We have Ventana IHC instruments and Ventana Vantage for labeling, tracking, QA,
workload stats, etc, etc. It allows for the best process flow you can
imagine. I'm very into processes and this system will reduce if not
eliminate your errors. We were already a LEAN lab and with the addition
We don't. We buy a 5-gallon cubitainer which sits on a counter. First, it
would be inconvenient to the users to have to constantly go into the flammable
cabinet to access. Second, it comes in a cardboard container which is not
allowed in a flammable cabinet (per Fire Department).
We have an
No, it is ridiculous. Safety people tried to argue this with us years ago.
One of our pathologists told them, How can something that is almost 90%
water be a combustion hazard?
- Original Message -
From: Riesen, Rebecca rebecca.rie...@nchmd.org
To: histonet@lists.utsouthwestern.edu
I was just wondering how others are tracking mistakes. We have a form that the
employees fill out when they catch and rersolve mistakes. I feel this gives me
the opportunity to recognize problem areas (or people). I was approached by
one of my staff who feels that this may take away from a
Janice, may I ask whom you have for your LIS?
Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369
-Original Message-
From: Mahoney,Janice A [mailto:janice.maho...@alegent.org]
Sent: Friday, September 11, 2009 9:14 AM
To: 'Michael Mihalik';
I once had my safety officer insist I wear chain maille gloves while
cutting frozen sections. They didn' t care about all the reasons I gave
them why I shouldn't - like it would be impossible to use the machine
while wearing them, and the patient would have to lie on the operating
table
I have to ask--what was the point of chain mail gloves?!
Emily
One of the defining characteristics of modern surgery was that patients
ought to survive it.
--Peter Stanley, For Fear of Pain: British Surgery, 1790-1850
On Fri, Sep 11, 2009 at 9:31 AM, Jackie M O'Connor
No. We store only the 37% in a flammable cabinet. Ten percent formalin
is not considered a flammable substance according to the MSDS for Richard
Allen 10% formalin. Which brand are you using. Your storage should be
according to the manufacturer's MSDS. Grant it, they should be all on
the
To prevent getting cut on the knife.
Emily Sours talulahg...@gmail.com
09/11/2009 08:34 AM
To
Jackie M O'Connor Jackie.O'con...@abbott.com,
histonet@lists.utsouthwestern.edu
cc
Subject
Re: [Histonet] formalin storage
I have to ask--what was the point of chain mail gloves?!
Emily
We store all our chemicals in a flammable storage cabinet
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Riesen,
Rebecca
Sent: Friday, September 11, 2009 9:16 AM
To: histonet@lists.utsouthwestern.edu
Does he require his wife to wear chain mail gloves while paring potatoes?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie M
O'Connor
Sent: Friday, September 11, 2009 9:31 AM
To: Jean Warren
Cc:
Janice, I have a few responses here, but let me give you one of them, for
now.
An interface cost roughly 10,000 per 'side'... so 20,000 without
negotiation. What does Vantage cost?
I'm not looking for your specific pricing, just ballpark pricing.
Michael Mihalik
PathView Systems | cell:
Cerner Millennium
-Original Message-
From: Michael Mihalik [mailto:m...@pathview.com]
Sent: Friday, September 11, 2009 8:31 AM
To: Mahoney,Janice A; 'Cordova, Jean'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide labels used with IHC Instruments
Janice, may I ask whom
Amy:
Exactly what do you mean by 'we store all our chemicals in a flammable
storage cabinet'? By any chance, do you mean that you actually put
everything into the same cabinet together? I would be very concerned
about putting everything into one cabinet (flammable or not). Some
materials
We do it this way also. The person catching the error fills out the form (even
if they caused the error!) and gives it to our supervisor. Then its up to his
discretion what to do with the information.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600
I have also had 'general' safety inspectors (OSHA people who mostly
focus on meat packing plants and food processors) make the same claim
that we had to use metal or other cut resistant gloves. I had to
demonstrate the task of cutting a frozen section to get them to
understand. They still did
All,
I am looking for an automated immunostainer for research purposes. I
would like something reliable, easy to use, and compatible with a wide
variety of reagents and protocols. Does anyone have any recommendations?
We are considering upgrading our lab from a manual lab to fully
automated.
Thank you all! I have received many responses already concerning 10%
Neutral Buffered Formalin (NBF) storage. Only one person has stated
that they store all chemicals, including formalin, in safety cabinets.
I just want to clarify something. All MSDS's I studied stated that 10%
NBF indeed is
never store everything in one cabinet.
As far as i know, HCl and formalin should not be stored together. If any of
you know why...
-- Original --
From: Riesen, Rebeccarebecca.rie...@nchmd.org;
Date: Fri, Sep 11, 2009 10:09 PM
To:
For veterinary diagnostic lab personnel:
We're having a difference of opinion here over how long our Histo slides need
to be retained. It was my understanding that all paraffin blocks and stained
slides need to be retained for 7 years. Others here believe that as long as
the blocks are
Just so a stereotype doesn't get started here...I was 1st taught in the
(straight) use of Permount by such an Asian technician (who couldn't speak
English, but was such a wonderful teacher I learned more from watching her
than anything!) :-)
--On Thursday, September 10, 2009 1:52 PM -0700
We too were told by our internal safety compliance officer that 10% NBF has to
be stored in flammable cabinets and that is now how we store it. We were also
told that our general purpose household bleach has to be in a flammable
cabinet
Erin Martin
UCSF Dermatopathology
that makes sense now...when we make the stuff, we ALWAYS do it in a fume
hood...
--On Friday, September 11, 2009 10:09 AM -0400 Riesen, Rebecca
rebecca.rie...@nchmd.org wrote:
Thank you all! I have received many responses already concerning 10%
Neutral Buffered Formalin (NBF) storage.
Jan, we'll be at APIII in 10 days so it's going to be hard to turn around
and be at NSH, but we're trying. I'd be very happy to get your and other
people's opinion of our system. We think we have a very straight forward
and comprehensive approach to workflow processing. It's really an unfair,
Excellent stainer, but you need to look at your process to help facilitate the
technology. if you have nay questions you can reach me.
Jesus Ellin
Yuma Regional Medical Center
928-336-1144
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Gomez, Milton
I always diluted Permount to give it the fluidity I desired for manual
coverslipping and faster drying.
René J.
--- On Thu, 9/10/09, Leslie Chen lc...@mednet.ucla.edu wrote:
From: Leslie Chen lc...@mednet.ucla.edu
Subject: [Histonet] Permount question
To: histonet@lists.utsouthwestern.edu
Hi Michael,
I have been reading with great curiosity your postings on Histonet. Other
vendors have been shot in the knees for doing what you appear to be doing!
You are openly soliciting!
I personally don't care to read your opinions about the various LIS and LEAN
based systems that are
I always used the DAKO autostainer but lately there have been some costs issues
raised in HistoNet as well as many very good opinions about the BondMax from
Leica. You could check them both or ask for a demo.
René J.
--- On Fri, 9/11/09, Kalleberg, Kristopher kristopher.kalleb...@unilever.com
Akemi,
We follow pretty much what you outline below. I believe following strict and
comprehensive validation will save you headaches later on. You want to 1)
convince yourself that the antibody works as advertised and as expected
according to the literature and 2)Identify and solve any
Other vendors have been shot in the knees for doing what you appear
to be doing! You are openly soliciting!
Since we're on the subject, I also wish vendors would stop searching the
Histonet Archives for certain keywords involving their products, then
cold-calling/emailing/spamming people who
Dear Histonetters,
I quite need answer as soon as it possible, PLEASE!
I am working with mouse tissue and tumors. What type of paraffin is the best
for processing and embedding to cut 4µ nice sections?
Thanks in advance,
Naira
___
Histonet mailing
Paraplast
René J.
--- On Fri, 9/11/09, Margaryan, Naira nmargar...@childrensmemorial.org wrote:
From: Margaryan, Naira nmargar...@childrensmemorial.org
Subject: [Histonet] type of paraffin and polymer
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Friday,
Paraffin Prills from Polyscientific RD is great for animal and human work.
Pam Marcum
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan,
Naira
Sent: Friday, September 11, 2009 12:10 PM
To:
Sheila, I apologize to you and Peter and to whomever else I have offended.
I view Histonet as a place where people can go for knowledge and where
people can share their experiences.
I was trying to share my experience with LIS systems and information system
technology in general. I do admit
yes, I fully admit that there was some component of publicity.
However, I do feel like it was 90/10 or 80/20 knowledge vs publicity.
I guess that's the problem. As a non-commercial list, the ratio should
be more like 100/0.
I don't know if you're from Ventana or Daka or whomever
I'm from
Full disclosure: My company manufactures an LED fluorescence microscope :-)
Merced's information from the Zeiss rep is partially correct and needs some
clarification. There is a gap in the spectrum of available LED's from 530 to
590 nm that would be powerful enough for fluorescence excitation.
--
We work out the specifics of the protocol for each new antibody with variables
such as dilution, heat or enzyme retrieval, detection system and antibody
incubation times based on the manufacturer's recommendations in the insert for
the antibody. We do this
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Hello Histonetters,
I am looking for info on any company that carries the Weck handles. I have
blades that will work on this handle but the company that we get these from
does not have the handles.
Thanks for any help.
Sharon Campbell, HTL(ASCP)CM, BSBM
Histology Supervisor
Celligent
Hi everybody,
I have been doing Sirius Red for a while with good luck. Today we made fresh
solutions and after the stain was done, the sections (rat liver) are too red.
They are positive, the fibrosis is bright pink, but the background is pink as
well. I got a slide back to water and distained
Formaldehyde is flammable; formalin is not. Above 122 degrees Fahrenheit
enough formaldehyde evaporates from formalin to create a modest fire hazard in
the fumes just above the liquid. Try this: pour 3 ml of formalin (37%
formaldehyde) into a watch glass under a fume hood (fan off). Touch a
Additionally, we have been told that the reaction product is also a very
potent carcinogen and that is the reason why you are supposed to
thoroughly rinse tissue free of unbound formalin prior to immersion in
HCl based decalcification agents and once again when removing from decal
and placing the
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