Please advise.
I was sent a bunch of sample by an investigator who is not too sharp. The
samples were prepared thus:
Pancreases were removed, washed placed in a modified Zamboni fixative (2%
formaldehyde, 15%Picric acid in 0.1M PBS, pH 7.5). Sample portions were cut
from the head, body and
The MOM kits may minimize this but they do not eliminate the non specific
binding completely in my experience, macrophages and plasma cells are
particularly difficult to eliminate staining in.
Regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
I use the tape transfer system. What tissues are you trying to cut? If you
are cutting soft tissues you should be using the 0.5 or 1x coated slides,
the 4x and above are for bone.
Have you tried putting your slides on a block of dryice after exposing them
to uv, let them sit for a while to get
I forgot to mention that I always use a D profile tungsten carbide permanent
knife to make these sections.
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-Original Message-
From: Patsy Ruegg
Re: CAP/ANP Policies
Hello All,
I am desperate for helps! I just started my new job 6 months ago, and
our lab has inspected by CAP. During their inspection, I received a
big surprise because they found no written policies for Histology in
our Q-Pulse. :( :( Obversely, we have a lot of
