RE: [Histonet] Preventing slide labeling mistakes

You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM

Re: [Histonet] Preventing slide labeling mistakes

Lynette, You might want to also investigate different fonts if that is an option. Keeping the same size but using a different font can make a world of difference. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE

RE: [Histonet] Preventing slide labeling mistakes

Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich;

[Histonet] CAP guidelines for positive controls

Hello, I am trying to find the guidelines for CAP that describes how long IHC positive control slides are to be kept on file ... does anyone know? Thanks in advance! Tiana This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally

[Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports

What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks

[Histonet] course recommendation

I am looking for a conference/course to attend in 2010. I am experienced in IF and IHC and would like to learn more about any new techniques and products that might be available. Does anyone have a recommendation for a course or a conference in the US? I am not a certified HT but I pretend to

Re: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports

We used to add that the methods were experimental. René J. --- On Fri, 11/20/09, Foshey, Annette afos...@chw.org wrote: From: Foshey, Annette afos...@chw.org Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports To: (histonet@lists.utsouthwestern.edu)

AW: [Histonet] Preventing slide labeling mistakes

We write a macro-protocol at the time of grossing with all blocknumbers and additional identifer. This paper is matched the first time with all blocks after embedding and before cutting. Missing blocks or wrong labelled blocks are found. The second time the paper is matched with all slides after

RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathologyreports

We add ours to only the Class I ASR cases. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] CAP guidelines for positive controls

They should be kept to comply with your policies and the state requirements. Remember that CAP has regulations but cannot override state nor institutional policies. René J. --- On Fri, 11/20/09, Tiana Fountain tfount...@exchange.hsc.mb.ca wrote: From: Tiana Fountain

RE: [Histonet] course recommendation

Take a look at the National Society for Histotechnology (www.nsh.org). There are several State and Regional Society meetings coming up in the spring of 2010 and then of course there is the National Symposium/Convention in the Fall of 2010 in Seattle, WA. Jack Ratliff NSH -Hard Tissue

RE: [Histonet] Preventing slide labeling mistakes

Linda, Yes, I should have said matching blocks and slides after staining catches mistakes but does not eliminate them. We are looking at: isolating block to be cut, labeling slide just before or after sectioning, matching block to slide after sectioning at the cutting station. Unfortunately

[Histonet] frozen sections not adhering to slide

I am processing adult rat brain sections for routine histological stains, but am having a terrible time getting them to stick to the slide. After transcardial perfusion the rat brains are dissected out and processed for frozen sectioning. I post-fix overnight in PFA, cryoprotect in

RE: SPAM-LOW: [Histonet] course recommendation

Check out this Applied Immunohistochemistry and Molecular Pathology (AIMP) meeting coming at end of January 2009 in Florida. the fourth annual international retreat on applied IHC and molecular pathology. Please visit http://www.pathlearning.com/Pathology_Learning_Centers/Welcome.html for

RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathologyreports

We use the statement for all reports that any IHC/ISH, regardless if they are ASRs or not. Martha Ward Wake Forest University Baptist Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Foshey,

[Histonet] frozen sections not adhering to slide

I am processing adult rat brain sections for routine histological stains, but am having a terrible time getting them to stick to the slide. After transcardial perfusion the rat brains are dissected out and processed for frozen sectioning. I post-fix overnight in PFA, cryoprotect in

RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports

The disclaimer is only for ASR antibodies. They don't have to be labeled experimental because a CLIA certified lab has full capability and authority to validate any antibody they want to use for any purpose. You do have to document your validation procedure and results. You can also use RUO

RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer onpathology reports

Just can't bill for RUO, correct? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, November 20, 2009 13:24 To: Foshey, Annette; (histonet@lists.utsouthwestern.edu) Subject: RE:

[Histonet] NYState licensing examination

Dear Histonetters, I am collecting any hand-outs, pamphlets, archives, books, CD's, anything you may have extra or may be willing to share that can help me prepare for the New York State Histologic Technician Examination. The examination may be similar to the HT and HTL exam; so anything

[Histonet] ISH

Dear Histonetters, I have a problem with my ISH and maybe one of you can help me. I used probes I *know* they are working. I put the mice embryos (E 10.5) in BM Purple for staining. Unfortunately they turned black in a short time. They were not stained in the inside. It looks like that the

[Histonet] Lumpectomy question

Good Friday Afternoon, Does anyone know if there is a modifier for 88307 for Lumpectomies when 20-30 blocks are submitted. We are currently charging 88307 but the pathologist thinks there should be a modifier. Thanks. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical

[Histonet] CMS/NCCI Update Dated October 1, 2009

According the one of our pathologist the above mentioned update clarified that CMS allows the billing of special stains performed on different blocks of the same Pathology specimen. Previously, it was understood that a special stain could only be billed once per specimen. Now it appears that a

Re: [Histonet] CMS/NCCI Update Dated October 1, 2009

Joyce, That's very interesting, as I remember going to great lengths a few years ago to be sure we had a billing limiter in place in CoPath to prevent the multiple charging of CPT codes on the same specimen. However, I looked up the document released on 10/1/09 and here's what I found in regards

[Histonet] anti-human Notch4

Hi friends, My PI is asking for Notch 4 IHC on FFPE tissue. Can any of you suggest me a good Ab (preferably mot mouse but anti-human Notch4) and protocol please! Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300

RE: [Histonet] anti-human Notch4

I am interested in the same antibody. Thanks, Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -Original Message- From:

Re: [Histonet] Lumpectomy question

Debbie, as far as I know, there is no modifier, all you can charge is a 88307. That's the same case when you have a skin excision at a 88305. Even if it's a melanoma excision and it takes 20 blocks, you can only charge an 88305. The system is FAR from perfect. JTT - Original Message

Re: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer onpathology reports

I have a question similar to this one. If I take 2 IVD antibodies say cytokeratin AE1/AE3 and 34BF12 and make these into one cytokeratin cocktail, is this considered an ASR because I combined them into another antibody? Joe - Original Message - From: Morken, Tim

Re: [Histonet] rhodanin stain fading

Alcohol will cause the stain to fade, even the small amt. that is carried over to the xylene over the course of  the day. Make sure your xylene is fresh and coverslip immediately after staining Rena Fail - Original Message From: Gudrun Lang gu.l...@gmx.at To:

AW: [Histonet] rhodanin stain fading

Rena, we use butylacetat for clearing and before coverslipping with Pertex (xylolbased, resinous). Butylacetat tolerates ethanol-carryover more than xylene, but evapourates very fast after couverslipping. But is it possible, that butylacetat or Pertex itself cause the fading? Do you think