We just received an order from Fisher in which the lab tape that was
sent was 2-inches wide, but the catalog numbers on the tapes and
description on the Web site was for 1-inch wide tape, so we did not get
what we thought we were getting either.
Esther Peters, Ph.D.
George Mason University
We can do that but what kind of volume are you looking at? Where are you
located?
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu on behalf of b427...@aol.com
Sent: Thu 7/23/2009 7:02
We do a GMS for fungus on all nails we receive. We often have a difficult time
keeping the nail tissue on the slide. We've tried baking for long periods,
pre-treating in formalin, using silane slides, with no luck. Even when the
nails cut relatively easily we still lose it, and end up
Prepare a 10% solution of Titebond II premium wood glue (found in most
hardware stores). Dip the slide in the solution just before mounting the
section on the slide. Let the slide dry and stain away.
Jim
___
James E. Staruk HT(ASCP)
www.masshistology.com
We use Albumin Solution from Sigma (A7034-10ml) on our slides. We stain
on the Artisan and or the Ventana stainer as well.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare
Thornton
Sent: Friday, July
Happy Friday Histonet Community!
The Annual NSH Symposium/Convention is fast approaching. If you haven't
signed up or checked it out yet don't hesitate too long. Workshops are
filling up we don't want you to miss out on the classes you need.
We know money is an issue for all labs all
My company can provide that service for you. Check out my website at
www.ihctech.net for a Request for Services form and list of our services and
prices.
Best regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
For really difficult tissues such as bone, cartilage and perhaps nails I
make a 5% solution of elmers glue in dih20 and dip regular uncoated slides
in the glue, let them air dry. Use the glue coated slides to pick up
sections, just dipping in the water bath seems to activate the glue enough
to
Hi Histonetters!
I hope everybody is doing well and finding a way to stay cool on this
hot summer day!
RELIA Solutions, The nations only recruiting firm dedicated to the
permanent placement of histology professionals is currently working with
2 premier clients in the Charlotte, NC area who are
Does anyone have experience with this stain on ffpe rat tissue?
Best regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
email pru...@ihctech.net
website www.ihctech.net
Just wondering why you use Titebond II? We use plain old Elmer's Glue.
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
No trees were hurt in the sending of this email
We had a series of PIN 4 cocktail immuno stains and got a poor reaction.
I am afraid to cut deeper in the block and miss the area of concern.
I have determined the problem was due to the heat bar not being turned
on.
What can I do the slides to reverse the process and restain them. The
DAB
We have had similar problems with the Vulcan Fast Red not staining. It was
suggested by the vendor to buy the fast red in smaller amounts (they actually
repackaged the product) so that the chromogen was not being taken out of the
fridge and put back in too many times. Depending on your
We soak our nails in 20% sodium hydroxide for at least an hour, rinse in
running tap water for a few minutes before we place the cassettes on the
tissue processor. We use positive charged slides, heat slides in 80 degree
oven for 20 minutes and perform a PAS/fungus on the slides. The PAS is a
Immerse it in water fully inflated and see how much water is displaced;
is that Avogadro hypothesis or some other Greek dude?
Kemlo Rogerson
e-mail kemloroger...@nhs.net if not at work.
DD 01934 647057 or extension 3311 Mob 07749 754194;
Embrace uncertainty.
Dear Members,
I am trying to standardize the oil red o staining method in murine frozen
tissues using Churukian method.
I am having following queries.
1. The type and source of aqueous dextrin used in the staining procedure.
Search in sigma catalogue provided different types of dextrin (dextrin
I agree. We also have the problem with timers in our lab. Someone's goes
off and we try to figure out whose it is. Couldn't they make ringtones for
timers? :-)
--On Thursday, July 23, 2009 8:54 PM -0700 connie grubaugh
conniegruba...@hotmail.com wrote:
The other day just about every
Good Morning Histoland, FYI. We use fisher ink-jet plus slides with clipped
corners. Recently we received a case labeled correctly that were just plain
slides. We did not notice it till we had used a few of the boxes of slides.
It was not to big a disaster for our lab, but it might be for
I always thought it was odd that our $36000 processor (which costs more
than our first HOUSE) made a grinding blap noise when it was done!
You'd think for that amount of money you could get a pleasant (sexy)
voice that says, Good day! I have finished making your life easier.
Please come and attend
My immuno stainer talks to me in a sexy voice!
But now the issue is should you be able to request if you want a female or male
voice?
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email:
For the prices we pay now they should offer to clean the lab!!
Pam Marcum
- Original Message -
From: Sara Breeden sbree...@nmda.nmsu.edu
To: histonet@lists.utsouthwestern.edu
Sent: Friday, July 24, 2009 9:35:17 AM GMT -05:00 US/Canada Eastern
Subject: [Histonet] Bells
To all:
I apologize for asking for this information--I deleted the email communication
re: special coverslips to be used on automatic coverslippers! We have recently
been approved to order a slide stainer and coverslipper and several people
commented on specific types of coverglass to use.
I
Funny you should bring up this subject, Connie. Two of us work night shift in
our Pathology department, so it should be nice and quiet!
All we are supposed to do is embed and cut and stain the H and Es. Do you know
how hard it is to ignore immuno and special stainers and PTS alarms and
ThinPrep
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