Hi Lisa,
Why are you so sure there are 4 molecules in the ASU? There may only be
3 and forcing a fourth molecule is causing lots of clashes. In a similar
case, I have seen phaser put two molecules right on top of each other
when I forced it to search for too many molecules.
In your case I
(my last spam)
This is very true. Compared to biomedicine, protein crystallographers are holy
saints: Of 50 landmark papers in oncology, people from Amgen could only
reproduce 6 (11%) and in a similar study, people at Bayer could only reproduce
14 out of 67 (21%) studies. Even more troubling,
Hi,
does anybody know if there is a published list (or does anybody have a
list and is willing to share) with PDB three letter codes for typical
buffer components that show up in crystal structures?
Cheers
Ruth
--
Dr. Ruth Brenk
Biological Chemistry and Drug Discovery
College of Life Sciences
Thank you very much!
Eleanor
On 19 April 2012 19:43, Bret Wallace bretw...@gmail.com wrote:
I noticed this missing when I first installed v. 6.2 as well.
They noted this in the problems page. You just need to replace the
phaser_MR.tcl file with the updated version in the updates page. I
When I hear of a reviewer holding up a publication and then publishing
something similar, my first reaction is fury and I feel the case should be
investigated and this immoral individual should be exposed. However I can
see that there are many shades of gray here. We're all biased in that we
A PhD position funded by a grant is available in the Synchrotron Group
headed by Dr Jean-Luc Ferrer at Institut de Biologie Structurale (IBS
http://www.ibs.fr/spip.php?lang=en) at Grenoble, starting in October
2012. The project will focus on the structural and biochemical
characterizations of
Dear XDS users,
for about 3 weeks a new (non-commercial) version has been available from
http://homes.mpimf-heidelberg.mpg.de/~kabsch/xds/ . It incorporates
improvements, bug fixes, and new features.
If there are any problems/regressions with a new XDS version, let me (or
Wolfgang Kabsch)
Just a thought:
When a reviewer asks for the model/data,
1) The reviewer should be given at most 24-48 hours of time to give
comments after receiving the data.
2) (S)he should declare to the editor that the paper is going to be
accepted if everything with the data/model is okay. The reviewer
Theresa,
For point mutations, we currently use MEGAWHOP, which is a
megaprimer-based whole plasmid PCR method. It has the advantage of using
single mutant primers of modest length (21-24 nt) in combination with
existing flanking primers for the target gene (either the 5' or 3'
flanking
It seems that this discussion has somehow reached the conclusion that if
a reviewer asks for model/data, there absolutely must be an ulterior
motive to cheat you out of your high profile publication.
On the other hand, it seems like the intent of such reviewer is also
misunderstood as if the only
Would the self rotation map make sense with 4 molecules ?
Jürgen
Sent from my iPad
On Apr 20, 2012, at 2:55,
herman.schreu...@sanofi.commailto:herman.schreu...@sanofi.com
herman.schreu...@sanofi.commailto:herman.schreu...@sanofi.com wrote:
Hi Lisa,
Why are you so sure there are 4 molecules
P21.. You sure about this space group? (very high confidences for space group
and laue group in pointless?)
F
On Apr 19, 2012, at 12:20 AM, LISA wrote:
Hi all,
I am trying to solve one structure by molecular replacement with phaser in
CCP4. This a complex of a multi-domain domains
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