I think one of the things that I've learnt by leaving the world of
Histology and dabbling (not too well) in the other disciplines is that
there is no such thing as exacts. The maximum and minimum times for
fixation for tissue depends on the tissue, the species, the temperature,
the manufacturer of
Please can you unsubscribe me from this mailing list. As an
inexperienced histologist trying to find my feet I no longer feel like I
could ask any questions as I'm sure I would get a torrent of abuse back
instead of any helpful comments.
Sophie Ainsworth
Brighton and Sussex Medical School
Hi all,
I’m looking for some kind of a DIY coverslip or a tape, plastic foil… anything
usable to cover some large slides (+/- 4.5cm * 15cm = 1.8inch * 5.9inch)
containing botanical and zoological sections mounted in Canada Balsam.
Does anyone has a (cheap…) solution for this? It doesn’t need
I am staying on the list-serve. I have learned a lot from people on this
list-serve. I have been in the field since 1970 and I too am still learning
things. No one has all of the answers, and sometimes there isn't just one
answer.
I am staying on the list-serve, and if my input can help one
Amen!
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marla
Thomas
Sent: Tuesday, April 07, 2009 7:48
I have to agree with Jennifer on the subject of using freezing spray in
cryostats containing potentially infectious agents. I have tried
to promote the concept of good cryostat hygiene with everyone I train. When I
started out in practice I was greeted by
cryostats that have been left full of
I have 2 full time openings in my lab at this time. One for a supervisory level
HT with IHC experience and one for a becnch level tech. Days, no weekends. If
interested please call:
Steve Crochiere,HT(ASCP)
Histology Supervisor
LifePath Partners, LLC @ Mercy Medical Center
Springfield, MA 01104
a motion is hereby offered to have the active members of histonet vote
this troll off the island, or at least request that the list moderator
do so. this person has proven to have absolutely nothing of value to
contribute to this group, and is only intent on disrupting it.
second?
I second it!!!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of MKing
Sent: Tuesday, April 07, 2009 6:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 14
a
please unsubscribe
This e-mail is for the use of the intended recipient(s) only. The information
contained in this communication may be confidential, privileged, or protected
by copyright, and may be subject to confidentiality agreements. If you are the
intended recipient and you do not
Can I just say that you are giving him more importance than he's worth
and you are perpetuating it? Obviously he must feed off your responses
so stop being manipulated; just ignore or delete or make an Outlook
rule, as I have, that his e-mails go to trash!!
Bernie, Bernie, where are you? I can't
Yvan:
You could use a transparent thin polystyrene sheet cut to the size you need and
cement it to your slides with the Canada balsam.
I just got somewhat confused with what you wrote about low power 40x - 100x.
If you are referring to an objective magnification, a 40x is always considered
a
/prePFONT face=Verdana color=blue size=1SPAN style=FONT-SIZE: 8pt;
COLOR: blue
This e-mail, including any attachments, is meant only for the intended
recipient and may be a confidential communication or a communication privileged
by law. If you received this e-mail in error, any review,
Good Morning everyone!
I am opening a Histology Lab in our Dermatology practice, we routinely do
approx. 10,000 biopsies a year. I would love some input from anyone out there
that can assisit me with obtaining a fair price for this task. The doctors are
going to be paying me per block.
Hello all!
We are a small pathology group within a large, Cambridge, MA based
organization looking for a histologist. Our main function is to support
drug development in a preclinical setting. The work would involve basic
histology, IHC, and special staining on rodent tissue. Our ideal
Dear all,
I'm having trouble with my paraffin embedded mouse embryos. We use E12.5
to E15.5 embryos and we embed and cut the whole embryo. When we try to
cut, the paraffin sheets are fine, but as soon as we get to the embryo,
the tissue disrupts and tears. I also hear rasping or scraping
Hi
Is anyone of you aware of a marker/method to stain eosinophilic
granulocytes in mouse lung tissue which is formalin fixed and paraffin
embedded?
Thanks
Joost Bruijntjes
TNO Quality of Life
Zeist
Holland
TNO.NL http://www.tno.nl/
Joost Bruijntjes
T +31 30 694 44 80
F +31 30
Whenever I needed to prepare projection slides I coverslipped with another
regular glass slide, then cleaned up the edges after the mounting medium
dried. Low power microscopy was still possible providing the working
distance for the objective lens was greater than 1 mm.
Bryan Llewellyn
Try:
Apply this rule after message arrives with Bernie Taulin in the senders
address
Delete it
Kemlo Rogerson
e-mail kemloroger...@nhs.net if not at work.
DD 01934 647057 or extension 3311 Mob 07749 754194;
Embrace uncertainty. Hard problems rarely have easy
From: Savaloja, Lynnette C
Sent: Thursday, April 02, 2009 11:06 AM
To: Webb, Dorothy L
Cc: Semerad, Shelly A
Subject: FW: Cervical cancer mission in Peru
Importance: High
Hey Dorothy,
Got an old microtome laying around (see below)? Let me know... L ;)
Or Bernie Taupin... Even.
Kemlo Rogerson
e-mail kemloroger...@nhs.net if not at work.
DD 01934 647057 or extension 3311 Mob 07749 754194;
Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah
Lehrer
This e-mail is confidential and
I have tried for the past few days to stay out of this. Might I suggest
that if there is a person or persons causing anyone discomfort or
problems, simply add them to your blocked list in you e-mail program.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Good morning:
I have good luck by simply using haematoxylin and eosin stainingthe
eosinophils pop right out by virtue of their bright pink/red granules!
When used in conjunction with nuclear morphology, one can, with
confidence, call these cells eosinophils.
I have never performed IHC for
... such as a mouse femur, do you prefer to orient the bone parallel to the
knife edge or perpendicular to the knife edge (or diagonal)?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
I haven't checked my email for a few days! Yikes, over 150 messages! But I'll
offer this anyway, however late. Entamoeba trophs can easily be demonstrated
either in dried fixed smears or in paraffin sections by PAS. Their cytoplasm
is loaded with glycogen so they stain very dark.
Dear Histonetters,
I just wanted to say a big thank you to all who responded to my
cryoprotection request. We managed it to fix the samples with GA and
do a sucrose step accourding to your suggestions: it worked!
happy eastern to all,
Frauke
neither - I like it on the diagonal.
Andi
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724
algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097
happy slicing and
I have always orientated the specimen in an AP view and taken
longitudinal/frontal sections. As for parallel or perpendicular, I may
not be completely sure of what you mean.
Jack
On Apr 7, 2009, at 9:48 AM, Monfils, Paul pmonf...@lifespan.org
wrote:
... such as a mouse femur, do you
I for one would be lost with out this group. I need and miss the collaboration
of fellow histotechs on a daily basis. Cheri
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of alan taylor
Sent: Saturday,
Has anyone used the DAKO predilute antibody, Mammoglobin, on the Ventana
Benchmark XT? And if so.could you share the protocol you used? Finally
looking on the Dako website it appears that they have ready to use, could you
share with me which antibody should be used with the Ventana
Cynthia Keith HT(ASCP)
Histology Supervisor
NorDx
102 Campus drive
Scarborough, ME 04074
Tel: [207] 885-7907
Fax:[207] 885-7538
Email:kei...@mmc.org
CONFIDENTIALITY NOTICE: This email message, including any attachments, is for
the use of the intended recipient(s) only and may contain
Dear Histonet members:
I have removed Bernie Taupin from the list.
Let's please get back to the topic of histology and related fields.Thanks
Linda M
Histonet administrator
MKing mak...@ufl.edu 4/7/2009 8:43 AM
a motion is hereby offered to have the active members of histonet vote
this troll
We have recently been having dark nuclear staining with our AE1/AE3
antibody. This is a recent event and we cannot figure out what is going
on. It started gradually and has been increasing in intensity. We have
not changed antibody lots or any other part of our protocol. Has anyone
seen this
Good morning.
A colleague of mine recently placed the 10% formalin fixed mouse embryos in 2%
agarose, let them solidify, then process at 30 minutes per station under
pressure/vacuum. The protocol was published, but right now I don't have that
information. Hope this helps in some way!
Jen
Hi Yvan,
Does it have to be a coverslip? There are some mounting media that harden and
form a barrier with optical qualities similar to that of glass.
It might not be a perfect solution, but it might work.
I think the stuff I used was called Crystal Mount (?).
Paula :-)
Paula Sicurello
I have a HI downdraft fume extractor for $50 and we will provide carbon to
refill the drawer.
Cathy A. Mayton
Wasatch Histo Consultants, Inc.
775-625-4425
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Yes, the scintillation vials are notorious for shattering in the freezer.
However, if you put them in a plastic container with a lid on, the shattered
glass will be contained and not all over your freezer. When breaking larger
glass containers, again place them in the freezer, remove from the
I tried also this antibody on Ventana Benchmark. Pity, I had no results.
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Vickroy,
Jim
Gesendet: Dienstag, 07. April 2009 17:54
An:
When was the last time you checked the pH of your retrieval solution? Or
are you using an enzyme?
Mark Tarango
On Tue, Apr 7, 2009 at 9:53 AM, Tammy Barnhart
tammy.barnh...@mckennan.orgwrote:
We have recently been having dark nuclear staining with our AE1/AE3
antibody. This is a recent
hello, if any of you are using and/or have used the Ted Pella, Inc.
Tissue Capture Pen will you be so kind as to share with me your
experience (s), pros, cons and whatever else you deem necessary? Also,
does it's use require special glass slides? Any info you can provide on
it's use ASAP will
I can spot spam and o.t. posts and delete them in a half second each. The
questions on Histonet make me aware of the extent of use of special stains, and
the potential for new stains. Jjob postings are forwarded to the career service
office here. The safety warnings are often very useful.
We are looking for H. Pylori control tissue and also GMS/fungus control
tissue. Is there anyone out there that might have extra to share? We have
good GRAM control blocks that we would be happy to exchange. Please let me
know if you can help us out. Thank you!
Deanne Knutson
Anatomic
Dear Histoneters,
I am doing some IHC on rat cerebellar granular cells (NOT TISSUE) and
I need to fix them with PF.
I have gotten 4 different answers on how to go about this, and I
wanted to run this by the list to see what you think.
1) Fix in COLD, 4% PF in 1x PBS
2) Fix in COLD, 4% PF
Is anyone using recycled formalin for primary fixation of either surgical or
autopsy tissue? Thanks.
Richard
Richard W. Cartun, Ph.D.
Director, Histology Immunopathology
Director, Biospecimens
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860)
Sophie,
I for one would never throw a torrent of abuse at any one (mmm unless
they are politicians - in between football seasons!)
So if my (and most others on Histonet) comments are not of some worth
then we apologise.
We need to try harder.
(also please remember the delete key, I
Nancy,
Best advice I can offer is to ensure as much fixation as you can.
We start with 30 minutes at 30oC and the ramp it up to 40oC for 30-60
minutes, rinse in 70% ethanol (5min)then continue microwave processing
with isopropanol and wax. You may also consider decreasing the
processing
Possibly the concentration of hydrogen peroxide in the DAB solution has
been slowly increasing (maybe micropipette creep?)
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at
Yes. ??
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
I find that the harder I work, the
more luck I seem to have.
- Thomas Jefferson
Hi, i normally work on tissue.
I think 4% PFA in 0.1M PB is fine. so approach 3.
2009-04-08
TF
发件人: Guillermo Palchik
发送时间: 2009-04-08 05:03:13
收件人: histonet
抄送:
主题: [Histonet] Fixation question - Cerebellar granular cells
Dear Histoneters,
I am doing some IHC on rat cerebellar
Yes, Well-done to all of you who have decided to stay! It really separates the
wheat from the chaff, as it were. Or the weenies from the real adults, more or
less. And even the French-speakers from the non-French-speakers, in the case of
Kemlo/Rene. Ha ha. That was a joke. Learn to laugh a
Bernie,
I used to use the method below for FS on muscles at Emanual Hospital in
Portland OR. Since we had such wonderful success, we incorporated this method
for all FS.
Years later, I developed the MATSSE (pH3.4) 1-Step Trichrome Stain Kit
(Modified Trichrome Method for Muscle Biopsies Cut
I use isopentane suspended in liquid nitrogen, too.
sorry if this sounds curt, but due to your cut-and-pasting, im not entirely
sure what point youre trying to get at...?
From: Akemi Allison-Tacha akemiat3...@yahoo.com
To: Jennifer MacDonald
Sorry, I too noticed that the text looked weird then I did a cut and paste. I
keep all the Data Sheets I created in my files, and this was just a portion of
the Notes from the original Data sheet. I thought it might be helpful because
you stated you had difficulty with cracking.
You did not
Sorry, again, I'm confused... are you responding to me? I'm not the original
poster... I never stated I had trouble with cracking, because, well, I don't.
I'm Bernie Taupin, the King of Cryomicrotomy, Esq.
From: Akemi Allison-Tacha akemiat3...@yahoo.com
To:
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