[ccp4bb] Generating rotation/translation matrices for biological assemblies

2019-02-02 Thread Klontz, Erik
Hi all, I'm working on a protein that I believe is tetrameric based on AUC, gel filtration, and crystallography. However, although my asymmetric unit has 4 chains, I cannot form the tetramer within the asymmetric unit. Instead, the asymmetric unit has two half-tetramers ('dimers'), and each fu

Re: [ccp4bb] Generating rotation/translation matrices for biological assemblies

2019-02-02 Thread Mooers, Blaine H.M. (HSC)
Generating rotation/translation matrices for biological assemblies Hi all, I'm working on a protein that I believe is tetrameric based on AUC, gel filtration, and crystallography. However, although my asymmetric unit has 4 chains, I cannot form the tetramer within the asymmetric uni

Re: [ccp4bb] Generating rotation/translation matrices for biological assemblies

2019-02-02 Thread Ricardo Padua
Hi Erik, Have you tried Achesym? http://achesym.ibch.poznan.pl Kowiel, M., Jaskolski, M. & Dauter, Z. (2014). ACHESYM: an algorithm and server for standardized placement of macromolecular models in the unit cell. Acta Cryst. D70, doi:10.1107/S1399004714024572. Best Ricardo Padua On Sat, Feb 2

Re: [ccp4bb] Generating rotation/translation matrices for biological assemblies

2019-02-02 Thread Jose Duarte
Try EPPIC (http://eppic-web.org), it will give you all possible symmetric assemblies in your crystal and their constituent interfaces, listing the symmetry operators too. See some help here http://eppic-web.org/ewui/#help Jose On Sat, 2 Feb 2019 at 16:42, Ricardo Padua wrote: > Hi Erik, > > Hav

Re: [ccp4bb] Generating rotation/translation matrices for biological assemblies

2019-02-03 Thread Bernhard Rupp
://www.ruppweb.org/new_comp/spacegroup_decoder.htm Standard settings only. Best, BR From: CCP4 bulletin board On Behalf Of Klontz, Erik Sent: Saturday, February 2, 2019 22:05 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Generating rotation/translation matrices for biological assemblies Hi all

Re: [ccp4bb] Generating rotation/translation matrices for biological assemblies

2019-02-03 Thread Eleanor Dodson
comp/spacegroup_decoder.htm > > Standard settings only. > > > > Best, BR > > > > *From:* CCP4 bulletin board *On Behalf Of *Klontz, > Erik > *Sent:* Saturday, February 2, 2019 22:05 > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] Generating rotation/t

Re: [ccp4bb] Generating rotation/translation matrices for biological assemblies

2019-02-03 Thread Klontz, Erik
ecoder.htm Standard settings only. Best, BR From: CCP4 bulletin board mailto:CCP4BB@JISCMAIL.AC.UK>> On Behalf Of Klontz, Erik Sent: Saturday, February 2, 2019 22:05 To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Generating rotation/translation matrice

Re: [ccp4bb] Generating rotation/translation matrices for biological assemblies

2019-02-03 Thread Eleanor Dodson
contains a rotation matrix and a translation > vector that should correspond to the same solution. > > > Thanks everyone! > > Erik > > ------ > *From:* CCP4 bulletin board on behalf of Eleanor > Dodson <176a9d5ebad7-dmarc-requ...@jisc