Actually, imosflm works very nicely indeed on windows, and needs no
third-party package. (Unless you consider ccp4 "third-party" as opposed
to essential.)
phx.
On 23/03/2010 19:38, James Holton wrote:
I have been reminded that the front page of ADXV's website does not
have a link to the "wi
Greetings,
We're searching for a 'mushroom' shipping dewar (i.e. rounded sites
and domed lid) to keep FedEx from laying our dry shipper on its side
despite giant arrows and signs stating otherwise.
From my look at descriptions and specifications, I believe the MVE SC
4/3V dry shipper dewa
Hi Regina,
this subject was discussed on PHENIX bulletin board some time ago:
http://www.phenix-online.org/pipermail/phenixbb/2009-December/003074.html
I think this answers your question; please let me know otherwise.
Also, I'm not sure what you mean by "(except that refinement will fail
if t
Hello all.
I have a refinement that includes atoms at special positions, am unsure how to
delineate them in CCP4 vs. PHENIX programs. According to the information, for
CCP4 you can reduce the occupancy by the appropriate amount, depending on the
axis (2-fold to 0.5, 3-fold to 0.33, etc). Howe
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I have been reminded that the front page of ADXV's website does not have
a link to the "windows version" which requires cygwin, but there is a
binary here:
http://www.scripps.edu/~arvai/adxv/adxv_1.9.6/adxv.cygwin.gz
You will need cygwin installed for that to work. Good news is the
latest ver
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Although the extension *.img is used by more than one detector
manufacturer, you are probably looking at data from an ADSC Inc
detector? Andy Arvai's "adxv" is a popular viewer for this format:
http://www.scripps.edu/~arvai/adxv.html
Run it with the option "-nopixmap" if you are using it insi
Dear Tim,
I'd dare to say that SeMet labelling in mammalian cells is in
fact routine (at least in our lab, and others' around the
world) for quite a while :-)
Since you mentioned CHO LecR cells, one can find references as
early as 1994 from Wayne Hendrickson's lab (PMID: 7922031) and
many oth
Dear All,
Thanks to all who gave me good suggestions about my question regarding Buried
surface area and oligomeric interface. I am posting the summary here.
---Biochemical Society Transactions (2008) Volume 36, part 6 (Kobe et al.,)
have a look at this paper. I believe it's exactly wha
Hi
iMosflm will do it for most detector types and is free.
There are other programs out there that would probably work as well (d*Trek,
HKL2000) but I haven't used those so I don't know ;-)
On 23 Mar 2010, at 14:51, Paul Lindblom wrote:
> Hi everybody,
>
> does anybody know a program to disp
Hi everybody,
does anybody know a program to display x-ray (.img) data images in windows?
Thanks,
P.
Hussain Bhukyagps wrote:
i'm trying to solve the structure of protein with ligand.
Can any one help me in uploading ligand library (to fit in the
density) in WinCoot.
This is a good starting point:
http://www.majorgroove.org/questions/49/how-to-add-a-ligand-to-a-model
(Coot depends on C
Hi Tim,
Lactococcus lactis can also produce SeMet proteins, see:
Berntsson et al. Selenomethionine incorporation in proteins expressed in
Lactococcus lactis. Protein Sci (2009) vol. 18 (5) pp. 1121-7
Cheers,
Ronnie
On Mar 23, 2010, at 14:40, Tim Gruene wrote:
> Dear all,
>
> I already receiv
Dear all,
I already received a couple of replies.
* mammalian cells:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.pdf
mentions several mammalian cell lines, including CHO, HEK239, and COS.
Apparently, Lec8 have been used, too.
* Yeast
* stable S2 insect cells
* baculovirus express
--Original Message--
To: CCCP4 Bulletin Board
Subject: Se Met in Yeast
Sent: Mar 23, 2010 1:50 PM
By our collaborators in Rochester (Malkowski,...,Grayhack) established SeMet in
yeast a few years ago.
Sent via BlackBerry by AT&T
Dear Tim,
Although it is not routine, it can be done in mammalian cells too! For example,
have a look at this:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.pdf
HTH,
Luca
Luca Jovine, Ph.D.
Karolinska Inst
Dear all,
since I am currenty preparing a lecture on crystallography I am wondering about
the status quo of the production of SeMet proteins.
In 2003, if I remember correctly, it was possible to express SeMet proteins in
E.coli and insect cells.
Has this been extended to other systems, and if so,
Hi
This is Hussain, and i'm trying to solve the structure of protein with ligand.
Can any one help me in uploading ligand library (to fit in the density) in
WinCoot.
Thank U
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20 percent (v/v).
Steve
> 2,3-BD users,
>
> What sort of concentrations are you using?
>
> Dave
>
> --
> Delivered via an Android.
>
> On Mar 22, 2010 11:45 PM, "Steve Tuske" wrote:
>
> Hi Ulrike,
>
> 2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it
> mostly seems to de
I would not rely on recipes. When I pick a cryo protectant, I always prepare a
set of solutions and take an image from a large loop dipped into solution.
Then I pick the one with the least concentration that does not show ice rings
anymore.
The behaviour can change with a crystal in the loop, but
> ... and on the other side, "laws" like Moore's law seem completely
> descriptive and not at all causal or essential.
http://dx.doi.org/10.1088/0143-0807/16/4/005
;-)
--
Dr Nicholas M. Glykos, Department of Molecular Biology
and Genetics, Democritus University of Thrace, Un
Hello Dave,
I have good experience with concentrations between 10% and 15%
2,3-butanediol. My typical starting concentration is 12.5%.
Good luck.
Eike
Am 23.03.10 08:27 schrieb "David Briggs" unter :
> 2,3-BD users,
>
> What sort of concentrations are you using?
>
> Dave
>
> --
> Delive
2,3-BD users,
What sort of concentrations are you using?
Dave
--
Delivered via an Android.
On Mar 22, 2010 11:45 PM, "Steve Tuske" wrote:
Hi Ulrike,
2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it
mostly seems to decrease mosaicity. I was able to switch from a
thr
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