Hi Naira,
We routinely do a 2 hour incubation in primary, but we make sure that more
antibody is on the slide than would be necessary for most other reagents
(500-600 ul). We have not had any issues of drying. If you are concerned
about it, just make it 2 antibody steps instead of 1 on the
Happy Friday to all,
Our processor shut off last evening in 80% alcohol due to a misconnection of
the next reagent. We restarted this morning when we arrived but the tissues
were sitting in 80% for 9 hours. My question is what affect will this have on
the tissues which are a mixture of medulla
Roger
The tissue may be sightly harder and a little more difficult therefore to
section but apart from that don't see any real problems. You may not notice any
difference.
Barry
From: histonet-boun...@lists.utsouthwestern.edu
The vasculature will leak too much and the mouse will get bloated - you'll
see it first in either the intestines blowing up like a balloon or fluid
coming out of the nose. Just not the same as the heart pumping when the
mouse is alive with intact physiology and normal functioning. Don't know
Yea I don't do incubations for longer than one hour on the autostainer and I
do put thin trays of water under the slides and on the sides of the machine
(it is very dry in Colorado), but I can think of a way you could do longer
incubations if you were around, put a coverslip or parafilm over the
Kristen,
Have you noticed the artifact in every section of a ribbon, or are some
sections better than others?
If it's in all or most of the ribbon then you might want to look at the
temperature of, or how much time the paraffin ribbon stays on the waterbath.
It may not be tearing of the
Dear Histonetters,
Thanks everyone for several good advices.
I'll try all suggestions: increase volume from 600ul to 800ul, add more primary
antibody step and put hot water dishes inside of machine to keep humidity as
high as possible.
Hopefully it will work...
Many thanks,
Naira
Does anyone have a recommendation for learning IHC for H pylori?
Nice product?
Nice protocol?
Nice learning tool?
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Thanks to those of you who sent me Magical Spreadsheets for Calculating
Histo Costs. I highly recommend the one that Tim Morken did for a
presentation at 2007 NSH - it is excellent and covers every conceivable
scenario and allows you to calculate anything. I hesitate to forward it
without his
Check the archives, I recently responded to Histonet on this very topic.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Thanks Sara, I'm glad you find it useful. I give out the spreadsheet to anyone
who wants it and you can pass it on to whoever wants it. It was part of several
NSH workshops I and Jan Gardner gave on cost accounting so those who attended
those workshops received a copy of it as well.
Tim Morken
The 3rd Annual NSH Summer Symposium will be held in Indianapolis, IN
this year on June 14-15, 2010. Be the leader in the histo-race and come
to Indy to get the education and tools needed to stay ahead. You can
earn up to 10 continuing education hours during this 2 day event.
On-line
Hi there,
I'm wondering if anyone has a histologic image of an artery from a case of
fibromuscular dysplasia (FMD) that I could borrow.
thanks,
Lise
***CONFIDENTIALITY NOTICE***
This electronic message and any attachments are intended only for the use of
the addressee and may contain
I have requested fungus control from the NSH bank, and now I'm asking
you guys... do you have non-Aspergillus controls? Our pathologists do
not like us to use Aspergillus and we are looking for Histoplasmosis if
anyone would have any to share.
Thanks and Happy Friday! j
Joyce Weems
Pathology
We are a thriving dermatopathology laboratory in operation for 15 years,
with 4 pathologists and 6 histology technicians, processing 25,000
biopsies/year. The supervisor oversees all laboratory functions, with the
primary role being participation with and supervision of our excellent team
of
Panama City, Florida seeking experienced Histotechnician for new lab in
dermatology practice. Responsibilities include grossing, processing,
embedding, cutting, staining, and cover-slipping of skin specimens.
HT(ASCP) certification preferred, no Florida license required. Lab is
located on the
Kristen
Are the tissues coming from the same client or is it all derms across the board
and just some of them? To me if the tissue is separating at the
dermal/epidermal junction, that saline or some sort of salt solution is
involved. Just a thought.
Robyn
Date: Thu, 18 Mar 2010
The hardtissue group at Yahoo! Groups is good to go.
Here are the details on hardtissue:
Group home page: http://groups.yahoo.com/group/hardtissue
Group email address: hardtis...@yahoogroups.com
Ready to start? Send an e-mail to my gmail account - clqsousa - and I will
add you as a member.
Then,
Does anyone out there have a current supplier for Edge-Rite blades? Thank
you,
Jennifer MacDonald
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On Assignment Healthcare is currently looking for a Histo Tech for a contract
assignment here in the Bay Area.
This assignment would start ASAP and will be for an indefinite period of time
as it is to covering for a leave of absence.
Hours are: Monday through Friday, 5:30am to 2:00pm.
For
http://www.bmwci.yaad.net/6kt1xp9zdw.html
_
Hotmail: Trusted email with powerful SPAM protection.
I can't believe I mispelled hard tissue on the subject of my first
message!
Sorry about that!
I have sent an invite to all of you who responded. Thank you all!
Have a great weekend,
CB
p.s.: Generally speaking this is a new e-group independent from histonet,
dedicated to connect people who work
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