Greetings, friend!
http://morcego.ind.br/response.php?ezoponiperufiv=97edawiho=258afuhaf=52
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Hi Philip,
Diatome is the brand we use. Lately they have been having
resharpening issues. We have had to send several back after
resharpening because the knives left scratch marks on the thin
sections. That said, they are friendly and helpful and have never
complained about us returning one
No, you are correct. The tech should always give the pathologists one with and
another without diastase.
René J.
--- On Thu, 3/15/12, Jennifer MacDonald jmacdon...@mtsac.edu wrote:
From: Jennifer MacDonald jmacdon...@mtsac.edu
Subject: [Histonet] PAS Diastase
To:
Have a look at Delaware Diamond Knives (DDK). Their website I believe is
www.ddk.com. I'm sure they can help you or at a minimum point you in the right
direction.
Best Regards,
Jack
On Mar 14, 2012, at 9:42 PM, Philip Slakmon slak...@yahoo.com wrote:
Good Afternoon,
I was interested in
I agree with Rene. A control (undigested) slide is necessary.
Geoff
On 3/15/2012 1:47 AM, Jennifer MacDonald wrote:
Hi All,
At a local lab when a pathologist orders a PAS diastase the
histotechnicians do just one slide with diastase. They do not do an
undigested slide. How would the
Philip,
We also use Dorn and Hart Microedge. As far as I know they only do
Tungsten Carbide and steel knives but if that is what your looking for they
are the best priced, quality, and easiest to work with.
Marc
On Thursday, March 15, 2012, Bernice Frederick b-freder...@northwestern.edu
wrote:
Hello all,
I have a question about vacuum processing.
We have ligament tissues that are processed over night, then are to be vacuum
processed. I've never done this before, but was able to get ahold of a vacuum
oven.
From what I understand, I just put the tissues in a metal container and
Hello Jennifer, I saw your message about tissue processors on the Histonet
Archive May 2006 and wondered which unit you decided to purchase? We are
looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s
and the Tissue-Tek VIP6. Your thoughts on your choice and on these
Of the 3 you mention, I would buy VIP6. Let's see what Jennifer answers.
René J.
--- On Thu, 3/15/12, John Baker bak...@umich.edu wrote:
From: John Baker bak...@umich.edu
Subject: [Histonet] Processor Preferences??
To: histonet@lists.utsouthwestern.edu
Date: Thursday, March 15, 2012, 10:13 AM
If it was me and it is in the future, I want a Leica Peloris. Leica also has a
new one that is a single chamber version of the Peloris.
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N
Vacuum processing has been advocated in the past as a way to process, but the
penetration of liquid is not a function of negative pressure (vacuum) but of
positive pressure.
In the initial experiments with the Peloris instrument in Australia (by
BioSystems before it was bought by Leica
Does anyone have a good protocol for CDC42? The ab I am using if from santa
cruz sc-8401. I stain on the dako autostainer plus using flex reagents.
Kathryn Stoll, HT(ASCP)
Department of Pathology
Medical College of Wisconsin
9200 W Wisconsin Ave
Milwaukee WI 53226
414.805.1525
kst...@mcw.edu
John,
Go for the ASP300S and then let's talk again at your convenience about resin
infiltration needs. I hope the information I sent to you was both informative
and helpful!
Regards,
Jack
On Mar 15, 2012, at 9:13 AM, John Baker bak...@umich.edu wrote:
Hello Jennifer, I saw your message
Philip,
Marc is correct in everything that he has stated, but if you are looking for
diamond knife purchases or services as you indicated in your original posting,
I am only aware of DDK providing this service.
Best Regards,
Jack
Jack Ratliff
Hard Tissue Histologist
Chairman, Hard Tissue
I disagree that two patient slides are needed. What is needed for diagnosis is
one patient slide with digestion, and two control slides, one with and one
without. If the control slide shows adequate digestion of the glycogen and the
other control slide shows adequate PAS positivity, the
Micro Star Technologies, Huntsville, Texas is also a supplier of diamond
knives and a resharpening service. I have used them many times over the
past 20+ years and have been satisfied with their product.
Jean Mitchell, BS HT (ASCP)
University of Wisconsin Hospital Clinics
Neuromuscular
Jennifer,
No you are not.
Matt Lunetta
BS HT(ASCP)
Longmont United Hospital
Message: 9
Date: Wed, 14 Mar 2012 22:47:17 -0700
From: Jennifer MacDonald
Subject: [Histonet] PAS Diastase
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=US-ASCII
Hi All,
At a
It depends on the tissue-component to be shown. If you just want to see
tissue-architecture or eg. in liver mallory-bodies and glycogen doesn't play
a role, one slide would be sufficient.
Gudrun
On 3/15/2012 1:47 AM, Jennifer MacDonald wrote:
Hi All,
At a local lab when a pathologist orders a
Philip,
Try also Diatome Diamond Knives. http://www.diatomeknives.com/
Regards,
Lita Duraine
EM Technologist
Bellen Lab
HHMI- Molecular Genetics
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Does anyone have any experience with or knowledge of this antibody (CD230)? I
would appreciate any comments/literature you could share.
Thank you,
Kim
Kimberly R. Artim AST, HT (ASCP)
St Luke's Specialty Lab
77 S. Commerce Way
Bethlehem, PA 18017
Phone: 484-526-4832
Does anyone have a good protocol for pin-4 on a Ventana XT or Ultra?
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If I am validating a new tissue processor, can I just process a v
ariety of tissues and have the pathologist sign off that they are
acce= ptable, or do I need to do a parallel run with tissue on an
established pro= cessor and compare the results?
Laurie
My two cents:
I agree as well
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001,
Tony Henwood (SCHN) would like to recall the message, [Histonet] PAS Diastase.
*
This email and any files transmitted with it are confidential and intended
solely for the use of the individual or entity to whom they
No,
If you need to demonstrate that PAS positive material is glycogen and not some
other carbohydrate containing material you must do a PAS on the test case as
well.
(see my earlier Post)
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior
Hello,
I am attempting to stain fetal membranes to look at the GAG content. My
advisor suggested Safronin O, which I tried on a preliminary set of slides
(hematoxylin, fast green, then safronin O). I did get some staining and saw
a band of red staining between the layers, as expected, but the
Tony, I agree, when looking for glycogen two patient slides should be run, one
with, one without. That is not always the case. We were sometimes asked to do
PAS/D on lymphomas.
Teri
-Original Message-
From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au]
Sent: Thursday,
Hi all,
We are looking at organising wet workshops in Sydney later this year (Wet
workshop = hands on lab work).
We ran a Histochem workshop last year at our National Meeting (twice) and many
seemed happy with it.
We are after themes for further workshops eg:
Mucin Histochemistry
HE Staining
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