[ccp4bb] cryoprotectant for crystals in isopropanol

Dear all, The question is regarding the cryoprotectant.We have crystallized a protein in 20% PEG 500 with 10% isopropanol.What should be the cryo we should try for data collection.Also,what percentage of PEG 500 is sufficient enough as a cryo for data collection. We are also trying to crystallize

Re: [ccp4bb] Broken Masks

I think it is a version bug of some sort - when I ran the same mask creation run in CCP4i on my MacBook laptop (which has the most recent CCP4) it displays fine and also worked perfectly in dm averaging, turning a tangled mess into interpretable density. The mask generated by NCSMASK on my

[ccp4bb] The Lawnmower Man

Dear everyone, Has anybody tried to hook up a protein display/model building program/computer with 3D glasses of the type people are developing for games? The type of glasses where one obviates the use of large monitors altogether, that is the display sits on the glasses themselves. Thanks,

Re: [ccp4bb] The Lawnmower Man

Wow, that is like, so last week(!). I'm using COOT though a wireless interface to a head-up-display mounted inside my contact lenses, controlled via a neural lace* embedded directly into my parietal lobe. I can refine and rebuild my structure whilst doing minipreps, having a coffee, in the

[ccp4bb] Post-Doctoral position at the University of Dundee

UNIVERSITY OF DUNDEE COLLEGE OF LIFE SCIENCES DIVISION OF BIOLOGICAL CHEMISTRY AND DRUG DISCOVERY POSTDOCTORAL RESEARCH ASSISTANT GRADE 7 (£26,666 - £32,796) Enthusiastic, highly motivated individuals are sought to study key enzymes of microbial pathogens to advance fundamental understanding of

Re: [ccp4bb] cryoprotectant for crystals in isopropanol

Not that all proteins are created alike, but we had a protein crystallize in a similar situation (PEG/20%IPA). I don't remember the exact details, but we did have good luck using just glycerol as a cryoprotectant. That is, I made up the well buffer and added to that well buffer 20-25%

[ccp4bb] PRODRG?

BSD Hi all, Does anybody know what happened to the PRODRG server web site? Thanks Harry - Harry M. Greenblatt Staff Scientist Dept of Structural Biology [EMAIL PROTECTED] Weizmann Institute of Science

[ccp4bb] Workshop on Raw Image Formats in Structural Biology

There will be a workshop on Raw Image Formats in Structural Biology to be held at Brookhaven National Laboratory on 22 May 2008. Over the past 2 years, imgCIF has seen increasing use, and the interactions among raw image formats for x-ray crystallography, neutron crystallography and microscopy

[ccp4bb] Crystal scoring sheets for iPhones/Pocket-PCs

Hi, I could be wrong, but I’m not aware of any servers that have tray inspection web pages designed for iPhones and Pocket-PCs, so I thought I’d try and make a web page with simple scoring sheets for such devices. I don’t own an iPhone myself, but the following web page seems to work well on

Re: [ccp4bb] cryoprotectant for crystals in isopropanol

Another anecdote for you: I had crystals that grew in 15% PEG 2000 and 15% isopropanol. Cryos with glycerol melted the crystals. The best cryo I found was with 15% PEG 2000, 15% isopropanol, and 20% PEG 400. It's hard to predict how your crystals will behave with different cryoprotectants--

[ccp4bb] Senior Scientist Position at Evotec

Apologies for the slightly off topic post but can I draw people's attention to the job advert below. Due to continued expansion Evotec has a post for a Senior Scientist to join the X-ray crystallography team based in Abingdon, UK. Working within a small multidisciplinary project team your task

Re: [ccp4bb] Crystal scoring sheets for iPhones/Pocket-PCs

Sounds great. You can jailbreak an iPhone/iPod touch and install a whole BSD subsystem on it. So printing should be possible at some point... This of course also suggests that iPod touch is a valid research expense ... William G. Scott Contact info: http://chemistry.ucsc.edu/~wgscott/

[ccp4bb] Phased Anomalous Difference Map

Hi All, Can anyone tell me how I calculate a phased anomalous difference map? I want to confirm such as the presence of Cl or S ion in an unknown electron density. Rajan.

Re: [ccp4bb] Crystal scoring sheets for iPhones/Pocket-PCs

Toys for crystallography: Maybe this could be filed under the same category as Johnny Chung Lee's Wii Remote projects a number of people here have mentioned about (though this certainly isn’t as cool as his “Minority Report” gloves or fake 3D-glasses)! Still, if Apple has it's way, in a year

Re: [ccp4bb] PRODRG?

Yes, it is down at the moment, due to a hacking event apparently. Don't know any more. Martyn -Original Message- From: CCP4 bulletin board on behalf of Harry M. Greenblatt Sent: Wed 3/19/2008 1:28 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] PRODRG? BSD Hi all, Does anybody know

Re: [ccp4bb] The Lawnmower Man

Neural lace embedding is too barbaric. Has anyone written a coot driver for this non-invasive alternative: http://techreport.com/discussions.x/13928 ? The superiority of thought driven building over dragging a mouse + modifier keys might be reason enough to get me to switch from O.

[ccp4bb] Back-Fourier transforming an EM map with SFALL

Hello All, I'm trying to back-FT an EM map to produce Fs and phases to use in molecular replacement, but am having a problem with running SFALL. Here's the issue: When running the back-transform in SFALL (generating structure factors from a map), I get the following error: Incorrect sampling