Re: [ccp4bb] the f' and f'' of heavy cluster

he cluster and get better phase information that way. Jason. -- Jason Busby PhD Student Laboratory of Structural Biology School of Biological Sciences University of Auckland Thomas Building 110 3a Symonds St Private Bag 92019 Auckland 1142 New Zealand ph: +64 9 3737599 ext 84155 fx: +64 9 3737

Re: [ccp4bb] experimental phasing + MR

://dx.doi.org/10.1107/S0907444909029643 And an Auto-Rickshaw server: http://www.embl-hamburg.de/Auto-Rickshaw/ Cheers, Jason. — Dr Jason Busby Laboratory of Structural Biology School of Biological Sciences The University of Auckland Private Bag 92019 Auckland Mail Centre Auckland New Zealand ph

Re: [ccp4bb] Poor experimental phases for a largish structure...

around again. Jason. — Dr Jason Busby Laboratory of Structural Biology School of Biological Sciences The University of Auckland Private Bag 92019 Auckland Mail Centre Auckland New Zealand ph: +64 9 3737599 ext 88958 On 19/02/2015, at 1:51 am, William Chao mailto:william.c...@cancer.org.uk

Re: [ccp4bb] paired refinement

and see if including the extra data to 2.6 has made your structure better. Usually this means either Rfree goes down, or Rfree stays the same but Rwork goes up (indicating less overfitting). Cheers, Jason. -- Dr Jason Busby Laboratory of Structural Biology School of Biological Sciences The

[ccp4bb] Difficulty indexing diffraction, cell too small

hanks, Jason. -- Jason Busby PhD Student Laboratory of Structural Biology School of Biological Sciences University of Auckland Thomas Building 110 3a Symonds St Private Bag 92019 Auckland 1142 New Zealand ph: +64 9 3737599 ext 83888 fx: +64 9 3737414

Re: [ccp4bb] Difficulty indexing diffraction, cell too small

sending some on a synchrotron trip. I think I will also sacrifice a crystal or two to dissolve and check that I have crystallised the protein I'm working with. Cheers, Jason. -- Jason Busby PhD Student Laboratory of Structural Biology School of Biological Sciences University of Auckland T

[ccp4bb] Recompile fsearch with increased memory

memory available to fsearch? I have tried increasing MAX3D in the fsearch source, but could not get it to link on my system (ld: symbol(s) not found). I'm running ccp4 6.2.0 on Mac OS X 10.6.8 Any help would be appreciated. Thanks, Jason. -- Jason Busby PhD Student Laboratory of Struc

Re: [ccp4bb] Recompile fsearch with increased memory

Ah thanks, I've managed to get it sorted out. In case anyone else has this issue, I downloaded the CCP4 source code, increased MAX3D in fsearch.f, and recompiled. I first built CCP4 (./configure && make) and then fsearch explicitly (cd src && make fsearch). Thanks, Jason

[ccp4bb] Large unit cell, overlaps

ution. You can see a zoomed-in part of FRAME.CBF here: http://imgur.com/1WShV Should I be concerned with this? The crystal mosaicity from XDS is 0.25, so fairly low. What can I do about this, should I try smaller oscillation angles? Thanks, Jason. -- Jason Busby PhD Student Laboratory of Str

Re: [ccp4bb] co-express two proteins in E.coli

I've had success with pet-Duet. http://ecoliwiki.net/colipedia/index.php/pETDuet-1 Jason. -- Jason Busby PhD Student Laboratory of Structural Biology School of Biological Sciences University of Auckland Thomas Building 110 3a Symonds St Private Bag 92019 Auckland 1142 New Zealand ph:

Re: [ccp4bb] Large unit cell, overlaps

22121 or P212121. My XDS.INP has SPOT_RANGE commented out so I believe the default is to use all the data for indexing. Cheers, Jason. -- Jason Busby PhD Student Laboratory of Structural Biology School of Biological Sciences University of Auckland Thomas Building 110 3a Symonds St Private B

Re: [ccp4bb] Large unit cell, overlaps

large heterodimer, and this unit cell would put 2 in the ASU and a solvent content of 56%, so this seems reasonable. Mosflm also picks the same unit cell, and the predictions seem to match up with spots (although mosflm predicts lots of overlaps) Cheers, Jason. -- Jason Busby PhD Student

Re: [ccp4bb] Large unit cell, overlaps

slices or try and position the crystal in the loop at a different angle, or what. Thanks for the help, Jason. -- Jason Busby PhD Student Laboratory of Structural Biology School of Biological Sciences University of Auckland Thomas Building 110 3a Symonds St Private Bag 92019 Auckland 1142 New Z

Re: [ccp4bb] Coot 0.7 Saving Dialog opens behind main window

Another work-around is to use the command-tilde (⌘ + ~) keystroke. That will cycle through all the windows of the current program. Jason. -- Jason Busby PhD Student Laboratory of Structural Biology School of Biological Sciences University of Auckland Thomas Building 110 3a Symonds St Private

Re: [ccp4bb] Stable Refinement as Low(ish) resolution

scaling parameters etc. In CCP4i it is “Run Low Resolution Refinement” on the refinement page. I don’t think it is in CCP4i2 yet. Jason. -- Dr Jason Busby Laboratory of Structural Biology School of Biological Sciences The University of Auckland Thomas Building 110 3A Symonds St Private Bag

Re: [ccp4bb] coot install problems under Mac OS X 11 (El Capitan)

it around as normal. Jason. -- Dr Jason Busby Laboratory of Structural Biology School of Biological Sciences The University of Auckland Thomas Building 110 3A Symonds St Private Bag 92019 Auckland Mail Centre Auckland New Zealand ph: +64 9 3737599 ext 83888 On 24/09/2017, at 11:57 AM, William