Collagen cannot be expected to stain uniformly throughout, since "collagen"
is a blanket term for at least 10 different chemical substances; therefore,
certain areas of the collagen will take on the dye with different intensity.
I like the comments made by Geoff about fixation and removing the par
Thank You Fellow Histonetters, I greatly appreciate all the advice.
Have a great week end.
Stella
On Fri, Oct 23, 2009 at 12:52 PM, Thomas Jasper wrote:
> One more time.
> tj
>
> -Original Message-
> From: Thomas Jasper
> Sent: Friday, October 23, 2009 10:41 AM
> To: 'Stella Mireles'
>
I just read of the plant drama for the lab getting a CAP Phase 1 ding. There
was a study done ages and ages ago and certain plants IMPROVE the air quality
in chemically contaminated environments. (If you have any measure of our
solvents in the air-your air is contaminated even if it is an allow
Hi,
Does anyone have any suggestions as to why our mouse glomeruli are
shrinking after coverlipping in DPX? Immunohistochemistry is performed on PLP
frozen cryostat sections, and the glomeruli and tubules look fine
upon checking after the chromagen is developed, but once they are
dehydrated, clea
Dish washer dude :-D
That's a really awesome story, almost too much clichée to be true.
Best Friday spam topic anyway.
Bader Siddiki schrieb:
Everybody is writing about the plants in the histology lab.
May be I will share my experience with you in Biochemistry lab at MSU .
Many many years ago
Hi everybody
We have had the Sakura disposable blade system for many years. I do recall
that we had several rotary and sledge blades that I took to the local scrap
metal company, had them weighed and cashed them in, putting the resulting
several pounds (£'s)in the Christmas box to go towards t
No way, I still use mine, especially the tungsten ones, but I still do some
plastic embedding of bone, another place I use mine is in the cryostat to
make tape sections of calcified bone.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-85
OMG... now that is the best story I have heard all day!
Thank you for making me laugh :)
Maria
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bader Siddiki
Sent: Friday, October 23, 2009 1:44 PM
To: Histo
Everybody is writing about the plants in the histology lab.
May be I will share my experience with you in Biochemistry lab at MSU .
Many many years ago when I was a post doc in Biochemistry dept. I had lots
of plants in the window.
One day when I came from lunch, I saw unusual plants along with min
I don't know about plants in the lab (never had any) but I recall a problem
from years ago. It was a hot summer day and the air conditioning was having
problems, so we opened a couple of screened windows in the lab. At the end of
the day one of the techs who was reviewing the slides that had j
I just threw one out the other day - put it in a sharps container.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce
Cline
Sent: Thursday, October 22, 2009 9:52 AM
To: histonet@lists.utsouthwestern.edu
S
Sheila:
1- For tissue processing use isopropanol mixed with mineral oil.
2- For dewaxing the sections before staining use Dish Washing soap.
3- To prepare the slides before coverslipping don't use anything, just oven dry
the sections and cover.
Under separate cover I am sending the detailed proced
Liz,
Great point, and definitely something to keep in mind should one do that
stain. Indeed, CD14 is not specific to macrophages only (monocytes also
express the antigen as discussed).
Of course, there are other more specific macrophage markers (i.e. HAM56)
but these are commonly developed in mi
> Please unsubscribe... thankyou
I'd rather not... I quite like this list! But thanks for the advice...?
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Tim Morken
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Thursday, October 22, 2009 12:15 PM
To: 'Breeden, Sara'; histonet@lis
How has everyone disposed of their old "permanent" microtome knives?
Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
* CONFIDENTIALITY NOTICE * This message contains confidential
information and is intended o
Hi all,
Our Joint Commission audit was just completed (first time for JC for me). We
passed almost everything fine.
The one thing they came up with is that we don't use "negative controls" for
most of the special stains - like trichrome, congo red, etc. We use negative
controls for micro-organ
Test
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Our Joint commission audit was just completed (first time for JC for me). We
passed almost everything fine.
The one thing they came up with is that we don't use "negative controls" for
most of the special stains - like trichrome, congo red, etc. We use negative
controls for micro-organism cases
Hi all. Could someone recommend a xylene substitute and mounting media to use
for staining and coverslipping (no vendors please)? I am considering processing
without xylene and would like to discontinue everywhere if possible. My
previous experience was a lifetime ago and I'm sure things have ad
Please unsubscribe... thankyou
Confidentiality Notice: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
confidential and privileged information. Any unauthorized review, use,
disclosure or distribution is prohibited. If you are not
She is asking to stain for macrophages and that's a monocyte/macrophage
marker so that's not the same. You have to be careful with the names of
antibodies and check the specification sheets. You can search for a
macrophage marker and the antibody may be listed as a macrophage marker
as the follow
Hi Naira,
Our new IVD rabbit monoclonal (anti human) CD14 is preferentially
expressed on monocytes/macrophages:
http://www.cellmarque.com/07/p_detail.php?id=2066
Best regards,
Isaac Milos
Cell Marque Corp.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histo
Good morning Histonet.
Thanks a lot to so many people who helped me with my Leica XL question - your
wealth of knowledge never ceases to impress me.
I have a different question now about Masson's tri-chrome and it's specificity
on isolated rabbit tendon. There is some muscle still attached to
One more time.
tj
-Original Message-
From: Thomas Jasper
Sent: Friday, October 23, 2009 10:41 AM
To: 'Stella Mireles'
Subject: RE: [Histonet] Floaters in Waterbath
Others may mention this to you...and it can get a little political. Do
not discount the grossing bench. Whether it's the
Hello,
I was wondering what your guys protocol on working in the histology lab and
gross room with formalin and xylene in use (without charcoal filtration), is
when the ventilation (exhaust) system goes down. Do you have respirators to
wear or do you evacuate everyone until the ventilation sys
Tannic acid in the fix, both in the glut and the osmium if I remember
correctly, will help but I don't think it is absolutely necessary.
Check out any of the big cardiology texts, there will be a chapter or
two on normal cardiac anatomy from the gross level down to EM level.
Look at the EMs and
The first things to check are 1. adequate fixation. 2. Complete removal
of paraffin.
That done, I don't think you can use staining intensity to draw any
conclusions about the health, or lack thereof, of collagen.
I suggest finding some references on collagen production and degradation
before pro
Hi all.
I need to study gap junctions in heart tissue by electron microscopy. Does
anyone know if I need some specific staining to see the gap junctions?
Thanks a lot.
Mercè Martí Gaudes
Abans d'imprimir aquest missatge, si us plau, comprova que és realment
necessari. El
Naira -
These are presumably SCID mice? You can use a mouse mAb with no Ig
interference, since SCIDs do not have an immune system. You can get the
rare 'leaker', but I've used murine mAb routinely for human xenografts in
SCIDs. Now, Nudes are a different story.
From:
"Margaryan, Naira"
I have an investigator looking at Massons(with Aniline Blue), Van Gieson
and Gomori's all showing the same type of collagen staining. In some
areas collagen fibers are brilliantly stained and other fibers are pale
all on the same slide. This is consistent across the board so we know
it is not the
Liz:
Thanks for clarifying. Your comments are spot on. That's what I get
for responding too quickly and not thinking things through.
I would agree with Naira that red detection is useful in the melanoma
context.
Joe
380 MRC
4-4737 (voice)
3-3482 (fax)
pager 131-1170
joseph-galbra...@uiowa.ed
That will work but I'm not aware of the non-mouse anti-human macrophage
marker, you could check the web or biocompare.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Concerning Control Tissue Blocks:
The NSH provides and Share and Exchange Service for members and non-members
alike. Anytime you have a need for a particular type of control, please contact
the NSH office (nsh.org) and we will try and meet your needs. The program is
only intended for individua
Thanks a lot both of you!
For melanoma detection, I usually use HRP with AEC. Is there any non-mouse
anti-human macrophage marker?
Thank you much,
Naira
-Original Message-
From: Liz Chlipala [mailto:l...@premierlab.com]
Sent: Friday, October 23, 2009 10:51 AM
To: Galbraith, Joe; Marg
F4/80 is a mouse macrophage marker. If she wants to detect human
macrophages in a mouse background she will need to use a mouse
anti-human CD68. She will need to run it with a mouse on mouse
detection system and run all of the appropriate negative controls. I
would also select a alkaline phospha
Naira:
Here is a link to a site listing macrophage markers. F4/80 is a
commonly mentioned marker for macrophages. I presume you mean IHC
rather than ICH. Enjoy.
http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm
Joe
joseph-galbra...@uiowa.edu
-Original Message-
Hi Histonetters,
I would like to be able to look at human macrophages in human melanoma
xenografts raised in mouse. Could you please suggest me a best Ab for ICH and
protocol?
Thanks in advance,
Naira
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Don't go to the web site. It has a picture of the water and boats! It will
just drive you batty!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dana Settembre
Sent: Friday, October 23, 2009 11:11 AM
T
Seattle, WA
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential o
Where is PhenoPath?
Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJUSA
>>> Merced M Leiker 10/23/09 9:59 AM >>>
Yes that's exactly what I was going to say - I'd like to toss in my
resume,
please! :-)
--On Thursday, October 22, 2009 10:02 PM -0500 I
I will be out of the office starting 10/22/2009 and will not return until
10/26/2009.
IF YOU NEED IMMEDIATE ASSISANCE PLEASE CONTACT ANDREAS KAEPPLEIN.
__
This email has been scanned by the MessageLabs Email Security System.
Kim wipes work great, and if done after each block shouldn't slow things down
much. Besides, what's the point of quantity when the quality is compromised
with floaters. You should have a policy regarding this since it is a CAP
requirement.
Ruth Cazares, HT (ASCP)
Histology Supervisor
Departmen
Hi Stella, Not only wiping the top of the waterbath water with kimwipes between
each block, and keeping forceps clean at embedding, and keeping your slides
clean, but also keeping things clean at grossing: clean cutting board and
instruments between tissues or cases. One pathologist called it fo
We currently have a Quality Improvement Plan in effect to address this issue.
Jackie is right about keeping those forcep wells clean.
Although we don't swipe Kimwipes over our waterbath after each block, we do it
very regularly.
Another thing to consider is how often you clean your embedding mold
Hair net and gloves??
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles
Sent: 23 October 2009 15:11
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Floaters in Waterbath
I know we ha
Kim wipes seem to pick up more debris than paper towels, and they pick up
much less water. We routinely sweep the waterbath with a kimwipe after
each block. You can also pick up floaters from embedding if the forceps
are not cleaned between each block. Most embedding centers have multiple
Kim Wipes pulled across the top of the water will pick up most, if not all
floaters. Very thin so they don't deplete the water bath. Should be done
after each block to prevent floaters.
Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical
Center I
200 Medical Park Boulev
I know we have all had some problems with floaters in our waterbath at some
point in our microtomy career.
Our doctors are very picky and I need some tips on keeping an immaculate
clean waterbath, but not sacrificing the speed in a regular
routine lab. We use the pyrex waterbath and paper towels f
Yes that's exactly what I was going to say - I'd like to toss in my resume,
please! :-)
--On Thursday, October 22, 2009 10:02 PM -0500 Ingles Claire
wrote:
# 1- Any openings?
2#- I think the ships would depress me a bit as I would constantly be
reminded that I am not ON said luxury ship. 3#
In response to the Tau antibody I have an antibody and protocol that works well
if you are interested, but the antibody is not from Biocare.
Message: 10
Date: Thu, 22 Oct 2009 14:56:53 -0400
From: "Angela Bitting"
Subject: [Histonet] anti-Tau on BenchmarkXT
To: "histonet"
Message-ID: <4ae072b6
What a great guy Dr. Gown is!! Kudos, Dr. Gown. I'm printing this email
and leaving it in strategic places in the lab!
Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical
Center I
200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F:
804-765-5582 I
People have much the same effect.
Lesley Weston.
On 22-Oct-09, at 12:35 PM, Haynes, MaryAnne wrote:
Some Health departments state that plants and their potting soil
can be a potential microbial and fungi contaminate in the lab.
Mary Anne Haynes
Mary Anne D. Haynes, MBHA, DLM, SLS(ASCP)
Pat
Not only will I not have a window in my new lab, but there's no chance
of piping in sunlight to where I'll be! However, I have moved up one
floor and will no longer be in the basement and my new lab is gorgeous
and roomy and has actual air quality capability! A step up for
histology person-kind!
This had nothing to do with an inspection, but follows this comment. There were
3 plants in our lab and there was one histotech who starting sneezing and got
congested whenever he sat at his microtome. The plants were in front of him on
a raised shelf. Finally we had a thought it may be the plan
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