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Hello.
I am working with a protein that turns a yellowish-brown color when it is
concentrated to around 2 mg/ml or higher in a small volume (a few hundred
uL). I was wondering if the protein bound a metal or other prosthetic
group that would give it this color? The protein's color somewhat
Hi Matt,
to check, if the color comes from a metal ion, you can get an AAS
analysis done. You need something in the range of 1 mg of well dialyzed
protein (metal free buffer!). We got an analysis done for the most
important metals (Mn, Fe, Co, Ni, Cu, Zn) for ca. 20 Eur per element
from a
I am working with a protein that turns a yellowish-brown color when it is
concentrated to around 2 mg/ml or higher in a small volume (a few hundred
uL). I was wondering if the protein bound a metal or other prosthetic
group that would give it this color? The protein's color somewhat
Matthew Alan Bratkowski wrote:
Hello.
I am working with a protein that turns a yellowish-brown color when it is
concentrated to around 2 mg/ml or higher in a small volume (a few hundred
uL). I was wondering if the protein bound a metal or other prosthetic
group that would give it this color?
you can do a simple wavelength scan at the synchrotron of the protein solution
frozen in a loop.
Best wishes
Kornelius
On Fri, 5 Sep 2008 12:21:29 -0400
Matthew Alan Bratkowski [EMAIL PROTECTED] wrote:
Hello.
I am working with a protein that turns a yellowish-brown color when it is
What value do we expect for the pKa of a protein's C-terminal
carboxylate? pKa values for free amino acids are quite low (2-3),
but it seems to me that this may have something to do with the
proximity of a free amine group; I'd expect a higher value (4-ish?)
for the peptide's C-terminus.
You are correct. See */Protein Sci/* Thurlkill et al. 15 (5): 1214,
http://www.proteinscience.org/cgi/reprint/15/5/1214, which is a recent
effort to codify typical pKa values for protein ionizable groups.
Cheers,
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