Dear colleagues,
We are pleased to announce the 8th South American Macromolecular
Crystallography School 2021 "Structural Biology to enhance high impact research
in health and disease” to be held from Sep 20 to Oct 1, 2021
Please find the application form and further information at
Indeed, and its applications (see below) are, umm, quite interesting, both in
commercial/industrial food processing and “molecular gastronomy” [great phrase
there, eh?]
https://modernistpantry.com/products/activa-rm-transglutaminase.html
-MW
From: CCP4 bulletin board on behalf of Patrick
Transglutaminase, also known in some circles as “meat glue."
> On 17 Jun 2021, at 11:50 AM, Bryan Lepore wrote:
>
> Greetings
>
> This enzyme meets none of the stipulations, but I will point out as it is
> somewhat unusual to find in a grocery store :
>
> A food product called Just Egg
Greetings
This enzyme meets none of the stipulations, but I will point out as it is
somewhat unusual to find in a grocery store :
A food product called Just Egg contains “transglutaminase” as an ingredient.
Make of that what you will.
-Bryan W. Lepore
(No affiliation with Just Egg or anything
All the previously suggested proteins are awesome candidates!
Here are a few more in case you want to add some extra 'zing' to your
curriculum:
- luciferase
- magneto reactive protein(s) that can be isolated with strong magnets
- spy-tag and spycatcher (covalently reactive proteins!)
- split GFP
We developed a senior undergraduate biochemistry lab around an acid phosphatase
from Francisella tularensis (FtHAP).
https://pubmed.ncbi.nlm.nih.gov/27980518/
The enzyme is easy to purify and crystallize, and the crystals diffract well.
L-tartrate and phosphate ion are inexpensive inhibitors,
I know not many will have met Jenny in person but maybe more have read
Crystal Structure Analysis - a Primer by Glusker and Trueblood.
It is an excellent book, and widely plagiarized!
Please send a message if you would like to
Eleanor Dodson
--
*From:* Miriam Rossi
GFP:H6 is a nice place to start. it doesn't have enzymatic activity, but it
expresses nicely in coli and is visible to the human eye during
affinity-purification on NiNTA/imidazole (and the fluorescence is interesting
too). the biochemistry dept. here uses it routinely with undergrads.
cheers
We can recommend a small flavoprotein. which we have used for different
biochemistry courses. It is easy to express and purify with IEX snd GF (no
tag), and in the 15 years we have used it, students always get crystals.
We have published it in Biochemistry and molecular biology education:
"A
Dear Jiang
If you gave Mosflm the “correct” space group, then you can skip the Pointless
step, but I don’t think the pipelines in ccp4i and certainly ccp4i2 let you do
that. However, it shouldn’t hurt.
I don’t think you can skip the merging step in Aimless, and anyway it is that
step which
10 matches
Mail list logo
