Dear Bill,,
On Sun, Oct 26, 2008 at 07:18:05PM -0700, William G. Scott wrote:
I'm actually one of those whom has struggled unsuccessfully to install
Sharp. I can't even remember why, and I am certain it was entirely my
fault, but this did get me wondering...
I guess this is often a more
Dear Joe,
On Mon, Oct 27, 2008 at 06:04:38AM +, joe wrote:
Hi, all:
I have a poor density after phase extension problem.
Usually I use HKL2000 to scale the data and use the macro at the integrate
and scale steps. I got a good native data( P6422 130 130 150 , 90 90 120) at
3.0A
I would like to add my comments that Sharp autoSharp are great
programs that produce excellent phases, and that installation isn't
too hard (even though the whole setup does seem unnecessarily
complicated :-) )
I would also like to say that the Global Phasing people, in particular
Yikes!
Apologies if that was over the top. I guess the chisanbop comment did
not properly convey the hyperbole, and hence humor, I was trying to
achieve. Hopefully my comments can still be taken as the constructive
criticism they were intended to be.
James
On Oct 26, 2008, at 4:57 PM,
Well, in case of installation difficulties, you can always contact
the friendly and helpful staff at [EMAIL PROTECTED] . ;-)
Unfortunately, our friendly and helpful Mac expert is away at the
moment, but I can try to help. If you follow through to the Mac
download page, you have a few choices.
Hello everyone,
We are working on a protein in which a long loop is held in place by a
disulphide bond. All the biochemistry and biophysics of this protein
indicates that the disulphide bond should be intact, but we only see
one end of it in the crystal structure. The protein has not been
Fox GC, Shafiq M, Briggs DC, Knowles PP, Collister M, Didmon MJ,
Makrantoni V, Dickinson RJ, Hanrahan S, Totty N, Stark MJ, Keyse SM,
McDonald NQ.
Abstract
Redox-mediated substrate recognition by Sdp1 defines a new group of
tyrosine phosphatases.
Nature. 2007 May 24;447(7143):487-92. Epub 2007 May
Dear Jacques-Philippe,
Sorry to have been distracted into responding to comments on your
question rather than answering your question itself.
We will shortly be announcing an academic release of a new version of
BUSTER-TNT, which contains several major improvements over the previous
James,
If I may chime in here, as the head of a similar effort half as old and
one quarter the size of Global Phasing...
We scientists heading up private crystallography related software
efforts tend to be hypersensitive to such criticisms because of certain
unfortunate past behaviors of
@all,
we've been comparing various programs during our SGPP times (that was
about 2 years ago) Sharp, Shelx, bp3, mlphare, SnB (BnP). If I
remember it correctly at that time Sharp was the winner, although the
site finding was the major bottleneck/problem. What we ended doing was
finding
OXION is a research initiative grouping 25 UK laboratories based in Oxford,
Cambridge and London and aimed at structural and functional characterisation of
ion channels (http://oxion.dpag.ox.ac.uk/).
Two training fellowships (at the junior or senior postdoctoral level,
application deadline:
Dear All,
I wonder whether it is a good idea to still accept H32 as
a space group in the PDB CRYST1 record, despite it may be used
internally by programs.
* The combination of hexagonal cell parameters and R32 clearly
indicates a hexagonal (obverse) axis setting in the R centered cell.
* The
Tiancen,
While not entirely impossible - this is a formidable task. The answer to
your quesiton depends on the specifics of your situation and on what
additional information you are able to procure. Quite a few kinases have
specific sites; equal or greater numbers are only partially specific
In fact there's another good reason not to use H3 etc when you mean R3:
the H lattice symbol is already in use to mean something completely
different! - namely H-centring in *trigonal hexagonal* (i.e. *not
rhombohedral*) space groups such as P3, P3/1 etc supergroups, so e.g.
H3 is actually a
Good point. Btw, also these nonstandard,
triple ab-plane centered H3x cells can be
readily reindexed standard primitive, so no new
space groups are needed...let's not give the PDB
new ideas...
BR
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Ian
Dear all,
Are there some articles or reviews discussing about the PEG molecules in
crystals or the biochemical features of PEG?
Thanks.
--
Jiamu Du, Ph.D.
State Key Laboratory of Molecular Biology
Institute of Biochemistry and Cell Biology
Shanghai Institutes for Biological Sciences
Chinese
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