[ccp4bb] Cryo-EM Facility Manager, Montana State University

Dear Colleagues, Montana State University (Bozeman Montana) seeks a manager for our cryo-TEM Core Facility (https://www.montana.edu/cryo-electron-microscopy/). The facility currently houses a 200 kV Talos Arctica cryo-TEM equipped with a Gatan K3 direct electron detector, a cryo-capable 120

[ccp4bb] Cryo-EM Grant Program: Deadline March 31st 2024

Dear Community, You're invited to apply now to the Thermo Scientific Cryo-EM Grant Program and tell us how you plan to use cryo-EM methods to accelerate and advance your

[ccp4bb] Cryo-EM specialist position at UC Irvine

The University of California, Irvine is currently accepting applications for a specialist position to manage the day-to-day operations of electron microscopy facilities and services to users at the UC Irvine Materials Research Institute (IMRI). IMRI operates a large number of open-access user

[ccp4bb] Cryo-EM Postdoctoral Scholar positions @ Penn State

Membrane Structural Biophysics at The Pennsylvania State University The laboratory of Dr. Kenneth Lee in the Department of Cellular and Molecular Physiology at the Pennsylvania State University College of Medicine invites applications for TWO new NIH-funded postdoctoral positions to study

[ccp4bb] cryo-electron tomography experiments at the ESRF CM01 cryo-EM facility

Dear Colleagues, the ESRF, Grenoble is opening a new cryo EM service to include cryo-electron tomography experiments at its cryo-EM facility, CM01. In CM01, both cellular and macromolecular cryo-electron tomography will be performed for users’ samples. Applications can be made through the

[ccp4bb] Cryo-EM STRUCTURAL BIOLOGY POSTDOCTORAL RESEARCH ASSOCIATE position (University of Liverpool, UK)

UNIVERSITY OF LIVERPOOL DEPARTMENT OF BIOCHEMISTRY AND SYSTEMS BIOLOGY Cryo-EM STRUCTURAL BIOLOGY POSTDOCTORAL RESEARCH ASSOCIATE £38,250 - £40,521 pa An exciting opportunity has emerged to join the Molecular Biophysics Group to work on a BBSRC-supported

[ccp4bb] Cryo-EM specialist position at CBI / IGBMC, Strasbourg

Dear all, here is an announcement for a cryo-EM specialist position at the Centre for Integrative Biology, Strasbourg, France, to join an exciting structural biology environment with many challenging projects. Please see below for a description. This is also an opportunity to check whether

[ccp4bb] Cryo- EM postdoctoral position in Glasgow

A post-doc position in cryo-EM is available in Glasgow (see below). Please address all inquiries to paula.dafons...@glasgow.ac.uk . Cheers, Mads Dear all, I'm looking for enthusiastic candidates for a postdoctoral position in my lab at the

[ccp4bb] Cryo-EM Scientist position

*Scientist I Biochemistry (cryo-EM) – Center for Protein Degradation * *Overview*Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world-renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of

[ccp4bb] Cryo-EM Researcher positions @ Penn State

Membrane Structural Biophysics at The Pennsylvania State University The laboratory of Dr. Kenneth Lee in the Department of Cellular and Molecular Physiology at the Pennsylvania State University College of Medicine invites applications for new NIH-funded postdoctoral

[ccp4bb] Cryo-EM Core Director position, University of Nebraska-Lincoln

Dear Structural Biologists, The University of Nebraska is hiring a Core Director (Research Assistant Professor) for our Glacios TEM with a Selectris energy filter, Falcon 4i detector, and micro-ED capability (delivery expected 12/2022). This hire is an addition to a tenure-leading hire in

[ccp4bb] cryo-EM/cryo-ET access at CBI via iNEXT-Discovery

Dear all, this is to inform interested users from academics and industry that the Centre for Integrative Biology (CBI) / IGBMC at Illkirch/Strassburg, France is happy to offer - as part of the iNEXT-Discovery infrastructure - 3 types of access modalities: - high-resolution single particle

[ccp4bb] cryo-ET postdoc position in mitochondrial genome maintenance - Center for Protein Research at the University of Copenhagen (Kummer)

Final reminder: The application deadline for our postdoc position in in situ cryoEM of mitochondrial genome maintenance is approaching on 31 January 2022! My group is looking for an excellent and highly motivated postdoc to investigate mitochondrial dynamics and their correlation with

[ccp4bb] cryo-ET postdoc position in mitochondrial genome maintenance - Center for Protein Research at the University of Copenhagen

Gentle reminder that the application deadline for our postdoc position in in situ cryoEM of mitochondrial genome maintenance is approaching on 31 January 2022! My group is looking for an excellent and highly motivated postdoc to investigate mitochondrial dynamics and their correlation with

[ccp4bb] cryo-ET postdoc position in mitochondrial genome maintenance - Center for Protein Research at the University of Copenhagen

The newly established research group of Eva Kummer is looking for an excellent and highly motivated postdoc to investigate mitochondrial dynamics and their correlation with maintenance and expression of mitochondrial DNA. We look for someone to drive establishment of in situ imaging workflows

[ccp4bb] Cryo-EM Center Director Opportunity

Dear CCP4, An opportunity is available for a Cryo-EM Center Director Position at the Hauptman-Woodward Medical Research Institute. The Institute is equipped with a Thermo Fisher Glacios 200 kV system with Falcon IV detector. A CETA-D detector and a dedicated computing cluster are also

[ccp4bb] Cryo-EM Researcher positions @ Penn State

Membrane Structural Biophysics at The Pennsylvania State University College of Medicine The laboratory of Dr. Kenneth Lee invites applications for a postdoctoral position and a research technician position. We take a multi-disciplinary structural biology approach to study the mechanism of

[ccp4bb] Cryo-EM Webinar: Keeping up with the chemists with cloud computing

In a few hours we present a webinar: Keeping up with the chemists - Cloud processing for pharma cryo-EM with speakers from AstraZenica, Amazon and Thermo Fisher Scientific. * Discuss the impact of fast reconstructions for drug design * Explain how cloud computing brings scalability to cryo-EM

[ccp4bb] Cryo-EM Facility Manager - Berkeley California

Dear Colleagues, I would like to draw your attention to a job opening here in Berkeley California, for a Cryo-EM Facility Manager: https://lbl.referrals.selectminds.com/jobs/cryo-em-facility-manager-3801 This position requires expertise in biological cryo electron microscopy

[ccp4bb] Cryo-EM data processing specialist at the Hauptman-Woodward Medical Research Institute

To anyone who may be interested, please see the application instructions below. The search is open but will close soon. The Cryo-EM Center at the Hauptman-Woodward Medical Research Institute (HWI) seeks a scientist to enrich its scientific activities. The newly-built Center is purposefully

[ccp4bb] Cryo-ET Faculty recruitment at CWRU

Dear All, I wanted to bring to your attention that the Department of Neurosciences at Case Western Reserve University has an open search for tenure-track faculty with expertise in CryoET and cellular neuroscience. CWRU is now in the process of major expansion of infrastructure for cryo-EM and

[ccp4bb] Cryo-EM facility Manager University of Edinburgh

Dear All, The University of Edinburgh is looking for a cryo-EM facility manager to maintain and run our cryo-transmission electron microscopy facility (http://cryo-em.bio.ed.ac.uk/). This is a great opportunity to join a vibrant institute with an excellent

[ccp4bb] cryo-EM postdoc position in Klaholz lab CBI / IGBMC, Strasbourg

Dear all, for those still interested in experimentally determined structures (maybe to complement predictions? ;-) we have a post-doctoral researcher position to join the Klaholz group at the Centre for Integrative Biology, IGBMC, Illkirch, France:

[ccp4bb] Cryo-EM access at LISCB

Dear colleagues The Midlands Regional Cryo-EM Facility at LISCB houses a TFS Titan Krios equipped with a Gatan BioQuantum/K3. Applications for microscope data collection or screening time from academia and industry are welcome with more information on modes of access and pricing available on

[ccp4bb] Cryo-EM Validation in the Age of SARS-CoV-2: Methods, Tools and Applications. 18 - 20 November 2020.

Dear CCP4-ers, We are pleased to announce a three day online symposium on "Cryo-EM Validation in the Age of SARS-CoV-2: Methods, Tools and Applications" which will take place 18, 19 & 20 November 2020. The meeting has been organised by the UK EM Validation Network to present current

[ccp4bb] Cryo-EM beamline scientist permanent position at CBI / IGBMC, Strasbourg

Dear all, here is an announcement for a permanent position as cryo-EM beamline scientist at the Centre for Integrative Biology, Strasbourg, France. Deadline is tomorrow 2.7.2020 12 am CET. With best regards, Bruno Klaholz Cryo-EM beamline scientist (permanent position): The IGBMC/CBI is

[ccp4bb] Canceled: [ccp4bb] Cryo-EM for Drug Discovery | Virtual event | June 16, 2020

RTICIPANT;PARTSTAT=NEEDS-ACTION;RSVP=TRUE;CN=Robert Kee nan:MAILTO:bkee...@uchicago.edu ATTENDEE;ROLE=OPT-PARTICIPANT;PARTSTAT=NEEDS-ACTION;RSVP=TRUE;CN=nkuwabara0 3...@gmail.com:MAILTO:nkuwabara0...@gmail.com DESCRIPTION;LANGUAGE=en-US:\n UID:04008200E00074C5B7101A82E0089A6A77619C3DD6010

[ccp4bb] Cryo-EM for Drug Discovery | Virtual event | June 16, 2020

featuring the latest solution to address current challenges wi th SBDD. UID:04008200E00074C5B7101A82E0089A6A77619C3DD601000 01000FA9E141292FFB14EBE217E5DF662FCF9 SUMMARY;LANGUAGE=en-US:[ccp4bb] Cryo-EM for Drug Discovery | Virtual event | June 16\, 2020 DTSTART;TZID

[ccp4bb] Cryo-EM for Drug Discovery | Virtual event | June 16, 2020

Cryo-EM for Drug Discovery | June 16, 2020 What if you could get structural insights at near-atomic resolution of your most challenging drug targets at a pace that meets that of your drug discovery projects? >From around the world you are invited to join Thermo Fisher Scientific for Cryo-EM

[ccp4bb] Cryo-EM acces for Covid-19 projects at CBI / IGBMC, Strasbourg

Dear all, this is to announce that we are opening our cryo-EM facilities for Covid-19 projects at the Centre for Integrative Biology, Illkirch/Strasbourg, France, which hosts the French and European Infrastructures for Integrated Structural Biology, FRISBI, Instruct-ERIC and iNext-Discovery.

[ccp4bb] Cryo-EM postdoc position in Shanghai

Shanghai Institute of Plant Physiology and Ecology (SIPPE) is a top plant research institute in China and internationally competitive. SIPPE focuses on plant genetics, development, physiology, and plant-environment interactions to answer important questions in both basic science and applied

[ccp4bb] Cryo-EM sample preparation workshop at the ESRF / 9th to 11th of June 2020

Dear All, We are pleased to announce our 2.5-day practical workshop on sample preparation for single particle cryo-EM from *June**9^th to 11^th2020*. This is the fourth of a series of practical hands-on workshops and is aimed at PhDs, PostDocs and scientists new to the field of single

[ccp4bb] Cryo-FIB/SEM & Cryo-ET postdoctoral positions in New York, $60 - 80K per year

The Fitzpatrick lab at Columbia's Zuckerman Institute has multiple openings for postdoctoral research associates to lead projects combining serial-section electron microscopy, correlative light/electron microscopy, advanced cryo-focused ion beam specimen preparations, cryo-electron tomography and

[ccp4bb] Cryo-Electron Microscopist opening at Novartis/GNF

The Genomics Institute of the Novartis Research Foundation (San Diego, CA) Structure Based Discovery group is seeking an Investigator level cryo-Electron Microscopist. Please see posting below: https://www.novartis.com/careers/career-search/job-details/288072BR Elena Menichelli, PhD

[ccp4bb] Cryo-EM specialist position at CBI / IGBMC, Strasbourg

Dear all, we are seeking a highly motivated cryo-EM specialist to join our cryo-EM facilities at the Centre for Integrative Biology, Strasbourg, France, which hosts the French and European Infrastructures for Integrated Structural Biology, FRISBI and Instruct-ERIC, and iNext-Discovery.

[ccp4bb] Cryo-EM staff scientist position available - SEMC, NYSBC, USA

Dear all, The New York Structural Biology Center (NYSBC) seeks an experienced microscopist to join the staff of the Simons Electron Microscopy Center (SEMC) (http://semc.nysbc.org). The facilities will soon include thirteen electron microscopes (including seven Titan Krios instruments),

[ccp4bb] cryo-EM facility manager position at UCSC

University of California, Santa Cruz is hiring a cryo-EM facility manager. See https://recruit.ucsc.edu/JPF00778 for details and to apply. Scientific responsibilities will include participating in structural biology research towards macromolecular structure

[ccp4bb] Cryo-EM Faculty Position

Dear CCP4 Community, Montana State University invites applications for a tenure-track faculty position in cryogenic electron microscopy. The position is formally a joint appointment between the Department of Chemistry and Biochemistry, and the Department of Microbiology and Immunology. The

[ccp4bb] Cryo-EM/X-ray/NMR Postdoctoral Position

*POSTDOCTORAL POSITION AVAILABLE * *STRUCTURAL BIOLOGY OF MEMBRANE REMODELING* A NIH-funded postdoctoral position is available immediately in the Ramachandran Lab at Case Western Reserve University (CWRU) to study the structural aspects of protein-mediated membrane remodeling during endocytic

[ccp4bb] Cryo-EM Junior Group Leader Position in Berlin

Dear PostDocs working in the field of cryo-EM, Please find below a job advertisement for a cryo-EM junior group leader position in Berlin at the MDC. Much looking forward to your applications ! All the best, Oliver The Max Delbrueck Center for Molecular Medicine (MDC) in Berlin, a

[ccp4bb] Cryo-EM beamline scientist position at CBI / IGBMC, Strasbourg

Dear all, here is an announcement for a cryo-EM beamline scientist position at the Centre for Integrative Biology, Strasbourg, France. With best regards, Bruno Klaholz Cryo-EM beamline scientist position: The IGBMC/CBI is seeking a experienced scientist to participate in running the

[ccp4bb] Cryo-EM Faculty Position

Dear CCP4 Community, Montana State University invites applications for a tenure-track faculty position in cryogenic electron microscopy (cryo-EM; single particle and/or tomography). The position is formally a joint appointment between the Department of Chemistry and Biochemistry, and the

[ccp4bb] Cryo-EM sample preparation workshop at the ESRF / 5th to 7th of November 2019 (last week before closure of registration)

Dear All, We are pleased to announce a 2.5 day practical winter workshop on sample preparation and negative staining for cryo-EM single particle data collection jointly organised by the ESRF, IBS, ILL and the EMBL Grenoble. This is the third of a series of practical hands-on workshops

[ccp4bb] Cryo-EM sample preparation workshop at the ESRF / 5th to 7th of November 2019

Dear All, We are pleased to announce a 2.5 day practical winter workshop on sample preparation and negative staining for cryo-EM single particle data collection jointly organised by the ESRF, IBS, ILL and the EMBL Grenoble. This is the third of a series of practical hands-on workshops

[ccp4bb] Cryo-FIB/SEM & Cryo-ET Staff Scientist position in New York

The Fitzpatrick lab, a member of the Zuckerman Institute at Columbia University, seeks to hire an electron microscopist (Staff Scientist) to join a CLEM / cryo-ET / SEM research program dedicated to imaging neurons, glia, and human brain tissue. The successful candidate will play a lead role in

[ccp4bb] Cryo-EM director position at the Hauptman-Woodward Medical Research Institute in Buffalo, NY.

Dear CCP4 readers, The Hauptman-Woodward Medical Research Institute has two current openings, one as a synchrotron macromolecular crystallographer at the IMCA beamline in Chicago, and announced today, for a cryo-EM center director based in Buffalo NY. Both will have the opportunity to interact

[ccp4bb] Cryo-EM Postdoctoral Position at Yale University

Dear all, There is an opening position in my lab. Please read the advertisement below: *Postdoctoral Research Position at Yale University School of Medicine* A postdoctoral research position is available in the laboratory of Dr. Wei Mi at Yale University School of Medicine. The research

[ccp4bb] Cryo-EM postdoc position, NNF-CPR, University of Copenhagen (Taylor group)

Dear all, We have a new and exciting postdoctoral position in my research group at NNF-CPR, University of Copenhagen, to study the structure and function of contractile injection systems targeting eukaryotic cells using integrative approaches (including cryo-EM). You will interact with a

[ccp4bb] Cryo-FIB/SEM & Cryo-ET Post-Doctoral Opportunities in New York

The Fitzpatrick lab, a member of the Zuckerman Mind Brain Behavior Institute at Columbia University, has multiple openings for postdoctoral research associates to lead projects combining serial-section electron microscopy, correlative light/electron microscopy, advanced cryo-focused ion beam

[ccp4bb] Cryo-EM Facility Manager Position available at CUHK(SZ)

The Chinese University of Hong Kong, Shenzhen CUHK(SZ) seeks for a full-time Cryo-EM Facility Manager. Dear all, The Cryo-EM platform at The Chinese University of Hong Kong, Shenzhen and the Kobilka Institute of Innovative Drug Discovery (KIIDD) seeks a Ph.D.-level scientist to serve as

Re: [ccp4bb] Cryo-EM Full Professor position at Strasbourg University

Dear all, kind reminder for those interested to apply for the cryo-EM full professor position in Strasbourg, the deadline for the registration via the Galaxie Web site is approaching (March 26th 2019). Please note that there is either a) a qualification step to have done already, or b) no need

[ccp4bb] Cryo-EM Data Processing Workshop, May 6-9, 2019 - Structural Biology Symposium, May 4, 2019

Cryo-EM Data Processing Workshop This 4-day satellite Cryo-EM Data Processing Workshop (May 6-9, 2019) will focus on EMAN2, SPHIRE, CHIMERA, COOT and PHENIX. This marks the rebirth of a long series of EMAN2 workshops previously hosted by the NCMI in Houston over the last 20

[ccp4bb] Cryo-EM Specialist position at the Harvard Cryo-EM Center for Structural Biology

Dear colleagues, A cryo-EM specialist position at the Harvard Cryo-EM Center for Structural Biology is available now. See below for detailed job description and how to apply. Please share this message with those who are interested. Your help is greatly appreciated. Cryo-EM Specialist position

[ccp4bb] Cryo EM director position at NYU

Hi All: NYU, located in New York City, is recruiting a cryo EM director (research track faculty position), and we would love to hear from you. Details below. Please don’t hesitate to email me directly with any questions, and please apply if interested! Best, Gira

[ccp4bb] Cryo EM at ETH

https://www.ethz.ch/content/dam/ethz/special-interest/dual/scopem/PDFs/Senior%20scientist%20-%20expert%20in%20Cryo%20Electron%20Microscopy%20(002).pdf To unsubscribe from the CCP4BB list, click the following link:

[ccp4bb] cryo-EM training videos

Dear colleagues: For those of you interested in cryo-EM, Matthijn Vos and I would like to announce that we have just made a large set of new training videos public covering the operation of Arctica and Titan Krios microscopes. The videos are part of an ongoing expansion of the original

Re: [ccp4bb] [EXTERNAL] [ccp4bb] cryo suggestions for lithium sulphate xt condition containing 1.8 - 2 M Li2SO4

ab (LBSF): https://tinyurl.com/ouhsclbsf > -- > *From:* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Almudena > Ponce Salvatierra [maps.fa...@gmail.com] > *Sent:* Tuesday, October 30, 2018 6:44 AM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [EXTE

Re: [ccp4bb] [EXTERNAL] [ccp4bb] cryo suggestions for lithium sulphate xt condition containing 1.8 - 2 M Li2SO4

Salvatierra [maps.fa...@gmail.com] Sent: Tuesday, October 30, 2018 6:44 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [EXTERNAL] [ccp4bb] cryo suggestions for lithium sulphate xt condition containing 1.8 - 2 M Li2SO4 Dear all, I have looked into the archive and I've tried to collect suggestions for cryo

[ccp4bb] cryo suggestions for lithium sulphate xt condition containing 1.8 - 2 M Li2SO4

Dear all, I have looked into the archive and I've tried to collect suggestions for cryo protecting crystals that grew in 1.8 M Lithium sulphate containing mother liquor or 2M. What would make more sense? Transferring it into a cryo solution that consists of mother liquor and a higher

[ccp4bb] cryo-EM position at ILL/PSB, Grenoble, France - application deadline on 4 November

Dear all, a Tenure-track Research Lectureship in Cryo-Electron Microscopy (Fixed term for 5 years) is currently available at the Institut Laue Langevin, Grenoble, France. Deadline for application is 4 NOVEMBER.

[ccp4bb] Cryo-EM Post-Doctoral Opportunities in New York

The Fitzpatrick lab, a member of the Zuckerman Mind Brain Behavior Institute at Columbia University, has 5 openings for postdoctoral research associates funded by a recent NIH U01 award to study the structural biology of tau and TDP-43 in neurodegenerative diseases. Situated in the heart of

[ccp4bb] Cryo-EM virology faculty position at UW-Madison

Dear Colleagues: The University of Wisconsin-Madison Institute for Molecular Virology (IMV), in collaboration with the Department of Biochemistry or the Department of Plant Pathology, seek to hire a tenure-track (junior) Assistant Professor to develop a collaborative cutting-edge research

Re: [ccp4bb] Cryo-EM Full Professor position at Strasbourg University

Dear all, kind reminder for those interested to apply for the cryo-EM full professor position in Strasbourg, the deadline for the pre-registration to the qualification step is approaching (14.10.2018). If you are interested or have questions about the procedure please write to the contact

Re: [ccp4bb] cryo-EM

Hi Xavier, > is there some kind of general trend if not consensus as how to refine > cryo-EM structures? > Not really consensus, but my clearly biased contribution to the topic: I'd say basic common sense for refinement applies: - optimally sharpen the map (

[ccp4bb] cryo-EM

Dear Xavier, in addition to Rob and Israel's excellent suggestions you may also want to have a look at Garib's account in Chapter 11 here: https://www.sciencedirect.com/bookseries/methods-in-enzymology/vol/579 Best wishes, Arjen On Friday, 31 August 2018, F.Xavier Gomis-Rüth wrote: > Dear

Re: [ccp4bb] cryo-EM

did for one of our structures: https://www.nature.com/protocolexchange/protocols/6365 HTH, Bruno From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Israel Sanchez Sent: Friday, August 31, 2018 2:32 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] cryo-EM Dear Xavier

Re: [ccp4bb] cryo-EM

-- From: "F.Xavier Gomis-Rüth" To: CCP4BB@JISCMAIL.AC.UK Sent: 31/08/2018 12:52:48 Subject: [ccp4bb] cryo-EM Dear all, is there some kind of general trend if not consensus as how to refine cryo-EM structures? What do people do for maps in the 3-4Å range, pure RSR against the unt

[ccp4bb] cryo-EM

Dear all, is there some kind of general trend if not consensus as how to refine cryo-EM structures? What do people do for maps in the 3-4Å range, pure RSR against the untouched map and that's it? Reciprocal space refinement against the backtransformed structure factors from the map but

[ccp4bb] Cryo-EM Full Professor position at Strasbourg University

INSTITUTE OF GENETICS AND MOLECULAR AND CELLULAR BIOLOGY CALL FOR APPLICATIONS FOR A PROFESSOR AND GROUP LEADER POSITION IN MOLECULAR AND CELLULAR CRYO ELECTRON MICROSCOPY A full professor position is open at the Strasbourg University to establish a research team in molecular or cellular cryo

[ccp4bb] Cryo-EM beamline scientist position at CBI / IGBMC, Strasbourg

Dear all, here is an announcement for a cryo-EM beamline scientist position at the Centre for Integrative Biology, Strasbourg, France. With best regards, Bruno Klaholz Cryo-EM beamline scientist position: The IGBMC/CBI is seeking a experienced scientist to participate in running the

[ccp4bb] Cryo-EM structures sought for CASP13

Critical Assessment of Techniques for Protein Structure Prediction (CASP) community experiments aim to advance the state of the art in protein structure modeling. Every other year since 1994, CASP collects information on soon-to-be released experimental structures, passes on sequence data to

Re: [ccp4bb] cryo-em data conversion

l.com> Sent: 08 February 2018 20:46:54 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] cryo-em data conversion Dear Members, Sorry for if the question is off topic. Can someone suggest the easiest way to convert .tif file (raw data) into .mrcs format and also need to create a .star file to use Relion

[ccp4bb] cryo-em data conversion

Dear Members, Sorry for if the question is off topic. Can someone suggest the easiest way to convert .tif file (raw data) into .mrcs format and also need to create a .star file to use Relion for the following steps? Thanks for your help. Best regards, Hena

[ccp4bb] Cryo-electron Tomography Specialist / Assistant Professor (0.8-1.0 FTE)

Sent on behalf of Friedrich Foerster: The Department of Chemistry at Utrecht University is seeking a highly-qualified colleague to strengthen the research activities of the cryo-electron microscopy (cryo-EM) group in the field of in situ structural biology. The successful applicant has a

[ccp4bb] Cryo-EM Professor position at Strasbourg University

Dear all, the University of Strasbourg, France, is opening a call for a Professor and group leader position in cryo-EM. Interested applicants please see below for further informations. Best regards, Bruno Klaholz INSTITUTE OF GENETICS AND MOLECULAR AND CELLULAR BIOLOGY CALL FOR

[ccp4bb] Cryo-EM postdoctoral postion in Sydney Australia

Dear all, I’m currently looking for a postdoc to join my group at the Victor Chang Cardiac Research Institute in Sydney, Australia. The successful candidate will join a small growing group, and investigate the structure and function of large protein complexes using cryo-EM. Experience in

[ccp4bb] Cryo-EM Research Scientist position at Imperial College London & MRC LMS

The MRC LMS & Imperial College is looking for a Cryo-EM research Scientist. Your main role will be to manage the EM requirements of the institute. Electron microscopy has been identified as a key technology at the ICL/LMS to support investigation into epigenetic mechanisms, large molecular

[ccp4bb] Cryo-EM symposium, 4-5 September 2017, Delft University of Technology

Dear colleagues, We are pleased to announce the International Cryo-EM Symposium that will take place from 4-5 September 2017 in Delft, the Netherlands. With the aim to share the latest developments in 3D cryo-electron microscopy (EM) with scientists and students at the Delft University of

[ccp4bb] Cryo-EM School2017 in Pavia: Deadline Extended, but few places remaining

Dear all, Few places are still available for the AIC School "Bridging the gap between Cryo-EM and Crystallography". http://www.cristallografia.org/aicschool2017 We have decided to extend the bursary registration deadline until May 30th, 2017. We wish to remind applicants that have received

Re: [ccp4bb] Cryo-em model building with Balbes

/training/icknield_2017/icknield_2017.php HTH m -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of cheng tat cheung Sent: 25 April 2017 16:18 To: ccp4bb Subject: Re: [ccp4bb] Cryo-em model building with Balbes phenix.map_to_structure_factors can do

Re: [ccp4bb] Cryo-em model building with Balbes

phenix.map_to_structure_factors can do the conversion, and then all the crystallographic tools for model building should be ready to use. Tat Sent from my iPhone > On 25 Apr 2017, at 10:36 AM, KL Ho > <05c3a58110fa-dmarc-requ...@jiscmail.ac.uk> wrote: > > Dear All, > > We are interested

[ccp4bb] Cryo-EM positions at EMBL

Dear all, We invite applications for postdoc and support scientist positions in the Sachse laboratory at EMBL: Postdoctoral Fellow – Structural membrane biology cryo-EM http://s.embl.org/HD01058 Research Technician – Support scientist structural biology

[ccp4bb] Cryo-EM Symposium, 6-7 July 2017, European Photon & Neutron Campus, Grenoble, France

Dear all, We are pleased to announce the Cryo-Electron Microscopy Symposium that will take place from 6-7th July, 2017 in Grenoble, France. The aim of this symposium is to promote the exciting opportunities in structural biology opened by the advances in cryo-electron microscopy and by the

Re: [ccp4bb] Cryo-EM beamline scientist position at CBI, Strasbourg

Dear all, kind reminder for the cryo-EM beamline position at the Centre for Integrative Biology. Application deadline is next week 15.1. Best regards, Bruno Klaholz De : Bruno KLAHOLZ Envoyé : vendredi 9 décembre 2016 17:51 À :

[ccp4bb] Cryo-EM in Australia: group-leader and staff-scientist positions

Dear cryo-EM community, Two continuing, full time positions are available at the University of Wollongong in Australia: Staff Scientist (Cryo-Electron Microscopy): http://uow.employment.com.au/jobs/Staff-Scientist--Cryo-Electron-Microscopy-/2487 Senior Lecturer / Associate Professor

Re: [ccp4bb] cryo for crystals with CTAB as precipitant

of Alejandro Madrigal Carrillo [amadri...@email.ifc.unam.mx] Sent: Monday, February 22, 2016 11:00 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] cryo for crystals with CTAB as precipitant Dear All, I got crystals using CTAB 10mM as precipitant, but I have had problems with the cryoprotectant solution

Re: [ccp4bb] Cryo-EM

Hi everyone Thanx a lot..Your suggestions are really informative and a valuable source of knowledge.. regards Faisal On 5/20/15, Takanori Nakane takanori.nak...@bs.s.u-tokyo.ac.jp wrote: Hi Faisal, Recordings of MRC-LMB EM-course last year are available at

[ccp4bb] Cryo-EM

Hi everyone A bit off topic..but..I request you to please suggest me some good readings related to Cryo-EM.. Thanx in advance -- Regards Faisal School of Life Sciences JNU

Re: [ccp4bb] Cryo-EM

Hi Faisal, I guess a start for you would be to go here: http://www.ccpem.ac.uk/ and subscribe to that mailing list :-) Best regards, Folmer 2015-05-19 9:01 GMT+02:00 Faisal Tarique faisaltari...@gmail.com: Hi everyone A bit off topic..but..I request you to please suggest me some good

Re: [ccp4bb] Cryo-EM

Hi Faisal, * This is a good introduction for a non-microscopist: Cryo-electron microscopy--a primer for the non-microscopist. Milne JL1, Borgnia MJ, Bartesaghi A, Tran EE, Earl LA, Schauder DM, Lengyel J, Pierson J, Patwardhan A, Subramaniam S. http://www.ncbi.nlm.nih.gov/pubmed/23181775 * A

Re: [ccp4bb] Cryo-EM

Hi Faisal, There are some good video introduction available too: https://www.youtube.com/watch?v=nkGRhYv01ag HTH, Partha On Tue, May 19, 2015 at 3:01 AM, Faisal Tarique faisaltari...@gmail.com wrote: Hi everyone A bit off topic..but..I request you to please suggest me some good readings

Re: [ccp4bb] Cryo-EM

Hi Faisal, Two recent reviews well worth reading are below: A Primer to Single-Particle Cryo-Electron Microscopy. Cheng Y, Grigorieff N, Penczek PA, Walz T http://www.ncbi.nlm.nih.gov/pubmed/25910204 Single-Particle Cryo-EM at Crystallographic Resolution. Cheng Y.

Re: [ccp4bb] Cryo-EM

Hi Faisal, if you are particularly interested in refinement of atomic models into cryo-EM maps then you may check this too: http://phenix-online.org/presentations/real_space_refine.pdf (Of course by no means I claim this being a good reading!) Good luck, Pavel On Tue, May 19, 2015 at 12:01

Re: [ccp4bb] Cryo-EM

Hi Faisal, Recordings of MRC-LMB EM-course last year are available at http://www2.mrc-lmb.cam.ac.uk/groups/scheres/impact.html Best regards, Takanori Nakane On 2015/05/20 2:36, Yi-Wei Chang wrote: Hi Faisal, Here is a new series of lecture video called Getting Started in Cryo-EM made by

Re: [ccp4bb] cryo condition

Along with all the excellent suggestions so far you can also try no cryoprotectant at all: If you harvest your crystal on a mesh loop and remove all the mother liquor the crystal lattice itself will act as a cryoprotectant as long as the solvent channels are smaller than 40A in diameter (they

Re: [ccp4bb] Cryo protection

Cryo On 5 May 2015 13:51, faisaltari...@gmail.com wrote: Thank you everyone..I have got some hints from this discussion and will definitely try some of them to check its efficacy for my case...Thanx again for your valuable suggestions.. On 5 May 2015 13:48, Anthony Savill

Re: [ccp4bb] cryo condition

Hi Faisal, Did you try to simply raise the Sokalan CP7 percentage? Additives like Glycerol can increase the solubility of proteins, in that case you have to counteract by increasing also the precipitant concentration. If your crystals crack, a likely reason is osmotic shock. Particularly

[ccp4bb] cryo condition

Hello everyone Can anybody suggest me a cryo condition for a crystal obtained in MIDAS screen of Molecular Dimension: G1 0.1MTris8.0G10.1Mpotassium chloride25% v/vSOKALAN®CP7 0.1MHEPES7.0 G20.3Mammonium formate20% v/vSOKALAN® CP 5 0.1MHEPES7.0 Crystals are in beautiful cuboid shaped but all

Re: [ccp4bb] cryo condition

like you describe. GL Bert From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Faisal Tarique [faisaltari...@gmail.com] Sent: Monday, May 04, 2015 7:43 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] cryo condition Hello everyone Can anybody

Re: [ccp4bb] cryo condition

of Roger Rowlett rrowl...@colgate.edu Sent: Tuesday, 5 May 2015 7:22 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] cryo condition We rarely use glycerol anymore, because it seems to fail so often for many of our current proteins. Try glucose, 25-30%. This is most conveniently done

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