How are institutions handling the requirement for defrosting the cryostat
during disinfection? Do most sites have a spare cryostat to use when one is
defrosting or do you pay staff to come in on the weekends to do this? I know
this requirement has been out for over a year and hoping for some
We have a second cryostat. This is also used during our scheduled pm's each
year when a contracted company comes in for this purpose. Since we have frozens
most days, we really can't be without a cryostat.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hello,
Has anyone ever used SDS along with trition in ICC protocols?
We see very different results when we use Triton alone without SDS.
Thanks!!
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Our cryostat is a CM3050--we let it defrost overnight with the glass door
open and it's fine the next morning. alternatively, you could defrost it
over a weekend. i don't know how long disinfecting takes, though, so this
may not be feasible for you.
also, it only takes a few hours more to get
With one cryostat it is a little bit difficult, but not impossibl= e.
Turn it off at the end of the day (after all the possible frozen s
urgeries are done) and let it defrost overnight. Have someone come
in= an hour or so early to clean it out with 100% alcohol (or formalin
Hello Histonetters!,
How are you doing? Did you make it to the NSH in Seattle? If so how
was it? What was your favorite thing about it? I havent been since
the convention in 2005 in Ft. Lauderdale. I really enjoyed meeting
people, learning new things and the party at the Hard Rock Casino.
The best solution is to buy a self-defrosting cryostat. They work in the same
way as your home freezer and do not build-up ice.
René J.
--- On Tue, 10/12/10, Emily Sours talulahg...@gmail.com wrote:
From: Emily Sours talulahg...@gmail.com
Subject: Re: [Histonet] Defrosting Cryostat
To:
Does anyone have any experience with storing formalin fixed breast tissue in
70% before processing? I am trying to comply with the new guidelines set forth
by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate
on the weekend we have been well above the 48 hour
We have decided to reflex to FISH those breasts that do not fall within the
recommended formalin fixation time. We do work on Saturdays so it is only the
rare 3 day weekends that this comes into play.
Jan M
Omaha
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Even with a self-defrosting cryostat, if you are regulated by CAP= you
still have to decontaminate it.
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 E= ast Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original
CAP deals with disinfecting/cleaning that has to be done daily. They do not
care about defrosting that was the core of the initial comment.
René J.
--- On Tue, 10/12/10, sgoe...@xbiotech.com sgoe...@xbiotech.com wrote:
From: sgoe...@xbiotech.com sgoe...@xbiotech.com
Subject: RE: [Histonet]
I don't think it matters if you do Her2 by FISH or IHC the time is still
48hr. I hope I am wrong, but I don't think I am.
Mike
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent:
Formalin fixation time does not impact the results of FISH as it does IHC.
Jan M
Omaha
-Original Message-
From: Mike Pence [mailto:mpe...@grhs.net]
Sent: Tuesday, October 12, 2010 11:00 AM
To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New
And the 48 hours is critical to the patient who might qualify for a clinical
trial. If it is not met the patient will not be allowed to participate. J
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 -
Sorry Jan,
ASCO/CAP Guideline Recommendations for HER2 in Breast Cancer Article
Table 6. Sample Exclusion Criteria to Perform or Interpret a HER2 FISH Assay
Item #3 Tissue fixed for prolonged intervals in formalin (greater than 48 hours)
Teresa Hallada MTCT (ASCP)
MidMichigan Health Gratiot
Thank you.
Jan
From: teri.hall...@midmichigan.org [mailto:teri.hall...@midmichigan.org]
Sent: Tuesday, October 12, 2010 11:57 AM
To: Mahoney,Janice A; mpe...@grhs.net; joyce.cl...@wchsys.org;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
I disagree. Prolonged formalin fixation (over 48 hrs), diminishes
signals
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent: Tuesday, October 12, 2010 12:05 PM
To: 'Mike Pence'; Joyce
I have a few questions regrading processing of prostate needle biopsies.
1. What is optimal fixation time that the needle cores should be fixed for
before loading these on the processors.
2. Our cores are processed on Tissue tek Xpress x120 but the morphology is not
so good. I'd like to know
What is the best embedding center? Also, is anyone using the Biocare
Autostainer and if so how do you like it.
Thanks
From: Maryott, Bridget bridget.mary...@ventana.roche.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Mon,
Hello all,
I using formalin fixed paraffin embedded specimens from fetuses from
sheep to examine which cells are virally infected within the CNS.
I am using IBA-1 to identify microglial cells. The IBA-1 antibody is a
rabbit antibody and it works really well in my specimens.
At the same time I
In the summer of this year, ASCO/CAP issued new guidelines for proper handling
of breast specimens to improve the accuracy of ER and PR status. It states that
the fixation of breast specimens must extend for at least six hours and no more
than 72 hours. It stands to reason that these guidelines
I do not see anything in this article that states the CAP will accept
72hr fixation for HER2.
-Original Message-
From: Cazares, Ruth [mailto:rcaza...@schosp.org]
Sent: Tuesday, October 12, 2010 1:18 PM
To: teri.hall...@midmichigan.org; janice.maho...@alegent.org; Mike
Pence;
Yes, but they didn't include HER2 officially. Those guidelines are still to
come is my understanding.
And then there is documenting the cold ishemic time. The nursing staff here
have been really good about it. They have to document when it was removed from
the body and when it was put in
Aline,
Jackson Immunoresearch provides protocol and reagents that are useful
for solving your problem:
http://www.jacksonimmuno.com/technical/fab-blok.asp
I have used this approach to localize at the same sample simultaneously
two different isoforms of a transcription factor with two different
Nilesh:
1- if you are fixing with NBF do not think that because the biopsies are small
require less time in the fixative. An acceptable fixing time will be 8 hours
2- the XpressX120 should give you good results IF the biopsies are well fixed.
3- Harris is a better hematoxylin because it is
I am out of the office from Tue 10/12/2010 until Thu 10/21/2010.
I will have limited access to emails during this time. If you should need
assistance, please contact Demaris Mills,
demaris.mi...@leica-microsystems.com, for product management support or
Karen Niewerth,
Hi all,
We have a very small University lab. We don't work with human tissue, so we
don't actually need to show record of maintenance.
We would like to cover our histology equipment with a maintenance contract
just in case because it is mostly old.
Does someone in LA area offer maintenance
Does anyone know where I can get this dye? It is to be used by a
research doc - he plans on putting the tissue in this dye and then into
10% formalin.
Laurie Colbert
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The ASCO/CAP Guidelines for ER/Pr may indeed eventually include Her2 but
that has not been published yet as far as I know so we still have to wait
for the 6-72 hr fixation guidelines to be decreed for Her2, now for Her2 it
is 6-48 hours. They are asking that you record cold ischemic time and time
The problem here is that the studies have not been done or at least not
published to say if 6-72 hours fixation in formalin is what we should be
using for ER/PR/Her2. The ASCO/CAP Guidelines for ER/PR (not Her2) were
based upon literature searches not lab data. The only reference I found
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