Now that we can no longer use mercury-containing fixatives, I don't
think that the various proprietary fixatives advocated for bone marrow
add anything to the morphology, and some of them can gum up processors
or interfere with immunohistochemistry.
Neutral buffered formalin requires time for
My brief experience with paraffin and fluorescence (DAPI and EGFP) is that
the paraffin embedding process destroys it.
Regards,
Merced
--On Tuesday, March 02, 2010 3:25 PM -0600 Fabrice gankam
gan...@googlemail.com wrote:
Hi,
Just wondering if anyone has use hydroethinide to detect the
Acetic Zinc Formalin
Disclaimer: The information in this message is confidential. If you are not
the intended recipient, do not disclose, copy, or distribute this message, and
please immediately contact the sender.
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Amy,
We use the B+ fixative available from BBC Biochemical (
http://www.bbcus.com/products.html?pc=19pid=57 )
My pathologists love it!
Michelle
Message: 3
Date: Tue, 02 Mar 2010 14:12:04 -0500
From: Amy Farnan farn...@nehealth.com
Subject: [Histonet] bone marrow fixative
To:
We are using the Leica Autostainer XL, bridge and coverslipper (CV5030). I
think they're great!
Michelle
_
Hotmail: Trusted email with Microsoft’s powerful SPAM protection.
Rat anti-mouse F480 from serotec works hwell. It is crucial that you use a
secondary against rat IgG that is highly cross adsorbed to many species,
including mouse. I suggest whole IgG of donkey anti-rat IgG from Jackson
Immunoresearch. Just make sure to pick the version adsorbed against mouse.
Following adequate fixation of predominantly fatty breast tissue, what factors
could lead to significant shrinkage of adipose tissue around a fibrous lesion
(by ~ 50-80%) ?
_
IM on the
Rat anti-mouse F480 from serotec works hwell. It is crucial that you use a
secondary against rat IgG that is highly cross adsorbed to many species,
including mouse. I suggest whole IgG of donkey anti-rat IgG from Jackson
Immunoresearch. Just make sure to pick the version adsorbed against mouse.
You have assumed that processing is correct, but shrinkage is always caused by
too fast dehydration, therefore the processing is not adequate.
René J.
--- On Wed, 3/3/10, Dinesh Sariya sari...@hotmail.com wrote:
From: Dinesh Sariya sari...@hotmail.com
Subject: [Histonet] Processing of fatty
Sorry to post this again but had problems posting messages lately.
Here is my problem, We do not have cryostat in our facility and I will like
to test free radical production in situ in rat brain tissue
Some of the methods involves homogenate of whole part of brain and assay
with component
I second the Prisma with attached coverslipper. We have been using it now
for the past 2 months or so.
It's really a non-hassle, state of the art piece of equipment.
Paula
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Ultram Fixative is actually Ultrum Fixative and is available thru
American Mastertech:
http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStylesitem=FXULTGAL
Cindy Dubois
Message: 20
Date: Tue, 2 Mar 2010 20:08:23 -0500
From: Robert Richmond rsrichm...@gmail.com
Subject: [Histonet]
we have a ventana benchmark and use rabbit and mouse negative controls, is
there anyone out there that uses a universal control and is that ok with your
cap inspection? thanks so much
antia dudley
providence hosp
mobile alabama
Anita,
If you're referring to the reagents you use, we use a Universal Negative
Control Serum (Biocare) on our negative control slides. We have 3
Ventana instruments and have never been cited.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland
I am glad you decided on that one Paula. It is wonderful
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
From: Paula Lucas plu...@biopath.org
To: Liz Chlipala l...@premierlab.com; histonet@lists.utsouthwestern.edu;
VickiGauch
Hi, I am from Argentina and I have to perform an immunehistochemstry
with an antigen recovery using AutoZyme from Biomedia Foster City, CA. I
looked for in google and I found a lot papers citations but I could find
the company, Biomedia, to ask for a quotation. Has anybody got a contact
from this
Hi,
The Colorado Society of Histotechnology (CSH) meeting will be held at the YMCA
or Rockies in Estes Park, CO, April 23rd 24th, 2010. If you are interested in
attending the meeting and have not registered, please visit our website at
http://www.coloradohisto.org/2010/meeting.htm. Online
Hi Andrea,
Jackson lists a couple of FITC-conjugated donkey anti-rat secondaries:
712-095-150http://www.jacksonimmuno.com/MERCHANT2/merchant.mv?Screen=PRODProduct_Code=712-095-150
-
Whole Donkey Anti-Rat IgG (H+L).
According to the MSDS sheet at the web address provided by Cindy Dubois, this
solution contains water, sodium acetate, zinc chloride, phenol and citric acid
- concentrations not disclosed; pH not stated. Another MSDS for ultrum at
http://siri.org/msds/f2/bzr/bzrjb.html lists the ingredients as
Hi all
I was wondering if anyone can send me please the optimal paraffin
processing times for brain autopsy tissue.
Currently the blocks have cut and stained well but 4-5mm thick tissue
has expanded (bulged out) following cutting as if they had absorbed water.
Solutions been all
we used to do one hour in each step formalin through to paraffin (2 steps of
paraffin).
But 4 to 5mm is a little on the thick side. We used to try for 3 mm. But
didn't always get it that way.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of
Our hospital uses Meditech and in the near future we are going to go to CPOE
(computerized physician order entry). We currently are the only dept that uses
paper requisitions for Surgical Specimens and Cytology. For those of you out
there that has gone through this transition, can you share
Has anyone tried using butyl alcohol as a dehydrating and clearing agent
on there tissues? What are some pros and cons people have experienced
with using Butyl alcohol?
Todd Krueger
HTL(ASCP)CM
Boston Scientific
2 Scimed Place, P121
Osseo, MN 55311
Phone: 763-694-5709
Fax: 763-694-5505
Richard Cartun asked about Ultram fixative, which Cindy Dubois
informs us is actually Ultrum fixative.
According to its MSDS (dated 2003), Ultrum II tissue fixative contains
glutaraldehyde, phenol, zinc chloride, sodium acetate, citric acid,
and water.
It is offered by American Master*Tech
Hi Netters!!
Does anyone have an extra basket that I can borrow or purchase for the K1000
VIP? Doctor wants to start processing but basket wasn't included in processor.
It's the small one. Thanks!!! I am in AZ if anyone here has one, I can come
pick up.. Thanks!! Jill
Jill Cox HT (ASCP)
I vote for the scotch!
Claire
depends. A fine Scotch whiskey may be aged in old oak casks, while a
delicate Tequila may not be
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Histonet@lists.utsouthwestern.edu
Ah, in a perfect world! (which would be down right boring, thanks)
Claire
Neutral buffered formalin requires time for fixation - clots need to
be cut up as soon as possible after they're received, and biopsy
specimens really ought to fix overnight before decalcification and
processing.
Dear Todd Krueger,
There are three isomeric butyl alcohols (primary, secondary and tertiary) with
different physical properties. and different uses in histology for primary and
tertiary.
I have used tertiary butyl alcohol (= t-butanol = 2-methylpropan-2-ol) a few
times for processing into
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