RE: [Histonet] Removing tissues from OCT for molecular analysis

Katie, Rinse the frozen OCT surrounded tissue in Hanks or similar cell culture fluid. Two to three times should be enough to remove the OCT Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's h

[Histonet] MO Society for Histotechnology 2010 Spring Symposium

Hi all! The Missouri Society for Histotechnology cordially invites you to our 2010 Spring Symposium for continuing education and histopathology technique and earn up to 12 CEUs. This year's conference will be held in: Saint Louis, Missouri Thursday, May 20th to Saturday, May 22nd We will stay

[Histonet] Learn From the Best, Head West!

The Arizona Society for Histotechnology (ASH) is hosting the Region VII Summer Symposium June 4 - June 6 2010 at the Embassy Suites Phoenix North, 2577 W. Greenway Road, Phoenix, AZ 85023. Attendees with paid registration by May 15, 2010 will get a chance to win $100.00 cash. Guest speakers:

[Histonet] osteopontin

Can anyone recommend an osteopontin antibody for inflammation that works in FFPE mouse tissue, in particular mouse paws? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you a

[Histonet] Re: Acetone Fixation

Atoska Gentry asked: hello, if any of you have acetone fixation incorporated into you frozen section H& E staining protocol will you please advise me on adjustments necessary for routine staining protocol. Also, out of curiosity please enlighten me on the purpose for post sectioning acetone fixatio

[Histonet] Is my Leica CM 1850 cryostat haunted?

We have a Leica CM 1850 cryostat that about once every three weeks to a month, turnes itself off! Has anyone else experienced this? How do I fix it? Initially, we thought someone after hours was turning it off, maybe someone in housekeeping or a passing med tech. That was not the case. Later o

[Histonet] Coverglass

We are currently trying to find a manufacturer that may sell cover glass slips measuring 45 x 60. We found a box by Clay Adams, but can not find that size anymore in their catalog. Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryl

RE: [Histonet] mouse perfusion rate

I have perfused mice and rats at 300 mm Hg, about double physiological level, don't know what that made the flow rate. All mammals have the same blood pressure (within tolerances), so it is easier to select a suitable pressure to use than a flow rate, which varies dramatically. I look at bra

Re: [Histonet] Removing tissues from OCT for molecular analysis

What you should do ideally is sample you tissue for molecular studie first, then carry out frozen sections Malika " ... Smile it confuses people ..." On 29 Mar 2010, at 15:31, Katie Crosby wrote: I would like to remove tissues from blocks of OCT and perform molecule studies. I need to

[Histonet] RELIA Special Job Alert for Managers and Supervisors 3-29-10

Hi Histonetters!, I have several exciting opportunities for experienced Managers, and Supervisors in hospital and private lab environments in several locations nationwide. These are some of the premier employers in the United States. The positions are of course full time and permanent. My clien

[Histonet] RE:cover slips

I have tried several brands all have the same problem some worse than others. But even worse than the coverslips are the slides. I cut a lot of frozen sections on charged slides for fluorescent labeling the slides are dirty and the dirt gets trapped under the tissue as well as around it. The d

[Histonet] Removing tissues from OCT for molecular analysis

Hello, Can someone suggest a means of removing tissues from OCT for subsequent molecular analysis? I did not find anything in the archives describing this exactly. Thanks, Katie Katie Crosby Immunohistochemistry Cell Signaling Technology 3 Trask Lane Danvers, MA 01923 Phone: 97

Re: [Histonet] mouse perfusion rate

Hi Joe, Thanks for that notice about flow rates. But I think for the mouse you meant 1-3mls/min (not per 10min?)... Regards, Merced --On Saturday, March 27, 2010 5:03 PM -0700 Joseph Saby wrote: All- From previous work with rat perfusions, the flow rate was about 10 ml/minute. If I h

Re: [Histonet] Used knife sharpeners

Caroline, You can buy a new or refurbished HI-76 knife sharpener thru Hacker Instruments and it will come with a warranty. If you find one on Ebay, you can get the replacement honing and stropping wheels from Hacker. ( I know, it's quite the name for a company that sells knife sharpeners).