RE: [Histonet] Dako HE Stainer

I have not used it but have heard some good things. I was told that it is large (floor model?) and is best for labs with really large volumes Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From:

[Histonet] Bouin's without the picric acid?

Hello, I have been using Bouin's fixative on my fish specimens. I had simply been killing the fish, slitting them down their belly and letting them lie in Bouin's overnight. This worked well to soften the tissue and in particular the brain case (I'm interested in the brain but the dissection is

RE: [Histonet] Bouin's without the picric acid?

Tyrone, American Master Tech carries a product called Bouins 2000 which contains no picric acid. Search it on their website - they carry it in various sizes.' Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] Bouin's without the picric acid?

Hi Tyrone: Picric acid may be bad for some silver stains but not for all. Williams fix is recommended for the Bodain method and I have used it with excellent results on mouse brain. Also overnight immersion fixation of a whole fish - you did not say how large- is not going to give good

[Histonet] Pathlogix

To those of you utilizing this software. Or if I have already spoken with you about it. Please contact me throught me personal email. I have figured it out!!! Nicole Tatum nic...@dlcjax.com ___ Histonet mailing list

Re: [Histonet] Bouin's without the picric acid?

Bouin's without picric acid is an oxymoron, is like saying neutral buffered formalin without the buffer. By definition, and resulting from the original formula, Bouin requires picric acid, that is the whole rationale behind this fixative. There are commercial products called Bouin that do not

[Histonet] Re: Bouin's without the picric acid?

Bouin's without the picric acid? Bouin's fixative without picric acid isn't Bouin's fixative, any more than a chocolate cake without the chocolate is a chocolate cake. Whatever our marketing department calls Bouin's fixative is junk science. We see the same thing with bogus B-5 fixatives, and

[Histonet] Bouin's without picric acid

Tyrone - I have been working with fish (whole and pieces and parts) for years. The fixative of choice for whole fish as been Davidson's. It contains formalin, alcohol, acetic acid and water. After the whole fish is opened, the acetic acid begins to soften the harder tissues while the

[Histonet] CAP GEN.41770 Glassware cleaning

This checklist question has a sample procedure for how to check for residual detergent in glassware. The procedure says to use a pH meter. We've always used pH paper. However, in reading their recommended procedure, you need to look for a change of greater than 0.2 pH units between source

Re: [Histonet] CAP GEN.41770 Glassware cleaning

Using a pH meter requires that you add dist. water to the glass flask, swirl it around and hope that something will be trapped by that water that will give you an idea of the residual pH that may be in the glass. Both things are quite improbable. Instead of the pH meter or the paper indicator I

[Histonet] HE

Hello everybody, Can anyone help me, In Leica autostainer we have after eosin 3 100% series, clear-rite/100% and then 3 clear rites. I observed that during humid days eosin was leaching . I replaced 2 clear rite after 50 :50 Abs/cler. with xylene. The slides were fine after wards. My question is

[Histonet] Rep:Embedding centres

Hi , I suggest to go for Leica or Thermo Shandon products. Thanks Regards, S.Naveen Babu Technician - Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu