RE: [Histonet] Negative controls
We still run negative controls. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Monday, April 28, 2014 4:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. I apologize, I know this question has been asked before. I'm trying to satisfy these requirements in one procedure. Thank you all for your assistance!! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative controls
I just wanted to clarify there are two types of negative controls, one is a negative reagent control and one is a tissue that is known negative. A lot of time, we tend to focus on the negative reagent control and forget about the negative tissue control. I know that a lot of smart folks out there on histonet will be able to give you some direction as far as how to write a policy to cover both but just wanted to clarify that the two types of negative controls are different and the negative reagent control may be abandoned if you are running a polymer detection kit but the known negative tissue controls are still required. Thanks Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t: 800.442.3573 ext. 229 | f: 972.436.1369 dsi...@statlab.com | www.statlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Monday, April 28, 2014 4:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. I apologize, I know this question has been asked before. I'm trying to satisfy these requirements in one procedure. Thank you all for your assistance!! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative controls
This is what I put in my procedure shortly after Richard sent his email. There wasn't a problem at inspection. Following his announcement at NSH/ISH Forum in Windsor, CT, Dr. Richard Carten sent an email to Histonet on July 17, 2102, regarding CAP guidelines for negative controls. The checklist will be changed to reflect that negative controls will no longer be required for polymer based procedures. The new wording contains the following statement: “Immunohistochemical tests using polymer-based systems (biotin-free) are sufficiently free of background reactivity to obviate the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director”. Dr. Stargel has approved the discontinuation of negative controls as of July 20, 2012. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Monday, April 28, 2014 5:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. I apologize, I know this question has been asked before. I'm trying to satisfy these requirements in one procedure. Thank you all for your assistance!! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Negative Controls in IHC
Negative controls for IHC has been discussed before many times but it keeps pupping up every noew and then. This is my take on it: 1- if CAP requires them, so then do it. You do not want to have a negative issue in your inspection 2- it is always good to have them for quality control of your procedure and to prevent any legal issues down the road 3- if I have a case requiring several antibodies, I only run a negative control for the tissue series (the block in question) but not for every antibody but if in that antibodies series there are different detection systems, I run a negative control for each René J. From: Ann Specian thisis...@aol.com To: histonet@lists.utsouthwestern.edu Sent: Saturday, September 29, 2012 1:56 PM Subject: [Histonet] Negative Controls in IHC I have a question in regard to eliminating the use of negative controls when using a non-avidin-biotin detection system. Do you not feel that negative controls may still need to be run in tissues which are likely to be pigmented such as lymph node, skin and liver? We were thinking to eliminate the use of negatives for other tissues (cervical, GI, etc.) only.What is the general consensus? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative Controls in IHC
We have eliminated them all per the CAP guidelines. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Saturday, September 29, 2012 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Controls in IHC I have a question in regard to eliminating the use of negative controls when using a non-avidin-biotin detection system. Do you not feel that negative controls may still need to be run in tissues which are likely to be pigmented such as lymph node, skin and liver? We were thinking to eliminate the use of negatives for other tissues (cervical, GI, etc.) only.What is the general consensus? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
Yes you can, the cap standards just came out Tuesday with the revised part stating you do not have to run a negative reagent control using polymer based detection. Only if you are still using biotin based detection kits do you need to run the neg. control Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vpe...@pathreflab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, August 01, 2012 4:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls just wondering if we can start leaving the negative controls off of our immuno stains now as long as our procedure says we can stain without them? we use ventanas multimer detection systems. thanks so much, anita dudley providence hospital mobile, alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
You may be able to omit them, but in our case they are still a detection that is part of our volume agreement. I'm not sure if everyone else has a similar agreement, but omitting the negative controls may affect your ability to meet a volume commitment. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Vanessa Perez [mailto:vpe...@pathreflab.com] Sent: Thursday, August 02, 2012 8:35 AM To: anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] negative controls Yes you can, the cap standards just came out Tuesday with the revised part stating you do not have to run a negative reagent control using polymer based detection. Only if you are still using biotin based detection kits do you need to run the neg. control Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vpe...@pathreflab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, August 01, 2012 4:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls just wondering if we can start leaving the negative controls off of our immuno stains now as long as our procedure says we can stain without them? we use ventanas multimer detection systems. thanks so much, anita dudley providence hospital mobile, alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
I am hoping that the vendors will recognize this when the make these agreements. Hopefully not doubling our prices! Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdess...@commonwealthhealth.net] Sent: Thursday, August 02, 2012 10:58 AM To: Vanessa Perez; anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] negative controls You may be able to omit them, but in our case they are still a detection that is part of our volume agreement. I'm not sure if everyone else has a similar agreement, but omitting the negative controls may affect your ability to meet a volume commitment. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Vanessa Perez [mailto:vpe...@pathreflab.com] Sent: Thursday, August 02, 2012 8:35 AM To: anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] negative controls Yes you can, the cap standards just came out Tuesday with the revised part stating you do not have to run a negative reagent control using polymer based detection. Only if you are still using biotin based detection kits do you need to run the neg. control Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vpe...@pathreflab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, August 01, 2012 4:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls just wondering if we can start leaving the negative controls off of our immuno stains now as long as our procedure says we can stain without them? we use ventanas multimer detection systems. thanks so much, anita dudley providence hospital mobile, alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
I asked my VMS rep that question and she forwarded it to the higher ups as they had not heard about this yet. Waiting for an answer but even if VMS says we can omit them, our negative aren't all that clean always so don't know if we will or not. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, August 01, 2012 4:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls just wondering if we can start leaving the negative controls off of our immuno stains now as long as our procedure says we can stain without them? we use ventanas multimer detection systems. thanks so much, anita dudley providence hospital mobile, alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative controls and CAP
Hi Chris, CAP actually has a specific checklist question on Negative Controls. The question is ANP.22570 and if I remember correctly it is quite extensive. Most of the CAP questions are about 7 to 10 lines. The Negative control question is more than a page long. Get your hands a copy of the most current checklist for anatomic pathology - That will include Immunohistochemistry. By the way, the rule is that a negative control must be included with each run and there is much, much more. ANP.22570 Good Luck, Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christopher Jacobs Sent: Friday, July 13, 2012 9:31 PM To: histonet@lists.utsouthwestern.edu Cc: cjac...@clinpath.com Subject: [Histonet] Negative controls and CAP Histonetters, I have been made the IHC lead in a fairly large laboratory that is seeking to become CAP accredited. I am definitely a newbie when it comes to CAP. A question was brought up today about how we do our negative controls. Specifically, should we run another negative control with any IHCs that need to be repeated or if we should run another negative control if at a later date a pathologist orders additional IHCs on a case. With our current protocol, we run one negative control per detection kit used, per case. Consequently, most cases end up with just one negative control slide. We do NOT run another negative control if we have to repeat one of a group of immunos or if a pathologist orders additional stains at a later point. I am wondering if this is good practice, and acceptable with CAP. I would love to find some literature that could help me make a case either way on this. Actually, any literature or pointers regarding IHCs and becoming CAP accredited will probably help save what little hair I have left. Thank you, Chris Jacobs, HT(ASCP)QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] negative controls on immunos
yes. I'm in the beginning stages of validating a new immuno machine. Because we have over 120 antibodies, I'm guessing about 2500 slides. Guess who pays for that testing? Certainly not CAP. I hate monopolies. Even though I know labs who dropped CAP and went with JCAHO, CAP is still a monopoly. Let's revolt. Seems to be catching on in the Middle East. Joe - Original Message - From: Angela Bitting akbitt...@geisinger.edu To: Diana McCaig dmcc...@ckha.on.ca; Greg Dobbin gvdob...@ihis.org; histonet@lists.utsouthwestern.edu; Joyce Weems jwe...@sjha.org Sent: Wednesday, March 02, 2011 10:42 AM Subject: RE: [Histonet] negative controls on immunos Fun no, impractical yes. Weems, Joyce jwe...@sjha.org 3/2/2011 10:30 AM The CAP guidelines are pretty clear. Copied from latest checklist.. Isn't this fun???! j:) ANP.22570 Phase IIN/A YES NO Are appropriate negative controls used? NOTE: Negative controls must assess the presence of nonspecific staining in patient tissue as well as the specificity of each antibody. Results of controls must be documented, either in internal laboratory records, or in the patient report. A statement in the report such as, All controls show appropriate reactivity is sufficient. A negative reagent control is used to assess nonspecific or aberrant staining in patient tissue related to the antigen retrieval conditions and/or detection system used. A separate section of patient tissue is processed using the same reagent and epitope retrieval protocol as the patient test slide, except that the primary antibody is omitted, and replaced by any one of the following: ■ An unrelated antibody of the same isotype as the primary antibody (for monoclonal primary antibodies) ■ An unrelated antibody from the same animal species as the primary antibody (for polyclonal primary antibodies) ■ The negative control reagent included in the staining kit ■ The diluent/buffer solution in which the primary antibody is diluted In general, a separate negative reagent control should be run for each block of patient tissue being immunostained; however, for cases in which there is simultaneous staining of multiple blocks from the same specimen with the same antibody (e.g., cytokeratin staining of multiple axillary sentinel lymph nodes), performing a single negative control on one of the blocks may be sufficient provided that all such blocks are fixed and processed identically. This exception does not apply to stains on different types of tissues or those using different antigen retrieval protocols or antibody detection systems. The laboratory director must determine which cases will have only one negative reagent control, and this must be specified in the department's procedure manual. The negative reagent control would ideally control for each reagent protocol and antibody retrieval condition; however, large antibody panels often employ multiple antigen retrieval procedures. In such cases, a reasonable minimum control would be to perform the negative reagent control using the most aggressive retrieval procedure in the particular antibody panel. Aggressiveness of antigen retrieval (in decreasing order) is as follows: pressure cooker; enzyme digestion, boiling; microwave; steamer; water bath. High pH retrieval should be considered more aggressive than comparable retrieval in citrate buffer at pH 6.0. It is also important to assess the specificity of each antibody by a negative tissue control, which must show no staining of tissues known to lack the antigen. The negative tissue control is processed using the same fixation, epitope retrieval and immunostaining protocols as the patient tissue. Unexpected positive staining of such tissues indicates that the test has lost specificity, perhaps because of improper antibody concentration or excessive antigen retrieval. Intrinsic properties of the test tissue may also be the cause of non-specific staining. For example, tissues with high endogenous biotin activity such as liver or renal tubules may simulate positive staining when using a detection method based on biotin labeling. A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks. These can provide simultaneous positive and negative tissue controls, and are considered best practice (see below). 2. The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody. 3. A separate negative tissue control slide. The type of negative tissue control used (i.e., separate sections, internal controls or multitissue blocks) should be specified in the laboratory manual (refer to ANP.22250). Multitissue blocks may be considered best practice and can have a major role in maintaining quality. When used as a combined positive and negative tissue control
Re: [Histonet] negative controls on immunos
Yes, it is always best to run every conceivable control available. Then you can be really, really, really sure. However, if you have practical issues that come into play such as cost of reagents, or extra controls taking up valuable space on the stainer then you might have to think about what you are trying to accomplish with each control and balance the cost vs benefit. So what are we checking with the negative control? We are checking the reagents to ensure that there is no non-specific reaction arising from the detection kit or the buffers, etc. If you are running the same block tomorrow with the same detection kit (ie same lot) it is not necessary (in my humble opinion) to check it again with another negative control. I suppose if you are worried that someone could be trying to sabotage your work by sneaking into your lab at night and contaminating your detection kit...then the simplest way to detect this sinister activity would be to run a negative control every time. Otherwise, I think one negative control slide per block per detection kit will be adequate. Disclaimer: Please know that I have just tried to inject a little humor into this response. I am really not trying to be sarcastic in anyway. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson Diana McCaig dmcc...@ckha.on.ca 3/1/2011 2:07 PM If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls on immunos
should be done before putting any antibody into diagnostic use. REFERENCES 1) Leong AS-Y, Cooper K, Leong FJW-M. Manual of Diagnostic Antibodies for Immunohistology. 2nd ed. London: Greenwich Medical Media; 2003 2) Dabbs DJ. Diagnostic Immunohistochemistry. New York: Churchill Livingstone; 2002 3) Burry RW. Specificity controls for immunocytochemical methods. J Histochem Cytochem 2000;48:163-166 Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Wednesday, March 02, 2011 09:15 To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] negative controls on immunos Yes, it is always best to run every conceivable control available. Then you can be really, really, really sure. However, if you have practical issues that come into play such as cost of reagents, or extra controls taking up valuable space on the stainer then you might have to think about what you are trying to accomplish with each control and balance the cost vs benefit.So what are we checking with the negative control? We are checking the reagents to ensure that there is no non-specific reaction arising from the detection kit or the buffers, etc. If you are running the same block tomorrow with the same detection kit (ie same lot) it is not necessary (in my humble opinion) to check it again with another negative control.I suppose if you are worried that someone could be trying to sabotage your work by sneaking into your lab at night and contaminating your detection kit...then the simplest way to detect this sinister activity would be to run a negative control every time. Otherwise, I think one negative control slide per block per detection kit will be adequate. Disclaimer: Please know that I have just tried to inject a little humor into this response. I am really not trying to be sarcastic in anyway. Cheers! GregGreg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson Diana McCaig dmcc...@ckha.on.ca 3/1/2011 2:07 PM If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls on immunos
are chosen appropriately, multitissue blocks may be used for many different primary antibodies, decreasing the number of different control blocks needed by the laboratory. Multitissue blocks are also ideal for determining optimal titers of primary antibodies since they allow simultaneous evaluation of many different pieces of tissue. Finally, they are a useful and efficient means to screen new antibodies for sensitivity and specificity or new lots of antibody for consistency, which should be done before putting any antibody into diagnostic use. REFERENCES 1) Leong AS-Y, Cooper K, Leong FJW-M. Manual of Diagnostic Antibodies for Immunohistology. 2nd ed. London: Greenwich Medical Media; 2003 2) Dabbs DJ. Diagnostic Immunohistochemistry. New York: Churchill Livingstone; 2002 3) Burry RW. Specificity controls for immunocytochemical methods. J Histochem Cytochem 2000;48:163-166 Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Wednesday, March 02, 2011 09:15 To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] negative controls on immunos Yes, it is always best to run every conceivable control available. Then you can be really, really, really sure. However, if you have practical issues that come into play such as cost of reagents, or extra controls taking up valuable space on the stainer then you might have to think about what you are trying to accomplish with each control and balance the cost vs benefit.So what are we checking with the negative control? We are checking the reagents to ensure that there is no non-specific reaction arising from the detection kit or the buffers, etc. If you are running the same block tomorrow with the same detection kit (ie same lot) it is not necessary (in my humble opinion) to check it again with another negative control.I suppose if you are worried that someone could be trying to sabotage your work by sneaking into your lab at night and contaminating your detection kit...then the simplest way to detect this sinister activity would be to run a negative control every time. Otherwise, I think one negative control slide per block per detection kit will be adequate. Disclaimer: Please know that I have just tried to inject a little humor into this response. I am really not trying to be sarcastic in anyway. Cheers! GregGreg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas JeffersonDiana McCaig dmcc...@ckha.on.ca 3/1/2011 2:07 PM If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually,diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ___ Histonet
RE: [Histonet] negative controls on immunos
for consistency, which should be done before putting any antibody into diagnostic use. REFERENCES 1) Leong AS-Y, Cooper K, Leong FJW-M. Manual of Diagnostic Antibodies for Immunohistology. 2nd ed. London: Greenwich Medical Media; 2003 2) Dabbs DJ. Diagnostic Immunohistochemistry. New York: Churchill Livingstone; 2002 3) Burry RW. Specificity controls for immunocytochemical methods. J Histochem Cytochem 2000;48:163-166 Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Wednesday, March 02, 2011 09:15 To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] negative controls on immunos Yes, it is always best to run every conceivable control available. Then you can be really, really, really sure. However, if you have practical issues that come into play such as cost of reagents, or extra controls taking up valuable space on the stainer then you might have to think about what you are trying to accomplish with each control and balance the cost vs benefit.So what are we checking with the negative control? We are checking the reagents to ensure that there is no non-specific reaction arising from the detection kit or the buffers, etc. If you are running the same block tomorrow with the same detection kit (ie same lot) it is not necessary (in my humble opinion) to check it again with another negative control.I suppose if you are worried that someone could be trying to sabotage your work by sneaking into your lab at night and contaminating your detection kit...then the simplest way to detect this sinister activity would be to run a negative control every time. Otherwise, I think one negative control slide per block per detection kit will be adequate. Disclaimer: Please know that I have just tried to inject a little humor into this response. I am really not trying to be sarcastic in anyway. Cheers! GregGreg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas JeffersonDiana McCaig dmcc...@ckha.on.ca 3/1/2011 2:07 PM If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually,diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
I run both positive and negative controls. Wherever possible, I put the positive control on the same slide as the test patient (this makes it easier for the pathologist and it also saves us the cost of on slide being tested). I always run a negative patient control on the exact same run. If I have more slides than will fit on one run, I will hold the specific case back, as I won't separate them. Matters not if there is more than one machine, the case will stay together. As far as negatives, I will run an extra negative if my run has both poly and mono antibodies. So, there are many times, I have two negatives running per case. I run only one detection system, so I don't have that variable to deal with. Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Friday, October 15, 2010 12:50 PM To: Victoria Baker; Histo Net list server Subject: RE: [Histonet] negative controls Hi Vikki, I have 1 Ventana XT and 3 Ultras. I have certain antibodies designated to each ultra. This means not all slides from any case are guaranteed to be on the same machine. They obviously all have their own detection kit. My theory is that our detection kits are QCd prior to initial load onto an instrument. The patient block was treated the same prior to cutting. The slides receive solutions that are QCd...I think we are covered. What are your thoughts? Melissa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 10:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/13/10 18:34:00 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] negative controls
To my knowledge for this stain to be legitimate and meet criteria you must run your positive and negative controls on the same run. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Victoria Baker bakevicto...@gma il.comTo Sent by: Histo Net list server histonet-bounces@ HistoNet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] negative controls 10/15/2010 10:25 AM Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
Pretty sure you have to run your positive with your negative to keep the conditions 100% the same. You don't have to put them on the same slide necessarily, but they need to be on the same run. Also, say you are running 15 HP patient slides, you can have one negative control for all of these as long as it is on the same run. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 Original Message Subject: [Histonet] negative controls From: Victoria Baker bakevicto...@gmail.com Date: Fri, October 15, 2010 7:25 am To: Histo Net list server HistoNet@lists.utsouthwestern.edu Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] negative controls
Hi Vikki, It's best to run it all together and run a negative control for each detection kit. Mark On Fri, Oct 15, 2010 at 7:25 AM, Victoria Baker bakevicto...@gmail.comwrote: Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
We run negative controls on every block of a case within the same run. On autopsy cases, we only run 1 negative per tissue type, within the same run...this is the only exception to the rule of 1 negative per block. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 9:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
Why do you need a negative control for each block if you are runn= ing
the same antibody on each patient block? Is it just for case by c ase
reference so the negative is filed with the patient slide? Why
co= uldn't you have a control slide bank that was dated so all the
slides you d= id on that day, on that run, could be referenced back to
that control? = ; Just curious?
Sarah Goebel, B.A., HT (ASCP)
Histotechnician= br
XBiotech USA Inc.
8201 East Riverside Dr. Bld= g 4 Suite 100
Austin, Texas 78744
(512)386-= 5107
Original Message
Subject: RE: [Histonet] negative controls
From: Sebree Linda A [1]lseb...@= uwhealth.org
Date: Fri, October 15, 2010 8:08 am
To: Victoria Baker [2]bakevict= o...@gmail.com, Histo Net list
server
[3]histo...@lists.uts= outhwestern.edu
We run negative controls on every block of a case within the same run.
On autopsy cases, we only run 1 negative per tissue type, within the
same run...this is the only exception to the rule of 1 negative per
block.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: [4]histonet= -boun...@lists.utsouthwestern.edu
[[5]mailto:histon= et-boun...@lists.utsouthwestern.edu] On Behalf Of
Victoria
Baker
Sent: Friday, October 15, 2010 9:26 AM
To: Histo Net list server
Subject: [Histonet] negative controls
Hi
I have a hypothetical question to those who run IHC on Ventana
instruments.
Are you running your negatives with your patient/test cases or on a
separate
run? Also, if you are doing this and have to use a different detection
kit
how do you work the QA/QC portion of this for CAP requirements.
Thanks
Vikki
___
Histonet mailing list
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[7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
[8]histo...@lists.utsouth= western.edu
[9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. 3Dmailto:lseb...@uwhealth.org;
2. 3Dmailto:bakevicto...@gmail.com;
3. 3Dmailto:HistoNet@lists.utsouthwestern.edu;
4. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu;
5. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu;
6. 3Dmailto:Histonet@lists.utsouthwestern.edu;
7. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
8. 3Dmailto:Histonet@lists.utsouthwestern.edu;
9. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
Because each tissue block has its own characteristics regarding fixation and processing some of which can influence the reactivity. If you have a bank of negative controls, how can you be sure that any of those blocks have received exactly the same treatment and reacted in the same way to the test block? The same goes for any bank of positives, so that is why you should have a positive control section in the same slide as the test section. René J. --- On Fri, 10/15/10, sgoe...@xbiotech.com sgoe...@xbiotech.com wrote: From: sgoe...@xbiotech.com sgoe...@xbiotech.com Subject: RE: [Histonet] negative controls To: Sebree Linda A lseb...@uwhealth.org Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu Date: Friday, October 15, 2010, 11:17 AM Why do you need a negative control for each block if you are runn= ing the same antibody on each patient block? Is it just for case by c ase reference so the negative is filed with the patient slide? Why co= uldn't you have a control slide bank that was dated so all the slides you d= id on that day, on that run, could be referenced back to that control? = ; Just curious? Sarah Goebel, B.A., HT (ASCP) Histotechnician= br XBiotech USA Inc. 8201 East Riverside Dr. Bld= g 4 Suite 100 Austin, Texas 78744 = (512)386-= 5107 Original Message Subject: RE: [Histonet] negative controls From: Sebree Linda A [1]lseb...@= uwhealth.org Date: Fri, October 15, 2010 8:08 am To: Victoria Baker [2]bakevict= o...@gmail.com, Histo Net list server [3]histo...@lists.uts= outhwestern.edu We run negative controls on every block of a case within the same run. On autopsy cases, we only run 1 negative per tissue type, within the same run...this is the only exception to the rule of 1 negative per block. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: [4]histonet= -boun...@lists.utsouthwestern.edu [[5]mailto:histon= et-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 9:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki ___ Histonet mailing list [6]histo...@lists.utsouth= western.edu [7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list [8]histo...@lists.utsouth= western.edu [9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3Dmailto:lseb...@uwhealth.org; 2. 3Dmailto:bakevicto...@gmail.com; 3. 3Dmailto:HistoNet@lists.utsouthwestern.edu; 4. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu; 5. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu; 6. 3Dmailto:Histonet@lists.utsouthwestern.edu; 7. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet; 8. 3Dmailto:Histonet@lists.utsouthwestern.edu; 9. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet; ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
So for every HP you do, you process a control cassette with the patient
tissue cassette? That seems like alot? How do you get that many
control tissues on a daily basis? What do you do with the remaining
tissue in the control block? If you throw them away everyday, I would
be interested in some of them. How do you know what IHC stains the
pathologist is going to order to know what control tissue to fix and
process at the exact same time? We have always just had a bunch of
blocks that you cut a control from? I understand that there is
variability with processing, age, etc. not trying to be dense just still
don't understand... Most places I have ever worked have control blocks
that they cut a fresh control from everyday, then stain with the patient
tissue. If there are 3 HP cases, from what I am understanding, you guys
are saying you need 3 controls for slides that are on the same machine,
with the same reagents, same antibody, and same times. Why couldn't you
just have one for all 3 cases? Then the next day have a fresh ONE for
that day, date them, and file them. So if you needed to see the HP
control for October 15th, you could go pull the control for that day...
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: RE: [Histonet] negative controls
From: Rene J Buesa rjbu...@yahoo.com
Date: Fri, October 15, 2010 8:33 am
To: Sebree Linda A lseb...@uwhealth.org, sgoe...@xbiotech.com
Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu
Because each tissue block has its own characteristics regarding fixation
and processing some of which can influence the reactivity. If you have a
bank of negative controls, how can you be sure that any of those blocks
have received exactly the same treatment and reacted in the same way to
the test block?
The same goes for any bank of positives, so that is why you should have
a positive control section in the same slide as the test section.
René J.
--- On Fri, 10/15/10, sgoe...@xbiotech.com sgoe...@xbiotech.com wrote:
From: sgoe...@xbiotech.com sgoe...@xbiotech.com
Subject: RE: [Histonet] negative controls
To: Sebree Linda A lseb...@uwhealth.org
Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu
Date: Friday, October 15, 2010, 11:17 AM
Why do you need a negative control for each block if you are runn=
ing
the same antibody on each patient block? Is it just for case by c
ase reference so the negative is filed with the patient slide? Why
co= uldn't you have a control slide bank that was dated so all
the
slides you d= id on that day, on that run, could be referenced back
to
that control? = ; Just curious?
Sarah Goebel, B.A., HT (ASCP)
Histotechnician= br
XBiotech USA Inc.
8201 East Riverside Dr. Bld= g 4 Suite 100
Austin, Texas 78744
=
(512)386-= 5107
Original Message
Subject: RE: [Histonet] negative controls
From: Sebree Linda A [1]lseb...@= uwhealth.org
Date: Fri, October 15, 2010 8:08 am
To: Victoria Baker [2]bakevict= o...@gmail.com, Histo Net
list
server
[3]histo...@lists.uts= outhwestern.edu
We run negative controls on every block of a case within the same
run.
On autopsy cases, we only run 1 negative per tissue type, within the
same run...this is the only exception to the rule of 1 negative per
block.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: [4]histonet= -boun...@lists.utsouthwestern.edu
[[5]mailto:histon= et-boun...@lists.utsouthwestern.edu] On Behalf
Of
Victoria
Baker
Sent: Friday, October 15, 2010 9:26 AM
To: Histo Net list server
Subject: [Histonet] negative controls
Hi
I have a hypothetical question to those who run IHC on Ventana
instruments.
Are you running your negatives with your patient/test cases or on a
separate
run? Also, if you are doing this and have to use a different
detection
kit
how do you work the QA/QC portion of this for CAP requirements.
Thanks
Vikki
___
Histonet mailing list
[6]histo...@lists.utsouth= western.edu
[7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
[8]histo...@lists.utsouth= western.edu
[9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. 3Dmailto:lseb...@uwhealth.org;
2. 3Dmailto:bakevicto...@gmail.com;
3. 3Dmailto:HistoNet@lists.utsouthwestern.edu;
4. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu;
5. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu;
6. 3Dmailto:Histonet@lists.utsouthwestern.edu;
7. 3Dhttp://lists.utsouthwestern.edu/mailman
Re: [Histonet] negative controls
Hi Sarah,
It's better to have the control on the same slide. There are slides that
work and slides that don't. You know which ones are good and which ones are
bad because you have that control on each slide. It's not always a complete
run that fails. Yes, you CAN have a single batch control (it's not against
the rules), but best practice is to have a control on each slide.
You don't process a control for each case, you use a similarly processed
piece of positive tissue placed on each slide (except the negative which
should just have the patient tissue).
Mark
On Fri, Oct 15, 2010 at 8:47 AM, sgoe...@xbiotech.com wrote:
So for every HP you do, you process a control cassette with the patient
tissue cassette? That seems like alot? How do you get that many
control tissues on a daily basis? What do you do with the remaining
tissue in the control block? If you throw them away everyday, I would
be interested in some of them. How do you know what IHC stains the
pathologist is going to order to know what control tissue to fix and
process at the exact same time? We have always just had a bunch of
blocks that you cut a control from? I understand that there is
variability with processing, age, etc. not trying to be dense just still
don't understand... Most places I have ever worked have control blocks
that they cut a fresh control from everyday, then stain with the patient
tissue. If there are 3 HP cases, from what I am understanding, you guys
are saying you need 3 controls for slides that are on the same machine,
with the same reagents, same antibody, and same times. Why couldn't you
just have one for all 3 cases? Then the next day have a fresh ONE for
that day, date them, and file them. So if you needed to see the HP
control for October 15th, you could go pull the control for that day...
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: RE: [Histonet] negative controls
From: Rene J Buesa rjbu...@yahoo.com
Date: Fri, October 15, 2010 8:33 am
To: Sebree Linda A lseb...@uwhealth.org, sgoe...@xbiotech.com
Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu
Because each tissue block has its own characteristics regarding fixation
and processing some of which can influence the reactivity. If you have a
bank of negative controls, how can you be sure that any of those blocks
have received exactly the same treatment and reacted in the same way to
the test block?
The same goes for any bank of positives, so that is why you should have
a positive control section in the same slide as the test section.
René J.
--- On Fri, 10/15/10, sgoe...@xbiotech.com sgoe...@xbiotech.com wrote:
From: sgoe...@xbiotech.com sgoe...@xbiotech.com
Subject: RE: [Histonet] negative controls
To: Sebree Linda A lseb...@uwhealth.org
Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu
Date: Friday, October 15, 2010, 11:17 AM
Why do you need a negative control for each block if you are runn=
ing
the same antibody on each patient block? Is it just for case by c
ase reference so the negative is filed with the patient slide? Why
co= uldn't you have a control slide bank that was dated so all
the
slides you d= id on that day, on that run, could be referenced back
to
that control? = ; Just curious?
Sarah Goebel, B.A., HT (ASCP)
Histotechnician= br
XBiotech USA Inc.
8201 East Riverside Dr. Bld= g 4 Suite 100
Austin, Texas 78744
=
(512)386-= 5107
Original Message
Subject: RE: [Histonet] negative controls
From: Sebree Linda A [1]lseb...@= uwhealth.org
Date: Fri, October 15, 2010 8:08 am
To: Victoria Baker [2]bakevict= o...@gmail.com, Histo Net
list
server
[3]histo...@lists.uts= outhwestern.edu
We run negative controls on every block of a case within the same
run.
On autopsy cases, we only run 1 negative per tissue type, within the
same run...this is the only exception to the rule of 1 negative per
block.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: [4]histonet= -boun...@lists.utsouthwestern.edu
[[5]mailto:histon= et-boun...@lists.utsouthwestern.edu] On Behalf
Of
Victoria
Baker
Sent: Friday, October 15, 2010 9:26 AM
To: Histo Net list server
Subject: [Histonet] negative controls
Hi
I have a hypothetical question to those who run IHC on Ventana
instruments.
Are you running your negatives with your patient/test cases or on a
separate
run? Also, if you are doing this and have to use a different
detection
kit
how do you work the QA/QC portion of this for CAP requirements.
Thanks
Vikki
RE: [Histonet] negative controls
We're talking NEGATIVE controls here folks and if you use internal tissue for your positive and negative controls...they should all be processed and blocked in the same fashion as your actual patient material. We have scrutinized the negative control issue on the CAP checklist and have decided that if tissue warrants it, we cut an extra patient slide and it is run as the negative for that case. All is processed the same and stained identically with exception of the primary antibody. If there is not adequate material we use the Positive control and treat it negatively. This is reflected in our policy and procedure. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 sgoe...@xbiotech .com Sent by: To histonet-bounces@ Rene J Buesa rjbu...@yahoo.com lists.utsouthwest cc ern.edu Histo Net list server HistoNet@lists.utsouthwestern.edu , Sebree Linda A 10/15/2010 11:47 lseb...@uwhealth.org AMSubject RE: [Histonet] negative controls So for every HP you do, you process a control cassette with the patient tissue cassette? That seems like alot? How do you get that many control tissues on a daily basis? What do you do with the remaining tissue in the control block? If you throw them away everyday, I would be interested in some of them. How do you know what IHC stains the pathologist is going to order to know what control tissue to fix and process at the exact same time? We have always just had a bunch of blocks that you cut a control from? I understand that there is variability with processing, age, etc. not trying to be dense just still don't understand... Most places I have ever worked have control blocks that they cut a fresh control from everyday, then stain with the patient tissue. If there are 3 HP cases, from what I am understanding, you guys are saying you need 3 controls for slides that are on the same machine, with the same reagents, same antibody, and same times. Why couldn't you just have one for all 3 cases? Then the next day have a fresh ONE for that day, date them, and file them. So if you needed to see the HP control for October 15th, you could go pull the control for that day... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 Original Message Subject: RE: [Histonet] negative controls From: Rene J Buesa rjbu...@yahoo.com Date: Fri, October 15, 2010 8:33 am To: Sebree Linda A lseb...@uwhealth.org, sgoe...@xbiotech.com Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu Because each tissue block has its own characteristics regarding fixation and processing some of which can influence the reactivity. If you have a bank of negative controls, how can you be sure that any of those blocks have received exactly the same treatment and reacted in the same way to the test block? The same goes for any bank of positives, so that is why you should have a positive control section in the same slide as the test section. René J. --- On Fri, 10/15/10, sgoe...@xbiotech.com sgoe...@xbiotech.com wrote: From: sgoe...@xbiotech.com sgoe...@xbiotech.com Subject: RE: [Histonet] negative controls To: Sebree Linda A lseb...@uwhealth.org Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu Date: Friday, October 15, 2010, 11:17 AM Why do you need a negative control for each block if you are runn= ing the same antibody on each patient block? Is it just for case by c ase reference so the negative is filed with the patient slide? Why co= uldn't you have a control slide bank that was dated so all the slides you d= id on that day, on that run, could be referenced back to that control? = ; Just curious? Sarah Goebel, B.A., HT (ASCP) Histotechnician= br XBiotech USA Inc. 8201 East Riverside Dr. Bld= g 4 Suite 100 Austin, Texas 78744 = (512)386-= 5107
RE: [Histonet] negative controls
To clarify even further: we cut 2 patient sections, put one on a slide with a
section of positive control already on it. This slide gets stained with the
antibody The other section goes on another slide and is run as a corresponding
negative control using the same antibody protocol but substituting a negative
control serum for the antibody, thus this is a negative reagent control
slide. Elements within the patient slide that received antibody and are
expected to be negative, serve as a negative tissue control. Again, we run 1
negative control slide for EVERY 1 patient block in a run but only 1 negative
control per any number of antibodies run on that same block, using the harshest
protocol. Only autopsy cases differ in that we run 1 negative control per
TISSUE TYPE.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: sgoe...@xbiotech.com [mailto:sgoe...@xbiotech.com]
Sent: Friday, October 15, 2010 10:48 AM
To: Rene J Buesa
Cc: Histo Net list server; Sebree Linda A
Subject: RE: [Histonet] negative controls
So for every HP you do, you process a control cassette with the patient
tissue cassette? That seems like alot? How do you get that many
control tissues on a daily basis? What do you do with the remaining
tissue in the control block? If you throw them away everyday, I would
be interested in some of them. How do you know what IHC stains the
pathologist is going to order to know what control tissue to fix and
process at the exact same time? We have always just had a bunch of
blocks that you cut a control from? I understand that there is
variability with processing, age, etc. not trying to be dense just still
don't understand... Most places I have ever worked have control blocks
that they cut a fresh control from everyday, then stain with the patient
tissue. If there are 3 HP cases, from what I am understanding, you guys
are saying you need 3 controls for slides that are on the same machine,
with the same reagents, same antibody, and same times. Why couldn't you
just have one for all 3 cases? Then the next day have a fresh ONE for
that day, date them, and file them. So if you needed to see the HP
control for October 15th, you could go pull the control for that day...
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: RE: [Histonet] negative controls
From: Rene J Buesa rjbu...@yahoo.com
Date: Fri, October 15, 2010 8:33 am
To: Sebree Linda A lseb...@uwhealth.org, sgoe...@xbiotech.com
Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu
Because each tissue block has its own characteristics regarding fixation
and processing some of which can influence the reactivity. If you have a
bank of negative controls, how can you be sure that any of those blocks
have received exactly the same treatment and reacted in the same way to
the test block?
The same goes for any bank of positives, so that is why you should have
a positive control section in the same slide as the test section.
René J.
--- On Fri, 10/15/10, sgoe...@xbiotech.com sgoe...@xbiotech.com wrote:
From: sgoe...@xbiotech.com sgoe...@xbiotech.com
Subject: RE: [Histonet] negative controls
To: Sebree Linda A lseb...@uwhealth.org
Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu
Date: Friday, October 15, 2010, 11:17 AM
Why do you need a negative control for each block if you are runn=
ing
the same antibody on each patient block? Is it just for case by c
ase reference so the negative is filed with the patient slide? Why
co= uldn't you have a control slide bank that was dated so all
the
slides you d= id on that day, on that run, could be referenced back
to
that control? = ; Just curious?
Sarah Goebel, B.A., HT (ASCP)
Histotechnician= br
XBiotech USA Inc.
8201 East Riverside Dr. Bld= g 4 Suite 100
Austin, Texas 78744
=
(512)386-= 5107
Original Message
Subject: RE: [Histonet] negative controls
From: Sebree Linda A [1]lseb...@= uwhealth.org
Date: Fri, October 15, 2010 8:08 am
To: Victoria Baker [2]bakevict= o...@gmail.com, Histo Net
list
server
[3]histo...@lists.uts= outhwestern.edu
We run negative controls on every block of a case within the same
run.
On autopsy cases, we only run 1 negative per tissue type, within the
same run...this is the only exception to the rule of 1 negative per
block.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: [4]histonet= -boun...@lists.utsouthwestern.edu
[[5]mailto:histon= et-boun...@lists.utsouthwestern.edu] On Behalf
Of
Victoria
Baker
Sent: Friday
RE: [Histonet] negative controls
Beatrice, If there is not adequate tissue to cut a negative control, the pathologist has to make a priority decision so as to allow 1 slide to be treated as the negative control. If for some reason, there is only 1 slide available, i.e. no slide available to use as a negative control, we do not run the test. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: bsulli...@shorememorial.org [mailto:bsulli...@shorememorial.org] Sent: Friday, October 15, 2010 11:01 AM To: sgoe...@xbiotech.com Cc: Histo Net list server; histonet-boun...@lists.utsouthwestern.edu; Sebree Linda A; Rene J Buesa Subject: RE: [Histonet] negative controls We're talking NEGATIVE controls here folks and if you use internal tissue for your positive and negative controls...they should all be processed and blocked in the same fashion as your actual patient material. We have scrutinized the negative control issue on the CAP checklist and have decided that if tissue warrants it, we cut an extra patient slide and it is run as the negative for that case. All is processed the same and stained identically with exception of the primary antibody. If there is not adequate material we use the Positive control and treat it negatively. This is reflected in our policy and procedure. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 sgoe...@xbiotech .com Sent by: To histonet-bounces@ Rene J Buesa rjbu...@yahoo.com lists.utsouthwest cc ern.edu Histo Net list server HistoNet@lists.utsouthwestern.edu , Sebree Linda A 10/15/2010 11:47 lseb...@uwhealth.org AMSubject RE: [Histonet] negative controls So for every HP you do, you process a control cassette with the patient tissue cassette? That seems like alot? How do you get that many control tissues on a daily basis? What do you do with the remaining tissue in the control block? If you throw them away everyday, I would be interested in some of them. How do you know what IHC stains the pathologist is going to order to know what control tissue to fix and process at the exact same time? We have always just had a bunch of blocks that you cut a control from? I understand that there is variability with processing, age, etc. not trying to be dense just still don't understand... Most places I have ever worked have control blocks that they cut a fresh control from everyday, then stain with the patient tissue. If there are 3 HP cases, from what I am understanding, you guys are saying you need 3 controls for slides that are on the same machine, with the same reagents, same antibody, and same times. Why couldn't you just have one for all 3 cases? Then the next day have a fresh ONE for that day, date them, and file them. So if you needed to see the HP control for October 15th, you could go pull the control for that day... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 Original Message Subject: RE: [Histonet] negative controls From: Rene J Buesa rjbu...@yahoo.com Date: Fri, October 15, 2010 8:33 am To: Sebree Linda A lseb...@uwhealth.org, sgoe...@xbiotech.com Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu Because each tissue block has its own characteristics regarding fixation and processing some of which can influence the reactivity. If you have a bank of negative controls, how can you be sure that any of those blocks have received exactly the same treatment and reacted in the same way to the test block? The same goes for any bank of positives, so that is why you should have a positive control section in the same slide as the test section. René J. --- On Fri, 10/15/10, sgoe...@xbiotech.com sgoe
RE: [Histonet] negative controls
Hi Vikki, I have 1 Ventana XT and 3 Ultras. I have certain antibodies designated to each ultra. This means not all slides from any case are guaranteed to be on the same machine. They obviously all have their own detection kit. My theory is that our detection kits are QCd prior to initial load onto an instrument. The patient block was treated the same prior to cutting. The slides receive solutions that are QCd...I think we are covered. What are your thoughts? Melissa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 10:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] negative controls
Linda, My system of negative controls is identical to yours. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Sebree Linda A lseb...@uwhealth.org To: sgoe...@xbiotech.com; Rene J Buesa rjbu...@yahoo.com Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu Sent: Friday, October 15, 2010 11:07 AM Subject: RE: [Histonet] negative controls To clarify even further: we cut 2 patient sections, put one on a slide with a section of positive control already on it. This slide gets stained with the antibody The other section goes on another slide and is run as a corresponding negative control using the same antibody protocol but substituting a negative control serum for the antibody, thus this is a negative reagent control slide. Elements within the patient slide that received antibody and are expected to be negative, serve as a negative tissue control. Again, we run 1 negative control slide for EVERY 1 patient block in a run but only 1 negative control per any number of antibodies run on that same block, using the harshest protocol. Only autopsy cases differ in that we run 1 negative control per TISSUE TYPE. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: sgoe...@xbiotech.com [mailto:sgoe...@xbiotech.com] Sent: Friday, October 15, 2010 10:48 AM To: Rene J Buesa Cc: Histo Net list server; Sebree Linda A Subject: RE: [Histonet] negative controls So for every HP you do, you process a control cassette with the patient tissue cassette? That seems like alot? How do you get that many control tissues on a daily basis? What do you do with the remaining tissue in the control block? If you throw them away everyday, I would be interested in some of them. How do you know what IHC stains the pathologist is going to order to know what control tissue to fix and process at the exact same time? We have always just had a bunch of blocks that you cut a control from? I understand that there is variability with processing, age, etc. not trying to be dense just still don't understand... Most places I have ever worked have control blocks that they cut a fresh control from everyday, then stain with the patient tissue. If there are 3 HP cases, from what I am understanding, you guys are saying you need 3 controls for slides that are on the same machine, with the same reagents, same antibody, and same times. Why couldn't you just have one for all 3 cases? Then the next day have a fresh ONE for that day, date them, and file them. So if you needed to see the HP control for October 15th, you could go pull the control for that day... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 Original Message Subject: RE: [Histonet] negative controls From: Rene J Buesa rjbu...@yahoo.com Date: Fri, October 15, 2010 8:33 am To: Sebree Linda A lseb...@uwhealth.org, sgoe...@xbiotech.com Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu Because each tissue block has its own characteristics regarding fixation and processing some of which can influence the reactivity. If you have a bank of negative controls, how can you be sure that any of those blocks have received exactly the same treatment and reacted in the same way to the test block? The same goes for any bank of positives, so that is why you should have a positive control section in the same slide as the test section. René J. --- On Fri, 10/15/10, sgoe...@xbiotech.com sgoe...@xbiotech.com wrote: From: sgoe...@xbiotech.com sgoe...@xbiotech.com Subject: RE: [Histonet] negative controls To: Sebree Linda A lseb...@uwhealth.org Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu Date: Friday, October 15, 2010, 11:17 AM Why do you need a negative control for each block if you are runn= ing the same antibody on each patient block? Is it just for case by c ase reference so the negative is filed with the patient slide? Why co= uldn't you have a control slide bank that was dated so all the slides you d= id on that day, on that run, could be referenced back to that control? = ; Just curious? Sarah Goebel, B.A., HT (ASCP) Histotechnician= br XBiotech USA Inc. 8201 East Riverside Dr. Bld= g 4 Suite 100 Austin, Texas 78744
RE: [Histonet] Negative controls
So if one is doing an immunostaining staining run with say, 10 different primary antibodies, at different dilutions, would you have the the same number of different dilutions of the Biocare universal control, or just have the one at the least dilute, say 1/10??, thanks. Richard Edwards Leicester UniversityU.K.. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Sally A Sent: 04 March 2010 16:02 To: Histonet Subject: RE: [Histonet] Negative controls Yes, Linda and I select the harshest protocol in the list of antibodies we're running per case-we figure that the harshest will produce the biggest problems with background, etc. Sally Ann Drew, MT(ASCP) IHC/ISH Clinical Research Laboratory UWHC 600 Highland Ave. DB1-223, Mail Code 3224 Madison, WI 53792 (608)265-6596 Fax:(608)262-7174 Hi Anita, We are currently examining the negative control issue in our laboratory. We also have Ventanas. We currently use a normal goat serum as our non-immune serum. We are considering changing our SOP's to use the Ventana Neg Control Rabbit and Neg Control Mouse in dispensers. Linda's suggestion of Biocare's Universal Negative Control Serum is intriguing. It would seem regressive to go back to the days of delineating mouse/rabbit antisera, using separate dispensers for each, and a universal negative seems to be the way to go, especially when using universal DAB kits. Using such a reagent would mean it could be applied to each case's negative control slide. Linda or others, would such a negative control serum protocol include your most aggressive pretreatment? Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative controls
We too use the Universal negative from Biocare, and it has passed through 2 CAP inspections so no problem on that front. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Thursday, March 04, 2010 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative controls Hi Anita, We are currently examining the negative control issue in our laboratory. We also have Ventanas. We currently use a normal goat serum as our non-immune serum. We are considering changing our SOP's to use the Ventana Neg Control Rabbit and Neg Control Mouse in dispensers. Linda's suggestion of Biocare's Universal Negative Control Serum is intriguing. It would seem regressive to go back to the days of delineating mouse/rabbit antisera, using separate dispensers for each, and a universal negative seems to be the way to go, especially when using universal DAB kits. Using such a reagent would mean it could be applied to each case's negative control slide. Linda or others, would such a negative control serum protocol include your most aggressive pretreatment? Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative controls
Yes, Linda and I select the harshest protocol in the list of antibodies we're running per case-we figure that the harshest will produce the biggest problems with background, etc. Sally Ann Drew, MT(ASCP) IHC/ISH Clinical Research Laboratory UWHC 600 Highland Ave. DB1-223, Mail Code 3224 Madison, WI 53792 (608)265-6596 Fax:(608)262-7174 Hi Anita, We are currently examining the negative control issue in our laboratory. We also have Ventanas. We currently use a normal goat serum as our non-immune serum. We are considering changing our SOP's to use the Ventana Neg Control Rabbit and Neg Control Mouse in dispensers. Linda's suggestion of Biocare's Universal Negative Control Serum is intriguing. It would seem regressive to go back to the days of delineating mouse/rabbit antisera, using separate dispensers for each, and a universal negative seems to be the way to go, especially when using universal DAB kits. Using such a reagent would mean it could be applied to each case's negative control slide. Linda or others, would such a negative control serum protocol include your most aggressive pretreatment? Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
Anita, If you're referring to the reagents you use, we use a Universal Negative Control Serum (Biocare) on our negative control slides. We have 3 Ventana instruments and have never been cited. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, March 03, 2010 11:44 AM To: histo...@pathology.swmed.edu Subject: [Histonet] negative controls we have a ventana benchmark and use rabbit and mouse negative controls, is there anyone out there that uses a universal control and is that ok with your cap inspection? thanks so much antia dudley providence hosp mobile alabama _ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative controls for special stains (non-organism)?!?!
I don't see how negative controls for ordinary histochemical procedures would be much help unless they are the same tissue as the test sample. If you are doing a congo red for amyloid plaques in brain, I can see using a known negative brain as a control. But I don't see the point of running a brain section as a negative collagen control when you are staining a lung. What relevance would that have to the test section? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
