Good morning,
I was wondering if someone uses tap water to blue their slides after
Hematoxyline. If yes, do you use warm or cold water and for how long?
Thanks,
Eva
Georgetown University
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You could also try to add some ammonium hydroxide in tap water after the Mayers
and before the tap water rinse. This helps us in the bluing on our mayers HE
frozen tissue stain line. Add approx 5ml to 150ml of water from 30 sec to 1 min
you will have to play with it a little . This blues our
Hello,
I rinse in tap water, but blue in 1-2% ammonium hydroxide for ~ minute, then
rinse again in tap water.
Paula
From: Eva Permaul e...@georgetown.edu
To: histonet@lists.utsouthwestern.edu
Sent: Monday, January 12, 2009 8:35:15 AM
Subject: [Histonet]
Hi TF:
Your problem should not be happening.
Sounds like poor perfusion followed by immersing the whole brain (with
the wound deep in the interior) in a small volume of fix at 4 degrees C.
What fixative are you using?
Geoff
TF wrote:
Hi, I just want to fix the damaged brain tissue (2-48
Bluing in tap water is quite often done but has the disadvantage that your
protocol cannot be repeated in other Lab because not all tap waters have the
same composition of bluing
quality.
As any other chemical reaction it is better to blue in warm tap water (it will
take less time) but you can
I blue in gently running tap water for the same time as I stained
with the haematoxylin. Glasgow water is pure, gentle, and soft and comes
from Loch Katrine.
Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
Glasgow,
G12 8QQ.
4% PFA
so u mean I should expose more of the damaged site (it is at the bottom of the
brain, or olfactory bulb)
and immerse the whole brain in a larger volume of fixatives?
I think PFA penetrate quite fast, and I took all the skins muscles off the
skull before put the who brain with skull into
What's your New Year's Resolution?
Of course I resolve that I will try to eat right and exercise. I also
intend to learn to play piano.
But. my most important resolution is to help even more histo techs find the
right opportunity in the right place at the right time.
In 2008 I helped people
Thanks, Dont you think high pressure during perfusion leads to tissue swelling
and disrupted morphology?
2009-01-13
TF
发件人： Charles Scouten
发送时间： 2009-01-13 00:02:40
收件人： ti...@foxmail.com
抄送： histonet
主题： RE: Re: [Histonet] Fixation of the damaged brain tissue
Formaldehyde
Hi Roger,
I usually use Bluing reagent (Richard Allan Scientific, part of Fisher)
after Hematoxylin and tap water, then wash again before eosin. Try it
and you will see big difference. I just love it!
All the best,
Naira
--
Almost any method will work in intestine. I have had good results with Holmes'
stain and more impressive results with Kiernan's method. I have also had good
results with Winkelmann's method, but it is terribly time consuming.
-Original Message-
From:
When I have used tap water, I use cold running water for 5 minutes.
Works fairly well, depending on the pH of your tap water.
Eva Permaul wrote:
Good morning,
I was wondering if someone uses tap water to blue their slides after
Hematoxyline. If yes, do you use warm or cold water and for how
Hello Histonetters!
We are in the process of purchasing a PATHOS Microwave Histoprocessor.
What kind of experience did you have? How long were fixation/processing
time in general? How happy were you with the results?
We are a research lab and process mainly animal tissue.
Any feedback
Dear all,
I am using a Technovit 9100 PMMA embeding system and the pre-polymerisation
step requires a partial vaccum. Partial is everyting which is not total
100% (Absolute) so the rage is quite wide.
Does any one using Technovit 9100 could tell which poucentage of vaccum to
apply to the
We are interested in purchasing an automatic stainer (and possible
coverslipper). The Leica ST5020 model was recommended to us. Is anyone
familiar with this stainer and/or Leica's CV5030 robotic coverslipper? The
price for both is quite high and I want to make sure people are satisfied with
it.
I don't think you can blue too much.
If the pH is too high then it can bleach the haematoxylin.
Any mildly alkaline solution will do (in fact neutral tap water (pH 7)
will slowly get there.
The special blueing solutions available are many and varied:
Warm tap water, phosphate buffer (pH7-8), a
Hello Histonetter,
I hope someone out there in
histoland can help me. I am about to be thrust (kicking and screaming)
into the world of undecalcified bone histology and I need lots of help. My
goals for 2009 will involve sectioning/staining and doing Image
Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are
looking into changing to commercial from our ones made in house, so any help in
deciding which ones to trial would be appreciated.
Tanya
LabPLUS
Auckland City Hospital
New Zealand
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