Hi Jay,
Maybe try to use the the rhombohedral setting (space group R32:R, with
unit cell 229,229,229,81,81,81 ) rather than the hexagonal one used now.
Depending on the fft algorithm used to compute structure factors for
your partial model, this can save you a chuck off memory/time.
You can
The cell is quite big. 300 x 300 x 450 A, 90, 90, 120 (R32). I'm using the
entire resolution range at the moment, but I've just set up another job with
a reduced resolution range. So we'll see how this goes. I only get this
error message when I do a run with Phaser with this partial known solu
Jay Thompson wrote:
Hi,
I have a question with molecular replacement using Phaser. I'm trying
to solve a complex and I have a partial molecular replacement solution
solved using another program. This solution is correct and makes up
~50% of the entire complex. I wanted to fix this solution
Hi,
what resolution range do you use? You can try reducing it a little.
How big is your cell?
Tim
On Friday 18 May 2007 13:46, Jay Thompson wrote:
> Hi,
>
> Thanks for the suggestions and quick reply. Suggestions work great!
>
> But I have another problem and looking back at the ccp4bb, I see t
Hi,
Thanks for the suggestions and quick reply. Suggestions work great!
But I have another problem and looking back at the ccp4bb, I see that Elenor
had a similar problem late last year. The error message is as follows:
--
OUT OF MEMORY ERROR: St9bad_alloc
--
Hello,
you could try to let phaser search for the first fragment, too. That way it
should produce a file ending with '.sol' which you can pass to phaser for the
second search (bottom at the ccp4-gui, "Define search sets...").
Otherwise, if you do not want to move the first fragment, both the Eu
What about giving the correctly positioned partial solution,
and fixed angles/translation of 0,0,0,0,0,0 for that part?
Jay Thompson wrote:
Hi,
I have a question with molecular replacement using Phaser. I'm trying
to solve a complex and I have a partial molecular replacement solution
solved
Hi,
I have a question with molecular replacement using Phaser. I'm trying to
solve a complex and I have a partial molecular replacement solution solved
using another program. This solution is correct and makes up ~50% of the
entire complex. I wanted to fix this solution and search for another
