Re: [Histonet] PAX-5

[Tony Henwood (SCHN) via Histonet]

Hi Beth,
We have found the same thing.

You get a moderate staining (half of what you see with most B cell lymphomas) 
with clone 1EW (as supplied with the Leica Bond rtu).
You will get similar results with clone 24 (38%:  Adams, et al (2009). 
Clinical, phenotypic and genetic similarities and disparities between 
post-transplant and classical Hodgkin lymphomas with respect to therapeutic 
targets. Expert opinion on therapeutic targets, 13(10), 1137-1145.)

We have found that using the Rabbit monoclonal antibody to Pax-5 (Clone SP34), 
it seems that, though staining a similar proportion of B cell lymphomas, 
staining is absent from the Reed-Sternberg cells of Hodgin lymphoma.

Our assessment is in its early stages so with further cases, it might be 
clearer.

It seems that not all Pax-5 antibodies react the same

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: Linda Margraf via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Saturday, 22 July 2017 7:00 AM
To: histonet@lists.utsouthwestern.edu
Cc: 'one...@wvumedicine.org'
Subject: [Histonet] FW: PAX-5

Here is a message I am posting for Beth….


From: "O'neil, Beth" >
Date: July 21, 2017 at 2:31:24 PM CDT
To: 
"histonet-ow...@lists.utsouthwestern.edu"
 
>
Subject: PAX-5
My pathologists are not happy with PAX-5 on the Ventana Ultra; it will 
occasionally show cytoplasmic staining of the Reed-Sternberg cells (supposed to 
be a nuclear stain).  We use Ventana’s clone SP34. I have been trying to 
optimize with the Optiview kit but we are getting so much background staining 
and only sporadic staining of the R-S cells.  I tried a different clone, Cell 
Marque EP156 which was really clean but would not stain the R-S cells at all, 
no matter what we did.   My Ventana technical specialist is stumped as to why 
we’re having so much trouble.  Does anyone else have the same problem or have 
any suggestions?

Beth Ann O’Neil, MT(ASCP)SC, HTL, QIHC
Histology Supervisor, Technical Specialist
Lab:  304 – 293 – 6014
Office:  304 – 293 – 7629
 one...@wvumedicine.org





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[Histonet] PAX-5 staining problems

[Jeffrey Robinson via Histonet]

Good Morning to all:  I am having staining problems with my PAX-5 predilute 
from CellMarque.  I was wondering if anyone else has experienced a dramatic 
dropoff in staining signal with this antibody after a few months.  I have now 
had this happen to me twice in the last 3 months.  It stains very nicely when 
the vial is first opened and seems to hold staining intensity for about 3 
months and then drops off dramatically even though the expiration date is 2 
years out.  I am using it on the Leica Bond.  I have been in contact with 
CellMarque about this problem but they tell me they have not had any complaints 
from any other labs.  I use many antibodies from CellMarque and have only seen 
this problem occur with one other antibody (ALK-1) about a year ago.  If you 
are experiencing this problem in your lab please contact Liz Beitman at 
CellMarque (lbeit...@cellmarque.com) so that 
they can have all available information to further research this problem.  
Thank 
 you!
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.


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[Histonet] PAX-5 - B-cell specific activator protein (BSAP)

[Taylor, Jean]

Hi Everyone,

I'm trying to find out what company and clone most labs are using for PAX-5 
(BSAP).

Thanks!

Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, August 18, 2010 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 81, Issue 23

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Today's Topics:

   1. RE: CD34 positive control (McMahon, Loralee A)
   2. Ventana Renaissance Containers (ricky hachy)
   3. CD34 Control (Silverman, Jeffrey)


--

Message: 1
Date: Wed, 18 Aug 2010 12:17:39 -0400
From: McMahon, Loralee A loralee_mcma...@urmc.rochester.edu
Subject: RE: [Histonet] CD34 positive control
To: Joel Reichensperger jreichensper...@siumed.edu, Histonet
histonet@lists.utsouthwestern.edu
Message-ID:

c27aa2a01cef31469813089e226f582e02d5a7c...@urmcms7.urmc-sh.rochester.edu

Content-Type: text/plain; charset=us-ascii

Just about any tissue should work.   It stains for Hematopoietic 
stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic 
fibroblasts
We use a sausage style control here with about 30 different types of tissue.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger 
[jreichensper...@siumed.edu]
Sent: Wednesday, August 18, 2010 12:05 PM
To: Histonet
Subject: [Histonet] CD34 positive control

  I have a doctor who wants to stain some tissue with cd34. I need to
know if anyone can recommend a good positive control tissue for this
antibody. The staining will be done in paraffin embedded sections.
Thanks in advance.

Joel

--
Joel Reichensperger
Researcher II
Southern Illinois University
Plastic Surgery Institute
jreichensper...@siumed.edu
217-545-7309 (Office)
217-545-1824 (Fax)


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Message: 2
Date: Wed, 18 Aug 2010 16:28:39 +
From: ricky hachy elc...@hotmail.com
Subject: [Histonet] Ventana Renaissance Containers
To: Histonet histonet@lists.utsouthwestern.edu
Message-ID: snt114-w54ab5f08e7e0cfc97193edcf...@phx.gbl
Content-Type: text/plain; charset=iso-8859-1


Hello ,

I need 4 good plastic containers, where you fill with xilene,alcool... for 
the VENTANA RENAISSANCE .

Anyone could sell me ?

Regards

Ricky

--

Message: 3
Date: Wed, 18 Aug 2010 12:30:58 -0400
From: Silverman, Jeffrey jsilver...@nshs.edu
Subject: [Histonet] CD34 Control
To: 'jreichensper...@siumed.edu' jreichensper...@siumed.edu
Cc: 'histonet@lists.utsouthwestern.edu'
histonet@lists.utsouthwestern.edu
Message-ID:
83f4d81747a7094dafe3ae87151ecb941ce4144...@sykechxvs01.nslijhs.net
Content-Type: text/plain; charset=us-ascii

Joel,
A section of skin will be a fine CD34 control. All collagenous connective 
tissue, including the dermis,  are rich in CD34+ dendritic interstitial 
fibroblasts as well as CD34 positive endothelium in the resident vessels. 
Actually, in my lab we use a section of fallopian tube from tubal ligation, 
they are loaded with the fibroblasts and vessels.

Jeff Silverman



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End of Histonet Digest, Vol 81, Issue 23


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RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP)

[Sebree Linda A]

Hi Jean,

We use Cell Marque's (via VMS) clone 24.  We have to throw the entire
arsenal at our disposal at these stains but they're acceptable.

Good luck Jean; any questions, you know where we live! 

Linda


Linda A. Sebree
University of Wisconsin Hospital  Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor,
Jean
Sent: Thursday, August 19, 2010 9:45 AM
To: 'histonet@lists.utsouthwestern.edu'; 'ih...@googlegroups.com'
Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP)

Hi Everyone,

I'm trying to find out what company and clone most labs are using for
PAX-5 (BSAP).

Thanks!

Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, August 18, 2010 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 81, Issue 23

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
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When replying, please edit your Subject line so it is more specific than
Re: Contents of Histonet digest...


Today's Topics:

   1. RE: CD34 positive control (McMahon, Loralee A)
   2. Ventana Renaissance Containers (ricky hachy)
   3. CD34 Control (Silverman, Jeffrey)


--

Message: 1
Date: Wed, 18 Aug 2010 12:17:39 -0400
From: McMahon, Loralee A loralee_mcma...@urmc.rochester.edu
Subject: RE: [Histonet] CD34 positive control
To: Joel Reichensperger jreichensper...@siumed.edu, Histonet
histonet@lists.utsouthwestern.edu
Message-ID:
 
c27aa2a01cef31469813089e226f582e02d5a7c...@urmcms7.urmc-sh.rochester.ed
u

Content-Type: text/plain; charset=us-ascii

Just about any tissue should work.   It stains for Hematopoietic
stem/progenitor cells, bone marrow stromal cells, endothelial cells,
embryonic fibroblasts
We use a sausage style control here with about 30 different types of
tissue.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joel
Reichensperger [jreichensper...@siumed.edu]
Sent: Wednesday, August 18, 2010 12:05 PM
To: Histonet
Subject: [Histonet] CD34 positive control

  I have a doctor who wants to stain some tissue with cd34. I need to
know if anyone can recommend a good positive control tissue for this
antibody. The staining will be done in paraffin embedded sections.
Thanks in advance.

Joel

--
Joel Reichensperger
Researcher II
Southern Illinois University
Plastic Surgery Institute
jreichensper...@siumed.edu
217-545-7309 (Office)
217-545-1824 (Fax)


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Message: 2
Date: Wed, 18 Aug 2010 16:28:39 +
From: ricky hachy elc...@hotmail.com
Subject: [Histonet] Ventana Renaissance Containers
To: Histonet histonet@lists.utsouthwestern.edu
Message-ID: snt114-w54ab5f08e7e0cfc97193edcf...@phx.gbl
Content-Type: text/plain; charset=iso-8859-1


Hello ,

I need 4 good plastic containers, where you fill with
xilene,alcool... for the VENTANA RENAISSANCE .

Anyone could sell me ?

Regards

Ricky

--

Message: 3
Date: Wed, 18 Aug 2010 12:30:58 -0400
From: Silverman, Jeffrey jsilver...@nshs.edu
Subject: [Histonet] CD34 Control
To: 'jreichensper...@siumed.edu' jreichensper...@siumed.edu
Cc: 'histonet@lists.utsouthwestern.edu'
histonet@lists.utsouthwestern.edu
Message-ID:
 
83f4d81747a7094dafe3ae87151ecb941ce4144...@sykechxvs01.nslijhs.net
Content-Type: text/plain; charset=us-ascii

Joel,
A section of skin will be a fine CD34 control. All collagenous
connective tissue, including the dermis,  are rich in CD34+ dendritic
interstitial fibroblasts as well as CD34 positive endothelium in the
resident vessels. Actually, in my lab we use a section of fallopian tube
from tubal ligation, they are loaded with the fibroblasts and vessels.

Jeff Silverman



--

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[IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP)

[Taylor, Jean]

   Dear Sir/Madam,
   Your  email was unable reach the intended person that you were sending
   it to.
   For  more  information  on  our business please click on the following
   link:
   [1]Click here for our website
   We look forward to your continued business in the future.
   Regards,
   Webmaster

References

   1. http://www.downwind.com.au/avdir/rd.php?id=7564
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