The combination of electron microscopy and x-ray crystallography for
the structure determination of large biological complexes
18 - 24 October | 2009 |Grenoble| France
The course aims to train junior investigators in both fields (electron
microscopy and X-ray crystallography) to carry out
1) By submitting my structure to the PDB Validation Server for precheck and
validation, will my structure become publicly available in any way?
It will not.
But if you prefer, you can download the validation tools and run them
on your home computer.
However, most recent
.. and ot be honest a structure that passes with flying colors from
the MolProbity or WhatCheck scrutinee,
is unlikely to get stack in PDB submission.
A.
On Jul 7, 2009, at 16:59, Pete Meyer wrote:
1) By submitting my structure to the PDB Validation Server for
precheck and
validation,
Dear Crystallographers,
does anybody know of a simple, fast way to determine whether a protein
sample contains lipid, and roughly to determine the quantity? As corollary,
does anybody know of a quick way to extract/remove the putative lipid? In my
case, I am working with an extremely
Hi Jacob,
there are ways, but simple and fast, I don't know. You can do extraction of
some purified protein with organic solvents and doing TLC. Quantitation may be
harder. In the case of phospholipids you can do a phosphorous determination
(with molybdenum, the protocol should be easy to
Activated charcoal (see serum albumin structure references) can remove
fatty acids.
Good luck-
Steve
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Jacob Keller
Sent: Tuesday, July 07, 2009 1:01 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb]
Also MALDI-TOF can be used to help identify the lipid (with standards).
I vaguely remember one would extract the lipid, from the protein, using
organic solvents then apply that to the MS. When I did it (about 10
years ago) I used non plastic vials and a Hamilton syringe so as not to
contaminate
A post-doctoral associate position is available in the Institute of
Molecular Virology, University of Minnesota to study the cell entry
mechanism of small envelope viruses (Zhang, W., et al 2002. J. Virol.
76:11645-11658; Kielian, M., and F.A. Rey. 2006, Nature Rev. Microbiol.
4:67-76.) using
Meeting Notice:
We invite you to participate in the MX Frontiers at the One Micron Scale
workshop, which will be held at Brookhaven National Laboratory on July 23 and
24, 2009. The workshop will include lectures and discussions about potential
opportunities in macromolecular
Hi all,
I'm trying to refine a structure but it needs six parameter files input in
order to deal with al lthe atoms built in.
minimize.inp keeps crashing with the error : %NBUPDA-ERR: missing nonbonded
Lennard-Jones parameters %%%
I realized after switching up the order of the
Dear Bert,
Your density reminded me a little bit of the paper by Pearson et al.
Biochemistry 39:8575-8584(2000) which describes the water structure in the
catalytic center of urease. I just went back to it and it looks like Figure
3 in that paper may hint some similarities to your case, in that
Apologies for coming late with this comment, but this A280 filter clouding
problem appears to be a common feature of the AKTA machines. The UV unit on
these AKTAs is the same as on the old FPLC machines and the filters are
interchangeable. The filters from the 10-20 year old FPLC machines fit
At 12:01 PM -0500 7/7/09, Jacob Keller wrote:
does anybody know of a simple, fast way to determine whether a
protein sample contains lipid, and roughly to determine the
quantity? As corollary, does anybody know of a quick way to
extract/remove the putative lipid? In my case, I am working with
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