Katie,
Rinse the frozen OCT surrounded tissue in Hanks or similar cell culture
fluid. Two to three times should be enough to remove the OCT
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's h
Hi all!
The Missouri Society for Histotechnology cordially invites you to our 2010
Spring Symposium for continuing education and histopathology technique and earn
up to 12 CEUs.
This year's conference will be held in:
Saint Louis, Missouri
Thursday, May 20th to Saturday, May 22nd
We will stay
The Arizona Society for Histotechnology (ASH) is hosting the Region VII
Summer Symposium
June 4 - June 6 2010 at the Embassy Suites Phoenix North, 2577 W.
Greenway Road,
Phoenix, AZ 85023.
Attendees with paid registration by May 15, 2010 will get a chance to
win $100.00 cash.
Guest speakers:
Can anyone recommend an osteopontin antibody for inflammation that works
in FFPE mouse
tissue, in particular mouse paws?
This communication is intended solely for the use of the addressee and may
contain information that is legally privileged, confidential or exempt from
disclosure. If you a
Atoska Gentry asked:
hello, if any of you have acetone fixation incorporated into you frozen
section H& E staining protocol will you please advise me on adjustments
necessary for routine staining protocol. Also, out of curiosity please
enlighten me on the purpose for post sectioning acetone fixatio
We have a Leica CM 1850 cryostat that about once every three weeks to a month,
turnes itself off! Has anyone else experienced this? How do I fix it?
Initially, we thought someone after hours was turning it off, maybe someone in
housekeeping or a passing med tech. That was not the case.
Later o
We are currently trying to find a manufacturer that may sell cover glass
slips measuring 45 x 60. We found a box by Clay Adams, but can not find
that size anymore in their catalog.
Debra Ann Ortiz
Chief Medical Technologist
The University of Chicago Medical Center
Room E-602-A
5841 S. Maryl
I have perfused mice and rats at 300 mm Hg, about double physiological
level, don't know what that made the flow rate. All mammals have the
same blood pressure (within tolerances), so it is easier to select a
suitable pressure to use than a flow rate, which varies dramatically.
I look at bra
What you should do ideally is sample you tissue for molecular studie
first, then carry out frozen sections
Malika
" ... Smile it confuses people ..."
On 29 Mar 2010, at 15:31, Katie Crosby wrote:
I would like to remove tissues from blocks of OCT and perform
molecule studies. I need to
Hi Histonetters!,
I have several exciting opportunities for experienced Managers,
and Supervisors in hospital and private lab environments in several
locations nationwide. These are some of the premier employers in the United
States. The positions are of course full time and permanent. My clien
I have tried several brands all have the same problem some worse than others.
But even worse than the coverslips are the slides. I cut a lot of frozen
sections on charged slides for fluorescent labeling the slides are dirty and
the dirt gets trapped under the tissue as well as around it. The d
Hello,
Can someone suggest a means of removing tissues from OCT for
subsequent molecular analysis? I did not find anything in the
archives describing this exactly.
Thanks,
Katie
Katie Crosby
Immunohistochemistry
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
Phone: 97
Hi Joe,
Thanks for that notice about flow rates. But I think for the mouse you
meant 1-3mls/min (not per 10min?)...
Regards,
Merced
--On Saturday, March 27, 2010 5:03 PM -0700 Joseph Saby
wrote:
All-
From previous work with rat perfusions, the flow rate was about 10
ml/minute. If I h
Caroline,
You can buy a new or refurbished HI-76 knife sharpener thru Hacker
Instruments and it will come with a warranty. If you find one on Ebay, you
can
get the replacement honing and stropping wheels from Hacker. ( I know, it's
quite the name for a company that sells knife sharpeners).
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