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De: CCP4 bulletin board em nome de DUMAS Philippe
(IGBMC)
Enviado: quinta-feira, 28 de março de 2019 12:48
Para: CCP4BB@JISCMAIL.AC.UK
Assunto: Re: [ccp4bb] Interesting pattern on a crystallization drop
Le Jeudi 28
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> From: CCP4 bulletin boar
arch 28, 2019 1:07
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop Neat!
Looks like multiple adjacent bubbles that were initially touching but
eventually shrunk down to the central cores - the connectors are protein
filaments (skin on the bubbles) left
/anafar>
From: CCP4 bulletin board on behalf of Artem Evdokimov
Sent: Thursday, March 28, 2019 1:07
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop
Neat!
Looks like multiple adjacent bubbles that were initiall
Hi Beatriz,
Very interesting indeed. Couldn't it be the ghost printing of some mechanical
part used to make the mould of the crystallization tray ?
All the best,
Philippe
Philippe BENAS, Ph.D.
ARN UPR 9002 CNRS
IBMC Strasbourg
15, rue René Descartes
F-67084 STRASBOURG cedex
+33.3.8841.7109
E-m
this email.*
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> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of
> *LEGRAND
> Pierre
> *Sent:* Thursday, 28 March 2019 9:13 AM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] Interesting pattern on a crystallization drop
>
>
C.UK] On Behalf Of LEGRAND
Pierre
Sent: Thursday, 28 March 2019 9:13 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop
Dear Beatriz,
Nice drops :-))
Could it be that there is a reaction going on in these drops ?
The conditions are quite "exotic
Dear Beatriz,
Nice drops :-))
Could it be that there is a reaction going on in these drops ?
The conditions are quite "exotic" with possibilities of coordination or
oxydoreduction (Co2+/Co3+) or polymerization...
Do you have reductants with the protein buffer ?
Is the protein an enzyme or a metal
)3316-3225/2104-3438
De: Ailong Ke
Enviado: quarta-feira, 27 de março de 2019 17:06:01
Para: Beatriz Gomes Guimaraes
Cc: CCP4BB@JISCMAIL.AC.UK
Assunto: Re: [ccp4bb] Interesting pattern on a crystallization drop
Hi Beatriz,
Were the drops set up by hand or by a
Hi Beatriz,
Were the drops set up by hand or by a robot? If the latter, what kind of robot?
are they hanging drops or sitting drops, and do the bubbles form on the glass
surface of at the bottom of your drop?
AK
On Mar 27, 2019, at 2:44 PM, Beatriz Gomes Guimaraes
mailto:beatriz.guimar...@fio
It's a crystal with a very large lattice.
JPK
+
Jacob Pearson Keller
Research Scientist / Looger Lab
HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
Desk: (571)209-4000 x3159
Cell: (301)592-7004
+++
I'm guessing quite big drops on a somewhat hydrophobic surface? Also guessing
that the protein foams quite a bit, and that there were 6 (or four) more or
less equal size bubbles that took up almost the whole drop when it was set up.
Bubbles tend to pack quite efficiently
(https://physicsworld.c
: DUMAS Philippe (IGBMC)
Enviado: quarta-feira, 27 de março de 2019 16:34
Para: Beatriz Gomes Guimaraes
Cc: CCP4BB@JISCMAIL.AC.UK
Assunto: Re: [ccp4bb] Interesting pattern on a crystallization drop
Le Mercredi 27 Mars 2019 19:44 CET, Beatriz Gomes Guimaraes
a écrit:
In four days from now, I
Le Mercredi 27 Mars 2019 19:44 CET, Beatriz Gomes Guimaraes
a écrit:
In four days from now, I (everyone) would have taken this as a Photoshop joke...
Quite amazing! Is it reproducible?
What is the protein? Tubulin ? ::))
Philippe Dumas
> Dear all,
>
>
> I would like to share with you a surpri
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