Hi,
We ran Scala program with 2 A resolution cut-off and it gave mean I / sig I
value as follows
Overall: 7.3 OuterShell: 2.0 InnerShell: 16.5
CC(1/2) : Overall: 0.994 OuterShell: 0.665 InnerShell: 0.997
We also tried Scala with 2.33 A resolution cut-off and the statistics are
as
Hi,
We checked all possible space groups of orthorhombic crystal system using
Scala and Pointless but the statistics show that P212121 is the possible
space group.
Thank You,
Satya Dev
On Fri, Mar 17, 2017 at 8:03 PM, Teplyakov, Alexey [JRDUS] <
atepl...@its.jnj.com> wrote:
> Check the space
The “name” means atom names, e.g. n for nitrogen (backbone), yes, you would use
“name” (or n. in short) in your command.
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Alex Lee
Sent: Friday, March 17, 2017 2:34 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [EXTERNAL] [ccp4bb]
Dear CCP4BB members,
I tried to use pymol command to align two proteins, I read the pymol wiki
and I could not understand the command grammars (I am not computer major
and not familiar with machine language).
For example pymol wiki says as below:
Furthermore, you may wish to restrict the
I recall a case of this a few years ago where it had to do with the
relative concentration of the protein to the buffer/salt molecules (can't
remember which anymore), which ended up being important in the binding that
was observed. So it was entirely a concentration effect that caused the
Le Vendredi 17 Mars 2017 16:07 CET, Nicholas Larsen
a écrit:
Do you mean that the affinity from ITC is 100-fold weaker ? Which would mean
that the Kd values from ITC are 100-fold larger (in the range 0.1-1 µM) ?
If this is the case, it makes me think to a
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Dear colleagues,
We have a target where people have measured Kd's for ligands using
radioligand binding assays. Several publications report Kd's of single
digit nanomolar and we are able to reproduce that data using this assay
format. When we try to do the same measurement using ITC, we generate
Dear All,
In Coot, I could adjust the densities of map (2Fo-Fc in my case) radius,
but I'd like to show the density on selective residues, not on the
unselected part of structure, is there a way to do it? I am using WinCoot
0.81.
In addition, could Pymol do it?
Thanks ahead!
One way is to refine the best chain out of four, first and then generate a
seperate pdb for that chain only and use molecular replacement to find the
rest of the three chains. It does help with improving map to certain
extent. Also delete the missing residues one by one and then run refmac to
see
Dear all,
I solved a structure at 2.0 A resolution with R-work and R-free values 0.25
and 0.32 respectively and I am stuck at Refmac step where there is no
further reduction in R-factor.
The above stated values were obtained after several rounds of manual
refinement followed by refmac. There are
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