RE: [Histonet] PAS with Diastase Digestion

2011-02-10 Thread Susan.Walzer
Not spit ,saliva and we leave it on for 15/20 minutes and then rinse well. I have had techs who did not like doing it so we bought the enzyme and after all the hassle she found it did not work as well...now we all think about chocolate and salivate. :) -Original Message- From:

RE: [Histonet] FFPE tissue - sucrose fixing necessary?

2011-02-10 Thread Margaret Blount
This sounds like something you would do before freezing not processing to paraffin. Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079

Re: [Histonet] IHC validation

2011-02-10 Thread BSullivan
Added to this.make sure you keep your work up sheets. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper

RE: [Histonet] CPT code 88363

2011-02-10 Thread Mike Pence
Is this a code that can be used even if you have your molecular done somewhere else? Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Wednesday, February 09, 2011 2:43 PM To:

RE: [Histonet] QIHC

2011-02-10 Thread Houston, Ronald
Read and digest the Dako book and you will sail through the exam. For a specialist type qualification it really is pitiful. Ronnie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Bradley Sent:

RE: [Histonet] QIHC

2011-02-10 Thread Stephanie Rivera
Hi Michael, I used NSH 5th series assessment book, double check on the NSH website. It's the book with questions and answers for IHC, Enzyme histochemistry. I also used IHC tutorial by Thermo Shandon online, that was recommended on the histonet network. I'm not sure of the website. Maybe

[Histonet] Cassette labeling

2011-02-10 Thread Nancy Schmitt
We use B. Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA Message: 9 Date: Tue, 8 Feb 2011 16:54:54 -0500 From: Lee Mayhew lmayh...@cogeco.ca Subject: [Histonet] Cassette labeling To: Histonet Histonet@lists.utsouthwestern.edu Message-ID:

[Histonet] Supervisory/Lead Job in Fort Myers, FL

2011-02-10 Thread Alyssa Peterson
*Position Title: *Histotech III (Lead)/Supervisory *Reports To: *Laboratory Director *Shift: *Monday-Friday, 8am-5pm * * *Location:* * * Pathology Laboratory for well established busy office in Fort Myers, FL founded in 2000. We are looking at local candidates for now, but may be willing

Re: [Histonet] QIHC

2011-02-10 Thread Mark Tarango
Its true, that test is too easy if you ask me. Dako handbook is all you'll need. Mark On Thu, Feb 10, 2011 at 5:46 AM, Houston, Ronald ronald.hous...@nationwidechildrens.org wrote: Read and digest the Dako book and you will sail through the exam. For a specialist type qualification it

[Histonet] Histotech 2 position available in Portland, OR with Providence Health and Services

2011-02-10 Thread Fletcher, Annette M
Dear Histology Professionals, (Please, no Recruiters) If you are interested in exploring a move to the Pacific NW, please email me regarding the following opportunity: Providence is calling a full-time Histotechnician II for an evening shift position at Providence Portland Medical Center in

[Histonet] p16 antibody

2011-02-10 Thread Michele Carr
Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM? Thanks in advance. Michele Carr HTL ASCP Medical Laboratory Services ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] p16 antibody

2011-02-10 Thread Victoria Baker
Yes. They hold the patent. On Feb 10, 2011 1:30 PM, Michele Carr michelecar...@yahoo.com wrote: Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM? Thanks in advance. Michele Carr HTL ASCP Medical Laboratory Services ___

RE: [Histonet] p16 antibody

2011-02-10 Thread Walter Benton
MTM has the patent rights so they are the only one. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md

Re: [Histonet] p16 antibody

2011-02-10 Thread Mark Turner
They hold the patent and no one else produces it. We order the kit and use the antibody on the Ventana XT stainers, placing the antibody in a prep kit. Works great. Mark Turner, PhD, HT(ASCP)QIHC Mayo Clinic in Arizona Michele Carr michelecar...@yahoo.com wrote: Hi everyone was

[Histonet] tungsten carbide knives

2011-02-10 Thread Adam Hacking
Hi   If anyone has any used tungsten carbide knives that they no longer need please let me know.  These should be in good shape and suitable for sharpening. I'm happy to pay a resonable price for them.   Thanks   Adam --- On Thu, 2/10/11, histonet-requ...@lists.utsouthwestern.edu

[Histonet] Position Open in NJ/NY area

2011-02-10 Thread Alyssa Peterson
Allied Search Partners is currently accepting resumes for a histotechnician/histotechnologist. Ideally we are looking for a candidate with at least 3 years experience. Location: Clifton, NJ area *Essential Functions and Duties* Must be able to work independently under minimal supervision,

[Histonet] Need help with interpretation: Gomori Trichrome / MELAS-Syndrom

2011-02-10 Thread V. Neubert
Hi all, I'm reading about OXPHOS diseases (defects in mitochondrial protein subunits of respiratory chain). RFF in MERRF stands for red ragged fibers, see: http://en.wikipedia.org/wiki/File:Ragged_red_fibers_in_MELAS.jpg I don't get what is stained there exactly. The picture shows a muscle

[Histonet] bar-coded chemical inventory

2011-02-10 Thread Seaton, Brandon W
Anyone out there using barcode systems to inventory chemicals and organize MSDS, expiration dates, lot numbers, NFPA, etc? If so, what system and how well does it work? In particular I'm curious about the CISpro system. Many thanks! Brandon This electronic message transmission contains

[Histonet] Help with OCT problem

2011-02-10 Thread Reynolds,Donna M
Has anyone ever experienced OCT blocks that cut like they are soft. I have turned the cyrostat down 10 degrees lower than usual and they are still soft. Really weird part is that I have 20 blocks in this group and some are soft and others are just fine. Tried changing angle, new blade nothing

RE: [Histonet] bar-coded chemical inventory

2011-02-10 Thread Liz Chlipala
I would be interested in that info too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504

RE: [Histonet] Help with OCT problem

2011-02-10 Thread Liz Chlipala
Donna I have seen soft or rather sticky OCT samples in the past. If you freeze in isopentane and don't let the isopentane evaporate off the samples prior to wrapping the sample in foil, that excess isopentane changes the OCT, it makes it sticky. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

RE: [Histonet] CPT code 88363

2011-02-10 Thread Richard Cartun
No; you can not bill the patient for this. You should charge the attorney for the work you do. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford,

[Histonet] Her2 requests on decal specimens

2011-02-10 Thread Martha Ward
I am hoping to get some guidance on this. Occasionally we get requests to perform HER2 on specimens that have been decaled. The revised CAP question, ANP.22998 specifically states in the notes that decalcification with strong acids should not be used. How are other institutions handling

Re: [Histonet] Help with OCT problem

2011-02-10 Thread Patrick Laurie
Donna, I would also check to make sure that the tissue wasn't held in an something with a lot of alcohol, like RNA later. I found out first hand that it doesn't work well. Good luck On Thu, Feb 10, 2011 at 11:38 AM, Reynolds,Donna M dreyn...@mdanderson.org wrote: Has anyone ever experienced

Re: [Histonet] Help with OCT problem

2011-02-10 Thread Merced M Leiker
I agree with previous responders to this query, as I've gotten alcohol on my blocks and since alcohol doesn't freeze real well it makes a sticky mess with the OCT. The affected OCT can be scraped off with a razor blade. Hopefully the tissue itself did not come in contact with alcohol prior to

RE: [Histonet] CPT code 88363

2011-02-10 Thread Wanda.Smith
You charge the attorney's office out the waazoo!!! WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information

RE: [Histonet] Help with OCT problem

2011-02-10 Thread Ingles Claire
Same with ETOH. We wipe our surfaces down with it to clean them so no cross contamination as we freeze our skin here on the metal side areas of our cryostats. If the alcohol hasn't been wiped dry or evaporated we have that problem too. Claire From: