Re: [Histonet] "FREEZY" spray

Sorry, again, I'm confused... are you responding to me? I'm not the original poster... I never stated I had trouble with cracking, because, well, I don't. I'm Bernie Taupin, the King of Cryomicrotomy, Esq. From: Akemi Allison-Tacha To: Jennifer MacDonald ; In

Re: [Histonet] "FREEZY" spray

Sorry, I too noticed that the text looked weird then I did a cut and paste.  I keep all the Data Sheets I created in my files, and this was just a portion of the Notes from the original Data sheet.  I thought it might be helpful because you stated you had difficulty with cracking.  You did not m

Re: [Histonet] "FREEZY" spray

I use isopentane suspended in liquid nitrogen, too. sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...? From: Akemi Allison-Tacha To: Jennifer MacDonald ; Ingles Claire ; Bernie Taupin Cc:

Re: [Histonet] "FREEZY" spray

Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS.   Years later, I developed the "MATSSE (pH3.4) 1-Step Trichrome Stain Kit" (Modified Trichrome Method for Muscle Biopsies Cut

Re: [Histonet] "FREEZY" spray

> Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut > fatty sections in the cryostat without it! I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? I find that it leaves to many ice crystal artifacts anyw

Re: [Histonet] Whole human feet

No offense, dude, but GROSS. That's probably why nobody has bitten yet, in regards to this query. From: Yak-Nam Wang To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 8:04:54 PM Subject: [Histonet] Whole human feet Hello all, If possible, we

RE: [Histonet] Unsubscribe

Dear Histonetters: It sure is a real shame to see so many familiar names leave the list just due to a little annoyance. Sorry to send yet *another* note about all of this to the whole list, but I wanted to pass on a link my supervisor sent to me when we were having similar real-life crisis in my

Re: [Histonet] Staying...

Yes, Well-done to all of you who have decided to stay! It really separates the wheat from the chaff, as it were. Or the weenies from the real adults, more or less. And even the French-speakers from the non-French-speakers, in the case of Kemlo/Rene. Ha ha. That was a joke. Learn to laugh a littl

Re: [Histonet] Fixation question - Cerebellar granular cells

Hi, i normally work on tissue. I think 4% PFA in 0.1M PB is fine. so approach 3. 2009-04-08 TF 发件人： Guillermo Palchik 发送时间： 2009-04-08 05:03:13 收件人： histonet 抄送： 主题： [Histonet] Fixation question - Cerebellar granular cells Dear Histoneters, I am doing some IHC on rat cerebellar g

Re: [Histonet] Recycled formalin

Yes. ?? Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>

RE: [Histonet] AE1/AE3 nuclear staining

Possibly the concentration of hydrogen peroxide in the DAB solution has been slowly increasing (maybe micropipette creep?) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmea

RE: [Histonet] gi microwave processing

Nancy, Best advice I can offer is to ensure as much fixation as you can. We start with 30 minutes at 30oC and the ramp it up to 40oC for 30-60 minutes, rinse in 70% ethanol (5min)then continue microwave processing with isopropanol and wax. You may also consider decreasing the processing temperatur

RE: [Histonet] Unsubscribe

Sophie, I for one would never throw a torrent of abuse at any one (mmm unless they are politicians - in between football seasons!) So if my (and most others on Histonet) comments are not of some worth then we apologise. We need to try harder. (also please remember the delete key, I unfortunatel

[Histonet] Recycled formalin

Is anyone using recycled formalin for primary fixation of either surgical or autopsy tissue? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860)

[Histonet] Fixation question - Cerebellar granular cells

Dear Histoneters, I am doing some IHC on rat cerebellar granular cells (NOT TISSUE) and I need to fix them with PF. I have gotten 4 different answers on how to go about this, and I wanted to run this by the list to see what you think. 1) Fix in COLD, 4% PF in 1x PBS 2) Fix in COLD, 4% PF i

[Histonet] Controls needed!

We are looking for H. Pylori control tissue and also GMS/fungus control tissue. Is there anyone out there that might have extra to share? We have good GRAM control blocks that we would be happy to exchange. Please let me know if you can help us out. Thank you! Deanne Knutson Anatomic Patho

[Histonet] RE: I'm outta here

I can spot spam and o.t. posts and delete them in a half second each. The questions on Histonet make me aware of the extent of use of special stains, and the potential for new stains. Jjob postings are forwarded to the career service office here. The safety warnings are often very useful. HI

[Histonet] RE: Tissue Capture Pen

hello, if any of you are using and/or have used the Ted Pella, Inc. Tissue Capture Pen will you be so kind as to share with me your experience (s), pros, cons and whatever else you deem necessary? Also, does it's use require special glass slides? Any info you can provide on it's use ASAP will b

Re: [Histonet] AE1/AE3 nuclear staining

When was the last time you checked the pH of your retrieval solution? Or are you using an enzyme? Mark Tarango On Tue, Apr 7, 2009 at 9:53 AM, Tammy Barnhart wrote: > We have recently been having dark nuclear staining with our AE1/AE3 > antibody. This is a recent event and we cannot figure out

Re: [Histonet] AE1/AE3 nuclear staining

Have you changed any of the reagents in your X-press tissue processor or other aspect of your tissue processing protocol? This could be a factor. René J. --- On Tue, 4/7/09, Tammy Barnhart wrote: From: Tammy Barnhart Subject: [Histonet] AE1/AE3 nuclear staining To: histonet@lists.utsouthwester

AW: [Histonet] MAMMOGLOBIN

I tried also this antibody on Ventana Benchmark. Pity, I had no results. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Vickroy, Jim Gesendet: Dienstag, 07. April 2009 17:54 An: 'Histonet@lis

[Histonet] breaking glass jar/vials for MMA embedded specimens

Yes, the scintillation vials are notorious for shattering in the freezer. However, if you put them in a plastic container with a lid on, the shattered glass will be contained and not all over your freezer. When breaking larger glass containers, again place them in the freezer, remove from the

[Histonet] HI Downdraft fume extractor for sale

I have a HI downdraft fume extractor for $50 and we will provide carbon to refill the drawer. Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailm

Re: [Histonet] Large coverslips?

Hi Yvan, Does it have to be a coverslip? There are some mounting media that harden and form a barrier with optical qualities similar to that of glass. It might not be a perfect solution, but it might work. I think the stuff I used was called Crystal Mount (?). Paula :-) Paula Sicurello V

RE: [Histonet] Paraffin embedded mouse embryos - too dry

Good morning. A colleague of mine recently placed the 10% formalin fixed mouse embryos in 2% agarose, let them solidify, then process at 30 minutes per station under pressure/vacuum. The protocol was published, but right now I don't have that information. Hope this helps in some way! Jen Ander

[Histonet] AE1/AE3 nuclear staining

We have recently been having dark nuclear staining with our AE1/AE3 antibody. This is a recent event and we cannot figure out what is going on. It started gradually and has been increasing in intensity. We have not changed antibody lots or any other part of our protocol. Has anyone seen this be

[Histonet] histonet off-topic comments

Dear Histonet members: I have removed Bernie Taupin from the list. Let's please get back to the topic of histology and related fields.Thanks Linda M Histonet administrator >>> MKing 4/7/2009 8:43 AM >>> a motion is hereby offered to have the active members of histonet vote this troll off the is

[Histonet] unsubscribe

Cynthia Keith HT(ASCP) Histology Supervisor NorDx 102 Campus drive Scarborough, ME 04074 Tel: [207] 885-7907 Fax:[207] 885-7538 Email:kei...@mmc.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the use of the intended recipient(s) only and may contain informa

[Histonet] MAMMOGLOBIN

Has anyone used the DAKO predilute antibody, Mammoglobin, on the Ventana Benchmark XT? And if so.could you share the protocol you used? Finally looking on the Dako website it appears that they have ready to use, could you share with me which antibody should be used with the Ventana I-vie

RE: [Histonet] Nice one Kemlo

I for one would be lost with out this group. I need and miss the collaboration of fellow histotechs on a daily basis. Cheri -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of alan taylor Sent: Saturday, April

Re: [Histonet] When sectioning small long bones in PMMA ....

I have always orientated the specimen in an AP view and taken longitudinal/frontal sections. As for parallel or perpendicular, I may not be completely sure of what you mean. Jack On Apr 7, 2009, at 9:48 AM, "Monfils, Paul" wrote: ... such as a mouse femur, do you prefer to orient the bo

[Histonet] Removing PMMA blocks from glass vials - feedback

Thanks to all who offered advice on this topic. I followed the advice given but my experience was a bit different from that which was described, so I thought I would share it and see if anyone else has had a similar experience. I placed the vials in the -20 freezer for an hour, then went back i

Re: [Histonet] When sectioning small long bones in PMMA ....

neither - I like it on the diagonal. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and di

[Histonet] cryoprotection possible-thanks!

Dear Histonetters, I just wanted to say a big "thank you" to all who responded to my cryoprotection request. We managed it to fix the samples with GA and do a sucrose step accourding to your suggestions: it worked! happy eastern to all, Frauke _

RE: [Histonet] Entamoeba Trophozoites

I haven't checked my email for a few days! Yikes, over 150 messages! But I'll offer this anyway, however late. Entamoeba trophs can easily be demonstrated either in dried fixed smears or in paraffin sections by PAS. Their cytoplasm is loaded with glycogen so they stain very dark. __

[Histonet] When sectioning small long bones in PMMA ....

... such as a mouse femur, do you prefer to orient the bone parallel to the knife edge or perpendicular to the knife edge (or diagonal)? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Paraffin embedded mouse embryos - too dry

I sometimes get embryos in the lab for processing and here are the protocols that I use for embryos in these stages: E12.5 - 70% Ethanol - 12 min 95% Ethanol - 12 min 100% Ethanol x 2 - 12 min each Xylene x 2 - 12 min each Paraffin - 30 min Paraffin x 2 - 1 hr each no vacuum or pressure used e

RE: [Histonet] Eosinophilic granulocytes

Good morning: I have good luck by simply using haematoxylin and eosin stainingthe eosinophils pop right out by virtue of their bright pink/red granules! When used in conjunction with nuclear morphology, one can, with confidence, call these cells eosinophils. I have never performed IHC for e

RE: [Histonet] Re: Histonet Digest, Vol 65, Issue 14

I have tried for the past few days to stay out of this. Might I suggest that if there is a person or persons causing anyone discomfort or problems, simply add them to your blocked list in you e-mail program. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histon

[Histonet] Getting rid of pests

Or Bernie Taupin... Even. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileg

[Histonet] FW: Cervical cancer mission in Peru

From: Savaloja, Lynnette C Sent: Thursday, April 02, 2009 11:06 AM To: Webb, Dorothy L Cc: Semerad, Shelly A Subject: FW: Cervical cancer mission in Peru Importance: High Hey Dorothy, Got an old microtome laying around (see below)? Let me know... L ;)

RE: [Histonet] Job opportunity in Cambridge, MA

Try: Apply this rule after message arrives with Bernie Taulin in the senders address Delete it Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy

Re: [Histonet] Large coverslips?

Whenever I needed to prepare projection slides I coverslipped with another regular glass slide, then cleaned up the edges after the mounting medium dried. Low power microscopy was still possible providing the working distance for the objective lens was greater than 1 mm. Bryan Llewellyn ---

[Histonet] Eosinophilic granulocytes

Hi Is anyone of you aware of a marker/method to stain eosinophilic granulocytes in mouse lung tissue which is formalin fixed and paraffin embedded? Thanks Joost Bruijntjes TNO Quality of Life Zeist Holland TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 6

[Histonet] Paraffin embedded mouse embryos - too dry

Dear all, I'm having trouble with my paraffin embedded mouse embryos. We use E12.5 to E15.5 embryos and we embed and cut the whole embryo. When we try to cut, the paraffin sheets are fine, but as soon as we get to the embryo, the tissue disrupts and tears. I also hear rasping or scraping sound

[Histonet] Pricing of blocks

Good Morning everyone! I am opening a Histology Lab in our Dermatology practice, we routinely do approx. 10,000 biopsies a year.  I would love some input from anyone out there that can assisit me with obtaining a fair price for this task.  The doctors are going to be paying me per block. 

[Histonet] Job opportunity in Cambridge, MA

Hello all! We are a small pathology group within a large, Cambridge, MA based organization looking for a histologist. Our main function is to support drug development in a preclinical setting. The work would involve basic histology, IHC, and special staining on rodent tissue. Our ideal cand

RE: [Histonet] Re: Histonet Digest, Vol 65, Issue 14

Can I just say that you are giving him more importance than he's worth and you are perpetuating it? Obviously he must feed off your responses so stop being manipulated; just ignore or delete or make an Outlook rule, as I have, that his e-mails go to trash!! Bernie, Bernie, where are you? I can't s

[Histonet] please unsubscribe

please unsubscribe This e-mail is for the use of the intended recipient(s) only. The information contained in this communication may be confidential, privileged, or protected by copyright, and may be subject to confidentiality agreements. If you are the intended recipient and you do not wis

RE: [Histonet] Re: Histonet Digest, Vol 65, Issue 14

I second it!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of MKing Sent: Tuesday, April 07, 2009 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 14 a

[Histonet] Re: Histonet Digest, Vol 65, Issue 14

a motion is hereby offered to have the active members of histonet vote this troll off the island, or at least request that the list moderator do so. this person has proven to have absolutely nothing of value to contribute to this group, and is only intent on disrupting it. second? --

Re: [Histonet] Large coverslips?

Yvan: You could use a transparent thin polystyrene sheet cut to the size you need and cement it to your slides with the Canada balsam. I just got somewhat confused with what you wrote about "low power 40x - 100x". If you are referring to an objective magnification, a 40x is always considered a "h

[Histonet] Please unsubscribe me

This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received this e-mail in error, any review, use, dissemination, distribution, or copying of this e-mail is strictly prohibite

[Histonet] HT openings

I have 2 full time openings in my lab at this time. One for a supervisory level HT with IHC experience and one for a becnch level tech. Days, no weekends. If interested please call: Steve Crochiere,HT(ASCP) Histology Supervisor LifePath Partners, LLC @ Mercy Medical Center Springfield, MA 01104

[Histonet] gi microwave processing

I am starting a gi lab and using a microwave processor for my first time. Any suggestions? SHUR Wave processor. Thanks, Nancy **A Good Credit Score is 700 or Above. See yours in just 2 easy steps! (http://pr.atwola.com/promoclk/100126575x1221621488x1201450096/aol?redir=http:%2F

[Histonet] Fw: "FREEZY" spray

  I have to agree with Jennifer on the subject of using freezing spray in cryostats containing potentially infectious agents. I have tried to promote the concept of good cryostat hygiene with everyone I train. When I started out in practice I was greeted by  cryostats that have been left full

[Histonet] "FREEZY" spray

I have to agree with Jennifer on the subject of using freezing spray in cryostats containing potentially infectious agents. I have tried to promote the concept of good cryostat hygiene with everyone I train. When I started out in practice I was greeted by  cryostats that have been left full of s

RE: [Histonet] Staying...

Amen! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marla Thomas Sent: Tuesday, April 07, 2009 7:48 AM

[Histonet] Staying...

I am staying on the list-serve. I have learned a lot from people on this list-serve. I have been in the field since 1970 and I too am still learning things. No one has all of the answers, and sometimes there isn't just one answer. I am staying on the list-serve, and if my input can help one pe

[Histonet] Thank you!

Many thanks to those who responded to my requist for manuals on Medite COT20 and Shandon Histocentre 2: I received copies of both manuals. Also thanks to those few people with hands-on experience with the Fisher Histomatic 166A, who were willing to answer my questions. After some repair/cleani

[Histonet] Large coverslips?

Hi all, I’m looking for some kind of a DIY coverslip or a tape, plastic foil… anything usable to cover some large slides (+/- 4.5cm * 15cm = 1.8inch * 5.9inch) containing botanical and zoological sections mounted in Canada Balsam. Does anyone has a (cheap…) solution for this? It doesn’t need t

[Histonet] Unsubscribe

Please can you unsubscribe me from this mailing list. As an inexperienced histologist trying to find my feet I no longer feel like I could ask any questions as I'm sure I would get a torrent of abuse back instead of any helpful comments. Sophie Ainsworth Brighton and Sussex Medical School Med

RE: [Histonet] Histology

I think one of the things that I've learnt by leaving the world of Histology and dabbling (not too well) in the other disciplines is that there is no such thing as exacts. The maximum and minimum times for fixation for tissue depends on the tissue, the species, the temperature, the manufacturer of