[ccp4bb] Postdoctoral Position - Ohio State University

Dear All, Applications are invited for a joint postdoctoral scientist position in the laboratories of Dr. Sarah Heissler and Dr. Krishna Chinthalapudi at the Ohio State University. The research project focuses on understanding the role of myosin motors and other cytoskeletal proteins in health

[ccp4bb] Postdoctoral position in structural biology

Dear All, Applications are invited for a joint postdoctoral position in the laboratories of Dr. Sarah Heissler and Dr. Krishna Chinthalapudi at the Ohio State University. The research project focuses on understanding the role of myosin motors and other cytoskeletal proteins in health and

[ccp4bb] postdoctoral Fellow Position

Dear All, We have an immediate opening for a talented, highly motivated postdoctoral fellow in my laboratory at the Ohio State University located in Columbus, Ohio. The research in our laboratory is mainly focused on understanding the role of cytoskeletal proteins in cell adhesion dynamics. We

Re: [ccp4bb] changes in small sections of secondary structure

simulation times if at all if your protein is dynamic. Hope this helps! Krish On Fri, Oct 18, 2013 at 2:52 PM, Mahesh Lingaraju mxl1...@psu.edu wrote: Hello experts Attached is an image showing different crystal structures of the same protein in diffrent states (mutant vs substrate bound vs

Re: [ccp4bb] Membrane Protein expression and purification

something out of it! Thank you very much for all your suggestions. Krish On Fri, Jul 12, 2013 at 2:34 PM, Timothy Craig tlmcra...@hotmail.comwrote: Hi Krish, You may need to do some construct engineering to get your protein to express well to the plasma membrane. These steps involve creating

Re: [ccp4bb] Membrane Protein expression and purification

Hi John, Thank you very much for all the valuable suggestions and the helpful link. I think it's worth trying S.cerevisiae both for expression and also protecting the environment from that strong Pichia odour. Krish On Fri, Jul 12, 2013 at 3:17 PM, John Lee kw...@msg.ucsf.edu wrote: Hi

[ccp4bb] Membrane Protein expression and purification

Dear All, First of all sorry for bringing the non-ccp4 post. As our CCP4 community is filled with experts in various fields of structural biology: I'd like to get some help/suggestions from the membrane proteins expert community. I'm trying to express my membrane protein in Pichia pastoris

Re: [ccp4bb] Out of topic: Plot Ligand-protein interactions

Dear Wenhe, You don't need any skills to use Ligplot. Only thing you have to give is the residue number of the ligand or the small molecule you are interested in the command line. There are other commercial software but i don't know whether they are of any interest to you ! MOE or MAESTRO

Re: [ccp4bb] Off Topic: Isothermal Titration Calorimetry (ITC)

I second Stephen's comments on NanoITC. I haven't seen any improvement in terms of volume. The total volume one should use is very much comparable to MicroCal VP-ITC. Analysis software is OK--not so good--crashing some times--GUI needs to bit robust in handling the data. HTH ! Krishna

Re: [ccp4bb] unusual bond lengths in PRODRG cif file

Dear Shveta, I have seen similar trends with PRODRG. I recommend you to use other molecular modeling programs like Hyperchem or Maestro to get more consistent geometric parameters for small molecules. Once you know the bondlengths and bond angles from aforesaid molecular modeling programs, you

Re: [ccp4bb] stoichiometry of complex and incubator

Dear Min, You can use microscale thermophoresis for this kind of tricky cases when you have limited amount of protein and ligands. There is enough literature on this topic. have a look in the following reference: Proc Natl Acad Sci U S A. 2006 Dec 26;103(52):19678-82 Hope this helps. Regards,

Re: [ccp4bb] refinement problem

Hi, It would be nice if you mention more clearly how you have done TLS..i.e.,domains or individual residues or molecules !! You should keep in mind that you have 2.8A data. I would do TLSANL with domains or molecules. Best regards, Krishna Ch PhD Student Hannover Medical school Germany On