[Histonet] open histology position
Temporary Histotechnologist Position in St. Paul, MN Interested in making some extra holiday cash? Regions Hospital is looking for an experienced histotechnologist to work a temporary, full time night shift through January 31, 2015. Regions Hospital is a Level I Adult and Pediatric trauma Center and teaching hospital serving Minnesota and western Wisconsin for more than 130 years. Regions is a private, non-profit hospital providing outstanding care in women's health, heart, cancer, surgery, orthopaedics, neurosciences, burn, emergency care, and more. Regions Hospital Lab is a state of the art. Regions is a part of the HealthPartners Family of Care. Additional information is available at regionshospital.com. Regions Hospital employees enjoy opportunities for personal and professional growth available only at one of the top teaching hospitals in the Twin Cities area. Our dedication to patient care and commitment to a healthy workplace, and Best Care, Best Experience, has allowed us to be recognized by the Minnesota Hospital Association with the Best Minnesota Hospital Workplace Award. This position will perform tests and procedures in our histology laboratory; perform functions associated with obtaining, receiving and processing of surgical pathology specimens in patients of all ages, to maintain laboratory equipment and supplies, and to perform related duties as assigned. Qualifications: Histotechnician board certification, ASCP or equivalent certification preferred. Two (2) years of Histology work experience within the last five (5) years may substitute for the educational qualifications. Education: Completion of an approved histology program and clinical histology internship or completion of an accredited two year laboratory technician training program in a vocational school or junior college. How to Apply: Apply online at www.regionshospital.comhttps://careers.peopleclick.com/careerscp/client_healthpartners/regions_external/jobDetails.do?functionName=getJobDetailjobPostId=59124localeCode=en-ushttp://www.regionshospital.com%3chttps:/careers.peopleclick.com/careerscp/client_healthpartners/regions_external/jobDetails.do?functionName=getJobDetailjobPostId=59124localeCode=en-us Additional Information We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. Dorothy Webb, HT (ASCP) 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] paraffin and Saturday work
We use Leica infiltration paraffin for the processors and their Blue Ribbon for embedding We work Saturdays to lighten the workload on Mondays or it is overwhelming. We are a 24/6 lab (no techs in lab Saturday after 3:30and until 0400 Mondays) Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] recycled formalin
How does everyone handle the use of recycled formalin in regard to tissue processors? OK to use on the processors? We have just recently installed the Creative Waste formalin recycler and I am looking for guidelines from those who have experience in this area. Much thanks, Dorothy Webb (Regions Histology, St. Paul, MN) This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] looking for TS replacement in Histology due to retirement
Histology Technical Specialist Position in St. Paul, MN Regions Hospital is a Level I Adult and Pediatric trauma Center and teaching hospital serving Minnesota and western Wisconsin for more than 130 years. Regions is a private, non-profit hospital providing outstanding care in women's health, heart, cancer, surgery, orthopaedics, neurosciences, burn, emergency care, and more. Regions Hospital Lab is a state of the art. Regions is a part of the HealthPartners Family of Care. Additional information is available at regionshospital.com Regions Hospital employees enjoy opportunities for personal and professional growth available only at one of the top teaching hospitals in the Twin Cities area. Our dedication to patient care and commitment to a healthy workplace, and Best Care, Best Experience, has allowed us to be recognized by the Minnesota Hospital Association with the Best Minnesota Hospital Workplace Award. This position will oversee the technical component of a work team in the histology laboratory including; implementation of methodologies, supply ordering and inventory management, interpretation of laboratory results for physicians and other hospital staff; performance of laboratory tests in the section assigned; assisting in performance evaluations; assisting in supervision of testing personnel; development and implementation of training and competency programs for histotechnicians and histology students interns, and to perform related duties as assigned. Qualifications: Graduation from an accredited college or university with an Associates (AA) or bachelor's degree in medical technology, histology, biology or related field; plus specialized training in laboratory science. A master's degree in related field may substitute for the above educational and registration requirements. Bachelor's degree preferred. At least four (4) years of experience as a Histotechnician or Histotechnologist or related field with at least two (2) years of experience in the specialty occurring within the last four years. How to Apply Apply online at www.regionshospital.comhttps://careers.peopleclick.com/careerscp/client_healthpartners/regions_external/jobDetails.do?functionName=getJobDetailjobPostId=59124localeCode=en-us Additional Information We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] questions
I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Prisma stainer
We have had the Prisma stainer for 2 years and LOVE it. Have never had a breakdown and it is a workhorse. It is user friendly and we do not have to use proprietary reagents, our choice! Dorothy Webb Regions Hospital Histology Technical Specialist -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, June 09, 2014 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: DAB away alternative (Dario Ricca) 2. G.I. biopsies and special stains (kathy.mach...@lpnt.net) 3. Re: G.I. biopsies and special stains (Michael Ann Jones) 4. RE: G.I. biopsies and special stains (Hannen, Valerie) 5. Re: G.I. biopsies and special stains (Sue) 6. RE: G.I. biopsies and special stains (Tom McNemar) 7. RE: G.I. biopsies and special stains (Boyd, Debbie M) 8. RE: G.I. biopsies and special stains (Mike Pence) 9. Re: RE: G.I. biopsies and special stains (Victoria Baker) 10. Re: G.I. biopsies and special stains (Michael Ann Jones) 11. Documenting special stain control blocks (Histology) 12. Special Stainers (Adesupo, Adesuyi (Banjo)) 13. FW:H E Stainers (Adesupo, Adesuyi (Banjo)) 14. Re: Special Stainers (Rene J Buesa) 15. RE: Special Stainers (Barbara Tibbs) 16. RE: Special Stainers (Cooper, Brian) -- Message: 1 Date: Mon, 9 Jun 2014 12:02:16 +0200 From: Dario Ricca ricca.da...@gmail.com Subject: Re: [Histonet] DAB away alternative To: histonet@lists.utsouthwestern.edu Message-ID: CAEods00W8tzEiOWhPdktUv=ns3hmnv8apkmmu4pquffjrhx...@mail.gmail.com Content-Type: text/plain; charset=UTF-8 Hi Thomas, I've see that Dako's DAB Away is composed of 1% Oxalic acid in water instead other DAB Aways (like the one you are using) are composed of sulfuric acid 10% in water. Dario 2014-06-06 17:53 GMT+02:00 Thomas Pier t...@medicine.wisc.edu: Hello Histonet, We have been using Lab Vision's DAB away to clean our Autostainer 360. However it seems a little wasteful to buy something that we can't use up before it expires. Do any of you have any exerpience with lower cost alternatives? Thanks, Tom ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Mon, 9 Jun 2014 08:14:42 -0500 From: kathy.mach...@lpnt.net Subject: [Histonet] G.I. biopsies and special stains To: histonet@lists.utsouthwestern.edu Message-ID: d24cd7725ad12d4ba0f0d7a60742b9b93b6b25b...@nadcwpmsgcms01.hca.corpad.net Content-Type: text/plain; charset=us-ascii I was wondering if other labs are automatically ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until HE is screened? Thanks! Danville Regional Medical Center Danville, VA kathy.mach...@lpnt.netmailto:kathy.mach...@lpnt.net 434-799-3868 -- Message: 3 Date: Mon, 9 Jun 2014 13:37:42 + From: Michael Ann Jones mjo...@metropath.com Subject: Re: [Histonet] G.I. biopsies and special stains To: kathy.mach...@lpnt.net kathy.mach...@lpnt.net, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: cfbb1562.744%mjo...@metropath.com Content-Type: text/plain; charset=us-ascii We order HP by IHC automatically on all stomach and GE juction specimens. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 6/9/14 7:14 AM, kathy.mach...@lpnt.net kathy.mach...@lpnt.net wrote: I was wondering if other labs are automatically ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until HE is screened? Thanks! Danville Regional Medical Center Danville, VA kathy.mach...@lpnt.netmailto:kathy.mach...@lpnt.net 434-799-3868 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 4 Date: Mon, 9 Jun 2014 09:42:23 -0400 From: Hannen, Valerie valerie.han...@parrishmed.com Subject: [Histonet] RE: G.I. biopsies and special stains To:
[Histonet] sign offs
We print a staining report from our IHC and/or special stain equipment and the tech initials he/she checked control and the pathologist initials it OK and returns the form of which we keep for 2 years. If it is a manual stain, we have a form we made to fill out and do the same with. For us, it was an area we were sited for several years ago at inspection and now cover it well. Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] controls
Does anyone out in histoland have a Candid control for fungus that you could spare? We are very much in need and would appreciate the help and see what we could possibly trade for. Much thanks and have a great week-end all! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] spill kits
You can go through any company pretty much, but remember you need a formalin spill kit as well as a solvent spill kit (for xylene and other spills) Dorothy Webb -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, January 07, 2014 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 122, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Spill kit (Amber McKenzie) -- Message: 1 Date: Tue, 7 Jan 2014 17:39:29 + From: Amber McKenzie amber.mcken...@gastrodocs.net Subject: [Histonet] Spill kit To: Vincent Rivera vriv...@westderm.com, 'Rathborne, Toni' trathbo...@somerset-healthcare.com, 'Eytalis, Robert A' robert-eyta...@riversidehealthcare.net, Anne Murvosh amurv...@advancederm.net, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 5a33c952bb67f4468af1f36d739212bc0112440...@jerry.gia.com Content-Type: text/plain; charset=us-ascii Where do you get your spill kit? Do you have a company and part number? Thanks! -Original Message- From: Vincent Rivera [mailto:vriv...@westderm.com] Sent: Tuesday, January 07, 2014 11:31 AM To: 'Rathborne, Toni'; 'Eytalis, Robert A'; Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Document, document, document! Believe me, you will get dinged on this if it is not documented, we found out first hand :( Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vriv...@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 07, 2014 8:09 AM To: 'Eytalis, Robert A'; Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Are they looking for documentation that it's been checked, or will they take your word for it? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert A Sent: Tuesday, January 07, 2014 10:51 AM To: Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Newest CAP inspection stupid thing. They want you to periodically check your spill kit for clumping, since it does not have an expiration date. I challenged it, but don't know if will go through. Robert A. Eytalis Laboratory Manager robert-eyta...@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Anne Murvosh [amurv...@advancederm.net] Sent: Tuesday, January 07, 2014 9:25 AM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Don't forget to make sure you have a spill kit and its labeled well with easy access, Inspectors will ask employees if they know where it and your fire extinguisher and alarm are located. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety items What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] Amyloid Red
Does anyone out there use the Amyloid Red stain from Anatech in place of the Congo Red dye? Due to the pricing, would like some critiques before I order it!!! Thanks, Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Congo red stain
We have been experiencing major difficulty with our Congo Red stain. We used to do it on the Artisan, but they changed raw products which has altered the stain and is not acceptable by our hematopathologists. Now we are back to manual and cannot seem to get it consistently acceptable. I would appreciate any ideas from the experts out there and if you want to send me offline your procedure, would be appreciated. Also, does anyone use a negative control , such as a keloid, on the slide? Thanks, as always, for your help! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryostat
Does anyone have any current information on the cryostats with UV for decontamination? Specifically, is it accepted as a means of decontamination by CAP or JS or any other regulatory agencies? Basically, is it worth the extra dollars to go that route? Much thanks and Happy 2014!! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need your expertise
We will finally be going green by recycling formalin. I would appreciate any advice or opinions on true recycling VS. a filtering type of system. As we all know, experience with a system or product is the best way to gain true knowledge! Also, I would appreciate knowing how others are handling extra levels of prostate needle bx's. We currently place them on charged slides and keep until the case is signed out, but it seems there should be a better way of handling this due to storage of the slides not to mention the extra cost involved! I know this is becoming more of a needed process with all of the RNA/DNA testing being done on diagnosed cancers. As always, I do appreciate any and all comments, advice, or words of wisdom!! Hope to see some of you in Providence late next week! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] meeting
Is anyone aware of a immunohistochemistry/molecular seminar and workshop that is held each February in Florida, I believe Boca Raton? Cannot find my info to get it on next years budget:( Thanks, Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] used xylene/VIP 6
We have the VIP 6 and really like it..user friendly and faster TAT. We have been having rotary valve concerns within our Leica Peloris tissue processor and the technician as well as Leica technical services both feel it is because we have been using recycled xylene in the cleaning cycle. I was always told that when recycling xylene, one gets a pure product back, which I always found hard to believe. We do use recycled xylene in routine tissue processing, rotating between new and recycled as we change the reagents and rotate. Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: samples
It is actually not a website but the internet communication (like a listserve) they have for PA’s. For instance, histology has one at histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu that is used to communicate and network online. Thanks again! Dorothy Webb From: sa...@royalmarker.com [mailto:sa...@royalmarker.com] Sent: Monday, June 10, 2013 12:20 PM To: Webb, Dorothy L Subject: RE: samples No problem, the samples are on the way. How were you recommended for our product? Could you let us know which website you are referring to? Thanks Again, Shawn Jenkins Original Message Subject: RE: samples From: Webb, Dorothy L dorothy.l.w...@healthpartners.commailto:dorothy.l.w...@healthpartners.com Date: Fri, June 07, 2013 1:40 pm To: 'sa...@royalmarker.commailto:sa...@royalmarker.com' sa...@royalmarker.commailto:sa...@royalmarker.com Thank you very much. Your marking inks were recommended on the website for PA’s. Your efficient response is greatly appreciated and we will get back to you next week with feedback! Dorothy Webb From: sa...@royalmarker.commailto:sa...@royalmarker.com [mailto:sa...@royalmarker.com] Sent: Friday, June 07, 2013 12:30 PM To: Webb, Dorothy L Subject: RE: samples Hello Dorothy, We will gladly send you a sample pack of our marking dyes to try. It will ship out today via UPS to the address below that you provided. I'll be sure to have your name as well as Jesse Spates on the label as well for a contact. The set will include 10 1/4oz bottles (all of our colors). Please contact us with any comments on the product. We appreciate your feedback and hope you are happy with our products. Have a great weekend and look forward to hearing from you. Best Regards, Shawn Jenkins Royal Marker (P) 954-462-0264 (F) 954-374-7290 Original Message Subject: samples From: Webb, Dorothy L dorothy.l.w...@healthpartners.commailto:dorothy.l.w...@healthpartners.com Date: Fri, June 07, 2013 11:59 am To: 'sa...@royalmarker.commailto:sa...@royalmarker.com' sa...@royalmarker.commailto:sa...@royalmarker.com Cc: Spates, Jesse D jesse.d.spa...@healthpartners.commailto:jesse.d.spa...@healthpartners.com Our PA’s are looking to try other inks than what we are currently using so we are wondering if we could please get some ink samples. Our hospital is Regions Hospital 640 Jackson St. St. Paul, MN. 55101 I am the technical specialist of histology, Dorothy Webb and the lead PA is Jesse Spates @ 651-254-4913. Thank you, Dorothy Webb Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CLIA
According to CLIA and CAP, IHC is considered High Complexity testing which is why some labs only allow a degreed tech to perform IHC tests. This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] brain tissue
How is everyone processing brain tissue from brain surgery, not necessarily a small bx? Does anyone have a separately timed process for such tissue to get them out faster? And what is the minimum fixation time suggested? Thanks, Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thyroid processing
Does anyone have some good tips to processing thyroid tissue from thyroidectomy specimens? There seems to be a fine line between underprocessing and overprocessing where the tissue becomes brittle. We currently process them on an overnite run where the tissue is in all reagents for 45 minutes. We have also tried a shorter run on the Peloris that takes about 6 hours. Would like to know what others are doing for more optimal tissue processing!! Thanks so much everyone! Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] question(s)
How does everyone handle storing extra paraffin sections that are cut as a standard on certain protocols, such as prostate needle bx's? We currently place them on a slide and save until the case is signed out. I am concerned with the amount of waste and cost with the way we are doing it and would like other ideas. If you keep the ribbons laid out on paper, do you stack the sheets of paper and is that problematic when it comes time to need that certain cut for IHC? Also, please let me know of those who have the VIP 6, how you like it, pros and cons and also the Leica ASP300S. Would greatly appreciate all feedback on both requests and thank you so very much!! Dorothy Webb , HT(ASCP) Regions Histology Technical Specialist This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC lead
What criteria does everyone use to hire for working in your IHC department? I am looking for feedback as to what qualifications are expected for IHC techs as well as qualifications and expectations of the lead in IHC. Also, what title do you have for the lead in IHC, technical specialist, coordinator, lead, etc??? Thanks ahead of time for your information! We are trying to reinvent the position and want to make it more like a community standard (community meaning the world of histology)!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 105, Issue 9
We make up a marking Eosin that we use on small bx before we place them in cassettes. We do not use actual teabags, but the mesh bags from Thermo Fisher that are not hard to pull apart and we never have anything sticking I the corners. I do know what you are referring to as some of the mesh bags ot teabags on the market are not mad of as fine material and are hard to pull apart and allow for specimens to gather in the corners. Our mesh bags are used for all specimens the PA's can pour into the bags. We also use the Obex papers for needle bx type specimens that can be laid out nicely on the paper and folded over the tissue once for optimal processing. On tissue processors, we are new this year to the Peloris processor from Leica and love the versatility of two retorts and the great reagent management which allows for less waste and less maintenance tech time. I was always a Sakura VIP fan previous, still am, but cannot compare the two retort option and how LEAN it has mad our lab! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, August 08, 2012 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: opinion on heating slides prior to IHC (Teri Johnson) 2. Manual for Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome? (Jennifer Johnson) 3. Re: Teabags (Bob Richmond) 4. RE: Re: Teabags (Sarah Dysart) 5. RE: Re: Teabags (Clouse, Rosanna) 6. Re: Re: Teabags (Paula Pierce) 7. RE: Re: Teabags (Shirley A. Powell) 8. tissue highlighting for visibility (cont...@histocare.com) 9. decalcification of premolar teeth (dog) (Alice Fraser) 10. National Society for Histotechnology Hard Tissue Forum Event - Bethesda, MD on August 18, 2012!!! (Jack Ratliff) 11. help ! paraffin section (Megha Kumar) 12. Re: tissue highlighting for visibility (Lee Peggy Wenk) 13. Re: Re: Teabags (Lee Peggy Wenk) 14. RE: tissue highlighting for visibility (MaryK Mendell) 15. Tissue Processor (Heckford, Karen - SMMC-SF) 16. RE: tissue highlighting for visibility (Vanessa Perez) 17. Re: Tissue Processor (Rene J Buesa) 18. Re: tissue highlighting for visibility (Rene J Buesa) 19. RE: decalcification of premolar teeth (dog) (Rittman, Barry R) 20. Re: help ! paraffin section (Rene J Buesa) -- Message: 1 Date: Tue, 7 Aug 2012 17:30:04 + From: Teri Johnson tjohn...@gnf.org Subject: [Histonet] Re: opinion on heating slides prior to IHC To: jefthomp...@salud.unm.edu jefthomp...@salud.unm.edu Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 9f3cfee76e51b64991c7485270890b400cdcb...@ex4.lj.gnf.org Content-Type: text/plain; charset=us-ascii Dear J, I agree with Rene Busa on his assessment that thawing and refreezing is very very bad for your epitopes. You don't do it with antibodies, you don't want to do it with your antigens either. Keep the slides you will not need frozen, taking out only what will be needed. Work quickly as exposure to the air will start the condensation process. I also agree that there does not need to be an ethanol rehydration step. That might be useful as a permeabilization step, but you only need to hydrate your tissue with buffer. You might not have realized that alcohol can permeabilize cells (even though they are already exposed through sectioning) and also affect the protein folding, so if your antibodies are already working using this scheme you might see a difference if you quit doing it. You also might not, so it could be useful to test it. As to whether to use heat to thaw, you can try putting the slides in front of fans at room temperature, or you can try fan forced ovens set at 25 or even 37 degrees if you are worried about heat. I have worked with a researcher who used unfixed cryosections of brain and put them in a 95 degree oven for 2 minutes prior to freezer storage. They were still able to get antibody and mRNA staining from their sample. I have also read accounts of people using a hair dryer (blow dryer) on the heat setting on them as well with no ill effect on their published studies. Who is to
[Histonet] DEEPER REQUESTS
Does anyone know if there is an average percentage that a histology lab should expect in deeper/recut requests? I am trying to establish if our pathologists order more than the norm or if I have a quality issue unfolding!! Also, do most labs use 2 controls for their pneumo staining, a routine fungus and a pneumo carnii positive? Thanks ahead of time! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] elastic stain
Why would an elastic stain work on a positive control, but the patient did not stain?? The patient tissue is from a temporal artery which should show some staining due to internal control?? Thanks ahead for any input on this puzzle! Dorothy Webb, HT (ASCP) This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryptosopridia
What stain is anyone using for cryptosporidia in histology?? I thought this was more of a microbiology test. Could use some help please!! Thanks, Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microtomy
We have run into an interesting scenario and wondering what the experts think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome. Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns. I had our service technician measure the microns and the equipment was cutting as set. I had the blocks cut on a different microtome and we have seen variations there also. My question is, does the amount of time on ice make a minor difference in the section thickness? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation! This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!! Thank you!! Dorothy Webb, HT (ASCP) This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] questions
For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc?? How long are unstained slides usable? Do any of you pick up extra sections from a ribbon if the tissue is minimal ? We do and have used them at times, but a pathologist would like them saved forever in case tissue is needed for a molecular test and there is not enough left in the block. Appreciate ahead of time your responses! Dorothy Webb, HT (ASCP) Regions Hospital This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NCCI policy update
Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC cocktail stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] gram controls
I remember giving a couple of chicken livers to micro and they incubated them overnight with gram +/-. I then cut those pieces smaller and processed them in separate cassettes. This process has owrked real well for us. Was in the Journal of Histotechnology but cannot remember which issue a few years back. Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decal
We use Rapid Cal Immuno (formic acid based) from BBC Biocemical out of Washington. Works great and does not compromise IHC as name suggests! Dorothy Webb -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Friday, September 09, 2011 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 94, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: P63 controls (Troutman, Kenneth A) 2. Full Time HT position in Oklahoma City (Gaiser, Marcia) 3. STP420 vs. Peloris (Davide Costanzo) 4. Seeking new position (Charles O) 5. Comment on HT/HTL (anuradha shrivastava) 6. Facebook (Nicole Tatum) 7. thinprep (tahs...@brain.net.pk) 8. I'm so sad for AFIP to close (Weems, Joyce) 9. RE: I'm so sad for AFIP to close (sdys...@mirnarx.com) 10. RE: I'm so sad for AFIP to close (Edwards, Richard E.) 11. Decal for IHC (Laudon, Heather M) 12. RE: I'm so sad for AFIP to close (Shirley A. Powell) 13. Floater Problem (Laurie Colbert) 14. Re: Decal for IHC (Rene J Buesa) 15. RE: Floater Problem (Bartlett, Jeanine (CDC/OID/NCEZID)) 16. RE: I'm so sad for AFIP to close (Kelly Cross) 17. Re: Floater Problem (Kelly Cross) 18. RE: Floater Problem (Bea DeBrosse-Serra) 19. RE: Decal for IHC (Shirley A. Powell) 20. Traveling HistoTech San Francisco Area (Heckford, Karen - SMMC-SF) 21. RE: Floater Problem (Shirley A. Powell) 22. RE: Decal for IHC (Sebree Linda A) -- Message: 1 Date: Thu, 8 Sep 2011 14:16:21 -0500 From: Troutman, Kenneth A ashley.trout...@vanderbilt.edu Subject: Re: [Histonet] P63 controls To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: 7b310892042da74cb3590053f424cfe61441fce...@its-hcwnem06.ds.vanderbilt.edu Content-Type: text/plain; charset=us-ascii We use normal prostate and we have also used skin in the past-they both work well. I would avoid pre-cutting a lot of controls, though; it has a tendency to loose antigenicity. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 9 Date: Thu, 8 Sep 2011 09:08:52 -0400 From: Sara Baldwin/mhhcc.org sbald...@mhhcc.org Subject: [Histonet] P63 controls To: histonet@lists.utsouthwestern.edu Message-ID: OF62E3D6D8.5113CF5C-ON85257905.0048390E-85257905.00483911@LocalDomain Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters We are having trouble with our control for P63 what is everyone using? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. -- Message: 2 Date: Thu, 8 Sep 2011 15:16:48 -0500 From: Gaiser, Marcia marcia_gai...@ssmhc.com Subject: [Histonet] Full Time HT position in Oklahoma City To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 728f817c02110e498d803a7c3b0c62480690a37...@s009-apexm06.ds.ad.ssmhc.com Content-Type: text/plain; charset=Windows-1252 POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION, LICENSURE, BONDING: Certified as an HT or HLT by the American Society of Clinical Pathologists (ASCP) ? or ? other nationally recognized certifying agency acceptable to the Laboratory Director ? or ? experience acceptable to the Laboratory Director. EXPERIENCE: Two years of satisfactory histology experience. SIGN ON BONUS AVAILABLE FOR QUALIFIED CANDIDATES. Outstanding benefits package including generous paid time off. Apply online at www.saintsok.comhttp://www.saintsok.comhttp://www.saintsok.com/https://mobile.ssmhc.com/owa/UrlBlockedError.aspx, Ad# 10762, or contact Christina Hughes at (405) 272-6105 for more information. Thank you! Christina Hughes HR Recruiter St. Anthony Hospital (405) 272-6105 - phone (405) 272-6781 - fax Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you
[Histonet] Recycled alcohol
You do not get 100% alcohol back from recycling, so it cannot be used as such in either the processor or stainer. Basically, you have to use it as 95%. That may be your problem!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] bluing
Looking to change my bluing step in the HE process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] blades
Trying to clean up some things hanging out there in our lab and wondering what everyone does with a blade that has been used minimally and tech done for the day with the microtome. Where do you store that blade for use tomorrow or do you toss and not worry about the cost involved? I do not like them sitting on top of the microtome. Any good ideas?? Thanks, as always! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Von kossa stain
What is everyone using for their light when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] QC documentation
How does everyone handle their QC documentation on special stains and IHC? We currently print out the run information from our stainer(s) and have the tech initial for her QC and the pathologist sign after they review the slides. I am hoping that someone has a way of doing this electronically. I hope to learn of a better way to save some trees!!! Thank you ahead of time! Dorothy Webb, HT Regions Histology Technical Specialist This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave processing
Does anyone know what the compliance is, if any, with JACHO or CAP regarding the pathologist doing a paper QC on each microwave run? We have been having them sign a QC sheet that we hand in with each microwave run and our looking at ways to rid our area of some of the unnecessary paperwork! Thanks! Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Whole mount histology
I am totally unfamiliar regarding whole mount histology as it pertains to prostate histology. Can anyone in histoland assist me in finding information out regarding more specifics, especially in lieu of what costs would be incurred to implement this in a routine hospital histology department. Appreciate any and all guidance!! Dorothy Webb, HT Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Parathyroid staining
Does anyone use Toluidine blue to stain parathyroids when they come in fresh for a frozen section? One of our pathologist heard of this and I am not familiar with it. I have heard of it with the Paragon stain, but not certain what percentage would be needed if only Toluidine blue was used. Thanks ahead of time and Happy Holidays!! Dorothy Webb, HT This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Physician signature
According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] dishwasher
We recently remodeled our histology lab (YAY) and have obtained a dishwasher for our use. Would those of you who presently are using a dishwasher share what detergent you use and where you order it from? We plan to do our staining dishes, both HE and coplin jars from hand special stains. Any helpful hints would be appreciated and thanks ahead of time!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cleaning molds
Does anyone in histoland clean their embedding molds in a dishwasher? Otherwise, besides placing in the cleaning cycle of your processor, how do sites clean their molds?? Simple, but plaguing question!!! Thanks all! Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome alignment
We recently purchased the new alignment tool from Source medical products that works on any microtome as it uses a leveling bubble. It is round, so it makes certain all is level from any direction. Works well and has helped us out. I have staff use it daily as it takes minimal time. Only $200. Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decalcification
I would appreciate any feedback on what all are using in your decalcification process. We get a lot of large bones in and the past 2-3 months have noticed a huge problem in our microtomy process with these samples. We have been grossing the bones in and leaving the sample in the cassette in 10% formalin for 24 hours befoere placing in decal for up to 8 hours and still having the inner portion of the sample look underprocessed and crunchy! Any suggestions would be appreciated! Dorothy Webb, HT Regions Hospital Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ER and PR validation
In a recent article written by 4 pathologists, including Dr. Elizabeth Hammon and Dr. Patrick Fitzgibbons, the recommendations are now including testing with a lab that has validated it's assay against clinical outcomes. How is everyone planning on fulfilling this requirement inasmuch as we are not aware of any lab in our metro area with this kind of compliance. Thanks, as always, for any guidance in this area! If you would like a reprint of this article, email is pf...@stjoe.org and the article is entitle Recommendations for Validating Estrogen and Progesterone Receptor immunohistochemistry Assays. Dorothy Webb, HT Regions Histology Technical Supervisor This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE stainers
We are in the market for a new HE automated stainer and am hoping I get some feedback on this request! I wold like to hear from the techs who work with either the Prisma from Sakura or the Leica auto or multi stainers, PLEASE!! You can email me directly if you would like your comments private at dorothy.l.w...@healthpartners.com Thanks much ahead of time!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hpylori
Does anyone have an abundance of Hpylori tissue in blocks they would be willing to part with or trade for another control block? We are running very low and haven't had a positive for a while!! Much appreciated!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lot numbers
Would anyone be willing to share with me how you record lot numbers of all reagents and histology components as they are used? I have a couple of ideas on how to capture this, but, they seem too detailed!! Obviously, our IHC and special stains done on the automated stainer are documented by the equipment, but, looking at all the hand stains, HE's, processors, etc.,etc!! Thank you for any guidance in this area!! Dorothy Webb, HT Regions Histology Technical Superviosr 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dimethyl sulfoxide
We purchase ours from Sigma-Aldrich. 1-800-558-9160 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sally
A big THANK YOU AND JOB WELL DONE to Sally Breeden for compiling the stories of histotechs and how they got their start! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] questions
We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in LD for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing. Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain? Thank you ahead of time for any and all responses!! Dorothy Webb, HT (ASCP) Regions Histology Lab This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] EDTA
I am looking into the various decals on the market and have found one that in addition to formic acid, has EDTA in the mix. I have never worked with EDTA so would appre ciate any help in your comments on the use of EDTA in decalcification methods for bone marrow and routine specimens. Thank you ahead of time for your advice! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job opening
Regions Hospital is a full-service, private hospital and a Level I Adult and Pediatric trauma center. Employees enjoy opportunities for personal and professional growth available only at one of the top teaching hospitals in the Twin Cities area. Our dedication to patient care and commitment to a healthy workplace, and Best Care, Best Experience, has allowed us to be recognized by the Minnesota Hospital Association with the Best Minnesota Hospital Workplace Award. Regions Hospital Histology Department is currently seeking professional, self-motivated, quality and team-focused Histotechnicians to join our team. As our ideal candidate, you are HT ASCP certified or eligible and have 1 year of hospital laboratory experience as a Histotechnician. As a Histotechnician at Regions Hospital, you will join an innovative and collaborative group of professionals in a supportive work environment. You will work in a department that has 13 Pathologists, uses barcoding technology, performs special and immunohistochemistry staining, and is embarking on a laboratory expansion. You will support Regions Hospital and HealthPartners network of clinics processing all types of surgical pathology specimens. As part of our team, you will share in the responsibility for quality assurance and participate in continuing education projects for the department. The Twin Cities is the fastest growing urban center in the Midwest. Living here you'll enjoy one of the nation's most economically robust regions, situated among scenic forests, rivers, lakes, parks, and golf courses. The Twin Cities region is home of two thriving downtowns, internationally acclaimed orchestras and museums, and five major league sports teams. For consideration, please apply at www.regionshospital.comhttp://www.regionshospital.com under career opportunities Job ID #'s: 16358, 16360, 16376 -EOE Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Special stains
We are switching automated special stain equipment to a different vendor and am wondering if we have to validate all of the special stains we do. I am from the old school and the validation process, other than IHC, is not my forte!! Any advice would be appreciated, so, thanks ahead of time!! Dorothy Webb, Ht (ASCP) Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Negative control
We have not done negative controls, but, have recently started placing both a negative and positive control on slides for Congo Red based on recommendation by Mayo. We are using a keloid scar. They state that on consult cases, they have often noticed overstaining on high collagen cases. Our pathologists thought it would be good for comparison! But, that is the only one for now!! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dragon
Our pathology department has been using Dragon for 3 years now and really like it, both PA's and pathologists alike! It is time saving and easy to adapt to, even the Pathologists who have their foreign accents!! In fact, our entire facility is switching to Dragon for dictation. The only negative was the loss of FTE's for secretaries, as it took their work away!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Confirmation of reports
We use Copath for Histology LIS and Epic as our Hospital and clinic based system. In Epic, you can retrieve who reviewed your results by looking at the result in Chart Review. Look below the results, it lists who and when. There is also an electronic trail that Epic can produce if needed. Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ISH
Can anyone supply me with a vendor for Kappa/Lambda ISH? I heard that Dako and Ventana have had to take their ASR Kappa.Lamda product off of the market. I am in need of where to go for ASR or IVD in this area!! Thanks, Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozen control
I am resubmitting my question concerning the use of a control for the frozen section staining. Does anyone run a control and, if so, how often and do you store them in the freezer? This was a suggestion we received, but, not certain if we need or want to go this route. Any input would be greatly appreciated and thanks so much! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozen controls
Does anyone run a control for your frozen section staining set-up? If so, how often do you run a control and do you store them in the freezer? Thanks for your answers!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alk-1 controls
Does anyone have a good source to purchase ALK-1 IHC controls from? We were getting them from Cell Marque, but their source is no longer available!! Thanks for any help!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RPMI
What is the correct way to store RPMI after placing a tissue sample in it, refrigerated or ambient? Thanks for your educated assistance!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] General data cassette printer
Is anyone in Histoland using the General Data cassette printer or trying it out?? If so, please let me know your thoughts on the printer and process!! Much appreciated!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Cervical cancer mission in Peru
From: Savaloja, Lynnette C Sent: Thursday, April 02, 2009 11:06 AM To: Webb, Dorothy L Cc: Semerad, Shelly A Subject: FW: Cervical cancer mission in Peru Importance: High Hey Dorothy, Got an old microtome laying around (see below)? Let me know... L ;) From: Fischer, Andrew [mailto:andrew.fisc...@umassmemorial.org] Sent: Thursday, April 02, 2009 10:58 AM To: memb...@lists.cytopathology.org Subject: [ASC Listserv] Cervical cancer mission in Peru Dear ASC members, Does anyone have a working microtome that could be donated to the INCCA (International Cervical Cancer Foundation) cervical cancer screening and treatment mission in Peru? This important program does not yet have a microtome to allow evaluation of the biopsies/cones. We will need it by the end of May. Please contact me if you can help. For more information on the Peru mission, see http://www.theincca.org/indextest2.html Sincerely, Andy Fischer UMASS The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, transmission, re-transmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and delete the material from any computer. This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone specimens
Does your facility require bone from a total hip or knee replacements from patients with osteoarthritis or Degenerative Joint Disease to be sent to pathology, or are these samples exempt? If they are sent to pathology, do they perform a gross only or gross+micro. Thank You, Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] specimens
The first problem was with the tech who removed thespecimens from the OR without checking to make certain everything was included. She/he should never have left the OR without responding to the issue and that would have taken the blame off of the histotechs. We have OR bring specimens to us and sign in with a label as used on the container, initial and date/time everything. The techs accepting specimens have to sign off and accept responsibility at that point. When we did go to the OR, a few yaers ago, we had the same logbook in place there. The OR personnel had to sign everything in and when we picked the tissue up, we checked each specimen off in the logbook with our initials and time/date. Same process, only in reverse. Once anyone initialed the logbook, it was that person's responsibility as to the specimen integrity. Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Flammable storage
How does everyone store any reagents that need refrigeration that are also flammable? We have always just stored them in the routine histology refrigerator. I have never had this concern come up before and the charge tech of toxicology is now questioning this type of storage. Any guidance on this would be greatly appreciated!! Thanks, as always fellow histonetters, the best group in the world!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Patient identifier
I never saw the posting for this, so, am resending my response!! __ From: Webb, Dorothy L Sent: Thursday, January 29, 2009 8:08 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Patient identifier According to the rule regarding 2 patient identifiers, it is our understanding that the 2 identifiers must be on the container label and in the computer system upon accessioning. When the computer assigns the surgical case number, all info is linked to that listed surgical accessioned number and your 2 or more identifiers are pulled up with the case. That also keeps within HIPPA guidelines to have patient information openly available when blocks or slides are sent out or left out in view. Otherwise, barcoding is the ideal way to place, view, and tie all information needed!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Patient identifier
According to the rule regarding 2 patient identifiers, it is our understanding that the 2 identifiers must be on the container label and in the computer system upon accessioning. When the computer assigns the surgical case number, all info is linked to that listed surgical accessioned number and your 2 or more identifiers are pulled up with the case. That also keeps within HIPPA guidelines to have patient information openly available when blocks or slides are sent out or left out in view. Otherwise, barcoding is the ideal way to place, view, and tie all information needed!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome alignment
We use the microtome alignment instrument, which can be purchased from several companies. It is in our weekly checklist that all microtomes have to be adjusted (checked) with the microtome aligner. All microtomes are cut at the same angle also! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Silanized slides
Does anyone have any idea of how long silanized slides made in lab are good for? Would it be the same outdate as is on the solution one is using to prepare the silanized slides? Thanks, as always!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control tissue
The best control is healthy skin with the connective tissue in place but a tonsil is also great for checking how well your lymph tissue is staining. Tonsil should be cut thinner just like the lymph nodes so you should use the tonsil when staining lymph nodes. Make sure the tonsil has connective tissue in it so you can see the eosin staining in the collagen, blood vessels, cytoplasm, and RBC's. Another good tissue is cervix with the glands. The glandular area gives you the nice hematoxylin staining and the muscle and collagen fibers give you a nice contrast on the eosin staining plus you can always get cervix. I would stay away from the appendix since there is usually a lot of necrosis and the placenta is a little to bloody so the eosin is always some what overwhelming for a good evaluation. This is information I was given when posing the question years ago!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Can you believe it
Has anyone ever heard of cassettes and tissue totally disintegrating in formic acid decal solution with a high heat on the solution for about 3 hours? We had 3 cassettes with tissue for decal placed in our container on the platform for adding agitation. Someone had moved the knob to heat and this wasn't noticed until about 2-3 hours later, when it was boiling! We cannot locate the cassettes (I think someone misplaced them) and others think they totally disintegrated with the decal and heat...what does everyone think? In all of my years of histology, never have I heard of anything so weird .. Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fatty tissue
I would like to know how other labs are processing fatty tissue, especially breast for optimal processing? We have been doing a few reprocessing of these specimens and my pathologist feels this should not be happening! Yes, the PA's are, at times, cutting the sections larger than 3 mm thick, but, at times the tumor is ingrained in the fatty area and they need to obtain as much of the perimeter as they can. Does anyone agitate or heat fixate their specimens prior to processing? Would appreciate any feedback on this age old dilemma. Also, I only heard back from one person on the recycling issue. Any input would be appreciated as to savings incurred and a still vs. recycler. This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] recycling
We reycle xylene presently and are looking into recycling alcohol and formalin, mostly to look for a costs savings. For those of you in histoland that recycle those products, how generous are your cost savings? I am in doubt as to the fact that you cannot get 100% recycled alcohol as a byproduct of ones' recycling. I still will need to purchase absolute alcohol and that is the majority of alcohol ordered. Also, are the aldehydes and buffers costly that one needs to replenish in recycled formalin? And, has anyone seen any problems with their processing or staining when using these recycled products or do you find the same, optimal slide production?? Thanks much for sharing your experiences!! Dorothy Webb Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] validation
Is it better to use a lab's own tissue for validation in a microarray for Ihc purposes or would it be passable to use TMA's from another source?? Thanks for your opinions!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Amyloid
We have been cutting our tissue for amyloid staining @ 8 microns. One of my pathologists heard that 10 microns is now standard and to use a negative and weakly positive control. Does anyone have any new information in this area? Thanks ahead of time! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
