Try a Papanicolau stain.
René J.
--- On Thu, 3/5/09, Cheryl Crowder ccrow...@vetmed.lsu.edu wrote:
From: Cheryl Crowder ccrow...@vetmed.lsu.edu
Subject: [Histonet] Epithelial cells
To: Histonet histo...@pathology.swmed.edu, Histonet
histo...@pathology.swmed.edu,
hi, i dont have experience on vibratome section at 50 um (for electrophysiology
we cut 300).
but I performed a lot on microtome or cryostat - frozen sections at 20-40 um.
if you want the brain to be harder, perfuse longer and have better
post-fixation.
unless your antigen is more than fragile
Hi,
I use Invitrogen's rabbit (A11122) GFP at 1:1000.
Paula Pierce, BS, HTL(ASCP)HT
Excalibur Pathology, Inc.
631 N Broadway Ave
Moore, OK 73160
405-759-3953
www.excaliburpathology.com
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another option is the antibody from NeoMarkers/Labvision MS-1288. I have
used for several years mostly in mouse model but also works in other
species and cell lines. have reference if you like + feel free if you
have any questions
-Original Message-
From:
Dear Sightdog(?),
You did not give your real name (Sightdog doesn't count, unless that IS
your real name). You also did not indicate what facility you are with. I'm
sure you are on the up and up, but HistoNet Netiquette requires us to
identify ourselves and readers would like to know who we are
I know this lab - they are very much legitimate.
What lab?
Jackie M O'Connor wrote:
I know this lab - they are very much legitimate. I would suggest anyone
interested in the equipment contact them at the email provided, and they
will be happy to talk terms and negotiate price one-on-one.
Just wonder anyone has a suggestion of antibody that works on rat brain? Frozen
section and parafin sections?
It would be great if this also works on mice.
2009-03-06
TF
发件人： Colleen Forster
发送时间： 2009-03-06 00:57:29
收件人： tifei
抄送： iskaliora; histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ
主题： Re:
We have had success staining murine paraffin sections using Rockland goat
anti-GFP antibody.
You'll need to optimize it to your laboratory conditions trying with either
antigen retrieval (citrate buffer pH 6.0) or Proteinase K. Make sure you have
tissue with known positive GFP expression and a
For mouse (specifically) have used CD31 from BD catalog number 550274. Its a
rat ant-mouse (Clone MEC13.3).
can supply more info if need.
The problem I have found is finding good control material for this marker.
especially since my only source tends to be the investigators that provide me
the
This is a resend of a previous message that seems to have been chopped
to pieces in cyberspace.
Hope this one comes through better:
I prefer to use iron gallein elastin stain for demonstrating
elasti fibers. It is simple to make up and gives good contrast
compared to some
I would guess that there are problems with the antibodies you are using. SMA is
usually a very robust antigen for IHC, if you're not getting any staining
you're probably using the wrong antibody. You need an antibody whose specs
indicate that it works on mouse, and that it works on fixed
Hi,
I need to work on zebrafish for the first time and would like to get some
general protocols for fixation and IHC staining.
My investigator used 4% PFA for 2 hrs at RT for a whole zebrafish and
sections didn't come out right (falling off not preserved well).
I am going to increase the time and
I have posted before, but would like some more feed back on this issue. Thanks
to everyone who has already given me feedback on this! I would like to start
working a person on third shift to try and relieve some stress for 1st shift.
Is there any one who would like to give me their thoughts
Have you checked out the ton of protocols (including histo) available
at ZFIN online:
http://zfin.org/zf_info/zfbook/zfbk.html
Hi,
I need to work on zebrafish for the first time and would like to get some
general protocols for fixation and IHC staining.
My investigator used 4% PFA for 2 hrs
Hi MaryAnn,
I am also about to take my HTL exam. The website has all the information you
are looking for. I've been through these testing facilities when I took my
GRE. Make sure you have at least 2 forms of I.D. with you, do not bring a
cell phone, etc (all this is on the ASCP website). You
Hi everyone. Happy Friday!
We are in the process of reviewing our morgue policies, and the question about
ownership has come up. How many of you are responsible for maintaining the
morgue? Some hospitals I know have Security in charge, while others say Nursing
or the Lab. Who is responsible
Dear Histonetters!
I was wondering if there are any or someone knows a veterinary pathologist
who has experience working with mouse tissues and possibly Xenograft models?
The pathologist we were outsourcing to has moved to a different state. We
need one in Boston or nearby Massachusetts area.
Any
MaryAnn,
The ASCP uses Computer Adaptive Testing (CAT) for the certification exams.
As another person responded your questions will be based on the response
to the previous questions. If you get the answer right subsequent
questions asked will be of increasing difficulty and worth more
http://www.ascp.org/pdf/HTHTLSummaryofStainsforComputerExamination.aspx
Sorry. The link was incomplete.
Jennifer MacDonald jmacdon...@mtsac.edu
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/06/2009 01:59 PM
To
MaryAnn Dixon dix...@vetmed.ufl.edu
cc
I am having a problem with Biebrich Scarlet. I am attempting to do a
Masson's stain on some mouse heart tissue. Instead of the strikingly
bright red of the muscle tissue I am getting a purple color instead.
Everything else looks fine. The hearts are fixed in Penfix and are
embedded in paraffin.
Pathology/Histology is responsible for the autopsy suite, providing the
autopsy service, stocking the morgue. Nursing is responsible for the
delivery of patients 24/7 to the morgue. Histology is responsible for
releasing patients on day shift and arranging the autopsy if one is
requested. We
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