Acoot
You may be interested in some data-mining that we did a few years ago
http://www.douglas.co.uk/PDB_data.htm#Acid-base
We didn't see any obvious correlations
On 28 October 2012 03:00, Acoot Brett acootbr...@yahoo.com wrote:
Dear All,
A protein crystal can be got at pH 5 or 8, or a pH
An example for how crystallization at non-physiological pH (and
non-physiological concentrations and non-physiological environmental) may
influence behavior of the protein is the Botulinum A LC protease. Under normal
conditions (upon endocytosis of a few molecules at best into the nerve cell),
Hi Acoot,
Here are some examples you can look at:
PDB: 1X0I (crystal structure of bacteriorhodopsin (acid blue form) at pH 2)
PDB: 1X0K (crystal structure of bacteriorhodopsin at pH 10)
PDB: 2W2E (crystal structure of aquaporin at pH 3.5)
PDB: 1YMG (crystal structure of aquaporin at pH 10)
Dear All,
A protein crystal can be got at pH 5 or 8, or a pH with much extreme value.
What will be the relatively extreme pH value to get the crystal on the protein
structure solved based on the crystal got? I mean usually we regard the
physiological pH as 7. If a crystal was got at pH 5, the
