Hi Ana,
Yes, I work for an aquaculture veterinary pathology lab and have experience
with some of these, but particularly fish.
Please feel free to contact me for more information, I'll help if I can.
Debbie Faichney
Histopathology
Institute of aquaculture
University of Stirling
Stirling
Hello Histonetters,
I understand most of the professionals on this website are histology
professionals, however, I thought I would give it a try since I have not
found a cytology listserv yet. Does anyone know how many slides per day a
cytotechnologist would screen within a private lab setting (On
Updated request with model number.
Does anyone have a copy of the Tissue-Tek II manual that they would be
willing to share? (PDF preferred, hard-copy graciously accepted)
One for a model 4553 Cryostat (apologies for not including that in the
original post)
Thanks (in advance),
Bernie
Terri:
Any private setting (including large reference labs) is ruled by the same
standards as any other one. The screener threshold (when you need to hire an
additional one) is 7,000 slides/year.
Under separate cover I am sending you an article I wrote about the Cytology
work within the
Hi all,
Can anyone suggest a evolutionary conserved keratinocyte marker? I'm
working with a fish (not zebrafish!) and need an antibody marker that
can ID keratinocytes.
Thanks
--
Tyrone Genade
http://tgenade.freeshell.org
email: tgen...@freeshell.org
tel: +27-84-632-1925 (c)
Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
After we cut the brain sections on a cryostat (OCT embedded), we brush a drop
of 0.1M PB on to the slide before mounting the sections.
Do anyone know the side effect of this - for example, the sections will peel
off during immunostaining?
Another question is about the dry time after mounting
We recently purchased a Dako Autostainer with the PT Link for our IHC's. We
are experiencing more background staining then before when we had the older
version of the Dako Autostainer. Does anybody use protein block (serum free)
to help minimize background staining? Thank you for your replies.
The circles could be either from air bubbles present when you mount the slides
onto the coverplates, or they could have arisen after exposure to H2O2 as part
of the staining procedure. Doing the H2O2 off instrument in a coplin jar and
then rinsing and mounting them in the coverplates will help
I have a license to drive a car, and a certification that allows me to
pay each year to ASCP. I feel so credentialed! I think we should all
take Saturday and Sunday off.
Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Laboratory Mgr
One Medimmune Way, Lab 2438-Area 4
Gaithersburg, MD 20878
Fellow Histonetters,
I am having an extremely hard time obtaining tonsil control tissue and was
wondering if anyone would have some they could share. I would be glad to
exchange some rhabdomyosarcoma tissue blocks in exchange. Please let me know if
you can help.
Thanks,
Charlene Henry HT
Contact me off line and I'd be happy to see what we can do to
accommodate you at jrobert...@pathologysciences.com
Thank you.
Jodie Robertson, HT (ASCP) QIHC
Pathology Sciences Medical Group
Histology Day Supervisor
183 E. 8th Ave.
Chico, CA 95926
530-891-6244
-Original Message-
From:
Someone gave me some small PMMA blocks polymerized inside glass scintillation
vials. I always use plastic vials. Obviously the vials have to be broken to
get the blocks out. Does anyone have some advice on the best way to do this?
The guy who brought them said he thought I could use liquid
Hi Paul,
I did so many of those PMMA blocks cracking. Take a under pad, line your
blocks not too many and cover with another under pad and start
(literally) hammering them one by one with hammer gently I am not
kidding. Make sure you wear safety glasses and double gloves to protect
your hands.
I agree. It is a little messy and can be dangerous if you are not paying
enough attention. LN just gets the glass cold and cracks it so you don't need
a hammer. If you leave it in the LN too long the block may crack with the
vial.
Pam Marcum
UPENN Vet School
New Bolton Center
I need help! Just received a needle biopsy of liver submitted in normal
saline. They did all the routine cultures/ fungal, and are requesting a
wet mount on this tissue for Entamoeba Trophozoites.
I have contacted all of my resources in and out of state, and they
cannot help me.
HELP!!
Paul,
Put in LN2 (or leave in -80 freezer for about 1 hour). Using the appropriate
PPE, wrap in towel, hit with Hammer (gently...), welcome to use this SOP.
Bob,
Robert Schoonhoven
From: Monfils, Paul pmonf...@lifespan.org
To:
I would not use liquid nitrogen. The safest way to do this is to put the
blocks in a refrigerator freezer for a couple of hours. Remove quickly, remove
caps, wrap in paper towels and hit with a hammer. Be sure to wear protective
clothing, eye covers and gloves. When the glass has broken
Folks,
I need some input on a problem we're seeing with some mouse brain
samples. The samples are from new born mice P0 to P14 which have been
fixed in 10% NBF (Fisher brand and well within expiration date) for 72
hours. They are then transferred to 70% etoh and processed on an 8 hour
process.
This question is specifically for Linda Jenkins or anyone who has worked
with Technovit 7200,
I am trying to do an HE stain on ~70 um thick ground sections on
plastic slides. I have followed the Donath method all the way through
processing, but the sections do not pick up stains the way the
To my knowledge it is near impossible to obtain an adequate HE stain
on thick resin sections. If this is not true, I would be interested in
the same information.
Jack
On Apr 3, 2009, at 6:12 PM, Garcia, Lori, Sr. Scientist lori.gar...@medtronic.com
wrote:
This question is specifically
Anyone else think this Rene fella comes across as a real know-it-all?
From: Rene J Buesa rjbu...@yahoo.com
To: histonet@lists.utsouthwestern.edu;
histonet-boun...@lists.utsouthwestern.edu; Lynette Pavelich
lpave...@hurleymc.com
Sent: Friday, April 3, 2009
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