Questions about xylene substitutes: This has been discussed a good
many times on Histonet.
There are two classes of xylene substitutes:
Limonene and turpentine are terpenes. Several brands; AmeriClear was
mentioned. Limonene is prepared by steam distillation of orange peels.
It has a strong citru
All-
I did say the unit was a real workhorse! In all honesty, we did use ours hard
for well over 10 years before we moved on the the newer VIP 2000s and VIP 3000s.
Unfortunately, we remember our last experiences better than the early ones. We
had many good years with our VIP 1000s. It wasn't
I just researched this recently for procedure writing. I referenced an article
from the CDC that made mention of specific handling guidelines for histology,
and also specifically the formic acid treatment step. I do not have the article
in front of me, but by my recollection I believe that it s
One time we tried FISH on a cellsuspension dropped on a superfrost slide. It
was airdried. In the fluorescence microscope the nuclei were surrounded with
strong fluorescence. In another trial we heated the slides before FISH and
the slide was more or less clear.
I concluded, that the reagens on the
Has anyone using Starfrost slides (7255) from Mercedes had any problems with
autofluoresence when doing IF?
Thanks in advance.
Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit
==
C
Hi,
I was reading your email of Jan. 15/09 on the subject of "Prion
Contamination". In it you had made the following statement, which I was
curious as to where this information had come from.
"the procedure to inactivate using formic acid is followed before
fixation and processing. If the tissue i
We participated in the CAP teleconference in December. The CAP
recommendation is to use 1 of the G codes per case and that it does not
matter how many jars are submitted, just how many cores. (Yes, this is
different than the standard 1 jar/ 1 billing code policy).
Lester J. Raff, MD
Medical Direc
Hello Histonetters,
With the new Medicare G codes for saturation biopsies of the prostate, the
flyer reads:
G0416, Surgical pathology gross and microscopic exam for prostate needle
saturation biopsy sampling, 1 to 20 specimens.
G0417, 21 to 40 specimens.
G0418, 41 to 60 specimens.
G0419, greate
Look in Freida Carson's second edition, page 80.
Stock Giemsa is one yr
Stock silver nitrate for GMS is one month
Andi Grantham
At 09:25 AM 1/22/2009, Nancy Schmitt wrote:
Help!
We have a student in the histo program. She is making stock
solution for silver stain and Giemsa stain. How lon
Kim,
We also use the Basalcellcocktail (with p63).
The protocol is put together of the two single protocols for the single
markers with the usual incubation times. The first HIER step is naturally
the retrieval for both antigens. The first part is the dab-kit. After the
colour-development a second
We use both Propar and Formula 83. Propar works well for the most part
but does have a tendency if it's humid, to dry moisture out of the air
as we have seen eosin bleed from time to time with it. It doesn't have
much of an odor either. Formula 83 works wonderfully but it smells like
ether! We
I have been servicing the VIP-1000/2000/3000 (aka: "K" Series) tissue
processors for over 15 years. They were manufactured new from approximately
1983 to 1993. They were manufactured and private labeled for Miles
Scientific, Inc. by Sakura Finetek. In the time Miles sold them new, I
estimate that
Albany Memorial Hospital
Albany, NY
Full time Histotechnician position available 6:30 am to 2:30 pm, Mon - Friday,
no weekends.
Must be NYS licensed and have ASCP certification.
Responsibilities include, routine histology, IHC, ISH, special stains and
frozen sectioning.
If interested please subm
Paula-
It's a hard problem to diagnose with out knowing what platform you are using,
but here is what I have learned from our experience-
- We had the same trouble on our Ventana Benchmark and after much hair pulling
and troubleshooting, found it to be the slides we were using. The only slides
Help!
We have a student in the histo program. She is making stock solution for
silver stain and Giemsa stain. How long are stock solutions good for? We are
not finding that info.
Thanks
Nancy Schmitt
Dubuque, IA
NOTICE: This email may contain legally privileged information. The informat
Hi Jennifer,
we perform the doublestain routinly. The preferred protocol is CK HMW/p504s
on prostata biopsies.
The protocol is easy to establish and the staining results are very good.
The run lasts about 4 hours and we do it overnight. You need the Red and the
DAB ultraview kit. No extra reagens.
Do you make the silversolution with ammonia? If the silversolution isn't
properly retitered with silvernitrat, there is an excess amount of ammonia,
that makes a yellowish background.
The silversolution has to be faintly grey.
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.ut
Hi histoneters,
Please help me to find an Ab to detect keratin 18 in mouse tissue, this
means that at has to be not mouse Ab.
Thanks in advance,
Naira
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/ma
Hello colleagues of the tissue world!
I am working on developing (revising, actually) up-dated standards for CLIA
proficiency testing in my lab. We are only doing Histochemistry, Immuno
fluorescence for dystrophin and dystrophin assoicate proteins and
Immunofluorescence for both kidney and card
Suggestions:
1) If it's compatible with the autostainer that you're using, add a
surfactant such as Tween 20 (0.05%) to your buffer rinse solution, in order
to cut the surface tension and allow the staining reagents to flow across
your slides more evenly.
2) If it's possible, add more volu
We use XS xylene substitute and we get it from Statlab. No odor.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
3 Children's WaySlot 820
Little Rock, AR 72202
phone 501.364.4240
fax501.364.3155
visit us on the web at:www.archildre
Not much help can you get if you do not say which autostainer you are having
the problems with, because not all work in the same way.
René J.
--- On Wed, 1/21/09, Paula Lucas wrote:
From: Paula Lucas
Subject: [Histonet] Auto-IHC Staining Issues
To: "histonet@lists.utsouthwestern.edu"
Date: We
The VIP 1000 beats all other hands down. Get a demo to be convinced.
René J.
--- On Wed, 1/21/09, Atoska Gentry wrote:
From: Atoska Gentry
Subject: [Histonet] Re: tissue processors
To: "Histonet"
Date: Wednesday, January 21, 2009, 5:30 PM
hello, we are in the market for a new/replacement tiss
Tom:
Dehydrate with graded isopropyl alcohol and clear with mixtures 5:1 and 2:1 of
isopropyl and mineral oil (at 50ºC), followed by pure mineral oil (50ºC) and
your regular infiltration with paraffin.
You will eliminate xylene totally and the used mineral oil will be disposed off
mixed with the
We are a small hospital lab and are beginning to do some prostate
biopsies. There will generally be 12 specimens per case. Do we bill
88305 for each one (12) or is there some kind of "bundling" of charges
for these? Thanks for any input. Jane
Jane Moose
LIS Coordinator
Newberry County M
Tom,
We are using Formula 83 from CBG and like it a lot.
Linda
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lbla...@digestivespecialists.com
-Original Message-
From: histonet-boun...@l
Dear Lynette,
Yes we do, in fact our Clini-RF Rapid Freezer goes lower in temperature
to -80 deg. C. Plus there are
a range of options available.
Best Regards
Alan Bright
Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England
Tel No:+44 (0)1480 454528
Fax No:+44
Wondering what folks are using as a xylene substitute. We have used Americlear
for many years and it has worked well. I would like to find something that is
biodegradable and doesn't have such a strong odor. Any suggestions or
recommendations? Thanks.
Tom McNemar, HT(ASCP)
Histology Co-ord
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