Hi Xie,
> Many thanks to all who responded to my earlier query (Drs. Paul
> Swepston, Randy Read and Nicholas Glykos). I am trying to determine the
> structure of a very long coiled coil dimer (roughly 150 residues long)
> by molecular replacement. I don't know if it forms a canonical coiled
With a pseudo translation vector like that the SG could be any of the 8
orthorhombic SGs; P222 P21 22 P21212 P212121 P2 21 2 P2 21 21 P 2 2 21
Test them all, and see if any give a dect solution..
Eleanor
Alison Li wrote:
We recently collected a complete 2.5A MAD dataset. However, finding a
sol
Dear colleagues,
I am having the pleasure to organise a shipment of frozen crystals from
Germany to the UK, and the only way to do this seems to send them by FedEx in a
dry shipper (Taylor & Wharton CP100). My question is, has anybody (perhaps from
Germany?) done this before and give me som
Dear all,
I have a problem with a SAD data set. My crystal is very sensitive to
radiation damage, so I have collected 2 110 frame data sets, 180 deg
apart. I have processed the data with HKL2000 and it turns out that the 2
sets have very different scales. My question is whether it would be
possible
On Monday 02 February 2009, Vesna Serrano wrote:
> Dear all,
> I have a problem with a SAD data set. My crystal is very sensitive to
> radiation damage, so I have collected 2 110 frame data sets, 180 deg
> apart. I have processed the data with HKL2000 and it turns out that the 2
> sets have very di
=
SYNCHROTRON BEAM TIME FOR MACROMOLECULAR CRYSTALLOGRAPHY
AT THE SUPERBENDING MAGNET BEAMLINE X06DA AT SLS
=
Deadline: Sunday, February 15, 2009
Periods:
May 1, 2009
If it is dry (charged with LN2 but empty of free liquid) you can ship it as
non-Dangerous goods. The key words for FedEx (or any air shipping agent) are
"Non-Restricted"
Non-hazardous, non-toxic, and non-flammable are assumed as you are shipping it
non-hazardous. It is useful to have on the shi
ClearSeal film from Hampton works with our UVEX UV microscope, despite some
attenuation of UV light.
For hanging drops, Greiner BioOne's films are the best.
V. Nagarajan
JAN Scientific, Inc.
http://janscientific.com
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.u
Hi,
Can anyone recommend any UV-transmissible (280 nm), adhesive seals
(pre-cut) for 96-well crystallization plates (Greiner, MRC)?
We would like to use them on an crystal imaging system with UV option
(RIGAKU). I've checked seals from Zymark/Calliper Lifescience with a
regular photometer and the
Hi All,
I just downloaded and installed ccp4 in my home directory. The installation
went quite smoothly. For individual ccp4 programs, it seems working ok.
however, when I type ccp4i, it gave a bunch error message. here are the
error message:
Top level CCP4 directory is /home/X/programs/ccp4-6
Hi Uli,
When we send our dry shipper from UK to ESRF, Grenoble, via FedEx we
place a following note on the box:
DRY SHIPPER, Non-hazardous, non-toxic, non-flammable, non-
restricted. Conforms with the IATA regulations Special Provision A800
We write the same in the Contents place of the I
Gees, I go to Washington DC for a couple of days and then a superbowl
party, come back to my stack of emails
and find out I missed out on all the fun... again!
At first glance I thought it looked like a problem with the CCD detector
overloads, but that apparently has been ruled out.
Looks like y
Hi -
With good 2.0 A data (as you seem to have) and a correct solution,
I would be rather surprised if ARP/wARP - REFMAC5 would not refine and
build a model without major trouble.
Did you try that at all ?
To be more detailed I would:
0. Put the mol rep solution in ARP/wARP and let it run (i
On Mon, Feb 02, 2009 at 02:16:38PM -0500, Eric Liu wrote:
> Hi All,
>
> I just downloaded and installed ccp4 in my home directory. The installation
...
> Error in startup script: unknown color name "black"
check your /etc/X11/xorg.conf for a missing/extra line
-> RgbPath "/usr/share/X11/rgb"
Tru
Beam time available @ X6A
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The NIGMS beam line X6A at the National Synchrotron Light Source provides FAST
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an
We are seeking a talented and motivated individual to join the
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The problem was fixed. I changed the original RgbPath in /etc/X11/xorg.conf
from "/usr/X11R6/lib/X11/rgb" to "/usr/share/X11/rgb". I can now run ccp4i.
Thanks everyone for pointing it out.
Eric
On Mon, Feb 2, 2009 at 4:43 PM, Tru Huynh wrote:
> On Mon, Feb 02, 2009 at 04:23:18PM -0500, Eric
A postdoctoral position is available in the lab of Filip Van Petegem at the
University of British Columbia.
Our lab focusses on calcium channels in both the plasma membrane and
intracellular compartments. Calcium is an important 2nd messenger and
channels allowing influx of calcium into the cytop
Hi Tassos,
On Mon, Feb 02, 2009 at 11:13:28AM +0100, Anastassis Perrakis wrote:
> To be more detailed I would:
>
> 0. Put the mol rep solution in ARP/wARP and let it run (in the meantime
> proceed to 1-3 if 0. fails ...)
^^^
You didn't tell us what point 1 is:
> 2. Do some density
Rana Refaey wrote:
Hi,
I was wondering if anyone knows what programme I need to use to subtract the
Fobs of two different crystals from each other.
Regards,
Rana
_
Invite your mail contacts to join your friends list with Windows
On Feb 2, 2009, at 11:39, Clemens Vonrhein wrote:
Hi Tassos,
On Mon, Feb 02, 2009 at 11:13:28AM +0100, Anastassis Perrakis wrote:
To be more detailed I would:
0. Put the mol rep solution in ARP/wARP and let it run (in the
meantime
proceed to 1-3 if 0. fails ...)
^^^
You didn
I am currently trying to refine a structure where a 5 residue stretch of a
chain is in 2 conformations. Oddly enough, 1 of these 5 residues is in dual
conformations in both the conformations! Is there a conventional nomenclature
for defining such dual-dual conformations?
Refmac5 does not accept
I have battled a similar piece of structure in a recent project. There
is no software in crystallography that can handle hierarchical alternative
conformations w/o tricks. I was refining in Shelxl but the same trick
will work elsewhere. I had to define a new residue type, with two heads.
The
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