Re: [Histonet] Who can perform high complexity testing

[Walter Benton via Histonet]

stopathology, meet the qualifications of § 493.1449 (b) or (l) to 
perform tissue examinations.

[ 57 FR 7172, Feb. 28, 1992, as amended at 58 FR 5236, Jan. 19, 1993; 58 FR 
39155, July 22, 1993; 60 FR 20050, Apr. 24, 1995]


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
410-768-5961 (Lab)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.


-Original Message-
From: Amy Johnson via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, August 03, 2017 2:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Who can perform high complexity testing

What kind of credentials does one need to perform High Complexity testing?  My 
understanding was if you had a 4 year degree of some kind (with some 
science/biology background) you are qualified to perform these types of tests.  
Others have said if you don't have a degree but have your certification as a HT 
or MLT you are qualified as well

Thanks for clarifying,

Amylin Johnson, B.S. HTL(ASCP)
Associates in Pathology
Wausau Wi 54401
715-847-2130

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Re: [Histonet] slides/cassettes printers points of view

[Walter Benton via Histonet]

We have the original Slidemates (not the newer AS model) and Printmate. Both 
instruments work well.

The slidemate used a thermal ribbons to print. Certain slide coatings (in the 
area where you write/etch/print) are better suited for thermal print 
technology. Not sure what type of slides you use now, but you consider that in 
your purchase decision. In addition slidemates have a small footprint.

Printmate also has a thermal ribbon and you can print 2D barcodes and other 
pertinent identifiers on the cassettes. Generally speaking the printer works 
well with minimal down time due to failures.

Feel free to reach out if you additional questions.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
410-768-5961 (Lab)
Chesapeakeurology.com
Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.




-Original Message-
From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, July 28, 2017 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] slides/cassettes printers points of view

Hello Histonettes!!!
I was wondering if somebody are using PRIMERA cassettes and slides printers 
and/or SLIDEMATE slide printer and PRINTMATE AS cassette printer From Thermo.
I am undecided which one to buy. I really love both. They have a lot of 
similarities in efficiency, price and quality BUT my manager is being hearing 
that primera works with cut corner slides ONLY otherwise will create a lot of 
problems.
Have not hear too much about slidemate yet.
If anybody can share their experiences with those printers that can help me to 
decide, I will appreciate. :)



UT Southwestern


Medical Center



The future of medicine, today.

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Re: [Histonet] Section adherence issues (IHC)

[Walter Benton via Histonet]

Thanks. This is helpful information.

-Original Message-
From: Hujet, Matthew [mailto:matthew.hu...@ssmhealth.com]
Sent: Wednesday, July 12, 2017 1:35 PM
To: Walter Benton <wben...@cua.md>; Greg Dobbin <greg.dob...@gmail.com>
Cc: kgevele...@ihis.org; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Section adherence issues (IHC)

We have had really good luck with both the TOMO and Millennia 2000 adhesion 
slides. We initially tried using the TOMO slides for everything (IHC, special 
stains, and H's), but found that there was too much background staining on 
the specials and H's. For IHC, the TOMO slides were great. We then switched 
to the Millennia 2000 for everything, and they have been working really well 
across the board. With regards to just IHC, I personally feel that the TOMO 
slides are better, but if you only want one type of glass in your lab, the 
Millennia 2000 are the way to go.

Matthew Hujet
Histology Technical Specialist
SSM Health St. Mary's Hospital - Madison
700 S. Park St.
Madison, WI 53715

Please note my new email address: matthew.hu...@ssmhealth.com



-Original Message-
From: Walter Benton [mailto:wben...@cua.md]
Sent: Wednesday, July 12, 2017 10:34 AM
To: Greg Dobbin <greg.dob...@gmail.com>
Cc: kgevele...@ihis.org; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Section adherence issues (IHC)

Greg,

We are running into a similar issue on the Biocare Nemesis platform and the 
support there suggest humidity to be the issue. Our sections stay adhered to 
the slides through depar, antigen retrieval, but appear to fall off during the 
staining process. It is not consistent. We are going to try some other slides 
and see if the issue persists. I'd be interested to hear your findings and 
resolution.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061




-Original Message-
From: Greg Dobbin via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, July 12, 2017 11:09 AM
To: histonet@lists.utsouthwestern.edu
Cc: kgevele...@ihis.org
Subject: [Histonet] Section adherence issues (IHC)

Hi Folks,
I haven't been on here much lately but it is nice to know that we are all here 
for each other when needed!

My problem is section adherence during IHC staining. And it is not all of the 
time it is intermittent; so not all of the time and not on all slides when it 
does occur.

Background:
We have a Bond-III immunostainer and we use the Leica Apex charged slides for 
our IHC stains. We have no additives in our water baths. Our sections drain in 
a stand and then any trapped water under the section is either flicked or 
wicked away as needed prior to placing the slides in a rack in a
60 C oven for 30 mins prior to staining.  We do not do on board baking 
(primarily because on board baking is only 10 mins long and with the section 
lifting issue we want longer).

Some of the specimen types are more susceptible it would seem. Cervix LEEP 
specimens tend to be bad, we use a 4mm punch to obtain some of our control 
tissue and so the breast tissue we use for Myosin Heavy Chain seems to be a bad 
one and sometimes our ER/PR control sections (but not always). Fine needle 
cores where a lot of tumour is present tend to be bad (again not always).

We have tried baking longer, we turn ourselves inside out trying to get all of 
the water out from under the sections, we have tried charged slides from 
another manufacturer. I have looked at the HIER protocols and none are 
extraordinary in nature. We use very clean covertiles (no scratches or 
blemishes). Our specimens are fixed for 24hrs before processing. We use the 
same slides for Special Stains and don't have this issue there.

I need some new suggestions to try! All ideas welcome.
Cheers,
Greg

--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!* 
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the sole use of the in

Re: [Histonet] Section adherence issues (IHC)

[Walter Benton via Histonet]

Greg,

We are running into a similar issue on the Biocare Nemesis platform and the 
support there suggest humidity to be the issue. Our sections stay adhered to 
the slides through depar, antigen retrieval, but appear to fall off during the 
staining process. It is not consistent. We are going to try some other slides 
and see if the issue persists. I'd be interested to hear your findings and 
resolution.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061




-Original Message-
From: Greg Dobbin via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, July 12, 2017 11:09 AM
To: histonet@lists.utsouthwestern.edu
Cc: kgevele...@ihis.org
Subject: [Histonet] Section adherence issues (IHC)

Hi Folks,
I haven't been on here much lately but it is nice to know that we are all here 
for each other when needed!

My problem is section adherence during IHC staining. And it is not all of the 
time it is intermittent; so not all of the time and not on all slides when it 
does occur.

Background:
We have a Bond-III immunostainer and we use the Leica Apex charged slides for 
our IHC stains. We have no additives in our water baths. Our sections drain in 
a stand and then any trapped water under the section is either flicked or 
wicked away as needed prior to placing the slides in a rack in a
60 C oven for 30 mins prior to staining.  We do not do on board baking 
(primarily because on board baking is only 10 mins long and with the section 
lifting issue we want longer).

Some of the specimen types are more susceptible it would seem. Cervix LEEP 
specimens tend to be bad, we use a 4mm punch to obtain some of our control 
tissue and so the breast tissue we use for Myosin Heavy Chain seems to be a bad 
one and sometimes our ER/PR control sections (but not always). Fine needle 
cores where a lot of tumour is present tend to be bad (again not always).

We have tried baking longer, we turn ourselves inside out trying to get all of 
the water out from under the sections, we have tried charged slides from 
another manufacturer. I have looked at the HIER protocols and none are 
extraordinary in nature. We use very clean covertiles (no scratches or 
blemishes). Our specimens are fixed for 24hrs before processing. We use the 
same slides for Special Stains and don't have this issue there.

I need some new suggestions to try! All ideas welcome.
Cheers,
Greg

--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!*
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Re: [Histonet] scrubs/business casual

[Walter Benton via Histonet]

Bill,

It is a required uniform for our staff. First three sets are provided upon 
hire. All additional scrubs are purchased by the employee.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates



-Original Message-
From: O'Donnell, Bill via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, June 21, 2017 9:36 AM
To: Histonet (histonet@lists.utsouthwestern.edu) 
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] scrubs/business casual

Just a little poll.

How many histology labs allow scrubs?

Of those that are allowed scrubs, do you purchase your own, or are they 
provided?

Bill O'Donnell
Good Sameritan Hospital
Histology

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Re: [Histonet] Microwave processors

[Walter Benton via Histonet]

Charles,

Milestone Medical http://www.milestonemed.com/

The processors are great. Easy to operate and use minimal chemicals based on 
the protocols you select.  H and IHC are very good.

Check out the various product offerings to see which unit will meet your needs 
best.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, June 14, 2017 9:39 AM
To: Histo List <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Microwave processors

Does anyone have any suggestions for microwave processing units? My lab 
processes about 110 blocks max a day from GI biopsies to breast tissue on size

Sent from my iPhone
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Re: [Histonet] CAP code for H

[Walter Benton via Histonet]

Hope this helps.

ANP.10042 Histologic Prep Quality Phase I
There is a written procedure that describes the process by which pathologists 
or their
designees provide feedback to the histology laboratory on the quality of 
histologic
preparations. This procedure must include the daily recording of the quality of 
the
histologic preparations for each day of tissue processing and slide preparation.
NOTE: Histologic preparations refer to H & E sections, histochemical stains,
immunohistochemistry preparations, and in situ hybridization preparations.
This requirement applies to laboratories that process and interpret histologic 
preparations at the
same location, as well as laboratories that interpret histologic preparations 
processed at another
laboratory (regardless of that outside laboratory's accrediting organization).
Records of such feedback and corrective action taken when problems are 
identified may be
incorporated into the laboratory's quality management program.
Specific quality control requirements for special stains, immunohistochemistry, 
and other special
studies are found elsewhere in this checklist.
Evidence of Compliance:
✓ Records of feedback and corrective action for problems identified with 
histologic prep quality

ANP.11734 Slide Quality Phase II
Slides are of sufficient quality for diagnosis.
NOTE: Histopathology slides must be of adequate technical quality to be 
diagnostically useful.
Criteria to evaluate include adequate tissue fixation, processing, thickness of 
sections, absence
of interfering tissue folds and tears, and good staining technique and cover 
slipping. For
hematoxylin and eosin and other routine stains, the patient slide serves as the 
internal control to
ensure adequate staining technique. The sections must be cut from sufficient 
depth in the block
to include the entire tissue plane.

**REVISED** 07/28/2015
CYP.04300 Daily QC Phase II
There are records of daily review of the technical quality of cytologic 
preparations by the
pathologist or supervisory-level cytotechnologist.
NOTE: The technical quality of cytologic preparations must be checked daily (on 
days processing
occurs). This includes checking all stains for predicted staining 
characteristics each day of use.
This check must include all of the types of preparations seen that day such as 
cytospins, cell
blocks, and liquid based preparations.
If preparation and staining is performed by a different laboratory, there must 
be a procedure for
the laboratory performing the preparation and staining to verify the 
acceptability of the quality
of preparations and the acceptability of controls (if needed) before transfer. 
Records of this
verification must be readily available to the laboratory performing 
interpretations. There should
also be a mechanism for feedback from the interpreting laboratory to the 
laboratory that prepared
the slides of any issues with the preparations.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.


-Original Message-
From: Nina J. Rich via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, April 24, 2017 11:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP code for H

Does anyone know if CAP requires that labs run an H control daily? If so what 
is the # for that requirement?  Also, for pap stains?

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Re: [Histonet] looking for a slider printer

[Walter Benton via Histonet]

http://accuplace.com/products/pslim/

Manufacturers of the original slidemate (Thermo Fisher).

Solid instruments with minimal fuss. Keep them clean and maintained according 
to manufacturer specs and they work well.

Using proper Thermal Slides are also important for getting a good print and not 
destroying the print head.

Feel free to reach out if you have additional questions.

Walter Benton HT(ASCP)QIHC


-Original Message-
From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, April 21, 2017 12:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] looking for a slider printer

Dear Histonets!!!
We are looking to buy a slide printer for a low cost, durable and easy to work. 
W are a very small lab, we don't' need anything fancy. If you know somebody or 
have a good experience with one please let me know.


Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: blanca.lo...@utsouthwestern.edu




UT Southwestern


Medical Center



The future of medicine, today.

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Re: [Histonet] Survey!!!!!!

[Walter Benton via Histonet]

​Patti,


If you are able to incorporate the special stains along with the H on the 
automated stainer, I think that would be the best option. Based on your 
information you may have 120-180 slides to coverslip by hand each day.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Patti Nelson - PNP Lab Consultant <nelsonr...@verizon.net>
Sent: Friday, March 31, 2017 3:50 PM
To: Walter Benton; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Survey!!

Walter the staff consist of 1 Histotech and 1 lab assistant. On an average 
there will be 2 special stains per block. Hope that helps.

Sincerely,

PATTI NELSON  H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR DGC/ZADEH LABS
PO BOX 412
CABAZON, CA. 92230
909-841-9761
nelsonr...@verizon.net<mailto:nelsonr...@verizon.net>
CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti 
Nelson, PNP Laboratory Consultants and are intended only for the addressee. The 
information contained in this message is confidential and may contain 
privileged, confidential, proprietary and/or exemption from disclosure under 
applicable law.  Unauthorized forwarding, printing, copying, distribution, or 
use of such information is strictly prohibited and may be unlawful.  If you are 
not the addressee, please promptly delete this message and notify the sender 
ofthe delivery error by e-mail or you may call  909-841-9761.


-Original Message-
From: Walter Benton <wben...@cua.md>
To: Patti Nelson - PNP Lab Consultant <nelsonr...@verizon.net>; Histonet 
<histonet@lists.utsouthwestern.edu>
Sent: Fri, Mar 31, 2017 12:39 pm
Subject: Re: [Histonet] Survey!!

How many slides are you producing from that number of blocks? How many techs do 
you have that are able to hand stain and/or hand coverslip?

Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Patti Nelson - PNP Lab Consultant via Histonet 
<histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>>
Sent: Friday, March 31, 2017 2:58 PM
To: Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Survey!!

Hi Everyone,

I just wanted to get everyone's opinion. If you had to chose between buying a 
Auto Side Stainer or Auto Slide Cover Slipper, which one would you chose? Lets 
say your volume was around 60 to 80 blocks a day and you worked for a GI Lab. 
Everyone's input would be greatly appreciated.



Sincerely,

PATTI NELSON H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR DGC/ZADEH LABS
PO BOX 412
CABAZON, CA. 92230
909-841-9761
nelsonr...@verizon.net<mailto:nelsonr...@verizon.net>
CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti 
Nelson, PNP Laboratory Consultants and are intended only for the addressee. The 
information contained in this message is confidential and may contain 
privileged, confidential, proprietary and/or exemption from disclosure under 
applicable law. Unauthorized forwarding, printing, copying, distribution, or 
use of such information is strictly prohibited and may be unlawful. If you are 
not the addressee, please promptly delete this message and notify the sender 
ofthe delivery error by e-mail or you may call 909-841-9761.

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disclosure

Re: [Histonet] Survey!!!!!!

[Walter Benton via Histonet]

How many slides are you producing from that number of blocks? How many techs do 
you have that are able to hand stain and/or hand coverslip?

Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Patti Nelson - PNP Lab Consultant via Histonet 
<histonet@lists.utsouthwestern.edu>
Sent: Friday, March 31, 2017 2:58 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Survey!!

Hi Everyone,

I just wanted to get everyone's opinion. If you had to chose between buying a 
Auto Side Stainer or Auto Slide Cover Slipper, which one would you chose? Lets 
say your volume was around 60 to 80 blocks a day and you worked for a GI Lab. 
Everyone's input would be greatly appreciated.



Sincerely,

PATTI NELSON  H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR DGC/ZADEH LABS
PO BOX 412
CABAZON, CA. 92230
909-841-9761
nelsonr...@verizon.net
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Re: [Histonet] Newbe help

[Walter Benton via Histonet]

Start with this link and navigate through the website. There are various online 
and in-person programs. Best of luck.
http://nsh.org/content/schools


-Original Message-
From: Keith Brown via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, March 06, 2017 11:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Newbe help

Hi.
I've looked through the archives of this group to little avail. If i missed an 
answer to my questions that was previously posted to this list, my apologies.

I'm looking to get into the histotech field, but I'm not sure how to go about 
it. I'm changing careers after 20 years as a journalist. I have an associates 
and a bachelor's degree, neither of which are in anything remotely science-y: 
Liberal Arts and Journalism, respectively.

I live in New Jersey, where there does not appear to be any histology training 
programs, yet most of the jobs I see posted throughout the state want a 
certification.

I have a friend in Seattle who fell into histology after journalism, but he 
admits he got lucky and still does not have a certification. He gets paid 
slightly less, he says, but not by much. It sounds like a good career choice 
for me, but I'm not sure how to get it started.

Any and all advice is appreciated.

Thanks,

Keith.
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Re: [Histonet] hand staining immunos

[Walter Benton via Histonet]

http://www.agilent.com/cs/library/technicaloverviews/public/08002_ihc_staining_methods.pdf


-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, February 09, 2017 10:51 AM
To: Jennifer ; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] hand staining immunos

You do not have to "babysit" the procedure for it has well defined/timed steps. 
You just need a timer and check the slides when required. As to procedural "dos 
& donts" try to get a copy of the DAKO IHC manual.René

On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet 
 wrote:


 Hello,



My dermatopathologist wants me to start hand staining immunos  for MITF. I 
would really like some feedback about everyone's experiences/thoughts with 
manually hand staining immunos and especially with MITF. Pros and cons? I am 
concerned about the amount of time this will take for a tech to babysit the 
stain and also about the consistency of the quality in the end result.  Your 
opinions are much appreciated.



Thank you!



Jennifer

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Re: [Histonet] Syringe for Lab Vision 720D Stainer Needed!

[Walter Benton via Histonet]

Check Biocare Medical. They use the same "box" and just place their name on it. 
Biocare calls it the Nemesis.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Sandra Cheasty via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, January 30, 2017 4:01 PM
To: Histonet (histonet@lists.utsouthwestern.edu) 
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Syringe for Lab Vision 720D Stainer Needed!

Hello everyone!
Can someone tell me where I can get a replacement syringe for 
the Lab Vision 720 stainer? It is supposed to be part # NM014. Thermo Fisher 
has been unable to help me so far.
Thanks!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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Re: [Histonet] Slide and Block Storage

[Walter Benton via Histonet]

Depending on the contract that you select with your vendor, you can determine a 
cutoff time for same day deliveries. We order by noon and have the delivery by 
3pm the same day. Orders placed after 12pm are delivered on the next business 
morning.

I'm sure there are more rapid options, but they are probably more costly as 
well.

-Original Message-
From: Amy Self via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, January 12, 2017 10:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide and Block Storage

Happy Thursday connected to Friday,

Have a few questions about slide and block storage...


Are you blocks and slides stored on-site or off-site at a record control type 
facility?

If your blocks and slides are stored off-site how do you get the material that 
you need at any given point of the day?



Thanks in advance for your help,
Amy Self
Histology Lab Senior Tech
Lab
Tidelands Georgetown Memorial Hospital
606 Black River Road
Georgetown, SC 29440
843-520-8711
as...@tidelandshealth.org

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Re: [Histonet] CAP checklist help

[Walter Benton via Histonet]

Leica sells a temperature verification kit that should meet your needs for CAP 
compliance and documentation. We use it for our Leica Thermobrites used in the 
processing of FISH.

-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, December 28, 2016 11:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP checklist help

I need help with figuring out how to meet checklist # ANP.23420.   We use
the Leica Bond III and MAX  to do our ISH slides.  How do I test the 
temperature and how would you recommend recording the results?

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] Temp Assignment for Chesapeake Urology

[Walter Benton via Histonet]

To all:

Our lab is in search of temp help to cover an extended absence by a staff 
member.

The ideal candidate will have Prostate Grossing experience and be eligible to 
gross based on CLIA 88 Guidelines. In addition, this person should be a strong 
prostate biopsy cutter and embedder. The assignment will be 4 weeks in length. 
This will be a 40hr/week assignment. The shift may start as early as 4 am or 
6am depending on the individuals skills and abilities to meet the needs of the 
lab.

If interested, please send your resume along with desired salary to:
mhw...@cua.md<mailto:mhw...@cua.md> and myself 
wben...@cua.md<mailto:wben...@cua.md>


From: Walter Benton
Sent: Monday, December 19, 2016 12:07 PM
To: histonet@lists.utsouthwestern.edu
Cc: Michael Hwang <mhw...@cua.md>
Subject: Temp Assignment for Chesapeake Urology

To all:

Our lab is in search of temp help to cover an extended absence by a staff 
member.

The ideal candidate will have Prostate Grossing experience and be eligible to 
gross based on CLIA 88 Guidelines. In addition, this person should be a strong 
prostate biopsy cutter and embedder. The assignment will be 4 weeks in length. 
This will be a 40hr/week assignment. The shift may start as early as 4 am or 
6am depending on the individuals skills and abilities to meet the needs of the 
lab.

If interested, please send your resume along with desired salary to:
mhw...@cua.md<mailto:mhw...@cua.md> and myself 
wben...@cua.md<mailto:wben...@cua.md>

Thanks

Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
Chesapeakeurology.com<https://www.cua.md/owa/chesapeakeurology.com>

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

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Re: [Histonet] specimen submission pads

[Walter Benton via Histonet]

Biopsy wraps or 
http://www.amazon.com/Graham-Jumbo-Papers-Perms-1000ct/dp/B001J9R93W

Either of these items can be wrapped to form an envelope preventing the tissue 
from floating around.

-Original Message-
From: Linda Margraf via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, April 06, 2016 12:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] specimen submission pads

Hi Histonetters,
We are having trouble in the Gross room,  removing small specimens from the 
pads/gauze that OR personnel put them on which they then immerse in formalin.  
We thought the gauze was challenging to remove tiny specimens from but have 
found that the Telfa pads the OR is now using come apart in the fixative and 
are even more challenging to work with. The GI lab uses sponges but they would 
be too small for the usual size containers the OR send us.  Any suggestions?

Thanks in advance,
Linda M
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[Histonet] Brain IHC

[Walter Benton via Histonet]

Please see message below. Feel free to reach out directly to the Pathologist.

From: Rudolph J Castellani [mailto:rudolph.castell...@med.wmich.edu]
Sent: Friday, March 25, 2016 8:55 AM
To: Walter Benton <wben...@cua.md>
Subject:


Another question - is there any option of embedding big brain slices in 
paraffin and doing 50 micron IHC on them, or is that not possible?


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Re: [Histonet] Sliding Microtome

[Walter Benton via Histonet]

Thanks to those who have responded.

Anyone able to share or train on techniques for free floating IHC? He needs 
that skill set too.

-Original Message-
From: Walter Benton via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, March 24, 2016 8:45 PM
To: Histonet <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Fw: Sliding Microtome

(2nd attempt)?

To all:



I have a Neuro-Pathologist colleague that is looking to learn how to use a 
sliding microtome. Is there anyone out there that would be willing to show him 
the basics?



Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Walter Benton
Sent: Thursday, March 24, 2016 10:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: Sliding Microtome

To all:

I have a Neuro-Pathologist colleague that is looking to learn how to use a 
sliding microtome. Is there anyone out there that would be willing to show him 
the basics?



Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com<https://www.cua.md/owa/chesapeakeurology.com>

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

CONFIDENTIALITY NOTICE: The information contained in this electronic message is 
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[Histonet] Fw: Sliding Microtome

[Walter Benton via Histonet]

(2nd attempt)?

To all:



I have a Neuro-Pathologist colleague that is looking to learn how to use a 
sliding microtome. Is there anyone out there that would be willing to show him 
the basics?



Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Walter Benton
Sent: Thursday, March 24, 2016 10:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: Sliding Microtome

To all:

I have a Neuro-Pathologist colleague that is looking to learn how to use a 
sliding microtome. Is there anyone out there that would be willing to show him 
the basics?



Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com<https://www.cua.md/owa/chesapeakeurology.com>

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

CONFIDENTIALITY NOTICE: The information contained in this electronic message is 
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Re: [Histonet] Plastic coverslip Loosening

[Walter Benton via Histonet]

This may work. I have used a version of this product on glass coverslips and 
slides to transfer paraffin sections.

http://www.newcomersupply.com/product/mount-quick


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.




-Original Message-
From: Helen Fedor via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, March 22, 2016 9:45 AM
To: HISTONET (histonet@lists.utsouthwestern.edu) 
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Plastic coverslip Loosening

Hello, I did try and search the archives to see if there was any advice on this 
but didn't find an answer.

Do you know of a good way to fix H slides that had plastic coverslips where 
the coverslip is lifting from the slide and in some cases coming completely 
off? In all cases the tissue is on the coverslip.

Thanks in advance.



Helen L. Fedor


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Re: [Histonet] Microscopic slides

[Walter Benton via Histonet]

Try Globe and Tanner slides, both should work well in your slidemate.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Vickroy, James via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, March 07, 2016 1:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microscopic slides


We are once again reviewing the microscopic slides we use.  We have found the 
sldies we have used on a regular basis take too long to dry properly which 
gives us some undesireable artifacts.  I have longed for a "one size fits all" 
microscopic slide.One of the slides I used in the past is not possible 
given that I have the Thermofisher Slidemates.   The slidemates require a very 
smooth labeled end.   Recently we tested several slides both hydrophobic and 
hydrophilic.  We really liked a hydrophilic slide by Leica but am told it 
doesn't work well for IHC's and special stains.   Our current slide is a 
"hydrophobic" slide which traps water easily underneath the section and 
therefore we having drying problems.  If we don't dry them for at least twenty 
minutes we get nuclear bubbling.  Leica has another slide we tried that is 
"hydrophobic" but with "hydrophilic" tendencies.  My staff thought tried that 
slide and found them to be very similar to the "hydrophobic" slide we already 
have.

We have tried the Stat-lab M2000 and they seem to  dry better but there often 
is a "trick" to getting the sections to stick to the slide without sliding off 
of the end.Of course we might just have to use two different slides, one 
for our routine H's and one for the IHC's and Special stains.   Anybody have 
any thoughts on this?

And is a "hydrophobic slide" that has "hydrophilic tendencies" really possible?

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com>



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are hereby notified that any dissemination, distribution or copying of this 
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Re: [Histonet] Giemsa Tissue control options

[Walter Benton via Histonet]

http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MGIEMSA.PDF


-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, February 23, 2016 11:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Giemsa Tissue control options

I need to run a giemsa test and haven't done it in a long time. Is there 
something I can use as a control tissue other than a blood smear or spleen?

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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Re: [Histonet] Need Slide Cabinets

[Walter Benton via Histonet]

http://www.phoenixmetalproducts.com/


-Original Message-
From: Rayen Gonzalez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, February 12, 2016 12:05 PM
To: histonet@lists.utsouthwestern.edu
Cc: Lin Bustamante 
Subject: [Histonet] Need Slide Cabinets

Hello all!

Our lab is in need for STACKABLE METAL SLIDE CABINETS. I would like to know 
which vendors supply this product and if you can send me a quote? Or if your 
lab is content with the slide cabinets you use, if you can give me your vendors 
information?


Thank you,


Rayen Gonzalez B.S., HT (ASCP)
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Re: [Histonet] NSH Continue education award

[Walter Benton via Histonet]

http://www.nsh.org/content/scholarships


-Original Message-
From: Bustamante, Lin via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 10, 2016 12:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] NSH Continue education award

I would like to nominate a student I have. Can someone guide me what to do next?
Thank you very much.
Lin.

Lin S. Bustamante B.Sc. H.T.(ASCP)
Research Associate
Texas A University
College of Veterinay Medicine
VIBS Histology Laboratory Supervisor
Room 107 VMA
College Station, Texas 77843-4458
(979)845-3177
(979)458-3499 Fax

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Re: [Histonet] Temperatures

[Walter Benton via Histonet]

Secondary thermometer in paraffin reservoir of  embedding center.

-Original Message-
From: Fawn Bomar via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 03, 2016 11:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperatures

Hi All,



I was wondering how everyone tracked their cryostat and paraffin temperatures.  
Do you all just use the machine temperature, or do you all use the machine and 
a second thermometer as verification?



Thank you

Fawn
-
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Re: [Histonet] tissue flotation bath

[Walter Benton via Histonet]

http://www.sakura.eu/Our-products/item/8/Microtomy/31/Sakura-Water-Bath-Hot-Plate/Downloads

It appears to have a light, but can't tell for sure.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Sanders, Jeanine (CDC/OID/NCEZID) via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, February 02, 2016 11:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] tissue flotation bath

Hello everyone,

I have a Surgipath flotation bath that I love. It is square, black inside, 
lighted and no glass liner. The things heats up in 5 minutes!

I know Surgipath is no longer, but I thought I found a similar product online a 
year or so ago but now cannot find anything like it. (Except for 1 but has no 
light)

Can anyone direct me? And vendors are more than welcome to reply.

Thank you,

Jeanine H. Sanders, BS, HT (ASCP), QIHC
Centers for Disease Control and Prevention
1600 Clifton Rd., NE MS/G-32
Atlanta, GA 30329
j...@cdc.gov<mailto:j...@cdc.gov>
404-639-3590

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Re: [Histonet] Tissue processing question

[Walter Benton via Histonet]

We use hair wrapping paper used for perms. It is the same paper called "biopsy 
wraps," but at a significant price reduction. You can buy a variety of sizes 
and the wraps do not cause artifacts and are porous enough for ample solution 
penetration. Biopsy paper comes in blue and other colors, but the hair wraps 
only come in white. Our overall experience with them has been great.

Let me know if you need any other information.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, January 29, 2016 12:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue processing question

Hello all,

 I was wondering what everyone uses to secure biopsy and scant tissues through 
processing. Also what would you recommend placing breast cores in for 
processing. Having an argument with grossing staff and pathologist about 
whether to use sponges, tissue paper, or something else. Looking for the best 
option that will allow for reagents to penetrate tissue and not leave any 
artifact

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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Re: [Histonet] Tissue processing question

[Walter Benton via Histonet]

Histoscreen cassettes will work as well. Generally the cassette options are 
expensive and may not work in all cassette printers, if you are using one.

http://www.thermoscientific.com/content/tfs/en/product/tissue-loc-histoscreen-cassettes.html

Ultimately, get samples of whatever you like to use.

From: Caroline Miller [mailto:mi...@3scan.com]
Sent: Friday, January 29, 2016 1:36 PM
To: Walter Benton <wben...@cua.md>
Cc: Charles Riley <cri...@dpspa.com>; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue processing question

I really like this type:
https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tissue-cassettes-micromesh-chamber-8/p-2782584
(although I buy them from mastertech, but they seem to have dissapeared from 
their website)
They are great for both large tissues, and also biopsies. A long time ago when 
I worked in a clinical lab we used the tissue paper and I found that if 
everything was not heated just right the biopsies would stick and things like 
currettes were hard to scrape up from there, I always thought I was doing the 
tissue damage
yours
mills

On Fri, Jan 29, 2016 at 10:06 AM, Walter Benton via Histonet 
<histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> 
wrote:
We use hair wrapping paper used for perms. It is the same paper called "biopsy 
wraps," but at a significant price reduction. You can buy a variety of sizes 
and the wraps do not cause artifacts and are porous enough for ample solution 
penetration. Biopsy paper comes in blue and other colors, but the hair wraps 
only come in white. Our overall experience with them has been great.

Let me know if you need any other information.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Charles Riley via Histonet 
[mailto:histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>]
Sent: Friday, January 29, 2016 12:43 PM
To: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Tissue processing question

Hello all,

 I was wondering what everyone uses to secure biopsy and scant tissues through 
processing. Also what would you recommend placing breast cores in for 
processing. Having an argument with grossing staff and pathologist about 
whether to use sponges, tissue paper, or something else. Looking for the best 
option that will allow for reagents to penetrate tissue and not leave any 
artifact

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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without making a copy.

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--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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Re: [Histonet] Tissue processing question

[Walter Benton via Histonet]

We use hair wrapping paper used for perms. It is the same paper called "biopsy 
wraps," but at a significant price reduction. You can buy a variety of sizes 
and the wraps do not cause artifacts and are porous enough for ample solution 
penetration. Biopsy paper comes in blue and other colors, but the hair wraps 
only come in white. Our overall experience with them has been great.

Let me know if you need any other information.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, January 29, 2016 12:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue processing question

Hello all,

 I was wondering what everyone uses to secure biopsy and scant tissues through 
processing. Also what would you recommend placing breast cores in for 
processing. Having an argument with grossing staff and pathologist about 
whether to use sponges, tissue paper, or something else. Looking for the best 
option that will allow for reagents to penetrate tissue and not leave any 
artifact

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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Re: [Histonet] Disposal of blocks and slides

[Walter Benton via Histonet]

Charles,

Not sure if you are CAP accredited or will be, but this is from our checklist 
for the last inspection period.

ANP.12500 Record Retention Phase II
Surgical pathology records and materials are retained for an appropriate period.
NOTE 1: Minimum requirements for surgical pathology, providing these are not 
less stringent
than local, state and national regulations, are:
1. Accession log records - 2 years
2. Wet tissue (stock bottle) - 2 weeks after final report
3. Paraffin blocks - 10 years (subject to Notes 2 and 3, below)
4. Glass slides (including control slides) and reports - 10 years (slides must 
remain
readable for this period)
5. Surgical pathology reports - 10 years (see Notes 4 and 5, below)
6. Fluorochrome-stained slides - at the discretion of the laboratory director
7. Fine needle aspiration slides - 10 years
8. Images of FISH studies - 10 years (see Note 6, below)
There must be a documented policy for protecting and preserving the integrity 
and retrieval
of surgical pathology materials and records. The retention period should be 
extended, when
appropriate, to provide documentation for adequate quality control and medical 
care.

NOTE 2: Regarding extra-institutional release of blocks for research purposes: 
Federal
regulations require that a laboratory retain paraffin blocks for two years 
unless the tissue is
blocked specifically for research and not used for patient diagnostic 
purposes.* The CAP
Commission on Laboratory Accreditation (CLA) requires, however, that paraffin 
blocks used for
patient diagnostic purposes must be kept for at least 10 years. Nevertheless, 
such blocks may
be released for research purposes after the two-year regulatory requirement if 
all of the following
criteria are met:
1. For laboratories subject to US regulations, formal written authorization is 
obtained
in accordance with the requirements of HIPAA if identifiable patient 
information is
released.
2. The laboratory retains sufficient blocks to support the diagnosis for the 
full 10-year
period.
3. Provision is made for retrieval by the laboratory of any blocks or material 
that remain
after use in research, if the blocks or material are needed for diagnostic, 
legal, or
other legitimate purposes.
4. The laboratory meets other relevant requirements including but not limited 
to the
requirements of the institution, the directives of any applicable institutional 
review
board (IRB) or similar entity; and state and local laws and regulations.
NOTE 3: Given that patient survival rates are increasing and the continued 
emergence of
treatment based on biomarker testing, which at times may be required on the 
original tissue, it
is recommended that, whenever feasible, tissue block retention from patients 
with diagnosed
malignancies be retained beyond the 10 year requirement.
NOTE 4: Pathology reports may be retained in either paper or electronic format. 
If retained
in electronic format alone, however, the electronic reports must include a 
secure pathologist
electronic signature. Images of paper reports--such as microfiche or PDF 
files--are acceptable.
NOTE 5: Reports of outside consultations performed on cases from the laboratory 
(whether or
not such consultation was requested by the laboratory) must be retained for 10 
years after the
date on which the original report was issued.
NOTE 6: There is no retention requirement for images when the source slides 
remain readable
for the required 10-year retention period. The 10-year retention requirement 
applies to images of
slide preparations that are not readable for the 10-year period (e.g. FISH 
studies).
*The restriction on release of blocks does not prohibit release of blocks for 
purposes of
treatment, diagnosis, prognosis, etc., for patients on research protocols as 
long as release
is consistent with patient privacy regulations (e.g. HIPAA) and applicable 
state and local


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, January 12, 2016 8:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Disposal of blocks and slides

Hello all,

 What are the guidelines for disposal of blocks and slides? This was never 
discussed in my program and I am now in charge of the department. No one who 
currently works here has been through the process. Any help will greatly be 
appreciated.

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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Re: [Histonet] NSH Financial and Tax Statements

[Walter Benton via Histonet]

The website has some of the information you are speaking about, but I don't 
think tax statements are usually provided. They have the financials there for 
your review and I have included the link as well.

http://www.nsh.org/sites/default/files/2014%20Annual%20Report%20Final.pdf


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: WILLIAM DESALVO via Histonet <histonet@lists.utsouthwestern.edu>
Sent: Thursday, January 7, 2016 4:31 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] NSH Financial and Tax Statements

As a due paying member of NSH, I ask all members and anyone interested in 
Histotechnology request from NSH office to see the Financial and Tax Statements 
for 2013 / 2014. This is public information. We should all know where our dues 
money, money from educational activities and vendor money from the convention 
is being spent.

Sent from my Windows Phone
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[Histonet] Lab Manger Position

[Walter Benton via Histonet]

Hello, thanks Walter for your assistance.

We slightly modified our posting. Here is the updated version. For more 
information about our lab, feel free to visit our 
website.<http://www.icmdiagnostics.com>

Please contact one of the two Pathologists listed below if you are interested 
in more information -
adnan.k...@icmdiagnostics.com <mailto:adnan.k...@icmdiagnostics.com> (Medical 
Director)
tom.hueb...@icmdiagnostics.com<mailto:tom.hueb...@icmdiagnostics.com> (Chief 
Pathologist)

Summary Description:
Directs and controls the day-to-day Pathology/Histology department operations 
including personnel activities, technical oversight and work flow.

Essential Functions:
* Manages all histology activities, including recruiting, hiring and training 
of staff.
* Ensures competency, motivates and encourages professional growth.
* Controls costs by monitoring productivity, personnel utilization, overtime, 
material usage rates, analyzing fluctuations in types and volumes of tests, and 
implements corrective actions.
* Ensures compliance with regulatory and accreditation agencies' rules and 
regulations.
* Designs and implements effective risk control processes.
* Researches and resolves client problem/issues.
* Oversees and coordinates startup and implementation activities resulting from 
new services or transfer of services.
* Participates in department, inter-department, inter-facility, and 
inter-regional level projects which help the regional laboratory achieve its 
goal of providing quality service and client support in a cost effective manner.
* Develops transition plan for new services/tests, outreach programs for 
transfer of work, timelines, and monitors milestones to achieve service 
expectations.
* Coordinates internal resources to support new service.
* Develops needs analysis as appropriate.

Basic Qualifications:
Experience
* Minimum five (5) years of experience in high-volume histology laboratory 
required.
* Minimum three (3) years of supervisory/managerial experience.
Education
* Bachelor's degree in biological sciences or related field required.
License, Certification, Registration
* Certification by the American Society for Clinical Pathologists.

Location:
Greenbelt, Maryland

Thank you,
Adnan


Adnan R. Khan, MD
Medical Director
Integrated Cellular & Molecular Diagnostics
P: 844.522.4263 | F: 301.363.1099 | C: 
703.282.1796
adnan.k...@icmdiagnostics.com<mailto:adnan.k...@icmdiagnostics.com>
www.icmdiagnostics.com<http://www.icmdiagnostics.com/>


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
CONFIDENTIALITY NOTICE: The information contained in this electronic message is 
intended solely for the personal and confidential use of the designated 
recipient(s) named above and may contain information that is protected from 
disclosure under applicable law. If you are not the intended recipient, or the 
employee or agent responsible for delivering it to the intended recipient, you 
are hereby notified that any dissemination, distribution or copying of this 
transmission is strictly prohibited. If you have received this transmission in 
error, please notify the transmitting person/department immediately by email or 
telephone (410) 581-5881 and delete the message without making a copy.
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Re: [Histonet] Patient identifiers

[Walter Benton via Histonet]

Full name and date of birth.

-Original Message-
From: Cartun, Richard via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, September 15, 2015 10:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Patient identifiers

What are people accepting for the "two" identifiers on the small formalin 
bottles that are used for prostate biopsy specimens?  Can you accept the 
patient's initials in place of the full name?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory Director, Biospecimen Collection Programs Assistant 
Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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Re: [Histonet] Automatic coverslippers

[Walter Benton via Histonet]

Jessica,

I have the same Leica stainer with the attached coverslipper. CV 5030. It uses 
glass coverslips in varying sizes (you choose) based on what works for your 
lab. If you have a large volume of work to coverslip each day this will 
definitely save you time. More importantly, the coverslipping will not vary, as 
it can from tech to tech. The slides can be dry in 24-48 hours completely, but 
that is determined more by the mountant type and volume being applied. Archival 
is fine with no problems since the glass system is not as particular as the 
tape from other vendors. ***Tape is faster though.***

As for common issues that you may experience if the unit is not cleaned and 
maintained properly. Broken coverglass can cause jams or even worse break 
slides. The mountant needle can bend if the brush is allowed to dry and becomes 
hard, which can then cause your slides to receive little to no mountant on 
them. The arm that picks up slides can get out alignment, but this can easily 
be fixed with a restart (re-initialization) of the coverslipper.

As for removing the left facing side of the unit, I think that can be done to 
retrofit the instrument with the link system, but check with your Leica rep to 
confirm. Linking the two systems removes the need for constant interaction by 
techs loading and unloading racks throughout the day.

The take home message to all of this is, keep it clean and it generally works 
great every day without issue. If you don't it will eventually cause you havoc.

We are very happy with our unit.

If you have any other questions, please don't hesitate to reach out to me.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

-Original Message-
From: Piche, Jessica via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, September 09, 2015 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automatic coverslippers

Hey Everyone,

I've been tasked to collect information on automatic coverslippersI was 
wondering what everyone else is using out there? Do you like them? Do they make 
your lives easier or more difficult? How do the slides hold up over time? We 
have a Leica Autostainer XL. Can it be retrofitted for an Autostainer?

Thanks in advance>:)

Jessica Piche, HT(ASCP)
Waterbury Hospital



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Re: [Histonet] Opening of Uterus' and Colons

[Walter Benton]

Here is a link to the CLIA qualifications for testing personnel. I would 
imagine that staff member you are describing is not qualified to open these 
specimens for fixation.

http://www.gpo.gov/fdsys/granule/CFR-2011-title42-vol5/CFR-2011-title42-vol5-sec493-1489


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Timm, Amber L [mailto:ti...@dxandtx.com]
Sent: Thursday, May 28, 2015 9:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Opening of Uterus' and Colons

Good morning-

I am wondering if are there regulations on who is able to open uterus' and 
colons? We have a non-Histo tech lab assistant in our laboratory and am unable 
to find any regs. on if he can open these for placing in formalin. Thoughts 
please.

Thank you

Amber Timm, MLS (ASCP)CM


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[Histonet] Temporary Position

[Walter Benton]

Temporary Histotech needed for a Urology Pathology lab in Glen Burnie, MD. This 
is a 40 hour/week position and would be for approximately 4 weeks, from 6/29/15 
- 7/24/15.  Great working environment and highly competitive compensation. If 
interested, please email your resume to mhw...@cua.md.


Thanks,

Mike

Michael Hwang
Director of Recruitment
Chesapeake Urology
25 Crossroads Drive, Suite 306
Owings Mills, MD 21117
Office: 443-738-2887
Fax:  443-738-2734
ChesapeakeUrology.com


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

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RE: [Histonet] reference lab for diagnosis of Hirschsprung's disease in an adult patient

[Walter Benton]

http://www.mayomedicallaboratories.com/test-catalog/search.php?search_type=diseasesearch=Hirschsprung+Disease

https://www.genetests.org/search/tests.php?search=Hirschsprung%20Disease,%20Sequencing%20RET%20Genesubmit=Searchstart=0types%5B%5D=from=tests

https://aravindachakravartilab.org/pro/Genetic_Testing_And_Counseling.html


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Powers, Kerry
Sent: Friday, March 20, 2015 1:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] refernce lab for diagnosis of Hirschsprung's disease in an 
adult patient

We are looking for a reference lab, in the united states, that can help us with 
the diagnosis of Hirschsprung's disease in an adult patient.  Any assistance 
would be greatly appreciated.

Kerry Powers, HTL(ASCP)
Histology Supervisor
Comanche County Memorial Hospital
3401 W. Gore Blvd
Lawton, Ok 73505
P: 580-355-8699 ext 3359
F: 580-585-5462
powe...@ccmhonline.com

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[Histonet] RE: Frosted slides

[Walter Benton]

Leica/Surgipath Slides X-tra Thermal works well. 3800090 is white and there are 
other colors as well.
Avantik has a slide as well, but I have not tried their slide.

Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen
Sent: Friday, February 27, 2015 10:36 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frosted slides

Good Friday to all! We have just setup a Slide Mate printer and the slides we 
are using the frosted area is not quite large enough. What would be ideal is 2 
1/2 cm across and 2 or 2 1/4 cm top to bottom, plain and plus in multi colors. 
any suggestions. Thanks


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[Histonet] RE: C1q FITC

[Walter Benton]

http://www.ventana.com/product/180?type=174

http://www.thermoscientific.com/content/tfs/en/product/c1q-complement-fitc-labeled-antibody.html

http://www.abcam.com/c1q-antibody-fitc-ab4223.html


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laudon, Heather 
M
Sent: Tuesday, February 03, 2015 2:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] C1q FITC




Does anyone have a vendor with C1q FITC availability? We typically order Dako 
product #F0254 which is a polyclonal rabbit anti-human C1q complement, but are 
told they are on backorder for an undetermined amount of time (possibly May 
2015). Thanks for your help!



Heather Laudon, BS, HTL (ASCP)cm

Histology Technician

Surgical Pathology Laboratory

Gundersen Health System

1900 South Avenue

Lacrosse, WI 54601

Mail Stop: H04-008

(608)775-3139


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RE: [Histonet] Histotechnology certification for cytotechnologists

[Walter Benton]

Bob,



Here are the requirements straight from the ASCP/BOC

http://ascp.org/Board-of-Certification/GetCertified#tabs-1



For Histotechnician:

Application Fee: $200

To be eligible for this examination category, an applicant must satisfy the 
requirements of at least one of the following routes:

Route 1: Successful completion of a NAACLS accredited Histotechnician program 
within the last 5 years prior to the date of application for examination; OR

Route 2: At least 60 semester hours (90 quarter hours) of academic credit from 
a regionally accredited college/university, with a combination of 12 semester 
hours (18 quarter hours) of biology and chemistry (must include credit hours in 
both), or an associate degree from a regionally accredited college/university, 
with a combination of 12 semester hours (18 quarter hours) of biology and 
chemistry(must include credit hours in both), AND one year full time acceptable 
experience in a histopathology (clinical, veterinary, industry or research) 
laboratory in the U.S., Canada or an accredited laboratory* within the last ten 
years.

*CMS CLIA certificate of registration, compliance, accreditation; AND/OR
CAP, AABB, Joint Commission accreditation; OR
JCI accreditation; OR
Accreditation under ISO 15189.

Laboratory Experience
To fulfill the experience requirement for the Histotechnician examination, you 
must have experience within the last ten years in ALL of the following areas:

 *   Fixation
 *   Microtomy
 *   Processing
 *   Staining

For Histotechnologist:



Application Fee: $225

To be eligible for this examination category, an applicant must satisfy the 
requirements of at least one of the following routes:

Route 1: Baccalaureate degree from a regionally accredited college/university 
with a combination of 30 semester hours (45 quarter hours) of biology and 
chemistry (must include credit hours in both), AND successful completion of a 
NAACLS accredited Histotechnician or Histotechnology program within the last 5 
years; OR

Route 2: Baccalaureate degree from a regionally accredited college/university 
with a combination of 30 semester hours (45 quarter hours) of biology and 
chemistry (must include credit hours in both), AND one year full time 
acceptable experience in a histopathology (clinical, veterinary, industry or 
research) laboratory in the U.S., Canada or an accredited laboratory* within 
the last ten years.

*CMS CLIA certificate of registration, compliance, accreditation; AND/OR
CAP, AABB, Joint Commission accreditation; OR
JCI accreditation; OR
Accreditation under ISO 15189.

Clinical Laboratory Experience
To fulfill the experience requirement for the Histotechnologist examination, 
you must have experience within the last ten years in ALL of the following 
areas:

 *   Fixation
 *   Microtomy
 *   Processing
 *   Staining

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond 
[rsrichm...@gmail.com]
Sent: Wednesday, September 17, 2014 9:08 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histotechnology certification for cytotechnologists

With declining utilization of gynecologic cytology (Pap smears)
opportunities for cytotechnologists are decreasing, and some of them are
considering adding histotechnology to their skills and certifications. One
small hospital's pathology service I'm acquainted with is trying to retain
its cytotechnologist by cross-training her as a histotechnologist.

Certification as a cytotechnologist requires all of the course work needed
for histotechnologist certification, so that isn't a problem. And
cytopreparation requires some of the needed skills for histotechnology -
though obviously not the primary skill of cutting paraffin. What are the
requirements for a cytotechnologist to sit the registry examination for a
histotechnologist? Is the National Society for Histotechnology (NSH)
considering addressing this issue?

Bob Richmond
Samurai Pathologist
Maryville TN
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[Histonet] IHC on free floating slices of brain

[Walter Benton]

Does anyone have information they could share on performing IHC on whole mount, 
sledge microtome free floating slices of brain? A Neuropathologist colleague is 
interested in doing this.

Thanks in advance for your help.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.comhttps://www.cua.md/owa/chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.


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[Histonet] RE: paraffin

[Walter Benton]

You may want to purchase the large tack mats for staff to walk over when going 
in and out of your embedding area. These come in blue, white, or gray:
http://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652langId=-1catalogId=29104productId=5264212distype=0highlightProductsItemsFlag=YfromSearch=1searchType=PRODhasPromo=0



Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A 
[lacie.al...@providence.org]
Sent: Wednesday, August 13, 2014 6:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] paraffin

Hi all,
I am dealing with an issue with slippery floors outside of our Histology 
department.  The concern is that it is from paraffin scraps tracked on the 
bottoms of peoples shoes.  The floor is cleaned every night and an anti-slip 
agent applied.  I have tested whether our paraffin makes the floors more 
slippery and it actually makes it harder to slipalmost tacky.  I really do 
not think it is our paraffin.  I think it is a thin layer of dust that settles 
on the floors after they are cleaned.  I have seen this happen in other places. 
 Is anyone dealing with this?  Any insight?
Thanks,
Lacie

Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.al...@providence.orgmailto:lacie.al...@providence.org


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RE: [Histonet] Are Paraffin Blocks Biohazard

[Walter Benton]

Dawn,

Take a look at this document. It should help you.
IATA and DOT have plenty of documents and training that you can take to 
understand this better.

http://www.ncsu.edu/ehs/dot/Bio_shipping.pdf

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge 
[drbu...@gmail.com]
Sent: Friday, August 08, 2014 2:40 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Are Paraffin Blocks Biohazard

Hello Histo World!

Our pathologist for our private GI lab would like me to find out if anyone
has done a study to determine if the paraffin blocks, once they have been
processed, are considered biohazard.  I have searched high and low and can
find many people stating that the blocks are not bioharzard, with the
exception of neurological tissue, but they don't state how they know this.
He would like me to reference an actual study to prove that someone has
actually looked into this.

Any one know of something like this?  I know common sense would say that
once the tissues have been in formalin for hours, than on the processor for
hours that the tissue would be non biohazard and completely safe.

Thanks for your help :)

--
Dawn R Bugge HT(ASCP), Lab Manager
Seattle Histology
Dawns Usborne Books Website http://x3128.myubam.com/
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[Histonet] RE: Plus slides for Thermo SlideMate

[Walter Benton]

Teri,

We have found that these slides work the best:

Leica
Part # 3800090
SLIDE WHITE Xtra Thermal (Color should not matter, we tried several different 
colors with good results)


Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson 
[tjohn...@gnf.org]
Sent: Wednesday, August 06, 2014 12:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Plus slides for Thermo SlideMate

Dear all,

For those of you using the SlideMate, can you give me a list of compatible 
slides for these units?

Thanks!

Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752

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RE: [Histonet] problems Sllde Mate print

[Walter Benton]

Gudrun,



We have 2 slide mates and we run our slides through all reagents and do not 
have the issue you describe. The only issue we experienced was poor print 
quality due to the type of paint on the frosted end of the slides. I recently 
switched to Leica's charged slide that is designed for Thermal Printers like 
the slide mate and the print quality is top notch and does not fade, peel, or 
smear when touched and ran through xylene, alcohol, retrieval solutions etc



Part #
3800090 SLIDE WHITE Xtra Thermal



Are your ribbons old?

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang 
[gu.l...@gmx.at]
Sent: Thursday, July 24, 2014 2:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] problems Sllde Mate print

Dear histonetters,

we have problems with the stability of the print of our slide mates
(Thermo).

We use regular slides with frosted end. The print usually looks good and
stable at the time of printing.

But after staining the print comes off like peeled skin. Very easy to
remove.



We take care, that the print doesn't come in contact with organic solvents
(deparaffination reagens = Histo SAV, butyl acetate), but without any
logical reason the first line of the print peels off and the second and
third line is rather ok.



Has anyone seen similar problems and solved them?



Gudrun Lang



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RE: [Histonet] Refrigerator Temps

[Walter Benton]

Most reagents have an acceptable range on the label that goes from 2-8 degrees 
Celsius provided they have been commercially prepared.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fran Pearsall 
[fpea...@clemson.edu]
Sent: Wednesday, July 02, 2014 8:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Refrigerator Temps

I am wanting to know what the acceptable range is for a refrigerator
which has Histo special stains and kits kept in it? We have gone from a
mercury to a digital thermometer. Any input would be appreciated. Thanks.

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RE: [Histonet] Finding a good histology supplies company

[Walter Benton]

Cytospin via Fisher 
http://www.thermoscientific.com/content/tfs/en/product/cytospin-4-cytocentrifuge.html
Thinprep by Hologic http://www.hologic.com/en/cytology/
Fisher has a similar instrument to the Thinprep (SureThin) that they are 
selling as well. 
http://www.fishersci.com/ecomm/servlet/fsproductdetail_10652_16861456__-1_0

See which one you may be interested in and then check with the vendors that 
sell refurb equipment.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of loveyadam...@yahoo.com 
[loveyadam...@yahoo.com]
Sent: Tuesday, June 17, 2014 8:00 AM
To: Histonet
Subject: [Histonet] Finding a good histology supplies company

Good morning Histonetters,

I need help finding a reasonable price cytology processor and reagents for the 
machine as well . Who's the best in our field?

Thank you in advance
Marie Adams

Sent from Yahoo Mail on Android

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[Histonet] RE: HTL Online Courses?

[Walter Benton]

Harford is an AA program which would not qualify you for an HTL, unless you 
already have a BS in Science, which in that case you have other avenues of 
eligibility for the exam.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wineman, Terra 
[terra.wine...@novusint.com]
Sent: Tuesday, June 03, 2014 1:24 PM
To: Smith, Denise; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: HTL Online Courses?

Hartford Community College in Maryland has an online course.  I would also look 
at Indiana University.

Terra Wineman, HTL (ASCP)CM
Research Biologist, Nutrional Physiology
636-926-7476 phone
terra.wine...@novusint.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith, Denise
Sent: Tuesday, June 03, 2014 12:18 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] HTL Online Courses?

Hi all!

I have been doing massive research on this for past several days but no solid 
leads.  I am wondering about HT/HTL Histology online courses... I live in St. 
Louis Missouri and they don't offer any histology courses around here - only 
out of states.  I cannot relocate or do 1-2 years program out of state due to 
my full-time job and family reason.

Do anyone know any good Histology online courses that I can take without 
conflicting with my job?

Greatly appericated!  Thanks!

Denise Smith

smit...@kids.wustl.edumailto:smit...@kids.wustl.edu




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[Histonet] RE: Xylene testing ANP 08216

[Walter Benton]

Annually

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie 
[valerie.han...@parrishmed.com]
Sent: Tuesday, April 15, 2014 11:46 AM
To: 'Piche, Jessica'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Xylene  testing ANP 08216

We test every six months.

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
Sent: Tuesday, April 15, 2014 11:33 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene testing ANP 08216

Hey Everyone,

Just curious what people are doing with xylene testing? Are people really only 
doing the initial testing and never testing again if all was good? Seems like 
there should be periodic testing after decades have gone by!

Thanks,

Jessica Piche, HT(ASCP)
Waterbury Hospital



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[Histonet] RE: ANP08216 Formaldehyde and xylene testing

[Walter Benton]

Annually for that as well

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica 
[jpi...@wtbyhosp.org]
Sent: Tuesday, April 15, 2014 12:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ANP08216 Formaldehyde and xylene testing

Thanks for all the responses so quickly. I'd also like to know the frequency of 
formaldehyde testing as well? Thanks to those who included that as well.

Jessica Piche, HT(ASCP)



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the use of the individual or entity named above. The authorized recipient of 
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[Histonet] RE: Waste Flammable Info Please

[Walter Benton]

I agree. We have to complete an EPA form for waste generated and hauled away, 
not purchased.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala 
[l...@premierlab.com]
Sent: Tuesday, April 15, 2014 2:16 PM
To: Teri Johnson; 'dennis.h...@cookchildrens.org'
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Waste Flammable Info Please

Teri

That's interesting since we need to fill out a form every year that tabulates 
the waste generated and not what we purchase.  I thought it was based upon the 
amount of waste generated.

That was not our experience.  The state came and audited us once we started 
disposing of waste at a rate of 55 gallons per month.  That pushed us past that 
650 gallons for a conditionally exempt small quantity generator and therefore 
we became a small quantity generator.  They never asked us how much we 
purchased only what the waste stream was.

Just my experience.  Since the states monitor for this, it may vary from State 
to State how the EPA regs are interpreted or if State laws override federal 
regarding waste and classifications.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson
Sent: Tuesday, April 15, 2014 12:00 PM
To: 'dennis.h...@cookchildrens.org'
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Waste Flammable Info Please

Hi Dennis,

I don't think that recycling will fix your EPA license issue. They care about 
how much you GENERATE through use, not whether it is actually being disposed of 
or even recycled. We had an issue with the EPA regarding our xylene generation 
at another facility I used to work in. When I tried to point out that we were 
recycling it, they said that didn't make a difference. Once it goes into use, 
it becomes generated waste.

You might want to look at changing your practices so that you generate less, 
i.e. changing the processors according to block numbers or alcohol dilution 
rather than number of runs, or using smaller containers at the staining bench.

Alcohol recycling is great, but you will never get back anything more pure than 
about 98% so you will need to continue to purchase absolute alcohol. The 
biggest difficulty is making sure you keep any xylene contaminated alcohol 
separate and out of the recycler. Xylene will not be removed from alcohol 
during alcohol recycling so you will still have contaminated alcohol. Anything 
containing xylene must be recycled as xylene (or manifested out as waste).

I hope this helps,

Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752

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[Histonet] RE: Gross only

[Walter Benton]

It should be 88300

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya 
[tanyaabb...@catholichealth.net]
Sent: Wednesday, February 26, 2014 11:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gross only

How does everyone bill for Gross Only?
Thanks!

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Searching for Available Histo Position in New Mexico

[Walter Benton]

Does anyone know of any positions in the Albuquerque, New Mexico? I have a 
colleague looking for a position there.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.comhttp://www.chesapeakeurology.com/

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



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[Histonet] RE: Cytolyt Solution

[Walter Benton]

Hologic

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rolly Perez 
[rpe...@medsurgpath.com]
Sent: Wednesday, February 19, 2014 2:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cytolyt Solution

Anyone knows how to make Cytolyt Solution?



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[Histonet] RE: Glassware Cleaning

[Walter Benton]

Fisher sells a RTU solution of the bromcresol purple. We use that for our 
glassware. It works very well.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew D. Roark 
[mro...@sfmc.net]
Sent: Thursday, February 13, 2014 1:02 PM
To: Histonet@Lists. Edu
Subject: [Histonet] Glassware Cleaning

What is everyone using to test their glassware for detergent residue? Is there 
a ready to use solution or not?


CAP Checklist...

Glassware Cleaning Phase II

There are appropriate documented procedures for handling and cleaning 
glassware, including
methods for testing for detergent removal.

NOTE: Special instructions for micropipettes, cuvets, acid washing, etc. must 
be included.

A simple procedure to check for detergent residue uses bromcresol purple (0.1 g 
bromcresol purple
in 50 mL ethyl alcohol). Pipette approximately 5 cm (2 inches) distilled water 
into a representative,
washed, glassware item. Add two to three drops bromcresol solution. A purple 
color reveals residual
detergent. A yellow color indicates satisfactory rinsing.

Thanks!


Matthew Roark- HT/HTL(ASCP)CM
Histology Specialist
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-3982
mro...@sfmc.net
http://www.sfmc.net




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RE: [Histonet] Lysol I.C.

[Walter Benton]

Lab Safety Supply sells it as well.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita 
[azdud...@hotmail.com]
Sent: Thursday, January 23, 2014 11:09 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Lysol I.C.

We are using Lysol I.C. for disinfecting the ventana benchmark.  I thought I 
purchased it from Cardinal but I'm not sure now where we got it.  Are others 
using this for their machine and where are you purchasing it from?   Thanks so 
much.



Anita Dudley

Providence Hosp.

Mobile, Al. 36695
  
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[Histonet] RE: p16

[Walter Benton]

I believe Ventana owns the rights to that antibody now. It was previously owned 
by MTM Labs.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt 
[nancy_schm...@pa-ucl.com]
Sent: Thursday, January 23, 2014 11:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16

Good Morning!

Where are you currently obtaining p16 from?

Thanks a bunch!

Nancy

Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories
Please visit our website: http://www.uclaccess.com/




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[Histonet] RE: Retaining unstained glass slides

[Walter Benton]

Follow the guidelines of your accrediting agency.

CAP = 10 years minimum

ANP.12500 Record Retention Phase II
Surgical pathology records and materials are retained for an appropriate period.
NOTE 1: Minimum requirements for surgical pathology, providing these are not 
less stringent than
local, state and national regulations, are:
1. Accession log records - 2 years
2. Wet tissue (stock bottle) - 2 weeks after final report
3. Paraffin blocks - 10 years (subject to Note 2, below)
4. Glass slides (including control slides) and reports - 10 years (slides must 
remain
readable for this period)
5. Surgical pathology reports - 10 years (see Notes 3 and 4, below)
6. Fluorochrome-stained slides - at the discretion of the laboratory director
7. Fine needle aspiration slides - 10 years
8. Images of FISH studies - 10 years (see Note 5, below)

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Renee H. Workman 
[rhwork...@uro.com]
Sent: Wednesday, January 22, 2014 1:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retaining unstained glass slides

How long due most labs retain glass slides?
Renee H. Workman
Histology Supervisor
Virginia Urology
9105 Stony Point Drive
Richmond, VA  23235
W: 804-527-1316 | F: 804-270-0917
rhwork...@uro.commailto:rhwork...@uro.com | www.uro.comhttp://www.uro.com





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RE: [Histonet] microwave processing

[Walter Benton]

Perhaps you can get some literature from one of the vendors that sell that 
technology.

Milestone Medical
Sakura


Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang 
[gu.l...@gmx.at]
Sent: Monday, January 13, 2014 1:56 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] microwave processing

Hi!

Can someone recommend literature about microwave processing. I'm interested
in the physical principles behind the process. And I want to get answers to
the questions: why is this microwave-assisted infiltration faster? What
happens to proteins /antigens under microwave radiation? Is there a
difference between conventional or microwave processing in relation to
antigen preservation after usual formalinfixation.



Thanks in advance

Gudrun Lang





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[Histonet] RE: cryostat

[Walter Benton]

ANP.23410 Cryostat Decontamination Phase II
There is a documented procedure for the routine decontamination of the cryostat 
at defined
intervals, and decontamination records are evident.
NOTE: The cryostat must be defrosted and decontaminated by wiping all exposed 
surfaces with
tuberculocidal disinfectant. The cryostat should be at room temperature during 
decontamination
unless otherwise specified by the manufacturer. This should be done at an 
interval appropriate for
the institution; this must be weekly for instruments used daily. Trimmings and 
sections of tissue
that accumulate inside the cryostat must be removed during decontamination. 
Although not a
requirement, steel mesh gloves should be worn when changing knife blades.
REFERENCES
1) Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory 
Workers From Occupationally Acquired Infections; Approved
Guideline—Third Edition. CLSI document M29-A3 (ISBN 1-56238-567-4). Clinical 
and Laboratory Standards Institute, 940 West Valley
Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA, 2005.
2) http://www.well.ox.ac.uk/_asset/file/leica-disinifection-2.pdf
3) http://www.epa.gov/oppad001/list_b_tuberculocide.pdf

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L 
[dorothy.l.w...@healthpartners.com]
Sent: Thursday, January 02, 2014 11:44 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] cryostat

Does anyone have any current information on the cryostats with UV for 
decontamination?  Specifically, is it accepted as a means of decontamination by 
CAP or JS or any other regulatory agencies?  Basically, is it worth the extra 
dollars to go that route?

Much thanks and Happy 2014!!

Dorothy Webb, HT (ASCP)
Regions Histology Technical Specialist
651-254-2962



  
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RE: [Histonet] Hot Topic Medicare Code

[Walter Benton]

AMA prescribes codes 88342 and 88343 for qualitative IHC (Non-Medicare):
The AMA's CPT-2014 codebook offers two codes for reporting with qualitative 
immunohistochemistry (IHC) testing. The codes and official parenthetical 
instructions for their use starting Jan. 1 are as follows:
88342  Immunohistochemistry or immunocytochemistry, each separately 
identifiable antibody per block, cytologic preparation, or hematologic smear; 
first separately identifiable antibody per slide
(Do not report 88342 in conjunction with 88360 or 88361 for the 
same antibody)
(For quantitative or semi-quantitative immunohistochemistry, see 
88360, 88361)
88343 each additional separately identifiable antibody per slide (List 
separately in addition to code for primary procedure)
  (Use 88343 in conjunction with 88342)
  (When multiple antibodies are applied to the same slide, use one unit of 
88342 for the first separately identifiable antibody and one unit of 88343 for 
each additional identifiable antibody)

CMS prescribes codes G0461 and G0462 for qualitative IHC:
CMS will not accept CPT code 88342 or 88343 on a claim effective with dates of 
service on and after Jan. 1, 2014: Those two codes are not valid for Medicare 
purposes and will be summarily denied if billed. To report a professional, 
technical or global charge for qualitative immunohistochemistry (IHC) testing 
for a Medicare beneficiary on and after Jan. 1, 2014, you must use the 
applicable HCPCS Level II code as follows:
G0461 Immunohistochemistry or immunocytochemistry, per specimen; first single 
or multiplex antibody stain
G0462   each additional single or multiplex antibody stain (List separately 
in addition to code for primary procedure)
Codes G0461 and G0462 have 26 and TC modifier lines in the 2014 physician fee 
schedule, so you'll bill them using the modifier (or no modifier, if entitled 
to bill the global service) that applies to your practice and any given 
Medicare beneficiary claim.
CMS prescribes that you continue to bill for qualitative IHC testing 'per 
specimen' as you have since Jan. 1, 2012. Furthermore, you'll continue to bill 
for quantitative IHC testing 'per specimen' using CPT codes 88360 and 88361 
just as you do today and have done since Jan. 1, 2012.


Effective January 1, 2014, per CMS (Medicare) all prostate needle biopsies, any 
method will be reported with the appropriate HCPCS code. They are:



G0416-Surgical pathology, gross and microscopic examination for prostate needle 
biopsies, any method: 10 to 20 specimens  (This is the code that pertains to 
most of our Prostate Needle Biopsy Cases)



G0417- Surgical pathology, gross and microscopic examination for prostate 
needle biopsies, any method: 21 to 40 specimens



G0418- Surgical pathology, gross and microscopic examination for prostate 
needle biopsies, any method: 41 to 60 specimens



G0419- Surgical pathology, gross and microscopic examination for prostate 
needle biopsies, any method: greater than 60 specimens



Per CMS, the descriptor was changed to say any method and is no longer tied 
to the surgical approach. So now it will be the count of individual biopsies 
that will tell you how to code your case. If you have 12 vials, you will report 
G0416. Please note that for nine or less you will continue to report 88305 per 
the number of separately identified biopsies. This will apply for CMS patients 
nationwide.



Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mary Ann Deathridge 
[madeathri...@pastnashville.com]
Sent: Thursday, January 02, 2014 5:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hot Topic Medicare Code

HAPPY NEW YEAR HISTONETTERS!

Ok, so here is a question for 2014 coders.How do you code ( or should I
say get to code) a doublestain for Medicare and for non-medicare?

G0461 and G0462 (Medicare) and 88342 and 88343 for non-medicare. Can you
charge for both antibodies in the doublestain or do you only charge one
code and eat the second antibody on the same slide.



Thanks for replies in advance.

Maryann Deathridge, BS, HT (ASCP)

Lab Manager

Pathology Assoc.. of St. Thomas

615-298-4100, Fax: 615-298-4141



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[Histonet] RE: Freezer for tissue storage

[Walter Benton]

Revco freezers are very good. I prefer uprights for their ease of use (with 
racks and seeing the stored samples) and use of doors over stryofoam covers 
used for chest freezers.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of King, Laurie J 
[king.lau...@marshfieldclinic.org]
Sent: Thursday, December 12, 2013 1:57 PM
To: Cartun, Richard; Histonet
Subject: [Histonet] RE: Freezer for tissue storage

In my experience the uprights have more frost buildup problems, causing sealing 
problems. The chest ones I've had in the lab do less of this.

Laurie

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Thursday, December 12, 2013 12:52 PM
To: Histonet
Subject: [Histonet] Freezer for tissue storage

I need a recommendation for a -80 degree C. freezer for storing tissue 
specimens.  Do you prefer upright vs. chest?  Thanks.



Richard



Richard W. Cartun, MS, PhD

Director, Histology  Immunopathology

Director, Biospecimen Collection Programs

Assistant Director, Anatomic Pathology

Hartford Hospital

80 Seymour Street

Hartford, CT  06102

(860) 545-1596 Office

(860) 545-2204 Fax

richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org

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RE: [Histonet] Shandon Grosslab Senior

[Walter Benton]

Try Mercedes Medical

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Stephenson 
[cls71...@gmail.com]
Sent: Thursday, December 05, 2013 12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Shandon Grosslab Senior

Hello,

Does anyone that uses this grossing station have a great (cheaper than
ThermoScientific) place to purchase filters?

Thanks,
Cristi
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RE: [Histonet] cryostat service

[Walter Benton]

Bel Air Instruments and Dolbey Jamison

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni 
[trathbo...@somerset-healthcare.com]
Sent: Monday, October 28, 2013 2:29 PM
To: 'Jean Ross'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat service

Avantik is located in NJ and services cryostats in addition to microtomes, 
processors and stainers.
www.avantik-us.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean Ross
Sent: Monday, October 28, 2013 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat service

Hi everyone,

I am interested in finding out if there is anyone in or close to Delaware that 
services and repairs cryostats.  Any recommendations would be much appreciated.

Thanks,
Jean
--
Jean Ross
Delaware Biotechnology Institute, BioImaging Center University of Delaware
15 Innovation Way
Suite 117
Newark, DE  19711
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[Histonet] RE: Can someone recommend a good labeling pen for cryotubes.

[Walter Benton]

Are you using the red label Industrial Sharpie already, if not give them a 
try?

I would also suggest using a slide marking pen since they are resistant to many 
solvents used in the lab like alcohol and xylene.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

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From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick 
[patrick.le...@seattlechildrens.org]
Sent: Wednesday, October 23, 2013 1:54 PM
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Can someone recommend a good labeling pen for cryotubes.

Hi everyone,

Can someone recommend a good pen to use to label cryo tubes.The VWR pens we 
have been using are apparently discontinued.

I would like something that writes as fine as an ultrafine sharpie, but is less 
likely to smear and is more long lasting, and solvent tolerant.

Thanks

Patrick.


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[Histonet] RE: thawing and refreezing muscle?

[Walter Benton]

This may help. Never used it when I processed fresh/frozen muscle specimens but 
have heard good things.

http://www.cell-ess.com/Nonfrozen_Transport_Medium_Preserves_and_Restores_Skeletal_Muscle_Enzymatic_Activity_and_Morphology.pdf

http://www.lifebloodmedical.com/

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy 
[timothy.mor...@ucsfmedctr.org]
Sent: Tuesday, October 01, 2013 12:07 PM
To: Histonet
Cc: jmitch...@neurology.wisc.edu
Subject: [Histonet] thawing and refreezing muscle?

Histonetters, Does anyone have a good method for thawing muscle and refreezing 
for histochemistry? With good results? We have some bulk-frozen muscle 
(centimeter thick) and that is what they want to try.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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RE: [Histonet] Flammable Liquid

[Walter Benton]

https://www.osha.gov/dte/library/flammable_liquids/flammable_liquids.html

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni 
[trathbo...@somerset-healthcare.com]
Sent: Tuesday, September 17, 2013 12:14 PM
To: 'Rene J Buesa'; Anne Murvosh; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Flammable Liquid

Is this on the CLIA website? I agree that it's difficult to find anything on 
there. If you're able to provide a direct link that would be great!


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, September 17, 2013 11:56 AM
To: Anne Murvosh; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Flammable Liquid

The standard is 1 gallon per every 24 square feet of lab area, so if your lab 
has 240 square feet surface, you can store 10 gals of flammable liquids.
René J.



From: Anne Murvosh amurv...@advancederm.net
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, September 17, 2013 11:23 AM
Subject: [Histonet] Flammable Liquid


Can anyone tell me how many gallons of Flammable liquid can be stored in
a space per CLIA regulations.  There website is useless.  Thanks Anne

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[Histonet] RE: Microtome Service

[Walter Benton]

http://www.dlrepair.com/index.php
http://dolbeyjamison.com/
http://belairinc.com/

Try any of these companies. I have used them all and they do quality work.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar 
[fawn.bo...@halifaxregional.com]
Sent: Tuesday, September 10, 2013 6:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome Service

Does anyone know of any company that is able to service the Leica RM2155 
microtomes?  We are located in South Boston, Va.



Thank You



Fawn Bomar
-
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RE: [Histonet] (no subject)

[Walter Benton]

Stacey,

In addition to the 70% step, many processors (not sure about this one had ways 
to perform a warm water flush) to rinse the lines of salt precipitate.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of STACEY 
[stace...@sbcglobal.net]
Sent: Monday, July 15, 2013 1:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Our laboratory has a Leica Peloris processor. Currently, we are using 
unbuffered 10% formalin. We are in the process of switching to 10% neutral 
buffered formalin. There is some concern about salt build-up within the 
processor, as the cleaning cycle only consists of cleaning xylene and cleaning 
alcohol. I spoke to a representative at Leica who maintains that if you use 1 
or 2 stations containing 70% alcohol immediately after the formain stations, 
that this is enough to prevent salt build-up from the buffered formalin. One of 
my technicians has some concern about this procedure. I would appreciate any 
feedback on this issue, especially from another lab using the Leica Peloris 
with 10% neutral buffered formalin. Thank you.

Stacey Merica H.T.
Histology Supervisor
North Kansas City Hospital
North Kansas City, MO
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RE: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides

[Walter Benton]

http://www.ihcworld.com/products/IHC-Staining-System.htm

I have not used, but it seems that it would work like the old ones.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
davidclar...@comcast.net [davidclar...@comcast.net]
Sent: Monday, July 08, 2013 2:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides

Hi Histonetters,


I hope you all had a wonderful Independence Day!


Do you guys remember in the olden days before automation when we did IHC by 
hand?


I'm looking for an incubation chamber that allows us to incubate our slides 
overnight. The one I have right now is about 20x6x6 with a lid


and allows for about 4 flat racks of slides to be placed inside. Any 
information would be appreciated either if you have one or know of a company 
that can make one.


Cheers!


David Clark
Oregon Health  Science University
Portland, Oregon
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[Histonet] RE: phobic slides

[Walter Benton]

Bruce,

Are you using the slides with the patient and control all in one. If so, is the 
paint on the top of the slide or the reverse underside? There has been a known 
problem for years that correlates to what you are describing with the control 
box slides with paint on the top.

Secondly, have you noticed this phenomenon on one instrument or all? If it is 
one instrument I would check to make sure the vortex mixers are working 
properly. This is done easily with a drop of Hematoxylin on the slide and 
turning the vortex mixers on within the instrument.

Another possibility could be that your instrument may not be lining up with the 
holes where the slide positions are located. Not knowing which instrument you 
are using it is hard to say. The benchmark had both slide carousel and 
dispenser reagent rack moving which often caused misalignment and solutions 
would not reach the slide, bit instead would be on the drip tray. The XT can 
have the same problem, but not nearly as often since only the reagents move and 
not the slides.

As I'm sure you aware slide variability does exist within the pack of slides at 
times, (I have witnessed this personally with Asian slides specifically, never 
German or American). It truly depends on the technique being used to charge the 
slide.

Finally, you may not have seen this with Dako instrumentation since it uses a 
different mechanism to place reagent on the slide. It uses the drop zones and 
surfactant to have the solution cover the slide. I would imagine that it is 
less likely to occur on the Dako unless your slides are truly hydrophobic.

Hope this helps!

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski 
[bgapin...@pathgroup.com]
Sent: Friday, July 05, 2013 12:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] phobic slides

Histonians!
Has anyone else had incorrect  IHC  staining due to overly 
charged slides that are hydrophobic? My Ventana service department was out 
because I kept complaining about incomplete staining. For instance, the control 
at the top of the slide stained with  both DAB and hematoxylin. But on the very 
same slide the hematoxylin was missing from the patient tissue.
I told them how this unnerved me. If the same thing happens but 
the hematoxylin stains and the DAB does not...I could be looking at a false 
negative. This may have happened already as far as I know.
The solution according to Ventana is to take 4 slides from each 
new box of slides (1/2 gross) and do a vortex mix on each of them one at a 
time. Then you know you have valid slides to stain patient tissues! I have no 
time for this. And I'm trying to believe it but it's just not scientific. How 
is it that my results have been free of this phobia for over a year? Why 
didn't I get this (EVER) with Dako instrumentation? The answer, I was told, was 
that the humidity was a problem. If I were to take this to it's ridiculous 
conclusion, I would need to inform oncologists not to operate during these 
incorrectly humidified days. It IS summer so I'm not sure how much humidity is 
required.

1.   What brand of slides do you use?

2.   Have you had the slide phobia so that it compromised the results? 
(Slide-phobia is for retired Histologists, no?)

3.   (I think I know the answer but I just have to ask) Do you validate 
slides?

Bruce Gapinsk HT (ASCP)
Chief Histologist
Marin Medical Laboratories
PathGroup SF




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[Histonet] FYI Regarding HT and HTL

[Walter Benton]

http://naaclsnews.wordpress.com/2013/05/03/proposed-change-to-htl-standards/

Proposed Change to 2012 HTL Standards: Request for Public Comment
In September 2012, the NAACLS Board of Directors approved new Standards for 
Accredited and Approved Programs.  It is projected that programs will begin to 
be reviewed under these Standards starting with those programs that will be 
submitting their self-studies on or after September 1st, 2014.
The 2012 Standards for Accredited Histotechnologist (HTL) programs necessitate 
that the program director (or education coordinator, when required) hold 
“ASCP-BOC certification as a Histotechnologist”.  This is a change from the 
previous HTL Standard for program directors (approved in 2004), which stated 
that the program director (or education coordinator, when required) must be 
“certified in Histotechnology”.   NAACLS’ interpretation and practice of the 
old standard allowed for those with HTL(ASCP) or HT(ASCP) certification to be 
the program director or education coordinator of an HTL program.
The NAACLS Board of Directors is requesting public comment for a proposed 2012 
Standards change to 2012 HTL Standard 7A1B and 2012 HTL Standard 7B1B .  This 
change will allow both HTL(ASCP) and HT(ASCP) certifications to be acceptable 
for program directors and education coordinators of NAACLS Accredited HTL 
programs, as recommended by the Review Committee for Accredited Programs.  This 
period of public comment has been made available for thirty (30) days.

The current 2012 HTL Standard 7A1B reads as follows:
“…holds ASCP-BOC U.S. certification as a Histotechnologist. Program Directors 
who have been approved as a program director of a NAACLS accredited HTL program 
prior to October 1, 2013 remain eligible as a program director. If the program 
director does not hold ASCP-BOC U.S. certification as a Histotechnologist, a 
qualified professional who does hold ASCP-BOC U.S. certification as a 
Histotechnologist must hold appointment as education coordinator.”
The proposed change to 2012 HTL Standard 7A1B reads as follows:
“…holds ASCP-BOC U.S. certification as a Histotechnologist or Histotechnician. 
Program Directors who have been approved as a program director of a NAACLS 
accredited HTL program prior to October 1, 2013 remain eligible as a program 
director. If the program director does not hold ASCP-BOC U.S. certification as 
a Histotechnologist or Histotechnician, a qualified professional who does hold 
ASCP-BOC U.S. certification as a Histotechnologist or Histotechnician must hold 
appointment as education coordinator.”
The current 2012 HTL Standard 7B1B reads as follows:
“…holds ASCP-BOC U.S. certification as a Histotechnologist.”
The proposed change to 2012 HTL Standard 7B1B reads as follows:
“…holds ASCP-BOC U.S. certification as a Histotechnologist or Histotechnician.”

This request for public comment has been placed on the NAACLS website, and 
comments may be sent via letter to the NAACLS office, or by email to 
erotchf...@naacls.orgmailto:erotchf...@naacls.org.  Due to the informal 
nature of LISTSERV communications, comments or discussion posted on the CLS 
Educators LISTSERV will not be presented to or considered by the NAACLS Board 
of Directors.  All comments must be received by June 3, 2013.  The comments 
will then be reviewed and brought before the Board of Directors at the 
September 2013 Board Meeting.
Edward Rotchford, Accreditation Specialist
Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.mdmailto:wben...@cua.md



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RE: [Histonet] CEU's

[Walter Benton]

AT, BB, C, CG, CT, H,   HT, HTL, M, MB
Certified in 2004 or later require 36 points with
• 1 point in laboratory or patient safety (i.e., quality control, quality 
assurance)
• 2 points in area in which you are certified
• Remaining points in area of specialty, management,
education, or other related laboratory areas of interest

http://ascp.org/PDF/BOC-PDFs/CMP/CMPBooklet.aspx

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison 
[akemiat3...@yahoo.com]
Sent: Thursday, April 04, 2013 12:32 PM
To: Histonet
Subject: [Histonet] CEU's

Hi All:

I have been asked a question that I don't have the answer too. I need to know 
how many CEU's a registered HT/HTL is required to have to maintain their 
registry.  I am a dinosaur who received her HT in 1968 and my HTL in 1982, so 
do not need to renew mine.

Akemi Allison-Tacha, BS, HT (ASCP) HTL
Pathology Manager
Monterey Bay GI Consultants
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
(381) 375-3577  X117
Email: aalli...@montereygi.com
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[Histonet] RE: Temperature ranges

[Walter Benton]

Gail,

Water bath - Needs to be based on the type of paraffin that use since you don't 
want it to be hotter than the melting point etc It is a number range that 
you establish based on the paraffin. My experience has been between 40 to 50 
Celsius

Lab oven for slide drying - Varying opinions exist on this due to IHC stains 
etc..., but I have seen 60-80 Celsius.

Refrigerators for IHC antibodies - Depends on the contents of the refrigerator 
but most antibodies are stored at 2-8 Celsius. Concentrates or aliquots are 
sometimes frozen etc...but you need to read you package inserts etc to 
determine storage requirements

Embedding center (both the hot and cold plates and the reservoirs for the molds 
and tissue cassettes) Dependent on the paraffin and personal use. Cold plate on 
our unit is not controllable, however you can control the temp of  hot plates 
and the paraffin reservoir based on the paraffin temps recommended for your 
paraffin

Paraffin dispenser - Based on your melting point of paraffin. The bag will tell 
you a range that it should not go under or above.




Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gail Marcella 
[gmarce...@nj-urology.com]
Sent: Wednesday, January 30, 2013 9:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperature ranges

Hi - I wanted to find out if anyone knows what the normal temperature ranges 
for the following pieces of equipment are:

Water bath
Lab oven for slide drying
Refrigeratros for IHC antibodies
Embedding center (both the hot and cold plates and the reservoirs for the molds 
and tissue cassettes)
Paraffin dispenser

Thanks - Gail Marcella - NJ Urology

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RE: [Histonet] Block retention?

[Walter Benton]

Who inspects you? That is what you should follow unless your management wants 
to be more cautious and follow the CAP guidelines which you have listed. Entire 
procedure listed below.

ANP.12500 Record Retention Phase II
Surgical pathology records and materials are retained for an appropriate period.
NOTE 1: Minimum requirements for surgical pathology, providing these are not 
less stringent than
state and federal regulations, are:
1. Accession log records - 2 years
2. Wet tissue (stock bottle) - 2 weeks after final report
3. Paraffin blocks - 10 years (subject to Note 2, below)
4. Glass slides (including control slides) and reports - 10 years (slides must 
remain
readable for this period)
5. Surgical pathology reports - 10 years (see Notes 3 and 4, below)
6. Fluorochrome-stained slides - at the discretion of the laboratory director
7. Fine needle aspiration slides - 10 years
8. Images of FISH studies - 10 years (see Note 5, below)
There must be a documented policy for protecting and preserving the integrity 
and retrieval of
surgical pathology materials and records.The retention period should be 
extended, when appropriate,
to provide documentation for adequate quality control and medical care.
NOTE 2: Regarding release of blocks for research purposes: Federal regulations 
require that a
laboratory retain paraffin blocks for two years unless the tissue is blocked 
specifically for research
and not used for patient diagnostic purposes.* The CLA requires, however, that 
paraffin blocks
used for patient diagnostic purposes must be kept for at least 10 years. 
Nevertheless, such blocks
may be released for research purposes after the two-year regulatory requirement 
if all of the following
criteria are met:
1. For laboratories subject to US regulations, formal written authorization is 
obtained in
accordance with the requirements of HIPAA if identifiable patient information 
is released.
2. The laboratory retains sufficient blocks to support the diagnosis for the 
full 10-year
period.
3. Provision is made for retrieval by the laboratory of any blocks or material 
that remain
after use in research, if the blocks or material are needed for diagnostic, 
legal, or other
legitimate purposes.
4. The laboratory meets other relevant requirements including but not limited 
to the
requirements of the institution, the directives of any applicable institutional 
review board
(IRB) or similar entity; and state and local laws and regulations.
NOTE 3: Pathology reports may be retained in either paper or electronic format. 
If retained in
electronic format alone, however, the electronic reports must include a secure 
pathologist electronic
signature. Images of paper reports--such as microfiche or PDF files--are 
acceptable.
NOTE 4: Reports of outside consultations performed on cases from the laboratory 
(whether or not
such consultation was requested by the laboratory) must be retained for 10 
years after the date on
which the original report was issued.
NOTE 5: There is no retention requirement for images when the source slides 
remain readable for
the required 10-year retention period. The 10-year retention requirement 
applies to images of slide
preparations that are not readable for the 10-year period (e.g. FISH studies).

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jmasla...@stpetes.org 
[jmasla...@stpetes.org]
Sent: Thursday, January 10, 2013 12:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Block retention?

I searched for retention policies and found what seems to be an 8 year
difference in keeping blocks. How long do you retain your blocks?


CDC website CLIA
Sec. 493.1105  Standard: Retention requirements







(7) Slide, block, and tissue retention--
(i) Slides.

(ii) Blocks. Retain pathology specimen blocks for at least 2 years
from the date of examination.











CAP website states using CLIA as guide






Surgical Pathology (including bone marrows)
Wet tissue
2 weeks after final report
Paraffin blocks
10 years
Slides
10 years
Reports
10 years






















Joe Maslanka BS, CT,HT (ASCP)
Anatomical Pathology Technical Supervisor
St Peter's Hospital,MT 59601
(P)(406) 447-2406
(F)(406)444-2126

Give thanks for ALL things.
Kindness is the language the blind can see  the deaf can hear- Mark
Twain
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RE: [Histonet] Reagent Alc or Denatured Alc ?

[Walter Benton]

They are one in the same basically, meaning not fit for human consumption, by 
means of adding methanol or other impurity. Pure ethanol would require an 
alcohol license and inventory tracking since it is fit for human consumption.


Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles 
[estellamire...@gmail.com]
Sent: Wednesday, January 09, 2013 2:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Reagent Alc or Denatured Alc ?

What is the difference between the two ?

Thinking about switching to denatured becasue of cost costing.

Which do you prefer?

Stella
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RE: [Histonet] Trichrome

[Walter Benton]

Sounds like you may have a cracked vortex mixer and/or silver in your reagent 
dispensing line. If the later is the case new tubing will fix this issue and 
new vortex mixer(s) will fix the issue with mixing. Do you know how to perform 
a vortex mix test using the service menu functions? If so, place a drop of 
hematoxylin on a slide and with the lid open you will be able to see if the 
vortex mixers are blowing the solution counter-clockwise and clockwise. Each 
mixer does it in a different manner to make sure the reagents are mixed well 
over the entire slide.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar 
[tmcne...@lmhealth.org]
Sent: Tuesday, December 04, 2012 12:53 PM
To: 'Cheryl'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Trichrome

Same problem here.  Using the Nexus stainer and have also experienced this with 
the Reticulin as well.  Called service today and they elected to send an 
application specialist instead of a service tech.  Will see what happens.  
Would not have expected to see this when doing the stain by hand

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Tuesday, December 04, 2012 8:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Trichrome

Hi- need help troubleshooting a trichrome.  The stain on the Nexus stainers is 
inconsistent.  Taking it off the stainer and doing it by hand with a kit has 
similar results.

The tissue is NBF fixed needle biopsies on kidney.  There is a little 
mechanical damage on the edges of the tissue but the collagens are staining too 
light and the red overstains.  The normal tissue as control is beautiful -- I 
know I know--don't force the stain--but it doesn't make sense to have this much 
variation from a kit or auto stainer...

Help?


Cheryl Kerry, HT(ASCP)
Path Group of Louisiana

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[Histonet] RE: Slide printing systems

[Walter Benton]

I too have the same systems that are being referenced. The Printmate did have a 
ton of problems with the original print heads, however they are doing a sweep 
to replace all of them according to my rep. We no longer experience the broken 
pins or other associated issues with the Print Mate.

As for the slide mate, we had similar issues with that as well and found that 
after it was serviced a second time that we needed upgraded software/firmware 
on the instrument itself. Another issue that goes untouched by many users is 
the advanced user menu, which controls the pressure in which the thermal head 
presses on the slides and a variety of other attributes. Most reps are not 
aware of these features and thus are unable to help resolve your issues. Once 
we found out about these settings our slidemates work great.

In addition to the advance settings on the slide mate it is important to wipe 
the thermal print head with an alcohol wipe daily to keep it clean performing 
optimally. Another issue that I have witnessed is that some of the paint on 
some slides do not work as well as others. I recently tested a slide with paint 
specifically formulated for the slide mate and the print was the best I have 
ever seen.

Overall the units work well provided they are maintained and you have a 
knowledgeable support person to help you test out some of the advance menu 
features.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala 
[l...@premierlab.com]
Sent: Wednesday, August 15, 2012 11:28 AM
To: 'Bell, Pat'; 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Slide printing systems

We had the slide mate and we do not use it any more, its worthless.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Pat
Sent: Wednesday, August 15, 2012 9:26 AM
To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Slide printing systems

I also have the slide-mate and have a lot of problems with the printing. We 
also have to spend a lot of time rewriting and labeling them. I have been told 
to send it in to get it repaired but I wonder if it is worth the time and money 
if you are getting it back in basically the same condition! I know the Leica is 
pretty good but I am in research and can't spend that kind of money. I wish 
there was a system that was good and not extremely expensive.

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
RC1-S, L18-8402C
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mitchell, 
Janice A
Sent: Wednesday, August 15, 2012 8:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide printing systems

Good Morning,

We currently are using Thermo-Fisher print-mate and slide-mate.  I am not very 
happy with the printing of the slides.  Some days I feel like spend most of my 
time re-writing and labeling printed slides. Then when the units  are  sent out 
for repair and it arrives back it is not a whole lot better.  Before we get our 
new LIS system and our Docs will be scanning the bar code(that sometimes do not 
print) I need to find a better system.  I will not be able to deal with how 
often my name will be called out when the scanning of the slides fail.

I would like any input on other systems pros and cons that are currently being 
used in histology labs.   I know no system will be perfect but something has to 
be better.

Thanks, Janice

Janice A. Mitchell, BS, HT(ASCP)
Histology Lead Tech
Children's Hospital of Philadelphia
Anatomic Pathology and Laboratory Medicine
324 S. 34th Street
Philadelphia, Pa 19104-4399
215-590-1738(lab)
267-426-7754(office)


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CONFIDENTIALITY

[Histonet] RE: HT exam

[Walter Benton]

If you are referring to the removal of the OTJ portion of taking the registry 
with a H.S. diploma I think that happened sometime in the middle of 2000, maybe 
around 2004-2008. I don't recall the exact year. Check the ASCP website, it 
should be listed there or they should be able to answer that question.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth 
[kenneth.metz...@aruplab.com]
Sent: Thursday, August 09, 2012 10:49 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] HT exam

Can someone tell me what year they began requiring college credits for the HT 
exam? Thanks

Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.metz...@aruplab.com


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Unauthorized forwarding, printing, copying, distributing, or use of
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[Histonet] RE: counterstain for Alcian Blue (ph2.5)

[Walter Benton]

If I recall correctly Newcomer Supply and Poly Scientific both sell NFR.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig 
[dmcc...@ckha.on.ca]
Sent: Wednesday, August 01, 2012 12:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] counterstain for Alcian Blue (ph2.5)

Can you please let me know what options for counterstain there are other
than Nuclear Fast Red or a supplier who sells the prepared solution



Diana

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[Histonet] RE: MOHS Tech

[Walter Benton]

I would review the CLIA guidelines, which dictate minimum qualifications to 
perform certain tasks.

http://wwwn.cdc.gov/clia/regs/subpart_m.aspx

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. 
[bauer.ka...@mayo.edu]
Sent: Wednesday, August 01, 2012 1:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] MOHS Tech

Hi,

We are looking for MOHS tech and have an applicant that is not a
certified histotech.  Our MOHS techs are expected to gross tissues,
which requires a certified tech for high complexity testing... according
to CAP regulations.

I was wondering what other MOHS techs are doing that are not certified
technicians.  I would really like to give this person a chance, but am
afraid that I won't be able to because of the CAP standards.

Do anyone have any references, resources, or information that could help
me in this matter?

Thank you,

Karen

Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS
Lab Supervisor | Dermatology | Phone: 715-838-3205 |
bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street |
Eau Claire, WI 54702 | mayoclinichealthsystem.org
http://www.mayoclinichealthsystem.org/




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[Histonet] RE: EM send outs

[Walter Benton]

Try Hopkins or Univ of Maryland Medical Center.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Karlisch, Patricia 
[pkarli...@hmc.psu.edu]
Sent: Monday, July 23, 2012 2:13 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] EM send outs

All,
We are looking for a site to send our NEURO Electron Microscopic blocks for 
EM ONLY (Tech only).   There would be about 25-30 cases a year (muscle, nerve 
and possibly brain).  If you have information please let me know.  I would 
appreciate  it.


Patricia Karlisch

Supervisor, Histology
Department of Pathology and Laboratory Medicine
Penn State Milton S Hershey Medical Center
500 University Drive
Hershey, PA 17033-0850
Tel:  717-531-6072
Fax:  717-531-7741
Email: pkarli...@hmc.psu.edu

*E-Mail Confidentiality Notice*
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RE: [Histonet] Histology Cassettes - Large or Extra Large

[Walter Benton]

Cassettes used to process eyes should do the trick.
Fisher Scientific* Tissue Path* MACROSETTE* Processing/Embedding Cassettes

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of M.O. 
[modz9...@gmail.com]
Sent: Monday, April 23, 2012 6:40 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histology Cassettes - Large or Extra Large

Hello all, I am looking for extra large histology cassettes that will hold
specimens that are a max. 20mm thick (high).  Is there such a thing?  Where
would I find these larger cassettes?

Thank you,
Merissa
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RE: [Histonet] Pinning Specimen

[Walter Benton]

At previous institutions I purchased rolls or sheets of cork and that allowed 
us to pin and float samples like neck dissections, segments of colon etc I 
know Fisher sells the sheets and the roll of cork we purchased from an internet 
retailer. Just do a Google or Amazon search.

Making the paraffin blocks is easy as well. You can take any plastic container 
and pour off fresh paraffin or use paraffin from a tissue processor when you 
are disposing of it to make molds.

Hope this helps.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker 
[bakevicto...@gmail.com]
Sent: Thursday, April 19, 2012 9:16 AM
To: Heckford, Karen - SMMC-SF
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Pinning Specimen

Hi Karen -

Are yoru eferring to the boards that have the detachable mats?  I know they
still make them and the last one I heard of was through MOPEC.

I'm probably aging myself here, but we also used to make paraffin blocks
for large specimens and pin them flat for overnight fixation - it wasn't
pretty but it worked.

Hope this helps some.

Vikki

On Thu, Apr 19, 2012 at 8:03 AM, Heckford, Karen - SMMC-SF 
karen.heckf...@dignityhealth.org wrote:

 Does anyone know where to get specimen boards that you can pin specimens
 to and then submerse in formalin?  I ordered them a long time ago and
 cannot remember where I got them.

 Thanks,
 Karen Heckford HT ASCP CE
 Lead Histology Technician
 St. Mary's Medical Center
 450 Stanyan St.
 San Francisco, Ca. 94117
 415-668-1000 ext. 6167
 karen.heckf...@dignityhealth.org

Caution:  This email message, including all content and attachments,
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RE: [Histonet] Need the name of a piece of equipment - para trimmer

[Walter Benton]

http://www.thermoscientific.com/ecomm/servlet/productsdetail_11152_L10744_80555_12933395_-1

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles 
[estellamire...@gmail.com]
Sent: Thursday, March 29, 2012 12:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Need the name of a piece of equipment

Good day to all,
I need the name of the equipment that melts the wax off the edges of a
paraffin block.
Where can I purchase it.

Thank You
Stella
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RE: [Histonet] Woelcke Myelin Stain

[Walter Benton]

http://www.rowleybio.com/pdfs/K-684%20Woelcke's%20Method%20for%20Myelin%20Sheath.pdf

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks 
[esarri...@gmail.com]
Sent: Monday, November 28, 2011 1:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Woelcke Myelin Stain

Hi Histonet-

I hope everyone had a great Thanksgiving!  I was wondering if anyone has
any experience with the Woelcke Myelin Stain.  A researcher is trying to
use it to identify damaged neurons in brain sections.  I have found one
article with a rough procedure, but any other help anyone has would be
great!  Thanks!

Erin Sarricks


Erin Sarricks, HT (ASCP)

Histology Team Leader

USAMRICD Comparative Pathology Branch

Office: Bldg E-3081 Room 178

E-mail: erin.p.sarri...@us.army.mil

Phone: 410-436-1967
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[Histonet] RE: Validation

[Walter Benton]

Optimizing is getting the stain to look a certain way based on retrieval, 
incubations times etc validation is the process of running cases that are 
known positives of varying levels(degrees of positivity) and running them with 
normal cases to ensure the antibody is staining as intended based upon the 
optimized protocol that you have established. If for some reason the cases 
display background or do not stain the positive and negative cases as predicted 
based upon your optimization the antibody should not be validated as being 
ready for clinical use, it should be re-worked. Hope this helps.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie 
[amber.mcken...@gastrodocs.net]
Sent: Wednesday, November 16, 2011 1:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Validation

Just wondering what y'alls opinion is on validation:  I don't really understand 
why optimization isn't enough.  At that point, the pathologist has said what he 
wanted the stain to look like, so why do 3-10 positive slides on the new 
instrument to compare to previous slides from another instrument?  Thanks!

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RE: [Histonet] ?QIHC

[Walter Benton]

I think it is valuable since it allows you to learn things through study and 
application that you may not have been previously exposed to while preparing 
for the test. The test is strictly theory now versus the first year when 
stained slides were submitted and there was zero theory. Given the rapid 
changes in histology with ISH, IHC, IFs and other molecular based testing I 
don't see how it could hurt you to earn this credential. I'm not sure how pays 
is differentiated in the market for this qualification, but I do know that I 
see more and more jobs, especially those for IHC that require it or it is 
highly desirable for the position.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org 
[sbald...@mhhcc.org]
Sent: Tuesday, November 15, 2011 12:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ?QIHC

I WOULD LIKE TO KNOW ALSO

Thanks
Histology/Cytology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbald...@mhhcc.org
Ph 812-996-0210, 0216,  Fax 812-996-0232,
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
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[Histonet] RE: Chicago Sky Blue stain vs. KOH - striking comparison

[Walter Benton]

Here is what I found via a google search

http://www.sigmaaldrich.com/catalog/ProductDetail.do?D7=0N5=SEARCH_CONCAT_PNO%7CBRAND_KEYN4=C8679%7CSIGMAN25=0QS=ONF=SPEC

http://www.delasco.com/pcat/pdf/ChicagoBlue.pdf

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. 
[sandra.harris...@va.gov]
Sent: Thursday, November 10, 2011 12:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Chicago Sky Blue stain vs. KOH - striking comparison

Does anyone have ordering information for a stain called Chicago Sky
Blue(CSB)?  I just saw an article in the NOV issue of ASCP LABMEDICINE
(page 649) comparing KOH vs. CSB and, wow, is that a beautiful stain.



The results were dramatic, with about a 25-50%  increase in the
Dermatophytes identified and about a 5X increase in the Pityriasis
versicolor identification.



Thanks,



Sandy C. Harrison, HTL (ASCP)

Histology Supervisor

Minneapolis VA

612-467-2449



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RE: [Histonet] Standardizing staining/IHC protocols by case or tissue type

[Walter Benton]

Brandon,

In my experience there generally isn't a set SOP for ordering patterns. 
However, there are exceptions for breast cases, melanoma cases etc..., but 
those are the minority, not the majority. However, you can approach the cost 
effective process with them. Given most antibodies have a relatively long shelf 
life 2-3 years, I would recommend running a report and determining how many 
times an antibody was ordered of the course of year. In many cases as I 
experienced new antibodies replaced older antibodies and thus were not being 
ordered. As a result we removed those antibodies from the antibody offering. 
You'll need to reach an agreed upon number of tests that need to be ordered, 
which remove any bias that may result from presenting the numbers prior to that 
being discussed. In addition, it may be beneficial for you to work with you 
reference lab that performs IHC or Specials that you routinely do not perform 
to get firm TAT that will entice the pathologist to utilize that source instead 
of in-house. I hope this helps. We had to do this when I managed a large 
university lab with well over 125 antibodies. In the end we eliminated around 
20 or so and brought on 5-10 new antibodies that were being sent out frequently 
and ultimately were more cost effective in-house and had a better TAT in-house.

Hope this helps!

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pohl, Brandon J 
[bp...@swedishamerican.org]
Sent: Friday, October 07, 2011 1:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Standardizing staining/IHC protocols by case or tissue  
type

I am having trouble getting my pathologists to understand that their lack of 
standardization or consistency in ordering special stains and IHC is resulting 
in such low volumes that many of the stains or antibodies are not cost 
effective for us to run.  They seem to think that the way they each 
individually order certain stains based on preference or comfort level in 
reading is a norm amongst all pathologists.  I am hoping to get some feedback 
from anyone willing in order to find out if they are correct or if my point of 
standardizing their methods is valid.  For anyone willing to share, could you 
please let me know what types of protocols or panels you have established for 
different types of tissues or different types of cancers that are SOP for each 
of those cases.  I know some cases require flexibility and variation, but we 
have very little in terms of standardization that each pathologist follows.  
Thanks!


Brandon Pohl MAT, HTL (ASCP)
Histology Supervisor
Swedish American Hospital
Rockford, IL 61104
815-489-4279
bp...@swedishamerican.org
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RE: [Histonet] Equipment maintenance

[Walter Benton]

 DolbeyJamison
Tech One
 BelAir Instrument Company

are all good companies, just make sure they are trained for your specific 
pieces of equipment.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of zodia...@comcast.net 
[zodia...@comcast.net]
Sent: Thursday, September 29, 2011 12:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Equipment maintenance

To All,



I just wanted to see what companies other labs are using for the maintenance of 
their equipment(processor, embedder, microtomes...). We are located in south 
jersey.





Thanks for your help

Jenny



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[Histonet] RE: Microtome Repair

[Walter Benton]

 DolbeyJamison
 BelAir Instruments
Tech One

and of course the manufacturer of the unit.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wimer, Helen 
[wim...@si.edu]
Sent: Wednesday, July 27, 2011 9:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome Repair

 Can  anyone recommend  a company that does microtome repair in the Washington, 
DC/ Maryland area?  Thanks!

Helen F Wimer HT (ASCP)
Smithsonian Institution
Department of Vertebrate Zoology
Washington, DC
(301) 496-1391
wim...@si.edu
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[Histonet] RE: Remote Alarm for Processor

[Walter Benton]

You need to get an autodialer. Search for a company in your area. If you have 
Biomed or telecommunications in your facility they should be able to install it 
for you. I purchased a land line autodailer from Radio Shack years ago for 
three Sakura VIPs. VOIP autodialers are available as well.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara 
[sbree...@nmda.nmsu.edu]
Sent: Wednesday, July 13, 2011 9:14 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Remote Alarm for Processor

Do you use a remote alarm on your processor?  If so, would you tell me
what brand/model/manufacturer and how it works (keeping in mind that I
am Electronically Challenged)?  I run a Leica ASP300 in a new building
with only digital phone lines and zero cell phone reception inside the
building.  I can't think of anything else that would complicate this
issue more than that!  Any help out there?



Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)



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[Histonet] RE: Ventana's HER2 Dual ISH DNA Probe Cocktail

[Walter Benton]

In the past the FDA approval has been tied to running the antibody on the 
specific vendors' instrumentation using a predetermined protocol. This was true 
for Dako's EGFR that was FDA approved.

Ventana antibodies can be dispensed out of their vials and used on any 
instrumentation, however, you may have an IVD antibody at that point.

Definitely check around and see what others have to say.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne 
[lynne.b...@cvmc.org]
Sent: Monday, June 20, 2011 1:28 PM
To: Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Ventana's HER2 Dual ISH DNA Probe Cocktail

Ventana just received FDA approval for the first fully automated diagnostic 
assay for HER2 gene status determination in breast cancer patients.

I do not have a Ventana immunostainer and from what I have been told, it is a 
closed system and this new probe cocktail could not be used on another stainer, 
such as the Dako.

Is anyone out there in HistoLand using this new cocktail and am I correct that 
it would not work using the Dako Autostainer.

A Ventana rep may contact me with this information also.

Lynne Bell, HT (ASCP)
Histology Team Leader
Central Vermont Medical Center
Barre, Vermont  05641
802-371-4923


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[Histonet] RE: Source for p16 antibody...

[Walter Benton]

 MTM is the only company that can sell the antibody.
http://www.mtmlabs.com/us/index.php

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar 
[tmcne...@lmhealth.org]
Sent: Wednesday, June 15, 2011 1:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Source for p16 antibody...

Hello all,

I'm looking for p16 antibody for use on the Benchmark XT and was wondering what 
others are using and where they are getting it.  Any input appreciated.  Thanks.


Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org



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[Histonet] RE: Leica CV 5030

[Walter Benton]

 Azer Toluene Based Mountant with the 21 needle

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda 
[lbla...@digestivespecialists.com]
Sent: Thursday, June 09, 2011 10:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica CV 5030

Anyone using the Lieca CV 5030, what mounting medium and what size needle are 
you using?
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.commailto:lbla...@digestivespecialists.com

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RE: [Histonet] AP software

[Walter Benton]

Nicolle you may want to check into Vital Axis. It will handle the functions 
that you describe. They have a variety of options for pricing, which they can 
discuss with you. Marcus Cognetti is a good contact if you are interested. 
mcogne...@vitalaxis.com

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum 
[nic...@dlcjax.com]
Sent: Tuesday, June 07, 2011 2:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AP software

Hello Histonetters,

I need help. So I have used Pathlogix software for about 4yrs and I love
it. It so super simple and have never had any problems. Well, except for
one. The cost of leasing has increase by 19% each year for no apprearent
reason. We are now paying quarterly triple what we leased it for 4yrs ago.
The owner says cost are riseing due to the economy and prices are subject
to change without notice. Really! 19%. Cause im pretty sure thats bad
business and thats how you lose customer instead of keeping them. At any
rate, I need to find a new company. I have looked into a few systems but
the cost are hugh. We are a small derm lab with one reading physician and
im the only tech. So I do not need a large system. I need to be able to
generate path reports. Accession, create reports and logs for about 3000
specimen a year. I wish I could just purchase outright but will lease if
we have to.  I havent had must luck at finding software for small labs
except Pathlogix. Love the software, not so fond of the company. Please
tell me what software is out there and If you guys like it.

Thank a million,
Nicole Tatum HT ASCP


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[Histonet] RE: muscle bxs

[Walter Benton]

Poly Scientific sells this

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele 
[mmargio...@bmhmc.org]
Sent: Tuesday, March 22, 2011 11:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] muscle bxs

Hi All,

Quick question for those of you that do muscle biopsies.  Do you make up the 4% 
paraformaldehyde or buy it from a vendor.  If you make it up, would you share 
the recipe for it, please?  If you purchase it, which vendor do you use?

Thanks,
Michele
BMHMC



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RE: [Histonet] H-PAS and Modified Alcian Blue

[Walter Benton]

Probably a hyaluronidase PAS.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren 
[jaylundg...@gmail.com]
Sent: Monday, March 21, 2011 2:07 PM
To: Hannen, Valerie
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] H-PAS and Modified Alcian Blue

 Never heard of H-PAS, but a PASH is Periodic Acid - Schiff's with
Hematoxylin.  A PAS with a hematoxylin counterstain (NO eosin).

Sincerely,

Jay A. Lundgren M.S., HTL (ASCP)
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