This antibody was the overwhelming consensus out of all the information I
received. Thanks to all for the input!! Amy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Sunday, October 21,
I have been at the same hospital for over 34 years. I have seen pathologists
come and go, and have seen changes in services like everyone has, some good and
some bad. Since workflow changes demands that we do more with less, we are
evaluating some of the extra things we do to see if some of
Hello all,
I am in need of some help with a spirochete protocol for the Leica Bond. I am
using a Biocare primary antibody. I've tried ER1 for 20 min. with 15 min. Ab
with no luck. Does anyone do this, and do you think increasing the primary Ab
time would help? I've used this same Ab before
In our hospital, the radiology techs are responsible for bringing the renal
biopsies up to the department (in saline) as soon as the procedure is obtained.
They show the specimen to the attending pathologist, who signs off on the
radiology record as sufficient. Then the histotechs divide the
Hi Sheila,
We also use the Leica Bond using the Biocare antibody. We have had success
using the RED detection kit for the spirochete with this protocol: HIER 1 (6.0)
for 20 minutes and 30 minutes in the antibody.
hopes this helps,
Lynette
From:
James,
At our institution the techs do not go to kidney or muscle bx. The resident
will deliver the fresh specimen to either directly to our lab or to grossing.
Hospitals send them in fixative and transport media that we provide to them.
My feeling is that if the techs are not there to check
Hi all,
I am looking to for approximate dilution ranges for Dako cd3 and cd79a in
canine lymph node samples for T and Bcell Lymphoma. I am using the Flex mini
kit at the moment with high pH antigen retrieval.
Regards
Stuart___
Histonet mailing list
Don't know what a Flex mini kit is - however we use Dako CD3 @ 1:100 for 60
minute / Heat retrieval steamer - ph 9.0 Tris EDTA / Vector secondaries and
enzyme label HRP
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
Hi!
Can anyone tell me, if the amplification kit of Ventana also works on IgM
antibodies.
The specification sheet isn't very clear and I must admit, that I just don't
know, if the fc of the IgG and IgM of one species is the same.
In other words, if the anti-IgG in the amplifier also binds to
Dear all,
Its been a while i asked for a doubt to all histology experts around US, but i
have not been able to get much reply (only 1 :(). I thought my doubt/e mail
might have been missed by some, so sending it again.
I would really appreciate if anybody could guide me in my problem.
Hope to
Jim: I work in a stand alone neuromuscular lab and we do go directly to the
OR's to retrieve muscle biopsy tissue.
We offer this service for a number of reasons; we maintain excellent
communication with surgery staff on muscle bx requirements; we do triage the
tissue received on-site to
I think you normally use ATPase at different pH's, which is an enzyme stain, to
differentiate muscle fibers.
At pH 10.2 it would stain Type I fibers light and then Type II Fibers dark
(all types IIa, IIb and IIc) the other pH's stratify out the type II fibers.
Liz
Elizabeth A. Chlipala, BS,
Hi there,
Just take dog's appendix or lymphnode and titrate the antibody until it looks
like the reference on www.cqpath.com
good luck!
Marcel
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of
Can anyone recommend a CD163 antibody that works on frozen human tissue? The
two different ones that I have say not for frozen sections. any information
greatly appreciated.
Mark
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