..and we here in NZ have our own government elections this weekendfun
times all over the world :-)
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Tony Henwood
Sent: Tuesday, 4 November 2008 11:06 a.m.
To: Jerry Helisek; histonet@lists.utsouthwestern.e
MDS Analytical Technologies will be hosting the following seminar at the
Society for Neuroscience Meeting on Monday, November 12, 2008
Advances in LCM and Microgenomics Research
Speakers include:
*Charmaine Pietersen, Ph.D., McLean Hospital
"Gene expression analysis in homogenous single c
Thanks for the many replies however my objective hasn't been addressed.
For the supervisors; My point is not to mislead and claim ASCP certification
without having it. My question is to assist in rationalizing cost.
Disregarding a credible applicant who doesn't see the financial investment
toward
Rabbit anti-CD20, B cell (Lab Vision/NeoMarkers; cat. #: RB-9013-P;
Immunogen: synthetic peptide derived from C-terminus of human CD20 protein)
This antibody has worked on every mammalian species on which I've tried it.
Does not need ANY tissue pretreatment! Stains brilliantly.
Jan Shive
Kim,
That is my understanding as well.
Mary Abosso
From: [EMAIL PROTECTED] on behalf of Kimberly Tuttle
Sent: Mon 11/3/2008 12:08 PM
To: R C; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] ASCP HT maintenance fees
Really? I never pay to maintain
Thanks for the 'vote' of confidence. :)
Claire
>From down here in Australia, we hope it goes well.
We are thinking of you.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at we
>From down here in Australia, we hope it goes well.
We are thinking of you.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street,
I want to make you aware that JCAHO requires accredited facilities to undertake
"primary source verification" for anyone claiming to have professional
credentials. If I wanted to hire an applicant claiming to have histology
certification it is my responsibility as manager to verify with ASCP tha
Justin,
You have got to be kidding me...not a website to be pushing your political
rhetoric. I got enough political paper to recycle in my mail at home, now I
have to push the delete button.
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Justin Thomas
I believe that when we've had to do this in the past, we've done EIER
followed by HIER because that's the order it occurs on Ventana
instruments.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Origin
Hi folks,
I have a new antibody that seems to need both Protease digestion and
Citrate buffer antigen retrieval. Does anyone have any experience with
this double whammy approach and know if it works best to do EIER before
HIER or HIER before EIER. Perhaps it doesn't matter which is first?
Any
DO NOT POST POLITICAL OPINIONS ON HISTONET- THAT IS NOT WHAT IT IS FOR
On Mon, 3 Nov 2008, Yaskovich, Ruth A (NIH/NIDCR) [E] wrote:
I don't think the Histonet is appropriate for this type of Posting!
Ruth Yaskovich
N.I.H.
-Original Message-
From: Justin Thomas [mailto:[EMAIL PROTEC
I have been evaluating the Leica Bond IHC stainer and their tech support
Pauline Wong is absolutely wonderful. Will also be evaluating the Intellipath
by Biocare in December. Any comments greatly appreciated!
Thank you,
Angela
___
Histonet mailing
If you were certified in 2004 or after you need to turn in 36 credit hours of
continuing education in order to maintain your certification. You do not need
to be a member of ASCP to be certified, however you do get some free CE hours
with your membership. My certification was in 85, so yes, I am
Hopefully only until tomorrow. Perfect timing to bring it up is you ask
me. Not much time left to debate it on the histonet.
On 11/3/08, Carol Fields <[EMAIL PROTECTED]> wrote:
>
> This is totally not appropriate for the HistoNet. This fight would go
> on forever...
>
> Carole Fields, HT (
We were asked to do immunostains on slides where a particular cell line was
growing. Can anyone tell me the best way to fix these slides before
performing immunostains? We used to use acetone on cytospins but can't recall
if there is a better way.
thanks
Jim Vickroy BS, HT(ASCP)
Techni
Hi Richard,As Gundrun lined out, a sequential method with a heating step in
between to remove antibodies from the first staining sequence without affecting
the DAB precipitate, is a very safe way to combine two antibodies from the same
species. However, with this brown-red color combination one
Hi Histonetters!
I am looking for a good quality histopathological lab for animal tissues
in New Dehli, India. Please contact me if any one knows of a reputable,
high quality lab.
MaryAnn Dixon BS
Biological Scientist
Anatomic Pathology
UF Veterinary Medical Center
(352) 392-2235 Ext.
Really? I never pay to maintain HT certification. As far as I know theres a
ASCP membership fee, but you dont have to be a member to be certified. Am I
wrong here?
Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
Bal
curiously, the only other post on this list from this person was spam,
hahahahaha!
http://www.histosearch.com/histonet/Jun08A/RE.HistonetSUPPLYANDCHEMIA.html
0 for 2... nice!
Justin Thomas wrote:
Barack Obama will raise taxes on hardworking Americans to give a government
handout to the
This is just spam... Please delete this person.
Thanks
Raymond A. Lamb, Inc
Jerry Helisek
VP North America
[EMAIL PROTECTED]
5409 Lumley Road, Unit 102
Durham, North Carolina 27703
tel: 919.957.1964
fax: 919.957.1972
mobile: 919.264.7964
Skype ID:j
Hi Netters,
I'm stumped with a project and could really use your help. I have 3mm
punches of skin that I need to stain with PGP 9.5 but I am specifically
looking for the intraepidermal nerve fibers. The sections were given to
me fixed in formalin and then I made cryo buttons out of them using
Can someone assist me in rationalize the annual cost of maintining HT
certification (roughly $100 annually) and its benefit? Point accumulation is
generally low for classes you must pay for, and those who obtained
certification prior to 2004 are exempt. Should one not pay the annual fee,
certificat
Emily, I love your response. There is no way this Justin person is a histotech
because everyone knows how intelligent we are and we've all done our research
and made the decision that is best for us.
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Emily
Wow, where to start... lets see:
1) off-topic
3) factually incorrect
4) subjective and personally biased
5) abuse of ALL-CAPS
6) infuriatingly pandering
This inappropriate to send out to such a list, John. Shame on you, you
should know better.
Justin Thomas wrote:
Barack Obama will raise ta
Can we cut the messages on how this message wasn't appropriate???
Or, at least send them to the original person? I have what a dozen messages
exclaiming how inappropriate this message was!!! Frankly, the responses are
more inconvenient than the original message!
Malcolm L. McCallum
Associate
Honestly who cares. Let's just report to Listserv manager and move on.
--
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
I absolutely agree ... THIS IS NO PLACE TO POST YOUR OPINON concerning politics
!
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Yaskovich, Ruth
A (NIH/NIDCR) [E]
Sent: Monday, November 03, 2008 8:19 AM
To: [EMAIL PROTECTED]; his
I guess free speech is already dead. Go ask Joe the Plumber about his..
Justin, I suggest having chocolate chip cookies ready for when the KG.., oops,
the FBI knocks on your door.
- Original Message
From: Greg Dobbin <[EMAIL PROTECTED]>
To: histonet@lists.utsouthwestern.edu; [EMAIL
I received something similar but it was pro-Obama/anti-McCain on another
listserv. I guess both parties are guilty.
From: [EMAIL PROTECTED] on behalf of Greg Dobbin
Sent: Mon 11/3/2008 11:40 AM
To: histonet@lists.utsouthwestern.edu; [EMAIL PROTECTED]; [EMAIL PRO
I don't remember seeing this persons name on the Histonet before. Could
he be a Republican Party volunteer who joins various listservs for the
sole purpose of garnering support for Mr. McCain??
It certainly would not surprise me. The fact that so many of us have
read it already tells me he's accomp
Gee, I really didn't know who I was going to vote for, being completely
unaware of both candidates' platforms the day before I vote, but this random
email to histonet totally swayed my decision!
What a relief to know people like Justin care enough to tell me how to vote!
Emily
--
"You would know
Now, I think I've seen everything!! I can't believe you would use this venue
for this trash.
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Justin Thomas
Sent: Monday, November 03, 2008 10:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet]
And this deals with the sharing of Histology ideas How?
Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220
From: [EMAIL PROTECTED] [EMAIL PROTECTED] On Behalf Of Edwards, R.E. [EMAIL
PROTECTED]
Se
This is totally not appropriate for the HistoNet. This fight would go
on forever...
Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
[EMAIL PROTECTED]
CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by
Northside Hospital. It ma
This is very much uncalled for. This is not the place for political views. Keep
them to yourself.
Beatrice DeBrosse-Serra
Pathology Scientist
Pfizer Global Research & Development
CB4, 2150
10646 Science Center Drive
San Diego, CA 92121
Phone# 858-622-5986
Fax# 858-678-8290
-Original Mess
I don't think the Histonet is appropriate for this type of Posting!
Ruth Yaskovich
N.I.H.
-Original Message-
From: Justin Thomas [mailto:[EMAIL PROTECTED]
Sent: Monday, November 03, 2008 11:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Presidential Voting Infomation
Bar
This totally inappropriate for HistoNet or any professional list server
Pamela A Marcum
University of Pennsylvania
School of Veterinary Medicine
Comparative Orthopedic Laboratory (CORL)
382 W Street Rd
Kennett Square PA 19438
610-925-6278
-Original Message-
From: [EMAIL PROTECTED]
[mailt
You cannot do what Paula recommends below without extra blocking
steps, if the primaries are raised in the same species as the second
secondary is raised. This is a rough outline of what I recommend:
1st primary antibody (X-anti-Y)
1st biotinylated secondary antibody (Fab anti-X)
Streptavidin-H
Thanks but no thanks..
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Justin Thomas
Sent: 03 November 2008 16:11
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Presidential Voting Infomation
Barack Obama will raise taxes on hardworking America
Barack Obama will raise taxes on hardworking Americans to give a government
handout to the 40% of Americans who pay no income taxes.
John McCain and Sarah Palin have an economic plan that celebrates the American
dream of opportunity, not government giveaways. In this country, we believe in
sprea
Hello,
I am looking for assays (either chromogenic or fluorescence based) for both
complement activation and thrombin generation for a proposal. The ones
suggested by the sponsor are not available in this country. Any
suggestions/sources?
Thanks,
Albert
**Plan your next getaway
We have had much success with the Biocare line of double staining cocktails and
ancillaries. Very easy to do
Loralee McMahon, HTL (ASCP)
ICC Supervisor
University of Rochester
Department of Pathology
(585) 275-7210
From: [EMAIL PROTECTED] on behalf of Edw
Hopefully Chris van der Loos is looking in on this and will send the person
who needs to do double IHC. He just published a very useful method in
Journal of Histotechnology on this subject where the first antibody complex
is removed, leaving behind chromogen after a retrieval method followed by
We do an automated doublestain, that works with Peroxidase-DAB and Alk.
Phosphatase-Red. The critical point is, to perform a second HIER-step
between the two detection-sequences.
The fixed specimen don't suffer from the second heating, but the native
antibodies are destroyed and are not detectable
Incubate with one antibody and use DAB as the chromagen, then go back and
incubate with the second antibody and use a colored chromagen such as AEC.
Paula
- Original Message
From: "Edwards, R.E.." <[EMAIL PROTECTED]>
To: histonet
Sent: Monday, November 3, 2008 8:08:46 AM
Subject: [
OK chaps, which in your opinion is the best double labelling method when one
is using primary antibodies raised in the same species, I am aware that
Vector labs supply such a kit; many thanks.
Cheers
Richar
Dear Histonetters,
I'm looking for (peptide) antibodies against known cytoplasmic sequences of
CD19 and/or CD20 (preferably murine or human antigen, but basically any
mammalian species would do). In other words, I need to find
mammal-crossreactive reagents for these targets. Any tips are greatly
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