I third it - we used 3.2M + 5% glycerol ammonium sulfate to
cryoprotect crystals grown in 1.2M ammonium suphate (Bayliss et al.
JBC 2002). This really helped diffraction too. My tip when handling
drops at very high salt is to make a little circle of wet tissue paper
around your coverslip
How about a 3-fold disordered metal ion?
Mark
On 19 Aug 2009, at 17:07, Sampath Natarajan wrote:
Dear All,
Currently I'm modeling one structure with 2.6A data. I could find an
unknown density which I located in the interface between the three
similar subunits. This density shows very
Hi Brenda,
Maybe you want to have a look at the cryoprotectant database for
protein crystals.
http://idb.exst.jaxa.jp/db_data/protein/search-e.php
There you can search for your precipitant and get a list of
compatible freezing conditions that worked before including the
reference. It is
Dear All,
I wish to draw your attention to a recent post opening at the Protein
Databank in Europe (http://www.ebi.ac.uk/pdbe/) for a Scientific
Database Curator. Details of the job and application procedure below:
Thanks - Jawahar Swaminathan
PDBe, European Bioinformatics Institute,
Cambridge
Dear Sir,
Thank you all who have replied. I jast want to enquire that if there is any
option in coot to introduce the OH bond with Cys residue as well as to
introduce the secondary peptide like bond between carboxylic carbon with amino
group of lys.
Sincerely
Debajyoti Dutta
On Wed,
NIH-funded postdoctoral position is available immediately in a
membrane protein transport and structural biology laboratory in the
Department of Cell Physiology and Molecular Biophysics at Texas Tech
University Health Sciences Center, Lubbock. The laboratory is well
equipped with the
Dear All,
I wish to bring your attention to a crystallographer postdoctoral opening
at Boston University School of Medicine. Please see the job description below.
Thanks,
HC Guo
Postdoctoral Fellow Position Open
Boston University School of Medicine
Department: Physiology and Biophysics
Hi all-
Thanks to Charles Ballard, who sent me an xplot driver file with
optimization turned off. Once I copied this into my
/usr/local/ccp4-6.1.2/bin folder, sourced and reloaded ccp4i, the plots
opened up without a problem. Please note that, at least in my hands, just
updating to CCP4 version
Posted on behalf of Dr. Jeff Abramson:
A postdoctoral position is available in the laboratory of
Dr. Jeff Abramson at the Department of Physiology, University of
California Los Angeles (UCLA) for a highly motivated individual with a
strong interest in structure and function of
Hi,
Hard to say w/o moving the thing around, but it could be: pep flips, or even
(less likely) wrong chain direction. Incidentally, some of thr the side
chains 'just don't look right to me' - interpret that one as you wish but
e.g. the leucine in the middle seems particularly wrong (try
I agree with Artem. I also don't think you have a register problem.
All of the residues show typical 2fofc density for their types,
especially considering the 3A data. The disappearing act of the asp
carboxyl is typical of solvent exposed aspartates. Assuming the
register is correct, you
11 matches
Mail list logo
