I agree. We never re-use them at my facility.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Stacy
McLaughlin
Sent: Thursday, July 22, 2010 3:38 PM
To: Jeff Birkner; histonet@lists.utsouthwestern.edu
Good morning everyone,
I work in a research lab and am trying to optimize a p-TBK-1 antibody; I am
running this work-up on a BondIII and a BondMax with a Dako Envision Rabbit kit
for detection and secondary. I have only seen very faint staining and am
wondering if anyone out there has worked
Dear Friends,
I am an Histology Technologist. I am having a problem here,while
sectioning am not seeing and scoring artifacts on the section but in the
microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am
not able to locate where is the problem,is
Hello all,
We inherited a Pelco Biowave 34700 (from Ted Pella) and would like to optimize
decalcification of rats knee joints, dogs rib using the Biowave. I was
wondering if anyone have a protocol for decalcification using nitric acid or
formic acid or EDTA using microwave or if you know
Of the 3 probable causes, the most likely to be is the blade edge not
uniformly sharp
René J.
--- On Fri, 7/23/10, Aazath Raj aaz...@hotmail.com wrote:
From: Aazath Raj aaz...@hotmail.com
Subject: [Histonet] Artifacts in histology section
To: histonet@lists.utsouthwestern.edu
Date: Friday, July
Hi everyone - does anyone know how effective these formalin substitutes
are at killing microorgansms9esp HG3) in the tissue fixed? I know that
Finefix states that because of the alcohol content being over 70% that
it is effective against many micro-organisms but info seems scarce on
the others
What I have used in the past is a short warm water soak, 10-15 seconds,
in the water bath, after facing in and between sections, then placing
the block back on ice. I would use a small 200 mL beaker filled with DI
water sitting inside the water, it helps reduce junk floating the bath.
Be careful
We are doing the P16 antibody from MTM Labs.
Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph: 719-365-6926
Fax: 719-365-6373
rick.garnh...@memorialhealthsystem.com
Mission: To provide the highest quality
Hello all,
From time to time and depending on what brand of adhesive
(or charged) slides I am using, I seem to get a bag of
water that drains to the bottom of my sections but doesn't
drain out.
I have been working in microtomy a long time and have had to
deal with this contingency time and time
Hi all,
I'm a summer student and the project I was given was to optimize the
immunohistochemistry in my lab. BUT I'M NOT GETTING ANYTHING TO WORK!!
I've been told it always worked beforeSo I was wondering if anyone has
any ideas or protocols that work? I'd really appreciate any help I can
I routinely flick my wrist holding the slide with the section I've
just picked up. Usually, this is enough force to release any water at
the bottom of the section. If that doesn't work I melt a tiny hole at
the bottom of the section with a heated probe and flick again. I'm sure
the
I experienced it almost daily and thought this was normal!?
I just dipped the slides gently on a paper towel right after getting
them on the slide and shaking, for the case I could not just shake it
off. Indeed, as I remember, the Super Frost Plus slides had to be dipped
a bit harder to let
I second Linda's technique, the only other option I use is to bring the
slide up at a negative angle so the slide comes out of the water bath first
then the section. I find this works well for us.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
I use FisherBrand SuperFrost Plus charged slides, the only time I have this
problem is when my waterbath is too hot, or if for some reason I use warmed
slides to retrieve my sections from the bath (like if you lay the unused slides
on the edge of the waterbath, or if you use the warm droplet
Am I the only person who paraffin sections by: cutting the sections,
grabbing the ribbon with forceps or paintbrush, placing it on a dry slide,
adding water underneath the ribbon with a pastuer pipet, and then placing
the slide on a slide warmer. The water will heat up and the paraffin ribbon
I have always soaked my biopsies on ice water. It always seems to help.
Sent from my Verizon Wireless BlackBerry
-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Fri, 23 Jul 2010 13:01:46
To:
Always, Always, Always pH your buffers when you make them and adjust them
properly. If you do not you will NEVER know if your buffer is really at the
pH you think it is. You can't pour out 10 liters of water exactly the same
every time. You can't be sure the factory weighed the salts perfectly
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