What would be the equivalent of a Slim Jim in Europe? The Netherlands to be
more precise?
Van: histonet-boun...@lists.utsouthwestern.edu namens Walter Benton
Verzonden: wo 16-3-2011 15:07
Aan: Hayes, Randi (HorizonNB); histonet@lists.utsouthwestern.edu
Good morning!
I'm looking for advice on an antibody conjugated with a blue fluorophore
that could be used to tag for apoptosis (on p75 receptors on cholinergic
neurons in the rat basal forebrain).
We use a Goat X ChAT primary (1:100) and a Rhodamine-conjugated Donkey X
Goat secondary (1:250) on
You can use any cassette you like if you put a blue sponge inside of it.
Some people use two sponges, one on top and one on bottom, or you can use
just one and fold it in half. Another thing you might want to try are the
small biopsy bags. They are like tiny tea bags and you just pour the
Bill,
There are plenty of secondary conjugated with blue fluorophore (check
JacksonImmunoresearch or Invitrogen catalogs), but usually these fluorophores
are not very good, signal is dull and long exposure burns signals not only in
blue channel, but in red and green as well. If your microscope
Some sort of small, snack sausage of questionable quality. I'm not familiar
with anything like that in Europe, but maybe you could determine that somehow.
Good Luck!
Tom Jasper
Bend, Oregon
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Are you the type of person who has the persistence and follow through to master
the art of Histology? Do you have great interpersonal skills to solve problems
and learn with others? Do you have an eye for detail and accuracy?
Path Logic, an expanding pathology service located in Carmichael,
As long as you order a quality paraffin from a reputable laboratory
supply company, you should be alright. I've used just about every paraffin
out there, and they are all adequate. Some are better than others, and
everyone has their personal preference. My personal favorite is Paraplast
Hi Histoneters,
I am encountering a problems when I am sectionong Chinchilla's stomach tissues(
formalin fixed paraffin embedded). Sections desintegrate, shred and scratch and
they roll up tightly.
Does anyone experienced something like this? I will appreciate any input.
Thank you
Chakib
Rena,
We just use the good old VIP from Sakura. We are a dermpath lab and do up
to 600 blocks a day. Some of our specimens are very tiny. I would check at
the grossing point first, make sure that the cassettes are being properly
closed. They have to latch tightly, or they can open during
Hi,
Has anyone used the EZ-TMA Kit for making your own tissue micro arrays?
I am trying to decide on which system to buy. So far, Quick-Ray and Arraymold
seem to be the favorites, but I would like to hear if anyone has tried EZ-TMA
before I make a decision.
Thanks!
~Ally Langsdorf
Senior
I am in the market for a new coverslipper. I am looking at Thermo's clearvue
or Leica's CV 5030. Does anyone have any input on either of these
coverslippers, good or bad.
Thank you in advance,
Allison
___
Histonet mailing list
Is anyone using this stainer and what are your thoughts? I have concerns about
the reagents being so close to the ground. This stainer also locks you into
purchasing Dako hematoxylin, bluing and eosin. Thanks.
Christie
I have used the new Leica coverslipper. I am not a fan of this
coverslipper. Too many variables with the mountant. We use what is
recommended and we either have too much and it goes on top of the coverslip
or not enough and we have air bubbles. They have been out to adjust and
still we have
It sounds like you are looking for paraffin for infiltration (not embedding).
If this is so, use a paraffin with shorter polymers like Richard-Allan Paraffin
Type 1 - The same company makes Types 3, 6, and 9 but these longer polymer
paraffins are designed for sectioning and ribbon formation.
We have this coverslipper too. Although we do not have a Ventana ihc stainer,
we sometimes coverslip our H E slides with labels on. Once the instrument was
adjusted for a different slide thickness, there was no problem.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Allison,
We have a refurbished Leica CV5030 and do like it. However, I agree with
Beatrice, it took many hours of adjusting the drop size, and the ratio of
mounting media with sovlent, to solve the mounting media problem. We did end up
buying a smaller needle to dispense the mounting media and
I agree - I have a totally Leica lab except for my Sakura tape
coverslipper and I would not trade it for anything. I test-drove the
Leica glass but it seemed that there were more minute (my-noot)
adjustments than for the Space Station and I could see problems down the
line (sorry, Leica!). I
The needles we ordered for our Leica CV5030:
The company is Intellispense-www.dispensinglink.com
They sell all sizes of re-usable (metal) luer-lok needles. We ordered the
following:
Cat. #9991258-523 gauge x 1/2 (pk of 12) - can't recall the price, very
cheap.
Peggy
Peggy Sherwood
My question is directed specifically to veterinary histologists or histologists
who also do a fair amount of animal processing. We are having a terrible time
processing pig fat. We had problems previously, but thought we had solved them.
This latest project (pig skin with a lot of fat attached)
5mm is pretty thick still. We process skin and fat and we use a longer
processing cycle. 1 to 1.5 hours per station.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
I have sent this e-mail numerous times. Please unsubscribe me at this time.
Thank You
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Just to clear the record, I do qualify for high-complexity testing, My
doctorate is in another field, and I am very proud of my accomplishments
in both areas of interest.
Thanks for all the private emails and constructive remarks regarding
this question. They have been very helpful. The
The specimens can be large, but we trim so the thickness is less than 5mm.
-Original Message-
From: Marcum, Pamela A [mailto:pamar...@uams.edu]
Sent: Thursday, March 17, 2011 4:02 PM
To: Sherwood, Margaret ; histonet@lists.utsouthwestern.edu
Subject: RE: Re: [Histonet] Processing animal
If you use a mixture of 2-propanol and mineral oil followed by pure mineral oil
to clear before infiltrating with paraffin, you will be able to cut any type
of fatty tissue.
René J.
--- On Thu, 3/17/11, Sherwood, Margaret msherw...@partners.org wrote:
From: Sherwood, Margaret
Peggy,
Is your whole program one hour or is each station one hour?
We had a project here a few years ago that we called the bacon project
because we had whole chunks of pig skin with an implant and lots of fat in
between the layers. It was fixed for a couple days in 10% NBF and then
processed
A number of companies selling histology/pathology paraffins are listed on line
or in catalogs. It will depend on what you are actually wanting as these have
been developed for routine use and the questions you have asked are taken care
by the companies. I would be happy to give you a list of
Hi,
We are asked by our safety officer to find out what the common practice is to
store 10% NBF. Is it in the chemical hood only or solvent bunkers? Can the
un-opened jars be stored in regular cabinet or on bench top? As far as I know
formalin is not flammable. Your experience and knowledge in
We buy a cubitainer of 10% Buffered formalin and keep on the benchtop for
dispensing.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chen, Shu-Cheng
Sent: Thursday, March 17, 2011 4:19 PM
To:
We trim to 2mm thick.
Jan Shivers
UMN Vet Diag Lab
- Original Message -
From: Liz Chlipala l...@premierlab.com
To: Sherwood, Margaret msherw...@partners.org;
histonet@lists.utsouthwestern.edu
Sent: Thursday, March 17, 2011 3:02 PM
Subject: RE: [Histonet] Processing animal fat
5mm
The laser treatment should not be an issue, we do that type of work all
of the time. I would trim to about 2-3 mm. I did notice that you only
have 2 changes of citrisolv a xylene substitute I have not worked with
that one in particular but if I do work with a xylene substitute we use
three
Thank you! We have been doing it the same way. But our new safety officer
thinks it is unsafe
Shu-Cheng
-Original Message-
From: Sherwood, Margaret [mailto:msherw...@partners.org]
Sent: Thursday, March 17, 2011 4:30 PM
To: Chen, Shu-Cheng; histonet@lists.utsouthwestern.edu
Hi Britt,
There are many many fluorophores you could choose from, including quite a
few that do not overlap with GFP or rhodamine. Here is a partial list:
http://flowcyt.salk.edu/fluo.html
To set up your staining, you will want to first identify a primary
antibody that labels the apoptotic cells
Thank you very much, Liz. This sounds like a good remedy to my headache. Will
look into it.
Shu-Cheng
-Original Message-
From: Liz Chlipala [mailto:l...@premierlab.com]
Sent: Thursday, March 17, 2011 5:03 PM
To: Chen, Shu-Cheng; Sherwood, Margaret ; histonet@lists.utsouthwestern.edu
Hello Histonetters,
An industry-leading IHC/ISH diagnostics company is seeking an in-house
Technical Support Representative and Field Application Specialists requiring
significant travel:
(A) Inside Technical Support Representative
Provide Technical/Applications Support on product related
34 matches
Mail list logo
