Hi All,
Is it Friday yet? I have been ill and my synaptic responses are miss-firing.
Beckman Coulter bought Dako's Flow business unit and in 2007 the Private equity
fund EQT V (“EQT”) signed a definitive agreement to acquire 100% of Dako. The
total consideration for the transaction is DKK 7.2
No offense, I just would rather use the website to seek info, because I do not
have the time to keep up with all of the emails.
Thank you,
Trish Meyers
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Hi All,
Dako was not purchased by Danaher. FYI.. Read posted Milestones from Dako's
website below:
Regards,
Akemi Allison-Tacha BS, HT (ASCP) HTL
President
Phoenix Lab Consulting
Specializing in Histology, SS, IHC, & TMA
Tele: (425) 941-4287
E-Mail: akemiat3...@yahoo.com
Corporate Milestones
Hi Meghan,
We had the same problem with Vector VIP - I stain both atherosclerotic
specimens and burns - for some reason the athero samples were fine but the
burns compeltely lost their colour. It turned out, for us, it was the
alcohol in the dehydration step that was stripping the colour. I would
Can anyone say AIG/Freddie Mac/Fannie Mae?
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Thomas Jasper
Sent: Thu 3/12/2009 4:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Dako
I also believe that competition is good for the
OK,
There've been plenty of shots taken at Dako...and from what I can tell
rightly so. I agree with the statements about the "good old days" and
all, and I would still like to believe Dako is a decent antibody
company.
So here's my question - How 'bout it Dako? Are you going to take all of
th
My colleague and I both love BioCare products (and personnel). She also
has recently gotten the Intellipath immunotainer and loves it. I also
like Vector products and their tech support. Just my two cents!!
D
The information transmitted is intended only for the person(s)or entity to
which
OK that's IT! I'm going to start using my spellchecker!
:)
Friday seems early this week, at least on this forum!
Bill
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
O'Donnell, Bill
Sent: Thursday, March
I would like to ask how any of you feel about the Dako's Flex system. I'm
having a hard time liking a system that is supposed to be a strong polymer, but
needs a higher pH retrieval step to work. I also find myself tittering
antibodies to a lesser degree.
I should explain I
Actually, one of their greatest assets in the early days was their
customer service and the information they were able to offer on such a
new technology. I had many students (OJT)practically that gray
immunoperopxidase book (can't recall the name)they used to hand out.
Five years ago, when we went
I just wanted to thank everyone for not buzzing me about all my typos in
the post! Man, that was bad!
William (Bill) O'Donnell, HT (ASCP) QIHC
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847
-Original Message-
From: Thomas Pier [mailto:t...@medicine.wisc.
Claire,
One of the things that irritates me about some companies is their tendency to
charge for their products based solely on what we get reimbursed rather than
being happy with making a healthy profit based on their expenses.
Doug Showers, MS, HT
Histology Manager
-Original Message-
Why do I keep feeling more and more like these guys are the 'contractors', and
we are the 'government'. i.e. $50 screwdrivers come to mind... I think we need
to start looking into these guys like others are looking into the
pharmaceutical market pricing. They think they have us over a barrel. I
Correction If they did not know the answer they told me and I really
appreciate that. 'Cause some companies and people just keep talking when
they don't know and it does not help. Just wastes time.
Pamela A Marcum
University of Pennsylvania
School of Veterinary Medicine
Comparative Orthopedic
I don't understand why your prices are so different from mine. I just got a
new quote from my Rep and my price increases were very reasonable. There seems
to be a large discrepancy in prices across the USA. I would call the office at
DAKO and complain or like you said change vendors. I would
Thanks for the heads up!
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Weems, Joyce
Sent: Thu 3/12/2009 10:53 AM
To: Burton, Lynn; Pamela Marcum; Victor Tobias; jeff
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Dako, Thermo
DAKO reps used to be great, it is a pity. We have not been contacted in mnay
years either. I used to learn so much from them back when I was newer to the
field.
-Original Message-
From: "O'Donnell, Bill"
Date: Thu, 12 Mar 2009 08:58:30
To: jeff;
Subject: RE: [Histonet] Dako
Hav
Hi,
First, I DO work for BioCare Medical on the ea= st coast and,
Godsgalnow, you are right. Joe Myers is a great guy, very hel= pful
and knowledgable. I'm not an HT, but I sure have learned a LOT from
hi= m. Just about everyone that knows him thinks he's great.
DIDO They have been great with me and I do some really strange animal
stuff. Innovex has always helped or if they did know said so, which I
really appreciate.
Pamela A Marcum
University of Pennsylvania
School of Veterinary Medicine
Comparative Orthopedic Laboratory (CORL)
382 W Street Rd
Kenn
Dako has lost their way. Customer service and affordability has suffered badly
since the buyout. Luckily, there are always other vendors...and this is coming
from a Dako fanatic.
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
___
Histon
What does their DAB+ cost now?? I love it and find it to be most reliable but
find Zymed's DAB+ to be just as good (I think Zymed may be a part of Invitrogen
now?).
-Original Message-
From: Thomas Pier
Date: Thu, 12 Mar 2009 10:08:00
To: ;
Subject: Re: [Histonet] Dako
No, but the p
Paul,
For MMA embedded specimens (MMA +DBP), I first deplastify my sections, hydrate
to water, stain in 5% silver nitrate solution for 5 minutes (in the dark), wash
times three changes in DI H2O (in the dark), develop in sodium-carbonate
formaldehyde solution for 2 minutes (in the dark), wa
Greetings everyone ..
I think this may have been mentioned on this list once before in 2005
(judging from the Archives), but I would appreciate your input. Do other
people use clone AC-1A1 of Factor XIIIa? .. That's the antibody we use here
and I am not particularly comfortable with this antibody
We've been told that we can't get any pricing except for list price unless
we sign a contract. Our purchasing department asked me to start shopping
around for replacement antibodies. In fact, I'm looking for a replacement
for their SMA antibody. Looks like I'm going with AbSerotec for this
antib
Does your facility require bone from a total hip or knee replacements
from patients with osteoarthritis or Degenerative Joint Disease to be
sent to pathology, or are these samples exempt? If they are sent to
pathology, do they perform a gross only or gross+micro.
Thank You,
Dorothy Webb, HT
My favorite is Betazoid DAB
-Original Message-
From: Thomas Pier
To: godsgal...@aol.com; hor...@archildrens.org; anne.le...@bms.com;
billodonn...@catholichealth.net; histonet@lists.utsouthwestern.edu;
jlhow...@yrmc.org
Sent: Thu, 12 Mar 2009 2:21 pm
Subject: RE: [Histonet] Dako
I've
I've had great luck with Biocare as well. They've been my primary supplier for
detection kits and ancillaries since 2003. I'm probably going to check them
out for my DAB needs due to Dako DAB+ going from $170 to $400 for 110 mL.
Tom Pier
>>> "O'Donnell, Bill" 03/12/09 12:56 PM >>>
That's who
Plus, they have Joe Meyer!? He can come and give workshops at your facility for
which you can get CEUs for, as he has been approved by NSH as a provider.
-Original Message-
From: O'Donnell, Bill
To: godsgal...@aol.com; hor...@archildrens.org; anne.le...@bms.com;
histonet@lists.utsouthw
Well, to be fair, worldwide sales conferences have to be somewhere.
'Tis true it 'tis almost Friday, and a wee bit longer til' St. Patty's
William (Bill) O'Donnell, HT (ASCP) QIHC
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847
-Original Message-
From: h
That's who I went with as well. At first I wrote them off because of all
the star trek references, but products are not notch and customer
service is great. Prices are still in the easonable" range, though, as a
result of the recession I might expect a price jump. Even if their
detection kits dooub
To all:
Sorry to bother the list with a question that has no doubt been addressed in the
past, but now that we are looking into possibly replacing our tissue processor,
I would like some input. It no longer is covered by a service contract and
things are starting to go wrong with it. We have a
Innovex is a wonderful company that offers good products at an extremely
reasonable cost and provides exceptional tech support.
No, I don't work for them...I've just had great experiences.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.uts
Someone has to pay for their yearly world wide sales meetings...Dako just had
theirs in South Africa...imagine what that cost!
>>> On 3/12/2009 at 10:41 AM, "jeff" wrote:
I was wondering if anyone else is being blackmailed by Dako to sign a contract
with them. We have enjoyed our relationship
HI Laurie,
Have you tried cutting only one section per slide & fixing it immediately,
allowing it no time to air dry. This is our practice we place only one
section per slide & place the slide immediately in fixative. The
pathologist have become extremely particular about this step. You may be
gett
LOL! It's ALMOST Friday, people, ALMOST... :-)
--On Thursday, March 12, 2009 11:46 AM -0500 Jackie M O'Connor
wrote:
Not Dako - Leica purchased Surgipath.
"Burton, Lynn"
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/12/2009 10:46 AM
To
"Pamela Marcum" , "Victor Tobias"
, "je
Not Dako - Leica purchased Surgipath.
"Burton, Lynn"
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/12/2009 10:46 AM
To
"Pamela Marcum" , "Victor Tobias"
, "jeff"
cc
histonet@lists.utsouthwestern.edu
Subject
RE: [Histonet] Dako, Thermo and others
Dako did acquire Surgipath re
All of you fed up with DAKO and thermo et al.I figured that out along time
ago and switched to a new company called Biocare.? And now I have been using
them for years.? They still offer that personal touch.? They still do reagent
rental agreements.? They still offer discounts based on purcha
Danaher owns them both.
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!
My purchasing agent notified me of the huge markup of their
reagents/antibodies. I called my rep told them I was unhappy and would
have to look elsewhere and they decided to give me the old pricing for a
year. I still don't have it in writing.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of
I believe it was about a year ago that the family that started Dako finally
sold it. The "new powers that be" have since raised prices 3 to 4 times.
Luckily I do not need very many of their products and am now trying to avoid
them, as well as other companies that have gobbled up smaller companie
I believe that that is because of the thickness of the sections and the fact
that they are in plastic. Also if the plastic is GMA you cannot remove it and
so therefore it takes longer for it to penetrate.
Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopae
That's correct ... Leica = Surgipath
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Thursday, March 12, 2009 8:53 AM
To: Burton, Lynn; Pamela Marcum; Victor Tobias; jeff
Cc: histonet@list
Leica bought Surgipath, not Dako!
This e-mail and any files transmitted with it are confidential and are intended
solely for the use of the individual or entity to whom they are addressed. If
you are not the intended recipient or the individual respons
I'm pretty sure Danaher owns Surgipath now.along with nearly everything
else.
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
No trees were hurt in the sending of thi
I wasn't aware that Thermo now has the rights for the PSLIM. Thanks for posting
this Victor. I did have an interest in this printer at one time, but based on
this information I won't be considering it.
Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Caro
Leica acquired Surgipath, not DAKO... j
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton,
Lynn
Sent: Thursday, March 12, 2009 11:46 AM
To: Pamela Marcum; Victor Tobias; jeff
Cc: histonet@lists.utsouthw
Our Von Kossa Procedure calls for placing the slides in Silver Nitrate Solution
for 2 minutes in the dark, rinse in distilled water, develop in Hydroquinone
Solution for 1 minute, fix in Sodium Thiosulfate, Rinse, counterstain with Acid
Fuchsin rinse in 1% Glacial Acetic Acid Solution quickly de
Dako did acquire Surgipath recently. The Surgipath rep said it has been a good
"marriage" for them so far.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Pamela Marcum
Sent: Thu 3/12/
Does anyone know any good antibody against human NFS1 (nitrogen-fixation 1
for bacteria, cysteine desulfurase for human) for cell staining?
Thank you in advance
Suh
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Hey Everyone
I am looking for a microwave processing protocol for large derm cases, such as
excisional melanoma cases.
Any thoughts?
Looking for process to include fixation, and fixative used
Subsequent processing steps, to include solutions used, time and temperature
And grossing tip
AMEN
Pamela A Marcum
University of Pennsylvania
School of Veterinary Medicine
Comparative Orthopedic Laboratory (CORL)
382 W Street Rd
Kennett Square PA 19438
610-925-6278
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwester
The standard Von Kossa silver stain for calcium calls for 20 minutes in the
silver nitrate solution under UV light. There is a modified Von Kossa for
plastic embedded bone sections, which is identical except it calls for a
minimum of 6 hours in the silver nitrate solution under UV. Does anyone
We not only have noticed a huge mark-up on their products, but it is taking
longer to receive shipments from them. Did Dako go through some sort of re-org
or had major lay-offs resulting in fewer people having to do more??
Anne
>-Original Message-
>From: histonet-boun...@lists.utsouthwe
No, but the price of their DAB+ did jump drastically. I like the product, but
I may have to find an alternative.
Tom Pier
>>> "jeff" 03/12/09 9:47 AM >>>
I was wondering if anyone else is being blackmailed by Dako to sign a contract
with them. We have enjoyed our relationship a number of year
Just exactly that!
René J.
--- On Thu, 3/12/09, Emily Sours wrote:
From: Emily Sours
Subject: Re: [Histonet] silly question about goat serum
To: histonet@lists.utsouthwestern.edu
Date: Thursday, March 12, 2009, 10:41 AM
What exactly does non-immune goat serum mean? The goat hasn't been exposed
Ah the wonders of capitalism and the offer-demand paradigm!
For blackmail to take place, as to tango, it takes two.
If they behave that way, get another provider. Perhaps if many just to that,
they will start to behave as they used to be, friendly and accommodating.
It most be the result of a new
Jeff,
It is not just Dako, but many vendors have jacked up the cost of goods.
Let's take the PSLIM from Accuplace. About a month ago you could
purchase this for $5K. Now that Thermo has acquired distribution rights
it costs over $10K and nothing has changed. To me, it is better to sell
10 at
Haven't had it happen yet, but I have been systematically firing them
for a number of reasons, not the least of which is no contact with any
sales reps by their initialization for close to 8 YEARS! (Except when I
complained about this and they showed up unexpectedly on my day off, so
it doesn't c
Hi Emily,
Normal as opposed to immune serum. If a goat outside of a "Specific
Pathogen Free (SPF)" facility were to actually have no Ab's it would be
far from normal! I'm picturing the Boy in a Bubble episode on Seinfeld-
only substitute one kid for another!! LOL (or groan if you prefer).
Enjoy the
Hello all,
What kit(s) are people using nowadays to biotinylate their own antibodies? I
looked online and saw numerous kits available. I figured I'd ask my peers to
see if their was a quick and easy kit out there.
Any info, would be helpful.
Thank you.
Gustave
Gustave T. Hebert
Rese
What exactly does non-immune goat serum mean? The goat hasn't been exposed
to any specific antigen, therefore it's normal?
Emily
--
prometheus, thief of light, giver of light, bound by the gods, must have
been a book.
-mark danielewski, house of leaves
I was wondering if anyone else is being blackmailed by Dako to sign a contract
with them. We have enjoyed our relationship a number of years buying there
product as we need it. We also have changed detection kits as we where advised.
Now they are telling us that if we do not go with price per s
Paula,
Try changing your microtome blade. Get as many samples as you can from the
companies and try them all out. We see a difference in sectioning paraffin
blocks just by changing blades. You'll find something that works well for your
paraffin and room ambient conditions. If your room is warm,
Yest, it is.
René J.
--- On Thu, 3/12/09, Eva Permaul wrote:
From: Eva Permaul
Subject: [Histonet] silly question about goat serum
To: histonet@lists.utsouthwestern.edu
Date: Thursday, March 12, 2009, 9:22 AM
Good morning,
I have a question that is probably very silly but I just don't want to
Good morning,
I have a question that is probably very silly but I just don't want to
make any mistakes with the experiment I am suppose to do. I am used to
the protein blocking being called normal goat serum (10%). The kit I am
about to use says their's is 10% goat non-immune serum. Is this the
Kris,
If you want to melt paraffin for changing the processor, etc. you might
think about getting a paraffin dispenser and a separate slide dryer.
We have lab heat issues in the summer. We got rid of the incubator that
melted our paraffin and got a Paraffin dispenser (TBS cat # PD-120). It
holds
Some of my histotechs used to do that but just the instant before taking the
section, after all the rimming was done. They had good results using the same
spray used for doing FS.
René J.
--- On Thu, 3/12/09, Breckenridge, Sue wrote:
From: Breckenridge, Sue
Subject: [Histonet] chilling paraff
Breckenridge, Sue would like to recall the message, "chilling paraffin blocks
question".
Confidentiality Notice: This e-mail message, including any attachments, from
Cape Regional Health System contains information which is CONFIDENTIAL AND/OR
LEGALLY PRIVILEGED. The information is intended onl
Wondering what are your thoughts on chilling paraffin blocks via a
tissue freezing spray (e.g. ones used to further chill frozen sections)?
Thanks,
Sue Breckenridge, Histology Supervisor BS, HT/HTL(ASCP)
Cape Regional Medical Center
Cape May Court House, NJ
Confidentiality Notice: T
Wondering what are your thoughts on chilling paraffin blocks via a
tissue freezing spray (e.g. ones used to further chill frozen sections)?
Thanks,
Sue Breckenridge, Histology Supervisor BS, HT/HTL(ASCP)
Cape Regional Medical Center
Cape May Court House, NJ
Confidentiality Notice: This
Good morning everyone! Our Tissue Tek VIP 5 stopped working last night
and I am waiting for someone at Sakura (Pacific time) to call me(Eastern
time). Does anyone have a program for processing small biopsies
manually? I searched the archives and was unable to find anything. I
would appreciate any h
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