to
extinguish it - but there was damage.  As to smoking in the lab I do
remember.  Between the fumes and the smoke I found breathing a little
difficult.  But yes those were the good 'ol days.
Vikki
On Nov 18, 2014 5:22 PM, Marcum, Pamela A pamar...@uams.edu wrote:

 We have days when one of the histologist who has been here for years and I
 (going in 50years) start talking about the good days and everyone here is
 under 40.  They look shocked and then disbelief and then they think we are
 kidding.  They are so sure we have always known which chemicals were
 dangerous or killers and no one could possibly ever have been so careless.
 WOW!  I like to show them old equipment and ask how they think it might
 work in a lecture on tissue processing.  They have no clue about an open
 processor in small room with no ventilation and we worked in it with our
 open stains lines and flames on the counter.  Now it does sound scary!


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
 Sent: Tuesday, November 18, 2014 3:04 PM
 To: Jay Lundgren; Ludlow Patricia
 Cc: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Flames at embedding centers

 Grinning and remembering the good old days.  What's more fun is the look
 of horror on the faces of the young ones when they hear it!

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren
 Sent: Tuesday, November 18, 2014 2:42 PM
 To: Ludlow Patricia
 Cc: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Flames at embedding centers

 Ahh, the good old days of walking into the lab and lighting all the
 Bunsen burners first thing in the morning.  I didn't have any hair on my
 knuckles for years.  Embedding with a Coke on the cold plate, and a smoke
 in the ashtray next to you, anyone?  Good times.

  Now we have to use forceps warmers and change forceps between
 specimens.  If you get 3 or 4 pair of forceps, one will always be hot
 enough to use.  Also, there are embedding centers with heated forceps,
 which I love .
  Just remember to clean out the wells of the forceps warmers every day
 to prevent cross contamination.  Cotton applicator swabs work great for
 this.  And always keep a towel or gauze handy to wipe the tips of the
 forceps between specimens.  Forceps warmers unfortunately don't incinerate
 any stray tissue like a Bunsen burner did.

Sincerely,

Jay A. Lundgren, M.S., HTL (ASCP)

 
 
 
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Re: [Histonet] RE: G.I. biopsies and special stains

[Victoria Baker]

AKA - managed care.  Emphasis on managed.
On Jun 9, 2014 10:11 AM, Mike Pence mpe...@grhs.net wrote:

 You gotta love Obamacare! Where Legal meets Medical.

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M
 Sent: Monday, June 09, 2014 9:05 AM
 To: kathy.mach...@lpnt.net; histonet@lists.utsouthwestern.edu
 Subject: [Histonet] RE: G.I. biopsies and special stains

 We have just discontinued this practice after Jane Pine Wood (renowned
 Healthcare Attorney and Medical Director for Palmetto Medicare Part B
 coving NC, SC, VA, and WV) stated ordering special stains prior to review
 of the HE stain is not reasonable and necessary.   According to the
 pathologist billing company ,there is in an article they have privy to with
 wording which threatens additional action taken for providers whose
 ordering of special stains exceeds the 20th percentile of Gastric Pathology
 cases.  For this reason we are now retro ordering special stains on gastric
 biopsies.  We are also looking at IHC necessity.

 Debbie M. Boyd HT (ASCP) l Chief Histologist  l Southside Regional Medical
 Center l  200 Medical Park Blvd.  l  Petersburg, Va.  23805 l  PH
 804-765-5050 l  FAX 804-765-8852

 
 From: histonet-boun...@lists.utsouthwestern.edu [
 histonet-boun...@lists.utsouthwestern.edu] on behalf of
 kathy.mach...@lpnt.net [kathy.mach...@lpnt.net]
 Sent: Monday, June 09, 2014 9:14 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] G.I. biopsies and special stains

 I was wondering if other labs are automatically ordering special stains
 and/or IHC on stomach biopsies and esophageal biopsies?  Or do you wait
 until HE is screened?  Thanks!

 Danville Regional Medical Center
 Danville, VA
 kathy.mach...@lpnt.netmailto:kathy.mach...@lpnt.net
 434-799-3868

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[Histonet] Alpha Histotech - leaving the world of histology

[Victoria Baker]

Hi Alpha,

I've been
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[Histonet] Cap on number of antibodies that can be ordered per patient/case.

[Victoria Baker]

Hi Everyone
I was approached by an administrator today who wanted to know if a limit
(cap) existed on the number of antibodies which can be ordered on a
patient.  They currently run a minimum of two antibodies on all Esophagus
cases as part of a panel.  Now they would like to increase this by an
additional 3.  These cases are referenced based and are on cell blocks
although a few of them would be small biopsies.
I was to understand that doing a one size fits all panel was sort of iffy
unless there is an established protocol that proves the necessity for
reimbursement.  I had heard about a cap on the number but can't find the
documentation.

Any and all input is greatly appreciated.

Vikki
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[Histonet] Is the server down?

[Victoria Baker]

Haven't seen any posts over past day or so.
Vikki
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Re: [Histonet] Fwd: Plants

[Victoria Baker]

It's probably more toxic for the plants, but I like having them and no one
has told me I had to remove them.  Ivy's are the most sturdy and the green
color just perks up things.
On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab sohra...@ah.org wrote:


 I was told by infectious control person that plants are not allowed in the
 lab?? IS this true? any experience with this?
 Thank you, Behnaz

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Re: [Histonet] Xylene Substitute for Counterstain Clearing

[Victoria Baker]

I think that it is called Crystal Mount - but apply to section, allow to
harden dip slide in clearing media and coverslip.  I know that there must
be others out there as well.
Good Luck



On Fri, May 11, 2012 at 12:25 PM, Andrew Coleman andrewcoleman...@gmail.com
 wrote:

 Hi all,

 We are performing a neutral red counterstain on tissue sections
 containing colored polystyrene microspheres. The spheres are inert to
 alcohol, but are washed out when we clear with xylene to coverslip.
 The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
 chloroform and methylene chloride for what its worth.

 Is it reasonable to coverslip these slides in permanent mount without
 clearing with xylene after dehydrating the tissue? Or does anyone know
 of a substitute clearing agent with chemical properties dissimilar
 enough from xylene that might be worth trying instead?

 Thanks,

 Andrew

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Re: [Histonet] Pinning Specimen

[Victoria Baker]

Hi Karen -

Are yoru eferring to the boards that have the detachable mats?  I know they
still make them and the last one I heard of was through MOPEC.

I'm probably aging myself here, but we also used to make paraffin blocks
for large specimens and pin them flat for overnight fixation - it wasn't
pretty but it worked.

Hope this helps some.

Vikki

On Thu, Apr 19, 2012 at 8:03 AM, Heckford, Karen - SMMC-SF 
karen.heckf...@dignityhealth.org wrote:

 Does anyone know where to get specimen boards that you can pin specimens
 to and then submerse in formalin?  I ordered them a long time ago and
 cannot remember where I got them.

 Thanks,
 Karen Heckford HT ASCP CE
 Lead Histology Technician
 St. Mary's Medical Center
 450 Stanyan St.
 San Francisco, Ca. 94117
 415-668-1000 ext. 6167
 karen.heckf...@dignityhealth.org

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Re: [Histonet] CLIA requirements

[Victoria Baker]

Hi Amber,

Try contacting HR and the Compliance Officer (which may be your lab
manager) as to how this requirement is handled.  Institutions vary on this,
but most times all employee files had to be with HR.  Education, CEU's,
certifications, licenses and transcripts, along with evaluations or
disiplinary actions were all based there and nothing was to be taken out or
copied.  I've been at a few places recently where accessing of staff files
was possible through the intranet - but only certain levels of supervision
were able to actually access a part or all of them.

Hope that this helps some.

Vikki





On Tue, Mar 27, 2012 at 9:30 AM, Amber McKenzie 
amber.mcken...@gastrodocs.net wrote:


 CLIA requires me to keep a personnel file on everyone that works in the
 lab and I was wondering how you approach the staff about turning in their
 highest level of education.  Do they bring in a copy of their HS diploma?
  College diploma?  And what if they're not finished with college, do they
 only turn in their HS credit?  Plus, copies of their certifications?

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Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES

[Victoria Baker]

Hi Bernadette,

I know of several different color code set  ups.
Pink - breast tissue (usually has separate processing cycle for ER/PR)
Lt. Green - biopsy tissue (has separate processing cycle - short run)
Tan -  bone marrow (has special stains and IHC built into the protocol)
Lavender - Prostate biopsies (short run also PIN4 built into protocol)
Aqua - skin/derm specimens
Red - stat or rush specimens
Grey - urate crystal (special processing)
White - all other surgical material including breast tissue that does not
require special protocols
Blue - autopsy material

In some labs they correspond the cassettes and the slides (green cassette =
green slide).  In this context you will look at having both regular
(superfrost) and plus slides available as well.

There really isn't a set protocol it is more a way of easy identification
for processing and also which case/specimen take priority.  Faster
identification for loading of processors (breast tissue, biopsy, priority
cases) ,embedding (priority and embedding orientation), cutting (special
stains, add'l sections etc).

I'm not fond of the red cassettes because they are difficult to read if not
imprinted properly, but that color stood out best and most techs associated
it with 'move it out fast'.  I did get a chuckle when a tech once asked me
why we had lavender for prostate biopsies and I had to tell him that the
blue had already been taken ;-).  One other thing that I had consternation
about was putting fatty breast tissue from a reduction in white because of
grossing/processing issues.  I could not get assistance through the Doc's
for this so there were re-pro's until I was able to get ALL breast tissue
put on the longer processing cycle.  It didn't make me a lot of friends in
the grossing area though.

For the biopsies you may want to be sure you have matching mesh cassettes.
The lavender ones I usually always used mesh.

Look at what your specimen types will be and associate them with something
that is easily recognized by staff.  There isn't a set protocol for it
though.  Sorry and I hope this helps.

Vikki








On Fri, Mar 23, 2012 at 8:42 AM, Bernadette del Rosario 
badzros...@yahoo.com wrote:

 Good day histonetters.We are a  new university hospital and setting up
 histopatholology lab.I used to work with white biopsy cassettes only but
 not technicolors.Got this boss who ask me protocols on colored cassettes
 etc...No idea about this.Is there any standard pattern  which i can just
 base and copy (example skin-yellow;breast-pink etc..)Im trying to surf in
 the net but cant find..Please someone help me???
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Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES

[Victoria Baker]

My Bad for not explaining fully - not all prostate biopsies do get PIN4,
additional sections are cut and saved for possible future requests on
prostate biopsies.  This cannot be done automatically for the very reason
you describe.  My intention was to point out that we separated these out so
that the cutters would know to cut the additional sections.

My apologies for not fully explaining this.

Vikki

On Fri, Mar 23, 2012 at 10:01 AM, Lester Raff MD lr...@uropartners.comwrote:

 Lavender - Prostate biopsies (short run also PIN4 built into protocol)

 No! No! NO!  This is why we are running into trouble on
 reimbursements!!!  PIN4 should NOT be done routinely. If you mean
 cutting a slide for potential PIN4, that's fine, but no pathologist
 should automatically be doing a PIN4 on every prostate biopsy

 Lester J. Raff, MD
 Medical Director
 UroPartners Laboratory
 2225 Enterprise Dr. Suite 2511
 Westchester, Il 60154
 Tel 708.486.0076
 Fax 708.492.0203


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria
 Baker
 Sent: Friday, March 23, 2012 8:53 AM
 To: Bernadette del Rosario
 Cc: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES

 Hi Bernadette,

 I know of several different color code set  ups.
 Pink - breast tissue (usually has separate processing cycle for
 ER/PR)
 Lt. Green - biopsy tissue (has separate processing cycle - short run)
 Tan -  bone marrow (has special stains and IHC built into the
 protocol)
 Lavender - Prostate biopsies (short run also PIN4 built into protocol)
 Aqua - skin/derm specimens
 Red - stat or rush specimens
 Grey - urate crystal (special processing)
 White - all other surgical material including breast tissue that does
 not
 require special protocols
 Blue - autopsy material

 In some labs they correspond the cassettes and the slides (green
 cassette =
 green slide).  In this context you will look at having both regular
 (superfrost) and plus slides available as well.

 There really isn't a set protocol it is more a way of easy
 identification
 for processing and also which case/specimen take priority.  Faster
 identification for loading of processors (breast tissue, biopsy,
 priority
 cases) ,embedding (priority and embedding orientation), cutting (special
 stains, add'l sections etc).

 I'm not fond of the red cassettes because they are difficult to read if
 not
 imprinted properly, but that color stood out best and most techs
 associated
 it with 'move it out fast'.  I did get a chuckle when a tech once asked
 me
 why we had lavender for prostate biopsies and I had to tell him that the
 blue had already been taken ;-).  One other thing that I had
 consternation
 about was putting fatty breast tissue from a reduction in white because
 of
 grossing/processing issues.  I could not get assistance through the
 Doc's
 for this so there were re-pro's until I was able to get ALL breast
 tissue
 put on the longer processing cycle.  It didn't make me a lot of friends
 in
 the grossing area though.

 For the biopsies you may want to be sure you have matching mesh
 cassettes.
 The lavender ones I usually always used mesh.

 Look at what your specimen types will be and associate them with
 something
 that is easily recognized by staff.  There isn't a set protocol for it
 though.  Sorry and I hope this helps.

 Vikki








 On Fri, Mar 23, 2012 at 8:42 AM, Bernadette del Rosario 
 badzros...@yahoo.com wrote:

  Good day histonetters.We are a  new university hospital and setting up
  histopatholology lab.I used to work with white biopsy cassettes only
 but
  not technicolors.Got this boss who ask me protocols on colored
 cassettes
  etc...No idea about this.Is there any standard pattern  which i can
 just
  base and copy (example skin-yellow;breast-pink etc..)Im trying to surf
 in
  the net but cant find..Please someone help me???
  ___
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Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES

[Victoria Baker]

No I understand fully what you are saying.  Have a great weekend.



On Fri, Mar 23, 2012 at 10:10 AM, Lester Raff MD lr...@uropartners.comwrote:

 Thanks for the explanation! I didn't mean to jump on anyone, after all,
 it's Friday!

 Lester J. Raff, MD
 Medical Director
 UroPartners Laboratory
 2225 Enterprise Dr. Suite 2511
 Westchester, Il 60154
 Tel 708.486.0076
 Fax 708.492.0203

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria
 Baker
  Sent: Friday, March 23, 2012 9:07 AM
 To: Lester Raff MD
 Cc: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES

 My Bad for not explaining fully - not all prostate biopsies do get PIN4,
 additional sections are cut and saved for possible future requests on
 prostate biopsies.  This cannot be done automatically for the very
 reason
 you describe.  My intention was to point out that we separated these out
 so
 that the cutters would know to cut the additional sections.

 My apologies for not fully explaining this.

 Vikki

 On Fri, Mar 23, 2012 at 10:01 AM, Lester Raff MD
 lr...@uropartners.comwrote:

  Lavender - Prostate biopsies (short run also PIN4 built into
 protocol)
 
  No! No! NO!  This is why we are running into trouble on
  reimbursements!!!  PIN4 should NOT be done routinely. If you mean
  cutting a slide for potential PIN4, that's fine, but no pathologist
  should automatically be doing a PIN4 on every prostate biopsy
 
  Lester J. Raff, MD
  Medical Director
  UroPartners Laboratory
  2225 Enterprise Dr. Suite 2511
  Westchester, Il 60154
  Tel 708.486.0076
  Fax 708.492.0203
 
 
  -Original Message-
  From: histonet-boun...@lists.utsouthwestern.edu
  [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
 Victoria
  Baker
  Sent: Friday, March 23, 2012 8:53 AM
  To: Bernadette del Rosario
  Cc: histonet@lists.utsouthwestern.edu
  Subject: Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES
 
  Hi Bernadette,
 
  I know of several different color code set  ups.
  Pink - breast tissue (usually has separate processing cycle for
  ER/PR)
  Lt. Green - biopsy tissue (has separate processing cycle - short run)
  Tan -  bone marrow (has special stains and IHC built into the
  protocol)
  Lavender - Prostate biopsies (short run also PIN4 built into protocol)
  Aqua - skin/derm specimens
  Red - stat or rush specimens
  Grey - urate crystal (special processing)
  White - all other surgical material including breast tissue that does
  not
  require special protocols
  Blue - autopsy material
 
  In some labs they correspond the cassettes and the slides (green
  cassette =
  green slide).  In this context you will look at having both regular
  (superfrost) and plus slides available as well.
 
  There really isn't a set protocol it is more a way of easy
  identification
  for processing and also which case/specimen take priority.  Faster
  identification for loading of processors (breast tissue, biopsy,
  priority
  cases) ,embedding (priority and embedding orientation), cutting
 (special
  stains, add'l sections etc).
 
  I'm not fond of the red cassettes because they are difficult to read
 if
  not
  imprinted properly, but that color stood out best and most techs
  associated
  it with 'move it out fast'.  I did get a chuckle when a tech once
 asked
  me
  why we had lavender for prostate biopsies and I had to tell him that
 the
  blue had already been taken ;-).  One other thing that I had
  consternation
  about was putting fatty breast tissue from a reduction in white
 because
  of
  grossing/processing issues.  I could not get assistance through the
  Doc's
  for this so there were re-pro's until I was able to get ALL breast
  tissue
  put on the longer processing cycle.  It didn't make me a lot of
 friends
  in
  the grossing area though.
 
  For the biopsies you may want to be sure you have matching mesh
  cassettes.
  The lavender ones I usually always used mesh.
 
  Look at what your specimen types will be and associate them with
  something
  that is easily recognized by staff.  There isn't a set protocol for it
  though.  Sorry and I hope this helps.
 
  Vikki
 
 
 
 
 
 
 
 
  On Fri, Mar 23, 2012 at 8:42 AM, Bernadette del Rosario 
  badzros...@yahoo.com wrote:
 
   Good day histonetters.We are a  new university hospital and setting
 up
   histopatholology lab.I used to work with white biopsy cassettes only
  but
   not technicolors.Got this boss who ask me protocols on colored
  cassettes
   etc...No idea about this.Is there any standard pattern  which i can
  just
   base and copy (example skin-yellow;breast-pink etc..)Im trying to
 surf
  in
   the net but cant find..Please someone help me???
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Re: [Histonet] April fool's prank

[Victoria Baker]

There are a few I remember from the past:

Telling the pathologists that the processor has been delayed or stopped
running in the alcohols.  If you ever want to see them go bonkers - that
one will do it.

Loosening or taking out of bulbs from microscopes - not a very nice thing
to do but I've heard of it.  Also make sure that the back up bulbs have
been safely secured elsewhere.  No I've not done this..but I'm told a
pathologist or tech without their microscope can be pretty intense.

There is always the sick call thing - but it has grown sort of old.

Having a pathologist read a fake slide.  We used an oak leaf - made out all
the paperwork and all of it tied into 'woody' things.  Had an unsuspecting
green tech gross it in and so on.  It's amazing what you can pull off on
newbies.

Put Vaseline on the microscope stage - slides will go 'slip sliddn' away as
they try to adjust x/y positions.

Tape or block (pennies sometimes work)  their top center desk drawer shut -
they can't open other drawers if that one won't open. (or am I dating
myself).

Replace sharp pencils and pens with dull pencils(stubs if possible) and
pens that don't work or are difficult.  There is always replacing their red
pen ink with blue or black ink.

A favorite is replacing a keyboard or loosening a connection on their
computer or dictation machine.  If it's a 'dead' keyboard they don't know
what to do initially - if it's a wrong keyboard it's even more fun.  PC vs.
MAC in the beginning they don't even look they just don't know why it won't
work.  It's before they've had the chance for their 5th cup of coffee.

For the techs -

Setting the microtome to 0 on older ones or changing the auto to not
advance.  I had trouble with the automated ones but found I could rig it
just right.  If ever practice made perfect - this was one, because I
learned alot on my own about automation there.


Have fun and be creative.  Lab work can be pretty intense - some good clean
laughs break the intensity.

























On Wed, Mar 21, 2012 at 11:27 AM, Breeden, Sara sbree...@nmda.nmsu.eduwrote:

 I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's
 eyepieces.  Vaseline on the coffee cup handles (don't all pathologists
 consume Mass Quantities of the stuff???).  A tray of completely blank
 slides, all properly numbered for the day's work?

 Feed me some good ones, too.  I'm down to my last week and I need to be
 remembered when I'm gone so they won't call me to do p.r.n.!

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Re: [Histonet] Undecalcified bone IHC

[Victoria Baker]

Hi Jeff,

If is it possible a few more specifics of how the tissue has been received,
processed and evaluated would help.  Undecalcified bone sectioning
procedures vary and also what specific markers are you looking to do is
important.

Vikki
On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa rjbu...@yahoo.com wrote:

 Undecalcified? How are you going to section it?
 If you can section it, just use any IHC protocol for regular sections.
 Good luck!
 René J.

 --- On Mon, 3/12/12, Jeffery Howery jeffery.how...@jcl.com wrote:


 From: Jeffery Howery jeffery.how...@jcl.com
 Subject: [Histonet] Undecalcified bone IHC
 To: histonet@lists.utsouthwestern.edu
 Date: Monday, March 12, 2012, 10:59 AM


 Does anyone have a protocol for Undecalcified bone for IHC?

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Re: [Histonet] Re: undecalcified bone IHC

[Victoria Baker]

Thank you Gayle.   Vikki
 On Mar 12, 2012 1:04 PM, gayle callis gayle.cal...@bresnan.net wrote:

 Jeff,



 It is most certainly possible to do IHC on undecalcifed bone sections
 embedded in PMMA although not the easiest task.   Sectioning is done on a
 microtome that is powerful enough to cut the plastic and using tungsten
 carbide knives.   The key is total removal of the plastic from MMA embedded
 bone sections to allow antibody/ immunoglobulins to access antigenic sites.
 Neil Hand has done IHC successfully on PMMA embedded tissues including
 undecalcified bone on 2 to 3 µm thick sections.  I think one could cut
 thicker sections at 4 to 5 µm and still be successful.  I do not recall
 what
 Troiano et al used.



 The following publications will help you and should include protocols,
 although conventional protocols will work according to Hand.



 Blythe D. Hand N et al 1997 J Clin Path 50:45-49.The use of methyl
 methacrylate resin for embedding bone marrow trephine biopsies.

 Hand NM et al 1996 Antigen unmasking using microwave heating on formalin
 fixed tissue embedded in methyl methacrylate J Cellular Path 1:31-37

 Jackson P et al.   1996  Amplification of immunocytochemical reactions by
 the catalytic deposition of biotin on tissue sections.   J Path
 170(suppl):23A.  This was about tyramide amplification when one gets a weak
 signal from conventional methods.

 Hand NM, Church RJ 1998 Superheating using pressure cooking: its use and
 application in unmasking antigens embedded in methyl methacrylate.  J
 Histotechnology 2`:231-236

 Hand NM et al 1989 Immunohistochemistry on resin embedded tissue for light
 microscopy: a novel post embedding procedure.  Proceeding Royal
 Microscopical Society 24(1):A54-55.

 Hand NM Plastic Embedding media and techniques, Ch.30, p 663-677.   Theory
 and Practice of Histological Technique,  5th edition by Gamble and
 Bancroft.
 The 6th edition is updated under same title.



 Use Google Scholar to find Troiano N et al from Yale on doing IHC on PMMA
 embedded bone sections with publications in J Histotechnology.





 Hand mentioned several HIER methods, using citrate buffer.   Optimizing
 retrieval will depend on the antigen and you may end up doing this with
 some
 form of HIER, including microwave or other heat producing methods and with
 different buffers. Enzyme digestion is also a possibility.



 Hand removed MMA with xylene, warm my speed up the removal, also more than
 one change for 10 - 20 minutes or longer.   When I talked to him
 personally,
 he said he had used warm xylene although temperature was not mentioned in
 his chapter.   After MMA removal, rehydrate section through alcohol
 gradient
 as one does paraffin sections.He was emphatic about never allowing the
 sections dry out.



 Hopefully Jack Ratliff and Damien Laudier will provide more insight on this
 topic.



 Good luck



 Gayle M. Callis

 HTL/HT/MT(ASCP)













 **



 Hi Jeff,



 If is it possible a few more specifics of how the tissue has been received,

 processed and evaluated would help.  Undecalcified bone sectioning

 procedures vary and also what specific markers are you looking to do is

 important.



 Vikki

 On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa rjbuesa @t yahoo.com
 wrote:



  Undecalcified? How are you going to section it?

  If you can section it, just use any IHC protocol for regular sections.

  Good luck!

  René J.

 

  --- On Mon, 3/12/12, Jeffery Howery Jeffery.Howery @t jcl.com wrote:

 

 

  From: Jeffery Howery Jeffery.Howery @t jcl.com

  Subject: [Histonet] Undecalcified bone IHC

  To: histonet @t lists.utsouthwestern.edu

  Date: Monday, March 12, 2012, 10:59 AM

 

 

  Does anyone have a protocol for Undecalcified bone for IHC?

 

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Re: [Histonet] Automatic HE stainer with coverslipper (glass)

[Victoria Baker]

Ventana Symphony - it has a lot of issues which Ventana has not completely
addressed.
On Aug 31, 2011 9:24 PM, Ernestine Middleton ernestinemiddle...@yahoo.ca
wrote:
 Hi;
 Looking and need comments on those of you that are using combination HE
stainer with glass coverslipper.
 Thank you.

 Ernestine Middleton, Manager,  HT, HTL ,BS ,MPA
 Montefiore Medical Center
 Bronx, New York
 718-920-4157
 emidd...@montefiore.org
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Re: RE: [Histonet] prostate bx containers

[Victoria Baker]

The sites are individual according to the specimen requisition form and on
the container.  It is in how the labeling is done.  Also when the
pathologist reads them out they will be reading by site/position.   I've
never used these containers but have received specimens in a single
container individually wrapped and identified where the pathologist read
them as they were.
Vikki
On Jul 11, 2011 1:17 PM, Jones, Laura lpjo...@srhs-pa.org wrote:
 We looked at these, but our Pathologist's first question was can we bill
for 6 or 8 bottles if they are technically all in one? How is that handled?

 
 From: histonet-boun...@lists.utsouthwestern.edu [
histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [
mjdess...@wvhcs.org]
 Sent: Monday, July 11, 2011 11:44 AM
 To: Sheila Adey; histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] prostate bx containers

 We don't currently use them but I've used them in the past. As long as you
don't 'bump' them on the grossing station while they're open, they're fine.
I have yet to see one leak.

 Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health
Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org |
 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax:
570-552-1526

 

 From: Sheila Adey [mailto:sa...@hotmail.ca]
 Sent: Sat 7/9/2011 9:36 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] prostate bx containers




 Hello:

 Has anyone used these prostate biopsy collection containers from Simport?
It looks like a petri dish and has 12 individual wells in it, each one
labelled with the location from the prostate.
 We are going to trial them but I would like some opinions on them.
 We currently get 10 to 12 bottles per case. So, all the bx's could go in
this one container.
 Thanks
 Sheila


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Re: [Histonet] RE: PAS oops...

[Victoria Baker]

I'm hoping that the attachment will attach.  It's an old procedure from the
1950's


Vikki

If this does not attach, please let me know.

http://jcs.biologists.org/content/s3-97/37/11.full.pdf

On Tue, Jun 28, 2011 at 10:47 AM, Elizabeth Chlipala l...@premierlab.comwrote:

 Chromic acid will work but I think that's for PAS for fungus.

 Liz

 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
 Manager
 Premier Laboratory, LLC
 PO Box 18592
 Boulder, CO 80308-1592
 (303) 682-3949 office
 (303) 682-9060 fax
 (303) 881-0763 cell
 www.premierlab.com

 Ship to address:

 1567 Skyway Drive, Unit E
 Longmont, CO 80504

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
 sgoe...@mirnarx.com
 Sent: Tuesday, June 28, 2011 8:36 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] PAS oops...

 So I dumped my periodic acid like a moron...does anyone know of another
 reagent that will oxidize for the PAS reaction?  Looks like I have
 acetic and hydrochloric acids...and that is all...hmm...



 Sarah Goebel-Dysart, BA, HT(ASCP)

 Histotechnologist

 Mirna Therapeutics

 2150 Woodward Street

 Suite 100

 Austin, Texas  78744

 (512)901-0900 ext. 6912



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Re: [Histonet] blades

[Victoria Baker]

I use the cardboards that come in a box of slides.  A small piece of tape on
the open side and mark it used.  I just always make sure I have the blade
edge facing the folded part.   I know some who will tape this folded
board it to the side of their microtome and use it as a trimming blade
holder.

Vikki



On Fri, Jun 24, 2011 at 5:10 PM, Esther C Peters epete...@gmu.edu wrote:

 We put ours in a small slide box (plastic or styrofoam, 5-25 slides) that
 is clearly marked Microtome Blades for Facing Blocks to be used another
 day.

 Esther C. Peters, Ph.D.
 Assistant Professor
 Department of Environmental Science  Policy
 Biology Program/Medical Technology Coordinator
 George Mason University
 4400 University Drive, MSN 5F2
 Fairfax, VA 22030-
 Office: David King Hall 3057
 Phone: 703-993-3462
 Fax: 703-993-1066
 epete...@gmu.edu

 - Original Message -
 From: Webb, Dorothy L dorothy.l.w...@healthpartners.com
 Date: Friday, June 24, 2011 4:53 pm
 Subject: [Histonet] blades

  Trying to clean up some things hanging out there in our lab and
  wondering what everyone does with a blade that has been used
  minimally and tech done for the day with the microtome.  Where do
  you store that blade for use tomorrow or do you toss and not worry
  about the cost involved?  I do not like them sitting on top of the
  microtome.  Any good ideas??  Thanks, as always!
 
 
 
   
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Re: [Histonet] Reticulin Stain(Gomori)

[Victoria Baker]

Polyscientific of Bayshore NY has a very simple kit.  You can find them on
line.
Vikki
On Jun 13, 2011 4:37 PM, Igor Deyneko igor.deyn...@gmail.com wrote:
 Dear Histonetters!
 I was wondering if anyone has a good working Gomori stain protocol for
 Reticulin fibers. i understand that it's a pretty routine procedure;
however
 no one in my lab has ever done it, so I'm wondering if there is a
 ready-to-use kit already or is it all from scratch. I'm trying to stain
 liver, spleens and bone marrows.Thank you very much in advance for any
 advise/help/protocols.
 Igor Deyneko
 Infinity Pharmaceuticals
 Cambridge, MA 02139
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Re: [Histonet] Re: Anatomicalalal

[Victoria Baker]

I've been reading all of this and just lovin' it!  Anatomic Pathology or
Clinical  Anatomic Pathology.  It was also once told to me that Anatomic
Pathology was the ugly step sister which is why getting $$'s was
difficult.
But I have another question is it Tol-U-i-dine blue or Tol-id-ei-on blue?
Tom-A-toe ,Tom-a-tow, Pot-A-toe, Pot-a-tow let's call the whole thing
off.  Isn't that how the song goes?  Sorry I couldn't resist.  Have a happy
night.  Sara my $5 is on it's way... now you can get an extra paddle for
your row boat!
Vikki
 On Jun 13, 2011 4:56 PM, Emily Sours talulahg...@gmail.com wrote:
 I'm going to start using this pronunciation. It's so hardcore!

 A great book should leave you with many experiences, and slightly
exhausted.
 You should live several lives while reading it.
 -William Styron



 On Mon, Jun 13, 2011 at 4:54 PM, Robert Richmond rsrichm...@gmail.com
wrote:



 Periodic acid is pronounced purr-eye-OH-dik. The word is derived
 from iodic (eye-OH-dik, derived from iodine), with the per-
 referring to a higher oxidation state, as in peroxide.

 Period.

 Bob Richmond
 Samurai Pathologist
 Knoxville TN

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Re: [Histonet] Re: Anatomicalalal

[Victoria Baker]

Thanks Bob.  English isn't my first language...so I sound things out a
little differently.
I've heard several different pronunciations in several different parts of
the east coast and mid- west.   I just had to have some fun.  Vikki
On Jun 13, 2011 6:02 PM, Robert Richmond rsrichm...@gmail.com wrote:
 Vikki Baker notes:

I've been reading all of this and just lovin' it! Anatomic Pathology or
Clinical  Anatomic Pathology. It was also once told to me that Anatomic
Pathology was the ugly step sister which is why getting $$'s was
difficult.
 But I have another question is it Tol-U-i-dine blue or Tol-id-ei-on
 blue? Tom-A-toe ,Tom-a-tow, Pot-A-toe, Pot-a-tow let's call the
 whole thing off. Isn't that how the song goes? Sorry I couldn't
 resist. Have a happy night. Sara my $5 is on it's way... now you can
 get an extra paddle for your row boat!
 Vikki

 Toluidine blue is tuh-LOO-uh-din or tuh-LYOO-uh-din depending on
 where you come from in the English language. I've also heard
 tuh-LOO-uh-deen.

 Anatomic pathology is indeed often referred to as the ugly
 stepsister or red-haired stepchild because of its isolated lowly
 position in the clinical laboratory, a situation that's gotten
 somewhat worse in my near half a century in pathology.

 Bob Richmond
 Samurai Pathologist
 Knoxville TN
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[Histonet] Special stains poster

[Victoria Baker]

 Hi

I think it was about six or seven years ago I had come across a company that
made a 'promotional' laminated poster of widely used special stains that
they had given to customers to show new techs and students how stains should
look.  I've had no luck in searching the internet so I'm hoping someone o
the histonet can help me.  I've got a group of tech's I'm working with that
have varying levels of experience and a poster like this would be a big help
to them.

If anyone has a source, please let me know.

Thank you in advance.

Vikki
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Re: [Histonet] back up power for VIPs?

[Victoria Baker]

Shelia-

I'm hoping that I'm reading this correctly.  You're currently on emergency
or designated line power, but you're experiencing power fluctuations which
trip up the processors.  If this is the case, I have encountered this and it
turned out that the engineering department was working on changing out
generators - but didn't inform us.  They were doing it on what they
considered off hours on the weekends right around the time our processors
would be just starting to process.  If you haven't check with them yet I
would and let them know what is happening.  When it first started to happen
the electrical people came and checked the outlets - which of course, showed
no problems.  They never told us about the generator changes, I had to get a
little ugly to find that out.

Not knowing what processors you have I can't answer about the back up
power.  I know that it is possible to purchase stand alone generators that
can be installed and will provide power in emergency situations but they are
expensive and have limitations as to how long they can sustain power.  It's
been about two years since I looked into them so things may have changed.

Hope this of some help.

Vikki

On Fri, May 27, 2011 at 9:44 PM, Sheila Adey sa...@hotmail.ca wrote:


 Hello everyone:

 We are experiencing lots of quick power outages at work that keeps setting
 the processors into power outage alarms.
 Can anyone recommend a back up power product. I was told we can't put them
 on battery back up b/c of their power requirements???
 They are on emergency power.

 Thanks for your help in advance. :)

 Sheila
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[Histonet] Use of EDTA to soften tissue (non - calcified)

[Victoria Baker]

Good morning,

I'm a little stumped here - no it's not a first I know.

I've come across a lab that uses EDTA powder in their ice baths to soften GI
biopsies - I've never heard of this before. I've used it for bone or
calcifications when nothing else was available for a surface decal and
didn't like how it would sometimes affect staining.

Thanks in advance for any information.

Vikki
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Re: [Histonet] p16ink4a

[Victoria Baker]

Go to www.mtmlabs.com.  They have the patent.
Vikki
On Mar 11, 2011 10:55 AM, Phyllis Thaxton dch...@yahoo.com wrote:
 Hi All,

  Is anyone using this P16INK4A  for IHC?  I searched the archives and
found some
 threads from 2009 with links  that do not work for possible suppliers. I
also
 found some for ELISA and Western Blot, and Flow Cytometry. Is this
antibody used
 at all in FFPE tissue?
  Phyllis Thaxton HT(ASCP)QIHC
 DCH Regional Medical Center
 Tuscaloosa, AL



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Re: [Histonet] Traveling Histotechs

[Victoria Baker]

Aureus Medical Staffing - Omaha NE
Club Staffing - FL
TravelMax - not sure where they are located
Ampian - Nevada
Full Staff - TX
CompHealth - not sure which site does travel work at this point

These are just a few of the many I'm sure.

Vikki


On Wed, Mar 2, 2011 at 12:37 AM, Jennifer MacDonald jmacdon...@mtsac.eduwrote:

 Does anyone have information on any agencies that specialize in positions
 for traveling histotechs?
 Thank you,
 Jennifer MacDonald
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Re: RE: [Histonet] parts for CV5030 glass coverslipper

[Victoria Baker]

Have you tried contacting Belair Instruments?  I believe they are located in
Fanwood NJ.
Angie if you get a chance contact me off server :-).
On Feb 28, 2011 11:04 AM, Sherwood, Margaret msherw...@partners.org
wrote:
 We have a refurbished CV5030 coverslipper and we ordered directly from
Leica.
 If someone knows of anyone else who sells the parts/supplies, I would also
be
 interested.

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
 Sent: Monday, February 28, 2011 10:55 AM
 To: histonet
 Subject: [Histonet] parts for CV5030 glass coverslipper

 Does anyone know if you can buy these from someone else besides Leica? My
Fisher
 rep never got back to me.


 Angela Bitting, HT(ASCP), QIHC
 Technical Specialist, Histology
 Geisinger Medical Center
 100 N Academy Ave. MC 23-00
 Danville, PA 17822
 phone 570-214-9634
 fax 570-271-5916

 No trees were hurt in the sending of this email
 However many electrons were severly inconvienienced!


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Re: [Histonet] IHC Equipment

[Victoria Baker]

In regards to the Ventana Ultra - if you are running PIN-4 or ISH
simultaneously on this instrument, the kits/ab's/probes  they require take
up a lot of space on the reagent carousel which limits the number of
antibodies you can put on and as runs can go some where around 6-hours you
could find yourself limited in what you can run without careful planning.

I'm a long time user and believer in Ventana, but BondMax and Intellipath do
have my loyalties as well.  It depends on the needs of your lab (volume/work
flow, available lab space and antibody library size), the staffing you have
available and their abilities with IHC/ISH.  I've done this before and the
only way to really know if an instrument will work in your lab is to
demo them and connect with other users of the instrument.

Vikki






On Fri, Feb 25, 2011 at 9:30 AM, Greg Dobbin gvdob...@ihis.org wrote:

 The Bond does do ISH. In fact it utilizes the same detection kit so you
 have only to buy the probes, not the additional detection kit that could
 (depending on your volume of ISH requests) expire.
 Greg

 Greg Dobbin, R.T.
 Chief Technologist, Anatomic Pathology
 Dept. of Laboratory Medicine,
 Queen Elizabeth Hospital,
 P.O. Box 6600
 Charlottetown, PEC1A 8T5
 Phone: (902) 894-2337
 Fax: (902) 894-2385

 I find that the harder I work, the
 more luck I seem to have.
 - Thomas Jefferson


  Sheila Fonner sfon...@labpath.com 2/25/2011 10:20 AM 
  Cindy,

 We used to use the Dako stainer, and we still have it as a back-up if
 necessary, but we have recently (in the last year) bought a Ventana Ultra.
 You can put 30 slides at a time on it, but you do not have to batch the
 slides.  It is a continuous feed machine, which means that as soon as a
 slide is done, you can take it off and run something else.  You do not have
 to have all of the slides from a case together side by side.  It is
 bar-code
 driven and will find the slides no matter where you put them.  Each slide
 drawer runs independently of the others.  Ours has been wonderful.  The
 technical assistance is fantastic also.  They will help you to initially
 work up all of your antibodies.  You can use theirs, or you can use third
 party antibodies and place them in a prep-kit.  You can also use RTU or
 concentrates.  It's really up to you.  I would highly recommend it if you
 have a large volume.  We demo'd the Biocare Intellipath also.  I liked the
 machine, but it was really just a step up from the Dako.  Leica has the
 Bond
 instruments, which a lot of people like, but for us, it didn't work because
 we wanted to be able to do ISH.  Also, the Leica limits you to drawers of
 ten slides.  So when you load a drawer, with 1 slide or 10, you can't use
 it
 again until that run is finished.  Hope this helps.  If you have any
 questions, you can contact me.
 Have a great day!

 Sheila


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia
 Pyse
 Sent: Wednesday, February 23, 2011 2:37 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] IHC Equipment

 Hi Histonetters

 I currently use a Dako stainer for my IHC staining. It is a work horse with
 very little problems. It is a older model that we may need to replace in
 the
 near future. What is everyone using out in histoland. I would be perfectly
 willing to purchase another Dako but I want to explore all avenues before
 making a decision. What are the pros and cons of the instruments any of you
 are using. How often is the machine down? What is the capacity? We run the
 Dako twice daily usually to the capacity of 48 slides. I would like to hear
 only from actual user of the instrumentation, no vendors please. This is
 only a fact finding e-mail. Thanks in advance for all your input.

 Cindy



 Cindy Pyse, CLT, HT (ASCP)

 Laboratory/Histology Supervisor

 X-Cell Laboratories

 716-250-9235

 e-mail cp...@x-celllab.com





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 If you are not the intended recipient, you are not authorized to use,
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Re: [Histonet] IHC Equipment

[Victoria Baker]

Sheila

It is impossible to always get everything into an e-mail when you recieve a
general info request.  I thought what you said was great.

Have a wonderful weekend.

Vikki

On Fri, Feb 25, 2011 at 10:25 AM, Sheila Fonner sfon...@labpath.com wrote:

  Vikki,



 You are absolutely right!  We do all of our ISH as overnight runs to avoid
 the problem of space constraint with the detection kits, etc.  I agree that
 all of the instruments have their good and bad points, and it really depends
 on the lab and what kind of specimens/volume you have.



 Didn’t mean to offend anyone.  I REALLY was just trying to help and give my
 opinion based on my own personal use.



 Sheila





 *From:* Victoria Baker [mailto:bakevicto...@gmail.com]
 *Sent:* Friday, February 25, 2011 10:18 AM
 *To:* Greg Dobbin
 *Cc:* Sheila Fonner; histonet@lists.utsouthwestern.edu; Cynthia Pyse
 *Subject:* Re: [Histonet] IHC Equipment



 In regards to the Ventana Ultra - if you are running PIN-4 or ISH
 simultaneously on this instrument, the kits/ab's/probes  they require take
 up a lot of space on the reagent carousel which limits the number of
 antibodies you can put on and as runs can go some where around 6-hours you
 could find yourself limited in what you can run without careful planning.



 I'm a long time user and believer in Ventana, but BondMax and Intellipath
 do have my loyalties as well.  It depends on the needs of your lab
 (volume/work flow, available lab space and antibody library size), the
 staffing you have available and their abilities with IHC/ISH.  I've done
 this before and the only way to really know if an instrument will work in
 your lab is to demo them and connect with other users of the instrument.



 Vikki












 On Fri, Feb 25, 2011 at 9:30 AM, Greg Dobbin gvdob...@ihis.org wrote:

 The Bond does do ISH. In fact it utilizes the same detection kit so you
 have only to buy the probes, not the additional detection kit that could
 (depending on your volume of ISH requests) expire.
 Greg

 Greg Dobbin, R.T.
 Chief Technologist, Anatomic Pathology
 Dept. of Laboratory Medicine,
 Queen Elizabeth Hospital,
 P.O. Box 6600
 Charlottetown, PEC1A 8T5
 Phone: (902) 894-2337
 Fax: (902) 894-2385

 I find that the harder I work, the
 more luck I seem to have.
 - Thomas Jefferson


  Sheila Fonner sfon...@labpath.com 2/25/2011 10:20 AM 

 Cindy,

 We used to use the Dako stainer, and we still have it as a back-up if
 necessary, but we have recently (in the last year) bought a Ventana Ultra.
 You can put 30 slides at a time on it, but you do not have to batch the
 slides.  It is a continuous feed machine, which means that as soon as a
 slide is done, you can take it off and run something else.  You do not have
 to have all of the slides from a case together side by side.  It is
 bar-code
 driven and will find the slides no matter where you put them.  Each slide
 drawer runs independently of the others.  Ours has been wonderful.  The
 technical assistance is fantastic also.  They will help you to initially
 work up all of your antibodies.  You can use theirs, or you can use third
 party antibodies and place them in a prep-kit.  You can also use RTU or
 concentrates.  It's really up to you.  I would highly recommend it if you
 have a large volume.  We demo'd the Biocare Intellipath also.  I liked the
 machine, but it was really just a step up from the Dako.  Leica has the
 Bond
 instruments, which a lot of people like, but for us, it didn't work because
 we wanted to be able to do ISH.  Also, the Leica limits you to drawers of
 ten slides.  So when you load a drawer, with 1 slide or 10, you can't use
 it
 again until that run is finished.  Hope this helps.  If you have any
 questions, you can contact me.
 Have a great day!

 Sheila


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia
 Pyse
 Sent: Wednesday, February 23, 2011 2:37 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] IHC Equipment

 Hi Histonetters

 I currently use a Dako stainer for my IHC staining. It is a work horse with
 very little problems. It is a older model that we may need to replace in
 the
 near future. What is everyone using out in histoland. I would be perfectly
 willing to purchase another Dako but I want to explore all avenues before
 making a decision. What are the pros and cons of the instruments any of you
 are using. How often is the machine down? What is the capacity? We run the
 Dako twice daily usually to the capacity of 48 slides. I would like to hear
 only from actual user of the instrumentation, no vendors please. This is
 only a fact finding e-mail. Thanks in advance for all your input.

 Cindy



 Cindy Pyse, CLT, HT (ASCP)

 Laboratory/Histology Supervisor

 X-Cell Laboratories

 716-250-9235

 e-mail cp...@x-celllab.com





 ___
 Histonet mailing list
 Histonet

Re: [Histonet] Checks and balances for specimen accessioning

[Victoria Baker]

Do you recall the source of pick up for the specimen?  If it is picked up by
Histology either in the OR or a lab site the pick up person should be
checking the name on requisition, container and label in the book or ledger
prior to initialing and dating.  Also the person bringing the specimen to
these sites has to initial and date when they put the pt. label in the
ledger.  I would go back to the source - as a starting point.  Once the
specimen is received into the lab, the person accessioning is checking the
container against the requisition.  Once a patient is registered either in
the hospital or at the site they are assigned a medical record number or
another unique identifier.  When you accession this patient by one of these
identifiers the descrepancy should (I stress should) show up and be caught.
Another check is to see if the specimen on the container matches what is on
the requisition.  Something which should be done from pick up point.
It varies by site how the specimen is dictated in regards to specimen ID.
Some read from the bottle while looking at the requisition and dictate from
that, but I've found it isn't always the rule.

The original label (source label) has to be clearly visible and not covered
with another label at any time in the process.  If you are using a barcode
label in the Histology lab the barcode needs to be placed way from the
source label.

The LEAN process does indeed standardize a lot of the work process, but it
is also based on the lab volume, work flow which is individual to each lab.
Also the use of computer systems and how fully they are utilized within each
lab.  I've worked in several labs that are LEAN and they still had issues
with specimen handling/verification  for different reasons.

In your situation you would have to follow the paper trail at this point,
but it would be a very strong reason to work towards getting a unified
system in place that tracks specimens from the source to final sign out.  I
know that there are companies out there that do this one is PathCentral they
provide software and support.

Have a great weekend.

Vikki











On Fri, Feb 25, 2011 at 11:00 AM, Scott, Allison D 
allison_sc...@hchd.tmc.edu wrote:

 Hello to all in histoland.  What types of checks and balances do you
 have in place for specimen accessioning.  We had a incidcent where I was
 accessioning a case and I did not catch that the name on the container
 was different from the name on the requisition.  The resident grossing
 did not catch it either.  They usually peel back the copath label and
 look at the name on the label that came from the procedure area.  In my
 case the resident did not do this. It was not until the pathologist saw
 a discrepancy in the age on the requisition and what was written in the
 pertinent history, that it was determined that it had been mislabeled
 from the beginning.  I did a incident report and the area was cited.
 Besides making sure that who ever is accesioning cases checks that the
 names match, what else can be done?  Any help in this will be greatly
 appreciated.

 Allison Scott HT(ASCP)
 Histology Supervisor
 LBJ Hospital
 Houston, Texas
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Re: [Histonet] Wage question for Wisconsin

[Victoria Baker]

Michael,

ASCP has a wage/salary survey that they distribute based upon region and
certification.  Some have said that this is biased as not everyone does
participate.  I tend to agree with the survey, but also recognize that
dermatopathology is a 'specialty' type lab.  I worked in Dermpath at NYU
back in the 80's to early 90's and our salaries were not identical to the
clinical labs as we were a part of the medical school and not the hospital.
Yes the hospital was 1199 at that time, I cannot say if they are still.

My recommendation is to go with what you can find from the survey, but also
network with other dermpath or other specialty labs in your area that you
may know or who you can connect with through others - either on the
listserver or off - to possibly get a better sounding of salaries.  HR at
your hospital might be also able to help if you have someone there that you
can work with and trust to obtain some of the data you're looking for and
understands what your specific needs are within the lab.  I would include
your pathologists in this as much as possible as they will be needed to
support your request(s) as they are essentially your clients.

Vikki

On Tue, Feb 15, 2011 at 6:02 PM, Michael Hillmer mhill...@dermwisconsin.com
 wrote:

 We are dermatology clinic in Northeast Wisconsin and we are trying to do
 gather accurate wage data.  Can anybody offer wages for an HT, HtL and
 lab assistants?  Any help would be greatly appreciated.









 Thank you-



 Michael Hillmer PHR

 HR Coordinator

 Dermatology Associates of Wisconsin

 Phone: (920)683-5278

 Fax: (920)686-9674

 Cell: (920)860-6360



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Re: [Histonet] Section position on slides

[Victoria Baker]

Automated coverslipping instruments are defined by the manufacturer, but
modifications on some systems can be done.  Most clinical labs who utilize
automation use 24X50mm - as this coverslip will cover most of the slide.

Tissue placement is pretty much determined by laboratory needs.  Sheehan 
Hrapchak had a short segment about tissue placement, but it was in regards
to the tissue orientation for microscopic assessment requirements.  There is
nothing in granite about tissue placement that I know of.

Some tools that I worked with were using the Cytospin large rectangular
slides to show residents/students/collegues where the tissue placement
needed to be.  Once they were able to get the tissue section in there we
moved on.  While to most of us who work in Histo sort of take putting
sections on a slide for granted (yes we did work hard to get there)  getting
the mechanics of it takes patience, skill and practice.  Keep it simple and
build up from there.

Vikki


On Wed, Feb 16, 2011 at 12:27 PM, Helen Fedor hfe...@jhmi.edu wrote:

 Since automation is becoming more and more a part of all Histology labs the
 demands of placement of the tissue on the slides varies for different
 instruments. Stainers, coverslippers and now with slide scanning as well. So
 I do not believe that there is a silver bullet answer.

 Helen L. Fedor

 Tissue Microarray Lab, Manager
 Prostate Spore Lab, Manager
 Johns Hopkins University
 600 N. Wolfe St, | Marburg Room 406
 Baltimore, MD | 21287-7065

 410.614.1660


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine
 (CDC/OID/NCEZID)
 Sent: Wednesday, February 16, 2011 12:21 PM
 To: Tanya Ewing-Finchem; histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Section position on slides

 I always try to center the section equally from sides and top-bottom as
 possible. This means measure from the bottom of the frosted edge as the
 top.  The Artisan special stains system has a clip that attaches around
 the slide to allow reagents to pool onto the sections and incubate.  If the
 section is too close to the sides then these areas do not stain adequately.
  Sometimes if the tissue section itself is very large this is unavoidable.
  With automated coverslippers you must also consider placement of tissue to
 allow for proper coverage.

 If I am cutting multiple unstained slides for subsequent testing I try to
 orient the tissue the same on each slide to facilitate the reading of these
 slides by the pathologist's

 Jeanine Bartlett
 Infectious Diseases Pathology Branch
 (404) 639-3590
 jeanine.bartl...@cdc.hhs.gov

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tanya
 Ewing-Finchem
 Sent: Wednesday, February 16, 2011 12:09 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Section position on slides


 I am trying to put together a training document around microtomy and
 sectioning and am finding it hard to find information around the placement
 of the actual sections on the slides.  These are the objectives I am looking
 to answer.  Is this information found in any publications?

 1)  Tissue / Section Placement:  Are there published guidelines /
 documentation on precisely where you should place tissue sections on a 25mm
 x 75mm glass slide?   Perhaps more importantly, where you should NOT place
 tissue (ie. x mm from the edge of the glass slide)?

 2)  Diagnosable Slide Staining Area:  With automation becoming more widely
 used in IHC, are there published guidelines / documentation on the usable or
 diagnosable staining area on a 25mm x 75mm glass slide?  For instance, would
 you define that as the area under a traditional coverslip?  Would this be
 defined as the entire slide below the label?  Or is this some distance from
 all the edges of the slide?  With some automated systems, it is near
 impossible to get edge to edge staining.  Is this acceptable?



 Thanks for any ideas.
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Re: [Histonet] p16 antibody

[Victoria Baker]

Yes.  They hold the patent.
On Feb 10, 2011 1:30 PM, Michele Carr michelecar...@yahoo.com wrote:
 Hi everyone was wondering who carries the IVD p16 antibody? Is it only
MTM?
 Thanks in advance.
 Michele Carr HTL ASCP
 Medical Laboratory Services




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Re: [Histonet] core histolgy recharge rates

[Victoria Baker]

Hi-
A flat fee per slide was charged which included all *technical* preparation
for the final slide.  Also prior to the start of a project a cost estimate
was compiled and submitted for review/approval.  As portions of projects
were completed we submit via the grant or project number the work completed
and $$'s were deducted from that cost center.

We did do a number of different types of tests/assays and if we were
approached for a new type of assay then we would have to put together a cost
analysis based on the needs of the project.  Incorporated into that cost
would be the technical/labor component also including whatever overhead
percentage was required by the facility.

This worked out well as I had everything done 'up front' and knew what was
budgeted.  We did have to submit a quarterly report of all work/$$'s to the
government and by doing a flat fee it made life much easier.

Vikki

On Wed, Feb 9, 2011 at 1:59 PM, Michael Mashore mmash...@vapop.ucsd.eduwrote:

 Hello all,

 I am trying to get a feel for how other core histology facilities are
 charging. My main question is if or when technician's labor time is
 charged.
 Currently we charge a fee per sample for embedding, whether it is paraffin,
 plastic, or oct. Then we charge a fee per slide/grid for cutting, and fee
 per slide/grid for staining. Any time the technician is doing the work
 there
 is also a labor/tech time fee per hour.

 Any information you are willing to share is very much appreciated.

 Thanks,

 Michael




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Re: [Histonet] High Complexity Testing

[Victoria Baker]

The performance of ISH/IHC is in many laboratories considered high
complexity testing - however - as the technologist doing the work does not
make the diagnosis it is considered to be a part of specimen processing.
(How's that for political double talking!)   Use of automation in these
procedures provides standardization of the protocols, but it is still a
'machine' it would not know how to recognize a staining or processing issue
along with other possible faults.  The purpose of automation was to provide
standardization and consistent reproducibility of protocols, reduce
antibody/reagent amounts, free the technologist for other needs in the lab
and reduce turn around time required for this procedure.  In essence it is
hands off BUT not brains off.

Short history is that when CLIA and CAP first started doing these ratings of
low - high complexity testing Histology was a grey/special area in that the
staff performed the technical aspect of the work, but did not sign off or
make the diagnosis.  Since then we have had revisions in CLIA and even with
CAP guidelines there is still an aspect of subjectivity based upon the
inspector's interpretation of the guidelines.  It's frustrating, infuriating
at and at times totally exasperating as you are trying to comply with all
the requirements and regulations that are supposed to be clear.

If your pathologist is considering all Histology work as low complexity I
would wonder why they thought that?  Is the lab participating in any of the
CAP QA/QI programs?  Does he/she participate in doing any In services with
the staff?  Do they interact with the staff and share anything  with them in
regards to the work or any specific concerns they may have?  Does the
department have reference material available to the staff for further study
or understanding of the work they do and how important it is to patient
care?  Is the department willing to assist with $$'s for in-house CE courses
or local Histology societies to further the laboratory staff development?
One thing that I would wonder most is if they think that what you do is 'low
complexity' could they go into the lab and perform the work themselves?

Okay my morning rant is complete --- now I guess I should take my Estrogen
;-)

If I have offended anyone, I apologize in advance.  Hope everyone has a nice
day.

Vikki
On Tue, Feb 8, 2011 at 10:06 AM, O'Donnell, Bill 
billodonn...@catholichealth.net wrote:

 I don't actually have an answer, but rather an observation.

 How many med techs are still doing glusoses in test-tubes, or manual
 drug screenings or hcg's? It would seem, by deduction that an automated
 glucose, if only because who it performing it, is a complex test. If
 measuring a bowel biopsy is now complex, why should the critical
 judgements and skills needed to cut a section, place it correctly on a
 slide and load the machine be considered not complex.

 If it comes down to who is doing it, then all of our efforts to
 elevate the field, gain higher and more competative wages and education
 requirements have done little except in isolated regions or
 laboratories.

 Morning rant Need to start getting more sleep.

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila
 Fonner
 Sent: Tuesday, February 08, 2011 6:45 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] High Complexity Testing

  Hello All,



 I would really appreciate it if anyone has information on whether
 IHC/ISH are considered high complexity testing for histotechs.  Our
 pathologist believes that ALL histology low complexity testing since a
 machine is doing the work.  Can anyone help me out with some
 guidelines, literature, etc. that says otherwise?  I would really
 appreciate it.  We just want to know which one it is.



 Thanks so much Histoland!







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Re: [Histonet] High Complexity Testing

[Victoria Baker]

I should not have included CLIA in my e-mail as it would seem it has clouded
things a little.  I do apologize.  Initially when these issues and
guidelines came about CLIA and CAP dovetailed as far as Histology  was
concerned.

Shelia you were looking for contacts that would help you with getting a more
solid base to meet these regulations.  If you go to the CAP website and
click on the IHC link you will find links and publications to assist you.  I
would recommend that you contact the Applied Immunohistochemistry society as
well.  NSH or your state/regional society may also have additional
information.

Should I see something else in my searches I will most willingly forwarded
them to you.

Vikki

On Tue, Feb 8, 2011 at 12:43 PM, Whitaker, Bonnie bonnie.whita...@osumc.edu
 wrote:

 Hi All,

 There is a difference in performing a task (immunostaining) that is
 complex, and performing high complexity testing as the CLIA regulations
 govern.

 Yes, staining is a complex task, and it requires knowlegable techs to
 ensure that it is properly done, and to troubleshoot  difficulties when
 necessary.

 It is high complexity testing because testing personnel in anatomic
 pathology are pathologists (and the non-physician people performing gross
 examinations, who must meet high complexity testing personnel
 requirements.

 Testing personnel as defined by CLIA, are the people that report results
 of that test, not people who perform other related duties.

 That's my explanation of the whole mess.

 Bonnie Whitaker
 AP Operations Director
 Ohio State University Medical Center
 Department of Pathology
 614.293.5048


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
 Sent: Tuesday, February 08, 2011 12:22 PM
 To: Horn, Hazel V; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu;
 Sheila Fonner
 Subject: RE: [Histonet] High Complexity Testing

 I must disagree with this assessment of what makes a test complex.  If the
 test is done properly [the responsibility of the technologist] then the
 reading to the test is a visual determination that requires experience on
 the part of the pathologist, but if the test is not done properly, will the
 pathologist be able to tell the technologist what to do to fix the problem?

 Where's the Tylenol?


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
 Sent: Tuesday, February 08, 2011 9:58 AM
 To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner
 Subject: RE: [Histonet] High Complexity Testing

 While the test is high complexity it is the READING of the test by the
 pathologist that determines its complexity.  Because histotechs do not
 report the results our part of this test is not high complexity.

 Hazel Horn
 Hazel Horn, HT/HTL (ASCP)
 Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital
 1 Children's WaySlot 820
 Little Rock, AR   72202

 phone   501.364.4240
 fax501.364.3155

 visit us on the web at:www.archildrens.org

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
 Sent: Tuesday, February 08, 2011 10:32 AM
 To: histonet@lists.utsouthwestern.edu; Sheila Fonner
 Subject: Re: [Histonet] High Complexity Testing

 When a machine is doing the test, there are stringent provisions as to
 the preparation and validations of the test.
 Done manually, it requires a trained technologists and, yes, they are high
 complexity tests (both IHC and FISH, and their variations).
 René J.

 --- On Tue, 2/8/11, Sheila Fonner sfon...@labpath.com wrote:


 From: Sheila Fonner sfon...@labpath.com
 Subject: [Histonet] High Complexity Testing
 To: histonet@lists.utsouthwestern.edu
 Date: Tuesday, February 8, 2011, 7:45 AM


 Hello All,



 I would really appreciate it if anyone has information on whether IHC/ISH
 are considered high complexity testing for histotechs.  Our pathologist
 believes that ALL histology low complexity testing since a machine is
 doing the work.  Can anyone help me out with some guidelines, literature,
 etc. that says otherwise?  I would really appreciate it.  We just want to
 know which one it is.



 Thanks so much Histoland!







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Re: [Histonet] Infectious waste

[Victoria Baker]

Hi

You may receive many opinions on this --- just a heads up.  Many clinical
labs do not consider shavings or debris from cutting to be red bag
(hazardous) waste as they are no longer a 'biological hazard' due to the
processing process which incorporates heat, alcohol and a fixative that is
expected to effectively kill any infectious agents. There are some
exceptions, which you can find in the CDC or OSHA guidelines such as dealing
with tissue of brain (Jakob-creutzfeldt) lung (TB or other air-borne
pathogens).  CAP also has requirements/guidelines for handling
these cases which you will need to have documentation for the processing
procedure.  I would suggest that you refer to these guidelines and also to
your institutions regulations on what is regarded as hazardous waste.  Above
and beyond anything else make sure you document and site in your procedures
manual your sources.

Hope this will help you.

Vikki

PS - any spelling errors I apologize for in advance ;-)
On Tue, Feb 1, 2011 at 11:45 AM, Martin, Gary gmar...@marshallmedical.orgwrote

 We are wondering if paraffin block shavings are considered infectious
 waste.

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Re: [Histonet] Archives

[Victoria Baker]

Hi - I go by subject matter, but there was a time (a very long time ago)
where they had them by date as well.  When in doubt I just wing it and
somehow I find what I'm looking for.

Vikki

On Jan 24, 2011 7:14 PM, Joe Nocito jnoc...@satx.rr.com wrote:
 Greetings all,
 I'm going to show my ignorance (like that hasn't happened before). When
accessing the archives, how do I do it? Do I search by topic or date? All
the time I've been on here, I never had to use the archives. Thanks. Oh Oh,
I feel another tear coming

 Joe
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Re: [Histonet] History of Histology

[Victoria Baker]

Thophil Mitchell Prudden A Manuel of Normal Histology  published in 1881.

Vikki

On Tue, Jan 4, 2011 at 9:41 AM, Geoff McAuliffe mcaul...@umdnj.edu wrote:

 Brian Bracegirdle wrote a good book on the history of histotechnology.

 Geoff


 Barone, Carol wrote:

 HistonettersI am looking for the person who wrote History of
 Histology. I believe the author's name was either Mitchell or Michell?
 I found it on the web once, but have lost my copy? Anyone out there
 remember this article? CB
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 --
 --
 **
 Geoff McAuliffe, Ph.D.
 Neuroscience and Cell Biology
 Robert Wood Johnson Medical School
 675 Hoes Lane, Piscataway, NJ 08854
 voice: (732)-235-4583 mcaul...@umdnj.edu
 **




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Re: [Histonet] History of Histology

[Victoria Baker]

Sorry typo Theophil is the correct spellingneed more tea!

On Tue, Jan 4, 2011 at 10:34 AM, Victoria Baker bakevicto...@gmail.comwrote:

 Thophil Mitchell Prudden A Manuel of Normal Histology  published in 1881.

 Vikki

   On Tue, Jan 4, 2011 at 9:41 AM, Geoff McAuliffe mcaul...@umdnj.eduwrote:

 Brian Bracegirdle wrote a good book on the history of histotechnology.

 Geoff


 Barone, Carol wrote:

 HistonettersI am looking for the person who wrote History of
 Histology. I believe the author's name was either Mitchell or Michell?
 I found it on the web once, but have lost my copy? Anyone out there
 remember this article? CB
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 --
 --
 **
 Geoff McAuliffe, Ph.D.
 Neuroscience and Cell Biology
 Robert Wood Johnson Medical School
 675 Hoes Lane, Piscataway, NJ 08854
 voice: (732)-235-4583 mcaul...@umdnj.edu
 **




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Re: [Histonet] Use of ammonia water

[Victoria Baker]

Hi Sharon,

I use a 1.5% solution for bone marrow cases and bloody tissue.  It does also
work well for GI.  I've never had an issue with it affecting any of my IHC.
An alternative though (when I can't get my hands on ammonium hydroxide) is
to put these blocks in warm water (I'm careful about the waterbath because
of X-contamination issues) for about 30-45 seconds and this works just as
well.  I stopped using any surface decal solution or Nair as I did see some
differences with the expression of certain markers - I did not see any false
negs, but the staining itself was not as intense it looked like.

Vikki

On Mon, Dec 27, 2010 at 3:58 PM, Sharon.Davis-Devine 
sharon.davis-dev...@carle.com wrote:

 Happy Holidays Histonetters, I have a question for you. Do any of you use
 ammonia water to soften the blocks before cutting? If so, does this have any
 effect  on IHC staining? Any help will be greatly appreciated. Thanks.

 Sharon Davis-Devine, CT (ASCP)
 Cytology-Histology  Supervisor
 Carle Foundation Hospital
 Laboratory and Pathology Services
 611 West Park Street
 Urbana, Illinois 61801
 217-383-3572
 sharon.davis-dev...@carle.com

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Re: [Histonet] RAGE and AGE

[Victoria Baker]

Now they have that in a musical which is absolutely hysterical - Menopause -
The Musical.  When it comes to your area it is a must see for any going
through this wonderful stage in life.

Vikki

On Fri, Nov 12, 2010 at 1:24 PM, Heath, Nancy L. nhe...@lifespan.orgwrote:

 RAGE and AGE - yes rage gets worse with age...it's called menopausei
 just had too it's FRIDAY!!

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Annette
 Featherstone
 Sent: Friday, November 12, 2010 12:19 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] RAGE and AGE

 Is anyone using RAGE or AGE antibody successfully? If so would you be
 willing to share vendor and data?

 I am also looking for a Tunel Assay kit, what would any of you
 recommend?

 Thanks,

 Annette Featherstone

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[Histonet] negative controls

[Victoria Baker]

Hi
I have a hypothetical question to those who run IHC on Ventana instruments.
Are you running your negatives with your patient/test cases or on a separate
run?  Also, if you are doing this and have to use a different detection kit
how do you work the QA/QC portion of this for CAP requirements.

Thanks

Vikki
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Re: [Histonet] inking dyes

[Victoria Baker]

Try the EMS website I find them useful and their pricing is competative.
On Thu, Jul 15, 2010 at 10:52 AM, Diana McCaig dmcc...@ckha.on.ca wrote:

 Can someone provide me with a supplier of tissue marking dyes for use on
 the grossing bench.

 Diana McCaig
 Histology Lab
 Chatham Kent Health Alliance
 80 Grand Avenue West
 Chatham. Ontario
 N7L 1B7
 519-352-6401  (6604)

 This email communication and any files transmitted with it may contain
 confidential and or proprietary information and is provided for the use
 of the intended recipient only. Any review, retransmission or
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 recipient is prohibited. If you receive this email in error, please
 contact the sender and delete this communication and any copies
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Re: [Histonet] Moving across the country...

[Victoria Baker]

Jennifer,

If you are looking in NY - you will need to get a license through the State
Department of Education.  Some NJ jobs will also ask for NY license as
well.  Not knowing what your educational background is, it is something I
would get at as soon as possible to see what you may need.

The general website is www.op.nysed.gov

Good luck on your exam.

Vikki

On Tue, May 18, 2010 at 9:29 AM, Patsy Ruegg pru...@ihctech.net wrote:

 Jennifer,

 You should try some of the job placement agencies, they are always looking
 for techs to fill jobs and there is no cost to you.  I bet you will be
 contacted just from placing this post on Histonet.

 Patsy

 Patsy Ruegg, HT(ASCP)QIHC
 IHCtech
 12635 Montview Blvd. Ste.215
 Aurora, CO 80045
 720-859-4060
 fax 720-859-4110
 www.ihctech.net
 www.ihcrg.org


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
 Campbell
 Sent: Monday, May 17, 2010 2:13 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Moving across the country...

 Hi All,
  My husband and I will be moving from California to the east coast this
 late summer/early fall for his work.exciting but, a little scary!  I
 have started browsing possible histotech jobs available in either NY, CT
 or NJ but, its a rather daunting task when you're not familiar with the
 area or the labs out there!  And to add to the stress I am also
 currently studying for my HT certification which I will be taking in
 augustwhich I will hopefully pass so that I may have a little more
 luck job searching!  Does anyone have any advice for me as to where I
 could look into or know of any possibilities out there?
 Thanks for any advice you may have.

 Jennifer Campbell
 VDx Veterinary Diagnostics
 Davis, CA
 phone: (530) 753-4285
 fax: (530) 753-4286
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Re: [Histonet] anti 8 oxoguanine, 8 hydroxyguanine

[Victoria Baker]

Dr. Fabrice

Have you looked at the Trevigen website?  They have different tests that are
relevent to the work you are looking at.

Vikki Baker

On Tue, Apr 27, 2010 at 9:56 PM, Fabrice GANKAM gan...@googlemail.comwrote:


 Dear histonetters
 Will like to use the anti 8 hydroxyguanine (staining the oxidised DNA and
 RNA to asses for free radical damage to nucleic acids)for IHC but tried the
 antibody from abdserotec with dissapointing results.
 We tried it on liver slides but LOT of background.
 Could any one advise on other antibody or other protocol ?
 Thanks y'all

 Dr Fabrice GANKAM
 Intern



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[Histonet] Surgical veterinary reference manual

[Victoria Baker]

Hi

I need to locate a reference manual for veterinary surgical pathology - if
anyone knows of one would you please contact me?

Thanks

Vikki Baker
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[Histonet] metal embedding base molds

[Victoria Baker]

Hi-

I'm trying to locate embedding molds that Sakura/Tissue Tek makes.  All I'm
able to find at this point are the metal molds with the 'wings' on them.

Does anyone know a source?  With all the mergers etc it's been a little
challenging.

Thanks in advance

Vikki
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[Histonet] ehe tissue processor

[Victoria Baker]

Hi

Does anyone have experience with this type of tissue processor?  Any
feedback or information would be very appreciated.

Vikki Baker
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Re: [Histonet] c-erbB-2 clone 5A2

[Victoria Baker]

have you tried MONOSAN
www.monsan.com
Catalog number MONX10220

You can try doing Google/Google scholar as well.

Hope this is of some assistance.

Vikki

On Wed, Dec 17, 2008 at 2:38 PM, AMH-HistoLab amh-histo...@amh.org wrote:

 Our manufacturer (Nova Castra) no longer supplies c-erbB-2. .  Does anyone
  know where I can purchase this antibody. It needs to be clone 5A2. Thank
 You.

   Donna
   AMH Histolab
Abington,PA




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Re: [Histonet] RE: Recruiting Techs at work

[Victoria Baker]

Joelle -

I am very proud of you and so would Pat.

Vikki

On Mon, Dec 15, 2008 at 1:29 PM, joelle weaver joellewea...@hotmail.comwrote:


 Hi Terri
 I appreciated your post. I think all histotechs know that jobs are
 plentiful ( at least in the current market), and most of us get lots of
 postings- But, I really don't even take notice unless there is something
 that is making me think and wonder if the grass is greener- this only very
 rarely has to do with $ for me. And, I really couldn't speak for everyone
 out there, but above a certain dollar amount (needed to buy food and provide
 shelter) I have personally not been tempted to move to another lab unless
 other important aspects that I consider important, were missing from my work
 environment. I do understand that aggressive recruiters could be annoying to
 management trying to get and retain good techs. I know that managers
 struggle to get a decent wage for their best employees- I think that is
 admirable.
 However, maybe this will make some feel better? The main reasons that I
 have considered changing employers had to do with work schedule(
 unreasonable number of hours, all weekends/ no vacation etc), and not
 feeling valued as an employee-(a big one). Just to give an example of a
 moral killer- a lab policy states that ALL histotechs, regardless of
 training, education, or experience, are disqualified for any type of
 promotion within Histology-or anywhere in the lab. I think that is pretty
 crappy- and I think that kind of stuff really makes you feel de-valued-as a
 histotech,  even if you are not seeking advancement at the present time. It
 is just an opinion, but I think that if managers value their techs,and let
 them see that, they will keep the ones worth keeping!
 J. Weaver



  Date: Mon, 15 Dec 2008 11:00:37 -0500 From: tbr...@holyredeemer.com
 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recruiting
 Techs at work  I agree that if you have a great work environment with
 competitive pay your staff will stay put, but how many of us at the
 department supervisory/manager level can actually make the final decision
 concerning salary and pay scales for a position. I suspect that most of us,
 including myself, can only make well documented pleas for salary increases.
  When it comes to spending money, most institutions are reactive rather
 than proactive. If salaries are low, then you may have to lose a valuable
 tech before you can make the point that salaries need to be increased.  It
 may be a tough pill to swallow, but I am more than willing to let my folk
 listen to any pitchman that is trying to get their ear. Any tech worth
 his/her salt, knows that these calls are out there, so why try to hide it.
 If a tech can better themselves by a job change, then who am I to stand in
 their way. We generally discuss these in departmental meetings and I tell
 techs that I get frequent calls from recruiters and if they would like to
 correspond with them, may I give the recruiter their personal contact info.
 I ask in return, that if they do go elsewhere for a better salary, that they
 please give me the info, so I can use it to help increase the salary of
 those who remain. This keeps communication up front, honest, and open and I
 think in the long run, helps to quell murmuring about low salaries which are
 beyond my control as a supervisor. Just another viewpoint. Terri  Terri
 L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer
 Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046
 (215) 938-3676 phone (215) 938-3689 fax  Message: 18 Date: Sat, 13 Dec
 2008 13:46:57 + (UTC) From: lee2...@comcast.net Subject: Re:
 [Histonet] Offended  I think if you have a great work environment with
 competitve pay then you have nothing to worry about. Your staff will stay
 put.   
 -
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Re: [Histonet] problem with NSH website???

[Victoria Baker]

The office is closed for the holiday, as I have tried calling them.
I also tried to use the website over the holiday and had the same
issues that Chris van der Loos had.

I have faith - all will be restored by Monday afternoon!  ;-)
Hope everyone had a happy and festive Thanksgiving!

Vikki


On Fri, Nov 28, 2008 at 11:33 AM, Kristen Yaros [EMAIL PROTECTED] wrote:
 It was happening to me last night as well. I wonder if a server or something
 is down... somewhere.

 Kristen

 On Fri, Nov 28, 2008 at 8:31 AM, Pamela Marcum [EMAIL PROTECTED] wrote:



 I am not sure what is going on Chris.  I just tried it and I got the same
 result you did going to Isovers etc.  Obviously something is very wrong and
 they need to correct it.  I will forward you e-mail and my answer to them so
 they know it is not just a problem for Europe.  I assume they monitor
 HistoNet - I could be wrong.



 Pam Marcum


 - Original Message -
 From: C.M. van der Loos [EMAIL PROTECTED]
 To: histonet netserver histonet@lists.utsouthwestern.edu
 Sent: Friday, November 28, 2008 4:02:21 AM GMT -05:00 US/Canada Eastern
 Subject: [Histonet] problem with NSH website???

 Dear All,Desperately I tried to submit my abstracts for the NSH convention.
 Using the links for submission in the email we got from Aubrey Wanner/Kim
 Simmons early November, the browser goes to Isovera.com a domain for sale in
 Germany, provided by Sedo Domain Parking. Obviously something that has
 nothing to do with NSH. The same problems occurs at the NSH website. You can
 go to the NSH website as normal, but clicking on 'meetings/event' and
 selecting 'symposium/convention' one is re-directed to that same crap. What
 is going on here??? Does this problem also occurs with others???Chris van
 der Loos, PhD
 Dept. of Pathology
 Academic Medical Center M2-230
 Meibergdreef 9
 NL-1105 AZ Amsterdam
 The Netherlands
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Re: [Histonet] Who inspects research labs?

[Victoria Baker]

Hi Emily,

so you are not inspected by CAP then?

Vikki Baker

On Mon, Nov 17, 2008 at 1:55 PM, Emily Sours [EMAIL PROTECTED] wrote:
 If you're at a university, both IACUC and Environmental Health and Safety
 will.
 Outside of academics, I have no idea.

 Emily
 --
 Millions of creatures walk the earth
 Unseen, both when we wake and when we sleep.
 -John Milton
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