Hi,
There are tons of these listed on eBay, many in very good condition or even
unused. I used to get them there all the time.
Best
On Tue, Apr 28, 2020 at 8:51 AM Artem Evdokimov
wrote:
> CCP4 friends,
>
> Sorry for the somewhat off-topic post!
>
> For many years I've been a fan of the
I once had a problem that seemed to manifest itself similarly to what you
describe. It turned out to be a misindexing of the diffraction origin
by +/- 1 due to a slightly incorrect beam center. You can use the pointless
"centre" option to do a search for the correct center. There's an example
of
Hi,
Long crystallization times and irreproducibility together with missing
residues points strongly to some slow proteolysis in your crystal drops
that eventually generated a crystallizable fragment. A lot of people
actually do this on purpose by adding proteases to generate stable
fragments.
to experimentally determine accurate
extinction coefficients is the Edelhoch method. You can read about it here:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2143013/pdf/8563639.pdf
-Tom
---
Thomas Cleveland
NIST Center for Neutron Research
Gaithersburg, MD
On Sun, Aug 20, 2017
amounts of time in one case, where I no longer had
to concentrate/dialyze liters worth of media but could just filter it and
shoot it through a column.
-Tom
-
Thomas Cleveland, Ph.D.
NIST Center for Neutron Research
100 Bureau Dr. MS 6102
Gaithersburg, MD 20899-6102
removing it.
Thanks,
Thomas Cleveland
:09 AM, Thomas Cleveland wrote:
Hi all,
I'm trying to put together some homemade syringe couplers following
the published instructions from the Caffrey group. I'm having a bit
of trouble with this part:
The stainless steel ferrule of the second needle is removed and
placed
Hi everyone,
Thanks for all your input. I will consider processing and depositing
the data out to 2.35 A, but refining only against data up to 2.55 A.
Just to give a little more information, and make sure what I'm doing
is reasonable, I am attaching below the table of statistics vs.
resolution
Hi all,
This is a basic question and I'm sure the answer is widely known, but
I'm having trouble finding it.
I'm working on my first structure. I have a dataset that I processed
in XDS with a resolution cutoff of 2.35 A, although the data are
extremely weak-to-nonexistent at that resolution
could drag the lower right corner to make the window *smaller*,
but it would not let me make it any bigger.
Reginald, I'll take a look at that.
Thanks,
Thomas
On Mon, Jun 24, 2013 at 10:46 PM, Thomas Cleveland
thomas.clevel...@gmail.com wrote:
Has anyone else encountered this? When I go
ttf-xfree86-nonfree-syriac
xfonts-75dpi
xfonts-100dpi
xfs
xfstt
(ttf-mscorefonts-installer was already installed by default)
Everything is working fine now. Thanks again.
-Thomas
On Tue, Jun 25, 2013 at 10:23 AM, Thomas Cleveland
thomas.clevel...@gmail.com wrote:
Hi everyone,
Thanks
://www.dropbox.com/s/muwblcgohhxu94c/iMosflm-cut-off.png
Thanks,
Thomas Cleveland
Hi,
I agree with others here. We produce virus in homemade ISFM and supplement
with 2% serum, then store at 4 C protected from light. We don't titer. I
have noticed no difference in expression over 1-2 years.
-Tom Cleveland
On Sep 27, 2012 8:19 PM, Vitali Stanevich stanev...@wisc.edu wrote:
Jerry,
In my experience with GE's HiTrap Chelating HP columns (which use the
tridentate IDA linkage, rather than the tetradentate NTA), capture is
usually quite good as long as the protein is well behaved and the tag
isn't occluded. It is definitely possible to capture proteins at the
level of
Hi,
I have been extremely happy with the latest Ubuntu release, on both a
Toshiba tablet (the touch screen worked right out of the box) and on a
desktop with the proprietary NVIDIA driver. I haven't had to update,
so I don't know what happens with the NVIDIA driver in that case, but
I can tell
10 L prep of liquid media. Note that
these prices include institutional discounts for some components that
may or may not apply to you.
I can send you our protocols if you are interested.
-Tom
---
Thomas Cleveland
Leahy Lab
Biophysics Biophysical
Rongjin,
It does look like you have the same problem I did. I was never totally able
to figure it out completely. First of all, it was only a problem with some
PDB files and not others. Modifying generic_objects.py (i.e., change the
line if (reduce_status): to if (True): ) as suggested by Paul
Bernhard,
I have tried this using several versions of coot. I tried it first in
the coot that came packaged with ccp4 (Coot 0.5.2). I then tried
WinCoot-0.6-pre-1-revision-2411, which is one of the later revisions
of 0.6-pre, just to see if a more recent version would work. As far
as I can
.
Thanks again,
Tom
On Mon, Oct 12, 2009 at 1:54 PM, Paul Emsley paul.ems...@bioch.ox.ac.uk wrote:
Thomas Cleveland wrote:
Bernhard,
I have tried this using several versions of coot. I tried it first in
the coot that came packaged with ccp4 (Coot 0.5.2). I then tried
WinCoot-0.6-pre-1
hydrogens).
However, at the very end, I get the message BL WARNING:: reduce didnt run
ok, so stop here! It seems like probe never runs.
Does anyone know how to solve this?
Thanks,
Thomas Cleveland
(graduate student, Johns Hopkins University)
20 matches
Mail list logo
