Good moorning everyone:
I am Carmen Garcia from the University of Valencia. I am just starting
my PhD. Now I have to obtain mice embryo cryocut and I am having some
problems with the morphology... We removed the embryos and put them in
sucrose 30% like 5 hours, then embebbed in OCT and put
If you do not employ recently trained individuals, how on earth are they
going to obtain the experience that Matt craves, it's beyond me. My daughter
recently qualified as an Occupational Therapist, when she asked for the
reason that she was unsuccessful in obtaining a post, they
Greetings Histoland,
in a pinch, we've also used mayonnaise. We opened one of the small packets
you get with sandwiches and smeared a little of it onto a slide and there
you go. Also, if you can obtain a fresh pierce of kidney with Renal Cell
carcinoma, that will work also.
Joe
-
You need to leave the embryos in sucrose waaay longer than 5 hours. I would
leave them at least overnight, if not more than that. You can probably take
them out of the blocks and resink them in sucrose, if you're desparate.
But next time, always sink (ie wash in sucrose) for at least overnight.
Histoland:
I have to agree also. Programs that are graduating students with none of the
skills listed in Matt's message are not doing their students or the profession
any favors. Programs certified to produce graduates should be required to
place these students in rotations that give them
Hi All,
I would like to get a list of the most common fixatives that are being used in
clinical laboratories, both in the USA as well as globally. This would be for
all types of specimens that come through your labs.
Any help would be greatly appreciated!
Thanks,
Tom
Thomas Crowell
Fellow
Good morning everybody!!
I am trying to attach iliac arteries embedded in MMA (methyl
methacrylate + dibutyl phthalate + perkadox 16) to gelatin coated slides
to allow us to stain them with the Movat's Pentachrome. Unfortunately, I
have a real problem getting them to attach - shortly after they
You might be surprised I too agree with Joyce and Richard.
I understand very well that a new graduate will not be up to the skill
level of an individual that has been working for a while. What I am
surprised about is that this program seemed to teach to pass the test
and has left all of the
Carrie Disbrow notes the use of sections of human medullary type
thyroid carcinoma as an amyloid control.
This is what I've usually seen used in recent years. Many but not all
medullary thyroid carcinomas contain amyloid. I've seen good results
with the right material.
A couple of questions I've
American Mastertech, Cat. #CSA0425P
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mesruh
turkekul
Sent: Tuesday, August 30, 2011 2:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Congo Red
Do not sweat, 85% = neutral buffered formalin. The remaining 15% are about 20
other used in such a small number of labs that do not merit consideration.
René J.
--- On Wed, 8/31/11, Crowell, Thomas thomas.crow...@novartis.com wrote:
From: Crowell, Thomas thomas.crow...@novartis.com
Subject:
Regardless of all the reasoning and good intentions, or even advantages
eliminating the practical part of the ASCP (either HT or HTL) and concentrating
in the theory only, is like graduating a medical student without doing any
hospital rotations.
I would not want to be treated by such an MD.
If
I went through histo school (in 1983-84) and we were taught the skills needed
to work in a hospital lab- Many of these came into play when we were preparing
our practical. I had to cut at my interview, being a new grad, and once I was
hired I had my 3 months probation to have the quality and
I have had one tech finish an online HT course and currently a second person
looking at going the online route to her HT. In both cases as their supervisor
I have had to sign on as their in house trainer. As a trainer it is my job to
make sure that they know how to function well in Histology
I am out of the office until 09/01/2011.
I will be out of the office with limited access to email Aug 31 to Sept 2.
For urgent matters, please contact Christina Wilson at
christina.wil...@leica-microsystems.com or 314-422-7140
Note: This is an automated response to your message Histonet
I have to agree with the fact that students should not be released into the
workforce without any practical experience. We team up with a local college
(St. Phillips College, San Antonio TX) that produces several students a year
and we along with other facilities help in the rotations for
-Original Message-
From: Galbraith, Joe
Sent: Wednesday, August 31, 2011 10:13 AM
To: 'Heath, Nancy L.'; 'histonet-boun...@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a
Pin
Nancy:
I would encourage our regional and national
Graduating students in histotech programs DO clinical rotations. They
have hands-on experience. They are not just learning the theory from
books.
Rene J Buesa rjbu...@yahoo.com
Sent by: histonet-boun...@lists.utsouthwestern.edu
08/31/2011 07:54 AM
To
Shirley A. Powell
Depending on the experiment. Our lab uses 4% PFA for cryosections,
Methanol:DMSO (4:1) for whole mount IHC, acetone/4%PFA for cell fixation
-Mehlika
Date: Wed, 31 Aug 2011 07:45:47 -0700
From: rjbu...@yahoo.com
To: histonet@lists.utsouthwestern.edu; thomas.crow...@novartis.com
Subject: Re:
Hi Carmen,
Sometimes people freeze fresh (not fixed) tissues in OCT. When freezing
fresh tissues you do not use sucrose. Sucrose is very hyper tonic and will
damage live tissue. Alternatively people freeze fixed tissues. Then
depending on the stage of the embryo you fix them in neutral buffered
Sorry for the repost- forgot to include everyone else.
Do you fix the embryos pior to sectioning? or post fix? Usually I place my
embryos in 30% sucrose over night in the 4 degree (or until they float to the
top-this also depends on the age/size of the embryos) and I fix them in 4% PFA
Will this person please contact me?
Sally Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
1101 Camino de Salud NE
Albuquerque, NM 87102
505-383-9278 (Histology Lab)
___
Histonet mailing list
Jim,
I think that I might better understand you now from your reply and would like
to share my response with others that might be following this thread. From the
looks of it you are using 50% EtOH on the slide before rolling out the section.
You then mentioned that you once used a drop of
I have followed this for a while and was wondering why no one is bringing up
the lack of Histology Schools and the small number of graduates they can
produce per year as an issue. Online is fine if you have a lab to work in.
However; one of the people no longer at this facility simply did
http://www.naacls.org/docs/Section3_HT.pdf
NAACLS spells it out. Online Schools should be following the guidelines. If
not they need to rethink their programs if they are approved by NAACLS.
sp
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I understand the NAACLS rules and live in reality where it is not always
followed due to expediency to have a registered HT. It is not the best way to
control how we train new Histologists.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I am trying to contact my LEICA rep for CONSUMABLES. Thank you to all
who've replied so far...
Sally Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
1101 Camino de Salud NE
Albuquerque, NM 87102
505-383-9278 (Histology Lab)
Yes, the old you need experience to get the job, and you need the job to get
experience
Joelle Weaver MAOM, BA, (HTL) ASCP
From: r...@leicester.ac.uk
To: mlune...@luhcares.org; histonet@lists.utsouthwestern.edu
Date: Wed, 31 Aug 2011 10:31:25 +0100
Subject: RE: [Histonet] Re:peggy
Matt, your first paragraph is exactly correct in my experience with directing a
program. And yes, it did produce very unfortunate results in some cases. Almost
to the point of negligence, I came to feel in some situations on the part of
the school ( test score focus, seemingly little concern
Looking for advice/comments/suggestions regarding sample tracking and data
management. I work for a small research institution doing mostly mouse/monkey
tissues. Up until now, we have been tracking samples with an excel
spreadsheet, but increasing workload is making this more and more
How do you become a certified HT and not have any lab experience?!
That's crazy.
Not that i know anything about being an HT, but I'm a lab tech and I can't
imagine going into the job never having been in a lab at all. What exactly
do they teach you?!
Emily
A great book should leave you with
Hello all,
I am a Master's student working on staining for pERK in 4% PFA fixed tissues.
I came across several protocols where researchers fixed their slides/tissues in
PFA and treated their slides/tissues with a methanol gradient (50% to 100%
;dehydration), followed by a ethanol
How would the expression, you need experience to get the job, and you need
the job to get experience work if a person went through flight school to be
a pilot but did not receive any actual flight training but was then allowed
to get a job as a pilot flying a commuter plane in charge of peoples
Yes, that was what I was aluding to. I try to imagine that they just don't
realize or have any knowledge of this, like me trying to fly a plane, since you
mentioned pilots. It is better for me to consider it this way than just gettig
upset or frustrated. From what I have seen in the
Hi all,
Pam speaks to the real reason we have shortages: The lack of educational and
degree-track programs at colleges and universities across the United States.
As for me, I would love to hire a shiny new HT with no practical experience.
We can give him or her that experience in our lab.
Candace,
I was much in the same boat as the other person you mentioned. I trained at
small veterinary lab and sat for the exam. When I relocated and got work in a
full service human hospital, it was culture shock. It took me a year or so to
get to where I need to be. That is why I got into
I will clarify. This person worked in the gross room as a PA and decided he
wanted an HT. So he watched over the shoulders of the histologists and learned
enough to see the basics and then studied for the exam without ever cutting or
staining a slide in Histology. His theory was - I cut
That is great that your organization does such a career ladder and fosters
professionalism. If more places adopted this, it might certainly be beneficial.
Joelle Weaver MAOM, BA, (HTL) ASCP
Date: Wed, 31 Aug 2011 12:43:30 -0500
From: sdatt...@stormontvail.org
To:
But, to take the test you need an affidavit from the pathologist that you
worked in the histology lab for at least a year. So something fishy there...
Tim Morken
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
Tim, I understand that and he got one to sign it!! So it can be done and yes
it is not legal or fair and unfortunately, it is not the first time I have
heard of it just the first time I actually could verify. Way too late when I
got here and it shows we have flaws in the system that are not
Here's a man with a beer on his mind (just barley) Some typos ar just closer
to my heart than others.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta
Sent: Wednesday, August 31, 2011
Just curious, does NAACLS perform inspections like CAP to re-accredit
and are all the programs out there NAACLS accredited?
Victor Tobias HT(ASCP)
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
They do. I think most are accredited. Seems to be some loop holes, or more
detailed process might be needed?
Sent from my Verizon Wireless BlackBerry
-Original Message-
From: Victor Tobias vic...@pathology.washington.edu
Date: Wed, 31 Aug 2011 19:35:02
To:
It seems from these comments that not all schools are created equal. I just
graduated from a one-year program in Histotechnology at Clover Park
Technical College in Lakewood, WA and I can assure you that no one graduated
unless they 1) Understood, and could preform by hand, every step in the
Me too! I am glad you felt well prepared with your program, and they set clear
standards.
Sent from my Verizon Wireless BlackBerry
-Original Message-
From: Eric Eades rcea...@gmail.com
Date: Wed, 31 Aug 2011 19:52:06
To: joellewea...@hotmail.com
Cc: thigg...@cddmedical.com;
On Aug 31, 2011, at 9:01 AM, Pam Marcum wrote:
I have followed this for a while and was wondering why no one is bringing up
the lack of Histology Schools and the small number of graduates they can
produce per year as an issue. Online is fine if you have a lab to work in.
However;
I knew I couldn't get a job in a lab without experience, so I offered myself
to anyone, anyone at all who was willing to teach me for free. Luckily I
got an awesome job for a semester (volunteering) but it taught me a lot.
You have to go out there and find yourself experience, you can't wait for
Just to let you know, I don't let new techs near prostate, liver or any other
kind of needle biopsy for at least a year. Why put them under that kind of
pressure? I want them to stay in the field. I figure working for me is torture
enough. We give new techs up to three years to get up to speed
Hello,
A few months back, there was a discussion on the list about CAP
Proficiency Test slides that weren't staining as expected. Some of you
had even contacted CAP and they acknowledged that there had been an
issue. Any of you whose PT was affected, do you mind jogging my memory
as to which
Hi Everyone-
Already traveling want to stay busy? Looking to travel? We need to add a few
and 'season' a few new techs for our travel team.
The conversation is fun--truly--and I'll answer all your questions to help you
make the right decision for you and your family.
800.756.3309
Acceptable experience, for the on-the-job route (OJT), as defined by ASCP
to sit for the HT or the HTL exam is the following:
. . . you must have experience, within the last ten years, in the
following areas:
•Fixation
•Microtomy
•Processing
•Staining
So, the PA met the requirement:
- they
Hi;
Looking and need comments on those of you that are using combination HE
stainer with glass coverslipper.
Thank you.
Ernestine Middleton, Manager, HT, HTL ,BS ,MPA
Montefiore Medical Center
Bronx, New York
718-920-4157
emidd...@montefiore.org
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