it around as normal.
Jason.
--
Dr Jason Busby
Laboratory of Structural Biology
School of Biological Sciences
The University of Auckland
Thomas Building 110
3A Symonds St
Private Bag 92019
Auckland Mail Centre
Auckland
New Zealand
ph: +64 9 3737599 ext 83888
On 24/09/2017, at 11:57 AM, William
scaling parameters
etc.
In CCP4i it is “Run Low Resolution Refinement” on the refinement page. I don’t
think it is in CCP4i2 yet.
Jason.
--
Dr Jason Busby
Laboratory of Structural Biology
School of Biological Sciences
The University of Auckland
Thomas Building 110
3A Symonds St
Private Bag
and see if including the extra data
to 2.6 has made your structure better. Usually this means either Rfree goes
down, or Rfree stays the same but Rwork goes up (indicating less overfitting).
Cheers,
Jason.
--
Dr Jason Busby
Laboratory of Structural Biology
School of Biological Sciences
The
around again.
Jason.
—
Dr Jason Busby
Laboratory of Structural Biology
School of Biological Sciences
The University of Auckland
Private Bag 92019
Auckland Mail Centre
Auckland
New Zealand
ph: +64 9 3737599 ext 88958
On 19/02/2015, at 1:51 am, William Chao
mailto:william.c...@cancer.org.uk
://dx.doi.org/10.1107/S0907444909029643
And an Auto-Rickshaw server: http://www.embl-hamburg.de/Auto-Rickshaw/
Cheers,
Jason.
—
Dr Jason Busby
Laboratory of Structural Biology
School of Biological Sciences
The University of Auckland
Private Bag 92019
Auckland Mail Centre
Auckland
New Zealand
ph
he cluster and get better
phase information that way.
Jason.
--
Jason Busby
PhD Student
Laboratory of Structural Biology
School of Biological Sciences
University of Auckland
Thomas Building 110
3a Symonds St
Private Bag 92019
Auckland 1142
New Zealand
ph: +64 9 3737599 ext 84155
fx: +64 9 3737
Another work-around is to use the command-tilde (⌘ + ~) keystroke. That will
cycle through all the windows of the current program.
Jason.
--
Jason Busby
PhD Student
Laboratory of Structural Biology
School of Biological Sciences
University of Auckland
Thomas Building 110
3a Symonds St
Private
slices or try and position
the crystal in the loop at a different angle, or what.
Thanks for the help,
Jason.
--
Jason Busby
PhD Student
Laboratory of Structural Biology
School of Biological Sciences
University of Auckland
Thomas Building 110
3a Symonds St
Private Bag 92019
Auckland 1142
New Z
large heterodimer, and
this unit cell would put 2 in the ASU and a solvent content of 56%, so this
seems reasonable.
Mosflm also picks the same unit cell, and the predictions seem to match up with
spots (although mosflm predicts lots of overlaps)
Cheers,
Jason.
--
Jason Busby
PhD Student
22121 or P212121.
My XDS.INP has SPOT_RANGE commented out so I believe the default is to use all
the data for indexing.
Cheers,
Jason.
--
Jason Busby
PhD Student
Laboratory of Structural Biology
School of Biological Sciences
University of Auckland
Thomas Building 110
3a Symonds St
Private B
I've had success with pet-Duet.
http://ecoliwiki.net/colipedia/index.php/pETDuet-1
Jason.
--
Jason Busby
PhD Student
Laboratory of Structural Biology
School of Biological Sciences
University of Auckland
Thomas Building 110
3a Symonds St
Private Bag 92019
Auckland 1142
New Zealand
ph:
ution. You can see a zoomed-in part of FRAME.CBF
here:
http://imgur.com/1WShV
Should I be concerned with this? The crystal mosaicity from XDS is 0.25, so
fairly low. What can I do about this, should I try smaller oscillation angles?
Thanks,
Jason.
--
Jason Busby
PhD Student
Laboratory of Str
Ah thanks, I've managed to get it sorted out.
In case anyone else has this issue, I downloaded the CCP4 source code,
increased MAX3D in fsearch.f, and recompiled. I first built CCP4 (./configure
&& make) and then fsearch explicitly (cd src && make fsearch).
Thanks,
Jason
memory available to fsearch? I have
tried increasing MAX3D in the fsearch source, but could not get it to link on
my system (ld: symbol(s) not found).
I'm running ccp4 6.2.0 on Mac OS X 10.6.8
Any help would be appreciated.
Thanks,
Jason.
--
Jason Busby
PhD Student
Laboratory of Struc
sending some on a
synchrotron trip.
I think I will also sacrifice a crystal or two to dissolve and check that I
have crystallised the protein I'm working with.
Cheers,
Jason.
--
Jason Busby
PhD Student
Laboratory of Structural Biology
School of Biological Sciences
University of Auckland
T
hanks,
Jason.
--
Jason Busby
PhD Student
Laboratory of Structural Biology
School of Biological Sciences
University of Auckland
Thomas Building 110
3a Symonds St
Private Bag 92019
Auckland 1142
New Zealand
ph: +64 9 3737599 ext 83888
fx: +64 9 3737414
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