[Histonet] AFB and Spirochetes slides needed

[Sheeder, Christopher via Histonet]

Good morning all,

I am validating new stains and looking for 4-5 slides each of AFB+ and 
Spirochetes+.

I am willing to trade for something you need.

Email me directly if you want to work something out.

Regards-

Christopher Sheeder, HT(ASCP)CMQIHC
Department of Laboratories
Seattle Children's Hospital
4800 Sand Point Way NE
Seattle, WA 98105

Office: 206-987-6259

christopher.shee...@seattlechildrens.org

WWW  seattlechildrens.org
COMPASSION | EXCELLENCE | INTEGRITY | COLLABORATION | EQUITY | INNOVATION

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[Histonet] AFB controls

[Charles Riley via Histonet]

Does anyone have any control tissue that they can spare for AFB staining?   I 
am willing to trade for any other controls tissues that you may need in return.
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[Histonet] AFB control and Recycling formalin

[Nancy Schmitt via Histonet]

Hello-

  1.  Does anyone have extra AFB control that we could obtain some from?


  1.  Recycling formalin:  if you are recycling formalin, do you recycle for 
any other sites?



 *   How do you charge for this?
 *   Do you charge for what is coming in  or what is going out?
Working through a multi-site dissolution and trying to future plan..
Thoughts appreciated!

Nancy Schmitt HT, MLT(ASCP)
Pathology Support Services Manager
United Clinical Laboratories
250 Mercy Drive
Dubuque, IA  52001
Ph 563-589-9810

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[Histonet] AFB blocks

[Ken M via Histonet]

Does anyone know where I can obtain good Acid Fast Bacteria tissue blocks?

Ken
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Re: [Histonet] AFB STAIN

[WILLIAM DESALVO via Histonet]

I completely agree with Tony. Not likely to see any sloughing off (shedding) of 
organisms from FFPE tissue blocks.

William DeSalvo


From: Tony Henwood (SCHN) via Histonet 
Sent: Thursday, October 4, 2018 1:54 PM
To: adesupo2...@hotmail.com; Laurie Colbert
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] AFB STAIN

Cross-contamination has not been proven in FFPE tissues.

I have not seen it in nearly 40 years of practice

Do you have any evidence of cross-contamination in FFPE?

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


From: Laurie Colbert via Histonet 
Sent: Thursday, October 4, 2018 10:36 PM
To: adesupo2...@hotmail.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] AFB STAIN

You should put the AFB control on a separate slide to prevent 
cross-contamination.

Laurie Redmond


-Original Message-
From: ADESUPO ADESUYI via Histonet 
To: histonet 
Sent: Wed, Oct 3, 2018 7:58 pm
Subject: [Histonet] AFB STAIN

Hello,
I have a question please. For the AFB Stain, do we put both the control tissue 
and the patient on the same slide?

Thanks,
Ade
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Re: [Histonet] AFB STAIN

[Tony Henwood (SCHN) via Histonet]

Cross-contamination has not been proven in FFPE tissues.

I have not seen it in nearly 40 years of practice

Do you have any evidence of cross-contamination in FFPE?

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


From: Laurie Colbert via Histonet 
Sent: Thursday, October 4, 2018 10:36 PM
To: adesupo2...@hotmail.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] AFB STAIN

You should put the AFB control on a separate slide to prevent 
cross-contamination.

Laurie Redmond


-Original Message-
From: ADESUPO ADESUYI via Histonet 
To: histonet 
Sent: Wed, Oct 3, 2018 7:58 pm
Subject: [Histonet] AFB STAIN

Hello,
I have a question please. For the AFB Stain, do we put both the control tissue 
and the patient on the same slide?

Thanks,
Ade
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Re: [Histonet] AFB STAIN

[Cristi Rigazio via Histonet]

We always try to put them in the same slide, but sometimes it doesn’t work out 
the way.  Either would be acceptable.

Sent from my iPhone

> On Oct 4, 2018, at 12:35 AM, Tony Henwood (SCHN) via Histonet 
>  wrote:
> 
> We do,
> 
> It works well
> 
> 
> Regards 
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
> Principal Scientist, the Children's Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney 
> Tel: 612 9845 3306 
> Fax: 612 9845 3318 
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 
> 
> 
> -Original Message-
> From: ADESUPO ADESUYI via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
> Sent: Thursday, 4 October 2018 12:54 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] AFB STAIN
> 
> Hello,
> I have a question please. For the AFB Stain, do we put both the 
> control tissue and the patient on the same slide?
> 
> Thanks,
> Ade
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> notify the sender.
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> 
> 
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Re: [Histonet] AFB STAIN

[Tony Henwood (SCHN) via Histonet]

We do,

It works well


Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: ADESUPO ADESUYI via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, 4 October 2018 12:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AFB STAIN

Hello,
 I have a question please. For the AFB Stain, do we put both the 
control tissue and the patient on the same slide?

Thanks,
Ade
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[Histonet] AFB STAIN

[ADESUPO ADESUYI via Histonet]

Hello,
 I have a question please. For the AFB Stain, do we put both the 
control tissue and the patient on the same slide?

Thanks,
Ade
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Re: [Histonet] AFB Stain on Cytospin Preps

[deGuzman, Jose R via Histonet]

Greg,

I'm not sure if anyone has responded to your question, the waxy coat of 
acid-fast bacilli is sensitive to xylene and alcohol not to acid. Carbol 
Fuchsin is soluble in acid alcohol but if the waxy coat of the bacteria is 
intact, it will hold the stain. What I've suggested before is a cyto-spin 
preparation of unfixed fluid avoiding any alcohol or xylene even for 
dehydration and clearing.

Hope this helps.

J


Good day colleagues,
We are occasionally being asked to do an acid-fast stain (Ziehl-Neelsen) on a 
alcohol-fixed cytospin preparation from a cytology fluid.

Typically in Cytology, if a specimen is very bloody, we will add acetic acid to 
lyse the red cells. Does anyone know if acetic acid would have any adverse 
affect on the stainability of AFB organisms if present?

Thank you,
Greg

--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!*

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[Histonet] AFB Stain on Cytospin Preps

[Greg Dobbin via Histonet]

Good day colleagues,
We are occasionally being asked to do an acid-fast stain (Ziehl-Neelsen) on
a alcohol-fixed cytospin preparation from a cytology fluid.

Typically in Cytology, if a specimen is very bloody, we will add acetic
acid to lyse the red cells. Does anyone know if acetic acid would have any
adverse affect on the stainability of AFB organisms if present?

Thank you,
Greg

-- 
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!*
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[Histonet] AFB Control Blocks

[Clarence Owens via Histonet]

Good Morning All,

I am in search for 2 blocks of AFB positive lung blocks. Does anyone have 2
blocks they could spare. I am willing to trade a control block for another
control block. I thank you for your help in advance.

Regards,

- Clarence Owens
​, HT (ASCP) QIHC​
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[Histonet] AFB Stain

[Adesupo, Adesuyi (Banjo) via Histonet]

Hi,
I want to know whether it is compulsory to use two controls (positive & 
negative) for AFB Stain.

Thanks,
Banjo Adesuyi
==
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Re: [Histonet] AFB Control Question

[Mayer,Toysha N via Histonet]

Pam,
I worked with our microbiology department and had them to obtain some positive 
organisms and with our surgery and morgue for fresh tissue.  We then inoculate 
the tissue and process it.  Positive blocks are kept on hand and used.  We also 
pass a copy of the slides back to the microbiology department to keep on file 
as a good batch.  It seems to work for us in the educational setting.  We have 
done afb, gms, and gram.  Our next batch will include Cryptococcus, and h. 
pylori.
Hope that helps!

Sincerely,

Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481
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Message: 2
Date: Wed, 15 Feb 2017 15:53:07 +
From: "Marcum, Pamela A" <pamar...@uams.edu>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] AFB Control Question
Message-ID: <8c03b2f73b8e4e5593bf93faa4069...@mail13m2n1.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"

Good morning,

We are attempting to find AFB controls from human sources.  We would trade if 
we have something you need and we have available.  Does anyone have blocks they 
might share or trade without upsetting our HIPPA people on either side.  I am 
really not interested in buying slides as we cut larger blocks down and have 
controls on with the patient tissue.  Also the control slides are general in 
the middle of the slide and force to use two slides for every test (too many 
ordering and all want a control) which makes the cost prohibitive.  Our 
pathologists prefer human tissue and most of the companies I talk to either 
don't know or won't say it is animal origin or human.


--
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[Histonet] AFB Control

[Forest Blankenship via Histonet]

Hello Pam,

It is always recommended to have AFB control and patient tissues run separately 
in parallel to avoid contamination from the control to the patient tissue. The 
most economical way to do this is to use a staining tray and use a pipette to 
do all the steps involved then it is impossible for the bacteria to transfer to 
the patient slide.

Thank you,
Forest K. Blankenship, HTL(ASCP)
Histology Manager
Driscoll Childrens Hospital
Corpus Christi, TX

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[Histonet] AFB Control Question

[Marcum, Pamela A via Histonet]

Good morning,

We are attempting to find AFB controls from human sources.  We would trade if 
we have something you need and we have available.  Does anyone have blocks they 
might share or trade without upsetting our HIPPA people on either side.  I am 
really not interested in buying slides as we cut larger blocks down and have 
controls on with the patient tissue.  Also the control slides are general in 
the middle of the slide and force to use two slides for every test (too many 
ordering and all want a control) which makes the cost prohibitive.  Our 
pathologists prefer human tissue and most of the companies I talk to either 
don't know or won't say it is animal origin or human.


--
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Re: [Histonet] AFB contaminant - OR - Monkish Preparation

[Monson, Frederick via Histonet]

I slways filtered my air (5um) and my water (0.22um).  Only tap water rinses 
for Hemnatoxylin, Schiff's and other rinses were not rinsed.  All of my MolBio 
and Immuno- stuff was done with filtered (clean?) water at least.

The rule is:  be Monkish at the bench and as messy as you like in your bedroom 
(if you are a male and only until you are married),

Cheers,

Fred 

Inside your water supply, lurking behind the water fawcet, is a pipe who's 
surface is lined with a BIO-film.
Further horrors await the scientist who fixes and studies the washer in the 
fawcet whose seal is the better for the 'FILM-m.'
Evolution provides variation, and every new flower that grows provides a new 
isolated environment for speciation.
My alimentary biome is unique as we are all unique.  Yukky, but true.

Frederick C Monson, PhD
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of Pannsylvania
West Chester, PA, 1938
610-738-0437
fmon...@wcupa.edu

From: Abbott, Tanya tanyaabb...@catholichealth.net
Sent: Thursday, July 9, 2015 3:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AFB contaminant

Has anyone ever had a problem with a possible contaminant in their AFB hand 
stain? If so, how did you deal with it? Any suggestions?
Ours actually looked like an AFB like organism, which we later determined to be 
negative.
Thanks!

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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Re: [Histonet] AFB contaminant

[Morken, Timothy]

We had some a few years ago and thought it was contaminating bacteria, but 
turned out to be dirty water from the di tap. We had our plumbers take it apart 
and clean everything. 


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-Original Message-
From: Abbott, Tanya [mailto:tanyaabb...@catholichealth.net] 
Sent: Thursday, July 09, 2015 12:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AFB contaminant

Has anyone ever had a problem with a possible contaminant in their AFB hand 
stain? If so, how did you deal with it? Any suggestions?
Ours actually looked like an AFB like organism, which we later determined to be 
negative.
Thanks!

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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[Histonet] AFB contaminant

[Abbott, Tanya]

Has anyone ever had a problem with a possible contaminant in their AFB hand 
stain? If so, how did you deal with it? Any suggestions?
Ours actually looked like an AFB like organism, which we later determined to be 
negative.
Thanks!

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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Re: [Histonet] AFB contaminant

[Bryan Llewellyn]

Hi,
This may be AFB contamination from tap water. The organisms grow in the 
end of cold water taps. You should take the taps apart and thoroughly 
descale and scrub out. The organisms may be somewhat longer and thicker 
than TB, and may be quite distinctly beaded.


Bryan Llewellyn

Abbott, Tanya wrote:

Has anyone ever had a problem with a possible contaminant in their AFB hand 
stain? If so, how did you deal with it? Any suggestions?
Ours actually looked like an AFB like organism, which we later determined to be 
negative.
Thanks!

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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Re: [Histonet] AFB contaminant

[Kienitz, Kari]

most likely a fungus ball, confirm with a PAS


Kari Kienitz HT, (ASCP)
Histology Laboratory
Gastroenterology-EAST
The Oregon Clinic
 NE 99th Ave
Portland, OR  97220
503.935.8311
kkien...@orclinic.com




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From: Abbott, Tanya [tanyaabb...@catholichealth.net]
Sent: Thursday, July 09, 2015 12:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AFB contaminant

Has anyone ever had a problem with a possible contaminant in their AFB hand 
stain? If so, how did you deal with it? Any suggestions?
Ours actually looked like an AFB like organism, which we later determined to be 
negative.
Thanks!

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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[Histonet] AFB stained smears

[Glenn Hauck]

The odd time I receive a request to do a Zeihl Neelsen stain for acid fast 
bacteria on a smear for Micro. My question is what kind of control should I be 
using? I have tissue processed with AFB, but I feel this should not be used 
because the smear is not processed the same way as my AFB control block.

Thanks

Glenn Hauck
Charge Technologist
Pathology
Queen Elizabeth II Hospital
Grande Prairie, AB T8V 2E8

780-538-7429 Work Main
780-538-7184 Work Office
glenn.ha...@albertahealthservices.ca



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Re: [Histonet] AFB stained smears

[Patsy Ruegg]

U r right ffpe control would not be ideal but unless u have access to a source 
+ for tb u could make a bunch of smears to keep for controls that may be your 
only option

Sent from my iPhone

 On Nov 7, 2014, at 10:38 AM, Glenn Hauck 
 glenn.ha...@albertahealthservices.ca wrote:
 
 The odd time I receive a request to do a Zeihl Neelsen stain for acid fast 
 bacteria on a smear for Micro. My question is what kind of control should I 
 be using? I have tissue processed with AFB, but I feel this should not be 
 used because the smear is not processed the same way as my AFB control block.
 
 Thanks
 
 Glenn Hauck
 Charge Technologist
 Pathology
 Queen Elizabeth II Hospital
 Grande Prairie, AB T8V 2E8
 
 780-538-7429 Work Main
 780-538-7184 Work Office
 glenn.ha...@albertahealthservices.ca
 
 
 
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 recipient(s), are confidential and may contain privileged information. Any 
 unauthorized review, use, retransmission, or other disclosure is strictly 
 prohibited. If you have received this message in error, please notify the 
 sender immediately, and then delete the original message. Thank you.
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[Histonet] AFB Control Blocks

[Pairan, Kelly]

Good Morning,
We are currently cutting controls from our last few AFB control blocks.  We are 
wondering if anyone has any they can spare?   Please contact me at 
kelly.pai...@nationwidechildrens.orgmailto:kelly.pai...@nationwidechildrens.org.
  We are prepared to pay for the shipping.

Thanks!
Kelly
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[Histonet] AFB Control MANY THANKS!

[Cheryl]

Many thanks to all who responded!  We'll be in good shape in a few days.
 
Appreciate the depth of knowledge with which you are all so generous~~always!
 
Happy Friday!
 
Cheryl

Cheryl Kerry, HT(ASCP) 
Full Staff Inc. 
Staffing the AP Lab by helping one GREAT Tech at a time.  
281.852.9457 Office
800.756.3309 Phone  Fax 
ad...@fullstaff.org 
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[Histonet] AFB Controls

[Cheryl]

Help?

We are about to run out of AFB control slides and haven't had a good loaded 
case in a while.  Is there an easy way to come up with an AFB positive block or 
could someone lend me one to be replaced at a later date? (Go through too many 
to be cost effective to buy)

OR is there something out in the world I can use to make a control?

Please respond to tkngfl...@yahoo.com
 
Cheryl Kerry, HT(ASCP) 
 
281.852.9457 Office
800.756.3309 Phone  Fax 
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RE: [Histonet] AFB Controls

[Wineman, Terra]

Sigma provides AFB control slides if you can't get block.  The item number is 
A2299

Terra Wineman, HTL (ASCP)CM
Research Biologist
636-926-7476 phone
terra.wine...@novusint.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Thursday, October 10, 2013 4:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AFB Controls

Help?

We are about to run out of AFB control slides and haven't had a good loaded 
case in a while.  Is there an easy way to come up with an AFB positive block or 
could someone lend me one to be replaced at a later date? (Go through too many 
to be cost effective to buy)

OR is there something out in the world I can use to make a control?

Please respond to tkngfl...@yahoo.com
 
Cheryl Kerry, HT(ASCP) 
 
281.852.9457 Office
800.756.3309 Phone  Fax 
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Re: [Histonet] AFB Controls

[Hans B Snyder]

I know source medical products has them cheaper than newcomer supply.
 E-mail and ask for Walter.

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com ha...@histologistics.com


On Thu, Oct 10, 2013 at 5:24 PM, Cheryl tkngfl...@yahoo.com wrote:

 Help?

 We are about to run out of AFB control slides and haven't had a good
 loaded case in a while.  Is there an easy way to come up with an AFB
 positive block or could someone lend me one to be replaced at a later date?
 (Go through too many to be cost effective to buy)

 OR is there something out in the world I can use to make a control?

 Please respond to tkngfl...@yahoo.com

 Cheryl Kerry, HT(ASCP)

 281.852.9457 Office
 800.756.3309 Phone  Fax
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Re: [Histonet] AFB Controls

[Lee Peggy Wenk]

Make your own.

Take some fresh lung (slightly edematous is better, if you can get it). Cut 
into 2x2 mm cubes (or largest 3x3 mm cubes).


Contact Microbiology, and have them make a broth with a non-pathogenic AFB 
in a large tube (e.g., plastic centrifuge tube). Put lung cubes in broth. 
Incubate overnight (I found that room temp is usually OK).


Next morning, add 10% NBF. Wait about an hour or so, then dispose of NBF, 
and add fresh 10% NBF.  (The first NBF is diluted by the broth, and by 
allowing the NBF to sit in the broth for a while, it kills the bacteria. 
Adding the 2nd NBF allows the tissue to be fixed in 10% NBF, rather than 
diluted NBF.)


Allow to fix most of the day, put tissue in cassettes, process as usual, 
embed, and you have lots of AFB controls for really cheap. Write up a cost 
containment (how much it cost you to make X number of blocks that you can 
get Y number of control slides from vs. the cost of buying the same number 
of Y slides from a vendor.) Management will love you for your cost 
containment.


Works for gram +, gram -, and fungus (get the correct broth). Will work for 
spirochete too, but our micro lab has only a LARGE non-pathogenic 
spirochete, which is much larger than syphilis. So doing a Steiner stain 
would most likely yield a false-negative. (When the large spirochetes 
control is seen, the little syphilis would not be stained.)


Please realize, these controls may not work for IHC. Better check on the 
genus. They work great for Kinyoun/Ziehl-Neelsen/Fites, Brown and 
Hopps/Brown and Brenn, GMS/PAS, Steiner/Warthin-Starry.


Peggy Wenk, HTL(ASCP)SLS

-Original Message- 
From: Cheryl

Sent: Thursday, October 10, 2013 5:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AFB Controls

Help?

We are about to run out of AFB control slides and haven't had a good loaded 
case in a while.  Is there an easy way to come up with an AFB positive block 
or could someone lend me one to be replaced at a later date? (Go through too 
many to be cost effective to buy)


OR is there something out in the world I can use to make a control?

Please respond to tkngfl...@yahoo.com

Cheryl Kerry, HT(ASCP)

281.852.9457 Office
800.756.3309 Phone  Fax
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[Histonet] AFB stain

[Nancy Schmitt]

We run the AFB on the Dako Artisan - recently it has been coming off with VERY 
intense blue staining.  Tech. support offered a new kit - same problem.  We 
lessened the time and got the same result.  All other stains on the Artisan are 
working.  Thoughts are appreciated!

Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories
Dubuque, IA  52001



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[Histonet] AFB control

[Cynthia Robinson]

All,

Does anyone have AFB control in human tissue they would be willing to share? I 
shared fungal controls awhile back with anyone who emailed methat is, until 
I ran out. I would be willing to share control material if I have something you 
need.

Thanks.


Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com



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RE: [Histonet] AFB control

[Lynette Pavelich]

If you join the Michigan Society of Histotechnologists for $20/year membership, 
you will receive not only the award winning MikroGraf newsletter, voted #1 by 
the NSH, but have access to a supply of control blocks as needed for no extra 
charge!

Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503

ph: 810.262.9948
mobile: 810.444.7966


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Cynthia Robinson 
[robin...@mercyhealth.com]
Sent: Wednesday, November 07, 2012 9:20 AM
To: histonet
Subject: [Histonet] AFB control

All,

Does anyone have AFB control in human tissue they would be willing to share? I 
shared fungal controls awhile back with anyone who emailed methat is, until 
I ran out. I would be willing to share control material if I have something you 
need.

Thanks.


Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com



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[Histonet] AFB by IHC

[Amy Self]

My pathologist wants an AFB stain done by IHC.  Is this stain/procedure even 
doable and if so where?

Thanks in advance for your help,  Amy



Amy Self
Georgetown Hospital System
843-527-7179
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Re: [Histonet] AFB by IHC

[Rene J Buesa]

Amy:
I think you should clarify with your pathologist this request because AFB, as 
you perfectly know, is a procedure in itself. Is it that s/he wants to detect 
acid fast bacteria with an IHC method instead of the Ziehl-Nielsen method?
In that case your only option is to look in the IHC providers catalogs if an 
antibody has been developed for  the different types of Mycobacteria and use it.
Perhaps your pathologist has found out that H. pyloris are now detected in many 
labs using IHC and s/he wants to do the same with Mycobacteria.
You will need to get a clarification about that request.
René J.


--- On Tue, 4/3/12, Amy Self as...@georgetownhospitalsystem.org wrote:


From: Amy Self as...@georgetownhospitalsystem.org
Subject: [Histonet] AFB by IHC
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
Date: Tuesday, April 3, 2012, 11:48 AM


My pathologist wants an AFB stain done by IHC.  Is this stain/procedure even 
doable and if so where?

Thanks in advance for your help,  Amy



Amy Self
Georgetown Hospital System
843-527-7179
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Re: [Histonet] AFB by IHC

[Richard Cartun]

Yes, you can do IHC for mycobacteria; however, there are issues with 
specificity depending on the primary antibody that you use.  I have been using 
a polyclonal antibody from Dako (no longer available) for many years and 
although it is very sensitive for identifying mycobacteria, it does cross-react 
with other bacteria.  I have not found a monoclonal antibody (yet) that works 
on formalin-fixed, paraffin-embedded tissue.  In addition, your IHC assay has 
to be exquisitely clean in order to trust the rare immunoreactivity that may be 
present.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


 Amy Self as...@georgetownhospitalsystem.org 4/3/2012 11:48 AM 
My pathologist wants an AFB stain done by IHC.  Is this stain/procedure even 
doable and if so where?

Thanks in advance for your help,  Amy



Amy Self
Georgetown Hospital System
843-527-7179
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[Histonet] AFB control

[Stephanie Hoyle-Thacker]

Is anyone using a negative control for AFB stains?  If so is there a regulation 
that states negative controls should be run for AFB stains.  I seem to recall 
seeing such a regulation but cannot find it.
 
Thanks for your help!
 
Renee Thacker 
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Re: [Histonet] AFB control

[Rene J Buesa]

All I recall about controls in HC procedures (any of them) is to have a (+) 
control. The only one that requires both (-) and (+) is PAS
René J.

--- On Fri, 2/10/12, Stephanie Hoyle-Thacker srhthac...@hotmail.com wrote:


From: Stephanie Hoyle-Thacker srhthac...@hotmail.com
Subject: [Histonet] AFB control
To: histonet@lists.utsouthwestern.edu
Date: Friday, February 10, 2012, 1:52 PM



Is anyone using a negative control for AFB stains?  If so is there a regulation 
that states negative controls should be run for AFB stains.  I seem to recall 
seeing such a regulation but cannot find it.

Thanks for your help!

Renee Thacker               
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RE: [Histonet] AFB control

[Debra Siena]

It is also a requirement if you are doing work in or for the state of New York. 
 

Debbie Siena
800.442.3573 ext. 229 | www.statlab.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Friday, February 10, 2012 2:49 PM
To: Jennifer MacDonald; Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu; Stephanie Hoyle-Thacker; 
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] AFB control

Recommended by who?
Mike

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Friday, February 10, 2012 2:43 PM
To: Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu; Stephanie Hoyle-Thacker; 
histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] AFB control


It is recommended to run a negative AFB control to rule out false 
positives.  Some sources of water have acid fast organisms, not TB, and if 
the water is used in the water baths or for staining you could pick up 
those organisms.  The recommendation is to cut a section of tissue, known 
NOT to contain AFB organisms, and run it through with the positive control 
and patient.  You would pick it up on the same water bath that you used 
for the patient.




Rene J Buesa rjbu...@yahoo.com 
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/10/2012 11:55 AM

To
histonet@lists.utsouthwestern.edu, Stephanie Hoyle-Thacker 
srhthac...@hotmail.com
cc

Subject
Re: [Histonet] AFB control






All I recall about controls in HC procedures (any of them) is to have a 
(+) control. The only one that requires both (-) and (+) is PAS René J.

--- On Fri, 2/10/12, Stephanie Hoyle-Thacker srhthac...@hotmail.com 
wrote:


From: Stephanie Hoyle-Thacker srhthac...@hotmail.com
Subject: [Histonet] AFB control
To: histonet@lists.utsouthwestern.edu
Date: Friday, February 10, 2012, 1:52 PM



Is anyone using a negative control for AFB stains?  If so is there a 
regulation that states negative controls should be run for AFB stains.  I 
seem to recall seeing such a regulation but cannot find it.

Thanks for your help!

Renee Thacker   
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[Histonet] AFB Controls

[Marcum, Pamela A]

Does anyone know where we can get a good AFB control?  Ours have exhausted and 
we can't find a case here with a positive reading.

Best Regards,

Pamela A Marcum
UAMS
Slot 502
4301 W Markham Street
Little Rock AR 72205
Office: 501-686-7554
Fax: 501-686-7151

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[Histonet] AFB stains, shiny water artifact, in resource limited countries

[alineumann]

Hi, I am volunteering in a 300 bed Kenya hospital for a month (Kijabe), and we 
have a problem with numerous shiny red artifacts, some of which are the shape 
and size of TB (which are very distracting), apparently caused by processing 
with 70% alcohol.  Does anyone know a way to eliminate these while still using 
70% isopropryl, which is apparently the only economically feasible processing 
reagent available here?  Would it help to soak the blocks in 95% before cutting 
(we have small amounts of 95% available).  Thanks very much in advance!  Alice


Alice Neumann MD
Western Wyoming Pathology PC
Pinnacle Pathology PC
9423 West Kentucky Place
Lakewood, CO   80226
Phone:  303-989-3098

24 Hour Cell Phone:  307-413-4092
alineum...@aol.com 
 
 
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RE: [Histonet] AFB

[Weems, Joyce]

I thought Fite's was more specific for leprosy.. whatever that bug is called 
now. j 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder
Sent: Saturday, October 16, 2010 10:49
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AFB

Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast 
bacteria.  The Fite's stain is usually considered specific for mycobacteria.
Cheryl
 
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Re: [Histonet] AFB

[Lee Peggy Wenk]

Hi - Can't find the original question - must have deleted it, so I'll answer 
through Cheryl's, if that's OK with her.


If I remember, the question was something like - are there other 
microorganisms besides TB that stain with Kinyoun (or something like that).


First, any mycobacterium (acid fast bacteria AFB) will stain with 
Ziehl-Neelsen, Kinyoun, Auramine-Rhodamine or Fites (hence anything staining 
with these stains is called AFB positive, meaning staining with these AF 
stains) . So that means Mycobacterium tuberculosis (TB), Mycobacterium avium 
intracellular (found in birds and I've seen it in spleen and bone marrow of 
immune suppressed patients), Mycobacterium paratuberculosis (found in 
intestine of infected cows and goats, causing something similar to Crone's 
disease), and a lot of other Mycobacterium are AFB positive staining with 
these procedures.


Mycobacterium leprae (leprosy) is a very thin walled mycobacterium, so needs 
the Fites stain with the peanut oil (or any other oil, such as mineral oil) 
to coat the leprosy microorganism, so it can withstand decolorization with 
aqueous alcohol. But Fites can also be used to demonstrate any of the 
Mycobacterium.


These AFB stains will also demonstrate the sulfur clubs of actinomyces, and 
the filamentous strands of nocardia are also weakly AFB positive. (Sort of 
bacteria with some fungus characteristics.)


Cryptosporidium found in the intestine (causing severe diarrhea) can also 
stain AFB positive.


And there are bacterium (mycobacterium or others) in tap water that are AFB 
positive, which can adhere to your slides from the flotation bath water, or 
from the tap water.  Usually non-pathogenic AFB, but I don't know their 
names.


And I'm sure there are other less common bacteria that I've forgotten or 
never known about that will stain AFB positive, which are not in the 
Mycobacterium genus.


And let's remember that hair follicles, sperm (heads with lipids), 
lipofucsin (wear and tear pigment of lipids) also stain AFB positive. And 
also Russell bodies (inclusions of antibodies in plasma cells) will stain 
AFB positive. And keratin which is dense can simply require longer 
differentiation, or else it will retain the red color of the dye.


So, yes, Amy, there are other things that stain positive with Kinyoun that 
are not TB/mycobacterium.


Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

--
From: Cheryl Crowder ccrow...@vetmed.lsu.edu
Sent: Saturday, October 16, 2010 10:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AFB


Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast
bacteria.  The Fite's stain is usually considered specific for 
mycobacteria.

Cheryl

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[Histonet] AFB

[Cheryl Crowder]

Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast 
bacteria.  The Fite's stain is usually considered specific for mycobacteria.
Cheryl
 
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[Histonet] AFB stain

[Amy Farnan]

Have any of you know  heard of  AFB will picking up in any other organisms 
beside mycobacterium? 
 
Amy

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[Histonet] afb contamination

[Tench, Bill]

We had a problem with contamination on our AFB stains, and we discovered
that it was the control slide flaking off in the copland jar which was
being used for staining the control and target slide at the same time
(makes sense as a real control').  We identified these contaminants
because they were frequently large clusters (by large I would say 4-8
organisms) which we almost never see in real cases, and fortunately,
they were also not in the same plane of focus (but that can be subtle).
they did create problems.  Our solution was to stain the control
separately from the case.  No more problems. 

 

Bill Tench

Associate Dir. Laboratory Services

Chief, Cytology Services

Palomar Medical Center

555 E. Valley Parkway

Escondido, California  92025

bill.te...@pph.org

Voice: 760- 739-3037

Fax: 760-739-2604

 


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Re: [Histonet] afb contamination

[Jackie M O'Connor]

You can eliminate the crossover contamination by performing the stain 
horizontally on a staining rack.  A lot of labs re-use the carbol fuchsin, 
which can have lots of extraneous AFB floating around, waiting patiently 
to attach itself to an unsuspecting patient slide.  Using the staining 
rack, you don't use a lot of reagent, and you never cross-contaminate.




From:
Tench, Bill bill.te...@pph.org
To:
histonet@lists.utsouthwestern.edu
Date:
05/21/2010 02:34 PM
Subject:
[Histonet] afb contamination
Sent by:
histonet-boun...@lists.utsouthwestern.edu



We had a problem with contamination on our AFB stains, and we discovered
that it was the control slide flaking off in the copland jar which was
being used for staining the control and target slide at the same time
(makes sense as a real control').  We identified these contaminants
because they were frequently large clusters (by large I would say 4-8
organisms) which we almost never see in real cases, and fortunately,
they were also not in the same plane of focus (but that can be subtle).
they did create problems.  Our solution was to stain the control
separately from the case.  No more problems. 

 

Bill Tench

Associate Dir. Laboratory Services

Chief, Cytology Services

Palomar Medical Center

555 E. Valley Parkway

Escondido, California  92025

bill.te...@pph.org

Voice: 760- 739-3037

Fax: 760-739-2604

 


mail2.pph.org made the following annotations
-
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entity to which it is addressed, and may contain information that is 
privileged, confidential, or otherwise protected from disclosure. 
Dissemination, distribution, or copying of this e-mail or the information 
herein by anyone other than the intended recipient is prohibited.  If you 
have received this e-mail in error, please notify the sender by reply 
e-mail, and destroy the original message and all copies.
-

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RE: [Histonet] AFB Control Blocks

[WILLIAM DESALVO]

Concerning Control Tissue Blocks:

The NSH provides and Share and Exchange Service for members and non-members 
alike. Anytime you have a need for a particular type of control, please contact 
the NSH office (nsh.org) and we will try and meet your needs. The program is 
only intended for individual lab use and not commercial use (selling control 
slides). If you have any questions you can direct them to me and I will assist 
in any way I can.

William DeSalvo, B.S., HTL(ASCP)

Chair, NSH Quality Control Committee




 
 From: jackd...@msn.com
 To: histonet@lists.utsouthwestern.edu
 Date: Thu, 22 Oct 2009 15:31:10 -0400
 Subject: [Histonet] AFB Control Blocks
 
 
 Can anyone help me in getting some good AFB control blocks? I have 
 Pneumocystis Controls‏ Blocks for trade. Help! 
 _
 Windows 7: It helps you do more. Explore Windows 7.
 http://www.microsoft.com/Windows/windows-7/default.aspx?ocid=PID24727::T:WLMTAGL:ON:WL:en-US:WWL_WIN_evergreen3:102009
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[Histonet] AFB Control Blocks

[Jack Dodo]

Can anyone help me in getting some good AFB control blocks? I have Pneumocystis 
Controls‏ Blocks for trade. Help! 
_
Windows 7: It helps you do more. Explore Windows 7.
http://www.microsoft.com/Windows/windows-7/default.aspx?ocid=PID24727::T:WLMTAGL:ON:WL:en-US:WWL_WIN_evergreen3:102009
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[Histonet] AFB control slides

[Vickroy, Jim]

I have struck out with a vendor for AFB control slides.   Anybody have a 
supplier of AFB control slides?

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



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Fw: Re: AW: [Histonet] AFB Recycling Formalin for Fresh Tissue

[Akemi Allison-Tacha]

Hello,
I responded to Gudrun personally, but I thought that the information I am 
supplying him below might be a good educational source for other histologists 
who are having similar scenario's for AFB contamination.  

I would also like to say that I have an extremely eager histology team that 
have expressed an interest in learning theory and practice.  Most of my team 
are OJT.  They have had several pathologists approach them with these concerns 
regarding AFB, and because of their knowledge, or lack of it, couldn't fix the 
problem.  We are starting with the basics and I am giving in-services along the 
way.

Hi  Gudrun,

They are shipping the recycled formalin directly to the outside hospital 
surgery to put their fresh tissues into.

As far as the AFB, we have had intermitant (+) AFB on the edges of tissues, 
that has been extremely questionable.  We currently are not cutting a negative 
(-)  positive(+) control on the same water bath as the test sample. That will 
be remedied beginning Monday. The water here is also in question.  I will be 
sending it out for microbacteria analysis.

We are going through a thorough housekeeping and in-service process.  We are 
starting with the tissue processors, which are currently only changed weekly 
(not rotated daily), embedding centers, which have to my knowledge, never been 
drained, and the hopper cleaned-out.  The water baths are not scrubbed out with 
soap and HOT water daily and
 covered at night to prevent contamination.  The water bath is not skimmed of 
excess tissue debris, each and every time another specimen is placed on it.  
The Carbol Fuchsin is not being filtered prior to use.  The working solution is 
currently being poured back into the stock bottle.  Need I go on

Akemi Allison-Tacha BS, HT (ASCP) HTL
Histology Manager
Associated Pathology Medical Group Laboratories
105A Cooper Ct. Los Gatos, CA 95032
Cell: 425.941.4287 
W: E-Mail: aallison-ta...@apmglab.com
P: E-Mail: akemiat3...@yahoo.com



--- On Thu, 5/28/09, Gudrun Lang gu.l...@gmx.at wrote:

From: Gudrun Lang gu.l...@gmx.at
Subject: AW: [Histonet] Recycling Formalin for Fresh Tissue
To: akemiat3...@yahoo.com
Cc:
 histonet@lists.utsouthwestern.edu
Date: Thursday, May 28, 2009, 10:34 AM

Just for interest. Your lab takes the recycled formalin to make 4% neutral
buffered formalin (NBF)? Or do they ship the recycled formalin directly in
bottles to the surgery?
AFB = acid fast bacili?

Do you have concerns, that they produce false-positive AFB stains? I cannot
imagine, that a (hopefully) small number of dead bacili on the surface of
tissue will pretend this. An other question: Isn't there a filter in the
recycler to prevent such contamination?
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von
akemiat3...@yahoo.com
Gesendet: Donnerstag, 28. Mai 2009 18:14
An: Histonet
Betreff: [Histonet] Recycling Formalin for Fresh Tissue

Good Morning Histo Land,

I am asking all you out there to give me your input on recycIing formalin. 
I realize this has been discussed in the not too distant past, but this may
be a little different situation.  I realize some labs are recycling formalin
to put on their tissue processors, but this is a totally different scenario.

I have been a manager at my lab for only a month.  Much to my surprise, I
just found out yesterday that our lab is recycling formalin and shipping it
out to one of our clients to put their FRESH SPECIMENS into. 
 

I just had a lengthy conversation with Robert Lott the day before yesterday,
regarding pre-analytical protocol's effecting the test results of AFB.  By
the way, we were in full agreement.  This formalin issue was unknown to me
then.  I realize that the pre-analytical, pre-analytical, pre-analytical,
process effects the final out come.  I need to present my case to our
Director of Pathology why this is compromising patient care.

Any and all responses are gladly welcome.

Thanks,
Akemi

Akemi Allison-Tacha BS, HT (ASCP) HTL
Histology Manager
Associated Pathology Medical Group Laboratories
105A Cooper Ct. Los Gatos, CA 95032
Cell: 425.941.4287 
W: E-Mail: aallison-ta...@apmglab.com
P: E-Mail: akemiat3...@yahoo.com

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Re: Fw: Re: AW: [Histonet] AFB Recycling Formalin for Fresh Tissue

[Freida Carson]

Wang reported on the contamination of tissue sections with AFB by the use of 
fluorescence microscopy, and if I remember correctly, when he tested the water 
in water fountains, he found something like 33% contained AFB.  Non-pathogenic, 
but we can't tell that on our stains. The paper is in Am J Clin Pathol 51:71, 
1969.  We (Carson, Kingsley, Haberman, and Race) also reported the 
contamination in the 1964 issue of the same journal.  We stopped using tap 
water for our water baths and began using millipore filtered water that had 
already been through a deionizing column and charcoal filter.  We always cut a 
negative control from the same days workload as the patient case - uterus in 
our case.  You don't need a positive control cut the same day, just a positive 
control with only a medium number of organisms.  We also did not use any tap 
water in the deparaffinization prior to the carbol fuchsin.  AFB organisms have 
also been reported growing in 40
 gal formalin tanks believe it or not and in the old Fisher Paraffin wafers.
 
We centrifuged tap water in 50-ml tubes, poured off the supernatent, and 
refilled and recentrifuged until we could see some sediment in the bottom.  We 
took that to Microbioloby and had it cultured to definited prove the presence 
of AFB in the tap water.
I would recommend this approach to anyone suspecting tissue contamination. 
 
Freida Carson
--- On Thu, 5/28/09, Akemi Allison-Tacha akemiat3...@yahoo.com wrote:


From: Akemi Allison-Tacha akemiat3...@yahoo.com
Subject: Fw: Re: AW: [Histonet] AFB  Recycling Formalin for Fresh Tissue
To: Histonet Histonet@lists.utsouthwestern.edu
Date: Thursday, May 28, 2009, 1:21 PM


Hello,
I responded to Gudrun personally, but I thought that the information I am 
supplying him below might be a good educational source for other histologists 
who are having similar scenario's for AFB contamination.  

I would also like to say that I have an extremely eager histology team that 
have expressed an interest in learning theory and practice.  Most of my team 
are OJT.  They have had several pathologists approach them with these concerns 
regarding AFB, and because of their knowledge, or lack of it, couldn't fix the 
problem.  We are starting with the basics and I am giving in-services along the 
way.

Hi  Gudrun,

They are shipping the recycled formalin directly to the outside hospital 
surgery to put their fresh tissues into.

As far as the AFB, we have had intermitant (+) AFB on the edges of tissues, 
that has been extremely questionable.  We currently are not cutting a negative 
(-)  positive(+) control on the same water bath as the test sample. That will 
be remedied beginning Monday. The water here is also in question.  I will be 
sending it out for microbacteria analysis.

We are going through a thorough housekeeping and in-service process.  We are 
starting with the tissue processors, which are currently only changed weekly 
(not rotated daily), embedding centers, which have to my knowledge, never been 
drained, and the hopper cleaned-out.  The water baths are not scrubbed out with 
soap and HOT water daily and
covered at night to prevent contamination.  The water bath is not skimmed of 
excess tissue debris, each and every time another specimen is placed on it.  
The Carbol Fuchsin is not being filtered prior to use.  The working solution is 
currently being poured back into the stock bottle.  Need I go on

Akemi Allison-Tacha BS, HT (ASCP) HTL
Histology Manager
Associated Pathology Medical Group Laboratories
105A Cooper Ct. Los Gatos, CA 95032
Cell: 425.941.4287 
W: E-Mail: aallison-ta...@apmglab.com
P: E-Mail: akemiat3...@yahoo.com



--- On Thu, 5/28/09, Gudrun Lang gu.l...@gmx.at wrote:

From: Gudrun Lang gu.l...@gmx.at
Subject: AW: [Histonet] Recycling Formalin for Fresh Tissue
To: akemiat3...@yahoo.com
Cc:
histonet@lists.utsouthwestern.edu
Date: Thursday, May 28, 2009, 10:34 AM

Just for interest. Your lab takes the recycled formalin to make 4% neutral
buffered formalin (NBF)? Or do they ship the recycled formalin directly in
bottles to the surgery?
AFB = acid fast bacili?

Do you have concerns, that they produce false-positive AFB stains? I cannot
imagine, that a (hopefully) small number of dead bacili on the surface of
tissue will pretend this. An other question: Isn't there a filter in the
recycler to prevent such contamination?
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von
akemiat3...@yahoo.com
Gesendet: Donnerstag, 28. Mai 2009 18:14
An: Histonet
Betreff: [Histonet] Recycling Formalin for Fresh Tissue

Good Morning Histo Land,

I am asking all you out there to give me your input on recycIing formalin. 
I realize this has been discussed in the not too distant past, but this may
be a little different situation.  I realize some labs are recycling formalin
to put on their tissue processors

Re: Fw: Re: AW: [Histonet] AFB Recycling Formalin for Fresh Tissue

[Akemi Allison-Tacha]

Thanks Freida,

What better source to validate the steps I am taking to eliminate the problem 
of AFB Contaminate.  

I would also like to mention in my haste to compose my e-mail, I may have 
stated something incorrectly.  I did not mean that a positive (+) control be 
cut at the same time as the test sample and negative (-) control.  I usually 
place the test tissue on the same pre-cut positive (+) control slide.

Thanks Rene,

For your input on recycling formalin.  This is good ammunition for my defense 
in stopping the use of recycling formalin.  I still would welcome any and all 
replies.

Akemi


Akemi Allison-Tacha BS, HT (ASCP) HTL
Histology Manager
Associated Pathology Medical Group Laboratories
105A Cooper Ct. Los Gatos, CA 95032
Cell: 425.941.4287 
W: E-Mail: aallison-ta...@apmglab.com
P: E-Mail: akemiat3...@yahoo.com



--- On Thu, 5/28/09, Freida Carson frei...@sbcglobal.net wrote:

From: Freida Carson frei...@sbcglobal.net
Subject: Re: Fw: Re: AW: [Histonet] AFB  Recycling Formalin for Fresh Tissue
To: Histonet Histonet@lists.utsouthwestern.edu, Akemi Allison-Tacha 
akemiat3...@yahoo.com
Date: Thursday, May 28, 2009, 12:09 PM

Wang reported on the contamination of tissue sections with AFB by the use of 
fluorescence microscopy, and if I remember correctly, when he tested the water 
in water fountains, he found something like 33% contained AFB.  Non-pathogenic, 
but we can't tell that on our stains. The paper is in Am J Clin Pathol 51:71, 
1969.  We (Carson, Kingsley, Haberman, and Race) also reported the 
contamination in the 1964 issue of the same journal.  We stopped using tap 
water for our water baths and began using millipore filtered water that had 
already been through a deionizing column and charcoal filter.  We always cut a 
negative control from the same days workload as the patient case - uterus in 
our case.  You don't need a positive control cut the same day, just a positive 
control with only a medium number of organisms.  We
 also did not use any tap water in the deparaffinization prior to the carbol 
fuchsin.  AFB organisms have also been reported growing in 40 gal formalin 
tanks believe it or not and in the old Fisher Paraffin wafers.
 
We centrifuged tap water in 50-ml tubes, poured off the supernatent, and 
refilled and recentrifuged until we could see some sediment in the bottom.  We 
took that to Microbioloby and had it cultured to definited prove the presence 
of AFB in the tap water.
I would recommend this approach to anyone suspecting tissue contamination. 
 
Freida Carson
--- On Thu, 5/28/09, Akemi Allison-Tacha akemiat3...@yahoo.com wrote:


From: Akemi Allison-Tacha akemiat3...@yahoo.com
Subject: Fw: Re: AW: [Histonet] AFB  Recycling Formalin for Fresh Tissue
To: Histonet Histonet@lists.utsouthwestern.edu
Date: Thursday, May 28, 2009, 1:21 PM


Hello,
I responded to Gudrun personally, but I thought that the information I am 
supplying him below might be a good educational source for other histologists 
who are having similar scenario's for AFB contamination.  

I would also like to say that I have an extremely eager histology team that 
have expressed an interest in learning theory and practice.  Most of my team 
are OJT.  They have had several pathologists approach them with these concerns 
regarding AFB, and because of their knowledge, or lack of it, couldn't fix the 
problem.  We are starting with the basics and I am giving in-services along the 
way.

Hi  Gudrun,

They are shipping the recycled formalin directly to the outside hospital 
surgery to put their fresh tissues into.

As far as the AFB, we have had intermitant (+) AFB on the edges of tissues, 
that has been extremely questionable.  We currently are not
 cutting a negative (-)  positive(+) control on the same water bath as the 
test sample. That will be remedied beginning Monday. The water here is also in 
question.  I will be sending it out for microbacteria analysis.

We are going through a thorough housekeeping and in-service process.  We are 
starting with the tissue processors, which are currently only changed weekly 
(not rotated daily), embedding centers, which have to my knowledge, never been 
drained, and the hopper cleaned-out.  The water baths are not scrubbed out with 
soap and HOT water daily and
covered at night to prevent contamination.  The water bath is not skimmed of 
excess tissue debris, each and every time another specimen is placed on it.  
The Carbol Fuchsin is not being filtered prior to use.  The working solution is 
currently being poured back into the stock bottle.  Need I go on

Akemi Allison-Tacha BS, HT (ASCP)
 HTL
Histology Manager
Associated Pathology Medical Group Laboratories
105A Cooper Ct. Los Gatos, CA 95032
Cell: 425.941.4287 
W: E-Mail: aallison-ta...@apmglab.com
P: E-Mail: akemiat3...@yahoo.com



--- On Thu, 5/28/09, Gudrun Lang gu.l...@gmx.at wrote:

From: Gudrun Lang gu.l...@gmx.at
Subject: AW: [Histonet] Recycling Formalin for Fresh

[Histonet] AFB Fites of cytospins

[Rene J Buesa]

Carrie:
The cytospin will not need dewaxing, as you point out, but I do not foresee any 
problem when compared with the control. You have to realize that dewaxing the 
control is intended only to expose the tissue section to the reagents.
As to the peanut oil if you are trying to stain for common Mycobacterium it 
is not necessary because their cover is quite strong. If you are trying to 
detect M. leprae it is when you use the mixture of peanut oil (or any other 
vegetable oil for that matter) mixed with xylene. The rationale is that the oil 
will protect the delicate cover of the organism.
But again, not even for M. leprae you will need the mixture of oil and xylene 
if you are using a cytospin, because they do not require deparafinization 
(dewaxing).
René J.

--- On Mon, 5/18/09, Carrie Disbrow dis...@shands.ufl.edu wrote:


From: Carrie Disbrow dis...@shands.ufl.edu
Subject: [Histonet] AFB Fites of cytospins
To: histonet@lists.utsouthwestern.edu
Date: Monday, May 18, 2009, 6:12 PM


Hello Histonet.
Has anyone had experience and good results doing AFB Fites on cytospin slides 
from BAL's?
Do you use the peanut oil/xylene (PO/Z) solution or just 100% peanut oil? Or 
does the cytospin slide need any peanut oil?
After the deparaffinization step, if it can be called that, we are going to 
stain using a Dako Artisan special stainer. One problem I foresee is that the 
control will be de-arffinized in the traditional PO/Z while the patient slide 
may not.
Also, I was told that Cytolyte contains alcohol and asked that the slides not 
be prepared using Cytolyte.
Thanks for your comments. Please respond back to the histonet if possible and 
not my email address. 
Carrie
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[Histonet] AFB Fites of cytospins

[Carrie Disbrow]

Hello Histonet.
Has anyone had experience and good results doing AFB Fites on cytospin slides 
from BAL's?
Do you use the peanut oil/xylene (PO/Z) solution or just 100% peanut oil? Or 
does the cytospin slide need any peanut oil?
After the deparaffinization step, if it can be called that, we are going to 
stain using a Dako Artisan special stainer. One problem I foresee is that the 
control will be de-arffinized in the traditional PO/Z while the patient slide 
may not.
Also, I was told that Cytolyte contains alcohol and asked that the slides not 
be prepared using Cytolyte.
Thanks for your comments. Please respond back to the histonet if possible and 
not my email address. 
Carrie
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[Histonet] AFB Control

[Lisa S. Black]

We are in need of a good AFB control.  Is there anyone willing to share or 
barter.

Thanks in advance.

Lisa Black, BS, HT(ASCP)
Histology Manager
Carilion Consolidated Lab
933 S Jefferson Street
Roanoke, VA 24016
lbl...@carilion.com 
540-985-4082


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