Hello everyone, I need your suggestion for slowing down crystallization for my
proteinmy protein got hit in PEG/ION #5 ( 0.2 M MgCl2, 20% PEG 3350, pH 5.9),
but it crystallize too fast. In 1 hr I can see tons of tiny needles. Can anyone
give me some suggestion on how to slow down the process? I
On Mon, Feb 25, 2013 at 8:02 AM, lei feng spartanfeng...@hotmail.com wrote:
I need your suggestion for slowing down crystallization for my protein
my protein got hit in PEG/ION #5 ( 0.2 M MgCl2, 20% PEG 3350, pH 5.9), but
it crystallize too fast. In 1 hr I can see tons of tiny needles.
Can
Subject: [ccp4bb] How to slow down crystallization? Need hep!
To: CCP4BB@JISCMAIL.AC.UK
Hello everyone,
I need your suggestion for slowing down crystallization for my protein
my protein got hit in PEG/ION #5 ( 0.2 M MgCl2, 20% PEG 3350, pH 5.9), but it
crystallize too fast. In 1 hr I can see
Le 25/02/13 17:02, lei feng a écrit :
Hello everyone,
I need your suggestion for slowing down crystallization for my protein
my protein got hit in PEG/ION #5 ( 0.2 M MgCl2, 20% PEG 3350, pH 5.9),
but it crystallize too fast. In 1 hr I can see tons of tiny needles.
Can anyone give me some
a. Go with phase diagram gradually changing concentrations of protein,
precipitant, salt concentrations and pH. Find where you are going out of
crystallisation. Define region for streak seeding.
b. Screen for ratios of precipitant to protein from 1:9 to 9:1.
Dr Felix Frolow
Professor of
Hi Lei Fang,
I cannot tell what technique you are using for crystallization. Also, many
details like protein concentration, temperature of crystallization etc. are
missing in your email so it's hard to guess what's going on.
In any case, here are my thoughts. I used to get a shower of needles
Try using dioxane as an additive. I'd suggest adding 5- 15% dioxane into your
well solution. This should inhibit nucleation resulting in fewer, larger
crystals.
On 25/02/13, Felix Frolow mbfro...@post.tau.ac.il wrote:
a. Go with phase diagram gradually changing concentrations of
